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CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [Homo sapiens (human)]

Gene Symbol CYP2E1
Entrez Gene ID 1571
Full Name cytochrome P450, family 2, subfamily E, polypeptide 1
Synonyms CPE1, CYP2E, P450-J, P450C2E
General protein information
Preferred Names
cytochrome P450 2E1
Names
cytochrome P450 2E1
CYPIIE1
cytochrome P450-J
microsomal monooxygenase
xenobiotic monooxygenase
4-nitrophenol 2-hydroxylase
flavoprotein-linked monooxygenase
cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
NP_000764.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q26.3

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

124040

Disorder Html:





The following CYP2E1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP2E1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25425 NM_000773 Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$409

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu25425
Accession Version NM_000773.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu25425D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cytochrome P450 2E1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX951478.1, J02625.1 and AL161645.14. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2005 this sequence version replaced gi:13699837. Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02625.1, AF182276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCTGCCC TCGGAGTCAC CGTGGCCCTG CTGGTGTGGG CGGCCTTCCT CCTGCTGGTG 
TCCATGTGGA GGCAGGTGCA CAGCAGCTGG AATCTGCCCC CAGGCCCTTT CCCGCTTCCC
ATCATCGGGA ACCTCTTCCA GTTGGAATTG AAGAATATTC CCAAGTCCTT CACCCGGTTG
GCCCAGCGCT TCGGGCCGGT GTTCACGCTG TACGTGGGCT CGCAGCGCAT GGTGGTGATG
CACGGCTACA AGGCGGTGAA GGAAGCGCTG CTGGACTACA AGGACGAGTT CTCGGGCAGA
GGCGACCTCC CCGCGTTCCA TGCGCACAGG GACAGGGGAA TCATTTTTAA TAATGGACCT
ACCTGGAAGG ACATCCGGCG GTTTTCCCTG ACCACCCTCC GGAACTATGG GATGGGGAAA
CAGGGCAATG AGAGCCGGAT CCAGAGGGAG GCCCACTTCC TGCTGGAAGC ACTCAGGAAG
ACCCAAGGCC AGCCTTTCGA CCCCACCTTC CTCATCGGCT GCGCGCCCTG CAACGTCATA
GCCGACATCC TCTTCCGCAA GCATTTTGAC TACAATGATG AGAAGTTTCT AAGGCTGATG
TATTTGTTTA ATGAGAACTT CCACCTACTC AGCACTCCCT GGCTCCAGCT TTACAATAAT
TTTCCCAGCT TTCTACACTA CTTGCCTGGA AGCCACAGAA AAGTCATAAA AAATGTGGCT
GAAGTAAAAG AGTATGTGTC TGAAAGGGTG AAGGAGCACC ATCAATCTCT GGACCCCAAC
TGTCCCCGGG ACCTCACCGA CTGCCTGCTC GTGGAAATGG AGAAGGAAAA GCACAGTGCA
GAGCGCTTGT ACACAATGGA CGGTATCACC GTGACTGTGG CCGACCTGTT CTTTGCGGGG
ACAGAGACCA CCAGCACAAC TCTGAGATAT GGGCTCCTGA TTCTCATGAA ATACCCTGAG
