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SPRED1 sprouty-related, EVH1 domain containing 1 [Homo sapiens (human)]

Gene Symbol SPRED1
Entrez Gene ID 161742
Full Name sprouty-related, EVH1 domain containing 1
Synonyms NFLS, PPP1R147, hSpred1, spred-1
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q14

Summary The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609291

Disorder Html: Legius syndrome, 611431 (3)





The following SPRED1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRED1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu48143 XM_005254202 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu77141 XM_011521288 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu77141 XM_011521289 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu48144 XM_005254203 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu77141 XM_011521290 PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu18529 NM_152594 Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu48143
Accession Version XM_005254202.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530405472. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005254259.1
CDS1100..2470
Misc Feature(1)1172..1510(+)
Misc Feature(2)1199..1408(+)
Misc Feature(3)2129..2419(+)
Translation
Position Chain Variation Link
30 30 c, t dbSNP:578109576
31 31 c, t dbSNP:753674221
33 33 c, g dbSNP:545234930
41 41 -, a dbSNP:754835889
42 42 -, a dbSNP:778544680
94 94 a, c dbSNP:753013391
107 107 a, g dbSNP:563464823
115 115 a, g dbSNP:530873122
145 145 c, t dbSNP:758605846
178 178 -, gct dbSNP:758522996
180 180 g, t dbSNP:1522781
232 232 a, g dbSNP:560973597
244 244 c, g dbSNP:528091119
254 254 a, g dbSNP:546545058
303 303 a, g dbSNP:1522782
432 432 c, t dbSNP:193069101
443 443 -, t dbSNP:572126972
483 483 c, t dbSNP:551597076
544 544 c, g, t dbSNP:569895675
552 552 a, g dbSNP:184673190
674 674 c, t dbSNP:189438859
685 685 c, t dbSNP:567923633
755 755 g, t dbSNP:534711799
768 768 a, g dbSNP:147075186
791 791 c, t dbSNP:578173447
835 835 a, t dbSNP:746319268
856 856 c, t dbSNP:138385793
866 866 c, g dbSNP:557220249
899 899 a, g dbSNP:770231868
909 909 c, t dbSNP:556907443
934 934 a, g dbSNP:542772692
938 938 -, g dbSNP:531519324
963 963 a, c dbSNP:561044956
964 964 c, t dbSNP:573042758
1007 1007 c, t dbSNP:192699510
1049 1049 c, g dbSNP:369369431
1062 1062 g, t dbSNP:762837345
1070 1070 a, g dbSNP:564526874
1072 1072 c, t dbSNP:751783011
1076 1076 a, g dbSNP:755230054
1082 1082 g, t dbSNP:755489788
1083 1083 a, t dbSNP:781241994
1086 1086 a, c dbSNP:532105923
1087 1087 c, g dbSNP:756552268
1088 1088 c, g, t dbSNP:371200183
1098 1098 a, t dbSNP:773950720
1105 1105 c, t dbSNP:775693385
1125 1125 a, t dbSNP:200157475
1126 1126 c, t dbSNP:727504717
1127 1127 a, t dbSNP:779189071
1129 1129 a, c dbSNP:201692618
1140 1140 a, g dbSNP:748512713
1159 1159 -, t dbSNP:767219677
1160 1160 c, t dbSNP:79328043
1177 1177 c, t dbSNP:764234046
1178 1178 a, g dbSNP:753924528
1182 1182 a, g, t dbSNP:757726772
1187 1187 c, g dbSNP:750777752
1188 1188 a, g dbSNP:758855467
1193 1193 c, g dbSNP:780614363
1195 1195 c, g dbSNP:367745143
1204 1204 c, t dbSNP:755788325
1205 1205 c, t dbSNP:121434313
1254 1254 a, c, g dbSNP:781390048
1258 1258 c, t dbSNP:748759533
1259 1259 a, g dbSNP:147204964
1266 1266 a, t dbSNP:121434318
1269 1269 g, t dbSNP:778847063
1276 1276 c, t dbSNP:745859168
1278 1278 c, t dbSNP:771825294
1283 1283 c, t dbSNP:148646547
1298 1298 c, t dbSNP:775334473
1303 1303 g, t dbSNP:760939387
1307 1307 a, t dbSNP:768775931
1312 1312 c, t dbSNP:397517871
1313 1313 a, g dbSNP:761830220
1316 1316 c, t dbSNP:765788978
1317 1317 a, g, t dbSNP:750686148
1325 1325 c, t dbSNP:121434315
1326 1326 a, g dbSNP:372205720
1331 1331 a, c dbSNP:751923342
1346 1346 a, g dbSNP:369150309
1363 1363 a, t dbSNP:763749955
1364 1364 a, t dbSNP:727504170
1405 1405 c, g, t dbSNP:773943895
1407 1407 a, g dbSNP:756975029
1414 1414 a, g dbSNP:778384985
1415 1415 a, g dbSNP:749950643
1416 1416 c, t dbSNP:761473300
1425 1425 a, g dbSNP:556636985
1426 1426 a, g dbSNP:7182445
1440 1440 c, t dbSNP:754706111
1441 1441 a, g dbSNP:372791883
1446 1446 a, g dbSNP:748376033
1456 1456 a, t dbSNP:770197803
1483 1483 c, t dbSNP:773363523
1484 1484 c, t dbSNP:121434312
1485 1485 a, g dbSNP:749378713
1488 1488 a, g dbSNP:373381933
1493 1493 a, g dbSNP:774740245
1506 1506 c, t dbSNP:760132893
1508 1508 c, t dbSNP:767771754
1519 1519 c, t dbSNP:761058615
1520 1520 a, g dbSNP:764823722
1523 1523 c, t dbSNP:149883080
1531 1531 c, t dbSNP:772830013
1535 1535 a, g dbSNP:755991015
1536 1536 c, g dbSNP:762633609
1540 1540 a, g dbSNP:778868909
1543 1543 g, t dbSNP:765800186
1548 1548 a, g dbSNP:751138842
1552 1552 c, t dbSNP:12148911
1559 1559 g, t dbSNP:763971890
1561 1561 a, g dbSNP:753701682
1573 1573 c, t dbSNP:757357920
1581 1581 a, g dbSNP:373384814
1586 1586 c, g dbSNP:750421037
1598 1598 c, g, t dbSNP:758483715
1600 1600 c, t dbSNP:376944837
1605 1605 a, t dbSNP:747508368
1611 1611 a, c dbSNP:769206547
1612 1612 a, t dbSNP:781477854
1630 1630 g, t dbSNP:748550020
1633 1633 c, g dbSNP:770446191
1638 1638 a, t dbSNP:774070689
1642 1642 a, c dbSNP:759158378
1645 1645 c, t dbSNP:771673657
1646 1646 c, t dbSNP:376527959
1654 1654 a, g dbSNP:532147114
1659 1659 -, ctt dbSNP:763365429
1665 1665 c, t dbSNP:553832516
1670 1670 a, g dbSNP:143121426
1695 1695 a, g dbSNP:764135694
1696 1696 c, t dbSNP:201335683
1698 1698 c, t dbSNP:370735097
1699 1699 a, g dbSNP:202092705
1700 1700 a, c dbSNP:200142785
1702 1702 a, c, t dbSNP:764990934
1705 1705 c, t dbSNP:758617124
1713 1713 a, g dbSNP:766431294
1722 1722 c, t dbSNP:147474792
1729 1729 c, t dbSNP:756643495
1734 1734 c, t dbSNP:778144652
1742 1742 a, g dbSNP:139897054
1745 1745 a, g dbSNP:758087535
1749 1749 a, g dbSNP:779879921
1753 1753 c, g dbSNP:746708059
1754 1754 a, c dbSNP:368660900
1759 1759 c, t dbSNP:776073446
1768 1768 c, t dbSNP:149991304
1769 1769 a, g dbSNP:769630742
1772 1772 c, t dbSNP:121434316
1775 1775 a, c, t dbSNP:376699107
1776 1776 a, g dbSNP:371274407
1778 1778 c, g, t dbSNP:121434314
1780 1780 a, c dbSNP:144227155
1798 1798 a, g dbSNP:766539664
1804 1804 a, g dbSNP:774530752
1810 1810 c, g, t dbSNP:144764225
1820 1820 g, t dbSNP:201837340
1822 1822 a, c dbSNP:376226762
1831 1831 a, g dbSNP:764287445
1837 1837 c, g dbSNP:138553244
1847 1847 a, g dbSNP:762478369
1866 1866 a, g dbSNP:766152186
1900 1900 a, g dbSNP:765684707
1905 1905 a, g dbSNP:750832911
1919 1919 a, t dbSNP:121434317
1930 1930 c, t dbSNP:116667267
1956 1956 a, g dbSNP:111363743
1958 1958 g, t dbSNP:780737673
1970 1970 a, g dbSNP:752201824
1976 1976 c, t dbSNP:755557783
1989 1989 c, t dbSNP:759197561
1999 1999 a, c dbSNP:777379558
2001 2001 -, aatcagact dbSNP:778423017
2001 2001 a, c dbSNP:372665535
2010 2010 a, g dbSNP:201933663
2014 2014 a, g dbSNP:535687467
2021 2021 c, t dbSNP:554327738
2022 2022 a, g dbSNP:200974459
2023 2023 c, t dbSNP:775556490
2027 2027 c, g dbSNP:761001792
2034 2034 c, g dbSNP:540124243
2041 2041 a, g dbSNP:776909752
2055 2055 a, g dbSNP:764868020
2057 2057 a, g, t dbSNP:765841849
2061 2061 c, t dbSNP:114636635
2063 2063 a, g dbSNP:752191960
2066 2066 c, t dbSNP:375700452
2073 2073 c, t dbSNP:766814966
2074 2074 a, g, t dbSNP:140644874
2079 2079 c, t dbSNP:115440602
2089 2089 a, g dbSNP:753357510
2098 2098 -, gaagtc dbSNP:749692794
2098 2098 a, g dbSNP:369711772
2112 2112 c, g dbSNP:750890621
2120 2120 a, c, g dbSNP:756634323
2129 2129 c, t dbSNP:758102077
2130 2130 a, g dbSNP:779966392
2135 2135 c, t dbSNP:765603808
2136 2136 a, g dbSNP:146702985
2140 2140 c, t dbSNP:768937237
2141 2141 a, g dbSNP:373477920
2166 2166 a, g dbSNP:376679136
2170 2170 a, g dbSNP:770375243
2172 2172 a, g dbSNP:773833198
2179 2179 c, g, t dbSNP:3751526
2180 2180 a, c, t dbSNP:774835982
