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DDB2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol DDB2
Entrez Gene ID 1643
Full Name damage-specific DNA binding protein 2, 48kDa
Synonyms DDBB, UV-DDB2
General protein information
Preferred Names
DNA damage-binding protein 2
Names
DNA damage-binding protein 2
UV-DDB 2
DDB p48 subunit
UV-damaged DNA-binding protein 2
damage-specific DNA-binding protein 2
xeroderma pigmentosum group E protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p12-p11

Summary This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]. lac of sum
Disorder MIM:

600811

Disorder Html: Xeroderma pigmentosum, group E, DDB-negative subtype, 278740 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000107 NP_000098 DNA damage-binding protein 2
NM_001300734 NP_001287663 DNA damage-binding protein 2 isoform D1



Homo sapiens (human) DDB2 NP_000098.1
Pan troglodytes (chimpanzee) DDB2 XP_001167874.2
Macaca mulatta (Rhesus monkey) DDB2 XP_001109935.1
Canis lupus familiaris (dog) DDB2 XP_540746.3
Bos taurus (cattle) DDB2 NP_001069256.1
Mus musculus (house mouse) Ddb2 NP_082395.2
Rattus norvegicus (Norway rat) Ddb2 NP_001258275.1
Gallus gallus (chicken) DDB2 NP_001034390.1
Danio rerio (zebrafish) ddb2 NP_001076530.1
Arabidopsis thaliana (thale cress) DDB2 NP_200684.2
Xenopus (Silurana) tropicalis (western clawed frog) ddb2 NP_001008203.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19345
NM_000107 Homo sapiens damage-specific DNA binding protein 2, 48kDa (DDB2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu44784 NM_001300734 Homo sapiens damage-specific DNA binding protein 2, 48kDa (DDB2), transcript variant D1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19345
Clone ID Related Accession (Same CDS sequence) NM_000107 , XM_006718161
Accession Version NM_000107.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA damage-binding protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053816.1, U18300.1 and AA278953.1. This sequence is a reference standard in the RefSeqGene project. On Jan 2, 2008 this sequence version replaced gi:4557514. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U18300.1, BC000093.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCTCCCA AGAAACGCCC AGAAACCCAG AAGACCTCCG AGATTGTATT ACGCCCCAGG 
AACAAGAGGA GCAGGAGTCC CCTGGAGCTG GAGCCCGAGG CCAAGAAGCT CTGTGCGAAG
GGCTCCGGTC CTAGCAGAAG ATGTGACTCA GACTGCCTCT GGGTGGGGCT GGCTGGCCCA