ATCGAAGAGA AGCTCCATGA AGAAATTGAC AGGGTGATTG GGCCAAGCCG AATCCCTGCC
ATCAAGGATA GGCAAGAGAT GCCCTACATG GATGCTGTGG TGCATGAGAT TCAGCGGTTC
ATCACCCTCG TGCCCTCCAA CCTGCCCCAT GAAGCAACCC GAGACACCAT TTTCAGAGGA
TACCTCATCC CCAAGGGCAC AGTCGTAGTG CCAACTCTGG ACTCTGTTTT GTATGACAAC
CAAGAATTTC CTGATCCAGA AAAGTTTAAG CCAGAACACT TCCTGAATGA AAATGGAAAG
TTCAAGTACA GTGACTATTT CAAGCCATTT TCCACAGGAA AACGAGTGTG TGCTGGAGAA
GGCCTGGCTC GCATGGAGTT GTTTCTTTTG TTGTGTGCCA TTTTGCAGCA TTTTAATTTG
AAGCCTCTCG TTGACCCAAA GGATATCGAC CTCAGCCCTA TACATATTGG GTTTGGCTGT
ATCCCACCAC GTTACAAACT CTGTGTCATT CCCCGCTCAT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000764.1
CDS34..1515
Misc Feature(1)130..1500(+)
Misc Feature(2)925..942(+)
Misc Feature(3)1342..1344(+)
Exon (1)1..210
Gene:CYP2E1
Gene Synonym:
Exon (2)211..370
Gene:CYP2E1
Gene Synonym:
Exon (3)371..520
Gene:CYP2E1
Gene Synonym:
Exon (4)521..681
Gene:CYP2E1
Gene Synonym:
Exon (5)682..858
Gene:CYP2E1
Gene Synonym:
Exon (6)859..1000
Gene:CYP2E1
Gene Synonym:
Exon (7)1001..1188
Gene:CYP2E1
Gene Synonym:
Exon (8)1189..1330
Gene:CYP2E1
Gene Synonym:
Exon (9)1331..1667
Gene:CYP2E1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 -, c dbSNP:397709571
2 2 c, t dbSNP:200292747
3 3 c, t dbSNP:201519230
4 4 c, g dbSNP:775845751
5 5 c, t dbSNP:374647442
6 6 c, g dbSNP:371502619
9 9 a, c, t dbSNP:577328423
21 21 c, t dbSNP:761293223
25 25 a, g dbSNP:766977009
28 28 a, g, t dbSNP:773200181
33 33 c, t dbSNP:766143856
43 43 c, t dbSNP:753713804
45 45 c, t dbSNP:758640808
57 57 a, g dbSNP:764387026
64 64 a, c dbSNP:543066971
71 71 g, t dbSNP:757352167
74 74 c, t dbSNP:563043306
75 75 a, g dbSNP:375169910
82 82 c, t dbSNP:367957731
89 89 c, t dbSNP:780358119
90 90 g, t dbSNP:201068922
99 99 c, g dbSNP:772430702
109 109 a, g dbSNP:59868347
110 110 g, t dbSNP:769059259
115 115 a, g dbSNP:775675704
116 116 a, g dbSNP:773963522
139 139 c, g dbSNP:747825060
142 142 c, t dbSNP:771599544
143 143 -, c dbSNP:72559707
146 146 c, g dbSNP:76271067
147 147 a, g dbSNP:137875795
153 153 c, t dbSNP:766438198
156 156 a, c dbSNP:776753984
160 160 a, g dbSNP:759372423
165 165 a, c dbSNP:527949682
175 175 c, t dbSNP:369847743
181 181 c, t dbSNP:757431552
188 188 a, g dbSNP:143746211
192 192 -, c dbSNP:72559709
194 194 c, t dbSNP:750499891
202 202 c, t dbSNP:756596289
205 205 a, t dbSNP:780517566
207 207 c, g, t dbSNP:114535864
209 209 a, g dbSNP:779311330
216 216 c, t dbSNP:762634609
217 217 c, g dbSNP:768536485
218 218 a, g dbSNP:773302720
220 220 c, g, t dbSNP:374426412
223 223 -, t dbSNP:754973375
226 226 a, g dbSNP:80297454
