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CYP24A1 cytochrome P450, family 24, subfamily A, polypeptide 1 [Homo sapiens (human)]

Gene Symbol CYP24A1
Entrez Gene ID 1591
Full Name cytochrome P450, family 24, subfamily A, polypeptide 1
Synonyms CP24, CYP24, HCAI, P450-CC24
General protein information
Preferred Names
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Names
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
24-OHase
cytochrome P450 24A1
cytochrome P450-CC24
vitamin D 24-hydroxylase
exo-mitochondrial protein
vitamin D(3) 24-hydroxylase
1,25-@dihydroxyvitamin D3 24-hydroxylase
cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
NP_000773.2
NP_001122387.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q13

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

126065

Disorder Html:





The following CYP24A1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP24A1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26934 XM_005260304 PREDICTED: Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$439
OHu26934 NM_000782 Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$429
OHu26105 NM_001128915 Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu26934
Accession Version XM_005260304.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu26934D_COA.pdf (pdf)
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835802. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005260361.1
CDS407..1951
Misc Feature(1)581..1942(+)
Translation
Position Chain Variation Link
7 7 a, g dbSNP:73913757
22 22 a, g dbSNP:746437890
54 54 -, g dbSNP:139947227
100 100 g, t dbSNP:562743240
101 101 a, t dbSNP:185796101
111 111 a, g dbSNP:374414065
153 153 c, t dbSNP:572044955
189 189 a, g dbSNP:548763425
220 220 a, c dbSNP:8183750
244 244 c, t dbSNP:181127780
253 253 g, t dbSNP:75209626
258 258 c, g dbSNP:576260966
263 263 a, g dbSNP:757906004
272 272 c, t dbSNP:556468258
275 275 c, t dbSNP:539249372
307 307 c, t dbSNP:144737632
313 313 a, g dbSNP:74424092
331 331 c, t dbSNP:73913755
358 358 g, t dbSNP:766762991
364 364 c, t dbSNP:757992155
367 367 -, c dbSNP:755551917
369 369 a, g, t dbSNP:114797662
370 370 a, c dbSNP:549112129
383 383 c, t dbSNP:369813382
384 384 g, t dbSNP:776599088
393 393 c, t dbSNP:763933381
394 394 g, t dbSNP:760366944
395 395 a, t dbSNP:775539697
400 400 -, c dbSNP:749911681
400 400 -, c dbSNP:762776680
400 400 c, g, t dbSNP:749446753
402 402 c, g dbSNP:773223106
403 403 g, t dbSNP:770011831
406 406 c, t dbSNP:748317423
409 409 a, g dbSNP:781367354
418 418 -, c dbSNP:761781677
422 422 a, t dbSNP:755407412
428 428 a, g dbSNP:747328734
429 429 g, t dbSNP:780666646
431 431 a, c dbSNP:758772744
432 432 g, t dbSNP:749957831
435 435 c, t dbSNP:764800017
443 443 a, g dbSNP:756992582
448 448 c, t dbSNP:753594473
460 460 c, g dbSNP:753717673
468 468 -, c dbSNP:774432244
468 468 c, g dbSNP:760588556
475 475 a, g dbSNP:775140934
479 479 c, g dbSNP:140851407
480 480 c, t dbSNP:759330529
484 484 a, c dbSNP:371315145
498 498 c, t dbSNP:769905811
499 499 a, g dbSNP:151204685
501 501 c, t dbSNP:377542537
507 507 a, c, g, t dbSNP:550482750
520 520 g, t dbSNP:61749689
522 522 a, g dbSNP:746336182
527 527 a, g dbSNP:779530102
529 529 g, t dbSNP:756875653
532 532 a, g dbSNP:373973327
540 540 c, t dbSNP:777546694
546 546 c, t dbSNP:755653308
555 555 a, g dbSNP:564693272
556 556 c, t dbSNP:566481776
568 568 a, c, t dbSNP:527394772
575 575 g, t dbSNP:766293945
578 578 c, t dbSNP:761949738
581 581 c, t dbSNP:776627911
582 582 c, t dbSNP:768937201
583 583 a, g dbSNP:760760499
585 585 a, g dbSNP:775954512
594 594 c, g dbSNP:772361457
599 599 c, t dbSNP:746211356
620 620 c, g dbSNP:779619887
623 623 a, t dbSNP:201820243
626 626 c, t dbSNP:748809100
639 639 a, g, t dbSNP:755808640
640 640 g, t dbSNP:61755338
651 651 a, g dbSNP:780879187
656 656 a, g dbSNP:754828396
662 662 c, g dbSNP:751354816
668 668 c, g dbSNP:767854971
693 693 a, t dbSNP:759771999
702 702 c, t dbSNP:748676671
707 707 c, t dbSNP:766805229
711 711 c, g dbSNP:368218305
712 712 c, t dbSNP:549216457
719 719 a, g dbSNP:147642444
726 726 c, t dbSNP:773653979
729 729 a, c, g, t dbSNP:776150853
730 730 a, c dbSNP:768320804
732 732 c, t dbSNP:746625209
739 739 a, g, t dbSNP:758238180
743 743 c, t dbSNP:745661961
747 747 a, g, t dbSNP:757149824
757 757 a, c, g dbSNP:145275834
763 763 c, t dbSNP:755278180
765 765 a, g, t dbSNP:114476330
768 768 a, c dbSNP:763297732
769 769 c, g dbSNP:750735792
770 770 a, c, g dbSNP:762334108
774 774 a, g dbSNP:371481742
775 775 c, t dbSNP:114334215
776 776 a, g dbSNP:760156921
781 781 c, g dbSNP:775180997
782 782 c, t dbSNP:148084028
784 784 a, g dbSNP:116104487
788 788 a, c dbSNP:778708906
789 789 g, t dbSNP:770793591
791 791 a, c dbSNP:149806586
803 803 c, g dbSNP:200891772
811 811 a, g dbSNP:368019359
814 814 c, t dbSNP:751690053
817 817 c, t dbSNP:780430506
818 818 a, c, t dbSNP:750777104
825 825 a, g dbSNP:765598689
827 827 c, t dbSNP:762052884
828 828 a, g, t dbSNP:764570572
831 831 a, g dbSNP:760244872
834 834 -, aag dbSNP:777676129
834 834 a, g dbSNP:374094543
835 835 a, c dbSNP:370328266
836 836 a, g dbSNP:771581144
837 837 c, g dbSNP:759176351
845 845 c, t dbSNP:774042537
846 846 g, t dbSNP:770795964
849 849 c, t dbSNP:139763321
857 857 g, t dbSNP:387907323
863 863 a, g dbSNP:185120393
867 867 a, g dbSNP:769877816
869 869 c, t dbSNP:748060893
