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CYBB cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYBB
Entrez Gene ID 1536
Full Name cytochrome b-245, beta polypeptide
Synonyms AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX
General protein information
Preferred Names
cytochrome b-245 heavy chain
Names
cytochrome b-245 heavy chain
CGD91-phox
NADPH oxidase 2
p22 phagocyte B-cytochrome
cytochrome b558 subunit beta
cytochrome b(558) subunit beta
neutrophil cytochrome b 91 kDa polypeptide
heme-binding membrane glycoprotein gp91phox
superoxide-generating NADPH oxidase heavy chain subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp21.1

Summary Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300481

Disorder Html: Chronic granulomatous disease, X-linked, 306400 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011543890 XP_011542192 cytochrome b-245 heavy chain isoform X1
NM_000397 NP_000388 cytochrome b-245 heavy chain



Homo sapiens (human) CYBB NP_000388.2
Pan troglodytes (chimpanzee) LOC465566 XP_521002.2
Macaca mulatta (Rhesus monkey) CYBB XP_001083654.1
Canis lupus familiaris (dog) CYBB NP_001093761.1
Bos taurus (cattle) CYBB NP_776460.1
Mus musculus (house mouse) Cybb NP_031833.3
Rattus norvegicus (Norway rat) Cybb NP_076455.1
Gallus gallus (chicken) CYBB NP_001093756.1
Danio rerio (zebrafish) cybb NP_956708.1
Arabidopsis thaliana (thale cress) AT1G19230 NP_173357.1
Xenopus (Silurana) tropicalis (western clawed frog) cybb NP_001025689.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CYBB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYBB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu57140 XM_011543890 PREDICTED: Homo sapiens cytochrome b-245, beta polypeptide (CYBB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu27109
NM_000397 Homo sapiens cytochrome b-245, beta polypeptide (CYBB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57140
Clone ID Related Accession (Same CDS sequence) XM_011543890
Accession Version XM_011543890.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cytochrome b-245 heavy chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGCAT GGATGATTGC ACTTCACTCT GCGATTCACA CCATTGCACA TCTATTTAAT 
GTGGAATGGT GTGTGAATGC CCGAGTCAAT AATTCTGATC CTTATTCAGT AGCACTCTCT
GAACTTGGAG ACAGGCAAAA TGAAAGTTAT CTCAATTTTG CTCGAAAGAG AATAAAGAAC
CCTGAAGGAG GCCTGTACCT GGCTGTGACC CTGTTGGCAG GCATCACTGG AGTTGTCATC
ACGCTGTGCC TCATATTAAT TATCACTTCC TCCACCAAAA CCATCCGGAG GTCTTACTTT
GAAGTCTTTT GGTACACACA TCATCTCTTT GTGATCTTCT TCATTGGCCT TGCCATCCAT
GGAGCTGAAC GAATTGTACG TGGGCAGACC GCAGAGAGTT TGGCTGTGCA TAATATAACA
GTTTGTGAAC AAAAAATCTC AGAATGGGGA AAAATAAAGG AATGCCCAAT CCCTCAGTTT
GCTGGAAACC CTCCTATGAC TTGGAAATGG ATAGTGGGTC CCATGTTTCT GTATCTCTGT
GAGAGGTTGG TGCGGTTTTG GCGATCTCAA CAGAAGGTGG TCATCACCAA GGTGGTCACT
CACCCTTTCA AAACCATCGA GCTACAGATG AAGAAGAAGG GGTTCAAAAT GGAAGTGGGA
CAATACATTT TTGTCAAGTG CCCAAAGGTG TCCAAGCTGG AGTGGCACCC TTTTACACTG
ACATCCGCCC CTGAGGAAGA CTTCTTTAGT ATCCATATCC GCATCGTTGG GGACTGGACA
GAGGGGCTGT TCAATGCTTG TGGCTGTGAT AAGCAGGAGT TTCAAGATGC GTGGAAACTA
CCTAAGATAG CGGTTGATGG GCCCTTTGGC ACTGCCAGTG AAGATGTGTT CAGCTATGAG
GTGGTGATGT TAGTGGGAGC AGGGATTGGG GTCACACCCT TCGCATCCAT TCTCAAGTCA
GTCTGGTACA AATATTGCAA TAACGCCACC AATCTGAAGC TCAAAAAGAT CTACTTCTAC
TGGCTGTGCC GGGACACACA TGCCTTTGAG TGGTTTGCAG ATCTGCTGCA ACTGCTGGAG
AGCCAGATGC AGGAAAGGAA CAATGCCGGC TTCCTCAGCT ACAACATCTA CCTCACTGGC
TGGGATGAGT CTCAGGCCAA TCACTTTGCT GTGCACCATG ATGAGGAGAA AGATGTGATC
ACAGGCCTGA AACAAAAGAC TTTGTATGGA CGGCCCAACT GGGATAATGA ATTCAAGACA
ATTGCAAGTC AACACCCTAA TACCAGAATA GGAGTTTTCC TCTGTGGACC TGAAGCCTTG
GCTGAAACCC TGAGTAAACA AAGCATCTCC AACTCTGAGT CTGGCCCTCG GGGAGTGCAT
TTCATTTTCA ACAAGGAAAA CTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542192.1
CDS407..1813
Misc Feature(1)407..760(+)
Misc Feature(2)989..1810(+)
Misc Feature(3)1070..1333(+)
Misc Feature(4)1112..1123(+)
Misc Feature(5)1316..1348(+)
Misc Feature(6)1316..1345(+)
Misc Feature(7)1331..1717(+)
Misc Feature(8)1424..1438(+)
Translation

