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CST3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CST3
Entrez Gene ID 1471
Full Name cystatin C
Synonyms ARMD11
General protein information
Preferred Names
cystatin-C
Names
cystatin-C
cystatin 3
cystatin-3
gamma-trace
post-gamma-globulin
bA218C14.4 (cystatin C)
neuroendocrine basic polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20p11.21

Summary The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

604312

Disorder Html: Cerebral amyloid angiopathy, 105150 (3); Macular degeneration,





The following CST3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CST3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22520 NM_000099 Homo sapiens cystatin C (CST3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00
OHu22520 NM_001288614 Homo sapiens cystatin C (CST3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu22520
Accession Version NM_000099.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 441bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cystatin-C precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY042866.1 and BC013083.1. This sequence is a reference standard in the RefSeqGene project. On Dec 24, 2013 this sequence version replaced gi:19882253. Summary: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (1) differs in the 3' UTR compared to variant 2. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2153254 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC110305.1, BI760957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000090.1
CDS187..627
Misc Feature(1)178..180(+)
Misc Feature(2)295..615(+)
Misc Feature(3)295..441(+)
Misc Feature(4)295..297(+)
Misc Feature(5)349..351(+)
Misc Feature(6)454..456(+)
Misc Feature(7)568..570(+)
Misc Feature(8)598..600(+)
Exon (1)1..429
Gene:CST3
Gene Synonym:
Exon (2)430..543
Gene:CST3
Gene Synonym:
Exon (3)544..886
Gene:CST3
Gene Synonym:
Translation
Position Chain Variation Link
28 28 c, g dbSNP:751833500
30 30 c, g dbSNP:5030707
34 34 g, t dbSNP:71334202
44 44 c, t dbSNP:556206686
45 45 a, g dbSNP:762675483
56 56 a, g dbSNP:545738806
76 76 a, g dbSNP:576763059
81 81 c, t dbSNP:553874638
107 107 a, c, g dbSNP:113065546
115 115 a, c dbSNP:73318135
138 138 -, tctctatctagctcc dbSNP:11467210
141 141 a, c dbSNP:537755579
161 161 c, t dbSNP:568763912
171 171 a, c dbSNP:11542361
177 177 c, t dbSNP:745489881
190 190 g, t dbSNP:200984369
191 191 c, t dbSNP:780817907
193 193 a, g dbSNP:11542357
198 198 c, t dbSNP:1055084
204 204 c, t dbSNP:746955131
209 209 c, g dbSNP:776109203
212 212 a, t dbSNP:777804660
219 219 c, g dbSNP:758298115
234 234 c, t dbSNP:752589196
235 235 a, g dbSNP:11542359
242 242 c, t dbSNP:113550984
252 252 c, t dbSNP:539861839
254 254 c, t dbSNP:570741281
255 255 c, t dbSNP:11542356
258 258 a, g dbSNP:368080734
259 259 a, g dbSNP:1064039
261 261 c, t dbSNP:753942448
274 274 a, g dbSNP:200245337
275 275 a, g dbSNP:760626429
284 284 c, t dbSNP:375692362
285 285 a, g dbSNP:11542363
287 287 g, t dbSNP:768056985
291 291 a, g dbSNP:6138024
295 295 g, t dbSNP:774751752
297 297 a, g dbSNP:768894776
298 298 g, t dbSNP:745537088