872 872 a, c dbSNP:776404100
875 875 a, c, t dbSNP:35873579
876 876 a, g dbSNP:35051736
878 878 g, t dbSNP:757355477
879 879 c, t dbSNP:139655790
881 881 c, t dbSNP:202070065
882 882 a, c, g dbSNP:387907322
885 885 a, g dbSNP:774490793
894 894 a, g dbSNP:201010579
895 895 a, g dbSNP:375561602
896 896 a, g dbSNP:767884853
900 900 a, c dbSNP:754570461
903 903 g, t dbSNP:750971717
904 904 a, g dbSNP:538419090
916 916 a, g dbSNP:569376598
919 919 -, agtgatgaa dbSNP:779455732
924 924 c, t dbSNP:751584599
937 937 c, g dbSNP:773174932
939 939 a, g dbSNP:146404747
949 949 a, g dbSNP:761681149
953 953 g, t dbSNP:769995389
954 954 c, t dbSNP:768073814
956 956 a, g dbSNP:748562543
958 958 c, t dbSNP:2296241
961 961 c, t dbSNP:769239553
966 966 c, t dbSNP:747513004
967 967 a, g dbSNP:571331367
969 969 a, g dbSNP:372187316
977 977 g, t dbSNP:749949677
978 978 a, t dbSNP:778713554
979 979 g, t dbSNP:756800948
981 981 a, g dbSNP:753689741
982 982 c, g dbSNP:562720862
983 983 a, c dbSNP:377696502
985 985 c, t dbSNP:368717988
991 991 c, t dbSNP:767426961
996 996 a, g dbSNP:763084124
997 997 -, a dbSNP:750060081
1003 1003 c, t dbSNP:114709484
1004 1004 a, g dbSNP:770028088
1010 1010 c, g dbSNP:114579367
1013 1013 c, t dbSNP:776885589
1016 1016 a, c, t dbSNP:376607735
1019 1019 a, g dbSNP:372579163
1021 1021 c, t dbSNP:776207583
1022 1022 a, g dbSNP:115260488
1025 1025 a, c, g dbSNP:575579994
1032 1032 a, g dbSNP:138489641
1039 1039 a, c, g dbSNP:150102121
1050 1050 -, t dbSNP:756892018
1058 1058 a, g, t dbSNP:372063233
1059 1059 a, t dbSNP:777353370
1067 1067 a, g dbSNP:755722524
1073 1073 a, t dbSNP:367995295
1082 1082 c, t dbSNP:780928442
1084 1084 -, t dbSNP:751526033
1089 1089 a, g dbSNP:750368658
1094 1094 a, t dbSNP:751551165
1095 1095 a, g dbSNP:766328243
1096 1096 c, t dbSNP:765292387
1101 1101 a, g dbSNP:552660376
1104 1104 a, c dbSNP:764237039
1105 1105 g, t dbSNP:760930451
1113 1113 c, t dbSNP:776026992
1141 1141 a, g dbSNP:114930663
1144 1144 g, t dbSNP:760041455
1149 1149 c, g, t dbSNP:16999131
1150 1150 a, g dbSNP:6068816
1159 1159 a, g dbSNP:377533549
1165 1165 g, t dbSNP:773564044
1177 1177 c, t dbSNP:202109000
1180 1180 c, g dbSNP:190860407
1181 1181 c, t dbSNP:779721022
1182 1182 c, t dbSNP:373902459
1187 1187 a, g dbSNP:776333239
1189 1189 a, g dbSNP:199934501
1194 1194 c, t dbSNP:746821511
1201 1201 c, t dbSNP:779851763
1205 1205 g, t dbSNP:771933784
1208 1208 c, t dbSNP:745856178
1210 1210 a, g dbSNP:778693884
1213 1213 c, g dbSNP:756319778
1216 1216 a, c, t dbSNP:781564921
1220 1220 a, t dbSNP:755190328
1230 1230 a, g dbSNP:751888651
1235 1235 a, g dbSNP:552082262
1237 1237 c, t dbSNP:368898455
1246 1246 a, c, g dbSNP:528653327
1248 1248 c, g dbSNP:761318027
1255 1255 -, a dbSNP:769114522
1257 1257 a, c dbSNP:200624428
1264 1264 c, t dbSNP:201825042
1265 1265 a, g dbSNP:375976660
1271 1271 a, c dbSNP:774227656
1272 1272 a, g, t dbSNP:749195896
1287 1287 c, t dbSNP:371873677
1291 1291 a, g dbSNP:768841085
1314 1314 c, g dbSNP:76747058
1319 1319 a, g dbSNP:756296470
1327 1327 c, t dbSNP:147022974
1334 1334 a, c dbSNP:758491296
1335 1335 a, g dbSNP:746266995
1336 1336 g, t dbSNP:779209362
1340 1340 g, t dbSNP:757588339
1354 1354 g, t dbSNP:368404770
1357 1357 c, t dbSNP:370297190
1363 1363 a, t dbSNP:184453665
1365 1365 c, t dbSNP:764570392
1370 1370 a, g dbSNP:387907324
1379 1379 a, c dbSNP:752080791
1383 1383 c, t dbSNP:767209123
1387 1387 a, g dbSNP:749064329
1395 1395 c, t dbSNP:552310427
1398 1398 c, g, t dbSNP:557925285
1400 1400 a, g dbSNP:116804918
1402 1402 a, c dbSNP:376545817
1405 1405 -, cagtctaa dbSNP:770055617
1407 1407 a, c, g dbSNP:776602386
1423 1423 c, t dbSNP:772095401
1425 1425 a, g dbSNP:759597034
1426 1426 c, t dbSNP:77764129
1436 1436 c, t dbSNP:771073198
1437 1437 a, g dbSNP:116548533
1445 1445 c, t dbSNP:777947329
1448 1448 a, g dbSNP:770360816
1452 1452 a, t dbSNP:748640858
1479 1479 a, g dbSNP:781707255
1481 1481 c, g dbSNP:779738054
1486 1486 a, g dbSNP:754604586
1487 1487 c, t dbSNP:376508342
1489 1489 c, t dbSNP:147886742
1492 1492 a, g dbSNP:779869928
1494 1494 a, g dbSNP:758075210
1499 1499 g, t dbSNP:750274913
1504 1504 a, t dbSNP:764982769
1505 1505 c, g, t dbSNP:753774862
1506 1506 a, g dbSNP:142282494
1513 1513 -, aga dbSNP:745526991
1527 1527 c, t dbSNP:6022990
1530 1530 c, t dbSNP:189801930
1531 1531 a, g dbSNP:2296239
1538 1538 a, c dbSNP:763117447
1541 1541 a, g dbSNP:773450025
1545 1545 a, g dbSNP:150006710
1549 1549 c, g dbSNP:139729577
1553 1553 c, g dbSNP:777011420
1559 1559 a, g dbSNP:537757911
1560 1560 c, t dbSNP:373730137
1561 1561 g, t dbSNP:779671562
1568 1568 a, g dbSNP:376409540
1569 1569 c, t dbSNP:571886397
1570 1570 a, g dbSNP:373504037
1572 1572 c, t dbSNP:778432343
1573 1573 a, g dbSNP:200816735
1575 1575 a, g dbSNP:749055865
1589 1589 a, g dbSNP:369690314
1592 1592 c, g, t dbSNP:114368325
1593 1593 a, g dbSNP:143934667
1597 1597 a, t dbSNP:781092271
1601 1601 a, g dbSNP:758415328
1603 1603 a, c dbSNP:201318628
1611 1611 c, g dbSNP:750461335
1613 1613 a, g dbSNP:373243941
1624 1624 a, g dbSNP:762056192
1625 1625 a, t dbSNP:140189382
1627 1627 c, t dbSNP:566282976
1629 1629 a, c dbSNP:761110945
1631 1631 c, t dbSNP:776207632
1632 1632 c, t dbSNP:6068812
1634 1634 c, t dbSNP:758978626
1635 1635 c, g dbSNP:752545331
1641 1641 a, g dbSNP:376936695
1645 1645 a, g dbSNP:762355258
1647 1647 c, t dbSNP:535350085
