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ADSL adenylosuccinate lyase [Homo sapiens (human)]

Gene Symbol ADSL
Entrez Gene ID 158
Full Name adenylosuccinate lyase
Synonyms AMPS, ASASE, ASL
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q13.2

Summary Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

608222

Disorder Html: Adenylosuccinase deficiency, 103050 (3)





The following ADSL gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADSL gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56197 XM_011529976 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu56198 XM_011529977 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu56199 XM_011529978 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu56200 XM_011529979 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu56201 XM_011529980 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu56202 XM_011529981 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu56203 XM_011529982 PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu18183 NM_000026 Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $379
OHu17522 NM_001123378 Homo sapiens adenylosuccinate lyase (ADSL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $359

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu56197
Accession Version XM_011529976.1
Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GATAGCCTGT
TATTCAGCTG GTGACGTCAG TAATTTTCCA CAGCAGCCAT TCATGAGCTC ATGCAGACTG
TCACAAGGAA ATGGGAATAG CACAATGAGT GTTACAGAAG AAAAAAAGAT CCTGTTTCTT
TGCCATCACA TATTATACAC AGACTTAGGG AATCTAGACT GGAGCACAAG ATATGTGGGA
GGGGTTAGAA CACCACTCCT ACCTGTAGTA ATAATCATTG AAACCACTTA TTGGATGCCT
TCTGCTTACC AGACACTAGT TGTCACAGGT CACTGTAAGT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528278.1
CDS60..1781
Misc Feature(1)108..1439(+)
Misc Feature(2)108..1415(+)
Misc Feature(3)150..1415(+)
Misc Feature(4)315..965(+)
Translation
Position Chain Variation Link
16 16 c, g dbSNP:763900902
20 20 c, t dbSNP:753745335
23 23 c, g, t dbSNP:372357778
24 24 a, t dbSNP:544434425
25 25 a, g, t dbSNP:750649096
27 27 g, t dbSNP:780488636
29 29 c, t dbSNP:747293932
36 36 c, t dbSNP:755335917
37 37 c, t dbSNP:201625622
38 38 c, g, t dbSNP:746438478
39 39 a, t dbSNP:183190870
42 42 c, t dbSNP:745531733
45 45 a, g dbSNP:771578438
48 48 c, t dbSNP:775388314
49 49 g, t dbSNP:200713871
51 51 a, g dbSNP:763926844
52 52 g, t dbSNP:560389412
54 54 a, g dbSNP:761671521
55 55 g, t dbSNP:765313611
64 64 c, t dbSNP:143083947
68 68 c, t dbSNP:758676257
70 70 g, t dbSNP:766546584
74 74 a, c, t dbSNP:751781160
75 75 c, g, t dbSNP:140064577
77 77 a, t dbSNP:756636478
78 78 c, g dbSNP:778590882
79 79 a, g dbSNP:377248090
81 81 c, g dbSNP:143916630
82 82 c, g dbSNP:540597292
86 86 g, t dbSNP:146873132
87 87 c, t dbSNP:768340455
89 89 a, c dbSNP:776352156
90 90 g, t dbSNP:761759515
91 91 a, t dbSNP:368501116
92 92 c, g dbSNP:8192454
95 95 a, c dbSNP:773098808
98 98 c, t dbSNP:762989462
99 99 c, t dbSNP:766465184
100 100 a, g, t dbSNP:371824098
101 101 c, t dbSNP:768010749
105 105 c, t dbSNP:753065207
106 106 c, t dbSNP:541051390
113 113 c, t dbSNP:756722245
124 124 c, t dbSNP:778466007
129 129 c, t dbSNP:754349909
132 132 g, t dbSNP:757904153
137 137 a, g dbSNP:779612414
149 149 c, t dbSNP:746654337
150 150 a, c dbSNP:768305498
151 151 a, g dbSNP:5757921
160 160 a, g dbSNP:780994144
172 172 c, t dbSNP:747976292
173 173 a, g dbSNP:377588571
182 182 a, g dbSNP:773188746
183 183 c, t dbSNP:2228415
188 188 c, g dbSNP:770916318
194 194 a, g dbSNP:774435749
199 199 c, g dbSNP:759704594
202 202 a, g dbSNP:767809983
205 205 c, g dbSNP:752938873
206 206 c, t dbSNP:761172321
207 207 c, g dbSNP:764542443
213 213 -, a dbSNP:765158535
220 220 a, g dbSNP:765813625
228 228 a, c, g dbSNP:750871310
232 232 c, t dbSNP:544172142
248 248 a, g dbSNP:767005918
257 257 a, c, g dbSNP:752349207
264 264 c, g dbSNP:200169254
272 272 c, t dbSNP:562234311
275 275 c, g, t dbSNP:145786986
276 276 a, g dbSNP:745787396
283 283 a, g dbSNP:772345709
285 285 a, c dbSNP:145306334
288 288 a, g dbSNP:775646855
290 290 a, c dbSNP:747255804
303 303 a, c dbSNP:768958353
304 304 a, g dbSNP:776685935
306 306 c, t dbSNP:780425464
307 307 a, g dbSNP:765503061
313 313 a, g dbSNP:540648461
314 314 a, g dbSNP:747492297
322 322 c, t dbSNP:149165656
329 329 c, t dbSNP:143275316
330 330 c, t dbSNP:755518176
332 332 c, t dbSNP:148303726
333 333 a, g dbSNP:763852823
341 341 a, g dbSNP:141408072
348 348 c, t dbSNP:757135310
357 357 c, g dbSNP:119450942
364 364 c, g dbSNP:369139561
366 366 g, t dbSNP:761359362
390 390 a, g dbSNP:758355156
395 395 c, t dbSNP:559415958
399 399 c, t dbSNP:374259530
401 401 c, t dbSNP:147017931
406 406 a, g dbSNP:768596079
410 410 c, t dbSNP:781534496
422 422 a, g dbSNP:138203195
436 436 a, g dbSNP:771267221
440 440 a, g dbSNP:774900669
443 443 c, t dbSNP:376894611
457 457 c, g, t dbSNP:768217316
461 461 g, t dbSNP:775948703
465 465 a, g dbSNP:773964967
467 467 c, t dbSNP:368166273
468 468 a, g dbSNP:767397881
473 473 c, g dbSNP:752659791
480 480 c, t dbSNP:756210458
481 481 a, g dbSNP:563054392
486 486 g, t dbSNP:764157818
488 488 c, t dbSNP:754065119
489 489 a, g dbSNP:757413931
497 497 a, t dbSNP:779272702
498 498 a, g dbSNP:746180694
499 499 a, t dbSNP:11089991
504 504 c, g dbSNP:780482118
505 505 a, g dbSNP:747546394
510 510 a, g dbSNP:147194356
524 524 a, g dbSNP:139543370
539 539 c, t dbSNP:149276957
541 541 a, g dbSNP:8192457
544 544 c, g dbSNP:745533324
550 550 a, g dbSNP:775392568
560 560 a, g dbSNP:771772590
570 570 c, t dbSNP:775298300
571 571 a, g dbSNP:760629234
587 587 c, t dbSNP:534209963
600 600 a, g dbSNP:768542145
604 604 a, g dbSNP:776521574
621 621 a, c dbSNP:371892194
622 622 a, g dbSNP:761937276
626 626 c, t dbSNP:765353500
627 627 c, t dbSNP:750614500
628 628 a, g dbSNP:28941471
635 635 a, c dbSNP:754005223
638 638 c, g dbSNP:751928831
640 640 a, g dbSNP:755359802
645 645 c, t dbSNP:376533106
646 646 a, g, t dbSNP:753245184
651 651 a, g dbSNP:778422871
670 670 c, t dbSNP:745524617
675 675 a, g, t dbSNP:148411623
676 676 c, g dbSNP:746848060
679 679 c, g dbSNP:369400468
682 682 a, t dbSNP:776615568
690 690 c, t dbSNP:373777376
698 698 g, t dbSNP:376751756
708 708 c, g dbSNP:199761158
733 733 c, t dbSNP:119450945
760 760 g, t dbSNP:748105288
778 778 c, g dbSNP:754574998
779 779 a, g dbSNP:780996092
793 793 a, g dbSNP:374047157
794 794 a, t dbSNP:143977255
795 795 a, g dbSNP:119450944
810 810 -, gtactgtct dbSNP:769288234
817 817 a, c dbSNP:777822535
819 819 g, t dbSNP:749180603
822 822 c, t dbSNP:771049726
828 828 a, g dbSNP:775115015
829 829 a, g dbSNP:746114979
833 833 g, t dbSNP:78915065
848 848 c, t dbSNP:772388333
859 859 a, c dbSNP:367719924
860 860 c, t dbSNP:369069575
861 861 a, g dbSNP:746501563
864 864 a, g dbSNP:759104126
865 865 -, a dbSNP:774729848
866 866 a, g dbSNP:767008067
867 867 c, t dbSNP:181252862
868 868 a, g dbSNP:760189192
890 890 c, g dbSNP:763973471
893 893 a, g, t dbSNP:2735668
901 901 c, g dbSNP:371529148
906 906 c, g dbSNP:779046571
915 915 c, g dbSNP:376912453
922 922 c, g dbSNP:776328750
929 929 c, t dbSNP:146890981
932 932 a, g dbSNP:765122847
933 933 a, g dbSNP:750393308
938 938 a, g dbSNP:758359453
939 939 c, t dbSNP:8192461
945 945 c, t dbSNP:536254357
946 946 a, g dbSNP:755059672
953 953 c, t dbSNP:145469983
954 954 a, g dbSNP:34396910
957 957 a, c dbSNP:369617680
966 966 c, t dbSNP:373458753
984 984 c, t dbSNP:376357524
985 985 a, g dbSNP:749817666
1012 1012 c, t dbSNP:202064195
1013 1013 a, g dbSNP:774891125
1044 1044 c, t dbSNP:370221257
1045 1045 a, g dbSNP:768203123
1053 1053 c, g dbSNP:776496275
1062 1062 g, t dbSNP:79075278
1068 1068 c, t dbSNP:761493155
1071 1071 c, t dbSNP:769607090
1075 1075 a, t dbSNP:772974251
1076 1076 c, g dbSNP:762710500
1081 1081 c, t dbSNP:770755205
1086 1086 a, g dbSNP:774159147
1088 1088 a, g dbSNP:759459022
1100 1100 c, t dbSNP:767608764
1101 1101 a, g dbSNP:752735865
1112 1112 a, g dbSNP:151095874
1115 1115 a, g dbSNP:764349846
1119 1119 a, g dbSNP:75953451
1120 1120 c, t dbSNP:752238667
1121 1121 a, g dbSNP:757634112
1127 1127 a, g dbSNP:11703124
1131 1131 a, g dbSNP:779273839
1146 1146 a, g dbSNP:375375168
1147 1147 c, t dbSNP:758895348
1148 1148 c, t dbSNP:368373129
1149 1149 a, g dbSNP:370851726
1153 1153 a, g dbSNP:769412149
1164 1164 a, g dbSNP:752104617
1165 1165 c, t dbSNP:755688868
1167 1167 a, g dbSNP:267606256
1170 1170 a, c, g dbSNP:777359946
1171 1171 a, g dbSNP:192303222
1173 1173 a, c, t dbSNP:778401578
1174 1174 a, g dbSNP:150228971
1176 1176 a, c dbSNP:775475956
1179 1179 c, t dbSNP:376533026
1180 1180 a, g dbSNP:568567422
1190 1190 a, g dbSNP:776727558
1194 1194 g, t dbSNP:761764731
1197 1197 a, g dbSNP:368160421
1206 1206 a, g dbSNP:765543265
1215 1215 a, g dbSNP:773289988
1224 1224 a, g dbSNP:763475264
1226 1226 a, g dbSNP:766782678
1241 1241 c, t dbSNP:752125280
1245 1245 c, t dbSNP:755492501
1246 1246 a, g dbSNP:763542069
1248 1248 c, t dbSNP:753430039
1253 1253 c, t dbSNP:779859012
1259 1259 c, t dbSNP:751401941
1266 1266 a, c dbSNP:754714101
1269 1269 -, ag dbSNP:773567586
1271 1271 a, c dbSNP:781253590
1281 1281 c, t dbSNP:747964752
1288 1288 c, t dbSNP:542937686
1294 1294 a, c, t dbSNP:571597579
1303 1303 -, ag dbSNP:760899856
1307 1307 a, g dbSNP:771806949
1311 1311 c, g dbSNP:372650859
1312 1312 a, g dbSNP:774693972
1320 1320 -, aat dbSNP:778107338
1321 1321 a, g dbSNP:145750778
1323 1323 g, t dbSNP:119450943
1329 1329 a, g dbSNP:776070877
1336 1336 a, g dbSNP:119450941
1337 1337 c, t dbSNP:764829606
1338 1338 a, g dbSNP:758241731
1340 1340 c, g dbSNP:762422248
1345 1345 c, t dbSNP:372051078
1347 1347 a, g dbSNP:554254383
1350 1350 a, g dbSNP:200814886
1354 1354 a, c dbSNP:754803189
1364 1364 c, t dbSNP:780788761
1367 1367 g, t dbSNP:61755965
1371 1371 c, t dbSNP:119450940
1381 1381 a, t dbSNP:752702384
1396 1396 -, ctt dbSNP:766428638
1396 1396 a, c dbSNP:755964863
1398 1398 c, t dbSNP:777821034
1399 1399 c, t dbSNP:749427506
1401 1401 c, t dbSNP:771121666
1405 1405 c, t dbSNP:368990043
1408 1408 c, g, t dbSNP:372895468
1409 1409 c, t dbSNP:200300396
1411 1411 a, g dbSNP:377120302
1413 1413 a, c, g dbSNP:572438339
1414 1414 a, g dbSNP:775671027
1427 1427 -, g dbSNP:752585403
1428 1428 a, g dbSNP:747385959
1464 1464 g, t dbSNP:768999974
1475 1475 c, t dbSNP:777123162
1481 1481 c, t dbSNP:748593655
1485 1485 a, g dbSNP:377758918
1487 1487 a, g dbSNP:773810168
1605 1605 a, t dbSNP:376076399
1620 1620 c, t dbSNP:547923048
1624 1624 a, g dbSNP:180968801
1632 1632 c, t dbSNP:117688975
1638 1638 a, t dbSNP:551772911
1639 1639 -, aa dbSNP:769111521
1681 1681 a, g dbSNP:186424917
1757 1757 a, g dbSNP:137899556
1777 1777 a, g dbSNP:367915009
1801 1801 a, t dbSNP:555061450
1834 1834 c, t dbSNP:573364205
1869 1869 c, t dbSNP:534523199