299 299 c, g dbSNP:11542354
302 302 c, g dbSNP:548759955
303 303 a, c dbSNP:11542365
306 306 g, t dbSNP:770495501
308 308 a, g dbSNP:746476969
316 316 a, g dbSNP:373213120
322 322 a, g dbSNP:777386439
324 324 a, g dbSNP:201089355
334 334 c, t dbSNP:748094050
345 345 a, g dbSNP:778774442
349 349 c, t dbSNP:754673481
354 354 c, t dbSNP:754114926
360 360 c, t dbSNP:766473502
362 362 -, a dbSNP:746605962
365 365 a, g dbSNP:377450166
370 370 a, g dbSNP:563244065
381 381 c, g dbSNP:546173157
382 382 a, g dbSNP:750555631
385 385 a, g dbSNP:576809508
386 386 a, g, t dbSNP:373177867
397 397 c, g, t dbSNP:11542364
398 398 c, t dbSNP:11542360
400 400 g, t dbSNP:202145575
403 403 c, t dbSNP:111341893
405 405 a, g dbSNP:377587451
409 409 a, g dbSNP:373743268
410 410 c, t dbSNP:770529736
421 421 a, c dbSNP:574152075
422 422 a, g dbSNP:11542353
426 426 a, g dbSNP:772925286
432 432 c, t dbSNP:765692764
449 449 a, g dbSNP:371124032
450 450 a, c dbSNP:760409425
452 452 a, t dbSNP:772943690
459 459 c, t dbSNP:771732636
466 466 c, t dbSNP:761370447
467 467 a, t dbSNP:28939068
472 472 c, g dbSNP:11542355
473 473 c, g dbSNP:773883603
475 475 a, g dbSNP:768528709
477 477 c, g dbSNP:749239566
479 479 c, t dbSNP:200037041
480 480 a, c, g, t dbSNP:746101235
482 482 a, g dbSNP:781340850
486 486 c, g, t dbSNP:200401591
510 510 c, t dbSNP:751630329
519 519 a, c dbSNP:777886301
521 521 a, g dbSNP:758894505
524 524 a, c dbSNP:201184716
530 530 c, g dbSNP:765711209
535 535 c, t dbSNP:1135036
539 539 a, g dbSNP:759933096
541 541 -, g dbSNP:34930898
548 548 c, t dbSNP:755513850
549 549 a, t dbSNP:145812457
554 554 a, g dbSNP:149051742
557 557 c, t dbSNP:754306266
564 564 c, g dbSNP:766840907
567 567 c, t dbSNP:1135147
570 570 c, t dbSNP:137970982
571 571 a, g dbSNP:371605207
583 583 c, t dbSNP:763523220
586 586 a, g dbSNP:762473017
592 592 a, g dbSNP:770663365
595 595 a, g dbSNP:765197140
602 602 c, t dbSNP:759445850
603 603 a, g dbSNP:77000936
608 608 a, c dbSNP:770693621
610 610 a, g dbSNP:141643699
612 612 c, g dbSNP:773433814
621 621 c, t dbSNP:201422446
622 622 a, g dbSNP:748275854
628 628 a, g dbSNP:779110225
629 629 a, g dbSNP:777687786
630 630 -, g dbSNP:770237341
630 630 g, t dbSNP:755596435
637 637 c, g dbSNP:749804609
638 638 c, t dbSNP:371538575
639 639 a, c, g dbSNP:200979591
640 640 g, t dbSNP:763764400
648 648 c, t dbSNP:757959037
654 654 c, t dbSNP:752255286
655 655 a, g dbSNP:764714963
659 659 c, g, t dbSNP:753716901
662 662 c, t dbSNP:3209261
666 666 c, g, t dbSNP:760478171
672 672 -, cc dbSNP:760055632
673 673 c, t dbSNP:11542358
675 675 c, t dbSNP:368947792
676 676 c, t dbSNP:772383690
679 679 g, t dbSNP:11542362
682 682 c, g dbSNP:555367302
710 710 a, c dbSNP:11504
717 717 c, t dbSNP:527862834
734 734 a, g dbSNP:535553789
744 744 a, c dbSNP:3199331
759 759 a, g dbSNP:1058745
777 777 c, t dbSNP:14766
780 780 g, t dbSNP:569686274
784 784 c, t dbSNP:14768
784 784 a, g dbSNP:14767
819 819 c, t dbSNP:555982384
825 825 c, t dbSNP:1059137
828 828 a, g dbSNP:539660537
834 834 c, t dbSNP:748036591
843 843 a, g dbSNP:570540057
858 858 a, c dbSNP:1059253
876 876 a, g dbSNP:55837531
879 879 a, g dbSNP:55686485