228 228 a, g dbSNP:555927807
231 231 a, g dbSNP:755921307
240 240 a, g dbSNP:77188198
247 247 a, c, g, t dbSNP:35844228
254 254 c, t dbSNP:764575539
260 260 a, c, g dbSNP:72559710
262 262 a, g dbSNP:78902616
265 265 a, g dbSNP:756973880
270 270 a, g dbSNP:780808608
274 274 c, g dbSNP:745393299
277 277 a, g dbSNP:755735655
282 282 c, t dbSNP:779825840
284 284 a, g dbSNP:749315338
288 288 a, g dbSNP:768324519
297 297 a, g dbSNP:774211736
299 299 c, t dbSNP:747170540
321 321 c, g dbSNP:771177357
326 326 c, t dbSNP:776646693
327 327 a, g dbSNP:28371740
330 330 c, t dbSNP:765420456
336 336 c, t dbSNP:775844629
342 342 c, g dbSNP:763527031
343 343 c, t dbSNP:764296273
344 344 c, t dbSNP:543855005
345 345 c, t dbSNP:756920666
348 348 c, g dbSNP:767255883
356 356 a, c dbSNP:750140082
367 367 -, a dbSNP:778940161
380 380 g, t dbSNP:777555849
387 387 c, t dbSNP:746021573
391 391 c, t dbSNP:770084630
395 395 c, t dbSNP:779948307
400 400 a, g dbSNP:377658981
409 409 c, t dbSNP:60719153
410 410 a, g dbSNP:56864127
411 411 a, g dbSNP:143942558
412 412 c, t dbSNP:772509721
413 413 a, g dbSNP:773718909
429 429 c, t dbSNP:760207696
430 430 c, t dbSNP:148644999
434 434 a, g dbSNP:765056111
440 440 a, g dbSNP:759059865
448 448 a, g dbSNP:764780713
449 449 a, g dbSNP:752570058
455 455 a, c dbSNP:758334170
456 456 c, g dbSNP:777502721
457 457 a, g dbSNP:751390447
462 462 c, t dbSNP:763755007
465 465 c, g dbSNP:757050207
469 469 c, t dbSNP:370419762
470 470 a, g dbSNP:749539588
482 482 a, c dbSNP:200955165
489 489 a, c dbSNP:779148806
492 492 c, t dbSNP:372241274
494 494 g, t dbSNP:373427672
495 495 a, g, t dbSNP:185896416
500 500 a, t dbSNP:142907528
505 505 a, c dbSNP:776328834
515 515 -, c dbSNP:746615680
518 518 a, g dbSNP:759119506
526 526 c, g dbSNP:201167793
531 531 c, t dbSNP:75795289
538 538 a, t dbSNP:750206115
539 539 a, c, g dbSNP:755208649
540 540 c, t dbSNP:753014113
544 544 c, t dbSNP:758535504
547 547 a, g dbSNP:566294180
549 549 c, t dbSNP:139133362
550 550 a, g, t dbSNP:60452492
555 555 c, t dbSNP:756169972
556 556 a, g dbSNP:56040284
557 557 c, t dbSNP:548262477
558 558 a, g dbSNP:749371010
559 559 c, t dbSNP:557994567
567 567 -, cgtcatag dbSNP:769163444
567 567 c, t dbSNP:568558058
568 568 a, g dbSNP:6413419
573 573 a, g dbSNP:201427783
576 576 c, g, t dbSNP:79549556
577 577 a, g dbSNP:76138620
589 589 c, t dbSNP:140500826
590 590 a, g dbSNP:200871390
601 601 a, g dbSNP:752959099
608 608 a, g dbSNP:763286285
615 615 g, t dbSNP:764189163
624 624 a, g dbSNP:746192147
630 630 a, g dbSNP:751624559