Target ORF information:

RefSeq Version XM_011529976
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529976

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GATAGCCTGT
TATTCAGCTG GTGACGTCAG TAATTTTCCA CAGCAGCCAT TCATGAGCTC ATGCAGACTG
TCACAAGGAA ATGGGAATAG CACAATGAGT GTTACAGAAG AAAAAAAGAT CCTGTTTCTT
TGCCATCACA TATTATACAC AGACTTAGGG AATCTAGACT GGAGCACAAG ATATGTGGGA
GGGGTTAGAA CACCACTCCT ACCTGTAGTA ATAATCATTG AAACCACTTA TTGGATGCCT
TCTGCTTACC AGACACTAGT TGTCACAGGT CACTGTAAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56198
Accession Version XM_011529977.1
Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GTGTAAGCAA
GTGCCATTGG ACAAGAACGT TGTCTGTGTC ATTCCTTGTT GTATTTCCAA TAGCAAGAGC
ACATCAGATA CTTGGCAACT AGGCAGTCTT CCAGCTTCTG CCACTGCGCC CGACTTCCTT
GTTGCATGGA TTTATAGCTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528279.1
CDS60..1640
Misc Feature(1)108..1439(+)
Misc Feature(2)108..1415(+)
Misc Feature(3)150..1415(+)
Misc Feature(4)315..965(+)
Translation
Position Chain Variation Link
16 16 c, g dbSNP:763900902
20 20 c, t dbSNP:753745335
23 23 c, g, t dbSNP:372357778
24 24 a, t dbSNP:544434425
25 25 a, g, t dbSNP:750649096
27 27 g, t dbSNP:780488636
29 29 c, t dbSNP:747293932
36 36 c, t dbSNP:755335917
37 37 c, t dbSNP:201625622
38 38 c, g, t dbSNP:746438478
39 39 a, t dbSNP:183190870
42 42 c, t dbSNP:745531733
45 45 a, g dbSNP:771578438
48 48 c, t dbSNP:775388314
49 49 g, t dbSNP:200713871
51 51 a, g dbSNP:763926844
52 52 g, t dbSNP:560389412
54 54 a, g dbSNP:761671521
55 55 g, t dbSNP:765313611
64 64 c, t dbSNP:143083947
68 68 c, t dbSNP:758676257
70 70 g, t dbSNP:766546584
74 74 a, c, t dbSNP:751781160
75 75 c, g, t dbSNP:140064577
77 77 a, t dbSNP:756636478
78 78 c, g dbSNP:778590882
79 79 a, g dbSNP:377248090
81 81 c, g dbSNP:143916630
82 82 c, g dbSNP:540597292
86 86 g, t dbSNP:146873132
87 87 c, t dbSNP:768340455
89 89 a, c dbSNP:776352156
90 90 g, t dbSNP:761759515
91 91 a, t dbSNP:368501116
92 92 c, g dbSNP:8192454
95 95 a, c dbSNP:773098808
98 98 c, t dbSNP:762989462
99 99 c, t dbSNP:766465184
100 100 a, g, t dbSNP:371824098
101 101 c, t dbSNP:768010749
105 105 c, t dbSNP:753065207
106 106 c, t dbSNP:541051390
113 113 c, t dbSNP:756722245
124 124 c, t dbSNP:778466007
129 129 c, t dbSNP:754349909
132 132 g, t dbSNP:757904153
137 137 a, g dbSNP:779612414
149 149 c, t dbSNP:746654337
150 150 a, c dbSNP:768305498
151 151 a, g dbSNP:5757921
160 160 a, g dbSNP:780994144
172 172 c, t dbSNP:747976292
173 173 a, g dbSNP:377588571
182 182 a, g dbSNP:773188746
183 183 c, t dbSNP:2228415
188 188 c, g dbSNP:770916318
194 194 a, g dbSNP:774435749
199 199 c, g dbSNP:759704594
202 202 a, g dbSNP:767809983
205 205 c, g dbSNP:752938873
206 206 c, t dbSNP:761172321
207 207 c, g dbSNP:764542443
213 213 -, a dbSNP:765158535
220 220 a, g dbSNP:765813625
228 228 a, c, g dbSNP:750871310
232 232 c, t dbSNP:544172142
248 248 a, g dbSNP:767005918
257 257 a, c, g dbSNP:752349207
264 264 c, g dbSNP:200169254
272 272 c, t dbSNP:562234311
275 275 c, g, t dbSNP:145786986
276 276 a, g dbSNP:745787396
283 283 a, g dbSNP:772345709
285 285 a, c dbSNP:145306334
288 288 a, g dbSNP:775646855
290 290 a, c dbSNP:747255804
303 303 a, c dbSNP:768958353
304 304 a, g dbSNP:776685935
306 306 c, t dbSNP:780425464
307 307 a, g dbSNP:765503061
313 313 a, g dbSNP:540648461
314 314 a, g dbSNP:747492297
322 322 c, t dbSNP:149165656
329 329 c, t dbSNP:143275316
330 330 c, t dbSNP:755518176
332 332 c, t dbSNP:148303726
333 333 a, g dbSNP:763852823
341 341 a, g dbSNP:141408072
348 348 c, t dbSNP:757135310
357 357 c, g dbSNP:119450942
364 364 c, g dbSNP:369139561
366 366 g, t dbSNP:761359362
390 390 a, g dbSNP:758355156
395 395 c, t dbSNP:559415958
399 399 c, t dbSNP:374259530
401 401 c, t dbSNP:147017931
406 406 a, g dbSNP:768596079
410 410 c, t dbSNP:781534496
422 422 a, g dbSNP:138203195
436 436 a, g dbSNP:771267221
440 440 a, g dbSNP:774900669
443 443 c, t dbSNP:376894611
457 457 c, g, t dbSNP:768217316
461 461 g, t dbSNP:775948703
465 465 a, g dbSNP:773964967
467 467 c, t dbSNP:368166273
468 468 a, g dbSNP:767397881
473 473 c, g dbSNP:752659791
480 480 c, t dbSNP:756210458
481 481 a, g dbSNP:563054392
486 486 g, t dbSNP:764157818
488 488 c, t dbSNP:754065119
489 489 a, g dbSNP:757413931
497 497 a, t dbSNP:779272702
498 498 a, g dbSNP:746180694
499 499 a, t dbSNP:11089991
504 504 c, g dbSNP:780482118
505 505 a, g dbSNP:747546394
510 510 a, g dbSNP:147194356
524 524 a, g dbSNP:139543370
539 539 c, t dbSNP:149276957
541 541 a, g dbSNP:8192457
544 544 c, g dbSNP:745533324
550 550 a, g dbSNP:775392568
560 560 a, g dbSNP:771772590
570 570 c, t dbSNP:775298300
571 571 a, g dbSNP:760629234
587 587 c, t dbSNP:534209963
600 600 a, g dbSNP:768542145
604 604 a, g dbSNP:776521574
621 621 a, c dbSNP:371892194
622 622 a, g dbSNP:761937276
626 626 c, t dbSNP:765353500
627 627 c, t dbSNP:750614500
628 628 a, g dbSNP:28941471
635 635 a, c dbSNP:754005223
638 638 c, g dbSNP:751928831
640 640 a, g dbSNP:755359802
645 645 c, t dbSNP:376533106
646 646 a, g, t dbSNP:753245184
651 651 a, g dbSNP:778422871
670 670 c, t dbSNP:745524617
675 675 a, g, t dbSNP:148411623
676 676 c, g dbSNP:746848060
679 679 c, g dbSNP:369400468
682 682 a, t dbSNP:776615568
690 690 c, t dbSNP:373777376
698 698 g, t dbSNP:376751756
708 708 c, g dbSNP:199761158
733 733 c, t dbSNP:119450945
760 760 g, t dbSNP:748105288
778 778 c, g dbSNP:754574998
779 779 a, g dbSNP:780996092
793 793 a, g dbSNP:374047157
794 794 a, t dbSNP:143977255
795 795 a, g dbSNP:119450944
810 810 -, gtactgtct dbSNP:769288234
817 817 a, c dbSNP:777822535
819 819 g, t dbSNP:749180603
822 822 c, t dbSNP:771049726
828 828 a, g dbSNP:775115015
829 829 a, g dbSNP:746114979
833 833 g, t dbSNP:78915065
848 848 c, t dbSNP:772388333
859 859 a, c dbSNP:367719924
860 860 c, t dbSNP:369069575
