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CYP27B1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CYP27B1
Entrez Gene ID 1594
Full Name cytochrome P450, family 27, subfamily B, polypeptide 1
Synonyms CP2B, CYP1, CYP1alpha, CYP27B, P450c1, PDDR, VDD1, VDDR, VDDRI, VDR
General protein information
Preferred Names
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Names
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
1alpha(OH)ase
VD3 1A hydroxylase
cytochrome p450 27B1
cytochrome P450C1 alpha
cytochrome P450VD1-alpha
calcidiol 1-monooxygenase
25-OHD-1 alpha-hydroxylase
25 hydroxyvitamin D3-1-alpha hydroxylase
cytochrome P450 subfamily XXVIIB polypeptide 1
NP_000776.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q14.1

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609506

Disorder Html: Vitamin D-dependent rickets, type I, 264700 (3)





The following CYP27B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP27B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27370 NM_000785 Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$307.30
$439.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu27370
Accession Version NM_000785.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP296031.1, AB005038.1 and AW449645.1. This sequence is a reference standard in the RefSeqGene project. On Sep 2, 2005 this sequence version replaced gi:13904864. Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB005038.1, AF020192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGACCCAGA CCCTCAAGTA CGCCTCCAGA GTGTTCCATC GCGTCCGCTG GGCGCCCGAG 
TTGGGCGCCT CCCTAGGCTA CCGAGAGTAC CACTCAGCAC GCCGGAGCTT GGCAGACATC
CCAGGCCCCT CTACGCCCAG CTTTCTGGCC GAACTTTTCT GCAAGGGGGG GCTGTCGAGG
CTACACGAGC TGCAGGTGCA GGGCGCCGCG CACTTCGGGC CGGTGTGGCT AGCCAGCTTT
GGGACAGTGC GCACCGTGTA CGTGGCTGCC CCTGCACTCG TCGAGGAGCT GCTGCGACAG
GAGGGACCCC GGCCCGAGCG CTGCAGCTTC TCGCCCTGGA CGGAGCACCG CCGCTGCCGC
CAGCGGGCTT GCGGACTGCT CACTGCGGAA GGCGAAGAAT GGCAAAGGCT CCGCAGTCTC
CTGGCCCCGC TCCTCCTCCG GCCTCAAGCG GCCGCCCGCT ACGCCGGAAC CCTGAACAAC
GTAGTCTGCG ACCTTGTGCG GCGTCTGAGG CGCCAGCGGG GACGTGGCAC GGGGCCGCCC
GCCCTGGTTC GGGACGTGGC GGGGGAATTT TACAAGTTCG GACTGGAAGG CATCGCCGCG
GTTCTGCTCG GCTCGCGCTT GGGCTGCCTG GAGGCTCAAG TGCCACCCGA CACGGAGACC
TTCATCCGCG CTGTGGGCTC GGTGTTTGTG TCCACGCTGT TGACCATGGC GATGCCCCAC
TGGCTGCGCC ACCTTGTGCC TGGGCCCTGG GGCCGCCTCT GCCGAGACTG GGACCAGATG
TTTGCATTTG CTCAGAGGCA CGTGGAGCGG CGAGAGGCAG AGGCAGCCAT GAGGAACGGA
GGACAGCCCG AGAAGGACCT GGAGTCTGGG GCGCACCTGA CCCACTTCCT GTTCCGGGAA
GAGTTGCCTG CCCAGTCCAT CCTGGGAAAT GTGACAGAGT TGCTATTGGC GGGAGTGGAC
ACGGTGTCCA ACACGCTCTC TTGGGCTCTG TATGAGCTCT CCCGGCACCC CGAAGTCCAG
ACAGCACTCC ACTCAGAGAT CACAGCTGCC CTGAGCCCTG GCTCCAGTGC CTACCCCTCA
GCCACTGTTC TGTCCCAGCT GCCCCTGCTG AAGGCGGTGG TCAAGGAAGT GCTAAGACTG
TACCCTGTGG TACCTGGAAA TTCTCGTGTC CCAGACAAAG ACATTCATGT GGGTGACTAT
ATTATCCCCA AAAATACGCT GGTCACTCTG TGTCACTATG CCACTTCAAG GGACCCTGCC
