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DAZL cDNA ORF clone, Homo sapiens (human)

Gene Symbol DAZL
Entrez Gene ID 1618
Full Name deleted in azoospermia-like
Synonyms DAZH, DAZL1, DAZLA, SPGYLA
General protein information
Preferred Names
deleted in azoospermia-like
Names
deleted in azoospermia-like
DAZ homolog
DAZ-like autosomal
SPGY-like-autosomal
deleted in azoospermia-like 1
germline specific RNA binding protein
spermatogenesis gene on the Y-like autosomal
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p24.3

Summary The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]. lac of sum
Disorder MIM:

601486

Disorder Html: {Spermatogenic failure, susceptibility to} (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001351 NP_001342 deleted in azoospermia-like isoform 2
NM_001190811 NP_001177740 deleted in azoospermia-like isoform 1



Homo sapiens (human) DAZL NP_001177740.1
Pan troglodytes (chimpanzee) DAZL XP_516314.3
Canis lupus familiaris (dog) DAZL XP_534251.3
Bos taurus (cattle) DAZL NP_001075194.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DAZL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAZL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27425
NM_001351 Homo sapiens deleted in azoospermia-like (DAZL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu26820 NM_001190811 Homo sapiens deleted in azoospermia-like (DAZL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27425
Clone ID Related Accession (Same CDS sequence) NM_001351
Accession Version NM_001351.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product deleted in azoospermia-like isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB080081.1, BC027595.2 and BX648638.1. This sequence is a reference standard in the RefSeqGene project. On Jun 30, 2010 this sequence version replaced gi:25121977. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U66078.1, U65918.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTACTG CAAATCCTGA AACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG 
TCCTCATCAG CTGCAACCAG CCAAGGCTAT ATTTTACCAG AAGGCAAAAT CATGCCAAAC
ACTGTTTTTG TTGGAGGAAT TGATGTTAGG ATGGATGAAA CTGAGATTAG AAGCTTCTTT
GCTAGATATG GTTCAGTGAA AGAAGTGAAG ATAATCACTG ATCGAACTGG TGTGTCCAAA
GGCTATGGAT TTGTTTCATT TTTTAATGAC GTGGATGTGC AGAAGATAGT AGAATCACAG
ATAAATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAATTTATGT
GCTTATCATG TGCAGCCACG TCCTTTGGTT TTTAATCATC CTCCTCCACC ACAGTTTCAG
AATGTCTGGA CTAATCCAAA CACTGAAACT TATATGCAGC CCACAACCAC GATGAATCCT
ATAACTCAGT ATGTTCAGGC ATATCCTACT TACCCAAATT CACCAGTTCA GGTCATCACT
GGATATCAGT TGCCTGTATA TAATTATCAG ATGCCACCAC AGTGGCCTGT TGGGGAGCAA
AGGAGCTATG TTGTACCTCC GGCTTATTCA GCTGTTAACT ACCACTGTAA TGAAGTTGAT
CCAGGAGCTG AAGTTGTGCC AAATGAATGT TCAGTTCATG AAGCTACTCC ACCCTCTGGA
AATGGCCCAC AAAAGAAATC TGTGGACCGA AGCATACAAA CGGTGGTATC TTGTCTGTTT
AATCCAGAGA ACAGACTGAG AAACTCTGTT GTTACTCAAG ATGACTACTT CAAGGATAAA
AGAGTGCATC ACTTTAGAAG AAGTCGGGCA ATGCTTAAAT CTGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001342.2
CDS295..1182
Misc Feature(1)55..57(+)
Misc Feature(2)397..642(+)
Misc Feature(3)406..642(+)
Misc Feature(4)415..624(+)
Misc Feature(5)451..528(+)
Misc Feature(6)532..690(+)
Exon (1)1..297
Gene:DAZL
Gene Synonym:
Exon (2)298..444
Gene:DAZL
Gene Synonym:
Exon (3)445..536
Gene:DAZL
Gene Synonym:
Exon (4)537..588
Gene:DAZL
Gene Synonym:
Exon (5)589..652
Gene:DAZL
Gene Synonym:
Exon (6)653..792
Gene:DAZL
Gene Synonym:
Exon (7)793..864
Gene:DAZL
Gene Synonym:
Exon (8)865..915
Gene:DAZL
Gene Synonym:
Exon (9)916..1029
Gene:DAZL
Gene Synonym:
Exon (10)1030..1128
Gene:DAZL
Gene Synonym:
Exon (11)1129..3050
Gene:DAZL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001351
Organism Homo sapiens (human)
Definition Homo sapiens deleted in azoospermia-like (DAZL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001351