861 861 a, g dbSNP:746501563
864 864 a, g dbSNP:759104126
865 865 -, a dbSNP:774729848
866 866 a, g dbSNP:767008067
867 867 c, t dbSNP:181252862
868 868 a, g dbSNP:760189192
890 890 c, g dbSNP:763973471
893 893 a, g, t dbSNP:2735668
901 901 c, g dbSNP:371529148
906 906 c, g dbSNP:779046571
915 915 c, g dbSNP:376912453
922 922 c, g dbSNP:776328750
929 929 c, t dbSNP:146890981
932 932 a, g dbSNP:765122847
933 933 a, g dbSNP:750393308
938 938 a, g dbSNP:758359453
939 939 c, t dbSNP:8192461
945 945 c, t dbSNP:536254357
946 946 a, g dbSNP:755059672
953 953 c, t dbSNP:145469983
954 954 a, g dbSNP:34396910
957 957 a, c dbSNP:369617680
966 966 c, t dbSNP:373458753
984 984 c, t dbSNP:376357524
985 985 a, g dbSNP:749817666
1012 1012 c, t dbSNP:202064195
1013 1013 a, g dbSNP:774891125
1044 1044 c, t dbSNP:370221257
1045 1045 a, g dbSNP:768203123
1053 1053 c, g dbSNP:776496275
1062 1062 g, t dbSNP:79075278
1068 1068 c, t dbSNP:761493155
1071 1071 c, t dbSNP:769607090
1075 1075 a, t dbSNP:772974251
1076 1076 c, g dbSNP:762710500
1081 1081 c, t dbSNP:770755205
1086 1086 a, g dbSNP:774159147
1088 1088 a, g dbSNP:759459022
1100 1100 c, t dbSNP:767608764
1101 1101 a, g dbSNP:752735865
1112 1112 a, g dbSNP:151095874
1115 1115 a, g dbSNP:764349846
1119 1119 a, g dbSNP:75953451
1120 1120 c, t dbSNP:752238667
1121 1121 a, g dbSNP:757634112
1127 1127 a, g dbSNP:11703124
1131 1131 a, g dbSNP:779273839
1146 1146 a, g dbSNP:375375168
1147 1147 c, t dbSNP:758895348
1148 1148 c, t dbSNP:368373129
1149 1149 a, g dbSNP:370851726
1153 1153 a, g dbSNP:769412149
1164 1164 a, g dbSNP:752104617
1165 1165 c, t dbSNP:755688868
1167 1167 a, g dbSNP:267606256
1170 1170 a, c, g dbSNP:777359946
1171 1171 a, g dbSNP:192303222
1173 1173 a, c, t dbSNP:778401578
1174 1174 a, g dbSNP:150228971
1176 1176 a, c dbSNP:775475956
1179 1179 c, t dbSNP:376533026
1180 1180 a, g dbSNP:568567422
1190 1190 a, g dbSNP:776727558
1194 1194 g, t dbSNP:761764731
1197 1197 a, g dbSNP:368160421
1206 1206 a, g dbSNP:765543265
1215 1215 a, g dbSNP:773289988
1224 1224 a, g dbSNP:763475264
1226 1226 a, g dbSNP:766782678
1241 1241 c, t dbSNP:752125280
1245 1245 c, t dbSNP:755492501
1246 1246 a, g dbSNP:763542069
1248 1248 c, t dbSNP:753430039
1253 1253 c, t dbSNP:779859012
1259 1259 c, t dbSNP:751401941
1266 1266 a, c dbSNP:754714101
1269 1269 -, ag dbSNP:773567586
1271 1271 a, c dbSNP:781253590
1281 1281 c, t dbSNP:747964752
1288 1288 c, t dbSNP:542937686
1294 1294 a, c, t dbSNP:571597579
1303 1303 -, ag dbSNP:760899856
1307 1307 a, g dbSNP:771806949
1311 1311 c, g dbSNP:372650859
1312 1312 a, g dbSNP:774693972
1320 1320 -, aat dbSNP:778107338
1321 1321 a, g dbSNP:145750778
1323 1323 g, t dbSNP:119450943
1329 1329 a, g dbSNP:776070877
1336 1336 a, g dbSNP:119450941
1337 1337 c, t dbSNP:764829606
1338 1338 a, g dbSNP:758241731
1340 1340 c, g dbSNP:762422248
1345 1345 c, t dbSNP:372051078
1347 1347 a, g dbSNP:554254383
1350 1350 a, g dbSNP:200814886
1354 1354 a, c dbSNP:754803189
1364 1364 c, t dbSNP:780788761
1367 1367 g, t dbSNP:61755965
1371 1371 c, t dbSNP:119450940
1381 1381 a, t dbSNP:752702384
1396 1396 -, ctt dbSNP:766428638
1396 1396 a, c dbSNP:755964863
1398 1398 c, t dbSNP:777821034
1399 1399 c, t dbSNP:749427506
1401 1401 c, t dbSNP:771121666
1405 1405 c, t dbSNP:368990043
1408 1408 c, g, t dbSNP:372895468
1409 1409 c, t dbSNP:200300396
1411 1411 a, g dbSNP:377120302
1413 1413 a, c, g dbSNP:572438339
1414 1414 a, g dbSNP:775671027
1427 1427 -, g dbSNP:752585403
1428 1428 a, g dbSNP:747385959
1464 1464 g, t dbSNP:768999974
1475 1475 c, t dbSNP:777123162
1481 1481 c, t dbSNP:748593655
1485 1485 a, g dbSNP:377758918
1487 1487 a, g dbSNP:773810168
1500 1500 a, g dbSNP:200511160
1586 1586 c, t dbSNP:536462179
1603 1603 c, g dbSNP:780529556
1606 1606 c, t dbSNP:747343285
1607 1607 a, g dbSNP:374233870
1615 1615 -, c dbSNP:34548480
1641 1641 c, t dbSNP:201506139
1642 1642 a, g, t dbSNP:199650962
1653 1653 g, t dbSNP:534687219
1673 1673 c, t dbSNP:530100881
1699 1699 a, g dbSNP:750305626
1717 1717 a, g dbSNP:146937362
1731 1731 c, t dbSNP:186087225
1732 1732 c, t dbSNP:762256631
1734 1734 g, t dbSNP:769341165
1802 1802 a, g dbSNP:76164361
1804 1804 a, g dbSNP:772564582
1820 1820 a, g dbSNP:577986408
1846 1846 a, g dbSNP:762732535
1863 1863 -, ctaaa dbSNP:553883483
1875 1875 -, a dbSNP:560833492
1896 1896 -, g dbSNP:781250384
1922 1922 a, g dbSNP:753360675
1934 1934 g, t dbSNP:545039108
1939 1939 a, g dbSNP:191857992
1946 1946 c, g dbSNP:111387329
1951 1951 -, g dbSNP:35202382
1983 1983 a, g dbSNP:137939016
1999 1999 c, t dbSNP:542706880
2005 2005 c, t dbSNP:181963971
2006 2006 a, g dbSNP:531516908
2013 2013 c, t dbSNP:528644746
2038 2038 c, t dbSNP:143509180
2069 2069 c, g dbSNP:565412931
2086 2086 c, g dbSNP:532150916
2088 2088 c, t dbSNP:751157422
2107 2107 a, g dbSNP:550269630
2120 2120 a, g dbSNP:759281875
2134 2134 c, t dbSNP:767374254
2139 2139 a, g dbSNP:562577095
2144 2144 g, t dbSNP:529861662
2169 2169 a, c dbSNP:779205808
2185 2185 a, g dbSNP:56082120
2190 2190 c, g, t dbSNP:186535129
2191 2191 c, g, t dbSNP:571388759
2245 2245 a, g dbSNP:533726923
2269 2269 c, t dbSNP:550026663
2273 2273 a, g dbSNP:55739141
2281 2281 c, t dbSNP:571561189
2290 2290 a, g dbSNP:780139835
2294 2294 a, g dbSNP:570612958
2348 2348 a, g dbSNP:539026232
2353 2353 -, ca dbSNP:745389202
2385 2385 c, t dbSNP:557122879
2454 2454 a, g dbSNP:748039605
2505 2505 -, aagag dbSNP:556730420
2524 2524 c, t dbSNP:147177295
2527 2527 a, g dbSNP:769028481
2560 2560 c, t dbSNP:575222704
2579 2579 c, t dbSNP:148139571
2592 2592 g, t dbSNP:748691891
2626 2626 a, g dbSNP:533758774
2628 2628 c, g dbSNP:6001888
2668 2668 a, g dbSNP:115756765
2674 2674 a, g dbSNP:190546028
2675 2675 g, t dbSNP:540439325
2686 2686 c, t dbSNP:566017381
2698 2698 c, t dbSNP:747253177