1652 1652 a, g dbSNP:769380230
1661 1661 a, g dbSNP:747960079
1670 1670 c, t dbSNP:776370685
1688 1688 a, g dbSNP:368194785
1694 1694 a, g dbSNP:747021572
1703 1703 a, g dbSNP:566419821
1704 1704 a, g, t dbSNP:555995872
1706 1706 c, g dbSNP:749316674
1713 1713 g, t dbSNP:536086370
1715 1715 a, c dbSNP:756352728
1716 1716 a, c dbSNP:753140812
1721 1721 a, c, t dbSNP:374292194
1722 1722 a, g, t dbSNP:141152573
1723 1723 c, t dbSNP:762452922
1730 1730 c, t dbSNP:772662238
1738 1738 a, g dbSNP:764903433
1739 1739 a, g dbSNP:761493926
1745 1745 -, a dbSNP:781045650
1745 1745 a, g dbSNP:776245104
1752 1752 a, c dbSNP:768556938
1758 1758 c, t dbSNP:200955146
1759 1759 a, g dbSNP:775504814
1761 1761 a, g dbSNP:771844134
1765 1765 a, t dbSNP:749398769
1766 1766 a, c dbSNP:777956236
1772 1772 a, c, g dbSNP:748429181
1774 1774 c, t dbSNP:781257097
1775 1775 a, g, t dbSNP:112596218
1789 1789 a, g dbSNP:751464045
1791 1791 a, g dbSNP:549626881
1793 1793 a, g dbSNP:201594722
1796 1796 g, t dbSNP:750898889
1800 1800 a, g dbSNP:539392099
1803 1803 a, g dbSNP:761303692
1814 1814 a, c dbSNP:766452282
1816 1816 a, t dbSNP:200305803
1821 1821 a, t dbSNP:763589778
1827 1827 -, t dbSNP:756800494
1829 1829 g, t dbSNP:760535613
1835 1835 c, t dbSNP:775095913
1837 1837 c, t dbSNP:143976129
1847 1847 c, t dbSNP:143523685
1848 1848 a, g dbSNP:774497530
1852 1852 a, g dbSNP:770923423
1855 1855 c, t dbSNP:73135773
1856 1856 a, g dbSNP:776534674
1862 1862 c, t dbSNP:768752896
1866 1866 c, t dbSNP:531438091
1871 1871 c, g dbSNP:780242071
1877 1877 a, g dbSNP:563765337
1878 1878 a, g dbSNP:746169287
1880 1880 a, c dbSNP:779409222
1883 1883 a, g dbSNP:757715069
1888 1888 a, g dbSNP:138141685
1889 1889 a, g dbSNP:77167734
1897 1897 c, t dbSNP:777185195
1900 1900 a, g dbSNP:755624262
1903 1903 -, caccctgg dbSNP:758305291
1905 1905 c, g dbSNP:752346395
1906 1906 c, g, t dbSNP:543572030
1907 1907 c, t dbSNP:751340102
1914 1914 c, t dbSNP:766440228
1917 1917 g, t dbSNP:762839799
1919 1919 c, t dbSNP:773181675
1920 1920 a, g dbSNP:146980218
1924 1924 a, c, g dbSNP:775698372
1931 1931 a, g dbSNP:765472830
1932 1932 a, t dbSNP:762094462
1933 1933 c, t dbSNP:61730999
1935 1935 c, t dbSNP:116065115
1936 1936 a, c, g dbSNP:150155645
1946 1946 a, c, t dbSNP:778326571
1950 1950 a, t dbSNP:755645473
1954 1954 c, t dbSNP:747626509
1955 1955 a, g dbSNP:781002878
1969 1969 -, c dbSNP:372131662
1970 1970 a, c dbSNP:573216209
1971 1971 a, g dbSNP:114894168
1988 1988 a, g dbSNP:536478520
1992 1992 aaatggg, gaatggt dbSNP:71337347
1992 1992 a, g dbSNP:36150915
1998 1998 g, t dbSNP:35808408
2019 2019 c, t dbSNP:568550470
2063 2063 a, g dbSNP:34468579
2073 2073 g, t dbSNP:144425017
2080 2080 g, t dbSNP:34419927
2113 2113 a, g dbSNP:71323992
2142 2142 a, g dbSNP:191293339
2173 2173 c, t dbSNP:2762934
2174 2174 a, g dbSNP:552552032
2184 2184 a, g dbSNP:6068811
2200 2200 g, t dbSNP:16999067
2203 2203 a, g dbSNP:560443324
2219 2219 a, g dbSNP:3171182
2231 2231 a, c dbSNP:546776255
2263 2263 c, t dbSNP:4809957
2271 2271 a, g dbSNP:548152637
2324 2324 c, t dbSNP:185779902
2341 2341 a, c dbSNP:397741718
2341 2341 a, c, g dbSNP:55815122
2342 2342 -, a dbSNP:3834669
2346 2346 c, t dbSNP:544298387
2367 2367 c, t dbSNP:546594298
2392 2392 a, c dbSNP:575090104
2440 2440 c, t dbSNP:181138149
2448 2448 a, g dbSNP:545069828
2453 2453 a, g dbSNP:774800506

Target ORF information:

RefSeq Version XM_005260304
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260304

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26934
Accession Version NM_000782.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu26934D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN311231.1, BC109083.1, L13286.1, BI491491.1 and AW022349.1. This sequence is a reference standard in the RefSeqGene project. On Jun 27, 2008 this sequence version replaced gi:55770849. Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: L13286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000773.2
CDS399..1943
Misc Feature(1)330..332(+)
Misc Feature(2)573..1934(+)
Exon (1)1..656
Gene:CYP24A1
Gene Synonym:
Exon (2)657..847
Gene:CYP24A1
Gene Synonym:
Exon (3)848..941
Gene:CYP24A1
Gene Synonym:
Exon (4)942..1038
Gene:CYP24A1
Gene Synonym:
Exon (5)1039..1130
Gene:CYP24A1
Gene Synonym:
Exon (6)1131..1242
Gene:CYP24A1
Gene Synonym:
Exon (7)1243..1388
Gene:CYP24A1
Gene Synonym:
Exon (8)1389..1555
Gene:CYP24A1
Gene Synonym:
Exon (9)1556..1634
Gene:CYP24A1
Gene Synonym:
Exon (10)1635..1832
Gene:CYP24A1
Gene Synonym:
Exon (11)1833..1953
Gene:CYP24A1
Gene Synonym:
Exon (12)1954..3266
Gene:CYP24A1
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:746437890
46 46 -, g dbSNP:139947227
92 92 g, t dbSNP:562743240
93 93 a, t dbSNP:185796101
103 103 a, g dbSNP:374414065
145 145 c, t dbSNP:572044955
181 181 a, g dbSNP:548763425
212 212 a, c dbSNP:8183750
236 236 c, t dbSNP:181127780
245 245 g, t dbSNP:75209626
250 250 c, g dbSNP:576260966
255 255 a, g dbSNP:757906004
264 264 c, t dbSNP:556468258
267 267 c, t dbSNP:539249372
299 299 c, t dbSNP:144737632
305 305 a, g dbSNP:74424092
323 323 c, t dbSNP:73913755
350 350 g, t dbSNP:766762991
356 356 c, t dbSNP:757992155
359 359 -, c dbSNP:755551917
361 361 a, g, t dbSNP:114797662
362 362 a, c dbSNP:549112129
375 375 c, t dbSNP:369813382
376 376 g, t dbSNP:776599088
385 385 c, t dbSNP:763933381
386 386 g, t dbSNP:760366944