2208 2208 c, g dbSNP:760186912
2211 2211 a, t dbSNP:763738243
2213 2213 a, g dbSNP:374685699
2215 2215 a, g dbSNP:749654043
2221 2221 c, t dbSNP:756739740
2222 2222 a, g dbSNP:764768156
2224 2224 a, g dbSNP:140225135
2231 2231 a, g dbSNP:372623722
2240 2240 a, t dbSNP:779660163
2243 2243 c, t dbSNP:397517870
2263 2263 g, t dbSNP:746827242
2282 2282 c, t dbSNP:755208632
2285 2285 -, g dbSNP:771343745
2305 2305 c, t dbSNP:781585106
2311 2311 a, g dbSNP:755223172
2321 2321 a, g dbSNP:769959530
2323 2323 c, t dbSNP:773409045
2326 2326 c, t dbSNP:749745659
2327 2327 a, g dbSNP:771480941
2329 2329 -, c dbSNP:774533789
2337 2337 g, t dbSNP:779058019
2339 2339 c, t dbSNP:759969513
2343 2343 a, g dbSNP:772476060
2345 2345 g, t dbSNP:776235336
2351 2351 a, g dbSNP:761520561
2352 2352 c, t dbSNP:748390368
2357 2357 g, t dbSNP:150307819
2360 2360 a, g, t dbSNP:762735151
2361 2361 c, t dbSNP:751260227
2374 2374 c, t dbSNP:754794684
2395 2395 c, t dbSNP:202087411
2396 2396 a, g dbSNP:752958999
2408 2408 a, g dbSNP:368307475
2418 2418 a, g dbSNP:369492789
2424 2424 c, g dbSNP:749389617
2430 2430 c, t dbSNP:200871227
2433 2433 a, g dbSNP:779454951
2436 2436 g, t dbSNP:746414913
2442 2442 a, g dbSNP:772421738
2448 2448 g, t dbSNP:573603750
2454 2454 -, at dbSNP:759838497
2459 2459 a, g dbSNP:761401264
2472 2472 a, c dbSNP:769445247
2503 2503 -, ata dbSNP:772254530
2517 2517 c, t dbSNP:772560499
2518 2518 a, g dbSNP:373694127
2534 2534 c, t dbSNP:544137820
2536 2536 a, g dbSNP:533114849
2551 2551 c, t dbSNP:773453342
2575 2575 a, g dbSNP:545295358
2613 2613 a, c dbSNP:770816015
2619 2619 a, g dbSNP:776485386
2638 2638 c, t dbSNP:192826011
2643 2643 c, t dbSNP:765093939
2653 2653 a, g dbSNP:530541798
2660 2660 a, g dbSNP:377091058
2675 2675 a, g dbSNP:567404158
2696 2696 a, g dbSNP:377201217
2718 2718 c, t dbSNP:16966842
2720 2720 a, g dbSNP:547722924
2726 2726 c, t dbSNP:145373657
2735 2735 a, c dbSNP:539607759
2791 2791 a, g dbSNP:137920898
2828 2828 c, g dbSNP:569990101
2829 2829 a, g dbSNP:537079236
2830 2830 a, g dbSNP:751117768
2852 2852 c, g dbSNP:13329392
2861 2861 g, t dbSNP:555317480
2897 2897 a, t dbSNP:185290497
2915 2915 a, g dbSNP:577788958
2925 2925 c, g dbSNP:545070531
3000 3000 a, g dbSNP:756612869
3005 3005 a, g dbSNP:370660664
3034 3034 a, t dbSNP:373626511
3048 3048 -, at dbSNP:147547509
3049 3049 -, atat dbSNP:759359048
3051 3051 c, t dbSNP:541503324
3060 3060 a, c dbSNP:553150319
3062 3062 a, c dbSNP:754213247
3064 3064 a, c dbSNP:577769507
3103 3103 a, g dbSNP:118146919
3132 3132 a, g dbSNP:374443282
3135 3135 c, t dbSNP:755236404
3189 3189 a, g dbSNP:563589753
3206 3206 a, t dbSNP:188754645
3226 3226 c, t dbSNP:530810790
3228 3228 c, t dbSNP:560005063
3253 3253 c, t dbSNP:770577944
3306 3306 a, g dbSNP:192636064
3339 3339 -, att dbSNP:541409661
3363 3363 c, t dbSNP:542673653
3370 3370 c, t dbSNP:560864932
3413 3413 g, t dbSNP:183715838
3510 3510 a, g dbSNP:115568221
3529 3529 -, a dbSNP:34079057
3576 3576 c, t dbSNP:571210831
3584 3584 g, t dbSNP:141742028
3662 3662 c, t dbSNP:28536719
3664 3664 c, t dbSNP:28576519
3695 3695 c, t dbSNP:551528743
3747 3747 c, g dbSNP:779337443
3768 3768 a, g dbSNP:376795432
3778 3778 a, g dbSNP:368946709
3807 3807 a, c dbSNP:570038934
3841 3841 c, t dbSNP:373111945
3893 3893 a, g dbSNP:748337294
3922 3922 a, c dbSNP:751531938
3960 3960 g, t dbSNP:555380695
3962 3962 g, t dbSNP:199891616
3984 3984 g, t dbSNP:376829790
3986 3986 a, g dbSNP:757780122
4127 4127 -, ctat dbSNP:146972863
4129 4129 -, atct dbSNP:57589132
4129 4129 a, g dbSNP:113071870
4190 4190 c, t dbSNP:567659062
4194 4194 a, c dbSNP:1554527
4203 4203 a, g dbSNP:777972348
4213 4213 c, t dbSNP:747157552
4217 4217 a, c dbSNP:561525888
4231 4231 c, t dbSNP:553453653
4236 4236 -, aag dbSNP:546611820
4279 4279 a, g dbSNP:578008401
4282 4282 a, g dbSNP:770892084
4307 4307 a, g dbSNP:778489832
4365 4365 c, t dbSNP:538871697
4367 4367 c, t dbSNP:776375597
4488 4488 g, t dbSNP:188392935
4513 4513 a, g dbSNP:180946373
4550 4550 c, g dbSNP:759262779
4683 4683 c, t dbSNP:542935372
4717 4717 a, g dbSNP:118057877
4792 4792 a, g dbSNP:747836383
4847 4847 a, g dbSNP:572802254
4882 4882 -, t dbSNP:532033889
4890 4890 c, t dbSNP:184024152
4955 4955 a, g dbSNP:565009702
4956 4956 a, t dbSNP:374589452
4972 4972 -, tgagtaaa dbSNP:770258605
4972 4972 g, t dbSNP:532084025
5033 5033 -, at dbSNP:373432788
5040 5040 c, t dbSNP:111997668
5068 5068 a, c dbSNP:189861748
5070 5070 c, t dbSNP:775287695
5075 5075 c, t dbSNP:530967462
5081 5081 c, t dbSNP:181716461
5082 5082 a, g dbSNP:567834681
5092 5092 a, g dbSNP:138645237
5105 5105 c, g dbSNP:546662922
5156 5156 a, t dbSNP:571741901
5179 5179 c, t dbSNP:539133361
5180 5180 g, t dbSNP:557234725
5193 5193 c, t dbSNP:762569457
5198 5198 -, t dbSNP:773860286
5203 5203 a, t dbSNP:763774696
5204 5204 c, g dbSNP:773847026
5212 5212 g, t dbSNP:149336440
5222 5222 -, g dbSNP:746468942
5256 5256 c, g dbSNP:147428585
5294 5294 c, g dbSNP:761286407
5301 5301 a, g dbSNP:35490364
5318 5318 c, t dbSNP:754227753
5323 5323 a, g dbSNP:187134665
5355 5355 a, g dbSNP:79309779
5378 5378 c, t dbSNP:765645509
5414 5414 a, t dbSNP:759460441
5416 5416 a, c dbSNP:564720434
5470 5470 g, t dbSNP:753155345
5499 5499 -, gctgaaa dbSNP:553420659
5527 5527 a, g dbSNP:72725362
5537 5537 -, tt dbSNP:771536852
5548 5548 g, t dbSNP:543909086
5581 5581 c, t dbSNP:111766079
5601 5601 c, t dbSNP:373027564
5608 5608 a, g dbSNP:531030936
5634 5634 a, g dbSNP:191914374
5662 5662 a, g dbSNP:566065329
5724 5724 a, g dbSNP:561659585
5731 5731 c, t dbSNP:751824076
5783 5783 a, c, t dbSNP:536604218
5785 5785 a, g dbSNP:757217761
5793 5793 c, t dbSNP:547019744
5801 5801 a, g dbSNP:781366830
5810 5810 a, g dbSNP:571542327
5864 5864 c, t dbSNP:548164209
5865 5865 a, g dbSNP:140749542
5885 5885 a, c dbSNP:551099276
5929 5929 a, g dbSNP:764169369
5954 5954 -, tt dbSNP:774937535
5991 5991 a, g dbSNP:11634702
6022 6022 a, g dbSNP:376186397
6030 6030 a, g dbSNP:200294834
6031 6031 a, g dbSNP:181980647
6042 6042 a, g dbSNP:113753698
6051 6051 g, t dbSNP:779791635
6053 6053 a, c, g dbSNP:566802137
6061 6061 c, t dbSNP:147489619
6067 6067 -, ta dbSNP:574998297
6081 6081 a, g dbSNP:558773794
6093 6093 g, t dbSNP:148525432
6097 6097 -, a dbSNP:546844553
6115 6115 -, g dbSNP:763956861
6117 6117 g, t dbSNP:186366365
6127 6127 c, t dbSNP:761527047
6168 6168 g, t dbSNP:76527876
6171 6171 a, g dbSNP:574179000
6196 6196 a, g dbSNP:543233753
6215 6215 a, g dbSNP:142928983
6258 6258 c, g dbSNP:577731830
6260 6260 c, t dbSNP:528822175
6275 6275 a, t dbSNP:759931845
6300 6300 a, g dbSNP:8039150
6302 6302 a, g dbSNP:565528602
6311 6311 g, t dbSNP:532564269
6362 6362 g, t dbSNP:112221665
6416 6416 a, g dbSNP:8039337
6466 6466 a, g dbSNP:72725364
6512 6512 a, c dbSNP:189589145
6547 6547 a, t dbSNP:572054998
6571 6571 a, g dbSNP:182237934
6572 6572 c, t dbSNP:577333581
6606 6606 -, att dbSNP:369376164
6637 6637 a, g dbSNP:758092431
6664 6664 a, t dbSNP:777252680
6676 6676 a, g dbSNP:746558881
6701 6701 a, g dbSNP:533950389
6707 6707 a, g dbSNP:558595140
6711 6711 c, g dbSNP:570386455
6734 6734 -, a dbSNP:542372343
6752 6752 -, ggtat dbSNP:767679657
6774 6774 a, g dbSNP:537477031
6818 6818 c, t dbSNP:375141692
6842 6842 a, g dbSNP:187788660
6846 6846 g, t dbSNP:781111622
6904 6904 c, t dbSNP:750554498
6906 6906 a, g dbSNP:191601293
6921 6921 -, at dbSNP:761502926
6941 6941 a, c dbSNP:574232846
6942 6942 a, c dbSNP:541556786
6956 6956 a, g dbSNP:183379085
6963 6963 a, t dbSNP:573365424
7028 7028 -, t dbSNP:765132380
7084 7084 a, g dbSNP:749236455
7085 7085 -, t dbSNP:542785965
7132 7132 a, t dbSNP:10852019
7165 7165 c, t dbSNP:565597750
7166 7166 c, t dbSNP:151078068
7167 7167 a, g dbSNP:188784206
7187 7187 c, t dbSNP:562870172
7195 7195 a, g dbSNP:530058944
7237 7237 c, g dbSNP:141044168

