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CYP24A1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYP24A1
Entrez Gene ID 1591
Full Name cytochrome P450, family 24, subfamily A, polypeptide 1
Synonyms CP24, CYP24, HCAI, P450-CC24
General protein information
Preferred Names
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Names
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
24-OHase
cytochrome P450 24A1
cytochrome P450-CC24
vitamin D 24-hydroxylase
exo-mitochondrial protein
vitamin D(3) 24-hydroxylase
1,25-@dihydroxyvitamin D3 24-hydroxylase
cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
NP_000773.2
NP_001122387.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q13

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

126065


mRNA and Protein(s)

mRNA Protein Name
XM_005260304 XP_005260361 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform X1
NM_000782 NP_000773 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1 precursor
NM_001128915 NP_001122387 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 2 precursor



Homo sapiens (human) CYP24A1 NP_000773.2
Pan troglodytes (chimpanzee) LOC746729 XP_001154704.2
Macaca mulatta (Rhesus monkey) LOC698937 XP_002798136.1
Canis lupus familiaris (dog) CYP24A1 XP_543059.2
Bos taurus (cattle) CYP24A1 NP_001178346.1
Mus musculus (house mouse) Cyp24a1 NP_034126.1
Rattus norvegicus (Norway rat) Cyp24a1 NP_963966.1
Gallus gallus (chicken) CYP24A1 NP_990310.1
Danio rerio (zebrafish) cyp24a1 NP_001082927.1
Drosophila melanogaster (fruit fly) Cyp12b2 NP_611370.2
Caenorhabditis elegans cyp-44A1 NP_495052.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496967 XP_002941296.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CYP24A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP24A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26934
XM_005260304 PREDICTED: Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$214.50-$300.30
$429.00
OHu26934
NM_000782 Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$214.50-$300.30
$429.00
OHu26105
NM_001128915 Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26934
Clone ID Related Accession (Same CDS sequence) NM_000782 , XM_005260304
Accession Version XM_005260304.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835802. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260361.1
CDS407..1951
Misc Feature(1)581..1942(+)
Translation

Target ORF information:

RefSeq Version XM_005260304
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260304

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26934
Clone ID Related Accession (Same CDS sequence) NM_000782 , XM_005260304
Accession Version NM_000782.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN311231.1, BC109083.1, L13286.1, BI491491.1 and AW022349.1. This sequence is a reference standard in the RefSeqGene project. On Jun 27, 2008 this sequence version replaced gi:55770849. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: L13286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000773.2
CDS399..1943
Misc Feature(1)330..332(+)
Misc Feature(2)573..1934(+)
Exon (1)1..656
Gene:CYP24A1
Gene Synonym:
Exon (2)657..847
Gene:CYP24A1
Gene Synonym:
Exon (3)848..941
Gene:CYP24A1
Gene Synonym:
Exon (4)942..1038
Gene:CYP24A1
Gene Synonym:
Exon (5)1039..1130
Gene:CYP24A1
Gene Synonym:
Exon (6)1131..1242
Gene:CYP24A1
Gene Synonym:
Exon (7)1243..1388
Gene:CYP24A1
Gene Synonym:
Exon (8)1389..1555
Gene:CYP24A1
Gene Synonym:
Exon (9)1556..1634
Gene:CYP24A1
Gene Synonym:
Exon (10)1635..1832
Gene:CYP24A1
Gene Synonym:
Exon (11)1833..1953
Gene:CYP24A1
Gene Synonym:
Exon (12)1954..3266
Gene:CYP24A1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000782
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000782