387 387 a, t dbSNP:775539697
392 392 -, c dbSNP:749911681
392 392 -, c dbSNP:762776680
392 392 c, g, t dbSNP:749446753
394 394 c, g dbSNP:773223106
395 395 g, t dbSNP:770011831
398 398 c, t dbSNP:748317423
401 401 a, g dbSNP:781367354
410 410 -, c dbSNP:761781677
414 414 a, t dbSNP:755407412
420 420 a, g dbSNP:747328734
421 421 g, t dbSNP:780666646
423 423 a, c dbSNP:758772744
424 424 g, t dbSNP:749957831
427 427 c, t dbSNP:764800017
435 435 a, g dbSNP:756992582
440 440 c, t dbSNP:753594473
452 452 c, g dbSNP:753717673
460 460 -, c dbSNP:774432244
460 460 c, g dbSNP:760588556
467 467 a, g dbSNP:775140934
471 471 c, g dbSNP:140851407
472 472 c, t dbSNP:759330529
476 476 a, c dbSNP:371315145
490 490 c, t dbSNP:769905811
491 491 a, g dbSNP:151204685
493 493 c, t dbSNP:377542537
499 499 a, c, g, t dbSNP:550482750
512 512 g, t dbSNP:61749689
514 514 a, g dbSNP:746336182
519 519 a, g dbSNP:779530102
521 521 g, t dbSNP:756875653
524 524 a, g dbSNP:373973327
532 532 c, t dbSNP:777546694
538 538 c, t dbSNP:755653308
547 547 a, g dbSNP:564693272
548 548 c, t dbSNP:566481776
560 560 a, c, t dbSNP:527394772
567 567 g, t dbSNP:766293945
570 570 c, t dbSNP:761949738
573 573 c, t dbSNP:776627911
574 574 c, t dbSNP:768937201
575 575 a, g dbSNP:760760499
577 577 a, g dbSNP:775954512
586 586 c, g dbSNP:772361457
591 591 c, t dbSNP:746211356
612 612 c, g dbSNP:779619887
615 615 a, t dbSNP:201820243
618 618 c, t dbSNP:748809100
631 631 a, g, t dbSNP:755808640
632 632 g, t dbSNP:61755338
643 643 a, g dbSNP:780879187
648 648 a, g dbSNP:754828396
654 654 c, g dbSNP:751354816
660 660 c, g dbSNP:767854971
685 685 a, t dbSNP:759771999
694 694 c, t dbSNP:748676671
699 699 c, t dbSNP:766805229
703 703 c, g dbSNP:368218305
704 704 c, t dbSNP:549216457
711 711 a, g dbSNP:147642444
718 718 c, t dbSNP:773653979
721 721 a, c, g, t dbSNP:776150853
722 722 a, c dbSNP:768320804
724 724 c, t dbSNP:746625209
731 731 a, g, t dbSNP:758238180
735 735 c, t dbSNP:745661961
739 739 a, g, t dbSNP:757149824
749 749 a, c, g dbSNP:145275834
755 755 c, t dbSNP:755278180
757 757 a, g, t dbSNP:114476330
760 760 a, c dbSNP:763297732
761 761 c, g dbSNP:750735792
762 762 a, c, g dbSNP:762334108
766 766 a, g dbSNP:371481742
767 767 c, t dbSNP:114334215
768 768 a, g dbSNP:760156921
773 773 c, g dbSNP:775180997
774 774 c, t dbSNP:148084028
776 776 a, g dbSNP:116104487
780 780 a, c dbSNP:778708906
781 781 g, t dbSNP:770793591
783 783 a, c dbSNP:149806586
795 795 c, g dbSNP:200891772
803 803 a, g dbSNP:368019359
806 806 c, t dbSNP:751690053
809 809 c, t dbSNP:780430506
810 810 a, c, t dbSNP:750777104
817 817 a, g dbSNP:765598689
819 819 c, t dbSNP:762052884
820 820 a, g, t dbSNP:764570572
823 823 a, g dbSNP:760244872
826 826 -, aag dbSNP:777676129
826 826 a, g dbSNP:374094543
827 827 a, c dbSNP:370328266
828 828 a, g dbSNP:771581144
829 829 c, g dbSNP:759176351
837 837 c, t dbSNP:774042537
838 838 g, t dbSNP:770795964
841 841 c, t dbSNP:139763321
849 849 g, t dbSNP:387907323
855 855 a, g dbSNP:185120393
859 859 a, g dbSNP:769877816
861 861 c, t dbSNP:748060893
864 864 a, c dbSNP:776404100
867 867 a, c, t dbSNP:35873579
868 868 a, g dbSNP:35051736
870 870 g, t dbSNP:757355477
871 871 c, t dbSNP:139655790
873 873 c, t dbSNP:202070065
874 874 a, c, g dbSNP:387907322
877 877 a, g dbSNP:774490793
886 886 a, g dbSNP:201010579
887 887 a, g dbSNP:375561602
888 888 a, g dbSNP:767884853
892 892 a, c dbSNP:754570461
895 895 g, t dbSNP:750971717
896 896 a, g dbSNP:538419090
908 908 a, g dbSNP:569376598
911 911 -, agtgatgaa dbSNP:779455732
916 916 c, t dbSNP:751584599
929 929 c, g dbSNP:773174932
931 931 a, g dbSNP:146404747
941 941 a, g dbSNP:761681149
945 945 g, t dbSNP:769995389
946 946 c, t dbSNP:768073814
948 948 a, g dbSNP:748562543
950 950 c, t dbSNP:2296241
953 953 c, t dbSNP:769239553
958 958 c, t dbSNP:747513004
959 959 a, g dbSNP:571331367
961 961 a, g dbSNP:372187316
969 969 g, t dbSNP:749949677
970 970 a, t dbSNP:778713554
971 971 g, t dbSNP:756800948
973 973 a, g dbSNP:753689741
974 974 c, g dbSNP:562720862
975 975 a, c dbSNP:377696502
977 977 c, t dbSNP:368717988
983 983 c, t dbSNP:767426961
988 988 a, g dbSNP:763084124
989 989 -, a dbSNP:750060081
995 995 c, t dbSNP:114709484
996 996 a, g dbSNP:770028088
1002 1002 c, g dbSNP:114579367
1005 1005 c, t dbSNP:776885589
1008 1008 a, c, t dbSNP:376607735
1011 1011 a, g dbSNP:372579163
1013 1013 c, t dbSNP:776207583
1014 1014 a, g dbSNP:115260488
1017 1017 a, c, g dbSNP:575579994
1024 1024 a, g dbSNP:138489641
1031 1031 a, c, g dbSNP:150102121
1042 1042 -, t dbSNP:756892018
1050 1050 a, g, t dbSNP:372063233
1051 1051 a, t dbSNP:777353370
1059 1059 a, g dbSNP:755722524
1065 1065 a, t dbSNP:367995295
1074 1074 c, t dbSNP:780928442
1076 1076 -, t dbSNP:751526033
1081 1081 a, g dbSNP:750368658
1086 1086 a, t dbSNP:751551165
1087 1087 a, g dbSNP:766328243
1088 1088 c, t dbSNP:765292387
1093 1093 a, g dbSNP:552660376
1096 1096 a, c dbSNP:764237039
1097 1097 g, t dbSNP:760930451
1105 1105 c, t dbSNP:776026992
1133 1133 a, g dbSNP:114930663
1136 1136 g, t dbSNP:760041455
1141 1141 c, g, t dbSNP:16999131
1142 1142 a, g dbSNP:6068816
1151 1151 a, g dbSNP:377533549
1157 1157 g, t dbSNP:773564044
1169 1169 c, t dbSNP:202109000
1172 1172 c, g dbSNP:190860407
1173 1173 c, t dbSNP:779721022
1174 1174 c, t dbSNP:373902459
1179 1179 a, g dbSNP:776333239
1181 1181 a, g dbSNP:199934501