Target ORF information:

RefSeq Version NM_000099
Organism Homo sapiens (human)
Definition Homo sapiens cystatin C (CST3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000099

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22520
Accession Version NM_001288614.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 441bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cystatin-C precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY042866.1, BX647523.1 and BF509512.1. On Dec 24, 2013 this sequence version replaced gi:530425631. Summary: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (2) represents the longer variant. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2153254 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BX647523.1, BG468788.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001275543.1
CDS187..627
Misc Feature(1)178..180(+)
Misc Feature(2)295..615(+)
Misc Feature(3)295..441(+)
Exon (1)1..429
Gene:CST3
Gene Synonym:
Exon (2)430..543
Gene:CST3
Gene Synonym:
Exon (3)544..629
Gene:CST3
Gene Synonym:
Exon (4)630..2198
Gene:CST3
Gene Synonym:
Translation
Position Chain Variation Link
28 28 c, g dbSNP:751833500
30 30 c, g dbSNP:5030707
34 34 g, t dbSNP:71334202
44 44 c, t dbSNP:556206686
45 45 a, g dbSNP:762675483
56 56 a, g dbSNP:545738806
76 76 a, g dbSNP:576763059
81 81 c, t dbSNP:553874638
107 107 a, c, g dbSNP:113065546
115 115 a, c dbSNP:73318135
138 138 -, tctctatctagctcc dbSNP:11467210
141 141 a, c dbSNP:537755579
161 161 c, t dbSNP:568763912
171 171 a, c dbSNP:11542361
177 177 c, t dbSNP:745489881
190 190 g, t dbSNP:200984369
191 191 c, t dbSNP:780817907
193 193 a, g dbSNP:11542357
198 198 c, t dbSNP:1055084
204 204 c, t dbSNP:746955131
209 209 c, g dbSNP:776109203
212 212 a, t dbSNP:777804660
219 219 c, g dbSNP:758298115
234 234 c, t dbSNP:752589196
235 235 a, g dbSNP:11542359
242 242 c, t dbSNP:113550984
252 252 c, t dbSNP:539861839
254 254 c, t dbSNP:570741281
255 255 c, t dbSNP:11542356
258 258 a, g dbSNP:368080734
259 259 a, g dbSNP:1064039
261 261 c, t dbSNP:753942448
274 274 a, g dbSNP:200245337
275 275 a, g dbSNP:760626429
284 284 c, t dbSNP:375692362
285 285 a, g dbSNP:11542363
287 287 g, t dbSNP:768056985
291 291 a, g dbSNP:6138024
295 295 g, t dbSNP:774751752
297 297 a, g dbSNP:768894776
298 298 g, t dbSNP:745537088
299 299 c, g dbSNP:11542354
302 302 c, g dbSNP:548759955
303 303 a, c dbSNP:11542365
306 306 g, t dbSNP:770495501
308 308 a, g dbSNP:746476969
316 316 a, g dbSNP:373213120
322 322 a, g dbSNP:777386439
324 324 a, g dbSNP:201089355
334 334 c, t dbSNP:748094050
345 345 a, g dbSNP:778774442
349 349 c, t dbSNP:754673481
354 354 c, t dbSNP:754114926
360 360 c, t dbSNP:766473502
362 362 -, a dbSNP:746605962
365 365 a, g dbSNP:377450166
