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CTLA4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CTLA4
Entrez Gene ID 1493
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12
General protein information
Preferred Names
cytotoxic T-lymphocyte protein 4
Names
cytotoxic T-lymphocyte protein 4
CD152 isoform
celiac disease 3
cytotoxic T-lymphocyte antigen 4
cytotoxic T-lymphocyte-associated antigen 4
cytotoxic T-lymphocyte-associated serine esterase-4
cytotoxic T lymphocyte associated antigen 4 short spliced form
ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q33

Summary This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

123890

Disorder Html: {Graves disease, susceptibility to, 4} (3); {Hypothyroidism,

mRNA and Protein(s)

mRNA Protein Name
NM_001037631 NP_001032720 cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM precursor
NM_005214 NP_005205 cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor



Homo sapiens (human) CTLA4 NP_005205.2
Pan troglodytes (chimpanzee) CTLA4 XP_526000.1
Macaca mulatta (Rhesus monkey) CTLA4 NP_001038204.1
Canis lupus familiaris (dog) CTLA4 NP_001003106.1
Bos taurus (cattle) CTLA4 NP_776722.1
Mus musculus (house mouse) Ctla4 NP_033973.2
Rattus norvegicus (Norway rat) Ctla4 NP_113862.1
Gallus gallus (chicken) CTLA4 NP_001035180.1
Xenopus (Silurana) tropicalis (western clawed frog) ctla4 XP_002936576.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CTLA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CTLA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22372
NM_001037631 Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu23246
NM_005214 Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22372
Clone ID Related Accession (Same CDS sequence) NM_001037631
Accession Version NM_001037631.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 525bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF414120.1 and AC010138.6. On Jul 2, 2011 this sequence version replaced gi:83700230. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform CTLA-4delTM (also known as sCTLA4) is soluble and lacks the transmembrane domain, compared to isoform a. The exon skip represented in this variant is is based on human U90273.1, and is consistent with mouse U90270.1 and the data published in PMID:10831323 and PMID:10556814. CCDS Note: This CCDS representation is based on U90273.1 and on full-length RT-PCR evidence from PMIDs 10831323 and 10556814. This variant also has homology support from cow AF539438.1, mouse U90270.1 and rat U90271.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001032720.1
CDS156..680
Misc Feature(1)126..128(+)
Misc Feature(2)270..611(+)
Misc Feature(3)282..551(+)
Misc Feature(4)306..506(+)
Misc Feature(5)555..572(+)
Exon (1)1..264
Gene:CTLA4
Gene Synonym:
Exon (2)265..612
Gene:CTLA4
Gene Synonym:
Exon (3)613..1865
Gene:CTLA4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001037631
Organism Homo sapiens (human)
Definition Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001037631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23246
Clone ID Related Accession (Same CDS sequence) NM_005214
Accession Version NM_005214.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 672bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF414120.1 and AC010138.6. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2011 this sequence version replaced gi:83700229. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer membrane-bound isoform CTLA4-TM. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF414120.1, BC069566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT
CCCATCAATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005205.2
CDS156..827
Misc Feature(1)126..128(+)
Misc Feature(2)270..611(+)
Misc Feature(3)282..551(+)
Misc Feature(4)291..305(+)
Misc Feature(5)306..506(+)
Misc Feature(6)555..572(+)
Misc Feature(7)603..620(+)
Misc Feature(8)639..701(+)
Misc Feature(9)756..758(+)
Misc Feature(10)756..758(+)
Misc Feature(11)756..758(+)
Misc Feature(12)756..758(+)
Misc Feature(13)756..758(+)
Exon (1)1..264
Gene:CTLA4
Gene Synonym:
Exon (2)265..612
Gene:CTLA4
Gene Synonym:
Exon (3)613..722
Gene:CTLA4
Gene Synonym:
Exon (4)723..1975
Gene:CTLA4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005214
Organism Homo sapiens (human)
Definition Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005214

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT
CCCATCAATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Immune dysregulation in human subjects with heterozygous germline mutations in CTLA4
Science 345 (6204), 1623-1627 (2014)
Kuehn HS, Ouyang W, Lo B, Deenick EK, Niemela JE, Avery DT, Schickel JN, Tran DQ, Stoddard J, Zhang Y, Frucht DM, Dumitriu B, Scheinberg P, Folio LR, Frein CA, Price S, Koh C, Heller T, Seroogy CM, Huttenlocher A, Rao VK, Su HC, Kleiner D, Notarangelo LD, Rampertaap Y, Olivier KN, McElwee J, Hughes J, Pittaluga S, Oliveira JB, Meffre E, Fleisher TA, Holland SM, Lenardo MJ, Tangye SG and Uzel G.


book

Investigation of soluble and transmembrane CTLA-4 isoforms in serum and microvesicles
J. Immunol. 193 (2), 889-900 (2014)
Esposito L, Hunter KM, Clark J, Rainbow DB, Stevens H, Denesha J, Duley S, Dawson S, Coleman G, Nutland S, Bell GL, Moran C, Pekalski M, Todd JA and Wicker LS.


book

CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells
J. Clin. Invest. 124 (7), 2977-2987 (2014)
Herrmann,A., Priceman,S.J., Kujawski,M., Xin,H., Cherryholmes,G.A., Zhang,W., Zhang,C., Lahtz,C., Kowolik,C., Forman,S.J., Kortylewski,M. and Yu,H.


book

Polymorphism analysis of CTLA-4 in childhood acute lymphoblastic leukemia
Pak J Pharm Sci 27 (4 SUPPL), 1005-1013 (2014)
Hui L, Lei Z, Peng Z, Ruobing S and Fenghua Z.


book

A native soluble form of CTLA-4
Cell. Immunol. 201 (2), 144-153 (2000)
Oaks MK, Hallett KM, Penwell RT, Stauber EC, Warren SJ and Tector AJ.


book

A soluble form of CTLA-4 generated by alternative splicing is expressed by nonstimulated human T cells
Eur. J. Immunol. 29 (11), 3596-3602 (1999)
Magistrelli G, Jeannin P, Herbault N, Benoit De Coignac A, Gauchat JF, Bonnefoy JY and Delneste Y.


book

Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 molecule
J. Immunol. 152 (10), 4929-4936 (1994)
Chen C, Gault A, Shen L and Nabavi N.


book

CTLA-4 is a second receptor for the B cell activation antigen B7
J. Exp. Med. 174 (3), 561-569 (1991)
Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK and Ledbetter JA.


book

CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location
J. Immunol. 147 (3), 1037-1044 (1991)
Harper K, Balzano C, Rouvier E, Mattei MG, Luciani MF and Golstein P.


book

Human Ig superfamily CTLA-4 gene: chromosomal localization and identity of protein sequence between murine and human CTLA-4 cytoplasmic domains
Eur. J. Immunol. 18 (12), 1901-1905 (1988)
Dariavach P, Mattei MG, Golstein P and Lefranc MP.


 
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