CAGATCCTGC CACCATGCCG CAGCATCGTC AGGACCCTCC ACCAGCATAA GCTGGGCAGA
GCTTCCTGGC CATCTGTCCA GCAGGGGCTC CAGCAGTCCT TTTTGCACAC TCTGGATTCT
TACCGGATAT TACAAAAGGC TGCCCCCTTT GACAGGAGGG CTACATCCTT GGCGTGGCAC
CCAACTCACC CCAGCACCGT GGCTGTGGGT TCCAAAGGGG GAGATATCAT GCTCTGGAAT
TTTGGCATCA AGGACAAACC CACCTTCATC AAAGGGATTG GAGCTGGAGG GAGCATCACT
GGGCTGAAGT TTAACCCTCT CAATACCAAC CAGTTTTACG CCTCCTCAAT GGAGGGAACA
ACTAGGCTGC AAGACTTTAA AGGCAACATT CTACGAGTTT TTGCCAGCTC AGACACCATC
AACATCTGGT TTTGTAGCCT GGATGTGTCT GCTAGTAGCC GAATGGTGGT CACAGGAGAC
AACGTGGGGA ACGTGATCCT GCTGAACATG GACGGCAAAG AGCTTTGGAA TCTCAGAATG
CACAAAAAGA AAGTGACGCA TGTGGCCCTG AACCCATGCT GTGATTGGTT CCTGGCCACA
GCCTCCGTAG ATCAAACAGT GAAAATTTGG GACCTGCGCC AGGTTAGAGG GAAAGCCAGC
TTCCTCTACT CGCTGCCGCA CAGGCATCCT GTCAACGCAG CTTGTTTCAG TCCCGATGGA
GCCCGGCTCC TGACCACGGA CCAGAAGAGC GAGATCCGAG TTTACTCTGC TTCCCAGTGG
GACTGCCCCC TGGGCCTGAT CCCGCACCCT CACCGTCACT TCCAGCACCT CACACCCATC
AAGGCAGCCT GGCATCCTCG CTACAACCTC ATTGTTGTGG GCCGATACCC AGATCCTAAT
TTCAAAAGTT GTACCCCTTA TGAATTGAGG ACGATCGACG TGTTCGATGG AAACTCAGGG
AAGATGATGT GTCAGCTCTA TGACCCAGAA TCTTCTGGCA TCAGTTCGCT TAATGAATTC
AATCCCATGG GGGACACGCT GGCCTCTGCA ATGGGTTACC ACATTCTCAT CTGGAGCCAG
GAGGAAGCCA GGACACGGAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000098.1
CDS196..1479
Misc Feature(1)163..165(+)
Misc Feature(2)265..267(+)
Misc Feature(3)265..267(+)
Misc Feature(4)271..273(+)
Misc Feature(5)271..273(+)
Misc Feature(6)397..>1452(+)
Misc Feature(7)397..432(+)
Misc Feature(8)454..489(+)
Misc Feature(9)538..1452(+)
Misc Feature(10)541..648(+)
Misc Feature(11)580..1452(+)
Misc Feature(12)670..777(+)
Misc Feature(13)802..909(+)
Misc Feature(14)925..1056(+)
Misc Feature(15)961..1017(+)
Misc Feature(16)1063..1182(+)
Misc Feature(17)1195..1203(+)
Misc Feature(18)1222..1353(+)
Misc Feature(19)1381..1455(+)
Exon (1)1..322
Gene:DDB2
Gene Synonym:
Exon (2)323..459
Gene:DDB2
Gene Synonym:
Exon (3)460..651
Gene:DDB2
Gene Synonym:
Exon (4)652..797
Gene:DDB2
Gene Synonym:
Exon (5)798..897
Gene:DDB2
Gene Synonym:
Exon (6)898..1075
Gene:DDB2
Gene Synonym:
Exon (7)1076..1218
Gene:DDB2
Gene Synonym:
Exon (8)1219..1383
Gene:DDB2
Gene Synonym:
Exon (9)1384..1429
Gene:DDB2
Gene Synonym:
Exon (10)1430..1848
Gene:DDB2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000107
Organism Homo sapiens (human)
Definition Homo sapiens damage-specific DNA binding protein 2, 48kDa (DDB2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000107

