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CTLA4 cytotoxic T-lymphocyte-associated protein 4 [Homo sapiens (human)]

Gene Symbol CTLA4
Entrez Gene ID 1493
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12
General protein information
Preferred Names
cytotoxic T-lymphocyte protein 4
Names
cytotoxic T-lymphocyte protein 4
CD152 isoform
celiac disease 3
cytotoxic T-lymphocyte antigen 4
cytotoxic T-lymphocyte-associated antigen 4
cytotoxic T-lymphocyte-associated serine esterase-4
cytotoxic T lymphocyte associated antigen 4 short spliced form
ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q33

Summary This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

123890

Disorder Html: {Graves disease, susceptibility to, 4} (3); {Hypothyroidism,
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu22372 NM_001037631 Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu23246 NM_005214 Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu22372D
Sequence Information ORF Nucleotide Sequence (Length: 525bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF414120.1 and AC010138.6. On Jul 2, 2011 this sequence version replaced gi:83700230. Summary: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform CTLA-4delTM (also known as sCTLA4) is soluble and lacks the transmembrane domain, compared to isoform a. The exon skip represented in this variant is is based on human U90273.1, and is consistent with mouse U90270.1 and the data published in PMID:10831323 and PMID:10556814. CCDS Note: This CCDS representation is based on U90273.1 and on full-length RT-PCR evidence from PMIDs 10831323 and 10556814. This variant also has homology support from cow AF539438.1, mouse U90270.1 and rat U90271.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001032720.1
CDS156..680
Misc Feature(1)126..128(+)
Misc Feature(2)270..611(+)
Misc Feature(3)282..551(+)
Misc Feature(4)306..506(+)
Misc Feature(5)555..572(+)
Exon (1)1..264
Gene:CTLA4
Gene Synonym:
Exon (2)265..612
Gene:CTLA4
Gene Synonym:
Exon (3)613..1865
Gene:CTLA4
Gene Synonym:
Translation
Position Chain Variation Link
4 4 c, g dbSNP:538504190
5 5 g, t dbSNP:764079532
124 124 c, t dbSNP:769380431
137 137 a, g dbSNP:774909973
139 139 a, g dbSNP:536149078
140 140 a, c dbSNP:764001287
142 142 a, g dbSNP:751528406
145 145 c, g dbSNP:761713812
159 159 g, t dbSNP:767352102
177 177 c, t dbSNP:201778935
178 178 a, g, t dbSNP:138279736
183 183 a, g dbSNP:146541851
192 192 c, t dbSNP:755080468
197 197 c, t dbSNP:376591332
201 201 a, g dbSNP:748599835
202 202 a, c dbSNP:772433747
204 204 a, g, t dbSNP:231775
214 214 g, t dbSNP:769368847
230 230 c, g dbSNP:16840275
230 230 -, g dbSNP:606231418
232 232 c, t dbSNP:748802696
242 242 c, t dbSNP:145950656
246 246 a, g dbSNP:774434261
249 249 c, t dbSNP:369567630
251 251 c, t dbSNP:767441580
260 260 a, c dbSNP:606231420
272 272 c, t dbSNP:760446668
291 291 a, g dbSNP:766143912
294 294 c, t dbSNP:146200342
296 296 a, g dbSNP:776726776
306 306 c, t dbSNP:606231417
307 307 a, g dbSNP:759766975
314 314 c, t dbSNP:765325921
326 326 a, g dbSNP:373393185
334 334 a, t dbSNP:752811424
346 346 a, g dbSNP:758465752
356 356 g, t dbSNP:764639840
360 360 a, g dbSNP:557116456
361 361 c, t dbSNP:757566658
363 363 c, t dbSNP:606231422
370 370 c, t dbSNP:781579729