631 631 a, t dbSNP:757401077
645 645 c, t dbSNP:28371743
655 655 c, t dbSNP:750987941
657 657 c, g, t dbSNP:772559232
658 658 c, t dbSNP:780401304
660 660 a, g dbSNP:774993741
663 663 c, t dbSNP:368615861
666 666 c, t dbSNP:768213976
667 667 a, g dbSNP:778445040
669 669 g, t dbSNP:747578103
670 670 c, t dbSNP:577159401
678 678 c, t dbSNP:773265206
687 687 c, t dbSNP:776753572
688 688 a, g dbSNP:41299426
689 689 a, g dbSNP:78313785
692 692 a, c dbSNP:769691101
696 696 c, t dbSNP:774432744
699 699 a, c dbSNP:374547066
704 704 c, t dbSNP:767527110
709 709 c, t dbSNP:773301045
716 716 g, t dbSNP:761129818
721 721 a, g dbSNP:766838345
736 736 a, g dbSNP:754388548
739 739 a, t dbSNP:375484572
740 740 c, t dbSNP:569948279
741 741 a, t dbSNP:535347009
745 745 a, t dbSNP:752221593
757 757 a, g dbSNP:758094862
765 765 a, g dbSNP:369888957
768 768 -, tg dbSNP:767473413
768 768 a, t dbSNP:555344732
769 769 a, g dbSNP:756879881
780 780 g, t dbSNP:762739956
792 792 c, t dbSNP:200017188
793 793 c, t dbSNP:745886844
803 803 a, t dbSNP:769409483
807 807 c, t dbSNP:775370009
812 812 a, g dbSNP:748268978
816 816 a, t dbSNP:772311713
818 818 c, t dbSNP:540609626
820 820 c, t dbSNP:372834832
821 821 a, g dbSNP:766501004
826 826 c, t dbSNP:776997036
832 832 a, g dbSNP:201869253
843 843 c, t dbSNP:370454377
850 850 a, g dbSNP:753140012
853 853 c, g dbSNP:372404508
855 855 a, g dbSNP:758878010
872 872 c, t dbSNP:751192911
876 876 a, g dbSNP:374287303
877 877 c, t dbSNP:375149895
878 878 a, g dbSNP:749979006
886 886 a, g dbSNP:138201091
888 888 a, g dbSNP:780017354
894 894 c, t dbSNP:527959204
895 895 a, g dbSNP:61710826
902 902 c, t dbSNP:778681344
903 903 c, t dbSNP:149170969
904 904 a, g dbSNP:771160948
906 906 a, g dbSNP:781313031
914 914 c, t dbSNP:745942247
915 915 c, t dbSNP:367879853
918 918 c, t dbSNP:201925769
924 924 c, t dbSNP:143114791
929 929 c, t dbSNP:533468318
930 930 c, g dbSNP:774878128
931 931 a, g dbSNP:761515989
933 933 c, g dbSNP:34767794
934 934 a, t dbSNP:750089893
941 941 a, c dbSNP:201120875
942 942 a, c dbSNP:28371746
944 944 c, g dbSNP:765869376
947 947 a, g dbSNP:753706527
951 951 a, c dbSNP:372112053
959 959 g, t dbSNP:764958966
966 966 g, t dbSNP:757319974
967 967 c, t dbSNP:374740281
974 974 g, t dbSNP:142845267
978 978 c, t dbSNP:781465269
979 979 a, c dbSNP:746145983
985 985 c, t dbSNP:756153015
996 996 c, t dbSNP:915909
997 997 a, g dbSNP:138223492
1003 1003 a, g dbSNP:773459833
1006 1006 c, g dbSNP:746620834
1019 1019 c, t dbSNP:770517759
1026 1026 a, g dbSNP:376820271