Target ORF information:

RefSeq Version XM_005254202
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254202

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Accession Version XM_011521288.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011519590.1
CDS81..1352
Misc Feature(1)81..392(+)
Misc Feature(2)81..290(+)
Misc Feature(3)1011..1301(+)
Translation
Position Chain Variation Link
6 6 a, g dbSNP:751901647
9 9 c, t dbSNP:114106593
22 22 a, g dbSNP:748512713
41 41 -, t dbSNP:767219677
42 42 c, t dbSNP:79328043
59 59 c, t dbSNP:764234046
60 60 a, g dbSNP:753924528
64 64 a, g, t dbSNP:757726772
69 69 c, g dbSNP:750777752
70 70 a, g dbSNP:758855467
75 75 c, g dbSNP:780614363
77 77 c, g dbSNP:367745143
86 86 c, t dbSNP:755788325
87 87 c, t dbSNP:121434313
136 136 a, c, g dbSNP:781390048
140 140 c, t dbSNP:748759533
141 141 a, g dbSNP:147204964
148 148 a, t dbSNP:121434318
151 151 g, t dbSNP:778847063
158 158 c, t dbSNP:745859168
160 160 c, t dbSNP:771825294
165 165 c, t dbSNP:148646547
180 180 c, t dbSNP:775334473
185 185 g, t dbSNP:760939387
189 189 a, t dbSNP:768775931
194 194 c, t dbSNP:397517871
195 195 a, g dbSNP:761830220
198 198 c, t dbSNP:765788978
199 199 a, g, t dbSNP:750686148
207 207 c, t dbSNP:121434315
208 208 a, g dbSNP:372205720
213 213 a, c dbSNP:751923342
228 228 a, g dbSNP:369150309
245 245 a, t dbSNP:763749955
246 246 a, t dbSNP:727504170
287 287 c, g, t dbSNP:773943895
289 289 a, g dbSNP:756975029
296 296 a, g dbSNP:778384985
297 297 a, g dbSNP:749950643
298 298 c, t dbSNP:761473300
307 307 a, g dbSNP:556636985
308 308 a, g dbSNP:7182445
322 322 c, t dbSNP:754706111
323 323 a, g dbSNP:372791883
328 328 a, g dbSNP:748376033
338 338 a, t dbSNP:770197803
365 365 c, t dbSNP:773363523
366 366 c, t dbSNP:121434312
367 367 a, g dbSNP:749378713
370 370 a, g dbSNP:373381933
375 375 a, g dbSNP:774740245
388 388 c, t dbSNP:760132893
390 390 c, t dbSNP:767771754
401 401 c, t dbSNP:761058615
402 402 a, g dbSNP:764823722
405 405 c, t dbSNP:149883080
413 413 c, t dbSNP:772830013
417 417 a, g dbSNP:755991015
418 418 c, g dbSNP:762633609
422 422 a, g dbSNP:778868909
425 425 g, t dbSNP:765800186
430 430 a, g dbSNP:751138842
434 434 c, t dbSNP:12148911
441 441 g, t dbSNP:763971890
443 443 a, g dbSNP:753701682
455 455 c, t dbSNP:757357920
463 463 a, g dbSNP:373384814
468 468 c, g dbSNP:750421037
480 480 c, g, t dbSNP:758483715
482 482 c, t dbSNP:376944837
487 487 a, t dbSNP:747508368
493 493 a, c dbSNP:769206547
494 494 a, t dbSNP:781477854
512 512 g, t dbSNP:748550020
515 515 c, g dbSNP:770446191
520 520 a, t dbSNP:774070689
524 524 a, c dbSNP:759158378
527 527 c, t dbSNP:771673657
528 528 c, t dbSNP:376527959
536 536 a, g dbSNP:532147114
541 541 -, ctt dbSNP:763365429
547 547 c, t dbSNP:553832516
552 552 a, g dbSNP:143121426
577 577 a, g dbSNP:764135694
578 578 c, t dbSNP:201335683
580 580 c, t dbSNP:370735097
581 581 a, g dbSNP:202092705
582 582 a, c dbSNP:200142785
584 584 a, c, t dbSNP:764990934
587 587 c, t dbSNP:758617124
595 595 a, g dbSNP:766431294
604 604 c, t dbSNP:147474792
611 611 c, t dbSNP:756643495
616 616 c, t dbSNP:778144652
624 624 a, g dbSNP:139897054
627 627 a, g dbSNP:758087535
631 631 a, g dbSNP:779879921
635 635 c, g dbSNP:746708059
636 636 a, c dbSNP:368660900
641 641 c, t dbSNP:776073446
650 650 c, t dbSNP:149991304
651 651 a, g dbSNP:769630742
654 654 c, t dbSNP:121434316
657 657 a, c, t dbSNP:376699107
658 658 a, g dbSNP:371274407
660 660 c, g, t dbSNP:121434314
662 662 a, c dbSNP:144227155
680 680 a, g dbSNP:766539664
686 686 a, g dbSNP:774530752
692 692 c, g, t dbSNP:144764225
702 702 g, t dbSNP:201837340
704 704 a, c dbSNP:376226762
713 713 a, g dbSNP:764287445
719 719 c, g dbSNP:138553244
729 729 a, g dbSNP:762478369
748 748 a, g dbSNP:766152186
782 782 a, g dbSNP:765684707
787 787 a, g dbSNP:750832911
801 801 a, t dbSNP:121434317
812 812 c, t dbSNP:116667267
838 838 a, g dbSNP:111363743
840 840 g, t dbSNP:780737673
852 852 a, g dbSNP:752201824
858 858 c, t dbSNP:755557783
871 871 c, t dbSNP:759197561
881 881 a, c dbSNP:777379558
883 883 -, aatcagact dbSNP:778423017
883 883 a, c dbSNP:372665535
892 892 a, g dbSNP:201933663
896 896 a, g dbSNP:535687467
903 903 c, t dbSNP:554327738
904 904 a, g dbSNP:200974459
905 905 c, t dbSNP:775556490
909 909 c, g dbSNP:761001792
916 916 c, g dbSNP:540124243
923 923 a, g dbSNP:776909752
937 937 a, g dbSNP:764868020
939 939 a, g, t dbSNP:765841849
943 943 c, t dbSNP:114636635
945 945 a, g dbSNP:752191960
948 948 c, t dbSNP:375700452
955 955 c, t dbSNP:766814966
956 956 a, g, t dbSNP:140644874
961 961 c, t dbSNP:115440602
971 971 a, g dbSNP:753357510
980 980 -, gaagtc dbSNP:749692794
980 980 a, g dbSNP:369711772
994 994 c, g dbSNP:750890621
1002 1002 a, c, g dbSNP:756634323
1011 1011 c, t dbSNP:758102077
1012 1012 a, g dbSNP:779966392
1017 1017 c, t dbSNP:765603808
1018 1018 a, g dbSNP:146702985
1022 1022 c, t dbSNP:768937237
1023 1023 a, g dbSNP:373477920
1048 1048 a, g dbSNP:376679136
1052 1052 a, g dbSNP:770375243
1054 1054 a, g dbSNP:773833198
1061 1061 c, g, t dbSNP:3751526
1062 1062 a, c, t dbSNP:774835982
1090 1090 c, g dbSNP:760186912
1093 1093 a, t dbSNP:763738243
1095 1095 a, g dbSNP:374685699
1097 1097 a, g dbSNP:749654043
1103 1103 c, t dbSNP:756739740
1104 1104 a, g dbSNP:764768156
1106 1106 a, g dbSNP:140225135
1113 1113 a, g dbSNP:372623722
1122 1122 a, t dbSNP:779660163
1125 1125 c, t dbSNP:397517870
1145 1145 g, t dbSNP:746827242
1164 1164 c, t dbSNP:755208632
1167 1167 -, g dbSNP:771343745
1187 1187 c, t dbSNP:781585106
1193 1193 a, g dbSNP:755223172
1203 1203 a, g dbSNP:769959530
1205 1205 c, t dbSNP:773409045
1208 1208 c, t dbSNP:749745659
1209 1209 a, g dbSNP:771480941
1211 1211 -, c dbSNP:774533789
1219 1219 g, t dbSNP:779058019
1221 1221 c, t dbSNP:759969513
1225 1225 a, g dbSNP:772476060
1227 1227 g, t dbSNP:776235336
1233 1233 a, g dbSNP:761520561
1234 1234 c, t dbSNP:748390368
1239 1239 g, t dbSNP:150307819
1242 1242 a, g, t dbSNP:762735151
1243 1243 c, t dbSNP:751260227
1256 1256 c, t dbSNP:754794684
1277 1277 c, t dbSNP:202087411
1278 1278 a, g dbSNP:752958999
1290 1290 a, g dbSNP:368307475
1300 1300 a, g dbSNP:369492789
1306 1306 c, g dbSNP:749389617
1312 1312 c, t dbSNP:200871227
1315 1315 a, g dbSNP:779454951
1318 1318 g, t dbSNP:746414913
1324 1324 a, g dbSNP:772421738
1330 1330 g, t dbSNP:573603750
1336 1336 -, at dbSNP:759838497
1341 1341 a, g dbSNP:761401264
1354 1354 a, c dbSNP:769445247