371 371 a, g dbSNP:199943943
391 391 g, t dbSNP:754725143
395 395 a, g dbSNP:778678737
401 401 c, t dbSNP:139154557
408 408 -, tg dbSNP:775877536
412 412 c, t dbSNP:376038796
413 413 a, g dbSNP:771695723
423 423 a, g dbSNP:370443546
426 426 a, t dbSNP:746900785
427 427 c, t dbSNP:1065442
436 436 a, t dbSNP:770666846
461 461 c, g dbSNP:776440178
466 466 c, t dbSNP:759232662
467 467 a, g dbSNP:770065318
472 472 c, t dbSNP:777843969
481 481 a, g dbSNP:144988077
487 487 a, t dbSNP:763030646
507 507 c, g dbSNP:764089901
519 519 a, g dbSNP:752037577
526 526 c, t dbSNP:757773669
527 527 a, g dbSNP:372929906
539 539 c, g dbSNP:750841862
545 545 a, g dbSNP:147679342
566 566 a, g dbSNP:188862082
569 569 a, t dbSNP:376126248
576 576 c, t dbSNP:752339954
582 582 a, g dbSNP:757989570
590 590 c, t dbSNP:777300741
608 608 a, c dbSNP:746944635
627 627 a, c dbSNP:745310078
638 638 a, c dbSNP:767174634
642 642 g, t dbSNP:749973402
644 644 c, t dbSNP:755615887
660 660 c, g dbSNP:74808460
666 666 a, g dbSNP:753717111
669 669 a, g dbSNP:754809262
671 671 c, g dbSNP:778534474
676 676 c, t dbSNP:527697475
681 681 a, g dbSNP:752988825
691 691 c, t dbSNP:758600786
699 699 c, t dbSNP:778114608
708 708 c, t dbSNP:747155841
709 709 a, g dbSNP:367760388
711 711 c, t dbSNP:370517673
724 724 a, g dbSNP:771072678
725 725 c, t dbSNP:781764712
728 728 c, g, t dbSNP:746318969
729 729 a, g dbSNP:369757839
731 731 a, g dbSNP:763343304
732 732 a, g dbSNP:771778306
743 743 a, g dbSNP:373219319
745 745 a, g dbSNP:760191875
751 751 a, g, t dbSNP:34162447
752 752 a, t dbSNP:759344822
756 756 c, t dbSNP:765202021
761 761 c, g dbSNP:571127332
769 769 a, t dbSNP:758212988
815 815 c, t dbSNP:538117059
817 817 a, g dbSNP:56102377
822 822 a, g dbSNP:568556838
838 838 -, a dbSNP:762532401
841 841 a, t dbSNP:760400720
868 868 c, t dbSNP:534771273
886 886 g, t dbSNP:553888670
933 933 c, t dbSNP:780377535
935 935 a, g dbSNP:776246855
959 959 a, g dbSNP:527391998
985 985 a, g dbSNP:56083753
988 988 g, t dbSNP:539459028
1011 1011 a, g dbSNP:557736617
1046 1046 a, g dbSNP:370681295
1059 1059 a, g dbSNP:370224520
1070 1070 a, g dbSNP:543350052
1092 1092 a, g dbSNP:13384548
1102 1102 a, g dbSNP:552656051
1168 1168 c, g dbSNP:35411154
1217 1217 g, t dbSNP:540795560
1233 1233 -, atatatatatatatatatatatatatatatatatatatatat dbSNP:142863133
1233 1233 -, atatatatatatatatatatatatat dbSNP:201484258
1233 1233 -, atatatatatatatatatatatat dbSNP:201801137
1251 1251 -, atatatatatatatatatatatat dbSNP:769484972
1274 1274 c, t dbSNP:59665421
1283 1283 -, atatat dbSNP:60872763
1288 1288 -, atatatatatatatatatatatatatatatatatatatatat dbSNP:67181450
1301 1301 a, g dbSNP:180789983
1313 1313 g, t dbSNP:560235746
1334 1334 a, g dbSNP:527732902
1341 1341 a, t dbSNP:552163643
1346 1346 c, t dbSNP:763365900
1366 1366 a, g dbSNP:139105990
1403 1403 a, g dbSNP:532076705
1417 1417 a, g dbSNP:766930311
1499 1499 a, g dbSNP:752065220
1501 1501 a, g dbSNP:755295437
1531 1531 c, t dbSNP:550184057
1533 1533 c, t dbSNP:568671903
1597 1597 c, t dbSNP:571296404
1678 1678 c, t dbSNP:56217811
1685 1685 c, g dbSNP:547979414
1700 1700 c, t dbSNP:113318671
1759 1759 a, g dbSNP:55696217
1772 1772 a, t dbSNP:190211458
1854 1854 c, g dbSNP:769191487
1860 1860 c, t dbSNP:774841150

Target ORF information:

RefSeq Version NM_001037631
Organism Homo sapiens (human)
Definition Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001037631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu23246D
Sequence Information ORF Nucleotide Sequence (Length: 672bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF414120.1 and AC010138.6. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2011 this sequence version replaced gi:83700229. Summary: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer membrane-bound isoform CTLA4-TM. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF414120.1, BC069566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT
CCCATCAATTT GA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_005205.2
CDS156..827
Misc Feature(1)126..128(+)
Misc Feature(2)270..611(+)
Misc Feature(3)282..551(+)
Misc Feature(4)291..305(+)
Misc Feature(5)306..506(+)
Misc Feature(6)555..572(+)
Misc Feature(7)603..620(+)
Misc Feature(8)639..701(+)
Misc Feature(9)756..758(+)
Misc Feature(10)756..758(+)
Misc Feature(11)756..758(+)
Misc Feature(12)756..758(+)
Misc Feature(13)756..758(+)
Exon (1)1..264
Gene:CTLA4
Gene Synonym:
Exon (2)265..612
Gene:CTLA4
Gene Synonym:
Exon (3)613..722
Gene:CTLA4
Gene Synonym:
Exon (4)723..1975
Gene:CTLA4
Gene Synonym:
Translation
Position Chain Variation Link
4 4 c, g dbSNP:538504190
5 5 g, t dbSNP:764079532
124 124 c, t dbSNP:769380431
137 137 a, g dbSNP:774909973
139 139 a, g dbSNP:536149078
140 140 a, c dbSNP:764001287
142 142 a, g dbSNP:751528406
145 145 c, g dbSNP:761713812
159 159 g, t dbSNP:767352102
177 177 c, t dbSNP:201778935
178 178 a, g, t dbSNP:138279736
183 183 a, g dbSNP:146541851
192 192 c, t dbSNP:755080468
197 197 c, t dbSNP:376591332
201 201 a, g dbSNP:748599835
202 202 a, c dbSNP:772433747
204 204 a, g, t dbSNP:231775
214 214 g, t dbSNP:769368847
230 230 c, g dbSNP:16840275
230 230 -, g dbSNP:606231418
232 232 c, t dbSNP:748802696
242 242 c, t dbSNP:145950656
246 246 a, g dbSNP:774434261
249 249 c, t dbSNP:369567630
251 251 c, t dbSNP:767441580
260 260 a, c dbSNP:606231420
272 272 c, t dbSNP:760446668
291 291 a, g dbSNP:766143912
294 294 c, t dbSNP:146200342
296 296 a, g dbSNP:776726776
306 306 c, t dbSNP:606231417
307 307 a, g dbSNP:759766975
314 314 c, t dbSNP:765325921
326 326 a, g dbSNP:373393185
334 334 a, t dbSNP:752811424
346 346 a, g dbSNP:758465752
356 356 g, t dbSNP:764639840
360 360 a, g dbSNP:557116456
361 361 c, t dbSNP:757566658
363 363 c, t dbSNP:606231422
370 370 c, t dbSNP:781579729
371 371 a, g dbSNP:199943943
391 391 g, t dbSNP:754725143
395 395 a, g dbSNP:778678737
401 401 c, t dbSNP:139154557
408 408 -, tg dbSNP:775877536
412 412 c, t dbSNP:376038796
413 413 a, g dbSNP:771695723
423 423 a, g dbSNP:370443546
426 426 a, t dbSNP:746900785
427 427 c, t dbSNP:1065442
436 436 a, t dbSNP:770666846
461 461 c, g dbSNP:776440178
466 466 c, t dbSNP:759232662
467 467 a, g dbSNP:770065318
472 472 c, t dbSNP:777843969
481 481 a, g dbSNP:144988077
487 487 a, t dbSNP:763030646
507 507 c, g dbSNP:764089901
519 519 a, g dbSNP:752037577
526 526 c, t dbSNP:757773669
527 527 a, g dbSNP:372929906
539 539 c, g dbSNP:750841862
545 545 a, g dbSNP:147679342
566 566 a, g dbSNP:188862082
569 569 a, t dbSNP:376126248
576 576 c, t dbSNP:752339954