1029 1029 a, g dbSNP:759036748
1031 1031 c, t dbSNP:764799317
1032 1032 g, t dbSNP:775343042
1036 1036 c, g dbSNP:762813109
1042 1042 c, t dbSNP:200228968
1043 1043 a, g, t dbSNP:759707874
1047 1047 c, t dbSNP:761709128
1050 1050 c, t dbSNP:768112863
1056 1056 c, g dbSNP:61644766
1057 1057 a, g dbSNP:754169072
1074 1074 -, gccctac dbSNP:760975752
1077 1077 c, g, t dbSNP:146344065
1079 1079 a, g dbSNP:753186824
1082 1082 g, t dbSNP:150514905
1092 1092 a, g dbSNP:778157014
1093 1093 c, g dbSNP:747302212
1095 1095 a, g dbSNP:771346668
1108 1108 c, t dbSNP:780820318
1109 1109 a, g dbSNP:745528149
1114 1114 a, t dbSNP:769239142
1118 1118 c, t dbSNP:372279263
1123 1123 a, g dbSNP:762908601
1130 1130 c, g dbSNP:41299434
1139 1139 c, t dbSNP:768448978
1150 1150 a, g dbSNP:59981143
1152 1152 c, t dbSNP:200439431
1153 1153 c, g dbSNP:370530375
1154 1154 a, g dbSNP:767486762
1157 1157 a, g dbSNP:754046416
1158 1158 c, g dbSNP:759847516
1167 1167 a, c dbSNP:765355752
1172 1172 g, t dbSNP:752748963
1173 1173 a, c, g dbSNP:567473242
1182 1182 c, g, t dbSNP:752179109
1185 1185 c, t dbSNP:781553708
1187 1187 a, g dbSNP:199856651
1197 1197 c, t dbSNP:756237171
1198 1198 a, g, t dbSNP:55897648
1210 1210 c, g dbSNP:757788272
1214 1214 a, g dbSNP:60207639
1219 1219 c, g dbSNP:59656378
1232 1232 a, g dbSNP:756521777
1235 1235 a, c dbSNP:779561569
1248 1248 a, t dbSNP:749034745
1249 1249 c, g dbSNP:200177135
1271 1271 a, g dbSNP:369048851
1275 1275 c, t dbSNP:754377193
1286 1286 a, g dbSNP:57702102
1292 1292 a, t dbSNP:748046628
1296 1296 c, t dbSNP:2515641
1299 1299 a, g dbSNP:773320312
1303 1303 a, g dbSNP:142767992
1318 1318 c, g dbSNP:151041343
1326 1326 c, t dbSNP:775511413
1340 1340 c, t dbSNP:761257042
1341 1341 a, g dbSNP:766603842
1347 1347 a, t dbSNP:555961338
1357 1357 c, t dbSNP:779267176
1361 1361 a, c dbSNP:754256199
1363 1363 c, t dbSNP:140969399
1364 1364 a, c, g, t dbSNP:764917641
1368 1368 c, g dbSNP:199855848
1381 1381 c, t dbSNP:751497564
1386 1386 a, g dbSNP:534578330
1388 1388 c, g dbSNP:746030618
1391 1391 c, t dbSNP:369066427
1403 1403 a, t dbSNP:28969387
1422 1422 c, t dbSNP:149793864
1423 1423 a, g dbSNP:187941410
1427 1427 a, t dbSNP:769536455
1429 1429 c, t dbSNP:779104532
1430 1430 c, t dbSNP:748372752
1440 1440 c, t dbSNP:371128346
1441 1441 a, g, t dbSNP:199954662
1444 1444 c, t dbSNP:771433748
1484 1484 a, g dbSNP:55982231
1489 1489 a, g dbSNP:750408412
1495 1495 -, tg dbSNP:766643672
1507 1507 c, t dbSNP:759928543
1508 1508 a, g dbSNP:147916524
1511 1511 c, t dbSNP:752341899
