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FREM1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol FREM1
Entrez Gene ID 158326
Full Name FRAS1 related extracellular matrix 1
Synonyms BNAR, C9orf143, C9orf145, C9orf154, MOTA, TILRR, TRIGNO2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9p22.3

Summary This gene encodes a basement membrane protein that may play a role in craniofacial and renal development. Mutations in this gene have been associated with bifid nose with or without anorectal and renal anomalies. Alternatively spliced transcript variants encoding different isoforms have been described. PubMed ID 19940113 describes one such variant that initiates transcription within a distinct, internal exon; the resulting shorter isoform (named Toll-like/interleukin-1 receptor regulator, TILRR) is suggested to be a co-receptor of the interleukin 1 receptor family and may regulate receptor function and Toll-like receptor/interleukin 1 receptor signal transduction, contributing to the control of inflammatory response activation. [provided by RefSeq, Apr 2011]. lac of sum
Disorder MIM:

608944

Disorder Html: Bifid nose with or without anorectal and renal anomalies, 608980 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005251382 XP_005251439 FRAS1-related extracellular matrix protein 1 isoform X1
XM_011517748 XP_011516050 FRAS1-related extracellular matrix protein 1 isoform X1
XM_011517749 XP_011516051 FRAS1-related extracellular matrix protein 1 isoform X1
XM_011517750 XP_011516052 FRAS1-related extracellular matrix protein 1 isoform X1
XM_011517751 XP_011516053 FRAS1-related extracellular matrix protein 1 isoform X1
XM_011517752 XP_011516054 FRAS1-related extracellular matrix protein 1 isoform X3
XM_011517753 XP_011516055 FRAS1-related extracellular matrix protein 1 isoform X4
XM_011517754 XP_011516056 FRAS1-related extracellular matrix protein 1 isoform X5
XM_011517755 XP_011516057 FRAS1-related extracellular matrix protein 1 isoform X6
XM_011517756 XP_011516058 FRAS1-related extracellular matrix protein 1 isoform X7
XM_006716726 XP_006716789 FRAS1-related extracellular matrix protein 1 isoform X8
XM_006716728 XP_006716791 FRAS1-related extracellular matrix protein 1 isoform X9
XM_011517757 XP_011516059 FRAS1-related extracellular matrix protein 1 isoform X10
XM_011517758 XP_011516060 FRAS1-related extracellular matrix protein 1 isoform X11
XM_005251384 XP_005251441 FRAS1-related extracellular matrix protein 1 isoform X2
XM_006716729 XP_006716792 FRAS1-related extracellular matrix protein 1 isoform X2
NM_144966 NP_659403 FRAS1-related extracellular matrix protein 1 isoform 1 precursor
NM_001177704 NP_001171175 FRAS1-related extracellular matrix protein 1 isoform 2


Homo sapiens (human) FREM1 NP_659403.4
Pan troglodytes (chimpanzee) FREM1 XP_520492.3
Macaca mulatta (Rhesus monkey) FREM1 XP_001110764.1
Canis lupus familiaris (dog) FREM1 XP_005626693.1
Bos taurus (cattle) FREM1 NP_001179924.1
Mus musculus (house mouse) Frem1 NP_001185740.1
Rattus norvegicus (Norway rat) Frem1 NP_001178686.1
Gallus gallus (chicken) FREM1 XP_424932.2
Danio rerio (zebrafish) frem1a NP_001177237.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100488523 XP_002939136.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22151 XM_005251382 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu22151 XM_011517748 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu22151 XM_011517749 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu22151 XM_011517750 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu22151 XM_011517751 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu76982 XM_011517752 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu76983 XM_011517753 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu76984 XM_011517754 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu76985 XM_011517755 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu76986 XM_011517756 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu42671 XM_006716726 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu42673 XM_006716728 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu76987 XM_011517757 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu76988 XM_011517758 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu22950 XM_005251384 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu22950 XM_006716729 PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu22151 NM_144966 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu22950 NM_001177704 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22151
Clone ID Related Accession (Same CDS sequence) NM_144966 , XM_005251382 , XM_011517748 , XM_011517749 , XM_011517750 , XM_011517751
Accession Version XM_005251382.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced gi:530389733. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005251439.1
CDS787..7326
Misc Feature(1)6022..6273(+)
Misc Feature(2)6973..7317(+)
Misc Feature(3)7231..7299(+)
Translation