CAGTTCCCAG AGCCAAATTC TTTTCGTCCA GCTCGCTGGC TGGGGGAGGG TCCCACCCCC
CACCCATTTG CATCTCTTCC CTTTGGCTTT GGCAAGCGCA GCTGTATGGG GAGACGCCTG
GCAGAGCTTG AATTGCAAAT GGCTTTGGCC CAGATCCTAA CACATTTTGA GGTGCAGCCT
GAGCCAGGTG CGGCCCCAGT TAGACCCAAG ACCCGGACTG TCCTGGTACC TGAAAGGAGC
ATCAACCTAC AGTTTTTGGA CAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000776.1
CDS153..1679
Misc Feature(1)105..107(+)
Misc Feature(2)273..1667(+)
Misc Feature(3)1515..1517(+)
Exon (1)1..347
Gene:CYP27B1
Gene Synonym:
Exon (2)348..538
Gene:CYP27B1
Gene Synonym:
Exon (3)539..741
Gene:CYP27B1
Gene Synonym:
Exon (4)742..942
Gene:CYP27B1
Gene Synonym:
Exon (5)943..1115
Gene:CYP27B1
Gene Synonym:
Exon (6)1116..1288
Gene:CYP27B1
Gene Synonym:
Exon (7)1289..1367
Gene:CYP27B1
Gene Synonym:
Exon (8)1368..1565
Gene:CYP27B1
Gene Synonym:
Exon (9)1566..2487
Gene:CYP27B1
Gene Synonym:
Translation
Position Chain Variation Link
36 36 a, g dbSNP:756890214
58 58 a, g dbSNP:562916792
102 102 a, c dbSNP:758021519
105 105 c, t dbSNP:745544786
107 107 -, atg dbSNP:371880778
112 112 c, g dbSNP:751115520
121 121 c, g, t dbSNP:541475094
122 122 c, g, t dbSNP:372422608
123 123 -, c dbSNP:763650706
123 123 a, c dbSNP:201190359
124 124 c, t dbSNP:755347721
128 128 c, t dbSNP:751953236
131 131 c, g dbSNP:146281435
132 132 a, c, g dbSNP:560778422
134 134 a, c, g, t dbSNP:765302413
138 138 a, t dbSNP:761803324
141 141 c, t dbSNP:776510825
143 143 a, g dbSNP:768601263
148 148 a, c, t dbSNP:775231921
158 158 c, g dbSNP:771825668
166 166 g, t dbSNP:745516871
169 169 a, g dbSNP:374801900
173 173 c, g dbSNP:778438734
174 174 a, g dbSNP:577501884
178 178 a, c dbSNP:748833551
182 182 a, g dbSNP:777477723
183 183 a, g dbSNP:755512223
192 192 c, t dbSNP:150648140
193 193 a, g dbSNP:372223837
194 194 c, t dbSNP:758703209
195 195 a, g dbSNP:202028173
199 199 g, t dbSNP:765408119
201 201 c, t dbSNP:761902845
206 206 a, g dbSNP:537715358
208 208 a, c dbSNP:764196493
210 210 a, c, g dbSNP:775461299
212 212 a, g dbSNP:771838478
218 218 a, c dbSNP:759285613
223 223 c, t dbSNP:774009424
227 227 a, g dbSNP:770470667
228 228 c, g dbSNP:748991170
230 230 c, t dbSNP:777419232
233 233 c, t dbSNP:769487954
234 234 a, c dbSNP:368820787
235 235 a, g dbSNP:780429096
240 240 g, t dbSNP:758749494
242 242 c, t dbSNP:750773217
251 251 a, g dbSNP:779382219
253 253 g, t dbSNP:757388190
254 254 c, g dbSNP:141890302
255 255 c, t dbSNP:754122776
269 269 c, t dbSNP:764141450
275 275 a, c dbSNP:760753732
282 282 c, t dbSNP:752594802
290 290 c, t dbSNP:772736324
297 297 c, g dbSNP:201343041
300 300 a, g dbSNP:759569512
302 302 c, g dbSNP:139558307
308 308 a, t dbSNP:770828560
316 316 a, t dbSNP:762682469
318 318 c, g, t dbSNP:202032208
319 319 c, g dbSNP:769363080
322 322 a, c, g, t dbSNP:376304260
323 323 -, g dbSNP:763437121
323 323 -, g dbSNP:767074885
326 326 a, g dbSNP:772434439
329 329 a, g dbSNP:746424645