Target ORF information:

RefSeq Version XM_011543890
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome b-245, beta polypeptide (CYBB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543890

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTGGCAT GGATGATTGC ACTTCACTCT GCGATTCACA CCATTGCACA TCTATTTAAT 
GTGGAATGGT GTGTGAATGC CCGAGTCAAT AATTCTGATC CTTATTCAGT AGCACTCTCT
GAACTTGGAG ACAGGCAAAA TGAAAGTTAT CTCAATTTTG CTCGAAAGAG AATAAAGAAC
CCTGAAGGAG GCCTGTACCT GGCTGTGACC CTGTTGGCAG GCATCACTGG AGTTGTCATC
ACGCTGTGCC TCATATTAAT TATCACTTCC TCCACCAAAA CCATCCGGAG GTCTTACTTT
GAAGTCTTTT GGTACACACA TCATCTCTTT GTGATCTTCT TCATTGGCCT TGCCATCCAT
GGAGCTGAAC GAATTGTACG TGGGCAGACC GCAGAGAGTT TGGCTGTGCA TAATATAACA
GTTTGTGAAC AAAAAATCTC AGAATGGGGA AAAATAAAGG AATGCCCAAT CCCTCAGTTT
GCTGGAAACC CTCCTATGAC TTGGAAATGG ATAGTGGGTC CCATGTTTCT GTATCTCTGT
GAGAGGTTGG TGCGGTTTTG GCGATCTCAA CAGAAGGTGG TCATCACCAA GGTGGTCACT
CACCCTTTCA AAACCATCGA GCTACAGATG AAGAAGAAGG GGTTCAAAAT GGAAGTGGGA
CAATACATTT TTGTCAAGTG CCCAAAGGTG TCCAAGCTGG AGTGGCACCC TTTTACACTG
ACATCCGCCC CTGAGGAAGA CTTCTTTAGT ATCCATATCC GCATCGTTGG GGACTGGACA
GAGGGGCTGT TCAATGCTTG TGGCTGTGAT AAGCAGGAGT TTCAAGATGC GTGGAAACTA
CCTAAGATAG CGGTTGATGG GCCCTTTGGC ACTGCCAGTG AAGATGTGTT CAGCTATGAG
GTGGTGATGT TAGTGGGAGC AGGGATTGGG GTCACACCCT TCGCATCCAT TCTCAAGTCA
GTCTGGTACA AATATTGCAA TAACGCCACC AATCTGAAGC TCAAAAAGAT CTACTTCTAC
TGGCTGTGCC GGGACACACA TGCCTTTGAG TGGTTTGCAG ATCTGCTGCA ACTGCTGGAG
AGCCAGATGC AGGAAAGGAA CAATGCCGGC TTCCTCAGCT ACAACATCTA CCTCACTGGC
TGGGATGAGT CTCAGGCCAA TCACTTTGCT GTGCACCATG ATGAGGAGAA AGATGTGATC
ACAGGCCTGA AACAAAAGAC TTTGTATGGA CGGCCCAACT GGGATAATGA ATTCAAGACA
ATTGCAAGTC AACACCCTAA TACCAGAATA GGAGTTTTCC TCTGTGGACC TGAAGCCTTG
GCTGAAACCC TGAGTAAACA AAGCATCTCC AACTCTGAGT CTGGCCCTCG GGGAGTGCAT
TTCATTTTCA ACAAGGAAAA CTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27109
Clone ID Related Accession (Same CDS sequence) NM_000397
Accession Version NM_000397.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1713bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytochrome b-245 heavy chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA995835.1 and BC032720.1. This sequence is a reference standard in the RefSeqGene project. On Dec 21, 2007 this sequence version replaced gi:6996020. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032720.1, AK289753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGAACT GGGCTGTGAA TGAGGGGCTC TCCATTTTTG TCATTCTGGT TTGGCTGGGG 
TTGAACGTCT TCCTCTTTGT CTGGTATTAC CGGGTTTATG ATATTCCACC TAAGTTCTTT
TACACAAGAA AACTTCTTGG GTCAGCACTG GCACTGGCCA GGGCCCCTGC AGCCTGCCTG
AATTTCAACT GCATGCTGAT TCTCTTGCCA GTCTGTCGAA ATCTGCTGTC CTTCCTCAGG
GGTTCCAGTG CGTGCTGCTC AACAAGAGTT CGAAGACAAC TGGACAGGAA TCTCACCTTT
CATAAAATGG TGGCATGGAT GATTGCACTT CACTCTGCGA TTCACACCAT TGCACATCTA