Target ORF information:

RefSeq Version XM_005251382
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005251382

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22151
Clone ID Related Accession (Same CDS sequence) NM_144966 , XM_005251382 , XM_011517748 , XM_011517749 , XM_011517750 , XM_011517751
Accession Version XM_011517748.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516050.1
CDS352..6891
Misc Feature(1)5587..5838(+)
Misc Feature(2)6538..6882(+)
Misc Feature(3)6796..6864(+)
Translation

Target ORF information:

RefSeq Version XM_011517748
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517748

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22151
Clone ID Related Accession (Same CDS sequence) NM_144966 , XM_005251382 , XM_011517748 , XM_011517749 , XM_011517750 , XM_011517751
Accession Version XM_011517749.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516051.1
CDS616..7155
Misc Feature(1)5851..6102(+)
Misc Feature(2)6802..7146(+)
Misc Feature(3)7060..7128(+)
Translation

Target ORF information:

RefSeq Version XM_011517749
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517749

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22151
Clone ID Related Accession (Same CDS sequence) NM_144966 , XM_005251382 , XM_011517748 , XM_011517749 , XM_011517750 , XM_011517751
Accession Version XM_011517750.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516052.1
CDS318..6857
Misc Feature(1)5553..5804(+)
Misc Feature(2)6504..6848(+)
Misc Feature(3)6762..6830(+)
Translation

Target ORF information:

RefSeq Version XM_011517750
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517750

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22151
Clone ID Related Accession (Same CDS sequence) NM_144966 , XM_005251382 , XM_011517748 , XM_011517749 , XM_011517750 , XM_011517751
Accession Version XM_011517751.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516053.1
CDS583..7122
Misc Feature(1)5818..6069(+)
Misc Feature(2)6769..7113(+)
Misc Feature(3)7027..7095(+)
Translation

Target ORF information:

RefSeq Version XM_011517751
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517751

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76982
Clone ID Related Accession (Same CDS sequence) XM_011517752
Accession Version XM_011517752.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6390bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAC AATGCCATCC TTCATATTCC TCCAACCAAA GCAAGCACAG CACATGGGAG
AAGGGCATTT GGCATCTGCT GCCCCCAGGG TCTTCCTCAT CCACCACTTC TGGTTCCTTT
CATCTGGAAA GAAGACCTCT TCCATCTTCC ATGCAGCTAG CAGTCATCAG GGGAGACACC
CTGCGGGGCT TTGATTCTAC AGATCTTTCT CAAAGGAAGC TTAGGACCCG TGGGAATGGC
AAAACAGTTC GTCCATCCTC TGTTTATAGA AATGGAACAG ACATCATCTA TAATTATCAT
GGGATAGTTT CCTTGAAACT GGAGGATGAC AGTTTCCCAA CTCACAAAAG GAAGGCCAAA
GTATCCATCA TTAGTCAGCC ACAAAAGACA ATCAAAGTGG CAGAACTGCC TCAAGCAGAT
AAGGTGGAAT CCACAACTGA CTCACACTTC CCCAGACAGG ACCAGTTGCC CTCATTTCCA
AAGAACTGCA CTCTGGAATT AAAGGGACTC TTCCATTTTG AAGAAGGCAT CCAGAAGCTG
TATCAGTGCA ATGGGATCGC CTGGAAAGCC TGGAGTCCCC AAACCAAGGA TGTGGAAGAC
AAATCCTGTC CAGCCGGGTG GCACCAGCAC TCAGGCTACT GTCACATCTT GATCACAGAG
CAGAAAGGCA CCTGGAATGC GGCTGCCCAA GCTTGCAGGG AACAATACCT GGGCAACCTT
GTAACTGTAT TCTCCAGGCA GCACATGCGG TGGCTCTGGG ACATTGGTGG GAGAAAGTCC
TTTTGGATAG GTTTGAACGA CCAAGTGCAT GCTGGCCACT GGGAGTGGAT CGGTGGTGAA
CCTGTTGCCT TCACCAATGG GAGAAGAGGG CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC
TGTGTTTTGG TTCAAAGACA AGGGAAATGG CAAACAAAAG ACTGTAGGAG AGCCAAACCT
CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516054.1
CDS787..7176
Misc Feature(1)6823..7167(+)
Misc Feature(2)7081..7149(+)
Translation