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAACAG TGCTCATGCT AAATACCCAG
GTGTTGGGAT CCAGTGAAGA CAATTTTGAA GATTCAAGTC AGTTTAGACC TGAACGTTGG
CTTCAGGAGA AGGAAAAAAT TAATCCTTTT GCGCATCTTC CATTTGGCGT TGGAAAAAGA
ATGTGCATTG GTCGCCGATT AGCAGAGCTT CAACTGCATT TGGCTCTTTG TTGGATTGTC
CGCAAATACG ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC
CTGGTGCCCA GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26105
Clone ID Related Accession (Same CDS sequence) NM_001128915
Accession Version NM_001128915.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN311231.1, BC109083.1, BI491491.1 and AW022349.1. Transcript Variant: This variant (2) lacks a coding exon in the 3' region, but the reading frame is not changed, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC109083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAATTG TCCGCAAATA CGACATCCAG
GCCACAGACA ATGAGCCTGT TGAGATGCTA CACTCAGGCA CCCTGGTGCC CAGCCGGGAA
CTCCCCATCG CGTTTTGCCA GCGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001122387.1
CDS399..1745
Misc Feature(1)330..332(+)
Misc Feature(2)573..>1640(+)
Exon (1)1..656
Gene:CYP24A1
Gene Synonym:
Exon (2)657..847
Gene:CYP24A1
Gene Synonym:
Exon (3)848..941
Gene:CYP24A1
Gene Synonym:
Exon (4)942..1038
Gene:CYP24A1
Gene Synonym:
Exon (5)1039..1130
Gene:CYP24A1
Gene Synonym:
Exon (6)1131..1242
Gene:CYP24A1
Gene Synonym:
Exon (7)1243..1388
Gene:CYP24A1
Gene Synonym:
Exon (8)1389..1555
Gene:CYP24A1
Gene Synonym:
Exon (9)1556..1634
Gene:CYP24A1
Gene Synonym:
Exon (10)1635..1755
Gene:CYP24A1
Gene Synonym:
Exon (11)1756..3068
Gene:CYP24A1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001128915
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001128915

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCTCCC CCATCAGCAA GAGCCGCTCG CTTGCCGCCT TCCTGCAGCA GCTGCGCAGT 
CCGAGGCAGC CCCCGAGACT GGTGACATCT ACGGCGTACA CGTCCCCTCA GCCGCGAGAG
GTGCCAGTCT GCCCGCTGAC AGCTGGTGGC GAGACTCAGA ACGCGGCCGC CCTGCCGGGC
CCCACCAGCT GGCCACTGCT GGGCAGCCTG CTGCAGATTC TCTGGAAAGG GGGTCTCAAG
AAACAGCACG ACACCCTGGT GGAGTACCAC AAGAAGTATG GCAAGATTTT CCGCATGAAG
TTGGGTTCCT TTGAGTCGGT GCACCTGGGC TCGCCATGCC TGCTGGAAGC GCTGTACCGC
ACCGAGAGCG CGTACCCGCA GCGGCTGGAG ATCAAACCGT GGAAGGCCTA TCGCGACTAC
CGCAAAGAAG GCTACGGGCT GCTGATCCTG GAAGGGGAAG ACTGGCAGCG GGTCCGGAGT
GCCTTTCAAA AGAAACTAAT GAAACCAGGG GAAGTGATGA AGCTGGACAA CAAAATCAAT
GAGGTCTTGG CCGATTTTAT GGGCAGAATA GATGAGCTCT GTGATGAAAG AGGCCACGTT
GAAGACTTGT ACAGCGAACT GAACAAATGG TCGTTTGAAA GTATCTGCCT CGTGTTGTAT
GAGAAGAGAT TTGGGCTTCT CCAGAAGAAT GCAGGGGATG AAGCTGTGAA CTTCATCATG
GCCATCAAAA CAATGATGAG CACGTTTGGG AGGATGATGG TCACTCCAGT CGAGCTGCAC
AAGAGCCTCA ACACCAAGGT CTGGCAGGAC CACACTCTGG CCTGGGACAC CATTTTCAAA
TCAGTCAAAG CTTGTATCGA CAACCGGTTA GAGAAGTATT CTCAGCAGCC TAGTGCAGAT
TTCCTTTGTG ACATTTATCA CCAGAATCGG CTTTCAAAGA AAGAATTGTA TGCTGCTGTC
ACAGAGCTCC AGCTGGCTGC GGTGGAAACG ACAGCAAACA GTCTAATGTG GATTCTCTAC
AATTTATCCC GTAATCCCCA AGTGCAACAA AAGCTTCTTA AGGAAATTCA AAGTGTATTA
CCTGAGAATC AGGTGCCACG GGCAGAAGAT TTGAGGAATA TGCCGTATTT AAAAGCCTGT
CTGAAAGAAT CTATGAGGCT TACGCCGAGT GTACCATTTA CAACTCGGAC TCTTGACAAG
GCAACAGTTC TGGGTGAATA TGCTTTACCC AAAGGAATTG TCCGCAAATA CGACATCCAG
GCCACAGACA ATGAGCCTGT TGAGATGCTA CACTCAGGCA CCCTGGTGCC CAGCCGGGAA
CTCCCCATCG CGTTTTGCCA GCGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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