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTACTG CAAATCCTGA AACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG 
TCCTCATCAG CTGCAACCAG CCAAGGCTAT ATTTTACCAG AAGGCAAAAT CATGCCAAAC
ACTGTTTTTG TTGGAGGAAT TGATGTTAGG ATGGATGAAA CTGAGATTAG AAGCTTCTTT
GCTAGATATG GTTCAGTGAA AGAAGTGAAG ATAATCACTG ATCGAACTGG TGTGTCCAAA
GGCTATGGAT TTGTTTCATT TTTTAATGAC GTGGATGTGC AGAAGATAGT AGAATCACAG
ATAAATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAATTTATGT
GCTTATCATG TGCAGCCACG TCCTTTGGTT TTTAATCATC CTCCTCCACC ACAGTTTCAG
AATGTCTGGA CTAATCCAAA CACTGAAACT TATATGCAGC CCACAACCAC GATGAATCCT
ATAACTCAGT ATGTTCAGGC ATATCCTACT TACCCAAATT CACCAGTTCA GGTCATCACT
GGATATCAGT TGCCTGTATA TAATTATCAG ATGCCACCAC AGTGGCCTGT TGGGGAGCAA
AGGAGCTATG TTGTACCTCC GGCTTATTCA GCTGTTAACT ACCACTGTAA TGAAGTTGAT
CCAGGAGCTG AAGTTGTGCC AAATGAATGT TCAGTTCATG AAGCTACTCC ACCCTCTGGA
AATGGCCCAC AAAAGAAATC TGTGGACCGA AGCATACAAA CGGTGGTATC TTGTCTGTTT
AATCCAGAGA ACAGACTGAG AAACTCTGTT GTTACTCAAG ATGACTACTT CAAGGATAAA
AGAGTGCATC ACTTTAGAAG AAGTCGGGCA ATGCTTAAAT CTGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26820
Clone ID Related Accession (Same CDS sequence) NM_001190811
Accession Version NM_001190811.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 948bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product deleted in azoospermia-like isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303026.1, AK310341.1 and BX648638.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648638.1, AK310341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGCTC CCTCGTGTGG CGGCGACAGA AAAGCTCGCC TGACGCCATC TTTGCCGCAC 
GAGTCTACTG CAAATCCTGA AACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG
TCCTCATCAG CTGCAACCAG CCAAGGCTAT ATTTTACCAG AAGGCAAAAT CATGCCAAAC
ACTGTTTTTG TTGGAGGAAT TGATGTTAGG ATGGATGAAA CTGAGATTAG AAGCTTCTTT
GCTAGATATG GTTCAGTGAA AGAAGTGAAG ATAATCACTG ATCGAACTGG TGTGTCCAAA
GGCTATGGAT TTGTTTCATT TTTTAATGAC GTGGATGTGC AGAAGATAGT AGAATCACAG
ATAAATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAATTTATGT
GCTTATCATG TGCAGCCACG TCCTTTGGTT TTTAATCATC CTCCTCCACC ACAGTTTCAG
AATGTCTGGA CTAATCCAAA CACTGAAACT TATATGCAGC CCACAACCAC GATGAATCCT
ATAACTCAGT ATGTTCAGGC ATATCCTACT TACCCAAATT CACCAGTTCA GGTCATCACT
GGATATCAGT TGCCTGTATA TAATTATCAG ATGCCACCAC AGTGGCCTGT TGGGGAGCAA
AGGAGCTATG TTGTACCTCC GGCTTATTCA GCTGTTAACT ACCACTGTAA TGAAGTTGAT
CCAGGAGCTG AAGTTGTGCC AAATGAATGT TCAGTTCATG AAGCTACTCC ACCCTCTGGA
AATGGCCCAC AAAAGAAATC TGTGGACCGA AGCATACAAA CGGTGGTATC TTGTCTGTTT
AATCCAGAGA ACAGACTGAG AAACTCTGTT GTTACTCAAG ATGACTACTT CAAGGATAAA
AGAGTGCATC ACTTTAGAAG AAGTCGGGCA ATGCTTAAAT CTGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001177740.1
CDS289..1236
Misc Feature(1)205..207(+)
Misc Feature(2)451..696(+)
Misc Feature(3)460..696(+)
Misc Feature(4)469..678(+)
Misc Feature(5)505..582(+)
Exon (1)1..351
Gene:DAZL
Gene Synonym:
Exon (2)352..498
Gene:DAZL
Gene Synonym:
Exon (3)499..590
Gene:DAZL
Gene Synonym:
Exon (4)591..642
Gene:DAZL
Gene Synonym:
Exon (5)643..706
Gene:DAZL
Gene Synonym:
Exon (6)707..846
Gene:DAZL
Gene Synonym:
Exon (7)847..918
Gene:DAZL
Gene Synonym:
Exon (8)919..969
Gene:DAZL
Gene Synonym:
Exon (9)970..1083
Gene:DAZL
Gene Synonym:
Exon (10)1084..1182
Gene:DAZL
Gene Synonym:
Exon (11)1183..3104
Gene:DAZL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001190811
Organism Homo sapiens (human)
Definition Homo sapiens deleted in azoospermia-like (DAZL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001190811