Target ORF information:

RefSeq Version XM_011517752
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517752

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAC AATGCCATCC TTCATATTCC TCCAACCAAA GCAAGCACAG CACATGGGAG
AAGGGCATTT GGCATCTGCT GCCCCCAGGG TCTTCCTCAT CCACCACTTC TGGTTCCTTT
CATCTGGAAA GAAGACCTCT TCCATCTTCC ATGCAGCTAG CAGTCATCAG GGGAGACACC
CTGCGGGGCT TTGATTCTAC AGATCTTTCT CAAAGGAAGC TTAGGACCCG TGGGAATGGC
AAAACAGTTC GTCCATCCTC TGTTTATAGA AATGGAACAG ACATCATCTA TAATTATCAT
GGGATAGTTT CCTTGAAACT GGAGGATGAC AGTTTCCCAA CTCACAAAAG GAAGGCCAAA
GTATCCATCA TTAGTCAGCC ACAAAAGACA ATCAAAGTGG CAGAACTGCC TCAAGCAGAT
AAGGTGGAAT CCACAACTGA CTCACACTTC CCCAGACAGG ACCAGTTGCC CTCATTTCCA
AAGAACTGCA CTCTGGAATT AAAGGGACTC TTCCATTTTG AAGAAGGCAT CCAGAAGCTG
TATCAGTGCA ATGGGATCGC CTGGAAAGCC TGGAGTCCCC AAACCAAGGA TGTGGAAGAC
AAATCCTGTC CAGCCGGGTG GCACCAGCAC TCAGGCTACT GTCACATCTT GATCACAGAG
CAGAAAGGCA CCTGGAATGC GGCTGCCCAA GCTTGCAGGG AACAATACCT GGGCAACCTT
GTAACTGTAT TCTCCAGGCA GCACATGCGG TGGCTCTGGG ACATTGGTGG GAGAAAGTCC
TTTTGGATAG GTTTGAACGA CCAAGTGCAT GCTGGCCACT GGGAGTGGAT CGGTGGTGAA
CCTGTTGCCT TCACCAATGG GAGAAGAGGG CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC
TGTGTTTTGG TTCAAAGACA AGGGAAATGG CAAACAAAAG ACTGTAGGAG AGCCAAACCT
CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76983
Clone ID Related Accession (Same CDS sequence) XM_011517753
Accession Version XM_011517753.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGAT ACCTGGGCAA CCTTGTAACT GTATTCTCCA GGCAGCACAT
GCGGTGGCTC TGGGACATTG GTGGGAGAAA GTCCTTTTGG ATAGGTTTGA ACGACCAAGT
GCATGCTGGC CACTGGGAGT GGATCGGTGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516055.1
CDS787..7059
Misc Feature(1)6022..6273(+)
Translation

Target ORF information:

RefSeq Version XM_011517753
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517753