Target ORF information:

RefSeq Version XM_011529977
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529977

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GTGTAAGCAA
GTGCCATTGG ACAAGAACGT TGTCTGTGTC ATTCCTTGTT GTATTTCCAA TAGCAAGAGC
ACATCAGATA CTTGGCAACT AGGCAGTCTT CCAGCTTCTG CCACTGCGCC CGACTTCCTT
GTTGCATGGA TTTATAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56199
Accession Version XM_011529978.1
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGATAGCC
TGTTATTCAG CTGGTGACGT CAGTAATTTT CCACAGCAGC CATTCATGAG CTCATGCAGA
CTGTCACAAG GAAATGGGAA TAGCACAATG AGTGTTACAG AAGAAAAAAA GATCCTGTTT
CTTTGCCATC ACATATTATA CACAGACTTA GGGAATCTAG ACTGGAGCAC AAGATATGTG
GGAGGGGTTA GAACACCACT CCTACCTGTA GTAATAATCA TTGAAACCAC TTATTGGATG
CCTTCTGCTT ACCAGACACT AGTTGTCACA GGTCACTGTA AGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528280.1
CDS60..1604
Misc Feature(1)108..1349(+)
Misc Feature(2)108..1250(+)
Misc Feature(3)150..1250(+)
Misc Feature(4)315..965(+)
Translation
Position Chain Variation Link
16 16 c, g dbSNP:763900902
20 20 c, t dbSNP:753745335
23 23 c, g, t dbSNP:372357778
24 24 a, t dbSNP:544434425
25 25 a, g, t dbSNP:750649096
27 27 g, t dbSNP:780488636
29 29 c, t dbSNP:747293932
36 36 c, t dbSNP:755335917
37 37 c, t dbSNP:201625622
38 38 c, g, t dbSNP:746438478
39 39 a, t dbSNP:183190870
42 42 c, t dbSNP:745531733
45 45 a, g dbSNP:771578438
48 48 c, t dbSNP:775388314
49 49 g, t dbSNP:200713871
51 51 a, g dbSNP:763926844
52 52 g, t dbSNP:560389412
54 54 a, g dbSNP:761671521
55 55 g, t dbSNP:765313611
64 64 c, t dbSNP:143083947
68 68 c, t dbSNP:758676257
70 70 g, t dbSNP:766546584
74 74 a, c, t dbSNP:751781160
75 75 c, g, t dbSNP:140064577
77 77 a, t dbSNP:756636478
78 78 c, g dbSNP:778590882
79 79 a, g dbSNP:377248090
81 81 c, g dbSNP:143916630
82 82 c, g dbSNP:540597292
86 86 g, t dbSNP:146873132
87 87 c, t dbSNP:768340455
89 89 a, c dbSNP:776352156
90 90 g, t dbSNP:761759515
91 91 a, t dbSNP:368501116
92 92 c, g dbSNP:8192454
95 95 a, c dbSNP:773098808
98 98 c, t dbSNP:762989462
99 99 c, t dbSNP:766465184
100 100 a, g, t dbSNP:371824098
101 101 c, t dbSNP:768010749
105 105 c, t dbSNP:753065207
106 106 c, t dbSNP:541051390
113 113 c, t dbSNP:756722245
124 124 c, t dbSNP:778466007
129 129 c, t dbSNP:754349909
132 132 g, t dbSNP:757904153
137 137 a, g dbSNP:779612414
149 149 c, t dbSNP:746654337
150 150 a, c dbSNP:768305498
151 151 a, g dbSNP:5757921
160 160 a, g dbSNP:780994144
172 172 c, t dbSNP:747976292
173 173 a, g dbSNP:377588571
182 182 a, g dbSNP:773188746
183 183 c, t dbSNP:2228415
188 188 c, g dbSNP:770916318
194 194 a, g dbSNP:774435749
199 199 c, g dbSNP:759704594
202 202 a, g dbSNP:767809983
205 205 c, g dbSNP:752938873
206 206 c, t dbSNP:761172321
207 207 c, g dbSNP:764542443
213 213 -, a dbSNP:765158535
220 220 a, g dbSNP:765813625
228 228 a, c, g dbSNP:750871310
232 232 c, t dbSNP:544172142
248 248 a, g dbSNP:767005918
257 257 a, c, g dbSNP:752349207
264 264 c, g dbSNP:200169254
272 272 c, t dbSNP:562234311
275 275 c, g, t dbSNP:145786986
276 276 a, g dbSNP:745787396
283 283 a, g dbSNP:772345709
285 285 a, c dbSNP:145306334
288 288 a, g dbSNP:775646855
290 290 a, c dbSNP:747255804
303 303 a, c dbSNP:768958353
304 304 a, g dbSNP:776685935
306 306 c, t dbSNP:780425464
307 307 a, g dbSNP:765503061
313 313 a, g dbSNP:540648461
314 314 a, g dbSNP:747492297
322 322 c, t dbSNP:149165656
329 329 c, t dbSNP:143275316
330 330 c, t dbSNP:755518176
332 332 c, t dbSNP:148303726
333 333 a, g dbSNP:763852823
341 341 a, g dbSNP:141408072
348 348 c, t dbSNP:757135310
357 357 c, g dbSNP:119450942
364 364 c, g dbSNP:369139561
366 366 g, t dbSNP:761359362
390 390 a, g dbSNP:758355156
395 395 c, t dbSNP:559415958
399 399 c, t dbSNP:374259530
401 401 c, t dbSNP:147017931
406 406 a, g dbSNP:768596079
410 410 c, t dbSNP:781534496
422 422 a, g dbSNP:138203195
436 436 a, g dbSNP:771267221
440 440 a, g dbSNP:774900669
443 443 c, t dbSNP:376894611
457 457 c, g, t dbSNP:768217316
461 461 g, t dbSNP:775948703
465 465 a, g dbSNP:773964967
467 467 c, t dbSNP:368166273
468 468 a, g dbSNP:767397881
473 473 c, g dbSNP:752659791
480 480 c, t dbSNP:756210458
481 481 a, g dbSNP:563054392
486 486 g, t dbSNP:764157818
488 488 c, t dbSNP:754065119
489 489 a, g dbSNP:757413931
497 497 a, t dbSNP:779272702
498 498 a, g dbSNP:746180694
499 499 a, t dbSNP:11089991
504 504 c, g dbSNP:780482118
505 505 a, g dbSNP:747546394
510 510 a, g dbSNP:147194356
524 524 a, g dbSNP:139543370
539 539 c, t dbSNP:149276957
541 541 a, g dbSNP:8192457
544 544 c, g dbSNP:745533324
550 550 a, g dbSNP:775392568
560 560 a, g dbSNP:771772590
570 570 c, t dbSNP:775298300
571 571 a, g dbSNP:760629234
587 587 c, t dbSNP:534209963
600 600 a, g dbSNP:768542145
604 604 a, g dbSNP:776521574
621 621 a, c dbSNP:371892194
622 622 a, g dbSNP:761937276
626 626 c, t dbSNP:765353500
627 627 c, t dbSNP:750614500
628 628 a, g dbSNP:28941471
635 635 a, c dbSNP:754005223
638 638 c, g dbSNP:751928831
640 640 a, g dbSNP:755359802
645 645 c, t dbSNP:376533106
646 646 a, g, t dbSNP:753245184
651 651 a, g dbSNP:778422871
670 670 c, t dbSNP:745524617
675 675 a, g, t dbSNP:148411623
676 676 c, g dbSNP:746848060
679 679 c, g dbSNP:369400468
682 682 a, t dbSNP:776615568
690 690 c, t dbSNP:373777376
698 698 g, t dbSNP:376751756
708 708 c, g dbSNP:199761158
733 733 c, t dbSNP:119450945
760 760 g, t dbSNP:748105288
778 778 c, g dbSNP:754574998
779 779 a, g dbSNP:780996092
793 793 a, g dbSNP:374047157
794 794 a, t dbSNP:143977255
795 795 a, g dbSNP:119450944
810 810 -, gtactgtct dbSNP:769288234
817 817 a, c dbSNP:777822535
819 819 g, t dbSNP:749180603
822 822 c, t dbSNP:771049726
828 828 a, g dbSNP:775115015
829 829 a, g dbSNP:746114979
833 833 g, t dbSNP:78915065
848 848 c, t dbSNP:772388333
859 859 a, c dbSNP:367719924
860 860 c, t dbSNP:369069575
861 861 a, g dbSNP:746501563
864 864 a, g dbSNP:759104126
865 865 -, a dbSNP:774729848
866 866 a, g dbSNP:767008067
867 867 c, t dbSNP:181252862
868 868 a, g dbSNP:760189192
890 890 c, g dbSNP:763973471
893 893 a, g, t dbSNP:2735668
901 901 c, g dbSNP:371529148
906 906 c, g dbSNP:779046571
915 915 c, g dbSNP:376912453
922 922 c, g dbSNP:776328750
929 929 c, t dbSNP:146890981
932 932 a, g dbSNP:765122847
933 933 a, g dbSNP:750393308
938 938 a, g dbSNP:758359453
939 939 c, t dbSNP:8192461
945 945 c, t dbSNP:536254357
946 946 a, g dbSNP:755059672
953 953 c, t dbSNP:145469983
954 954 a, g dbSNP:34396910
957 957 a, c dbSNP:369617680
966 966 c, t dbSNP:373458753
984 984 c, t dbSNP:376357524
985 985 a, g dbSNP:749817666
1012 1012 c, t dbSNP:202064195
1013 1013 a, g dbSNP:774891125
1044 1044 c, t dbSNP:370221257
1045 1045 a, g dbSNP:768203123
1053 1053 c, g dbSNP:776496275
1062 1062 g, t dbSNP:79075278
1068 1068 c, t dbSNP:761493155
1071 1071 c, t dbSNP:769607090
1075 1075 a, t dbSNP:772974251
1076 1076 c, g dbSNP:762710500
1081 1081 c, t dbSNP:770755205
1086 1086 a, g dbSNP:774159147
1088 1088 a, g dbSNP:759459022
1100 1100 c, t dbSNP:767608764
1101 1101 a, g dbSNP:752735865
1112 1112 a, g dbSNP:151095874
1115 1115 a, g dbSNP:764349846
1119 1119 a, g dbSNP:75953451
1120 1120 c, t dbSNP:752238667
1121 1121 a, g dbSNP:757634112
1127 1127 a, g dbSNP:11703124
1131 1131 a, g dbSNP:779273839
1146 1146 a, g dbSNP:375375168
1147 1147 c, t dbSNP:758895348
1148 1148 c, t dbSNP:368373129
1149 1149 a, g dbSNP:370851726
1153 1153 a, g dbSNP:769412149
1164 1164 a, g dbSNP:752104617
1165 1165 c, t dbSNP:755688868
1167 1167 a, g dbSNP:267606256
1170 1170 a, c, g dbSNP:777359946
1171 1171 a, g dbSNP:192303222
1173 1173 a, c, t dbSNP:778401578
1174 1174 a, g dbSNP:150228971
1176 1176 a, c dbSNP:775475956
1179 1179 c, t dbSNP:376533026
1180 1180 a, g dbSNP:568567422
1190 1190 a, g dbSNP:776727558
1194 1194 g, t dbSNP:761764731
1197 1197 a, g dbSNP:368160421
1206 1206 a, g dbSNP:765543265
1215 1215 a, g dbSNP:773289988
1224 1224 a, g dbSNP:763475264
1226 1226 a, g dbSNP:766782678
1241 1241 c, t dbSNP:752125280
1245 1245 c, t dbSNP:755492501
1246 1246 a, g dbSNP:763542069
1248 1248 c, t dbSNP:753430039
1251 1251 a, g dbSNP:747385959
1287 1287 g, t dbSNP:768999974
1298 1298 c, t dbSNP:777123162
1304 1304 c, t dbSNP:748593655
1308 1308 a, g dbSNP:377758918
1310 1310 a, g dbSNP:773810168
1428 1428 a, t dbSNP:376076399
1443 1443 c, t dbSNP:547923048
1447 1447 a, g dbSNP:180968801
1455 1455 c, t dbSNP:117688975
1461 1461 a, t dbSNP:551772911
1462 1462 -, aa dbSNP:769111521
1504 1504 a, g dbSNP:186424917
1580 1580 a, g dbSNP:137899556
1600 1600 a, g dbSNP:367915009
1624 1624 a, t dbSNP:555061450
1657 1657 c, t dbSNP:573364205
1692 1692 c, t dbSNP:534523199

Target ORF information:

RefSeq Version XM_011529978
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529978