1186 1186 c, t dbSNP:746821511
1193 1193 c, t dbSNP:779851763
1197 1197 g, t dbSNP:771933784
1200 1200 c, t dbSNP:745856178
1202 1202 a, g dbSNP:778693884
1205 1205 c, g dbSNP:756319778
1208 1208 a, c, t dbSNP:781564921
1212 1212 a, t dbSNP:755190328
1222 1222 a, g dbSNP:751888651
1227 1227 a, g dbSNP:552082262
1229 1229 c, t dbSNP:368898455
1238 1238 a, c, g dbSNP:528653327
1240 1240 c, g dbSNP:761318027
1247 1247 -, a dbSNP:769114522
1249 1249 a, c dbSNP:200624428
1256 1256 c, t dbSNP:201825042
1257 1257 a, g dbSNP:375976660
1263 1263 a, c dbSNP:774227656
1264 1264 a, g, t dbSNP:749195896
1279 1279 c, t dbSNP:371873677
1283 1283 a, g dbSNP:768841085
1306 1306 c, g dbSNP:76747058
1311 1311 a, g dbSNP:756296470
1319 1319 c, t dbSNP:147022974
1326 1326 a, c dbSNP:758491296
1327 1327 a, g dbSNP:746266995
1328 1328 g, t dbSNP:779209362
1332 1332 g, t dbSNP:757588339
1346 1346 g, t dbSNP:368404770
1349 1349 c, t dbSNP:370297190
1355 1355 a, t dbSNP:184453665
1357 1357 c, t dbSNP:764570392
1362 1362 a, g dbSNP:387907324
1371 1371 a, c dbSNP:752080791
1375 1375 c, t dbSNP:767209123
1379 1379 a, g dbSNP:749064329
1387 1387 c, t dbSNP:552310427
1390 1390 c, g, t dbSNP:557925285
1392 1392 a, g dbSNP:116804918
1394 1394 a, c dbSNP:376545817
1397 1397 -, cagtctaa dbSNP:770055617
1399 1399 a, c, g dbSNP:776602386
1415 1415 c, t dbSNP:772095401
1417 1417 a, g dbSNP:759597034
1418 1418 c, t dbSNP:77764129
1428 1428 c, t dbSNP:771073198
1429 1429 a, g dbSNP:116548533
1437 1437 c, t dbSNP:777947329
1440 1440 a, g dbSNP:770360816
1444 1444 a, t dbSNP:748640858
1471 1471 a, g dbSNP:781707255
1473 1473 c, g dbSNP:779738054
1478 1478 a, g dbSNP:754604586
1479 1479 c, t dbSNP:376508342
1481 1481 c, t dbSNP:147886742
1484 1484 a, g dbSNP:779869928
1486 1486 a, g dbSNP:758075210
1491 1491 g, t dbSNP:750274913
1496 1496 a, t dbSNP:764982769
1497 1497 c, g, t dbSNP:753774862
1498 1498 a, g dbSNP:142282494
1505 1505 -, aga dbSNP:745526991
1519 1519 c, t dbSNP:6022990
1522 1522 c, t dbSNP:189801930
1523 1523 a, g dbSNP:2296239
1530 1530 a, c dbSNP:763117447
1533 1533 a, g dbSNP:773450025
1537 1537 a, g dbSNP:150006710
1541 1541 c, g dbSNP:139729577
1545 1545 c, g dbSNP:777011420
1551 1551 a, g dbSNP:537757911
1552 1552 c, t dbSNP:373730137
1553 1553 g, t dbSNP:779671562
1560 1560 a, g dbSNP:376409540
1561 1561 c, t dbSNP:571886397
1562 1562 a, g dbSNP:373504037
1564 1564 c, t dbSNP:778432343
1565 1565 a, g dbSNP:200816735
1567 1567 a, g dbSNP:749055865
1581 1581 a, g dbSNP:369690314
1584 1584 c, g, t dbSNP:114368325
1585 1585 a, g dbSNP:143934667
1589 1589 a, t dbSNP:781092271
1593 1593 a, g dbSNP:758415328
1595 1595 a, c dbSNP:201318628
1603 1603 c, g dbSNP:750461335
1605 1605 a, g dbSNP:373243941
1616 1616 a, g dbSNP:762056192
1617 1617 a, t dbSNP:140189382
1619 1619 c, t dbSNP:566282976
1621 1621 a, c dbSNP:761110945
1623 1623 c, t dbSNP:776207632
1624 1624 c, t dbSNP:6068812
1626 1626 c, t dbSNP:758978626
1627 1627 c, g dbSNP:752545331
1633 1633 a, g dbSNP:376936695
1637 1637 a, g dbSNP:762355258
1639 1639 c, t dbSNP:535350085
1644 1644 a, g dbSNP:769380230
1653 1653 a, g dbSNP:747960079
1662 1662 c, t dbSNP:776370685
1680 1680 a, g dbSNP:368194785
1686 1686 a, g dbSNP:747021572
1695 1695 a, g dbSNP:566419821
1696 1696 a, g, t dbSNP:555995872
1698 1698 c, g dbSNP:749316674
1705 1705 g, t dbSNP:536086370
1707 1707 a, c dbSNP:756352728
1708 1708 a, c dbSNP:753140812
1713 1713 a, c, t dbSNP:374292194
1714 1714 a, g, t dbSNP:141152573
1715 1715 c, t dbSNP:762452922
1722 1722 c, t dbSNP:772662238
1730 1730 a, g dbSNP:764903433
1731 1731 a, g dbSNP:761493926
1737 1737 -, a dbSNP:781045650
1737 1737 a, g dbSNP:776245104
1744 1744 a, c dbSNP:768556938
1750 1750 c, t dbSNP:200955146
1751 1751 a, g dbSNP:775504814
1753 1753 a, g dbSNP:771844134
1757 1757 a, t dbSNP:749398769
1758 1758 a, c dbSNP:777956236
1764 1764 a, c, g dbSNP:748429181
1766 1766 c, t dbSNP:781257097
1767 1767 a, g, t dbSNP:112596218
1781 1781 a, g dbSNP:751464045
1783 1783 a, g dbSNP:549626881
1785 1785 a, g dbSNP:201594722
1788 1788 g, t dbSNP:750898889
1792 1792 a, g dbSNP:539392099
1795 1795 a, g dbSNP:761303692
1806 1806 a, c dbSNP:766452282
1808 1808 a, t dbSNP:200305803
1813 1813 a, t dbSNP:763589778
1819 1819 -, t dbSNP:756800494
1821 1821 g, t dbSNP:760535613
1827 1827 c, t dbSNP:775095913
1829 1829 c, t dbSNP:143976129
1839 1839 c, t dbSNP:143523685
1840 1840 a, g dbSNP:774497530
1844 1844 a, g dbSNP:770923423
1847 1847 c, t dbSNP:73135773
1848 1848 a, g dbSNP:776534674
1854 1854 c, t dbSNP:768752896
1858 1858 c, t dbSNP:531438091
1863 1863 c, g dbSNP:780242071
1869 1869 a, g dbSNP:563765337
1870 1870 a, g dbSNP:746169287
1872 1872 a, c dbSNP:779409222
1875 1875 a, g dbSNP:757715069
1880 1880 a, g dbSNP:138141685
1881 1881 a, g dbSNP:77167734
1889 1889 c, t dbSNP:777185195
1892 1892 a, g dbSNP:755624262
1895 1895 -, caccctgg dbSNP:758305291
1897 1897 c, g dbSNP:752346395
1898 1898 c, g, t dbSNP:543572030
1899 1899 c, t dbSNP:751340102
1906 1906 c, t dbSNP:766440228
1909 1909 g, t dbSNP:762839799
1911 1911 c, t dbSNP:773181675
1912 1912 a, g dbSNP:146980218
1916 1916 a, c, g dbSNP:775698372
1923 1923 a, g dbSNP:765472830
1924 1924 a, t dbSNP:762094462
1925 1925 c, t dbSNP:61730999
1927 1927 c, t dbSNP:116065115
1928 1928 a, c, g dbSNP:150155645
1938 1938 a, c, t dbSNP:778326571
1942 1942 a, t dbSNP:755645473
1946 1946 c, t dbSNP:747626509