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGAT ACCTGGGCAA CCTTGTAACT GTATTCTCCA GGCAGCACAT
GCGGTGGCTC TGGGACATTG GTGGGAGAAA GTCCTTTTGG ATAGGTTTGA ACGACCAAGT
GCATGCTGGC CACTGGGAGT GGATCGGTGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76984
Clone ID Related Accession (Same CDS sequence) XM_011517754
Accession Version XM_011517754.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5484bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGTG CAAGTGGAAA TAAAAAAGGC TGGTACTTGC TCTCAAGTGT GTTCCATTGT
GTGAGAAACA CAGACAAGAA TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516056.1
CDS787..6270
Translation

Target ORF information:

RefSeq Version XM_011517754
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517754

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGTG CAAGTGGAAA TAAAAAAGGC TGGTACTTGC TCTCAAGTGT GTTCCATTGT
GTGAGAAACA CAGACAAGAA TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76985
Clone ID Related Accession (Same CDS sequence) XM_011517755
Accession Version XM_011517755.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGCTT CCATGATTGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516057.1
CDS787..6210
Translation

Target ORF information:

RefSeq Version XM_011517755
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517755

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGCTT CCATGATTGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76986
Clone ID Related Accession (Same CDS sequence) XM_011517756
Accession Version XM_011517756.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGTAGATGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516058.1
CDS787..5238
Translation

Target ORF information:

RefSeq Version XM_011517756
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517756

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGTAGATGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu42671
Clone ID Related Accession (Same CDS sequence) XM_006716726
Accession Version XM_006716726.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578816388. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006716789.1
CDS787..5235
Translation

Target ORF information:

RefSeq Version XM_006716726
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716726

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu42673
Clone ID Related Accession (Same CDS sequence) XM_006716728
Accession Version XM_006716728.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578816392. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GGGAGGGACT GGGTTCCTCT CTCCCCTGGC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006716791.1
CDS787..4800
Translation

Target ORF information:

RefSeq Version XM_006716728
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716728

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GGGAGGGACT GGGTTCCTCT CTCCCCTGGC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76987
Clone ID Related Accession (Same CDS sequence) XM_011517757
Accession Version XM_011517757.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GGCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516059.1
CDS787..4773
Translation

Target ORF information:

RefSeq Version XM_011517757
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517757