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGCGGCTC CCTCGTGTGG CGGCGACAGA AAAGCTCGCC TGACGCCATC TTTGCCGCAC 
GAGTCTACTG CAAATCCTGA AACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG
TCCTCATCAG CTGCAACCAG CCAAGGCTAT ATTTTACCAG AAGGCAAAAT CATGCCAAAC
ACTGTTTTTG TTGGAGGAAT TGATGTTAGG ATGGATGAAA CTGAGATTAG AAGCTTCTTT
GCTAGATATG GTTCAGTGAA AGAAGTGAAG ATAATCACTG ATCGAACTGG TGTGTCCAAA
GGCTATGGAT TTGTTTCATT TTTTAATGAC GTGGATGTGC AGAAGATAGT AGAATCACAG
ATAAATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAATTTATGT
GCTTATCATG TGCAGCCACG TCCTTTGGTT TTTAATCATC CTCCTCCACC ACAGTTTCAG
AATGTCTGGA CTAATCCAAA CACTGAAACT TATATGCAGC CCACAACCAC GATGAATCCT
ATAACTCAGT ATGTTCAGGC ATATCCTACT TACCCAAATT CACCAGTTCA GGTCATCACT
GGATATCAGT TGCCTGTATA TAATTATCAG ATGCCACCAC AGTGGCCTGT TGGGGAGCAA
AGGAGCTATG TTGTACCTCC GGCTTATTCA GCTGTTAACT ACCACTGTAA TGAAGTTGAT
CCAGGAGCTG AAGTTGTGCC AAATGAATGT TCAGTTCATG AAGCTACTCC ACCCTCTGGA
AATGGCCCAC AAAAGAAATC TGTGGACCGA AGCATACAAA CGGTGGTATC TTGTCTGTTT
AATCCAGAGA ACAGACTGAG AAACTCTGTT GTTACTCAAG ATGACTACTT CAAGGATAAA
AGAGTGCATC ACTTTAGAAG AAGTCGGGCA ATGCTTAAAT CTGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Altered DNA methylation patterns of the H19 differentially methylated region and the DAZL gene promoter are associated with defective human sperm
PLoS ONE 8 (8), E71215 (2013)
Li B, Li JB, Xiao XF, Ma YF, Wang J, Liang XX, Zhao HX, Jiang F, Yao YQ and Wang XH.


book

Identification of nine new susceptibility loci for testicular cancer, including variants near DAZL and PRDM14
Nat. Genet. 45 (6), 686-689 (2013)
Ruark E, Seal S, McDonald H, Zhang F, Elliot A, Lau K, Perdeaux E, Rapley E, Eeles R, Peto J, Kote-Jarai Z, Muir K, Nsengimana J, Shipley J, Bishop DT, Stratton MR, Easton DF, Huddart RA, Rahman N and Turnbull C.


book

[DAZL gene polymorphisms and astheno-teratozoospermia]
Zhonghua Nan Ke Xue 19 (4), 311-314 (2013)
Ye LW, Yu QF, Yang XX, Li JP, Wu XQ, Zhang YH and Mao XM.


book

Nodal, Nanog, DAZL and SMAD gene expression in human amniotic fluid stem cells
J Stem Cells 8 (1), 17-23 (2013)
Stefanidis K, Pergialiotis V, Christakis D, Loutradis D and Antsaklis A.


book

A single-nucleotide polymorphism of the DAZL gene promoter confers susceptibility to spermatogenic failure in the Taiwanese Han
Hum. Reprod. 27 (9), 2857-2865 (2012)
Teng YN, Chang YP, Tseng JT, Kuo PH, Lee IW, Lee MS and Kuo PL.


book

A putative human male infertility gene DAZLA: genomic structure and methylation status
Mol. Hum. Reprod. 3 (8), 705-708 (1997)
Chai NN, Phillips A, Fernandez A and Yen PH.


book

Gene sequence, localization, and evolutionary conservation of DAZLA, a candidate male sterility gene
Genomics 41 (2), 227-235 (1997)
Seboun E, Barbaux S, Bourgeron T, Nishi S, Agulnik A, Egashira M, Nikkawa N, Bishop C, Fellous M, McElreavey K and Kasahara M.


book

The human autosomal gene DAZLA: testis specificity and a candidate for male infertility
Hum. Mol. Genet. 5 (12), 2013-2017 (1996)
Yen PH, Chai NN and Salido EC.


book

A SPGY copy homologous to the mouse gene Dazla and the Drosophila gene boule is autosomal and expressed only in the human male gonad
Hum. Mol. Genet. 5 (12), 2005-2011 (1996)
Shan Z, Hirschmann P, Seebacher T, Edelmann A, Jauch A, Morell J, Urbitsch P and Vogt PH.


book

The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that was transposed, repeatedly amplified and pruned
Nat. Genet. 14 (3), 292-299 (1996)
Saxena R, Brown LG, Hawkins T, Alagappan RK, Skaletsky H, Reeve MP, Reijo R, Rozen S, Dinulos MB, Disteche CM and Page DC.


 
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