334 334 c, t dbSNP:779138180
336 336 c, t dbSNP:757673918
341 341 a, g dbSNP:576826652
352 352 a, g dbSNP:548176807
359 359 c, t dbSNP:530008944
360 360 a, g dbSNP:761605625
368 368 c, t dbSNP:565490799
374 374 a, g dbSNP:547387731
384 384 a, g dbSNP:768276712
413 413 c, t dbSNP:532181970
414 414 -, g dbSNP:387906260
416 416 g, t dbSNP:746345633
431 431 c, t dbSNP:757689692
461 461 c, t dbSNP:774932003
462 462 c, t dbSNP:771215877
463 463 a, g dbSNP:370689065
467 467 c, t dbSNP:564954189
468 468 a, g dbSNP:756300813
472 472 a, g dbSNP:28934604
478 478 c, g dbSNP:748472549
480 480 c, t dbSNP:781119946
482 482 c, t dbSNP:546648396
489 489 c, t dbSNP:751386712
494 494 a, g dbSNP:766452283
498 498 c, g dbSNP:758218175
500 500 c, t dbSNP:372771210
502 502 a, g dbSNP:765161769
504 504 a, c dbSNP:761365991
508 508 a, g dbSNP:776399364
510 510 c, t dbSNP:763451961
513 513 c, t dbSNP:760233049
516 516 a, c dbSNP:561541172
522 522 g, t dbSNP:774677094
524 524 c, t dbSNP:771410002
526 526 a, g dbSNP:28934605
530 530 g, t dbSNP:542906068
537 537 a, g dbSNP:58915677
540 540 a, g dbSNP:577603625
548 548 a, g dbSNP:767032150
552 552 c, t dbSNP:777022503
557 557 a, t dbSNP:768968235
567 567 a, t dbSNP:760965198
569 569 c, t dbSNP:775865565
578 578 c, t dbSNP:772209037
590 590 c, t dbSNP:745971801
598 598 a, c dbSNP:778942273
600 600 a, g dbSNP:770873791
601 601 c, t dbSNP:749254542
605 605 c, t dbSNP:556146256
606 606 a, g dbSNP:777612495
608 608 c, t dbSNP:755878350
609 609 c, t dbSNP:747734371
610 610 g, t dbSNP:180727885
611 611 c, t dbSNP:7965963
613 613 a, t dbSNP:574099143
615 615 a, g dbSNP:754435549
617 617 c, t dbSNP:751048270
618 618 a, g dbSNP:765846783
619 619 a, g dbSNP:757777816
632 632 a, c, t dbSNP:141906701
639 639 c, t dbSNP:761123158
640 640 g, t dbSNP:775812483
641 641 a, c dbSNP:767869773
648 648 c, g dbSNP:8176344
652 652 c, g dbSNP:372846214
654 654 c, t dbSNP:369510241
659 659 g, t dbSNP:749194445
660 660 -, ag dbSNP:770729138
663 663 c, t dbSNP:535463729
670 670 a, g dbSNP:769593161
674 674 a, t dbSNP:565963580
680 680 a, c dbSNP:781014540
682 682 c, t dbSNP:754595210
683 683 a, g, t dbSNP:143165542
686 686 a, g dbSNP:757859131
692 692 c, t dbSNP:200761229
693 693 g, t dbSNP:368126466
695 695 c, g, t dbSNP:762932963
697 697 c, t dbSNP:753086020
702 702 c, t dbSNP:554101223
703 703 a, g dbSNP:759821430
705 705 a, g dbSNP:774455044
708 708 a, g dbSNP:766418508
712 712 c, g dbSNP:775399881
713 713 a, g, t dbSNP:773153213
718 718 a, g dbSNP:118204012
720 720 c, t dbSNP:375234468
743 743 a, c dbSNP:776560698
744 744 a, g dbSNP:768373645
746 746 c, g dbSNP:549824639
753 753 a, g, t dbSNP:771598984
756 756 c, g dbSNP:745571910
762 762 c, g, t dbSNP:770581711
766 766 c, t dbSNP:748602968
767 767 a, g, t dbSNP:572765133
770 770 a, c dbSNP:370743468
774 774 a, g dbSNP:751848951
778 778 c, g dbSNP:201503366
779 779 c, t dbSNP:758546382