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GGCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu76988
Clone ID Related Accession (Same CDS sequence) XM_011517758
Accession Version XM_011517758.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2184bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGAGTCCC TGGCACTCAC TCAAGCTTGC ATTTGGATGG TGACACAAGA ATCCATGCTG 
AAGGCTGCCT TGCCTCTCTT TACCAGATTC ATCATCAGCA ATGGACTGCG GACCGAGCAC
GGGGTGTTTG AGATCACACT GGAGACTGTG GACAGAGCCC TGCCTGTGGT AACCAGGAAC
AAGGGGTTGA GACTGGCCCA AGGGGCCGTG GGCCTGCTTT CCCCTGACCT CCTTCAGCTG
ACCGACCCTG ATACACCTGC GGAGAACCTC ACCTTCCTCT TGGTTCAGCT CCCCCAGCAT
GGCCAGCTCT ACCTGTGGGG GACAGGGCTA CTTCAACACA ATTTCACCCA GCAGGATGTG
GACAGCAAGA ATGTGGCCTA TCGGCACTCA GGAGGGGACT CCCAGACTGA CTGCTTTACT
TTCATGGCCA CAGATGGGAC AAACCAAGGC TTTATTGTGA ATGGGAGAGT GTGGGAAGAA
CCTGTTTTAT TCACCATTCA GGTAGACCAA TTGGACAAAA CAGCTCCTCG TATCACACTC
TTGCATTCCC CTTCTCAAGT GGGGCTCCTG AAAAATGGCT GCTACGGGAT TTACATCACT
TCCCGCGTGT TGAAGGCATC AGACCCTGAC ACTGAGGACG ATCAGATCAT CTTTAAAATT
CTACAAGGCC CAAAACATGG ACATCTGGAG AACACAACAA CAGGTGAATT TATCCATGAG
AAATTTAGCC AAAAGGACTT AAACAGTAAG ACTATTCTTT ACATCATAAA CCCATCTTTG
GAAGTAAATT CAGATACCGT GGAATTTCAA ATCATGGACC CCACAGGGAA CTCGGCCACT
CCTCAAATTT TGGAACTGAA GTGGTCTCAT ATTGAATGGT CACAGACCGA ATATGAAGTC
TGTGAGAATG TGGGTTTGTT GCCCTTGGAA ATTATCAGAA GGGGATATTC CATGGACTCG
GCCTTTGTGG GTATAAAGGT CAACCAAGTG TCAGCTGCAG TTGGAAAAGA TTTCACCGTG
ATTCCATCTA AACTGATTCA GTTTGACCCA GGAATGTCAA CTAAGATGTG GAATATAGCA
ATTACCTATG ACGGATTAGA GGAAGATGAT GAGGTCTTTG AAGTAATTCT GAACTCCCCT
GTGAATGCAG TTCTTGGCAC AAAGACAAAA GCTGCAGTGA AAATTTTGGA CTCAAAAGGA
GGACAATGCC ATCCTTCATA TTCCTCCAAC CAAAGCAAGC ACAGCACATG GGAGAAGGGC
ATTTGGCATC TGCTGCCCCC AGGGTCTTCC TCATCCACCA CTTCTGGTTC CTTTCATCTG
GAAAGAAGAC CTCTTCCATC TTCCATGCAG CTAGCAGTCA TCAGGGGAGA CACCCTGCGG
GGCTTTGATT CTACAGATCT TTCTCAAAGG AAGCTTAGGA CCCGTGGGAA TGGCAAAACA
GTTCGTCCAT CCTCTGTTTA TAGAAATGGA ACAGACATCA TCTATAATTA TCATGGGATA
GTTTCCTTGA AACTGGAGGA TGACAGTTTC CCAACTCACA AAAGGAAGGC CAAAGTATCC
ATCATTAGTC AGCCACAAAA GACAATCAAA GTGGCAGAAC TGCCTCAAGC AGATAAGGTG
GAATCCACAA CTGACTCACA CTTCCCCAGA CAGGACCAGT TGCCCTCATT TCCAAAGAAC
TGCACTCTGG AATTAAAGGG ACTCTTCCAT TTTGAAGAAG GCATCCAGAA GCTGTATCAG
TGCAATGGGA TCGCCTGGAA AGCCTGGAGT CCCCAAACCA AGGATGTGGA AGACAAATCC
TGTCCAGCCG GGTGGCACCA GCACTCAGGC TACTGTCACA TCTTGATCAC AGAGCAGAAA
GGCACCTGGA ATGCGGCTGC CCAAGCTTGC AGGGAACAAT ACCTGGGCAA CCTTGTAACT
GTATTCTCCA GGCAGCACAT GCGGTGGCTC TGGGACATTG GTGGGAGAAA GTCCTTTTGG
ATAGGTTTGA ACGACCAAGT GCATGCTGGC CACTGGGAGT GGATCGGTGG TGAACCTGTT
GCCTTCACCA ATGGGAGAAG AGGGCCCTCT CAACGCTCCA AGCTTGGAAA GAGCTGTGTT
TTGGTTCAAA GACAAGGGAA ATGGCAAACA AAAGACTGTA GGAGAGCCAA ACCTCATAAT
TATGTGTGTT CCAGAAAACT CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516060.1
CDS91..2274
Misc Feature(1)970..1221(+)
Misc Feature(2)1921..2265(+)
Misc Feature(3)2179..2247(+)
Translation

Target ORF information:

RefSeq Version XM_011517758
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517758