370 370 a, g dbSNP:563244065
381 381 c, g dbSNP:546173157
382 382 a, g dbSNP:750555631
385 385 a, g dbSNP:576809508
386 386 a, g, t dbSNP:373177867
397 397 c, g, t dbSNP:11542364
398 398 c, t dbSNP:11542360
400 400 g, t dbSNP:202145575
403 403 c, t dbSNP:111341893
405 405 a, g dbSNP:377587451
409 409 a, g dbSNP:373743268
410 410 c, t dbSNP:770529736
421 421 a, c dbSNP:574152075
422 422 a, g dbSNP:11542353
426 426 a, g dbSNP:772925286
432 432 c, t dbSNP:765692764
449 449 a, g dbSNP:371124032
450 450 a, c dbSNP:760409425
452 452 a, t dbSNP:772943690
459 459 c, t dbSNP:771732636
466 466 c, t dbSNP:761370447
467 467 a, t dbSNP:28939068
472 472 c, g dbSNP:11542355
473 473 c, g dbSNP:773883603
475 475 a, g dbSNP:768528709
477 477 c, g dbSNP:749239566
479 479 c, t dbSNP:200037041
480 480 a, c, g, t dbSNP:746101235
482 482 a, g dbSNP:781340850
486 486 c, g, t dbSNP:200401591
510 510 c, t dbSNP:751630329
519 519 a, c dbSNP:777886301
521 521 a, g dbSNP:758894505
524 524 a, c dbSNP:201184716
530 530 c, g dbSNP:765711209
535 535 c, t dbSNP:1135036
539 539 a, g dbSNP:759933096
541 541 -, g dbSNP:34930898
548 548 c, t dbSNP:755513850
549 549 a, t dbSNP:145812457
554 554 a, g dbSNP:149051742
557 557 c, t dbSNP:754306266
564 564 c, g dbSNP:766840907
567 567 c, t dbSNP:1135147
570 570 c, t dbSNP:137970982
571 571 a, g dbSNP:371605207
583 583 c, t dbSNP:763523220
586 586 a, g dbSNP:762473017
592 592 a, g dbSNP:770663365
595 595 a, g dbSNP:765197140
602 602 c, t dbSNP:759445850
603 603 a, g dbSNP:77000936
608 608 a, c dbSNP:770693621
610 610 a, g dbSNP:141643699
612 612 c, g dbSNP:773433814
621 621 c, t dbSNP:201422446
622 622 a, g dbSNP:748275854
628 628 a, g dbSNP:779110225
629 629 a, g dbSNP:777687786
671 671 a, g dbSNP:774282089
676 676 c, t dbSNP:770927947
677 677 a, c dbSNP:749397284
701 701 a, g dbSNP:564902589
709 709 c, t dbSNP:545119831
717 717 a, g dbSNP:773370793
740 740 a, c dbSNP:184495702
758 758 -, g dbSNP:34860933
777 777 c, t dbSNP:770054825
779 779 c, t dbSNP:6114205
795 795 a, g dbSNP:377622960
802 802 a, g dbSNP:574146876
812 812 a, c dbSNP:60852953
831 831 c, t dbSNP:114895328
844 844 -, caa dbSNP:547863728
845 845 a, c dbSNP:192654344
849 849 a, g dbSNP:781478595
860 860 a, g dbSNP:557742547
876 876 c, t dbSNP:11696996
880 880 c, t dbSNP:147046096
891 891 c, t dbSNP:553741494
892 892 a, g dbSNP:562968564
934 934 c, t dbSNP:79649312
948 948 c, t dbSNP:6036475
957 957 a, g dbSNP:550066226
972 972 a, g dbSNP:539319636
1004 1004 a, g dbSNP:376674993
1016 1016 c, t dbSNP:188772451
1068 1068 g, t dbSNP:571311131
1074 1074 -, actg dbSNP:777723317
1087 1087 g, t dbSNP:551319914
1107 1107 a, g dbSNP:528457604
1142 1142 c, g dbSNP:751086945
1186 1186 a, g dbSNP:375854310
1200 1200 a, g dbSNP:138955478