782 782 -, g dbSNP:387906258
794 794 -, gccac dbSNP:772171082
803 803 a, c dbSNP:769953744
808 808 -, a dbSNP:778534360
819 819 c, t dbSNP:149518825
821 821 a, c dbSNP:765241743
822 822 g, t dbSNP:367743385
830 830 c, t dbSNP:753733417
833 833 a, g dbSNP:764038309
842 842 a, g dbSNP:61734540
844 844 -, c dbSNP:387906259
856 856 a, c dbSNP:775114472
858 858 a, c, g dbSNP:373542360
863 863 a, g dbSNP:759056278
869 869 a, c dbSNP:774153859
872 872 c, t dbSNP:770528985
874 874 g, t dbSNP:748833715
879 879 c, t dbSNP:772898470
880 880 a, g dbSNP:769013170
883 883 a, g, t dbSNP:780269626
885 885 c, t dbSNP:758802757
887 887 c, t dbSNP:746021709
894 894 a, g dbSNP:779137478
896 896 a, g dbSNP:745908985
905 905 c, g dbSNP:757277620
912 912 c, t dbSNP:753888767
915 915 c, g dbSNP:374481894
916 916 a, g dbSNP:190470241
918 918 a, g dbSNP:200174985
931 931 g, t dbSNP:752696414
953 953 c, t dbSNP:150334927
959 959 c, g dbSNP:141241519
961 961 a, g dbSNP:762684209
994 994 c, g dbSNP:750115347
997 997 a, t dbSNP:764582398
999 999 c, t dbSNP:370850634
1001 1001 c, t dbSNP:775810183
1008 1008 a, g dbSNP:772608705
1012 1012 g, t dbSNP:760071258
1017 1017 c, t dbSNP:774690200
1018 1018 c, t dbSNP:771340739
1024 1024 a, c dbSNP:749448463
1025 1025 a, g dbSNP:778091400
1048 1048 g, t dbSNP:551616538
1054 1054 a, t dbSNP:748241510
1055 1055 c, g dbSNP:377397604
1059 1059 c, t dbSNP:754963151
1062 1062 a, g dbSNP:751606502
1066 1066 a, c dbSNP:567237244
1069 1069 c, g dbSNP:781498062
1075 1075 c, t dbSNP:758271238
1078 1078 a, g dbSNP:750002391
1085 1085 c, g dbSNP:200567715
1094 1094 a, g dbSNP:8176345
1097 1097 a, g dbSNP:753354343
1098 1098 c, t dbSNP:763656534
1106 1106 a, g dbSNP:760020111
1107 1107 c, g dbSNP:774849834
1114 1114 c, g dbSNP:118204007
1115 1115 g, t dbSNP:201302886
1119 1119 g, t dbSNP:750908103
1123 1123 a, g dbSNP:765572208
1126 1126 c, g dbSNP:762118198
1137 1137 g, t dbSNP:776942384
1139 1139 c, t dbSNP:764387303
1142 1142 a, c, g dbSNP:775708027
1149 1149 c, t dbSNP:145790221
1151 1151 a, c dbSNP:772097552
1155 1155 c, t dbSNP:745757851
1156 1156 a, c, g dbSNP:28934606
1161 1161 c, t dbSNP:201819682
1162 1162 a, c dbSNP:141927200
1163 1163 c, t dbSNP:749017261
1164 1164 a, g dbSNP:777588673
1172 1172 a, g dbSNP:578010655
1179 1179 c, t dbSNP:118204011
1187 1187 a, g dbSNP:372527928
1192 1192 a, t dbSNP:556530774
1195 1195 c, t dbSNP:762823552
1196 1196 a, g dbSNP:780623929
1201 1201 c, t dbSNP:754452515
1204 1204 a, g, t dbSNP:537825066
1209 1209 c, g, t dbSNP:151335249
1211 1211 g, t dbSNP:567195245
1217 1217 c, t dbSNP:191727255
1219 1219 a, g dbSNP:13377933
1220 1220 a, t dbSNP:752977580
1222 1222 c, g dbSNP:142446425
1223 1223 c, t dbSNP:759563696
1225 1225 a, c dbSNP:774541604
1230 1230 c, t dbSNP:770746377
1238 1238 a, t dbSNP:373672754
1239 1239 a, g dbSNP:772982189
1246 1246 c, t dbSNP:769591385
1251 1251 c, g dbSNP:747732728