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGAGTCCC TGGCACTCAC TCAAGCTTGC ATTTGGATGG TGACACAAGA ATCCATGCTG 
AAGGCTGCCT TGCCTCTCTT TACCAGATTC ATCATCAGCA ATGGACTGCG GACCGAGCAC
GGGGTGTTTG AGATCACACT GGAGACTGTG GACAGAGCCC TGCCTGTGGT AACCAGGAAC
AAGGGGTTGA GACTGGCCCA AGGGGCCGTG GGCCTGCTTT CCCCTGACCT CCTTCAGCTG
ACCGACCCTG ATACACCTGC GGAGAACCTC ACCTTCCTCT TGGTTCAGCT CCCCCAGCAT
GGCCAGCTCT ACCTGTGGGG GACAGGGCTA CTTCAACACA ATTTCACCCA GCAGGATGTG
GACAGCAAGA ATGTGGCCTA TCGGCACTCA GGAGGGGACT CCCAGACTGA CTGCTTTACT
TTCATGGCCA CAGATGGGAC AAACCAAGGC TTTATTGTGA ATGGGAGAGT GTGGGAAGAA
CCTGTTTTAT TCACCATTCA GGTAGACCAA TTGGACAAAA CAGCTCCTCG TATCACACTC
TTGCATTCCC CTTCTCAAGT GGGGCTCCTG AAAAATGGCT GCTACGGGAT TTACATCACT
TCCCGCGTGT TGAAGGCATC AGACCCTGAC ACTGAGGACG ATCAGATCAT CTTTAAAATT
CTACAAGGCC CAAAACATGG ACATCTGGAG AACACAACAA CAGGTGAATT TATCCATGAG
AAATTTAGCC AAAAGGACTT AAACAGTAAG ACTATTCTTT ACATCATAAA CCCATCTTTG
GAAGTAAATT CAGATACCGT GGAATTTCAA ATCATGGACC CCACAGGGAA CTCGGCCACT
CCTCAAATTT TGGAACTGAA GTGGTCTCAT ATTGAATGGT CACAGACCGA ATATGAAGTC
TGTGAGAATG TGGGTTTGTT GCCCTTGGAA ATTATCAGAA GGGGATATTC CATGGACTCG
GCCTTTGTGG GTATAAAGGT CAACCAAGTG TCAGCTGCAG TTGGAAAAGA TTTCACCGTG
ATTCCATCTA AACTGATTCA GTTTGACCCA GGAATGTCAA CTAAGATGTG GAATATAGCA
ATTACCTATG ACGGATTAGA GGAAGATGAT GAGGTCTTTG AAGTAATTCT GAACTCCCCT
GTGAATGCAG TTCTTGGCAC AAAGACAAAA GCTGCAGTGA AAATTTTGGA CTCAAAAGGA
GGACAATGCC ATCCTTCATA TTCCTCCAAC CAAAGCAAGC ACAGCACATG GGAGAAGGGC
ATTTGGCATC TGCTGCCCCC AGGGTCTTCC TCATCCACCA CTTCTGGTTC CTTTCATCTG
GAAAGAAGAC CTCTTCCATC TTCCATGCAG CTAGCAGTCA TCAGGGGAGA CACCCTGCGG
GGCTTTGATT CTACAGATCT TTCTCAAAGG AAGCTTAGGA CCCGTGGGAA TGGCAAAACA
GTTCGTCCAT CCTCTGTTTA TAGAAATGGA ACAGACATCA TCTATAATTA TCATGGGATA
GTTTCCTTGA AACTGGAGGA TGACAGTTTC CCAACTCACA AAAGGAAGGC CAAAGTATCC
ATCATTAGTC AGCCACAAAA GACAATCAAA GTGGCAGAAC TGCCTCAAGC AGATAAGGTG
GAATCCACAA CTGACTCACA CTTCCCCAGA CAGGACCAGT TGCCCTCATT TCCAAAGAAC
TGCACTCTGG AATTAAAGGG ACTCTTCCAT TTTGAAGAAG GCATCCAGAA GCTGTATCAG
TGCAATGGGA TCGCCTGGAA AGCCTGGAGT CCCCAAACCA AGGATGTGGA AGACAAATCC
TGTCCAGCCG GGTGGCACCA GCACTCAGGC TACTGTCACA TCTTGATCAC AGAGCAGAAA
GGCACCTGGA ATGCGGCTGC CCAAGCTTGC AGGGAACAAT ACCTGGGCAA CCTTGTAACT
GTATTCTCCA GGCAGCACAT GCGGTGGCTC TGGGACATTG GTGGGAGAAA GTCCTTTTGG
ATAGGTTTGA ACGACCAAGT GCATGCTGGC CACTGGGAGT GGATCGGTGG TGAACCTGTT
GCCTTCACCA ATGGGAGAAG AGGGCCCTCT CAACGCTCCA AGCTTGGAAA GAGCTGTGTT
TTGGTTCAAA GACAAGGGAA ATGGCAAACA AAAGACTGTA GGAGAGCCAA ACCTCATAAT
TATGTGTGTT CCAGAAAACT CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22950
Clone ID Related Accession (Same CDS sequence) NM_001177704 , XM_005251384 , XM_006716729
Accession Version XM_005251384.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578816395. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005251441.1
CDS92..2239
Misc Feature(1)935..1186(+)
Misc Feature(2)1886..2230(+)
Misc Feature(3)2144..2212(+)
Translation