1201 1201 c, g dbSNP:529521857
1224 1224 a, g dbSNP:563694279
1253 1253 a, g dbSNP:756788036
1254 1254 c, t dbSNP:544100724
1255 1255 c, g dbSNP:184031795
1261 1261 a, g dbSNP:374753869
1266 1266 a, g dbSNP:529391706
1270 1270 a, g dbSNP:34374560
1295 1295 a, g dbSNP:752522480
1310 1310 a, c dbSNP:541076127
1326 1326 a, g dbSNP:375939374
1336 1336 a, g dbSNP:752400831
1344 1344 g, t dbSNP:572050953
1396 1396 a, t dbSNP:558521311
1403 1403 a, t dbSNP:150159101
1422 1422 c, t dbSNP:6048957
1431 1431 a, g dbSNP:6048956
1437 1437 a, g dbSNP:201464186
1507 1507 c, g dbSNP:140837441
1517 1517 a, g dbSNP:150973024
1560 1560 a, c dbSNP:565945331
1566 1566 a, g dbSNP:556037508
1591 1591 c, g dbSNP:539358260
1598 1598 c, t dbSNP:759377266
1603 1603 a, g dbSNP:142564433
1607 1607 c, t dbSNP:547285252
1628 1628 c, t dbSNP:534723535
1633 1633 c, g dbSNP:774227113
1642 1642 c, t dbSNP:6048955
1646 1646 a, c dbSNP:766322670
1648 1648 a, g dbSNP:565918977
1734 1734 a, c dbSNP:763002179
1735 1735 a, g dbSNP:140313324
1736 1736 a, g dbSNP:773324113
1741 1741 c, t dbSNP:553746678
1752 1752 a, g dbSNP:2424574
1808 1808 a, g dbSNP:192198545
1826 1826 a, g dbSNP:748379574
1838 1838 g, t dbSNP:550440236
1839 1839 a, g dbSNP:533724294
1851 1851 -, gca dbSNP:529622244
1855 1855 a, t dbSNP:564799462
1861 1861 g, t dbSNP:541731504
1863 1863 -, g dbSNP:568658371
1899 1899 a, g dbSNP:187368682
1911 1911 a, t dbSNP:564039104
1927 1927 -, c dbSNP:752961319
1935 1935 c, t dbSNP:183656551
1962 1962 a, g dbSNP:6114204
2004 2004 a, g dbSNP:371544146
2035 2035 a, c dbSNP:768837524
2040 2040 -, a dbSNP:201660891
2046 2046 a, c dbSNP:576013573
2049 2049 a, g, t dbSNP:545639442
2064 2064 a, t dbSNP:576604577
2077 2077 a, g dbSNP:193139904
2086 2086 a, g dbSNP:747432411
2121 2121 -, a dbSNP:545723359
2129 2129 a, g dbSNP:780659368
2156 2156 a, c dbSNP:533799274
2169 2169 a, c dbSNP:566084768
2185 2185 a, g dbSNP:6114203

Target ORF information:

RefSeq Version NM_001288614
Organism Homo sapiens (human)
Definition Homo sapiens cystatin C (CST3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001288614

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGGGC CCCTGCGCGC CCCGCTGCTC CTGCTGGCCA TCCTGGCCGT GGCCCTGGCC 
GTGAGCCCCG CGGCCGGCTC CAGTCCCGGC AAGCCGCCGC GCCTGGTGGG AGGCCCCATG
GACGCCAGCG TGGAGGAGGA GGGTGTGCGG CGTGCACTGG ACTTTGCCGT CGGCGAGTAC
AACAAAGCCA GCAACGACAT GTACCACAGC CGCGCGCTGC AGGTGGTGCG CGCCCGCAAG
CAGATCGTAG CTGGGGTGAA CTACTTCTTG GACGTGGAGC TGGGCCGAAC CACGTGTACC
AAGACCCAGC CCAACTTGGA CAACTGCCCC TTCCATGACC AGCCACATCT GAAAAGGAAA
GCATTCTGCT CTTTCCAGAT CTACGCTGTG CCTTGGCAGG GCACAATGAC CTTGTCGAAA
TCCACCTGTC AGGACGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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