582 582 a, g dbSNP:757989570
590 590 c, t dbSNP:777300741
608 608 a, c dbSNP:746944635
622 622 c, t dbSNP:756706504
623 623 a, g dbSNP:200657280
628 628 c, t dbSNP:745734610
634 634 c, g dbSNP:778733155
644 644 c, t dbSNP:755931042
651 651 a, g dbSNP:779775271
656 656 c, t dbSNP:749059847
667 667 c, g dbSNP:768961499
671 671 a, g dbSNP:375949600
701 701 a, t dbSNP:146571801
702 702 g, t dbSNP:771987351
704 704 c, t dbSNP:199659316
708 708 a, t dbSNP:773775010
712 712 c, t dbSNP:761227535
718 718 a, g dbSNP:766875859
720 720 a, g dbSNP:199912925
737 737 a, c dbSNP:745310078
748 748 a, c dbSNP:767174634
752 752 g, t dbSNP:749973402
754 754 c, t dbSNP:755615887
770 770 c, g dbSNP:74808460
776 776 a, g dbSNP:753717111
779 779 a, g dbSNP:754809262
781 781 c, g dbSNP:778534474
786 786 c, t dbSNP:527697475
791 791 a, g dbSNP:752988825
801 801 c, t dbSNP:758600786
809 809 c, t dbSNP:778114608
818 818 c, t dbSNP:747155841
819 819 a, g dbSNP:367760388
821 821 c, t dbSNP:370517673
834 834 a, g dbSNP:771072678
835 835 c, t dbSNP:781764712
838 838 c, g, t dbSNP:746318969
839 839 a, g dbSNP:369757839
841 841 a, g dbSNP:763343304
842 842 a, g dbSNP:771778306
853 853 a, g dbSNP:373219319
855 855 a, g dbSNP:760191875
861 861 a, g, t dbSNP:34162447
862 862 a, t dbSNP:759344822
866 866 c, t dbSNP:765202021
871 871 c, g dbSNP:571127332
879 879 a, t dbSNP:758212988
925 925 c, t dbSNP:538117059
927 927 a, g dbSNP:56102377
932 932 a, g dbSNP:568556838
948 948 -, a dbSNP:762532401
951 951 a, t dbSNP:760400720
978 978 c, t dbSNP:534771273
996 996 g, t dbSNP:553888670
1043 1043 c, t dbSNP:780377535
1045 1045 a, g dbSNP:776246855
1069 1069 a, g dbSNP:527391998
1095 1095 a, g dbSNP:56083753
1098 1098 g, t dbSNP:539459028
1121 1121 a, g dbSNP:557736617
1156 1156 a, g dbSNP:370681295
1169 1169 a, g dbSNP:370224520
1180 1180 a, g dbSNP:543350052
1202 1202 a, g dbSNP:13384548
1212 1212 a, g dbSNP:552656051
1278 1278 c, g dbSNP:35411154
1327 1327 g, t dbSNP:540795560
1343 1343 -, atatatatatatatatatatatatatatatatatatatatat dbSNP:142863133
1343 1343 -, atatatatatatatatatatatatat dbSNP:201484258
1343 1343 -, atatatatatatatatatatatat dbSNP:201801137
1361 1361 -, atatatatatatatatatatatat dbSNP:769484972
1384 1384 c, t dbSNP:59665421
1393 1393 -, atatat dbSNP:60872763
1398 1398 -, atatatatatatatatatatatatatatatatatatatatat dbSNP:67181450
1411 1411 a, g dbSNP:180789983
1423 1423 g, t dbSNP:560235746
1444 1444 a, g dbSNP:527732902
1451 1451 a, t dbSNP:552163643
1456 1456 c, t dbSNP:763365900
1476 1476 a, g dbSNP:139105990
1513 1513 a, g dbSNP:532076705
1527 1527 a, g dbSNP:766930311
1609 1609 a, g dbSNP:752065220
1611 1611 a, g dbSNP:755295437
1641 1641 c, t dbSNP:550184057
1643 1643 c, t dbSNP:568671903
1707 1707 c, t dbSNP:571296404
1788 1788 c, t dbSNP:56217811
1795 1795 c, g dbSNP:547979414
1810 1810 c, t dbSNP:113318671
1869 1869 a, g dbSNP:55696217
1882 1882 a, t dbSNP:190211458
1964 1964 c, g dbSNP:769191487
1970 1970 c, t dbSNP:774841150

Target ORF information:

RefSeq Version NM_005214
Organism Homo sapiens (human)
Definition Homo sapiens cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_005214

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT
CCCATCAATTT GA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.