TTTAATGTGG AATGGTGTGT GAATGCCCGA GTCAATAATT CTGATCCTTA TTCAGTAGCA
CTCTCTGAAC TTGGAGACAG GCAAAATGAA AGTTATCTCA ATTTTGCTCG AAAGAGAATA
AAGAACCCTG AAGGAGGCCT GTACCTGGCT GTGACCCTGT TGGCAGGCAT CACTGGAGTT
GTCATCACGC TGTGCCTCAT ATTAATTATC ACTTCCTCCA CCAAAACCAT CCGGAGGTCT
TACTTTGAAG TCTTTTGGTA CACACATCAT CTCTTTGTGA TCTTCTTCAT TGGCCTTGCC
ATCCATGGAG CTGAACGAAT TGTACGTGGG CAGACCGCAG AGAGTTTGGC TGTGCATAAT
ATAACAGTTT GTGAACAAAA AATCTCAGAA TGGGGAAAAA TAAAGGAATG CCCAATCCCT
CAGTTTGCTG GAAACCCTCC TATGACTTGG AAATGGATAG TGGGTCCCAT GTTTCTGTAT
CTCTGTGAGA GGTTGGTGCG GTTTTGGCGA TCTCAACAGA AGGTGGTCAT CACCAAGGTG
GTCACTCACC CTTTCAAAAC CATCGAGCTA CAGATGAAGA AGAAGGGGTT CAAAATGGAA
GTGGGACAAT ACATTTTTGT CAAGTGCCCA AAGGTGTCCA AGCTGGAGTG GCACCCTTTT
ACACTGACAT CCGCCCCTGA GGAAGACTTC TTTAGTATCC ATATCCGCAT CGTTGGGGAC
TGGACAGAGG GGCTGTTCAA TGCTTGTGGC TGTGATAAGC AGGAGTTTCA AGATGCGTGG
AAACTACCTA AGATAGCGGT TGATGGGCCC TTTGGCACTG CCAGTGAAGA TGTGTTCAGC
TATGAGGTGG TGATGTTAGT GGGAGCAGGG ATTGGGGTCA CACCCTTCGC ATCCATTCTC
AAGTCAGTCT GGTACAAATA TTGCAATAAC GCCACCAATC TGAAGCTCAA AAAGATCTAC
TTCTACTGGC TGTGCCGGGA CACACATGCC TTTGAGTGGT TTGCAGATCT GCTGCAACTG
CTGGAGAGCC AGATGCAGGA AAGGAACAAT GCCGGCTTCC TCAGCTACAA CATCTACCTC
ACTGGCTGGG ATGAGTCTCA GGCCAATCAC TTTGCTGTGC ACCATGATGA GGAGAAAGAT
GTGATCACAG GCCTGAAACA AAAGACTTTG TATGGACGGC CCAACTGGGA TAATGAATTC
AAGACAATTG CAAGTCAACA CCCTAATACC AGAATAGGAG TTTTCCTCTG TGGACCTGAA
GCCTTGGCTG AAACCCTGAG TAAACAAAGC ATCTCCAACT CTGAGTCTGG CCCTCGGGGA
GTGCATTTCA TTTTCAACAA GGAAAACTTC TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000388.2
CDS62..1774
Misc Feature(1)17..19(+)
Misc Feature(2)86..148(+)
Misc Feature(3)254..721(+)
Misc Feature(4)263..265(+)
Misc Feature(5)314..316(+)
Misc Feature(6)368..430(+)
Misc Feature(7)569..631(+)
Misc Feature(8)662..724(+)
Misc Feature(9)845..907(+)
Misc Feature(10)950..1771(+)
Misc Feature(11)1031..1294(+)
Misc Feature(12)1073..1084(+)
Misc Feature(13)1277..1309(+)
Misc Feature(14)1277..1306(+)
Misc Feature(15)1292..1678(+)
Misc Feature(16)1385..1399(+)
Exon (1)1..106
Gene:CYBB
Gene Synonym:
Exon (2)107..202
Gene:CYBB
Gene Synonym:
Exon (3)203..313
Gene:CYBB
Gene Synonym:
Exon (4)314..398
Gene:CYBB
Gene Synonym:
Exon (5)399..544
Gene:CYBB
Gene Synonym:
Exon (6)545..735
Gene:CYBB
Gene Synonym:
Exon (7)736..865
Gene:CYBB
Gene Synonym:
Exon (8)866..958
Gene:CYBB
Gene Synonym:
Exon (9)959..1212
Gene:CYBB
Gene Synonym:
Exon (10)1213..1375
Gene:CYBB
Gene Synonym:
Exon (11)1376..1522
Gene:CYBB
Gene Synonym:
Exon (12)1523..1647
Gene:CYBB
Gene Synonym:
Exon (13)1648..4318
Gene:CYBB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000397
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome b-245, beta polypeptide (CYBB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000397