1385 1385 -, ata dbSNP:772254530
1399 1399 c, t dbSNP:772560499
1400 1400 a, g dbSNP:373694127
1416 1416 c, t dbSNP:544137820
1418 1418 a, g dbSNP:533114849
1433 1433 c, t dbSNP:773453342
1457 1457 a, g dbSNP:545295358
1495 1495 a, c dbSNP:770816015
1501 1501 a, g dbSNP:776485386
1520 1520 c, t dbSNP:192826011
1525 1525 c, t dbSNP:765093939
1535 1535 a, g dbSNP:530541798
1542 1542 a, g dbSNP:377091058
1557 1557 a, g dbSNP:567404158
1578 1578 a, g dbSNP:377201217
1600 1600 c, t dbSNP:16966842
1602 1602 a, g dbSNP:547722924
1608 1608 c, t dbSNP:145373657
1617 1617 a, c dbSNP:539607759
1673 1673 a, g dbSNP:137920898
1710 1710 c, g dbSNP:569990101
1711 1711 a, g dbSNP:537079236
1712 1712 a, g dbSNP:751117768
1734 1734 c, g dbSNP:13329392
1743 1743 g, t dbSNP:555317480
1779 1779 a, t dbSNP:185290497
1797 1797 a, g dbSNP:577788958
1807 1807 c, g dbSNP:545070531
1882 1882 a, g dbSNP:756612869
1887 1887 a, g dbSNP:370660664
1916 1916 a, t dbSNP:373626511
1930 1930 -, at dbSNP:147547509
1931 1931 -, atat dbSNP:759359048
1933 1933 c, t dbSNP:541503324
1942 1942 a, c dbSNP:553150319
1944 1944 a, c dbSNP:754213247
1946 1946 a, c dbSNP:577769507
1985 1985 a, g dbSNP:118146919
2014 2014 a, g dbSNP:374443282
2017 2017 c, t dbSNP:755236404
2071 2071 a, g dbSNP:563589753
2088 2088 a, t dbSNP:188754645
2108 2108 c, t dbSNP:530810790
2110 2110 c, t dbSNP:560005063
2135 2135 c, t dbSNP:770577944
2188 2188 a, g dbSNP:192636064
2221 2221 -, att dbSNP:541409661
2245 2245 c, t dbSNP:542673653
2252 2252 c, t dbSNP:560864932
2295 2295 g, t dbSNP:183715838
2392 2392 a, g dbSNP:115568221
2411 2411 -, a dbSNP:34079057
2458 2458 c, t dbSNP:571210831
2466 2466 g, t dbSNP:141742028
2544 2544 c, t dbSNP:28536719
2546 2546 c, t dbSNP:28576519
2577 2577 c, t dbSNP:551528743
2629 2629 c, g dbSNP:779337443
2650 2650 a, g dbSNP:376795432
2660 2660 a, g dbSNP:368946709
2689 2689 a, c dbSNP:570038934
2723 2723 c, t dbSNP:373111945
2775 2775 a, g dbSNP:748337294
2804 2804 a, c dbSNP:751531938
2842 2842 g, t dbSNP:555380695
2844 2844 g, t dbSNP:199891616
2866 2866 g, t dbSNP:376829790
2868 2868 a, g dbSNP:757780122
3009 3009 -, ctat dbSNP:146972863
3011 3011 -, atct dbSNP:57589132
3011 3011 a, g dbSNP:113071870
3072 3072 c, t dbSNP:567659062
3076 3076 a, c dbSNP:1554527
3085 3085 a, g dbSNP:777972348
3095 3095 c, t dbSNP:747157552
3099 3099 a, c dbSNP:561525888
3113 3113 c, t dbSNP:553453653
3118 3118 -, aag dbSNP:546611820
3161 3161 a, g dbSNP:578008401
3164 3164 a, g dbSNP:770892084
3189 3189 a, g dbSNP:778489832
3247 3247 c, t dbSNP:538871697
3249 3249 c, t dbSNP:776375597
3370 3370 g, t dbSNP:188392935
3395 3395 a, g dbSNP:180946373
3432 3432 c, g dbSNP:759262779
3565 3565 c, t dbSNP:542935372
3599 3599 a, g dbSNP:118057877
3674 3674 a, g dbSNP:747836383
3729 3729 a, g dbSNP:572802254
3764 3764 -, t dbSNP:532033889
3772 3772 c, t dbSNP:184024152
3837 3837 a, g dbSNP:565009702
3838 3838 a, t dbSNP:374589452
3854 3854 -, tgagtaaa dbSNP:770258605
3854 3854 g, t dbSNP:532084025
3915 3915 -, at dbSNP:373432788
3922 3922 c, t dbSNP:111997668
3950 3950 a, c dbSNP:189861748
3952 3952 c, t dbSNP:775287695
3957 3957 c, t dbSNP:530967462
3963 3963 c, t dbSNP:181716461
3964 3964 a, g dbSNP:567834681
3974 3974 a, g dbSNP:138645237
3987 3987 c, g dbSNP:546662922
4038 4038 a, t dbSNP:571741901
4061 4061 c, t dbSNP:539133361
4062 4062 g, t dbSNP:557234725
4075 4075 c, t dbSNP:762569457
4080 4080 -, t dbSNP:773860286
4085 4085 a, t dbSNP:763774696
4086 4086 c, g dbSNP:773847026
4094 4094 g, t dbSNP:149336440
4104 4104 -, g dbSNP:746468942
4138 4138 c, g dbSNP:147428585
4176 4176 c, g dbSNP:761286407
4183 4183 a, g dbSNP:35490364
4200 4200 c, t dbSNP:754227753
4205 4205 a, g dbSNP:187134665
4237 4237 a, g dbSNP:79309779
4260 4260 c, t dbSNP:765645509
4296 4296 a, t dbSNP:759460441
4298 4298 a, c dbSNP:564720434
4352 4352 g, t dbSNP:753155345
4381 4381 -, gctgaaa dbSNP:553420659
4409 4409 a, g dbSNP:72725362
4419 4419 -, tt dbSNP:771536852
4430 4430 g, t dbSNP:543909086
4463 4463 c, t dbSNP:111766079
4483 4483 c, t dbSNP:373027564
4490 4490 a, g dbSNP:531030936
4516 4516 a, g dbSNP:191914374
4544 4544 a, g dbSNP:566065329
4606 4606 a, g dbSNP:561659585
4613 4613 c, t dbSNP:751824076
4665 4665 a, c, t dbSNP:536604218
4667 4667 a, g dbSNP:757217761
4675 4675 c, t dbSNP:547019744
4683 4683 a, g dbSNP:781366830
4692 4692 a, g dbSNP:571542327
4746 4746 c, t dbSNP:548164209
4747 4747 a, g dbSNP:140749542
4767 4767 a, c dbSNP:551099276
4811 4811 a, g dbSNP:764169369
4836 4836 -, tt dbSNP:774937535
4873 4873 a, g dbSNP:11634702
4904 4904 a, g dbSNP:376186397
4912 4912 a, g dbSNP:200294834
4913 4913 a, g dbSNP:181980647
4924 4924 a, g dbSNP:113753698
4933 4933 g, t dbSNP:779791635
4935 4935 a, c, g dbSNP:566802137
4943 4943 c, t dbSNP:147489619
4949 4949 -, ta dbSNP:574998297
4963 4963 a, g dbSNP:558773794
4975 4975 g, t dbSNP:148525432
4979 4979 -, a dbSNP:546844553
4997 4997 -, g dbSNP:763956861
4999 4999 g, t dbSNP:186366365
5009 5009 c, t dbSNP:761527047
5050 5050 g, t dbSNP:76527876
5053 5053 a, g dbSNP:574179000
5078 5078 a, g dbSNP:543233753
5097 5097 a, g dbSNP:142928983
5140 5140 c, g dbSNP:577731830
5142 5142 c, t dbSNP:528822175
5157 5157 a, t dbSNP:759931845
5182 5182 a, g dbSNP:8039150
5184 5184 a, g dbSNP:565528602
5193 5193 g, t dbSNP:532564269
5244 5244 g, t dbSNP:112221665
5298 5298 a, g dbSNP:8039337
5348 5348 a, g dbSNP:72725364
5394 5394 a, c dbSNP:189589145
5429 5429 a, t dbSNP:572054998
5453 5453 a, g dbSNP:182237934
5454 5454 c, t dbSNP:577333581
5488 5488 -, att dbSNP:369376164
5519 5519 a, g dbSNP:758092431
5546 5546 a, t dbSNP:777252680
5558 5558 a, g dbSNP:746558881
5583 5583 a, g dbSNP:533950389
5589 5589 a, g dbSNP:558595140
5593 5593 c, g dbSNP:570386455
5616 5616 -, a dbSNP:542372343
5634 5634 -, ggtat dbSNP:767679657
5656 5656 a, g dbSNP:537477031
5700 5700 c, t dbSNP:375141692
5724 5724 a, g dbSNP:187788660
5728 5728 g, t dbSNP:781111622
5786 5786 c, t dbSNP:750554498
5788 5788 a, g dbSNP:191601293
5803 5803 -, at dbSNP:761502926
5823 5823 a, c dbSNP:574232846
5824 5824 a, c dbSNP:541556786
5838 5838 a, g dbSNP:183379085
5845 5845 a, t dbSNP:573365424
5910 5910 -, t dbSNP:765132380
5966 5966 a, g dbSNP:749236455
5967 5967 -, t dbSNP:542785965
6014 6014 a, t dbSNP:10852019
6047 6047 c, t dbSNP:565597750
6048 6048 c, t dbSNP:151078068
6049 6049 a, g dbSNP:188784206
6069 6069 c, t dbSNP:562870172
6077 6077 a, g dbSNP:530058944
6119 6119 c, g dbSNP:141044168