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGATAGCC
TGTTATTCAG CTGGTGACGT CAGTAATTTT CCACAGCAGC CATTCATGAG CTCATGCAGA
CTGTCACAAG GAAATGGGAA TAGCACAATG AGTGTTACAG AAGAAAAAAA GATCCTGTTT
CTTTGCCATC ACATATTATA CACAGACTTA GGGAATCTAG ACTGGAGCAC AAGATATGTG
GGAGGGGTTA GAACACCACT CCTACCTGTA GTAATAATCA TTGAAACCAC TTATTGGATG
CCTTCTGCTT ACCAGACACT AGTTGTCACA GGTCACTGTA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56200
Accession Version XM_011529979.1
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTTTCCTTC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528281.1
CDS60..1502
Misc Feature(1)108..1439(+)
Misc Feature(2)108..1415(+)
Misc Feature(3)150..1415(+)
Misc Feature(4)315..965(+)
Translation
Position Chain Variation Link
16 16 c, g dbSNP:763900902
20 20 c, t dbSNP:753745335
23 23 c, g, t dbSNP:372357778
24 24 a, t dbSNP:544434425
25 25 a, g, t dbSNP:750649096
27 27 g, t dbSNP:780488636
29 29 c, t dbSNP:747293932
36 36 c, t dbSNP:755335917
37 37 c, t dbSNP:201625622
38 38 c, g, t dbSNP:746438478
39 39 a, t dbSNP:183190870
42 42 c, t dbSNP:745531733
45 45 a, g dbSNP:771578438
48 48 c, t dbSNP:775388314
49 49 g, t dbSNP:200713871
51 51 a, g dbSNP:763926844
52 52 g, t dbSNP:560389412
54 54 a, g dbSNP:761671521
55 55 g, t dbSNP:765313611
64 64 c, t dbSNP:143083947
68 68 c, t dbSNP:758676257
70 70 g, t dbSNP:766546584
74 74 a, c, t dbSNP:751781160
75 75 c, g, t dbSNP:140064577
77 77 a, t dbSNP:756636478
78 78 c, g dbSNP:778590882
79 79 a, g dbSNP:377248090
81 81 c, g dbSNP:143916630
82 82 c, g dbSNP:540597292
86 86 g, t dbSNP:146873132
87 87 c, t dbSNP:768340455
89 89 a, c dbSNP:776352156
90 90 g, t dbSNP:761759515
91 91 a, t dbSNP:368501116
92 92 c, g dbSNP:8192454
95 95 a, c dbSNP:773098808
98 98 c, t dbSNP:762989462
99 99 c, t dbSNP:766465184
100 100 a, g, t dbSNP:371824098
101 101 c, t dbSNP:768010749
105 105 c, t dbSNP:753065207
106 106 c, t dbSNP:541051390
113 113 c, t dbSNP:756722245
124 124 c, t dbSNP:778466007
129 129 c, t dbSNP:754349909
132 132 g, t dbSNP:757904153
137 137 a, g dbSNP:779612414
149 149 c, t dbSNP:746654337
150 150 a, c dbSNP:768305498
151 151 a, g dbSNP:5757921
160 160 a, g dbSNP:780994144
172 172 c, t dbSNP:747976292
173 173 a, g dbSNP:377588571
182 182 a, g dbSNP:773188746
183 183 c, t dbSNP:2228415
188 188 c, g dbSNP:770916318
194 194 a, g dbSNP:774435749
199 199 c, g dbSNP:759704594
202 202 a, g dbSNP:767809983
205 205 c, g dbSNP:752938873
206 206 c, t dbSNP:761172321
207 207 c, g dbSNP:764542443
213 213 -, a dbSNP:765158535
220 220 a, g dbSNP:765813625
228 228 a, c, g dbSNP:750871310
232 232 c, t dbSNP:544172142
248 248 a, g dbSNP:767005918
257 257 a, c, g dbSNP:752349207
264 264 c, g dbSNP:200169254
272 272 c, t dbSNP:562234311
275 275 c, g, t dbSNP:145786986
276 276 a, g dbSNP:745787396
283 283 a, g dbSNP:772345709
285 285 a, c dbSNP:145306334
288 288 a, g dbSNP:775646855
290 290 a, c dbSNP:747255804
303 303 a, c dbSNP:768958353
304 304 a, g dbSNP:776685935
306 306 c, t dbSNP:780425464
307 307 a, g dbSNP:765503061
313 313 a, g dbSNP:540648461
314 314 a, g dbSNP:747492297
322 322 c, t dbSNP:149165656
329 329 c, t dbSNP:143275316
330 330 c, t dbSNP:755518176
332 332 c, t dbSNP:148303726
333 333 a, g dbSNP:763852823
341 341 a, g dbSNP:141408072
348 348 c, t dbSNP:757135310
357 357 c, g dbSNP:119450942
364 364 c, g dbSNP:369139561
366 366 g, t dbSNP:761359362
390 390 a, g dbSNP:758355156
395 395 c, t dbSNP:559415958
399 399 c, t dbSNP:374259530
401 401 c, t dbSNP:147017931
406 406 a, g dbSNP:768596079
410 410 c, t dbSNP:781534496
422 422 a, g dbSNP:138203195
436 436 a, g dbSNP:771267221
440 440 a, g dbSNP:774900669
443 443 c, t dbSNP:376894611
457 457 c, g, t dbSNP:768217316
461 461 g, t dbSNP:775948703
465 465 a, g dbSNP:773964967
467 467 c, t dbSNP:368166273
468 468 a, g dbSNP:767397881
473 473 c, g dbSNP:752659791
480 480 c, t dbSNP:756210458
481 481 a, g dbSNP:563054392
486 486 g, t dbSNP:764157818
488 488 c, t dbSNP:754065119
489 489 a, g dbSNP:757413931
497 497 a, t dbSNP:779272702
498 498 a, g dbSNP:746180694
499 499 a, t dbSNP:11089991
504 504 c, g dbSNP:780482118
505 505 a, g dbSNP:747546394
510 510 a, g dbSNP:147194356
524 524 a, g dbSNP:139543370
539 539 c, t dbSNP:149276957
541 541 a, g dbSNP:8192457
544 544 c, g dbSNP:745533324
550 550 a, g dbSNP:775392568
560 560 a, g dbSNP:771772590
570 570 c, t dbSNP:775298300
571 571 a, g dbSNP:760629234
587 587 c, t dbSNP:534209963
600 600 a, g dbSNP:768542145
604 604 a, g dbSNP:776521574
621 621 a, c dbSNP:371892194
622 622 a, g dbSNP:761937276
626 626 c, t dbSNP:765353500
627 627 c, t dbSNP:750614500
628 628 a, g dbSNP:28941471
635 635 a, c dbSNP:754005223
638 638 c, g dbSNP:751928831
640 640 a, g dbSNP:755359802
645 645 c, t dbSNP:376533106
646 646 a, g, t dbSNP:753245184
651 651 a, g dbSNP:778422871
670 670 c, t dbSNP:745524617
675 675 a, g, t dbSNP:148411623
676 676 c, g dbSNP:746848060
679 679 c, g dbSNP:369400468
682 682 a, t dbSNP:776615568
690 690 c, t dbSNP:373777376
698 698 g, t dbSNP:376751756
708 708 c, g dbSNP:199761158
733 733 c, t dbSNP:119450945
760 760 g, t dbSNP:748105288
778 778 c, g dbSNP:754574998
779 779 a, g dbSNP:780996092
793 793 a, g dbSNP:374047157
794 794 a, t dbSNP:143977255
795 795 a, g dbSNP:119450944
810 810 -, gtactgtct dbSNP:769288234
817 817 a, c dbSNP:777822535
819 819 g, t dbSNP:749180603
822 822 c, t dbSNP:771049726
828 828 a, g dbSNP:775115015
829 829 a, g dbSNP:746114979
833 833 g, t dbSNP:78915065
848 848 c, t dbSNP:772388333
859 859 a, c dbSNP:367719924
860 860 c, t dbSNP:369069575
861 861 a, g dbSNP:746501563
864 864 a, g dbSNP:759104126
865 865 -, a dbSNP:774729848
866 866 a, g dbSNP:767008067
867 867 c, t dbSNP:181252862
868 868 a, g dbSNP:760189192
890 890 c, g dbSNP:763973471
893 893 a, g, t dbSNP:2735668
901 901 c, g dbSNP:371529148
906 906 c, g dbSNP:779046571
915 915 c, g dbSNP:376912453
922 922 c, g dbSNP:776328750
929 929 c, t dbSNP:146890981
932 932 a, g dbSNP:765122847
933 933 a, g dbSNP:750393308
938 938 a, g dbSNP:758359453
939 939 c, t dbSNP:8192461
945 945 c, t dbSNP:536254357
946 946 a, g dbSNP:755059672
953 953 c, t dbSNP:145469983
954 954 a, g dbSNP:34396910
957 957 a, c dbSNP:369617680
966 966 c, t dbSNP:373458753
984 984 c, t dbSNP:376357524
985 985 a, g dbSNP:749817666
1012 1012 c, t dbSNP:202064195
1013 1013 a, g dbSNP:774891125
1044 1044 c, t dbSNP:370221257
1045 1045 a, g dbSNP:768203123
1053 1053 c, g dbSNP:776496275
1062 1062 g, t dbSNP:79075278
1068 1068 c, t dbSNP:761493155
1071 1071 c, t dbSNP:769607090
1075 1075 a, t dbSNP:772974251
1076 1076 c, g dbSNP:762710500
1081 1081 c, t dbSNP:770755205
1086 1086 a, g dbSNP:774159147
1088 1088 a, g dbSNP:759459022
1100 1100 c, t dbSNP:767608764
1101 1101 a, g dbSNP:752735865
1112 1112 a, g dbSNP:151095874
1115 1115 a, g dbSNP:764349846
1119 1119 a, g dbSNP:75953451
1120 1120 c, t dbSNP:752238667
1121 1121 a, g dbSNP:757634112
1127 1127 a, g dbSNP:11703124
1131 1131 a, g dbSNP:779273839
1146 1146 a, g dbSNP:375375168
1147 1147 c, t dbSNP:758895348
1148 1148 c, t dbSNP:368373129
1149 1149 a, g dbSNP:370851726
1153 1153 a, g dbSNP:769412149
1164 1164 a, g dbSNP:752104617
1165 1165 c, t dbSNP:755688868
1167 1167 a, g dbSNP:267606256
1170 1170 a, c, g dbSNP:777359946
1171 1171 a, g dbSNP:192303222
1173 1173 a, c, t dbSNP:778401578
1174 1174 a, g dbSNP:150228971
1176 1176 a, c dbSNP:775475956
1179 1179 c, t dbSNP:376533026
1180 1180 a, g dbSNP:568567422
1190 1190 a, g dbSNP:776727558
1194 1194 g, t dbSNP:761764731
1197 1197 a, g dbSNP:368160421
1206 1206 a, g dbSNP:765543265
1215 1215 a, g dbSNP:773289988
1224 1224 a, g dbSNP:763475264
1226 1226 a, g dbSNP:766782678
1241 1241 c, t dbSNP:752125280
1245 1245 c, t dbSNP:755492501
1246 1246 a, g dbSNP:763542069
1248 1248 c, t dbSNP:753430039
1253 1253 c, t dbSNP:779859012
1259 1259 c, t dbSNP:751401941
1266 1266 a, c dbSNP:754714101
1269 1269 -, ag dbSNP:773567586
1271 1271 a, c dbSNP:781253590
1281 1281 c, t dbSNP:747964752
1288 1288 c, t dbSNP:542937686
1294 1294 a, c, t dbSNP:571597579
1303 1303 -, ag dbSNP:760899856
1307 1307 a, g dbSNP:771806949
1311 1311 c, g dbSNP:372650859
1312 1312 a, g dbSNP:774693972
1320 1320 -, aat dbSNP:778107338
1321 1321 a, g dbSNP:145750778
1323 1323 g, t dbSNP:119450943
1329 1329 a, g dbSNP:776070877
1336 1336 a, g dbSNP:119450941
1337 1337 c, t dbSNP:764829606
1338 1338 a, g dbSNP:758241731
1340 1340 c, g dbSNP:762422248
1345 1345 c, t dbSNP:372051078
1347 1347 a, g dbSNP:554254383
1350 1350 a, g dbSNP:200814886
1354 1354 a, c dbSNP:754803189
1364 1364 c, t dbSNP:780788761
1367 1367 g, t dbSNP:61755965
1371 1371 c, t dbSNP:119450940
1381 1381 a, t dbSNP:752702384
1396 1396 -, ctt dbSNP:766428638
1396 1396 a, c dbSNP:755964863
1398 1398 c, t dbSNP:777821034
1399 1399 c, t dbSNP:749427506
1401 1401 c, t dbSNP:771121666
1405 1405 c, t dbSNP:368990043
1408 1408 c, g, t dbSNP:372895468
1409 1409 c, t dbSNP:200300396
1411 1411 a, g dbSNP:377120302
1413 1413 a, c, g dbSNP:572438339
1414 1414 a, g dbSNP:775671027
1427 1427 -, g dbSNP:752585403
1428 1428 a, g dbSNP:747385959
1464 1464 g, t dbSNP:768999974
1475 1475 c, t dbSNP:777123162
1481 1481 c, t dbSNP:748593655
1485 1485 a, g dbSNP:377758918
1487 1487 a, g dbSNP:773810168
1491 1491 a, g dbSNP:757783279
1604 1604 a, g dbSNP:12628249
1605 1605 c, g dbSNP:760632470
1636 1636 a, g dbSNP:530240604
1637 1637 g, t dbSNP:185966243
1669 1669 a, g dbSNP:147186747

Target ORF information:

RefSeq Version XM_011529979
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529979