1256 1256 c, t dbSNP:780857122
1260 1260 a, c dbSNP:753298199
1268 1268 a, g dbSNP:199575147
1271 1271 a, g dbSNP:148336763
1272 1272 a, g dbSNP:779651723
1273 1273 c, t dbSNP:2229103
1281 1281 a, g dbSNP:754449343
1292 1292 a, g dbSNP:776536884
1296 1296 c, t dbSNP:28934607
1315 1315 a, c dbSNP:768158988
1317 1317 c, g, t dbSNP:118204010
1318 1318 a, g dbSNP:118204009
1329 1329 a, g dbSNP:771720639
1332 1332 c, g dbSNP:749745310
1334 1334 c, t dbSNP:145339059
1337 1337 g, t dbSNP:756495841
1341 1341 a, g dbSNP:748494534
1343 1343 a, c, g dbSNP:755142592
1347 1347 a, g dbSNP:751820138
1348 1348 a, g dbSNP:766306920
1349 1349 c, t dbSNP:758508383
1351 1351 a, g dbSNP:750314936
1363 1363 a, g dbSNP:765139748
1369 1369 c, t dbSNP:758671027
1370 1370 a, g dbSNP:750468088
1374 1374 g, t dbSNP:377154743
1378 1378 c, t dbSNP:118204008
1388 1388 a, c dbSNP:78250220
1396 1396 c, t dbSNP:753823609
1400 1400 a, g dbSNP:374454037
1403 1403 g, t dbSNP:760548148
1406 1406 a, c, t dbSNP:769534483
1407 1407 a, c dbSNP:767253913
1418 1418 c, t dbSNP:759331498
1420 1420 c, t dbSNP:773986669
1432 1432 a, c dbSNP:762701380
1438 1438 a, g dbSNP:568165874
1444 1444 c, t dbSNP:556493554
1447 1447 a, g dbSNP:768857789
1455 1455 a, g dbSNP:544219775
1457 1457 a, g dbSNP:780440003
1462 1462 g, t dbSNP:150025067
1463 1463 g, t dbSNP:746144650
1469 1469 c, g dbSNP:778903870
1471 1471 c, g dbSNP:757358360
1472 1472 c, t dbSNP:753678470
1473 1473 c, g dbSNP:573538895
1477 1477 -, cccaccc dbSNP:780950819
1484 1484 a, g dbSNP:756054548
1505 1505 c, g dbSNP:752504237
1509 1509 c, t dbSNP:767480544
1510 1510 a, g dbSNP:759208930
1514 1514 c, t dbSNP:751262543
1516 1516 a, g dbSNP:765035904
1525 1525 -, gggag dbSNP:754559171
1527 1527 c, t dbSNP:555068245
1528 1528 a, g dbSNP:762550487
1529 1529 a, c dbSNP:772650262
1532 1532 a, g dbSNP:199923824
1555 1555 c, t dbSNP:761149528
1557 1557 a, t dbSNP:549873942
1568 1568 c, t dbSNP:749948148
1575 1575 c, t dbSNP:201869290
1583 1583 a, c, g dbSNP:374681375
1591 1591 c, g dbSNP:761183667
1592 1592 c, t dbSNP:775976633
1594 1594 a, g dbSNP:772383081
1603 1603 c, t dbSNP:759915706
1604 1604 a, g dbSNP:8176349
1618 1618 c, g dbSNP:751062287
1619 1619 a, c dbSNP:771151644
1626 1626 c, t dbSNP:749537609
1627 1627 a, c, g dbSNP:773538942
1628 1628 g, t dbSNP:748058985
1631 1631 a, t dbSNP:191239138
1651 1651 a, g dbSNP:371735439
1655 1655 a, c dbSNP:746773160
1657 1657 a, g dbSNP:201629800
1662 1662 c, t dbSNP:758053526
1664 1664 a, g dbSNP:374536920
1681 1681 c, t dbSNP:764734028
1683 1683 c, g dbSNP:756788811
1686 1686 a, g dbSNP:753152890
1687 1687 a, g dbSNP:143020052
1693 1693 g, t dbSNP:767989731
1697 1697 a, g dbSNP:372269276
1705 1705 -, tt dbSNP:750827656
1706 1706 -, acc dbSNP:765532756
1711 1711 g, t dbSNP:41325246
1716 1716 c, t dbSNP:371594650
1718 1718 a, g dbSNP:763171658
1724 1724 a, g dbSNP:779510099