Target ORF information:

RefSeq Version XM_005251384
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005251384

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22950
Clone ID Related Accession (Same CDS sequence) NM_001177704 , XM_005251384 , XM_006716729
Accession Version XM_006716729.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578816396. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006716792.1
CDS90..2237
Misc Feature(1)933..1184(+)
Misc Feature(2)1884..2228(+)
Misc Feature(3)2142..2210(+)
Translation

Target ORF information:

RefSeq Version XM_006716729
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716729

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22151
Clone ID Related Accession (Same CDS sequence) NM_144966 , XM_005251382 , XM_011517748 , XM_011517749 , XM_011517750 , XM_011517751
Accession Version NM_144966.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354672.27, BX537574.1, DN992860.1, AB160987.1, AL512643.9 and AL390732.10. This sequence is a reference standard in the RefSeqGene project. On Apr 27, 2010 this sequence version replaced gi:122056682. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB160987.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_659403.4
CDS817..7356
Misc Feature(1)679..681(+)
Misc Feature(2)1411..1419(+)
Misc Feature(3)1639..1998(+)
Misc Feature(4)1999..2322(+)
Misc Feature(5)2323..2679(+)
Misc Feature(6)2680..3090(+)
Misc Feature(7)3091..3423(+)
Misc Feature(8)3424..3831(+)
Misc Feature(9)3832..4203(+)
Misc Feature(10)4204..4584(+)
Misc Feature(11)4585..4941(+)
Misc Feature(12)4942..5277(+)
Misc Feature(13)5278..5640(+)
Misc Feature(14)5641..6003(+)
Misc Feature(15)6052..6303(+)
Misc Feature(16)6535..6543(+)
Misc Feature(17)7003..7347(+)
Misc Feature(18)7261..7329(+)
Exon (1)1..226
Gene:FREM1
Gene Synonym:
Exon (2)227..549
Gene:FREM1
Gene Synonym:
Exon (3)550..1050
Gene:FREM1
Gene Synonym:
Exon (4)1051..1145
Gene:FREM1
Gene Synonym:
Exon (5)1146..1447
Gene:FREM1
Gene Synonym:
Exon (6)1448..1644
Gene:FREM1
Gene Synonym:
Exon (7)1645..1968
Gene:FREM1
Gene Synonym:
Exon (8)1969..2077
Gene:FREM1
Gene Synonym:
Exon (9)2078..2209
Gene:FREM1
Gene Synonym:
Exon (10)2210..2554
Gene:FREM1
Gene Synonym:
Exon (11)2555..2697
Gene:FREM1
Gene Synonym:
Exon (12)2698..2894
Gene:FREM1
Gene Synonym:
Exon (13)2895..2985
Gene:FREM1
Gene Synonym:
Exon (14)2986..3153
Gene:FREM1
Gene Synonym:
Exon (15)3154..3362
Gene:FREM1
Gene Synonym:
Exon (16)3363..3456
Gene:FREM1
Gene Synonym:
Exon (17)3457..3709
Gene:FREM1
Gene Synonym:
Exon (18)3710..3904
Gene:FREM1
Gene Synonym:
Exon (19)3905..4090
Gene:FREM1
Gene Synonym:
Exon (20)4091..4287
Gene:FREM1
Gene Synonym:
Exon (21)4288..4510
Gene:FREM1
Gene Synonym:
Exon (22)4511..4655
Gene:FREM1
Gene Synonym:
Exon (23)4656..4797
Gene:FREM1
Gene Synonym:
Exon (24)4798..4993
Gene:FREM1
Gene Synonym:
Exon (25)4994..5258
Gene:FREM1
Gene Synonym:
Exon (26)5259..5673
Gene:FREM1
Gene Synonym:
Exon (27)5674..5875
Gene:FREM1
Gene Synonym:
Exon (28)5876..6020
Gene:FREM1
Gene Synonym:
Exon (29)6021..6150
Gene:FREM1
Gene Synonym:
Exon (30)6151..6223
Gene:FREM1
Gene Synonym:
Exon (31)6224..6373
Gene:FREM1
Gene Synonym:
Exon (32)6374..6612
Gene:FREM1
Gene Synonym:
Exon (33)6613..6660
Gene:FREM1
Gene Synonym:
Exon (34)6661..6825
Gene:FREM1
Gene Synonym:
Exon (35)6826..6954
Gene:FREM1
Gene Synonym:
Exon (36)6955..7070
Gene:FREM1
Gene Synonym:
Exon (37)7071..7156
Gene:FREM1
Gene Synonym:
Exon (38)7157..10086
Gene:FREM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_144966
Organism Homo sapiens (human)
Definition Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_144966