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGATTGCCAT
GAGAAAATCA GAGTGCTTTC TCAGCAGGCA GCTTCTGTGG TTAAGCAGGA AGGGGGTGAC
AATGACCTCA TAGAGCGTAT CCAGGTTGAT GCCTACTTCA GTCCCATTCA CTCCCAGTTG
GATCATTTAC TGGATCCTTC TTCTTTCACT GGTCGTGCCT CCCAGCAGGT GCAGAGATTC
TTAGAAGAGG AGGTGTATCC CCTGTTAAAA CCATATGAAA GCGTGATGAA GGTTTCCTTC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56201
Accession Version XM_011529980.1
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGTGTAAG
CAAGTGCCAT TGGACAAGAA CGTTGTCTGT GTCATTCCTT GTTGTATTTC CAATAGCAAG
AGCACATCAG ATACTTGGCA ACTAGGCAGT CTTCCAGCTT CTGCCACTGC GCCCGACTTC
CTTGTTGCAT GGATTTATAG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528282.1
CDS60..1463
Misc Feature(1)108..1250(+)
Misc Feature(2)123..965(+)
Misc Feature(3)150..1250(+)
Misc Feature(4)315..965(+)
Translation
Position Chain Variation Link
16 16 c, g dbSNP:763900902
20 20 c, t dbSNP:753745335
23 23 c, g, t dbSNP:372357778
24 24 a, t dbSNP:544434425
25 25 a, g, t dbSNP:750649096
27 27 g, t dbSNP:780488636
29 29 c, t dbSNP:747293932
36 36 c, t dbSNP:755335917
37 37 c, t dbSNP:201625622
38 38 c, g, t dbSNP:746438478
39 39 a, t dbSNP:183190870
42 42 c, t dbSNP:745531733
45 45 a, g dbSNP:771578438
48 48 c, t dbSNP:775388314
49 49 g, t dbSNP:200713871
51 51 a, g dbSNP:763926844
52 52 g, t dbSNP:560389412
54 54 a, g dbSNP:761671521
55 55 g, t dbSNP:765313611
64 64 c, t dbSNP:143083947
68 68 c, t dbSNP:758676257
70 70 g, t dbSNP:766546584
74 74 a, c, t dbSNP:751781160
75 75 c, g, t dbSNP:140064577
77 77 a, t dbSNP:756636478
78 78 c, g dbSNP:778590882
79 79 a, g dbSNP:377248090
81 81 c, g dbSNP:143916630
82 82 c, g dbSNP:540597292
86 86 g, t dbSNP:146873132
87 87 c, t dbSNP:768340455
89 89 a, c dbSNP:776352156
90 90 g, t dbSNP:761759515
91 91 a, t dbSNP:368501116
92 92 c, g dbSNP:8192454
95 95 a, c dbSNP:773098808
98 98 c, t dbSNP:762989462
99 99 c, t dbSNP:766465184
100 100 a, g, t dbSNP:371824098
101 101 c, t dbSNP:768010749
105 105 c, t dbSNP:753065207
106 106 c, t dbSNP:541051390
113 113 c, t dbSNP:756722245
124 124 c, t dbSNP:778466007
129 129 c, t dbSNP:754349909
132 132 g, t dbSNP:757904153
137 137 a, g dbSNP:779612414
149 149 c, t dbSNP:746654337
150 150 a, c dbSNP:768305498
151 151 a, g dbSNP:5757921
160 160 a, g dbSNP:780994144
172 172 c, t dbSNP:747976292
173 173 a, g dbSNP:377588571
182 182 a, g dbSNP:773188746
183 183 c, t dbSNP:2228415
188 188 c, g dbSNP:770916318
194 194 a, g dbSNP:774435749
199 199 c, g dbSNP:759704594
202 202 a, g dbSNP:767809983
205 205 c, g dbSNP:752938873
206 206 c, t dbSNP:761172321
207 207 c, g dbSNP:764542443
213 213 -, a dbSNP:765158535
220 220 a, g dbSNP:765813625
228 228 a, c, g dbSNP:750871310
232 232 c, t dbSNP:544172142
248 248 a, g dbSNP:767005918
257 257 a, c, g dbSNP:752349207
264 264 c, g dbSNP:200169254
272 272 c, t dbSNP:562234311
275 275 c, g, t dbSNP:145786986
276 276 a, g dbSNP:745787396
283 283 a, g dbSNP:772345709
285 285 a, c dbSNP:145306334
288 288 a, g dbSNP:775646855
290 290 a, c dbSNP:747255804
303 303 a, c dbSNP:768958353
304 304 a, g dbSNP:776685935
306 306 c, t dbSNP:780425464
307 307 a, g dbSNP:765503061
313 313 a, g dbSNP:540648461
314 314 a, g dbSNP:747492297
322 322 c, t dbSNP:149165656
329 329 c, t dbSNP:143275316
330 330 c, t dbSNP:755518176
332 332 c, t dbSNP:148303726
333 333 a, g dbSNP:763852823
341 341 a, g dbSNP:141408072
348 348 c, t dbSNP:757135310
357 357 c, g dbSNP:119450942
364 364 c, g dbSNP:369139561
366 366 g, t dbSNP:761359362
390 390 a, g dbSNP:758355156
395 395 c, t dbSNP:559415958
399 399 c, t dbSNP:374259530
401 401 c, t dbSNP:147017931
406 406 a, g dbSNP:768596079
410 410 c, t dbSNP:781534496
422 422 a, g dbSNP:138203195
436 436 a, g dbSNP:771267221
440 440 a, g dbSNP:774900669
443 443 c, t dbSNP:376894611
457 457 c, g, t dbSNP:768217316
461 461 g, t dbSNP:775948703
465 465 a, g dbSNP:773964967
467 467 c, t dbSNP:368166273
468 468 a, g dbSNP:767397881
473 473 c, g dbSNP:752659791
480 480 c, t dbSNP:756210458
481 481 a, g dbSNP:563054392
486 486 g, t dbSNP:764157818
488 488 c, t dbSNP:754065119
489 489 a, g dbSNP:757413931
497 497 a, t dbSNP:779272702
498 498 a, g dbSNP:746180694
499 499 a, t dbSNP:11089991
504 504 c, g dbSNP:780482118
505 505 a, g dbSNP:747546394
510 510 a, g dbSNP:147194356
524 524 a, g dbSNP:139543370
539 539 c, t dbSNP:149276957
541 541 a, g dbSNP:8192457
544 544 c, g dbSNP:745533324
550 550 a, g dbSNP:775392568
560 560 a, g dbSNP:771772590
570 570 c, t dbSNP:775298300
571 571 a, g dbSNP:760629234
587 587 c, t dbSNP:534209963
600 600 a, g dbSNP:768542145
604 604 a, g dbSNP:776521574
621 621 a, c dbSNP:371892194
622 622 a, g dbSNP:761937276
626 626 c, t dbSNP:765353500
627 627 c, t dbSNP:750614500
628 628 a, g dbSNP:28941471
635 635 a, c dbSNP:754005223
638 638 c, g dbSNP:751928831
640 640 a, g dbSNP:755359802
645 645 c, t dbSNP:376533106
646 646 a, g, t dbSNP:753245184
651 651 a, g dbSNP:778422871
670 670 c, t dbSNP:745524617
675 675 a, g, t dbSNP:148411623
676 676 c, g dbSNP:746848060
679 679 c, g dbSNP:369400468
682 682 a, t dbSNP:776615568
690 690 c, t dbSNP:373777376
698 698 g, t dbSNP:376751756
708 708 c, g dbSNP:199761158
733 733 c, t dbSNP:119450945
760 760 g, t dbSNP:748105288
778 778 c, g dbSNP:754574998
779 779 a, g dbSNP:780996092
793 793 a, g dbSNP:374047157
794 794 a, t dbSNP:143977255
795 795 a, g dbSNP:119450944
810 810 -, gtactgtct dbSNP:769288234
817 817 a, c dbSNP:777822535
819 819 g, t dbSNP:749180603
822 822 c, t dbSNP:771049726
828 828 a, g dbSNP:775115015
829 829 a, g dbSNP:746114979
833 833 g, t dbSNP:78915065
848 848 c, t dbSNP:772388333
859 859 a, c dbSNP:367719924
860 860 c, t dbSNP:369069575
861 861 a, g dbSNP:746501563
864 864 a, g dbSNP:759104126
865 865 -, a dbSNP:774729848
866 866 a, g dbSNP:767008067
867 867 c, t dbSNP:181252862
868 868 a, g dbSNP:760189192
890 890 c, g dbSNP:763973471
893 893 a, g, t dbSNP:2735668
901 901 c, g dbSNP:371529148
906 906 c, g dbSNP:779046571
915 915 c, g dbSNP:376912453
922 922 c, g dbSNP:776328750
929 929 c, t dbSNP:146890981
932 932 a, g dbSNP:765122847
933 933 a, g dbSNP:750393308
938 938 a, g dbSNP:758359453
939 939 c, t dbSNP:8192461
945 945 c, t dbSNP:536254357
946 946 a, g dbSNP:755059672
953 953 c, t dbSNP:145469983
954 954 a, g dbSNP:34396910
957 957 a, c dbSNP:369617680
966 966 c, t dbSNP:373458753
984 984 c, t dbSNP:376357524
985 985 a, g dbSNP:749817666
1012 1012 c, t dbSNP:202064195
1013 1013 a, g dbSNP:774891125
1044 1044 c, t dbSNP:370221257
1045 1045 a, g dbSNP:768203123
1053 1053 c, g dbSNP:776496275
1062 1062 g, t dbSNP:79075278
1068 1068 c, t dbSNP:761493155
1071 1071 c, t dbSNP:769607090
1075 1075 a, t dbSNP:772974251
1076 1076 c, g dbSNP:762710500
1081 1081 c, t dbSNP:770755205
1086 1086 a, g dbSNP:774159147
1088 1088 a, g dbSNP:759459022
1100 1100 c, t dbSNP:767608764
1101 1101 a, g dbSNP:752735865
1112 1112 a, g dbSNP:151095874
1115 1115 a, g dbSNP:764349846
1119 1119 a, g dbSNP:75953451
1120 1120 c, t dbSNP:752238667
1121 1121 a, g dbSNP:757634112
1127 1127 a, g dbSNP:11703124
1131 1131 a, g dbSNP:779273839
1146 1146 a, g dbSNP:375375168
1147 1147 c, t dbSNP:758895348
1148 1148 c, t dbSNP:368373129
1149 1149 a, g dbSNP:370851726
1153 1153 a, g dbSNP:769412149
1164 1164 a, g dbSNP:752104617
1165 1165 c, t dbSNP:755688868
1167 1167 a, g dbSNP:267606256
1170 1170 a, c, g dbSNP:777359946
1171 1171 a, g dbSNP:192303222
1173 1173 a, c, t dbSNP:778401578
1174 1174 a, g dbSNP:150228971
1176 1176 a, c dbSNP:775475956
1179 1179 c, t dbSNP:376533026
1180 1180 a, g dbSNP:568567422
1190 1190 a, g dbSNP:776727558
1194 1194 g, t dbSNP:761764731
1197 1197 a, g dbSNP:368160421
1206 1206 a, g dbSNP:765543265
1215 1215 a, g dbSNP:773289988
1224 1224 a, g dbSNP:763475264
1226 1226 a, g dbSNP:766782678
1241 1241 c, t dbSNP:752125280
1245 1245 c, t dbSNP:755492501
1246 1246 a, g dbSNP:763542069
1248 1248 c, t dbSNP:753430039
1251 1251 a, g dbSNP:747385959
1287 1287 g, t dbSNP:768999974
1298 1298 c, t dbSNP:777123162
1304 1304 c, t dbSNP:748593655
1308 1308 a, g dbSNP:377758918
1310 1310 a, g dbSNP:773810168
1323 1323 a, g dbSNP:200511160
1409 1409 c, t dbSNP:536462179
1426 1426 c, g dbSNP:780529556
1429 1429 c, t dbSNP:747343285
1430 1430 a, g dbSNP:374233870
1438 1438 -, c dbSNP:34548480
1464 1464 c, t dbSNP:201506139
1465 1465 a, g, t dbSNP:199650962
1476 1476 g, t dbSNP:534687219
1496 1496 c, t dbSNP:530100881
1522 1522 a, g dbSNP:750305626
1540 1540 a, g dbSNP:146937362
1554 1554 c, t dbSNP:186087225
1555 1555 c, t dbSNP:762256631
1557 1557 g, t dbSNP:769341165
1625 1625 a, g dbSNP:76164361
1627 1627 a, g dbSNP:772564582
1643 1643 a, g dbSNP:577986408
1669 1669 a, g dbSNP:762732535
1686 1686 -, ctaaa dbSNP:553883483
1698 1698 -, a dbSNP:560833492
1719 1719 -, g dbSNP:781250384
1745 1745 a, g dbSNP:753360675
1757 1757 g, t dbSNP:545039108
1762 1762 a, g dbSNP:191857992
1769 1769 c, g dbSNP:111387329
1774 1774 -, g dbSNP:35202382
1806 1806 a, g dbSNP:137939016
1822 1822 c, t dbSNP:542706880
1828 1828 c, t dbSNP:181963971
1829 1829 a, g dbSNP:531516908
1836 1836 c, t dbSNP:528644746
1861 1861 c, t dbSNP:143509180
1892 1892 c, g dbSNP:565412931
1909 1909 c, g dbSNP:532150916
1911 1911 c, t dbSNP:751157422
1930 1930 a, g dbSNP:550269630
1943 1943 a, g dbSNP:759281875
1957 1957 c, t dbSNP:767374254
1962 1962 a, g dbSNP:562577095
1967 1967 g, t dbSNP:529861662
1992 1992 a, c dbSNP:779205808
2008 2008 a, g dbSNP:56082120
2013 2013 c, g, t dbSNP:186535129
2014 2014 c, g, t dbSNP:571388759
2068 2068 a, g dbSNP:533726923
2092 2092 c, t dbSNP:550026663
2096 2096 a, g dbSNP:55739141
2104 2104 c, t dbSNP:571561189
2113 2113 a, g dbSNP:780139835
2117 2117 a, g dbSNP:570612958
2171 2171 a, g dbSNP:539026232
2176 2176 -, ca dbSNP:745389202
2208 2208 c, t dbSNP:557122879
2277 2277 a, g dbSNP:748039605
2328 2328 -, aagag dbSNP:556730420
2347 2347 c, t dbSNP:147177295
2350 2350 a, g dbSNP:769028481
2383 2383 c, t dbSNP:575222704
2402 2402 c, t dbSNP:148139571
2415 2415 g, t dbSNP:748691891
2449 2449 a, g dbSNP:533758774
2451 2451 c, g dbSNP:6001888
2491 2491 a, g dbSNP:115756765
2497 2497 a, g dbSNP:190546028
2498 2498 g, t dbSNP:540439325
2509 2509 c, t dbSNP:566017381
2521 2521 c, t dbSNP:747253177

Target ORF information:

RefSeq Version XM_011529980
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529980