1947 1947 a, g dbSNP:781002878
1962 1962 a, g dbSNP:191293339
1993 1993 c, t dbSNP:2762934
1994 1994 a, g dbSNP:552552032
2004 2004 a, g dbSNP:6068811
2020 2020 g, t dbSNP:16999067
2023 2023 a, g dbSNP:560443324
2039 2039 a, g dbSNP:3171182
2051 2051 a, c dbSNP:546776255
2083 2083 c, t dbSNP:4809957
2091 2091 a, g dbSNP:548152637
2144 2144 c, t dbSNP:185779902
2161 2161 a, c dbSNP:397741718
2161 2161 a, c, g dbSNP:55815122
2162 2162 -, a dbSNP:3834669
2166 2166 c, t dbSNP:544298387
2187 2187 c, t dbSNP:546594298
2212 2212 a, c dbSNP:575090104
2260 2260 c, t dbSNP:181138149
2268 2268 a, g dbSNP:545069828
2273 2273 a, g dbSNP:774800506
2362 2362 a, g, t dbSNP:188321253
2364 2364 c, t dbSNP:553338669
2381 2381 c, t dbSNP:16999060
2397 2397 a, g dbSNP:761330571
2400 2400 a, c dbSNP:573798919
2459 2459 c, g dbSNP:775991835
2464 2464 g, t dbSNP:554716456
2469 2469 a, g dbSNP:768280947
2477 2477 a, t dbSNP:538344226
2501 2501 c, g dbSNP:569570429
2549 2549 c, t dbSNP:569291645
2583 2583 c, g dbSNP:552524333
2610 2610 a, g dbSNP:185301140
2627 2627 a, g dbSNP:566792866
2641 2641 -, gt dbSNP:147124541
2644 2644 -, tg dbSNP:376783134
2655 2655 -, gt dbSNP:372687331
2658 2658 c, g dbSNP:6022987
2659 2659 a, g dbSNP:75213738
2663 2663 a, g dbSNP:561540935
2691 2691 a, g dbSNP:371011704
2812 2812 -, c dbSNP:34547804
2815 2815 c, t dbSNP:11907350
2828 2828 g, t dbSNP:182056037
2829 2829 -, t dbSNP:36121959
2900 2900 a, t dbSNP:745693216
2929 2929 a, c dbSNP:778847726
2930 2930 c, t dbSNP:118099730
3034 3034 c, t dbSNP:531896457
3050 3050 a, c dbSNP:753877059
3065 3065 a, c dbSNP:564857266
3078 3078 c, t dbSNP:149240951
3125 3125 c, t dbSNP:572891363
3127 3127 a, g dbSNP:756233674
3131 3131 c, t dbSNP:559658233
3137 3137 c, t dbSNP:542943626
3138 3138 a, g dbSNP:752866316
3165 3165 c, t dbSNP:573711673
3198 3198 -, at dbSNP:33948458
3199 3199 -, at dbSNP:10623012
3199 3199 c, g, t dbSNP:111801935
3200 3200 -, at dbSNP:72535210
3200 3200 a, g dbSNP:201783270
3201 3201 -, gt dbSNP:201332611
3209 3209 g, t dbSNP:1063626
3230 3230 a, t dbSNP:543111312
3231 3231 a, g dbSNP:543296744

Target ORF information:

RefSeq Version NM_000782
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000782

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26105
Accession Version NM_001128915.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu26105D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN311231.1, BC109083.1, BI491491.1 and AW022349.1. Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks a coding exon in the 3' region, but the reading frame is not changed, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC109083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAATTG TCCGCAAATA CGACATCCAG
GCCACAGACA ATGAGCCTGT TGAGATGCTA CACTCAGGCA CCCTGGTGCC CAGCCGGGAA
CTCCCCATCG CGTTTTGCCA GCGATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001122387.1
CDS399..1745
Misc Feature(1)330..332(+)
Misc Feature(2)573..>1640(+)
Exon (1)1..656
Gene:CYP24A1
Gene Synonym:
Exon (2)657..847
Gene:CYP24A1
Gene Synonym:
Exon (3)848..941
Gene:CYP24A1
Gene Synonym:
Exon (4)942..1038
Gene:CYP24A1
Gene Synonym:
Exon (5)1039..1130
Gene:CYP24A1
Gene Synonym:
Exon (6)1131..1242
Gene:CYP24A1
Gene Synonym:
Exon (7)1243..1388
Gene:CYP24A1
Gene Synonym:
Exon (8)1389..1555
Gene:CYP24A1
Gene Synonym:
Exon (9)1556..1634
Gene:CYP24A1
Gene Synonym:
Exon (10)1635..1755
Gene:CYP24A1
Gene Synonym:
Exon (11)1756..3068
Gene:CYP24A1
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:746437890
46 46 -, g dbSNP:139947227
92 92 g, t dbSNP:562743240
93 93 a, t dbSNP:185796101
103 103 a, g dbSNP:374414065
145 145 c, t dbSNP:572044955
181 181 a, g dbSNP:548763425
212 212 a, c dbSNP:8183750
236 236 c, t dbSNP:181127780
245 245 g, t dbSNP:75209626
250 250 c, g dbSNP:576260966
255 255 a, g dbSNP:757906004
264 264 c, t dbSNP:556468258
267 267 c, t dbSNP:539249372
299 299 c, t dbSNP:144737632
305 305 a, g dbSNP:74424092
323 323 c, t dbSNP:73913755
350 350 g, t dbSNP:766762991
356 356 c, t dbSNP:757992155
359 359 -, c dbSNP:755551917
361 361 a, g, t dbSNP:114797662
362 362 a, c dbSNP:549112129
375 375 c, t dbSNP:369813382
376 376 g, t dbSNP:776599088
385 385 c, t dbSNP:763933381
386 386 g, t dbSNP:760366944
387 387 a, t dbSNP:775539697
392 392 -, c dbSNP:749911681
392 392 -, c dbSNP:762776680
392 392 c, g, t dbSNP:749446753
394 394 c, g dbSNP:773223106
395 395 g, t dbSNP:770011831
398 398 c, t dbSNP:748317423
401 401 a, g dbSNP:781367354
410 410 -, c dbSNP:761781677
414 414 a, t dbSNP:755407412
420 420 a, g dbSNP:747328734
421 421 g, t dbSNP:780666646
423 423 a, c dbSNP:758772744
424 424 g, t dbSNP:749957831
427 427 c, t dbSNP:764800017
435 435 a, g dbSNP:756992582
440 440 c, t dbSNP:753594473
452 452 c, g dbSNP:753717673
460 460 -, c dbSNP:774432244
460 460 c, g dbSNP:760588556
467 467 a, g dbSNP:775140934
471 471 c, g dbSNP:140851407
472 472 c, t dbSNP:759330529
476 476 a, c dbSNP:371315145
490 490 c, t dbSNP:769905811
491 491 a, g dbSNP:151204685
493 493 c, t dbSNP:377542537
499 499 a, c, g, t dbSNP:550482750
512 512 g, t dbSNP:61749689
514 514 a, g dbSNP:746336182
519 519 a, g dbSNP:779530102
521 521 g, t dbSNP:756875653
524 524 a, g dbSNP:373973327
532 532 c, t dbSNP:777546694
538 538 c, t dbSNP:755653308
547 547 a, g dbSNP:564693272
548 548 c, t dbSNP:566481776
560 560 a, c, t dbSNP:527394772