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGGAACT GGGCTGTGAA TGAGGGGCTC TCCATTTTTG TCATTCTGGT TTGGCTGGGG 
TTGAACGTCT TCCTCTTTGT CTGGTATTAC CGGGTTTATG ATATTCCACC TAAGTTCTTT
TACACAAGAA AACTTCTTGG GTCAGCACTG GCACTGGCCA GGGCCCCTGC AGCCTGCCTG
AATTTCAACT GCATGCTGAT TCTCTTGCCA GTCTGTCGAA ATCTGCTGTC CTTCCTCAGG
GGTTCCAGTG CGTGCTGCTC AACAAGAGTT CGAAGACAAC TGGACAGGAA TCTCACCTTT
CATAAAATGG TGGCATGGAT GATTGCACTT CACTCTGCGA TTCACACCAT TGCACATCTA
TTTAATGTGG AATGGTGTGT GAATGCCCGA GTCAATAATT CTGATCCTTA TTCAGTAGCA
CTCTCTGAAC TTGGAGACAG GCAAAATGAA AGTTATCTCA ATTTTGCTCG AAAGAGAATA
AAGAACCCTG AAGGAGGCCT GTACCTGGCT GTGACCCTGT TGGCAGGCAT CACTGGAGTT
GTCATCACGC TGTGCCTCAT ATTAATTATC ACTTCCTCCA CCAAAACCAT CCGGAGGTCT
TACTTTGAAG TCTTTTGGTA CACACATCAT CTCTTTGTGA TCTTCTTCAT TGGCCTTGCC
ATCCATGGAG CTGAACGAAT TGTACGTGGG CAGACCGCAG AGAGTTTGGC TGTGCATAAT
ATAACAGTTT GTGAACAAAA AATCTCAGAA TGGGGAAAAA TAAAGGAATG CCCAATCCCT
CAGTTTGCTG GAAACCCTCC TATGACTTGG AAATGGATAG TGGGTCCCAT GTTTCTGTAT
CTCTGTGAGA GGTTGGTGCG GTTTTGGCGA TCTCAACAGA AGGTGGTCAT CACCAAGGTG
GTCACTCACC CTTTCAAAAC CATCGAGCTA CAGATGAAGA AGAAGGGGTT CAAAATGGAA
GTGGGACAAT ACATTTTTGT CAAGTGCCCA AAGGTGTCCA AGCTGGAGTG GCACCCTTTT
ACACTGACAT CCGCCCCTGA GGAAGACTTC TTTAGTATCC ATATCCGCAT CGTTGGGGAC
TGGACAGAGG GGCTGTTCAA TGCTTGTGGC TGTGATAAGC AGGAGTTTCA AGATGCGTGG
AAACTACCTA AGATAGCGGT TGATGGGCCC TTTGGCACTG CCAGTGAAGA TGTGTTCAGC
TATGAGGTGG TGATGTTAGT GGGAGCAGGG ATTGGGGTCA CACCCTTCGC ATCCATTCTC
AAGTCAGTCT GGTACAAATA TTGCAATAAC GCCACCAATC TGAAGCTCAA AAAGATCTAC
TTCTACTGGC TGTGCCGGGA CACACATGCC TTTGAGTGGT TTGCAGATCT GCTGCAACTG
CTGGAGAGCC AGATGCAGGA AAGGAACAAT GCCGGCTTCC TCAGCTACAA CATCTACCTC
ACTGGCTGGG ATGAGTCTCA GGCCAATCAC TTTGCTGTGC ACCATGATGA GGAGAAAGAT
GTGATCACAG GCCTGAAACA AAAGACTTTG TATGGACGGC CCAACTGGGA TAATGAATTC
AAGACAATTG CAAGTCAACA CCCTAATACC AGAATAGGAG TTTTCCTCTG TGGACCTGAA
GCCTTGGCTG AAACCCTGAG TAAACAAAGC ATCTCCAACT CTGAGTCTGG CCCTCGGGGA
GTGCATTTCA TTTTCAACAA GGAAAACTTC TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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