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGGCTG GAGGCGATCA TGGTTCGCCC GACAGCTACC GCTCACCTCT TGCCTCCCGC 
TATGCCAGCC CGGAGATGTG CTTCGTGTTT AGCGACAGGT ATAAATTCCG GACATGGCGG
CAGCTGTGGC TGTGGCTGGC GGAGGCCGAG CAGACATTGG GTTTGCCTAT CACAGATGAA
CAAATCCAGG AGATGAAATC AAACCTGGAG AACATCGACT TCAAGATGGC AGCTGAGGAA
GAGAAACGTT TACGACATGA TGTGATGGCT CACGTGCACA CATTTGGCCA CTGCTGTCCA
AAAGCTGCAG GCATTATTCA CCTTGGTGCT ACTTCTTGCT ATGTTGGAGA CAATACTGAC
TTGATTATTC TTAGAAATGC ACTTGACCTG CTTTTGCCAA AGCTTGCCAG AGTGATCTCT
CGGCTTGCCG ACTTTGCTAA GGAACGAGCC AGTCTACCCA CATTAGGTTT CACACATTTC
CAGCCTGCAC AGCTGACCAC AGTTGGGAAA CGTTGCTGTC TTTGGATTCA GGATCTTTGC
ATGGATCTCC AGAACTTGAA GCGTGTCCGA GATGACCTGC GCTTCCGGGG AGTAAAGGGT
ACCACTGGCA CTCAGGCCAG TTTCCTGCAG CTCTTTGAGG GAGATGACCA TAAGGTAGAG
CAGCTTGACA AGATGGTGAC AGAAAAGGCA GGATTTAAGA GAGCTTTCAT CATCACAGGG
CAGACATATA CACGAAAAGT GGATATTGAA GTACTGTCTG TGCTGGCTAG CTTGGGGGCA
TCAGTGCACA AGATTTGCAC CGACATACGC CTCCTGGCAA ACCTCAAGGA GATGGAGGAA
CCCTTTGAAA AACAGCAGAT TGGCTCAAGT GCGATGCCAT ATAAGCGGAA TCCCATGCGT
TCAGAACGTT GCTGCAGTCT TGCCCGCCAC CTGATGACCC TTGTCATGGA CCCGCTACAG
ACAGCATCTG TCCAGTGGTT TGAACGCACA CTGGATGATA GTGCCAACCG ACGGATCTGT
TTGGCCGAGG CATTTCTTAC CGCAGATACT ATATTGAATA CGCTGCAGAA CATTTCTGAA
GGATTGGTCG TGTACCCCAA AGTAATTGAA CGGCGCATTC GGCAAGAGCT GCCTTTCATG
GCCACAGAGA ACATCATCAT GGCCATGGTC AAAGCTGGAG GTAGCCGCCA GGTGCAGAGA
TTCTTAGAAG AGGAGGTGTA TCCCCTGTTA AAACCATATG AAAGCGTGAT GAAGTGTAAG
CAAGTGCCAT TGGACAAGAA CGTTGTCTGT GTCATTCCTT GTTGTATTTC CAATAGCAAG
AGCACATCAG ATACTTGGCA ACTAGGCAGT CTTCCAGCTT CTGCCACTGC GCCCGACTTC
CTTGTTGCAT GGATTTATAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56202
Accession Version XM_011529981.1
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTTGGAGA CAATACTGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG 
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGATAG CCTGTTATTC AGCTGGTGAC GTCAGTAATT TTCCACAGCA GCCATTCATG
AGCTCATGCA GACTGTCACA AGGAAATGGG AATAGCACAA TGAGTGTTAC AGAAGAAAAA
AAGATCCTGT TTCTTTGCCA TCACATATTA TACACAGACT TAGGGAATCT AGACTGGAGC
ACAAGATATG TGGGAGGGGT TAGAACACCA CTCCTACCTG TAGTAATAAT CATTGAAACC
ACTTATTGGA TGCCTTCTGC TTACCAGACA CTAGTTGTCA CAGGTCACTG TAAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528283.1
CDS382..1638
Misc Feature(1)400..1272(+)
Misc Feature(2)400..1272(+)
Misc Feature(3)637..822(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:753745335
5 5 c, g, t dbSNP:372357778
6 6 a, t dbSNP:544434425
7 7 a, g, t dbSNP:750649096
9 9 g, t dbSNP:780488636
11 11 c, t dbSNP:747293932
18 18 c, t dbSNP:755335917
19 19 c, t dbSNP:201625622
20 20 c, g, t dbSNP:746438478
21 21 a, t dbSNP:183190870
24 24 c, t dbSNP:745531733
27 27 a, g dbSNP:771578438
30 30 c, t dbSNP:775388314
31 31 g, t dbSNP:200713871
33 33 a, g dbSNP:763926844
34 34 g, t dbSNP:560389412
36 36 a, g dbSNP:761671521
37 37 g, t dbSNP:765313611
46 46 c, t dbSNP:143083947
50 50 c, t dbSNP:758676257
52 52 g, t dbSNP:766546584
56 56 a, c, t dbSNP:751781160
57 57 c, g, t dbSNP:140064577
59 59 a, t dbSNP:756636478
60 60 c, g dbSNP:778590882
61 61 a, g dbSNP:377248090
63 63 c, g dbSNP:143916630
64 64 c, g dbSNP:540597292
68 68 g, t dbSNP:146873132
69 69 c, t dbSNP:768340455
71 71 a, c dbSNP:776352156
72 72 g, t dbSNP:761759515
73 73 a, t dbSNP:368501116
74 74 c, g dbSNP:8192454
77 77 a, c dbSNP:773098808
80 80 c, t dbSNP:762989462
81 81 c, t dbSNP:766465184
82 82 a, g, t dbSNP:371824098
83 83 c, t dbSNP:768010749
87 87 c, t dbSNP:753065207
88 88 c, t dbSNP:541051390
95 95 c, t dbSNP:756722245
106 106 c, t dbSNP:778466007
111 111 c, t dbSNP:754349909
114 114 g, t dbSNP:757904153
119 119 a, g dbSNP:779612414
131 131 c, t dbSNP:746654337
132 132 a, c dbSNP:768305498
133 133 a, g dbSNP:5757921
142 142 a, g dbSNP:780994144
154 154 c, t dbSNP:747976292
155 155 a, g dbSNP:377588571
164 164 a, g dbSNP:773188746
165 165 c, t dbSNP:2228415
170 170 c, g dbSNP:770916318
176 176 a, g dbSNP:774435749
181 181 c, g dbSNP:759704594
184 184 a, g dbSNP:767809983
187 187 c, g dbSNP:752938873
188 188 c, t dbSNP:761172321
189 189 c, g dbSNP:764542443
195 195 -, a dbSNP:765158535
202 202 a, g dbSNP:765813625
210 210 a, c, g dbSNP:750871310
214 214 c, t dbSNP:544172142
230 230 a, g dbSNP:767005918
239 239 a, c, g dbSNP:752349207
246 246 c, g dbSNP:200169254
254 254 c, t dbSNP:562234311
257 257 c, g, t dbSNP:145786986
258 258 a, g dbSNP:745787396
265 265 a, g dbSNP:772345709
267 267 a, c dbSNP:145306334
270 270 a, g dbSNP:775646855
272 272 a, c dbSNP:747255804
285 285 a, c dbSNP:768958353
286 286 a, g dbSNP:776685935
288 288 c, t dbSNP:780425464
289 289 a, g dbSNP:765503061
295 295 a, g dbSNP:540648461
296 296 a, g dbSNP:747492297
304 304 c, t dbSNP:149165656
311 311 c, t dbSNP:143275316
312 312 c, t dbSNP:755518176
314 314 c, t dbSNP:148303726
315 315 a, g dbSNP:763852823
323 323 a, g dbSNP:141408072
330 330 c, t dbSNP:757135310
339 339 c, g dbSNP:119450942
346 346 c, g dbSNP:369139561
348 348 g, t dbSNP:761359362
372 372 a, g dbSNP:758355156
377 377 c, t dbSNP:559415958
381 381 c, t dbSNP:374259530
383 383 c, t dbSNP:147017931
388 388 a, g dbSNP:768596079
392 392 c, t dbSNP:781534496
401 401 c, g dbSNP:745533324
407 407 a, g dbSNP:775392568
417 417 a, g dbSNP:771772590
427 427 c, t dbSNP:775298300
428 428 a, g dbSNP:760629234
444 444 c, t dbSNP:534209963
457 457 a, g dbSNP:768542145
461 461 a, g dbSNP:776521574
478 478 a, c dbSNP:371892194
479 479 a, g dbSNP:761937276
483 483 c, t dbSNP:765353500
484 484 c, t dbSNP:750614500
485 485 a, g dbSNP:28941471
492 492 a, c dbSNP:754005223
495 495 c, g dbSNP:751928831
497 497 a, g dbSNP:755359802
502 502 c, t dbSNP:376533106
503 503 a, g, t dbSNP:753245184
508 508 a, g dbSNP:778422871
527 527 c, t dbSNP:745524617
532 532 a, g, t dbSNP:148411623
533 533 c, g dbSNP:746848060
536 536 c, g dbSNP:369400468
539 539 a, t dbSNP:776615568
547 547 c, t dbSNP:373777376
555 555 g, t dbSNP:376751756
565 565 c, g dbSNP:199761158
590 590 c, t dbSNP:119450945
617 617 g, t dbSNP:748105288
635 635 c, g dbSNP:754574998
636 636 a, g dbSNP:780996092
650 650 a, g dbSNP:374047157
651 651 a, t dbSNP:143977255
652 652 a, g dbSNP:119450944
667 667 -, gtactgtct dbSNP:769288234
674 674 a, c dbSNP:777822535
676 676 g, t dbSNP:749180603
679 679 c, t dbSNP:771049726
685 685 a, g dbSNP:775115015
686 686 a, g dbSNP:746114979
690 690 g, t dbSNP:78915065
705 705 c, t dbSNP:772388333
716 716 a, c dbSNP:367719924
717 717 c, t dbSNP:369069575
718 718 a, g dbSNP:746501563
721 721 a, g dbSNP:759104126
722 722 -, a dbSNP:774729848
723 723 a, g dbSNP:767008067
724 724 c, t dbSNP:181252862
725 725 a, g dbSNP:760189192
747 747 c, g dbSNP:763973471
750 750 a, g, t dbSNP:2735668
758 758 c, g dbSNP:371529148
763 763 c, g dbSNP:779046571
772 772 c, g dbSNP:376912453
779 779 c, g dbSNP:776328750
786 786 c, t dbSNP:146890981
789 789 a, g dbSNP:765122847
790 790 a, g dbSNP:750393308
795 795 a, g dbSNP:758359453
796 796 c, t dbSNP:8192461
802 802 c, t dbSNP:536254357
803 803 a, g dbSNP:755059672
810 810 c, t dbSNP:145469983
811 811 a, g dbSNP:34396910
814 814 a, c dbSNP:369617680
823 823 c, t dbSNP:373458753
841 841 c, t dbSNP:376357524
842 842 a, g dbSNP:749817666
869 869 c, t dbSNP:202064195
870 870 a, g dbSNP:774891125
901 901 c, t dbSNP:370221257
902 902 a, g dbSNP:768203123
910 910 c, g dbSNP:776496275
919 919 g, t dbSNP:79075278
925 925 c, t dbSNP:761493155
928 928 c, t dbSNP:769607090
932 932 a, t dbSNP:772974251
933 933 c, g dbSNP:762710500
938 938 c, t dbSNP:770755205
943 943 a, g dbSNP:774159147
945 945 a, g dbSNP:759459022
957 957 c, t dbSNP:767608764
958 958 a, g dbSNP:752735865
969 969 a, g dbSNP:151095874
972 972 a, g dbSNP:764349846
976 976 a, g dbSNP:75953451
977 977 c, t dbSNP:752238667
978 978 a, g dbSNP:757634112
984 984 a, g dbSNP:11703124
988 988 a, g dbSNP:779273839
1003 1003 a, g dbSNP:375375168
1004 1004 c, t dbSNP:758895348
1005 1005 c, t dbSNP:368373129
1006 1006 a, g dbSNP:370851726
1010 1010 a, g dbSNP:769412149
1021 1021 a, g dbSNP:752104617
1022 1022 c, t dbSNP:755688868
1024 1024 a, g dbSNP:267606256
1027 1027 a, c, g dbSNP:777359946
1028 1028 a, g dbSNP:192303222
1030 1030 a, c, t dbSNP:778401578
1031 1031 a, g dbSNP:150228971
1033 1033 a, c dbSNP:775475956
1036 1036 c, t dbSNP:376533026
1037 1037 a, g dbSNP:568567422
1047 1047 a, g dbSNP:776727558
1051 1051 g, t dbSNP:761764731
1054 1054 a, g dbSNP:368160421
1063 1063 a, g dbSNP:765543265
1072 1072 a, g dbSNP:773289988
1081 1081 a, g dbSNP:763475264
1083 1083 a, g dbSNP:766782678
1098 1098 c, t dbSNP:752125280
1102 1102 c, t dbSNP:755492501
1103 1103 a, g dbSNP:763542069
1105 1105 c, t dbSNP:753430039
1110 1110 c, t dbSNP:779859012
1116 1116 c, t dbSNP:751401941
1123 1123 a, c dbSNP:754714101
1126 1126 -, ag dbSNP:773567586
1128 1128 a, c dbSNP:781253590
1138 1138 c, t dbSNP:747964752
1145 1145 c, t dbSNP:542937686
1151 1151 a, c, t dbSNP:571597579
1160 1160 -, ag dbSNP:760899856
1164 1164 a, g dbSNP:771806949
1168 1168 c, g dbSNP:372650859
1169 1169 a, g dbSNP:774693972
1177 1177 -, aat dbSNP:778107338
1178 1178 a, g dbSNP:145750778
1180 1180 g, t dbSNP:119450943
1186 1186 a, g dbSNP:776070877
1193 1193 a, g dbSNP:119450941
1194 1194 c, t dbSNP:764829606
1195 1195 a, g dbSNP:758241731
1197 1197 c, g dbSNP:762422248
1202 1202 c, t dbSNP:372051078
1204 1204 a, g dbSNP:554254383
1207 1207 a, g dbSNP:200814886
1211 1211 a, c dbSNP:754803189
1221 1221 c, t dbSNP:780788761
1224 1224 g, t dbSNP:61755965
1228 1228 c, t dbSNP:119450940
1238 1238 a, t dbSNP:752702384
1253 1253 -, ctt dbSNP:766428638
1253 1253 a, c dbSNP:755964863
1255 1255 c, t dbSNP:777821034
1256 1256 c, t dbSNP:749427506
1258 1258 c, t dbSNP:771121666
1262 1262 c, t dbSNP:368990043
1265 1265 c, g, t dbSNP:372895468
1266 1266 c, t dbSNP:200300396
1268 1268 a, g dbSNP:377120302
1270 1270 a, c, g dbSNP:572438339
1271 1271 a, g dbSNP:775671027
1284 1284 -, g dbSNP:752585403
1285 1285 a, g dbSNP:747385959
1321 1321 g, t dbSNP:768999974
1332 1332 c, t dbSNP:777123162
1338 1338 c, t dbSNP:748593655
1342 1342 a, g dbSNP:377758918
1344 1344 a, g dbSNP:773810168
1462 1462 a, t dbSNP:376076399
1477 1477 c, t dbSNP:547923048
1481 1481 a, g dbSNP:180968801
1489 1489 c, t dbSNP:117688975
1495 1495 a, t dbSNP:551772911
1496 1496 -, aa dbSNP:769111521
1538 1538 a, g dbSNP:186424917
1614 1614 a, g dbSNP:137899556
1634 1634 a, g dbSNP:367915009
1658 1658 a, t dbSNP:555061450
1691 1691 c, t dbSNP:573364205
1726 1726 c, t dbSNP:534523199