Target ORF information:

RefSeq Version XM_011521288
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521288

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Accession Version XM_011521289.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011519591.1
CDS96..1367
Misc Feature(1)96..407(+)
Misc Feature(2)96..305(+)
Misc Feature(3)1026..1316(+)
Translation
Position Chain Variation Link
25 25 g, t dbSNP:753212180
35 35 a, c dbSNP:763286096
39 39 a, g dbSNP:764585879
74 74 c, t dbSNP:764234046
75 75 a, g dbSNP:753924528
79 79 a, g, t dbSNP:757726772
84 84 c, g dbSNP:750777752
85 85 a, g dbSNP:758855467
90 90 c, g dbSNP:780614363
92 92 c, g dbSNP:367745143
101 101 c, t dbSNP:755788325
102 102 c, t dbSNP:121434313
151 151 a, c, g dbSNP:781390048
155 155 c, t dbSNP:748759533
156 156 a, g dbSNP:147204964
163 163 a, t dbSNP:121434318
166 166 g, t dbSNP:778847063
173 173 c, t dbSNP:745859168
175 175 c, t dbSNP:771825294
180 180 c, t dbSNP:148646547
195 195 c, t dbSNP:775334473
200 200 g, t dbSNP:760939387
204 204 a, t dbSNP:768775931
209 209 c, t dbSNP:397517871
210 210 a, g dbSNP:761830220
213 213 c, t dbSNP:765788978
214 214 a, g, t dbSNP:750686148
222 222 c, t dbSNP:121434315
223 223 a, g dbSNP:372205720
228 228 a, c dbSNP:751923342
243 243 a, g dbSNP:369150309
260 260 a, t dbSNP:763749955
261 261 a, t dbSNP:727504170
302 302 c, g, t dbSNP:773943895
304 304 a, g dbSNP:756975029
311 311 a, g dbSNP:778384985
312 312 a, g dbSNP:749950643
313 313 c, t dbSNP:761473300
322 322 a, g dbSNP:556636985
323 323 a, g dbSNP:7182445
337 337 c, t dbSNP:754706111
338 338 a, g dbSNP:372791883
343 343 a, g dbSNP:748376033
353 353 a, t dbSNP:770197803
380 380 c, t dbSNP:773363523
381 381 c, t dbSNP:121434312
382 382 a, g dbSNP:749378713
385 385 a, g dbSNP:373381933
390 390 a, g dbSNP:774740245
403 403 c, t dbSNP:760132893
405 405 c, t dbSNP:767771754
416 416 c, t dbSNP:761058615
417 417 a, g dbSNP:764823722
420 420 c, t dbSNP:149883080
428 428 c, t dbSNP:772830013
432 432 a, g dbSNP:755991015
433 433 c, g dbSNP:762633609
437 437 a, g dbSNP:778868909
440 440 g, t dbSNP:765800186
445 445 a, g dbSNP:751138842
449 449 c, t dbSNP:12148911
456 456 g, t dbSNP:763971890
458 458 a, g dbSNP:753701682
470 470 c, t dbSNP:757357920
478 478 a, g dbSNP:373384814
483 483 c, g dbSNP:750421037
495 495 c, g, t dbSNP:758483715
497 497 c, t dbSNP:376944837
502 502 a, t dbSNP:747508368
508 508 a, c dbSNP:769206547
509 509 a, t dbSNP:781477854
527 527 g, t dbSNP:748550020
530 530 c, g dbSNP:770446191
535 535 a, t dbSNP:774070689
539 539 a, c dbSNP:759158378
542 542 c, t dbSNP:771673657
543 543 c, t dbSNP:376527959
551 551 a, g dbSNP:532147114
556 556 -, ctt dbSNP:763365429
562 562 c, t dbSNP:553832516
567 567 a, g dbSNP:143121426
592 592 a, g dbSNP:764135694
593 593 c, t dbSNP:201335683
595 595 c, t dbSNP:370735097
596 596 a, g dbSNP:202092705
597 597 a, c dbSNP:200142785
599 599 a, c, t dbSNP:764990934
602 602 c, t dbSNP:758617124
610 610 a, g dbSNP:766431294
619 619 c, t dbSNP:147474792
626 626 c, t dbSNP:756643495
631 631 c, t dbSNP:778144652
639 639 a, g dbSNP:139897054
642 642 a, g dbSNP:758087535
646 646 a, g dbSNP:779879921
650 650 c, g dbSNP:746708059
651 651 a, c dbSNP:368660900
656 656 c, t dbSNP:776073446
665 665 c, t dbSNP:149991304
666 666 a, g dbSNP:769630742
669 669 c, t dbSNP:121434316
672 672 a, c, t dbSNP:376699107
673 673 a, g dbSNP:371274407
675 675 c, g, t dbSNP:121434314
677 677 a, c dbSNP:144227155
695 695 a, g dbSNP:766539664
701 701 a, g dbSNP:774530752
707 707 c, g, t dbSNP:144764225
717 717 g, t dbSNP:201837340
719 719 a, c dbSNP:376226762
728 728 a, g dbSNP:764287445
734 734 c, g dbSNP:138553244
744 744 a, g dbSNP:762478369
763 763 a, g dbSNP:766152186
797 797 a, g dbSNP:765684707
802 802 a, g dbSNP:750832911
816 816 a, t dbSNP:121434317
827 827 c, t dbSNP:116667267
853 853 a, g dbSNP:111363743
855 855 g, t dbSNP:780737673
867 867 a, g dbSNP:752201824
873 873 c, t dbSNP:755557783
886 886 c, t dbSNP:759197561
896 896 a, c dbSNP:777379558
898 898 -, aatcagact dbSNP:778423017
898 898 a, c dbSNP:372665535
907 907 a, g dbSNP:201933663
911 911 a, g dbSNP:535687467
918 918 c, t dbSNP:554327738
919 919 a, g dbSNP:200974459
920 920 c, t dbSNP:775556490
924 924 c, g dbSNP:761001792
931 931 c, g dbSNP:540124243
938 938 a, g dbSNP:776909752
952 952 a, g dbSNP:764868020
954 954 a, g, t dbSNP:765841849
958 958 c, t dbSNP:114636635
960 960 a, g dbSNP:752191960
963 963 c, t dbSNP:375700452
970 970 c, t dbSNP:766814966
971 971 a, g, t dbSNP:140644874
976 976 c, t dbSNP:115440602
986 986 a, g dbSNP:753357510
995 995 -, gaagtc dbSNP:749692794
995 995 a, g dbSNP:369711772
1009 1009 c, g dbSNP:750890621
1017 1017 a, c, g dbSNP:756634323
1026 1026 c, t dbSNP:758102077
1027 1027 a, g dbSNP:779966392
1032 1032 c, t dbSNP:765603808
1033 1033 a, g dbSNP:146702985
1037 1037 c, t dbSNP:768937237
1038 1038 a, g dbSNP:373477920
1063 1063 a, g dbSNP:376679136
1067 1067 a, g dbSNP:770375243
1069 1069 a, g dbSNP:773833198
1076 1076 c, g, t dbSNP:3751526
1077 1077 a, c, t dbSNP:774835982
1105 1105 c, g dbSNP:760186912
1108 1108 a, t dbSNP:763738243
1110 1110 a, g dbSNP:374685699
1112 1112 a, g dbSNP:749654043
1118 1118 c, t dbSNP:756739740
1119 1119 a, g dbSNP:764768156
1121 1121 a, g dbSNP:140225135
1128 1128 a, g dbSNP:372623722
1137 1137 a, t dbSNP:779660163
1140 1140 c, t dbSNP:397517870
1160 1160 g, t dbSNP:746827242
1179 1179 c, t dbSNP:755208632
1182 1182 -, g dbSNP:771343745
1202 1202 c, t dbSNP:781585106
1208 1208 a, g dbSNP:755223172
1218 1218 a, g dbSNP:769959530
1220 1220 c, t dbSNP:773409045
1223 1223 c, t dbSNP:749745659
1224 1224 a, g dbSNP:771480941
1226 1226 -, c dbSNP:774533789
1234 1234 g, t dbSNP:779058019
1236 1236 c, t dbSNP:759969513
1240 1240 a, g dbSNP:772476060
1242 1242 g, t dbSNP:776235336
1248 1248 a, g dbSNP:761520561
1249 1249 c, t dbSNP:748390368
1254 1254 g, t dbSNP:150307819
1257 1257 a, g, t dbSNP:762735151
1258 1258 c, t dbSNP:751260227
1271 1271 c, t dbSNP:754794684
1292 1292 c, t dbSNP:202087411
1293 1293 a, g dbSNP:752958999
1305 1305 a, g dbSNP:368307475
1315 1315 a, g dbSNP:369492789
1321 1321 c, g dbSNP:749389617
1327 1327 c, t dbSNP:200871227
1330 1330 a, g dbSNP:779454951
1333 1333 g, t dbSNP:746414913
1339 1339 a, g dbSNP:772421738
1345 1345 g, t dbSNP:573603750
1351 1351 -, at dbSNP:759838497
1356 1356 a, g dbSNP:761401264
1369 1369 a, c dbSNP:769445247
1400 1400 -, ata dbSNP:772254530
1414 1414 c, t dbSNP:772560499
1415 1415 a, g dbSNP:373694127
1431 1431 c, t dbSNP:544137820
1433 1433 a, g dbSNP:533114849
1448 1448 c, t dbSNP:773453342
1472 1472 a, g dbSNP:545295358
1510 1510 a, c dbSNP:770816015
1516 1516 a, g dbSNP:776485386
1535 1535 c, t dbSNP:192826011
1540 1540 c, t dbSNP:765093939
1550 1550 a, g dbSNP:530541798
1557 1557 a, g dbSNP:377091058
1572 1572 a, g dbSNP:567404158
1593 1593 a, g dbSNP:377201217
1615 1615 c, t dbSNP:16966842
1617 1617 a, g dbSNP:547722924
1623 1623 c, t dbSNP:145373657
1632 1632 a, c dbSNP:539607759
1688 1688 a, g dbSNP:137920898
1725 1725 c, g dbSNP:569990101
1726 1726 a, g dbSNP:537079236
1727 1727 a, g dbSNP:751117768
1749 1749 c, g dbSNP:13329392
1758 1758 g, t dbSNP:555317480
1794 1794 a, t dbSNP:185290497
1812 1812 a, g dbSNP:577788958
1822 1822 c, g dbSNP:545070531
1897 1897 a, g dbSNP:756612869
1902 1902 a, g dbSNP:370660664
1931 1931 a, t dbSNP:373626511
1945 1945 -, at dbSNP:147547509
1946 1946 -, atat dbSNP:759359048
1948 1948 c, t dbSNP:541503324
1957 1957 a, c dbSNP:553150319
1959 1959 a, c dbSNP:754213247
1961 1961 a, c dbSNP:577769507
2000 2000 a, g dbSNP:118146919
2029 2029 a, g dbSNP:374443282
2032 2032 c, t dbSNP:755236404
2086 2086 a, g dbSNP:563589753
2103 2103 a, t dbSNP:188754645
2123 2123 c, t dbSNP:530810790
2125 2125 c, t dbSNP:560005063
2150 2150 c, t dbSNP:770577944
2203 2203 a, g dbSNP:192636064
2236 2236 -, att dbSNP:541409661
2260 2260 c, t dbSNP:542673653
2267 2267 c, t dbSNP:560864932
2310 2310 g, t dbSNP:183715838
2407 2407 a, g dbSNP:115568221
2426 2426 -, a dbSNP:34079057
2473 2473 c, t dbSNP:571210831
2481 2481 g, t dbSNP:141742028
2559 2559 c, t dbSNP:28536719
2561 2561 c, t dbSNP:28576519
2592 2592 c, t dbSNP:551528743
2644 2644 c, g dbSNP:779337443
2665 2665 a, g dbSNP:376795432
2675 2675 a, g dbSNP:368946709
2704 2704 a, c dbSNP:570038934
2738 2738 c, t dbSNP:373111945
2790 2790 a, g dbSNP:748337294
2819 2819 a, c dbSNP:751531938
2857 2857 g, t dbSNP:555380695
2859 2859 g, t dbSNP:199891616
2881 2881 g, t dbSNP:376829790
2883 2883 a, g dbSNP:757780122
3024 3024 -, ctat dbSNP:146972863
3026 3026 -, atct dbSNP:57589132
3026 3026 a, g dbSNP:113071870
3087 3087 c, t dbSNP:567659062
3091 3091 a, c dbSNP:1554527
3100 3100 a, g dbSNP:777972348
3110 3110 c, t dbSNP:747157552
3114 3114 a, c dbSNP:561525888
3128 3128 c, t dbSNP:553453653
3133 3133 -, aag dbSNP:546611820
3176 3176 a, g dbSNP:578008401
3179 3179 a, g dbSNP:770892084
3204 3204 a, g dbSNP:778489832
3262 3262 c, t dbSNP:538871697
3264 3264 c, t dbSNP:776375597
3385 3385 g, t dbSNP:188392935
3410 3410 a, g dbSNP:180946373
3447 3447 c, g dbSNP:759262779
3580 3580 c, t dbSNP:542935372
3614 3614 a, g dbSNP:118057877
3689 3689 a, g dbSNP:747836383
3744 3744 a, g dbSNP:572802254
3779 3779 -, t dbSNP:532033889
3787 3787 c, t dbSNP:184024152
3852 3852 a, g dbSNP:565009702
3853 3853 a, t dbSNP:374589452
3869 3869 -, tgagtaaa dbSNP:770258605
3869 3869 g, t dbSNP:532084025
3930 3930 -, at dbSNP:373432788
3937 3937 c, t dbSNP:111997668
3965 3965 a, c dbSNP:189861748
3967 3967 c, t dbSNP:775287695
3972 3972 c, t dbSNP:530967462
3978 3978 c, t dbSNP:181716461
3979 3979 a, g dbSNP:567834681
3989 3989 a, g dbSNP:138645237
4002 4002 c, g dbSNP:546662922
4053 4053 a, t dbSNP:571741901
4076 4076 c, t dbSNP:539133361
4077 4077 g, t dbSNP:557234725
4090 4090 c, t dbSNP:762569457
4095 4095 -, t dbSNP:773860286
4100 4100 a, t dbSNP:763774696
4101 4101 c, g dbSNP:773847026
4109 4109 g, t dbSNP:149336440
4119 4119 -, g dbSNP:746468942
4153 4153 c, g dbSNP:147428585
4191 4191 c, g dbSNP:761286407
4198 4198 a, g dbSNP:35490364
4215 4215 c, t dbSNP:754227753
4220 4220 a, g dbSNP:187134665
4252 4252 a, g dbSNP:79309779
4275 4275 c, t dbSNP:765645509
4311 4311 a, t dbSNP:759460441
4313 4313 a, c dbSNP:564720434
4367 4367 g, t dbSNP:753155345
4396 4396 -, gctgaaa dbSNP:553420659
4424 4424 a, g dbSNP:72725362
4434 4434 -, tt dbSNP:771536852
4445 4445 g, t dbSNP:543909086
4478 4478 c, t dbSNP:111766079
4498 4498 c, t dbSNP:373027564
4505 4505 a, g dbSNP:531030936
4531 4531 a, g dbSNP:191914374
4559 4559 a, g dbSNP:566065329
4621 4621 a, g dbSNP:561659585
4628 4628 c, t dbSNP:751824076
4680 4680 a, c, t dbSNP:536604218
4682 4682 a, g dbSNP:757217761
4690 4690 c, t dbSNP:547019744
4698 4698 a, g dbSNP:781366830
4707 4707 a, g dbSNP:571542327
4761 4761 c, t dbSNP:548164209
4762 4762 a, g dbSNP:140749542
4782 4782 a, c dbSNP:551099276
4826 4826 a, g dbSNP:764169369
4851 4851 -, tt dbSNP:774937535
4888 4888 a, g dbSNP:11634702
4919 4919 a, g dbSNP:376186397
4927 4927 a, g dbSNP:200294834
4928 4928 a, g dbSNP:181980647
4939 4939 a, g dbSNP:113753698
4948 4948 g, t dbSNP:779791635
4950 4950 a, c, g dbSNP:566802137
4958 4958 c, t dbSNP:147489619
4964 4964 -, ta dbSNP:574998297
4978 4978 a, g dbSNP:558773794
4990 4990 g, t dbSNP:148525432
4994 4994 -, a dbSNP:546844553
5012 5012 -, g dbSNP:763956861
5014 5014 g, t dbSNP:186366365
5024 5024 c, t dbSNP:761527047
5065 5065 g, t dbSNP:76527876
5068 5068 a, g dbSNP:574179000
5093 5093 a, g dbSNP:543233753
5112 5112 a, g dbSNP:142928983
5155 5155 c, g dbSNP:577731830
5157 5157 c, t dbSNP:528822175
5172 5172 a, t dbSNP:759931845
5197 5197 a, g dbSNP:8039150
5199 5199 a, g dbSNP:565528602
5208 5208 g, t dbSNP:532564269
5259 5259 g, t dbSNP:112221665
5313 5313 a, g dbSNP:8039337
5363 5363 a, g dbSNP:72725364
5409 5409 a, c dbSNP:189589145
5444 5444 a, t dbSNP:572054998
5468 5468 a, g dbSNP:182237934
5469 5469 c, t dbSNP:577333581
5503 5503 -, att dbSNP:369376164
5534 5534 a, g dbSNP:758092431
5561 5561 a, t dbSNP:777252680
5573 5573 a, g dbSNP:746558881
5598 5598 a, g dbSNP:533950389
5604 5604 a, g dbSNP:558595140
5608 5608 c, g dbSNP:570386455
5631 5631 -, a dbSNP:542372343
5649 5649 -, ggtat dbSNP:767679657
5671 5671 a, g dbSNP:537477031
5715 5715 c, t dbSNP:375141692
5739 5739 a, g dbSNP:187788660
5743 5743 g, t dbSNP:781111622
5801 5801 c, t dbSNP:750554498
5803 5803 a, g dbSNP:191601293
5818 5818 -, at dbSNP:761502926
5838 5838 a, c dbSNP:574232846
5839 5839 a, c dbSNP:541556786
5853 5853 a, g dbSNP:183379085
5860 5860 a, t dbSNP:573365424
5925 5925 -, t dbSNP:765132380
5981 5981 a, g dbSNP:749236455
5982 5982 -, t dbSNP:542785965
6029 6029 a, t dbSNP:10852019
6062 6062 c, t dbSNP:565597750
6063 6063 c, t dbSNP:151078068
6064 6064 a, g dbSNP:188784206
6084 6084 c, t dbSNP:562870172
6092 6092 a, g dbSNP:530058944
6134 6134 c, g dbSNP:141044168