567 567 g, t dbSNP:766293945
570 570 c, t dbSNP:761949738
573 573 c, t dbSNP:776627911
574 574 c, t dbSNP:768937201
575 575 a, g dbSNP:760760499
577 577 a, g dbSNP:775954512
586 586 c, g dbSNP:772361457
591 591 c, t dbSNP:746211356
612 612 c, g dbSNP:779619887
615 615 a, t dbSNP:201820243
618 618 c, t dbSNP:748809100
631 631 a, g, t dbSNP:755808640
632 632 g, t dbSNP:61755338
643 643 a, g dbSNP:780879187
648 648 a, g dbSNP:754828396
654 654 c, g dbSNP:751354816
660 660 c, g dbSNP:767854971
685 685 a, t dbSNP:759771999
694 694 c, t dbSNP:748676671
699 699 c, t dbSNP:766805229
703 703 c, g dbSNP:368218305
704 704 c, t dbSNP:549216457
711 711 a, g dbSNP:147642444
718 718 c, t dbSNP:773653979
721 721 a, c, g, t dbSNP:776150853
722 722 a, c dbSNP:768320804
724 724 c, t dbSNP:746625209
731 731 a, g, t dbSNP:758238180
735 735 c, t dbSNP:745661961
739 739 a, g, t dbSNP:757149824
749 749 a, c, g dbSNP:145275834
755 755 c, t dbSNP:755278180
757 757 a, g, t dbSNP:114476330
760 760 a, c dbSNP:763297732
761 761 c, g dbSNP:750735792
762 762 a, c, g dbSNP:762334108
766 766 a, g dbSNP:371481742
767 767 c, t dbSNP:114334215
768 768 a, g dbSNP:760156921
773 773 c, g dbSNP:775180997
774 774 c, t dbSNP:148084028
776 776 a, g dbSNP:116104487
780 780 a, c dbSNP:778708906
781 781 g, t dbSNP:770793591
783 783 a, c dbSNP:149806586
795 795 c, g dbSNP:200891772
803 803 a, g dbSNP:368019359
806 806 c, t dbSNP:751690053
809 809 c, t dbSNP:780430506
810 810 a, c, t dbSNP:750777104
817 817 a, g dbSNP:765598689
819 819 c, t dbSNP:762052884
820 820 a, g, t dbSNP:764570572
823 823 a, g dbSNP:760244872
826 826 -, aag dbSNP:777676129
826 826 a, g dbSNP:374094543
827 827 a, c dbSNP:370328266
828 828 a, g dbSNP:771581144
829 829 c, g dbSNP:759176351
837 837 c, t dbSNP:774042537
838 838 g, t dbSNP:770795964
841 841 c, t dbSNP:139763321
849 849 g, t dbSNP:387907323
855 855 a, g dbSNP:185120393
859 859 a, g dbSNP:769877816
861 861 c, t dbSNP:748060893
864 864 a, c dbSNP:776404100
867 867 a, c, t dbSNP:35873579
868 868 a, g dbSNP:35051736
870 870 g, t dbSNP:757355477
871 871 c, t dbSNP:139655790
873 873 c, t dbSNP:202070065
874 874 a, c, g dbSNP:387907322
877 877 a, g dbSNP:774490793
886 886 a, g dbSNP:201010579
887 887 a, g dbSNP:375561602
888 888 a, g dbSNP:767884853
892 892 a, c dbSNP:754570461
895 895 g, t dbSNP:750971717
896 896 a, g dbSNP:538419090
908 908 a, g dbSNP:569376598
911 911 -, agtgatgaa dbSNP:779455732
916 916 c, t dbSNP:751584599
929 929 c, g dbSNP:773174932
931 931 a, g dbSNP:146404747
941 941 a, g dbSNP:761681149
945 945 g, t dbSNP:769995389
946 946 c, t dbSNP:768073814
948 948 a, g dbSNP:748562543
950 950 c, t dbSNP:2296241
953 953 c, t dbSNP:769239553
958 958 c, t dbSNP:747513004
959 959 a, g dbSNP:571331367
961 961 a, g dbSNP:372187316
969 969 g, t dbSNP:749949677
970 970 a, t dbSNP:778713554
971 971 g, t dbSNP:756800948
973 973 a, g dbSNP:753689741
974 974 c, g dbSNP:562720862
975 975 a, c dbSNP:377696502
977 977 c, t dbSNP:368717988
983 983 c, t dbSNP:767426961
988 988 a, g dbSNP:763084124
989 989 -, a dbSNP:750060081
995 995 c, t dbSNP:114709484
996 996 a, g dbSNP:770028088
1002 1002 c, g dbSNP:114579367
1005 1005 c, t dbSNP:776885589
1008 1008 a, c, t dbSNP:376607735
1011 1011 a, g dbSNP:372579163
1013 1013 c, t dbSNP:776207583
1014 1014 a, g dbSNP:115260488
1017 1017 a, c, g dbSNP:575579994
1024 1024 a, g dbSNP:138489641
1031 1031 a, c, g dbSNP:150102121
1042 1042 -, t dbSNP:756892018
1050 1050 a, g, t dbSNP:372063233
1051 1051 a, t dbSNP:777353370
1059 1059 a, g dbSNP:755722524
1065 1065 a, t dbSNP:367995295
1074 1074 c, t dbSNP:780928442
1076 1076 -, t dbSNP:751526033
1081 1081 a, g dbSNP:750368658
1086 1086 a, t dbSNP:751551165
1087 1087 a, g dbSNP:766328243
1088 1088 c, t dbSNP:765292387
1093 1093 a, g dbSNP:552660376
1096 1096 a, c dbSNP:764237039
1097 1097 g, t dbSNP:760930451
1105 1105 c, t dbSNP:776026992
1133 1133 a, g dbSNP:114930663
1136 1136 g, t dbSNP:760041455
1141 1141 c, g, t dbSNP:16999131
1142 1142 a, g dbSNP:6068816
1151 1151 a, g dbSNP:377533549
1157 1157 g, t dbSNP:773564044
1169 1169 c, t dbSNP:202109000
1172 1172 c, g dbSNP:190860407
1173 1173 c, t dbSNP:779721022
1174 1174 c, t dbSNP:373902459
1179 1179 a, g dbSNP:776333239
1181 1181 a, g dbSNP:199934501
1186 1186 c, t dbSNP:746821511
1193 1193 c, t dbSNP:779851763
1197 1197 g, t dbSNP:771933784
1200 1200 c, t dbSNP:745856178
1202 1202 a, g dbSNP:778693884
1205 1205 c, g dbSNP:756319778
1208 1208 a, c, t dbSNP:781564921
1212 1212 a, t dbSNP:755190328
1222 1222 a, g dbSNP:751888651
1227 1227 a, g dbSNP:552082262
1229 1229 c, t dbSNP:368898455
1238 1238 a, c, g dbSNP:528653327
1240 1240 c, g dbSNP:761318027
1247 1247 -, a dbSNP:769114522
1249 1249 a, c dbSNP:200624428
1256 1256 c, t dbSNP:201825042
1257 1257 a, g dbSNP:375976660
1263 1263 a, c dbSNP:774227656
1264 1264 a, g, t dbSNP:749195896
1279 1279 c, t dbSNP:371873677
1283 1283 a, g dbSNP:768841085
1306 1306 c, g dbSNP:76747058
1311 1311 a, g dbSNP:756296470
1319 1319 c, t dbSNP:147022974
1326 1326 a, c dbSNP:758491296
1327 1327 a, g dbSNP:746266995
1328 1328 g, t dbSNP:779209362
1332 1332 g, t dbSNP:757588339
1346 1346 g, t dbSNP:368404770
1349 1349 c, t dbSNP:370297190
1355 1355 a, t dbSNP:184453665
1357 1357 c, t dbSNP:764570392
1362 1362 a, g dbSNP:387907324
1371 1371 a, c dbSNP:752080791
1375 1375 c, t dbSNP:767209123
1379 1379 a, g dbSNP:749064329