Target ORF information:

RefSeq Version XM_011529981
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adenylosuccinate lyase (ADSL), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529981

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTTGGAGA CAATACTGCC TGCACAGCTG ACCACAGTTG GGAAACGTTG CTGTCTTTGG 
ATTCAGGATC TTTGCATGGA TCTCCAGAAC TTGAAGCGTG TCCGAGATGA CCTGCGCTTC
CGGGGAGTAA AGGGTACCAC TGGCACTCAG GCCAGTTTCC TGCAGCTCTT TGAGGGAGAT
GACCATAAGG TAGAGCAGCT TGACAAGATG GTGACAGAAA AGGCAGGATT TAAGAGAGCT
TTCATCATCA CAGGGCAGAC ATATACACGA AAAGTGGATA TTGAAGTACT GTCTGTGCTG
GCTAGCTTGG GGGCATCAGT GCACAAGATT TGCACCGACA TACGCCTCCT GGCAAACCTC
AAGGAGATGG AGGAACCCTT TGAAAAACAG CAGATTGGCT CAAGTGCGAT GCCATATAAG
CGGAATCCCA TGCGTTCAGA ACGTTGCTGC AGTCTTGCCC GCCACCTGAT GACCCTTGTC
ATGGACCCGC TACAGACAGC ATCTGTCCAG TGGTTTGAAC GCACACTGGA TGATAGTGCC
AACCGACGGA TCTGTTTGGC CGAGGCATTT CTTACCGCAG ATACTATATT GAATACGCTG
CAGAACATTT CTGAAGGATT GGTCGTGTAC CCCAAAGTAA TTGAACGGCG CATTCGGCAA
GAGCTGCCTT TCATGGCCAC AGAGAACATC ATCATGGCCA TGGTCAAAGC TGGAGGTAGC
CGCCAGGATT GCCATGAGAA AATCAGAGTG CTTTCTCAGC AGGCAGCTTC TGTGGTTAAG
CAGGAAGGGG GTGACAATGA CCTCATAGAG CGTATCCAGG TTGATGCCTA CTTCAGTCCC
ATTCACTCCC AGTTGGATCA TTTACTGGAT CCTTCTTCTT TCACTGGTCG TGCCTCCCAG
CAGGTGCAGA GATTCTTAGA AGAGGAGGTG TATCCCCTGT TAAAACCATA TGAAAGCGTG
ATGAAGATAG CCTGTTATTC AGCTGGTGAC GTCAGTAATT TTCCACAGCA GCCATTCATG
AGCTCATGCA GACTGTCACA AGGAAATGGG AATAGCACAA TGAGTGTTAC AGAAGAAAAA
AAGATCCTGT TTCTTTGCCA TCACATATTA TACACAGACT TAGGGAATCT AGACTGGAGC
ACAAGATATG TGGGAGGGGT TAGAACACCA CTCCTACCTG TAGTAATAAT CATTGAAACC
ACTTATTGGA TGCCTTCTGC TTACCAGACA CTAGTTGTCA CAGGTCACTG TAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56203
Accession Version XM_011529982.1
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product adenylosuccinate lyase isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGAAC CCTTTGAAAA ACAGCAGATT GGCTCAAGTG CGATGCCATA TAAGCGGAAT 
CCCATGCGTT CAGAACGTTG CTGCAGTCTT GCCCGCCACC TGATGACCCT TGTCATGGAC
CCGCTACAGA CAGCATCTGT CCAGTGGTTT GAACGCACAC TGGATGATAG TGCCAACCGA
CGGATCTGTT TGGCCGAGGC ATTTCTTACC GCAGATACTA TATTGAATAC GCTGCAGAAC
ATTTCTGAAG GATTGGTCGT GTACCCCAAA GTAATTGAAC GGCGCATTCG GCAAGAGCTG
CCTTTCATGG CCACAGAGAA CATCATCATG GCCATGGTCA AAGCTGGAGG TAGCCGCCAG
GATTGCCATG AGAAAATCAG AGTGCTTTCT CAGCAGGCAG CTTCTGTGGT TAAGCAGGAA
GGGGGTGACA ATGACCTCAT AGAGCGTATC CAGGTTGATG CCTACTTCAG TCCCATTCAC
TCCCAGTTGG ATCATTTACT GGATCCTTCT TCTTTCACTG GTCGTGCCTC CCAGCAGGTG
CAGAGATTCT TAGAAGAGGA GGTGTATCCC CTGTTAAAAC CATATGAAAG CGTGATGAAG
ATAGCCTGTT ATTCAGCTGG TGACGTCAGT AATTTTCCAC AGCAGCCATT CATGAGCTCA
TGCAGACTGT CACAAGGAAA TGGGAATAGC ACAATGAGTG TTACAGAAGA AAAAAAGATC
CTGTTTCTTT GCCATCACAT ATTATACACA GACTTAGGGA ATCTAGACTG GAGCACAAGA
TATGTGGGAG GGGTTAGAAC ACCACTCCTA CCTGTAGTAA TAATCATTGA AACCACTTAT
TGGATGCCTT CTGCTTACCA GACACTAGTT GTCACAGGTC ACTGTAAGTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528284.1
CDS431..1321
Misc Feature(1)431..955(+)
Translation
Position Chain Variation Link
1 1 a, g, t dbSNP:753245184
6 6 a, g dbSNP:778422871
25 25 c, t dbSNP:745524617
30 30 a, g, t dbSNP:148411623
31 31 c, g dbSNP:746848060
34 34 c, g dbSNP:369400468
37 37 a, t dbSNP:776615568
45 45 c, t dbSNP:373777376
53 53 g, t dbSNP:376751756
63 63 c, g dbSNP:199761158
88 88 c, t dbSNP:119450945
115 115 g, t dbSNP:748105288
133 133 c, g dbSNP:754574998
134 134 a, g dbSNP:780996092
148 148 a, g dbSNP:374047157
149 149 a, t dbSNP:143977255
150 150 a, g dbSNP:119450944
165 165 -, gtactgtct dbSNP:769288234
172 172 a, c dbSNP:777822535
174 174 g, t dbSNP:749180603
177 177 c, t dbSNP:771049726
183 183 a, g dbSNP:775115015
184 184 a, g dbSNP:746114979
188 188 g, t dbSNP:78915065
203 203 c, t dbSNP:772388333
212 212 c, t dbSNP:542626221
213 213 a, g dbSNP:757597554
229 229 c, t dbSNP:554656225
230 230 a, g dbSNP:572959078
245 245 c, g dbSNP:540433463
268 268 a, g dbSNP:146816578
287 287 a, t dbSNP:2735667
295 295 c, t dbSNP:577710574
304 304 c, t dbSNP:746407220
308 308 c, g dbSNP:758903482
311 311 c, t dbSNP:2743729
335 335 c, g dbSNP:540360032
343 343 a, c dbSNP:8135371
344 344 c, t dbSNP:757212704
349 349 c, t dbSNP:368945643
351 351 c, t dbSNP:371722914
360 360 -, g dbSNP:768933654
369 369 a, g dbSNP:772260021
370 370 a, c dbSNP:780384383
376 376 c, g dbSNP:747194010
381 381 a, g dbSNP:769075937
387 387 a, c dbSNP:377164829
388 388 c, t dbSNP:762409643
399 399 a, c dbSNP:367719924
400 400 c, t dbSNP:369069575
401 401 a, g dbSNP:746501563
404 404 a, g dbSNP:759104126
405 405 -, a dbSNP:774729848
406 406 a, g dbSNP:767008067
407 407 c, t dbSNP:181252862
408 408 a, g dbSNP:760189192
430 430 c, g dbSNP:763973471
433 433 a, g, t dbSNP:2735668
441 441 c, g dbSNP:371529148
446 446 c, g dbSNP:779046571
455 455 c, g dbSNP:376912453
462 462 c, g dbSNP:776328750
469 469 c, t dbSNP:146890981
472 472 a, g dbSNP:765122847
473 473 a, g dbSNP:750393308
478 478 a, g dbSNP:758359453
479 479 c, t dbSNP:8192461
485 485 c, t dbSNP:536254357
486 486 a, g dbSNP:755059672
493 493 c, t dbSNP:145469983
494 494 a, g dbSNP:34396910
497 497 a, c dbSNP:369617680
506 506 c, t dbSNP:373458753
524 524 c, t dbSNP:376357524
525 525 a, g dbSNP:749817666
552 552 c, t dbSNP:202064195
553 553 a, g dbSNP:774891125
584 584 c, t dbSNP:370221257
585 585 a, g dbSNP:768203123
593 593 c, g dbSNP:776496275
602 602 g, t dbSNP:79075278
608 608 c, t dbSNP:761493155
611 611 c, t dbSNP:769607090
615 615 a, t dbSNP:772974251
616 616 c, g dbSNP:762710500
621 621 c, t dbSNP:770755205
626 626 a, g dbSNP:774159147
628 628 a, g dbSNP:759459022
640 640 c, t