Target ORF information:

RefSeq Version XM_011521289
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48144
Accession Version XM_005254203.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578826670. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTTAAAA AAGACCTCAT TTATAATAAG GTCACTCCAA CATTTCACCA CTGGAAGATT 
GATGACAAGA AGTTTGGTCT TACGTTTCAA AGTCCTGCTG ATGCTAGGGC TTTTGATAGA
GGTATCCGAA GAGCTATAGA GGATATTTCT CAAGGATGCC CCGAATCAAA AAATGAAGCT
GAAGGGGCAG ATGACTTACA AGCAAATGAA GAGGATTCTT CCAGTTCTCT AGTGAAGGAT
CACCTTTTTC AGCAAGAGAC AGTTGTTACC AGTGAGCCTT ATAGAAGCTC AAATATAAGA
CCTTCTCCCT TTGAAGATCT GAATGCCAGA AGAGTCTACA TGCAAAGCCA AGCCAATCAG
ATAACATTTG GTCAGCCAGG CTTGGACATT CAGAGCAGAA GTATGGAATA CGTACAGCGG
CAAATATCCA AGGAATGTGG AAGCCTAAAG TCCCAAAATA GGGTCCCTTT GAAATCAATC
AGACATGTCA GCTTTCAAGA TGAGGATGAG ATTGTCAGAA TAAACCCTCG AGATATCTTA
ATACGTCGCT ATGCAGACTA CAGACATCCT GACATGTGGA AAAATGACTT GGAAAGAGAT
GATGCTGATT CCAGTATTCA GTTTTCTAAA CCAGACAGTA AAAAATCAGA CTATCTGTAC
TCTTGTGGGG ATGAGACTAA GTTAAGTTCA CCCAAAGACT CTGTGGTATT TAAGACGCAG
CCTTCCTCAT TAAAAATTAA GAAGTCAAAA CGAAGAAAAG AGGATGGTGA ACGTTCTCGC
TGCGTATACT GCCAGGAAAG GTTTAATCAT GAAGAAAATG TTAGGGGAAA ATGTCAGGAT
GCTCCAGACC CTATTAAAAG ATGCATATAT CAAGTTAGTT GCATGCTCTG TGCAGAGAGC
ATGTTGTATC ATTGTATGTC AGACTCAGAG GGAGATTTTT CTGATCCCTG TTCGTGTGAC
ACTAGCGACG ACAAGTTCTG CTTGCGATGG TTAGCCCTGG TAGCTTTGTC TTTCATTGTA
CCATGTATGT GCTGCTACGT CCCTTTGAGA ATGTGCCATC GCTGTGGTGA GGCATGTGGT
TGCTGTGGTG GGAAACATAA AGCTGCTGGA TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005254260.1
CDS1147..2259
Misc Feature(1)1150..1299(+)
Misc Feature(2)1918..2208(+)
Translation
Position Chain Variation Link
30 30 c, t dbSNP:578109576
31 31 c, t dbSNP:753674221
33 33 c, g dbSNP:545234930
41 41 -, a dbSNP:754835889
42 42 -, a dbSNP:778544680
94 94 a, c dbSNP:753013391
107 107 a, g dbSNP:563464823
115 115 a, g dbSNP:530873122
145 145 c, t dbSNP:758605846
178 178 -, gct dbSNP:758522996
180 180 g, t dbSNP:1522781
232 232 a, g dbSNP:560973597
244 244 c, g dbSNP:528091119
254 254 a, g dbSNP:546545058
303 303 a, g dbSNP:1522782
432 432 c, t dbSNP:193069101
443 443 -, t dbSNP:572126972
483 483 c, t dbSNP:551597076
544 544 c, g, t dbSNP:569895675
552 552 a, g dbSNP:184673190
674 674 c, t dbSNP:189438859
685 685 c, t dbSNP:567923633
755 755 g, t dbSNP:534711799
768 768 a, g dbSNP:147075186
791 791 c, t dbSNP:578173447
835 835 a, t dbSNP:746319268
856 856 c, t dbSNP:138385793
866 866 c, g dbSNP:557220249
899 899 a, g dbSNP:770231868
909 909 c, t dbSNP:556907443
934 934 a, g dbSNP:542772692
938 938 -, g dbSNP:531519324
963 963 a, c dbSNP:561044956
964 964 c, t dbSNP:573042758
1007 1007 c, t dbSNP:192699510
1049 1049 c, g dbSNP:369369431
1062 1062 g, t dbSNP:762837345
1070 1070 a, g dbSNP:564526874
1072 1072 c, t dbSNP:751783011
1076 1076 a, g dbSNP:755230054
1082 1082 g, t dbSNP:755489788
1083 1083 a, t dbSNP:781241994
1086 1086 a, c dbSNP:532105923
1087 1087 c, g dbSNP:756552268
1088 1088 c, g, t dbSNP:371200183
1098 1098 a, t dbSNP:773950720
1105 1105 c, t dbSNP:775693385
1125 1125 a, t dbSNP:200157475
1126 1126 c, t dbSNP:727504717
1127 1127 a, t dbSNP:779189071
1129 1129 a, c dbSNP:201692618
1135 1135 a, g dbSNP:369150309
1152 1152 a, t dbSNP:763749955
1153 1153 a, t dbSNP:727504170
1194 1194 c, g, t dbSNP:773943895
1196 1196 a, g dbSNP:756975029
1203 1203 a, g dbSNP:778384985
1204 1204 a, g dbSNP:749950643
1205 1205 c, t dbSNP:761473300
1214 1214 a, g dbSNP:556636985
1215 1215 a, g dbSNP:7182445
1229 1229 c, t dbSNP:754706111
1230 1230 a, g dbSNP:372791883
1235 1235 a, g dbSNP:748376033
1245 1245 a, t dbSNP:770197803
1272 1272 c, t dbSNP:773363523
1273 1273 c, t dbSNP:121434312
1274 1274 a, g dbSNP:749378713
1277 1277 a, g dbSNP:373381933
1282 1282 a, g dbSNP:774740245
1295 1295 c, t dbSNP:760132893
1297 1297 c, t dbSNP:767771754
1308 1308 c, t dbSNP:761058615
1309 1309 a, g dbSNP:764823722
1312 1312 c, t dbSNP:149883080
1320 1320 c, t dbSNP:772830013
1324 1324 a, g dbSNP:755991015
1325 1325 c, g dbSNP:762633609
1329 1329 a, g dbSNP:778868909
1332 1332 g, t dbSNP:765800186
1337 1337 a, g dbSNP:751138842
1341 1341 c, t dbSNP:12148911
1348 1348 g, t dbSNP:763971890
1350 1350 a, g dbSNP:753701682
1362 1362 c, t dbSNP:757357920
1370 1370 a, g dbSNP:373384814
1375 1375 c, g dbSNP:750421037
1387 1387 c, g, t dbSNP:758483715
1389 1389 c, t dbSNP:376944837
1394 1394 a, t dbSNP:747508368
1400 1400 a, c dbSNP:769206547
1401 1401 a, t dbSNP:781477854
1419 1419 g, t dbSNP:748550020
1422 1422 c, g dbSNP:770446191
1427 1427 a, t dbSNP:774070689
1431 1431 a, c dbSNP:759158378
1434 1434 c, t dbSNP:771673657
1435 1435 c, t dbSNP:376527959
1443 1443 a, g dbSNP:532147114
1448 1448 -, ctt dbSNP:763365429
1454 1454 c, t dbSNP:553832516
1459 1459 a, g dbSNP:143121426
1484 1484 a, g dbSNP:764135694
1485 1485 c, t dbSNP:201335683
1487 1487 c, t dbSNP:370735097
1488 1488 a, g dbSNP:202092705
1489 1489 a, c dbSNP:200142785
1491 1491 a, c, t dbSNP:764990934
1494 1494 c, t dbSNP:758617124
1502 1502 a, g dbSNP:766431294
1511 1511 c, t dbSNP:147474792
1518 1518 c, t dbSNP:756643495
1523 1523 c, t dbSNP:778144652
1531 1531 a, g dbSNP:139897054
1534 1534 a, g dbSNP:758087535
1538 1538 a, g dbSNP:779879921
1542 1542 c, g dbSNP:746708059
1543 1543 a, c dbSNP:368660900
1548 1548 c, t dbSNP:776073446
1557 1557 c, t dbSNP:149991304
1558 1558 a, g dbSNP:769630742
1561 1561 c, t dbSNP:121434316
1564 1564 a, c, t dbSNP:376699107
1565 1565 a, g dbSNP:371274407
1567 1567 c, g, t dbSNP:121434314
1569 1569 a, c dbSNP:144227155
1587 1587 a, g dbSNP:766539664
1593 1593 a, g dbSNP:774530752
1599 1599 c, g, t dbSNP:144764225
1609 1609 g, t dbSNP:201837340
1611 1611 a, c dbSNP:376226762
1620 1620 a, g dbSNP:764287445
1626 1626 c, g dbSNP:138553244
1636 1636 a, g dbSNP:762478369
1655 1655 a, g dbSNP:766152186
1689 1689 a, g dbSNP:765684707
1694 1694 a, g dbSNP:750832911
1708 1708 a, t dbSNP:121434317
1719 1719 c, t dbSNP:116667267
1745 1745 a, g dbSNP:111363743
1747 1747 g, t dbSNP:780737673
1759 1759 a, g dbSNP:752201824
1765 1765 c, t dbSNP:755557783
1778 1778 c, t dbSNP:759197561
1788 1788 a, c dbSNP:777379558
1790 1790 -, aatcagact dbSNP:778423017
1790 1790 a, c dbSNP:372665535
1799 1799 a, g dbSNP:201933663
1803 1803 a, g dbSNP:535687467
1810 1810 c, t dbSNP:554327738
1811 1811 a, g dbSNP:200974459
1812 1812 c, t dbSNP:775556490
1816 1816 c, g dbSNP:761001792
1823 1823 c, g dbSNP:540124243
1830 1830 a, g dbSNP:776909752
1844 1844 a, g dbSNP:764868020
1846 1846 a, g, t dbSNP:765841849
1850 1850 c, t dbSNP:114636635
1852 1852 a, g dbSNP:752191960
1855 1855 c, t dbSNP:375700452
1862 1862 c, t dbSNP:766814966
1863 1863 a, g, t dbSNP:140644874
1868 1868 c, t dbSNP:115440602
1878 1878 a, g dbSNP:753357510
1887 1887 -, gaagtc dbSNP:749692794
1887 1887 a, g dbSNP:369711772
1901 1901 c, g dbSNP:750890621
1909 1909 a, c, g dbSNP:756634323
1918 1918 c, t dbSNP:758102077
1919 1919 a, g dbSNP:779966392
1924 1924 c, t dbSNP:765603808
1925 1925 a, g dbSNP:146702985
1929 1929 c, t dbSNP:768937237
1930 1930 a, g dbSNP:373477920
1955 1955 a, g dbSNP:376679136
1959 1959 a, g dbSNP:770375243
1961 1961 a, g dbSNP:773833198
1968 1968 c, g, t dbSNP:3751526
1969 1969 a, c, t dbSNP:774835982
1997 1997 c, g dbSNP:760186912
2000 2000 a, t dbSNP:763738243
2002 2002 a, g dbSNP:374685699
2004 2004 a, g dbSNP:749654043
2010 2010 c, t dbSNP:756739740
2011 2011 a, g dbSNP:764768156
2013 2013 a, g dbSNP:140225135
2020 2020 a, g dbSNP:372623722
2029 2029 a, t dbSNP:779660163
2032 2032 c, t dbSNP:397517870