1387 1387 c, t dbSNP:552310427
1390 1390 c, g, t dbSNP:557925285
1392 1392 a, g dbSNP:116804918
1394 1394 a, c dbSNP:376545817
1397 1397 -, cagtctaa dbSNP:770055617
1399 1399 a, c, g dbSNP:776602386
1415 1415 c, t dbSNP:772095401
1417 1417 a, g dbSNP:759597034
1418 1418 c, t dbSNP:77764129
1428 1428 c, t dbSNP:771073198
1429 1429 a, g dbSNP:116548533
1437 1437 c, t dbSNP:777947329
1440 1440 a, g dbSNP:770360816
1444 1444 a, t dbSNP:748640858
1471 1471 a, g dbSNP:781707255
1473 1473 c, g dbSNP:779738054
1478 1478 a, g dbSNP:754604586
1479 1479 c, t dbSNP:376508342
1481 1481 c, t dbSNP:147886742
1484 1484 a, g dbSNP:779869928
1486 1486 a, g dbSNP:758075210
1491 1491 g, t dbSNP:750274913
1496 1496 a, t dbSNP:764982769
1497 1497 c, g, t dbSNP:753774862
1498 1498 a, g dbSNP:142282494
1505 1505 -, aga dbSNP:745526991
1519 1519 c, t dbSNP:6022990
1522 1522 c, t dbSNP:189801930
1523 1523 a, g dbSNP:2296239
1530 1530 a, c dbSNP:763117447
1533 1533 a, g dbSNP:773450025
1537 1537 a, g dbSNP:150006710
1541 1541 c, g dbSNP:139729577
1545 1545 c, g dbSNP:777011420
1551 1551 a, g dbSNP:537757911
1552 1552 c, t dbSNP:373730137
1553 1553 g, t dbSNP:779671562
1560 1560 a, g dbSNP:376409540
1561 1561 c, t dbSNP:571886397
1562 1562 a, g dbSNP:373504037
1564 1564 c, t dbSNP:778432343
1565 1565 a, g dbSNP:200816735
1567 1567 a, g dbSNP:749055865
1581 1581 a, g dbSNP:369690314
1584 1584 c, g, t dbSNP:114368325
1585 1585 a, g dbSNP:143934667
1589 1589 a, t dbSNP:781092271
1593 1593 a, g dbSNP:758415328
1595 1595 a, c dbSNP:201318628
1603 1603 c, g dbSNP:750461335
1605 1605 a, g dbSNP:373243941
1616 1616 a, g dbSNP:762056192
1617 1617 a, t dbSNP:140189382
1619 1619 c, t dbSNP:566282976
1621 1621 a, c dbSNP:761110945
1623 1623 c, t dbSNP:776207632
1624 1624 c, t dbSNP:6068812
1626 1626 c, t dbSNP:758978626
1627 1627 c, g dbSNP:752545331
1633 1633 a, g dbSNP:376936695
1641 1641 c, t dbSNP:143523685
1642 1642 a, g dbSNP:774497530
1646 1646 a, g dbSNP:770923423
1649 1649 c, t dbSNP:73135773
1650 1650 a, g dbSNP:776534674
1656 1656 c, t dbSNP:768752896
1660 1660 c, t dbSNP:531438091
1665 1665 c, g dbSNP:780242071
1671 1671 a, g dbSNP:563765337
1672 1672 a, g dbSNP:746169287
1674 1674 a, c dbSNP:779409222
1677 1677 a, g dbSNP:757715069
1682 1682 a, g dbSNP:138141685
1683 1683 a, g dbSNP:77167734
1691 1691 c, t dbSNP:777185195
1694 1694 a, g dbSNP:755624262
1697 1697 -, caccctgg dbSNP:758305291
1699 1699 c, g dbSNP:752346395
1700 1700 c, g, t dbSNP:543572030
1701 1701 c, t dbSNP:751340102
1708 1708 c, t dbSNP:766440228
1711 1711 g, t dbSNP:762839799
1713 1713 c, t dbSNP:773181675
1714 1714 a, g dbSNP:146980218
1718 1718 a, c, g dbSNP:775698372
1725 1725 a, g dbSNP:765472830
1726 1726 a, t dbSNP:762094462
1727 1727 c, t dbSNP:61730999
1729 1729 c, t dbSNP:116065115
1730 1730 a, c, g dbSNP:150155645
1740 1740 a, c, t dbSNP:778326571
1744 1744 a, t dbSNP:755645473
1748 1748 c, t dbSNP:747626509
1749 1749 a, g dbSNP:781002878
1764 1764 a, g dbSNP:191293339
1795 1795 c, t dbSNP:2762934
1796 1796 a, g dbSNP:552552032
1806 1806 a, g dbSNP:6068811
1822 1822 g, t dbSNP:16999067
1825 1825 a, g dbSNP:560443324
1841 1841 a, g dbSNP:3171182
1853 1853 a, c dbSNP:546776255
1885 1885 c, t dbSNP:4809957
1893 1893 a, g dbSNP:548152637
1946 1946 c, t dbSNP:185779902
1963 1963 a, c dbSNP:397741718
1963 1963 a, c, g dbSNP:55815122
1964 1964 -, a dbSNP:3834669
1968 1968 c, t dbSNP:544298387
1989 1989 c, t dbSNP:546594298
2014 2014 a, c dbSNP:575090104
2062 2062 c, t dbSNP:181138149
2070 2070 a, g dbSNP:545069828
2075 2075 a, g dbSNP:774800506
2164 2164 a, g, t dbSNP:188321253
2166 2166 c, t dbSNP:553338669
2183 2183 c, t dbSNP:16999060
2199 2199 a, g dbSNP:761330571
2202 2202 a, c dbSNP:573798919
2261 2261 c, g dbSNP:775991835
2266 2266 g, t dbSNP:554716456
2271 2271 a, g dbSNP:768280947
2279 2279 a, t dbSNP:538344226
2303 2303 c, g dbSNP:569570429
2351 2351 c, t dbSNP:569291645
2385 2385 c, g dbSNP:552524333
2412 2412 a, g dbSNP:185301140
2429 2429 a, g dbSNP:566792866
2443 2443 -, gt dbSNP:147124541
2446 2446 -, tg dbSNP:376783134
2457 2457 -, gt dbSNP:372687331
2460 2460 c, g dbSNP:6022987
2461 2461 a, g dbSNP:75213738
2465 2465 a, g dbSNP:561540935
2493 2493 a, g dbSNP:371011704
2614 2614 -, c dbSNP:34547804
2617 2617 c, t dbSNP:11907350
2630 2630 g, t dbSNP:182056037
2631 2631 -, t dbSNP:36121959
2702 2702 a, t dbSNP:745693216
2731 2731 a, c dbSNP:778847726
2732 2732 c, t dbSNP:118099730
2836 2836 c, t dbSNP:531896457
2852 2852 a, c dbSNP:753877059
2867 2867 a, c dbSNP:564857266
2880 2880 c, t dbSNP:149240951
2927 2927 c, t dbSNP:572891363
2929 2929 a, g dbSNP:756233674
2933 2933 c, t dbSNP:559658233
2939 2939 c, t dbSNP:542943626
2940 2940 a, g dbSNP:752866316
2967 2967 c, t dbSNP:573711673
3000 3000 -, at dbSNP:33948458
3001 3001 -, at dbSNP:10623012
3001 3001 c, g, t dbSNP:111801935
3002 3002 -, at dbSNP:72535210
3002 3002 a, g dbSNP:201783270
3003 3003 -, gt dbSNP:201332611
3011 3011 g, t dbSNP:1063626
3032 3032 a, t dbSNP:543111312
3033 3033 a, g dbSNP:543296744

Target ORF information:

RefSeq Version NM_001128915
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001128915

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAATTG TCCGCAAATA CGACATCCAG
GCCACAGACA ATGAGCCTGT TGAGATGCTA CACTCAGGCA CCCTGGTGCC CAGCCGGGAA
CTCCCCATCG CGTTTTGCCA GCGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.