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22950
Clone ID Related Accession (Same CDS sequence) NM_001177704 , XM_005251384 , XM_006716729
Accession Version NM_001177704.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product FRAS1-related extracellular matrix protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC031064.1, AL512643.9, AB160987.1 and AL390732.10. Transcript Variant: This variant (2) lacks many of the 5' coding exons found in variant 1 and has a different 5' UTR and 5' CDS. The resulting protein, (isoform 2, also known as Toll-like/interleukin-1 receptor regulator, TILRR) has a different N-terminus and is shorter than isoform 1. This isoform has been shown to associate with the extracellular domain of interleukin-1 receptors and magnify inflammatory responses regulated by nuclear factor kappa B. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001171175.1
CDS90..2237
Misc Feature(1)87..89(+)
Misc Feature(2)933..1184(+)
Misc Feature(3)1884..2228(+)
Misc Feature(4)2142..2210(+)
Exon (1)1..86
Gene:FREM1
Gene Synonym:
Exon (2)87..554
Gene:FREM1
Gene Synonym:
Exon (3)555..756
Gene:FREM1
Gene Synonym:
Exon (4)757..901
Gene:FREM1
Gene Synonym:
Exon (5)902..1031
Gene:FREM1
Gene Synonym:
Exon (6)1032..1104
Gene:FREM1
Gene Synonym:
Exon (7)1105..1254
Gene:FREM1
Gene Synonym:
Exon (8)1255..1493
Gene:FREM1
Gene Synonym:
Exon (9)1494..1541
Gene:FREM1
Gene Synonym:
Exon (10)1542..1706
Gene:FREM1
Gene Synonym:
Exon (11)1707..1835
Gene:FREM1
Gene Synonym:
Exon (12)1836..1951
Gene:FREM1
Gene Synonym:
Exon (13)1952..2037
Gene:FREM1
Gene Synonym:
Exon (14)2038..4967
Gene:FREM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177704
Organism Homo sapiens (human)
Definition Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177704

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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