1755 1755 a, g dbSNP:147119803
1756 1756 a, g dbSNP:8176350
1783 1783 c, g dbSNP:539158689
1822 1822 a, g dbSNP:569065283
1883 1883 a, g dbSNP:542717895
1894 1894 c, g dbSNP:557294448
1926 1926 c, g dbSNP:780627307
1940 1940 a, t dbSNP:536111659
1943 1943 g, t dbSNP:568645777
2024 2024 c, t dbSNP:141820034
2087 2087 a, g dbSNP:528489670
2093 2093 c, t dbSNP:570897771
2094 2094 a, g dbSNP:8176351
2105 2105 c, t dbSNP:751250622
2125 2125 a, g dbSNP:763711308
2131 2131 a, c dbSNP:758207671
2155 2155 g, t dbSNP:563318747
2260 2260 a, c dbSNP:752395227
2273 2273 c, t dbSNP:562987203
2277 2277 a, g dbSNP:531029924
2280 2280 c, t dbSNP:562671945
2281 2281 a, c dbSNP:180865889
2290 2290 c, t dbSNP:529118144
2313 2313 c, t dbSNP:765222801
2314 2314 a, g dbSNP:533718329
2378 2378 a, c dbSNP:189295818
2380 2380 c, t dbSNP:572466704
2410 2410 c, t dbSNP:563744441
2417 2417 c, t dbSNP:545464947
2435 2435 -, a dbSNP:34824141
2469 2469 g, t dbSNP:575259227

Target ORF information:

RefSeq Version NM_000785
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000785

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGACCCAGA CCCTCAAGTA CGCCTCCAGA GTGTTCCATC GCGTCCGCTG GGCGCCCGAG 
TTGGGCGCCT CCCTAGGCTA CCGAGAGTAC CACTCAGCAC GCCGGAGCTT GGCAGACATC
CCAGGCCCCT CTACGCCCAG CTTTCTGGCC GAACTTTTCT GCAAGGGGGG GCTGTCGAGG
CTACACGAGC TGCAGGTGCA GGGCGCCGCG CACTTCGGGC CGGTGTGGCT AGCCAGCTTT
GGGACAGTGC GCACCGTGTA CGTGGCTGCC CCTGCACTCG TCGAGGAGCT GCTGCGACAG
GAGGGACCCC GGCCCGAGCG CTGCAGCTTC TCGCCCTGGA CGGAGCACCG CCGCTGCCGC
CAGCGGGCTT GCGGACTGCT CACTGCGGAA GGCGAAGAAT GGCAAAGGCT CCGCAGTCTC
CTGGCCCCGC TCCTCCTCCG GCCTCAAGCG GCCGCCCGCT ACGCCGGAAC CCTGAACAAC
GTAGTCTGCG ACCTTGTGCG GCGTCTGAGG CGCCAGCGGG GACGTGGCAC GGGGCCGCCC
GCCCTGGTTC GGGACGTGGC GGGGGAATTT TACAAGTTCG GACTGGAAGG CATCGCCGCG
GTTCTGCTCG GCTCGCGCTT GGGCTGCCTG GAGGCTCAAG TGCCACCCGA CACGGAGACC
TTCATCCGCG CTGTGGGCTC GGTGTTTGTG TCCACGCTGT TGACCATGGC GATGCCCCAC
TGGCTGCGCC ACCTTGTGCC TGGGCCCTGG GGCCGCCTCT GCCGAGACTG GGACCAGATG
TTTGCATTTG CTCAGAGGCA CGTGGAGCGG CGAGAGGCAG AGGCAGCCAT GAGGAACGGA
GGACAGCCCG AGAAGGACCT GGAGTCTGGG GCGCACCTGA CCCACTTCCT GTTCCGGGAA
GAGTTGCCTG CCCAGTCCAT CCTGGGAAAT GTGACAGAGT TGCTATTGGC GGGAGTGGAC
ACGGTGTCCA ACACGCTCTC TTGGGCTCTG TATGAGCTCT CCCGGCACCC CGAAGTCCAG
ACAGCACTCC ACTCAGAGAT CACAGCTGCC CTGAGCCCTG GCTCCAGTGC CTACCCCTCA
GCCACTGTTC TGTCCCAGCT GCCCCTGCTG AAGGCGGTGG TCAAGGAAGT GCTAAGACTG
TACCCTGTGG TACCTGGAAA TTCTCGTGTC CCAGACAAAG ACATTCATGT GGGTGACTAT
ATTATCCCCA AAAATACGCT GGTCACTCTG TGTCACTATG CCACTTCAAG GGACCCTGCC
CAGTTCCCAG AGCCAAATTC TTTTCGTCCA GCTCGCTGGC TGGGGGAGGG TCCCACCCCC
CACCCATTTG CATCTCTTCC CTTTGGCTTT GGCAAGCGCA GCTGTATGGG GAGACGCCTG
GCAGAGCTTG AATTGCAAAT GGCTTTGGCC CAGATCCTAA CACATTTTGA GGTGCAGCCT
GAGCCAGGTG CGGCCCCAGT TAGACCCAAG ACCCGGACTG TCCTGGTACC TGAAAGGAGC
ATCAACCTAC AGTTTTTGGA CAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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