1516 1516 c, g dbSNP:762720959
1520 1520 a, g dbSNP:763661003
1522 1522 -, gga dbSNP:753701630
1523 1523 a, g dbSNP:751053110
1527 1527 a, c dbSNP:756766217
1529 1529 a, c dbSNP:781176213
1532 1532 c, g dbSNP:750396234
1534 1534 a, g dbSNP:374012342
1536 1536 -, c dbSNP:754774326
1537 1537 c, g, t dbSNP:7081484
1538 1538 c, t dbSNP:748319538
1539 1539 c, t dbSNP:772311577
1542 1542 c, g, t dbSNP:370566113
1543 1543 a, g, t dbSNP:770971237
1545 1545 g, t dbSNP:746378183
1556 1556 a, c, t dbSNP:2480257
1560 1560 a, c dbSNP:56307258
1561 1561 a, g dbSNP:2480256
1567 1567 c, t dbSNP:11101812
1601 1601 a, t dbSNP:535324689
1608 1608 a, g dbSNP:762162892
1647 1647 a, c dbSNP:12164704

Target ORF information:

RefSeq Version NM_000773
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000773

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCTGCCC TCGGAGTCAC CGTGGCCCTG CTGGTGTGGG CGGCCTTCCT CCTGCTGGTG 
TCCATGTGGA GGCAGGTGCA CAGCAGCTGG AATCTGCCCC CAGGCCCTTT CCCGCTTCCC
ATCATCGGGA ACCTCTTCCA GTTGGAATTG AAGAATATTC CCAAGTCCTT CACCCGGTTG
GCCCAGCGCT TCGGGCCGGT GTTCACGCTG TACGTGGGCT CGCAGCGCAT GGTGGTGATG
CACGGCTACA AGGCGGTGAA GGAAGCGCTG CTGGACTACA AGGACGAGTT CTCGGGCAGA
GGCGACCTCC CCGCGTTCCA TGCGCACAGG GACAGGGGAA TCATTTTTAA TAATGGACCT
ACCTGGAAGG ACATCCGGCG GTTTTCCCTG ACCACCCTCC GGAACTATGG GATGGGGAAA
CAGGGCAATG AGAGCCGGAT CCAGAGGGAG GCCCACTTCC TGCTGGAAGC ACTCAGGAAG
ACCCAAGGCC AGCCTTTCGA CCCCACCTTC CTCATCGGCT GCGCGCCCTG CAACGTCATA
GCCGACATCC TCTTCCGCAA GCATTTTGAC TACAATGATG AGAAGTTTCT AAGGCTGATG
TATTTGTTTA ATGAGAACTT CCACCTACTC AGCACTCCCT GGCTCCAGCT TTACAATAAT
TTTCCCAGCT TTCTACACTA CTTGCCTGGA AGCCACAGAA AAGTCATAAA AAATGTGGCT
GAAGTAAAAG AGTATGTGTC TGAAAGGGTG AAGGAGCACC ATCAATCTCT GGACCCCAAC
TGTCCCCGGG ACCTCACCGA CTGCCTGCTC GTGGAAATGG AGAAGGAAAA GCACAGTGCA
GAGCGCTTGT ACACAATGGA CGGTATCACC GTGACTGTGG CCGACCTGTT CTTTGCGGGG
ACAGAGACCA CCAGCACAAC TCTGAGATAT GGGCTCCTGA TTCTCATGAA ATACCCTGAG
ATCGAAGAGA AGCTCCATGA AGAAATTGAC AGGGTGATTG GGCCAAGCCG AATCCCTGCC
ATCAAGGATA GGCAAGAGAT GCCCTACATG GATGCTGTGG TGCATGAGAT TCAGCGGTTC
ATCACCCTCG TGCCCTCCAA CCTGCCCCAT GAAGCAACCC GAGACACCAT TTTCAGAGGA
TACCTCATCC CCAAGGGCAC AGTCGTAGTG CCAACTCTGG ACTCTGTTTT GTATGACAAC
CAAGAATTTC CTGATCCAGA AAAGTTTAAG CCAGAACACT TCCTGAATGA AAATGGAAAG
TTCAAGTACA GTGACTATTT CAAGCCATTT TCCACAGGAA AACGAGTGTG TGCTGGAGAA
GGCCTGGCTC GCATGGAGTT GTTTCTTTTG TTGTGTGCCA TTTTGCAGCA TTTTAATTTG
AAGCCTCTCG TTGACCCAAA GGATATCGAC CTCAGCCCTA TACATATTGG GTTTGGCTGT
ATCCCACCAC GTTACAAACT CTGTGTCATT CCCCGCTCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.