2052 2052 g, t dbSNP:746827242
2071 2071 c, t dbSNP:755208632
2074 2074 -, g dbSNP:771343745
2094 2094 c, t dbSNP:781585106
2100 2100 a, g dbSNP:755223172
2110 2110 a, g dbSNP:769959530
2112 2112 c, t dbSNP:773409045
2115 2115 c, t dbSNP:749745659
2116 2116 a, g dbSNP:771480941
2118 2118 -, c dbSNP:774533789
2126 2126 g, t dbSNP:779058019
2128 2128 c, t dbSNP:759969513
2132 2132 a, g dbSNP:772476060
2134 2134 g, t dbSNP:776235336
2140 2140 a, g dbSNP:761520561
2141 2141 c, t dbSNP:748390368
2146 2146 g, t dbSNP:150307819
2149 2149 a, g, t dbSNP:762735151
2150 2150 c, t dbSNP:751260227
2163 2163 c, t dbSNP:754794684
2184 2184 c, t dbSNP:202087411
2185 2185 a, g dbSNP:752958999
2197 2197 a, g dbSNP:368307475
2207 2207 a, g dbSNP:369492789
2213 2213 c, g dbSNP:749389617
2219 2219 c, t dbSNP:200871227
2222 2222 a, g dbSNP:779454951
2225 2225 g, t dbSNP:746414913
2231 2231 a, g dbSNP:772421738
2237 2237 g, t dbSNP:573603750
2243 2243 -, at dbSNP:759838497
2248 2248 a, g dbSNP:761401264
2261 2261 a, c dbSNP:769445247
2292 2292 -, ata dbSNP:772254530
2306 2306 c, t dbSNP:772560499
2307 2307 a, g dbSNP:373694127
2323 2323 c, t dbSNP:544137820
2325 2325 a, g dbSNP:533114849
2340 2340 c, t dbSNP:773453342
2364 2364 a, g dbSNP:545295358
2402 2402 a, c dbSNP:770816015
2408 2408 a, g dbSNP:776485386
2427 2427 c, t dbSNP:192826011
2432 2432 c, t dbSNP:765093939
2442 2442 a, g dbSNP:530541798
2449 2449 a, g dbSNP:377091058
2464 2464 a, g dbSNP:567404158
2485 2485 a, g dbSNP:377201217
2507 2507 c, t dbSNP:16966842
2509 2509 a, g dbSNP:547722924
2515 2515 c, t dbSNP:145373657
2524 2524 a, c dbSNP:539607759
2580 2580 a, g dbSNP:137920898
2617 2617 c, g dbSNP:569990101
2618 2618 a, g dbSNP:537079236
2619 2619 a, g dbSNP:751117768
2641 2641 c, g dbSNP:13329392
2650 2650 g, t dbSNP:555317480
2686 2686 a, t dbSNP:185290497
2704 2704 a, g dbSNP:577788958
2714 2714 c, g dbSNP:545070531
2789 2789 a, g dbSNP:756612869
2794 2794 a, g dbSNP:370660664
2823 2823 a, t dbSNP:373626511
2837 2837 -, at dbSNP:147547509
2838 2838 -, atat dbSNP:759359048
2840 2840 c, t dbSNP:541503324
2849 2849 a, c dbSNP:553150319
2851 2851 a, c dbSNP:754213247
2853 2853 a, c dbSNP:577769507
2892 2892 a, g dbSNP:118146919
2921 2921 a, g dbSNP:374443282
2924 2924 c, t dbSNP:755236404
2978 2978 a, g dbSNP:563589753
2995 2995 a, t dbSNP:188754645
3015 3015 c, t dbSNP:530810790
3017 3017 c, t dbSNP:560005063
3042 3042 c, t dbSNP:770577944
3095 3095 a, g dbSNP:192636064
3128 3128 -, att dbSNP:541409661
3152 3152 c, t dbSNP:542673653
3159 3159 c, t dbSNP:560864932
3202 3202 g, t dbSNP:183715838
3299 3299 a, g dbSNP:115568221
3318 3318 -, a dbSNP:34079057
3365 3365 c, t dbSNP:571210831
3373 3373 g, t dbSNP:141742028
3451 3451 c, t dbSNP:28536719
3453 3453 c, t dbSNP:28576519
3484 3484 c, t dbSNP:551528743
3536 3536 c, g dbSNP:779337443
3557 3557 a, g dbSNP:376795432
3567 3567 a, g dbSNP:368946709
3596 3596 a, c dbSNP:570038934
3630 3630 c, t dbSNP:373111945
3682 3682 a, g dbSNP:748337294
3711 3711 a, c dbSNP:751531938
3749 3749 g, t dbSNP:555380695
3751 3751 g, t dbSNP:199891616
3773 3773 g, t dbSNP:376829790
3775 3775 a, g dbSNP:757780122
3916 3916 -, ctat dbSNP:146972863
3918 3918 -, atct dbSNP:57589132
3918 3918 a, g dbSNP:113071870
3979 3979 c, t dbSNP:567659062
3983 3983 a, c dbSNP:1554527
3992 3992 a, g dbSNP:777972348
4002 4002 c, t dbSNP:747157552
4006 4006 a, c dbSNP:561525888
4020 4020 c, t dbSNP:553453653
4025 4025 -, aag dbSNP:546611820
4068 4068 a, g dbSNP:578008401
4071 4071 a, g dbSNP:770892084
4096 4096 a, g dbSNP:778489832
4154 4154 c, t dbSNP:538871697
4156 4156 c, t dbSNP:776375597
4277 4277 g, t dbSNP:188392935
4302 4302 a, g dbSNP:180946373
4339 4339 c, g dbSNP:759262779
4472 4472 c, t dbSNP:542935372
4506 4506 a, g dbSNP:118057877
4581 4581 a, g dbSNP:747836383
4636 4636 a, g dbSNP:572802254
4671 4671 -, t dbSNP:532033889
4679 4679 c, t dbSNP:184024152
4744 4744 a, g dbSNP:565009702
4745 4745 a, t dbSNP:374589452
4761 4761 -, tgagtaaa dbSNP:770258605
4761 4761 g, t dbSNP:532084025
4822 4822 -, at dbSNP:373432788
4829 4829 c, t dbSNP:111997668
4857 4857 a, c dbSNP:189861748
4859 4859 c, t dbSNP:775287695
4864 4864 c, t dbSNP:530967462
4870 4870 c, t dbSNP:181716461
4871 4871 a, g dbSNP:567834681
4881 4881 a, g dbSNP:138645237
4894 4894 c, g dbSNP:546662922
4945 4945 a, t dbSNP:571741901
4968 4968 c, t dbSNP:539133361
4969 4969 g, t dbSNP:557234725
4982 4982 c, t dbSNP:762569457
4987 4987 -, t dbSNP:773860286
4992 4992 a, t dbSNP:763774696
4993 4993 c, g dbSNP:773847026
5001 5001 g, t dbSNP:149336440
5011 5011 -, g dbSNP:746468942
5045 5045 c, g dbSNP:147428585
5083 5083 c, g dbSNP:761286407
5090 5090 a, g dbSNP:35490364
5107 5107 c, t dbSNP:754227753
5112 5112 a, g dbSNP:187134665
5144 5144 a, g dbSNP:79309779
5167 5167 c, t dbSNP:765645509
5203 5203 a, t dbSNP:759460441
5205 5205 a, c dbSNP:564720434
5259 5259 g, t dbSNP:753155345
5288 5288 -, gctgaaa dbSNP:553420659
5316 5316 a, g dbSNP:72725362
5326 5326 -, tt dbSNP:771536852
5337 5337 g, t dbSNP:543909086
5370 5370 c, t dbSNP:111766079
5390 5390 c, t dbSNP:373027564
5397 5397 a, g dbSNP:531030936
5423 5423 a, g dbSNP:191914374
5451 5451 a, g dbSNP:566065329
5513 5513 a, g dbSNP:561659585
5520 5520 c, t dbSNP:751824076
5572 5572 a, c, t dbSNP:536604218
5574 5574 a, g dbSNP:757217761
5582 5582 c, t dbSNP:547019744
5590 5590 a, g dbSNP:781366830
5599 5599 a, g dbSNP:571542327
5653 5653 c, t dbSNP:548164209
5654 5654 a, g dbSNP:140749542
5674 5674 a, c dbSNP:551099276
5718 5718 a, g dbSNP:764169369
5743 5743 -, tt dbSNP:774937535
5780 5780 a, g dbSNP:11634702
5811 5811 a, g dbSNP:376186397
5819 5819 a, g dbSNP:200294834
5820 5820 a, g dbSNP:181980647
5831 5831 a, g dbSNP:113753698
5840 5840 g, t dbSNP:779791635
5842 5842 a, c, g dbSNP:566802137
5850 5850 c, t dbSNP:147489619
5856 5856 -, ta dbSNP:574998297
5870 5870 a, g dbSNP:558773794
5882 5882 g, t dbSNP:148525432
5886 5886 -, a dbSNP:546844553
5904 5904 -, g dbSNP:763956861
5906 5906 g, t dbSNP:186366365
5916 5916 c, t dbSNP:761527047
5957 5957 g, t dbSNP:76527876
5960 5960 a, g dbSNP:574179000
5985 5985 a, g dbSNP:543233753
6004 6004 a, g dbSNP:142928983
6047 6047 c, g dbSNP:577731830
6049 6049 c, t dbSNP:528822175
6064 6064 a, t dbSNP:759931845
6089 6089 a, g dbSNP:8039150
6091 6091 a, g dbSNP:565528602
6100 6100 g, t dbSNP:532564269
6151 6151 g, t dbSNP:112221665
6205 6205 a, g dbSNP:8039337
6255 6255 a, g dbSNP:72725364
6301 6301 a, c dbSNP:189589145
6336 6336 a, t dbSNP:572054998
6360 6360 a, g dbSNP:182237934
6361 6361 c, t dbSNP:577333581
6395 6395 -, att dbSNP:369376164
6426 6426 a, g dbSNP:758092431
6453 6453 a, t dbSNP:777252680
6465 6465 a, g dbSNP:746558881
6490 6490 a, g dbSNP:533950389
6496 6496 a, g dbSNP:558595140
6500 6500 c, g dbSNP:570386455
6523 6523 -, a dbSNP:542372343
6541 6541 -, ggtat dbSNP:767679657
6563 6563 a, g dbSNP:537477031
6607 6607 c, t dbSNP:375141692
6631 6631 a, g dbSNP:187788660
6635 6635 g, t dbSNP:781111622
6693 6693 c, t dbSNP:750554498
6695 6695 a, g dbSNP:191601293
6710 6710 -, at dbSNP:761502926
6730 6730 a, c dbSNP:574232846
6731 6731 a, c dbSNP:541556786
6745 6745 a, g dbSNP:183379085
6752 6752 a, t dbSNP:573365424
6817 6817 -, t dbSNP:765132380
6873 6873 a, g dbSNP:749236455
6874 6874 -, t dbSNP:542785965
6921 6921 a, t dbSNP:10852019
6954 6954 c, t dbSNP:565597750
6955 6955 c, t dbSNP:151078068
6956 6956 a, g dbSNP:188784206
6976 6976 c, t dbSNP:562870172
6984 6984 a, g dbSNP:530058944
7026 7026 c, g dbSNP:141044168

Target ORF information:

RefSeq Version XM_005254203
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sprouty-related, EVH1 domain containing 1 (SPRED1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254203

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTTAAAA AAGACCTCAT TTATAATAAG GTCACTCCAA CATTTCACCA CTGGAAGATT 
GATGACAAGA AGTTTGGTCT TACGTTTCAA AGTCCTGCTG ATGCTAGGGC TTTTGATAGA
GGTATCCGAA GAGCTATAGA GGATATTTCT CAAGGATGCC CCGAATCAAA AAATGAAGCT
GAAGGGGCAG ATGACTTACA AGCAAATGAA GAGGATTCTT CCAGTTCTCT AGTGAAGGAT
CACCTTTTTC AGCAAGAGAC AGTTGTTACC AGTGAGCCTT ATAGAAGCTC AAATATAAGA
CCTTCTCCCT TTGAAGATCT GAATGCCAGA AGAGTCTACA TGCAAAGCCA AGCCAATCAG
ATAACATTTG GTCAGCCAGG CTTGGACATT CAGAGCAGAA GTATGGAATA CGTACAGCGG
CAAATATCCA AGGAATGTGG AAGCCTAAAG TCCCAAAATA GGGTCCCTTT GAAATCAATC
AGACATGTCA GCTTTCAAGA TGAGGATGAG ATTGTCAGAA TAAACCCTCG AGATATCTTA
ATACGTCGCT ATGCAGACTA CAGACATCCT GACATGTGGA AAAATGACTT GGAAAGAGAT
GATGCTGATT CCAGTATTCA GTTTTCTAAA CCAGACAGTA AAAAATCAGA CTATCTGTAC
TCTTGTGGGG ATGAGACTAA GTTAAGTTCA CCCAAAGACT CTGTGGTATT TAAGACGCAG
CCTTCCTCAT TAAAAATTAA GAAGTCAAAA CGAAGAAAAG AGGATGGTGA ACGTTCTCGC
TGCGTATACT GCCAGGAAAG GTTTAATCAT GAAGAAAATG TTAGGGGAAA ATGTCAGGAT
GCTCCAGACC CTATTAAAAG ATGCATATAT CAAGTTAGTT GCATGCTCTG TGCAGAGAGC
ATGTTGTATC ATTGTATGTC AGACTCAGAG GGAGATTTTT CTGATCCCTG TTCGTGTGAC
ACTAGCGACG ACAAGTTCTG CTTGCGATGG TTAGCCCTGG TAGCTTTGTC TTTCATTGTA
CCATGTATGT GCTGCTACGT CCCTTTGAGA ATGTGCCATC GCTGTGGTGA GGCATGTGGT
TGCTGTGGTG GGAAACATAA AGCTGCTGGA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77141
Accession Version XM_011521290.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA
GCTGCTGGAT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011519592.1
CDS119..1390
Misc Feature(1)119..430(+)
Misc Feature(2)119..328(+)
Misc Feature(3)1049..1339(+)
Translation
Position Chain Variation Link
6 6 c, t dbSNP:563925156
17 17 a, g dbSNP:183753466
23 23 -, g dbSNP:71417101
24 24 g, t dbSNP:372467951
60 60 a, g dbSNP:748512713
79 79 -, t dbSNP:767219677
80 80 c, t dbSNP:79328043
97 97 c, t dbSNP:764234046
98 98 a, g dbSNP:753924528
102 102 a, g, t dbSNP:757726772
107 107 c, g dbSNP:750777752
108 108 a, g dbSNP:758855467
113 113 c, g dbSNP:780614363
115 115 c, g dbSNP:367745143
124 124 c, t dbSNP:755788325
125 125 c, t dbSNP:121434313
174 174 a, c, g dbSNP:781390048
178 178 c, t dbSNP:748759533
179 179 a, g dbSNP:147204964
186 186 a, t dbSNP:121434318
189 189 g, t dbSNP:778847063
196 196 c, t dbSNP:745859168
198 198 c, t dbSNP:771825294
203 203 c, t dbSNP:148646547
218 218 c, t dbSNP:775334473
223 223 g, t dbSNP:760939387
227 227 a, t dbSNP:768775931
232 232 c, t dbSNP:397517871
233 233 a, g dbSNP:761830220
236 236 c, t dbSNP:765788978
237 237 a, g, t dbSNP:750686148
245 245 c, t dbSNP:121434315
246 246 a, g dbSNP:372205720
251 251 a, c dbSNP:751923342
266 266 a, g dbSNP:369150309
283 283 a, t dbSNP:763749955
284 284 a, t dbSNP:727504170
325 325 c, g, t dbSNP:773943895
327 327 a, g dbSNP:756975029
334 334 a, g dbSNP:778384985
335 335 a, g dbSNP:749950643
336 336 c, t dbSNP:761473300
345 345 a, g dbSNP:556636985
346 346 a, g dbSNP:7182445
360 360 c, t dbSNP:754706111
361 361 a, g dbSNP:372791883
366 366 a, g dbSNP:748376033
376 376 a, t dbSNP:770197803
403 403 c, t dbSNP:773363523
404 404 c, t dbSNP:121434312
405 405 a, g dbSNP:749378713
408 408 a, g dbSNP:373381933
413 413 a, g dbSNP:774740245
426 426 c, t dbSNP:760132893
428 428 c, t dbSNP:767771754
439 439 c, t dbSNP:761058615
440 440 a, g dbSNP:764823722
443 443 c, t dbSNP:149883080
451 451 c, t dbSNP:772830013
455 455 a, g dbSNP:755991015
456 456 c, g dbSNP:762633609
460 460 a, g dbSNP:778868909
463 463 g, t dbSNP:765800186
468 468 a, g dbSNP:751138842
472 472 c, t dbSNP:12148911
479 479 g, t dbSNP:763971890
481 481 a, g dbSNP:753701682
493 493 c, t dbSNP:757357920
501 501 a, g dbSNP:373384814
506 506 c, g dbSNP:750421037
518 518 c, g, t dbSNP:758483715
520 520 c, t dbSNP:376944837
525 525 a, t dbSNP:747508368
531 531 a, c dbSNP:769206547
532 532 a, t dbSNP:781477854
550 550 g, t dbSNP:748550020
553 553 c, g dbSNP:770446191
558 558 a, t dbSNP:774070689
562 562 a, c dbSNP:759158378
565 565 c, t dbSNP:771673657
566 566 c, t dbSNP:376527959
574 574 a, g dbSNP:532147114
579 579 -, ctt dbSNP:763365429
585 585 c, t dbSNP:553832516
590 590 a, g dbSNP:143121426
615 615 a, g dbSNP:764135694
616 616 c, t dbSNP:201335683
618 618 c, t dbSNP:370735097
619 619 a, g dbSNP:202092705
620 620 a, c dbSNP:200142785
622 622 a, c, t dbSNP:764990934
625 625 c, t dbSNP:758617124
633 633 a, g dbSNP:766431294
642 642 c, t dbSNP:147474792
649 649 c, t dbSNP:756643495
654 654 c, t dbSNP:778144652
662 662 a, g dbSNP:139897054
665 665 a, g dbSNP:758087535
669 669 a, g dbSNP:779879921
673 673 c, g dbSNP:746708059
674 674 a, c dbSNP:368660900
679 679 c, t dbSNP:776073446
688 688 c, t dbSNP:149991304
689 689 a, g dbSNP:769630742
692 692 c, t dbSNP:121434316
695 695 a, c, t dbSNP:376699107
696 696 a, g dbSNP:371274407
698 698 c, g, t dbSNP:121434314
700 700 a, c dbSNP:144227155
718 718 a, g dbSNP:766539664
724 724 a, g dbSNP:774530752
730 730 c, g, t dbSNP:144764225
740 740 g, t dbSNP:201837340
742 742 a, c dbSNP:376226762
751 751 a, g dbSNP:764287445
757 757 c, g dbSNP:138553244
767 767 a, g dbSNP:762478369
786 786 a, g dbSNP:766152186
820 820 a, g dbSNP:765684707
825 825 a, g dbSNP:750832911
839 839 a, t dbSNP:121434317
850 850 c, t dbSNP:116667267
876 876 a, g dbSNP:111363743
878 878 g, t dbSNP:780737673
890 890 a, g dbSNP:752201824
896 896 c, t dbSNP:755557783
909 909 c, t dbSNP:759197561
919 919 a, c dbSNP:777379558
921 921 -, aatcagact dbSNP:778423017
921 921 a, c dbSNP:372665535
930 930 a, g dbSNP:201933663
934 934 a, g dbSNP:535687467
941 941 c, t dbSNP:554327738
942 942 a, g dbSNP:200974459
943 943 c, t dbSNP:775556490
947 947 c, g dbSNP:761001792
954 954 c, g dbSNP:540124243
961 961 a, g dbSNP:776909752
975 975 a, g dbSNP:764868020
977 977 a, g, t dbSNP:765841849
981 981 c, t dbSNP:114636635
983 983 a, g dbSNP:752191960
986 986 c, t dbSNP:375700452
993 993 c, t dbSNP:766814966
994 994 a, g, t dbSNP:140644874
999 999 c, t dbSNP:115440602
1009 1009 a, g dbSNP:753357510
1018 1018 -, gaagtc dbSNP:749692794
1018 1018 a, g dbSNP:369711772
1032 1032 c, g dbSNP:750890621
1040 1040 a, c, g dbSNP:756634323
1049 1049 c, t dbSNP:758102077
1050 1050 a, g dbSNP:779966392
1055 1055 c, t dbSNP:765603808
1056 1056 a, g dbSNP:146702985
1060 1060 c, t dbSNP:768937237
1061 1061 a, g dbSNP:373477920
1086 1086 a, g dbSNP:376679136
1090 1090 a, g dbSNP:770375243
1092 1092 a, g dbSNP:773833198
1099 1099 c, g, t dbSNP:3751526
1100 1100 a, c, t dbSNP:774835982
1128 1128 c, g dbSNP:760186912
1131 1131 a, t dbSNP:763738243
1133 1133 a, g dbSNP:374685699
1135 1135 a, g dbSNP:749654043
1141 1141 c, t dbSNP:756739740
1142 1142 a, g dbSNP:764768156
1144 1144 a, g dbSNP:140225135
1151 1151 a, g dbSNP:372623722
1160 1160 a, t dbSNP:779660163
1163 1163 c, t dbSNP:397517870
1183 1183 g, t dbSNP:746827242
1202 1202 c, t dbSNP:755208632
1205 1205 -, g dbSNP:771343745
1225 1225 c, t dbSNP:781585106
1231 1231 a, g dbSNP:755223172
1241 1241 a, g dbSNP:769959530
1243 1243 c, t dbSNP:773409045
1246 1246 c, t dbSNP:749745659
1247 1247 a, g dbSNP:771480941
1249 1249 -, c dbSNP:774533789
1257 1257 g, t dbSNP:779058019
1259 1259 c, t dbSNP:759969513
1263 1263 a, g dbSNP:772476060
1265 1265 g, t dbSNP:776235336
1271 1271 a, g dbSNP:761520561
1272 1272 c, t dbSNP:748390368
1277 1277 g, t dbSNP:150307819
1280 1280 a, g, t dbSNP:762735151
1281 1281 c, t dbSNP:751260227
1294 1294 c, t dbSNP:754794684
1315 1315 c, t dbSNP:202087411
1316 1316 a, g dbSNP:752958999
1328 1328 a, g dbSNP:368307475
1338 1338 a, g dbSNP:369492789
1344 1344 c, g