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCTCCCA AGAAACGCCC AGAAACCCAG AAGACCTCCG AGATTGTATT ACGCCCCAGG 
AACAAGAGGA GCAGGAGTCC CCTGGAGCTG GAGCCCGAGG CCAAGAAGCT CTGTGCGAAG
GGCTCCGGTC CTAGCAGAAG ATGTGACTCA GACTGCCTCT GGGTGGGGCT GGCTGGCCCA
CAGATCCTGC CACCATGCCG CAGCATCGTC AGGACCCTCC ACCAGCATAA GCTGGGCAGA
GCTTCCTGGC CATCTGTCCA GCAGGGGCTC CAGCAGTCCT TTTTGCACAC TCTGGATTCT
TACCGGATAT TACAAAAGGC TGCCCCCTTT GACAGGAGGG CTACATCCTT GGCGTGGCAC
CCAACTCACC CCAGCACCGT GGCTGTGGGT TCCAAAGGGG GAGATATCAT GCTCTGGAAT
TTTGGCATCA AGGACAAACC CACCTTCATC AAAGGGATTG GAGCTGGAGG GAGCATCACT
GGGCTGAAGT TTAACCCTCT CAATACCAAC CAGTTTTACG CCTCCTCAAT GGAGGGAACA
ACTAGGCTGC AAGACTTTAA AGGCAACATT CTACGAGTTT TTGCCAGCTC AGACACCATC
AACATCTGGT TTTGTAGCCT GGATGTGTCT GCTAGTAGCC GAATGGTGGT CACAGGAGAC
AACGTGGGGA ACGTGATCCT GCTGAACATG GACGGCAAAG AGCTTTGGAA TCTCAGAATG
CACAAAAAGA AAGTGACGCA TGTGGCCCTG AACCCATGCT GTGATTGGTT CCTGGCCACA
GCCTCCGTAG ATCAAACAGT GAAAATTTGG GACCTGCGCC AGGTTAGAGG GAAAGCCAGC
TTCCTCTACT CGCTGCCGCA CAGGCATCCT GTCAACGCAG CTTGTTTCAG TCCCGATGGA
GCCCGGCTCC TGACCACGGA CCAGAAGAGC GAGATCCGAG TTTACTCTGC TTCCCAGTGG
GACTGCCCCC TGGGCCTGAT CCCGCACCCT CACCGTCACT TCCAGCACCT CACACCCATC
AAGGCAGCCT GGCATCCTCG CTACAACCTC ATTGTTGTGG GCCGATACCC AGATCCTAAT
TTCAAAAGTT GTACCCCTTA TGAATTGAGG ACGATCGACG TGTTCGATGG AAACTCAGGG
AAGATGATGT GTCAGCTCTA TGACCCAGAA TCTTCTGGCA TCAGTTCGCT TAATGAATTC
AATCCCATGG GGGACACGCT GGCCTCTGCA ATGGGTTACC ACATTCTCAT CTGGAGCCAG
GAGGAAGCCA GGACACGGAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44784
Clone ID Related Accession (Same CDS sequence) NM_001300734
Accession Version NM_001300734.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA damage-binding protein 2 isoform D1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053816.1, AB107037.1 and AA278953.1. On Jul 11, 2014 this sequence version replaced gi:578820369. Transcript Variant: This variant (D1) lacks four consecutive alternate exons compared to variant WT, without a reading frame change. The resulting isoform (D1) has the same N- and C-termini but is shorter compared to isoform WT. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB107037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCCA AGAAACGCCC AGAAACCCAG AAGACCTCCG AGATTGTATT ACGCCCCAGG 
AACAAGAGGA GCAGGAGTCC CCTGGAGCTG GAGCCCGAGG CCAAGAAGCT CTGTGCGAAG
GGCTCCGGTC CTAGCAGAAG ATGTGACTCA GACTGCCTCT GGGTGGGGCT GGCTGGCCCA
CAGATCCTGC CACCATGCCG CAGCATCGTC AGGACCCTCC ACCAGCATAA GCTGGGCAGA
GCTTCCTGGC CATCTGTCCA GCAGGGGCTC CAGCAGTCCT TTTTGCACAC TCTGGATTCT
TACCGGATAT TACAAAAGGC TGCCCCCTTT GACAGGAGGG CTACATCCTT GGCGTGGCAC
CCAACTCACC CCAGCACCGT GGCTGTGGGT TCCAAAGGGG GAGATATCAT GCTCTGGAAT
TTTGGCATCA AGGACAAACC CACCTTCATC AAAGGGGCAG CCTGGCATCC TCGCTACAAC
CTCATTGTTG TGGGCCGATA CCCAGATCCT AATTTCAAAA GTTGTACCCC TTATGAATTG
AGGACGATCG ACGTGTTCGA TGGAAACTCA GGGAAGATGA TGTGTCAGCT CTATGACCCA
GAATCTTCTG GCATCAGTTC GCTTAATGAA TTCAATCCCA TGGGGGACAC GCTGGCCTCT
GCAATGGGTT ACCACATTCT CATCTGGAGC CAGGAGGAAG CCAGGACACG GAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001287663.1
CDS196..912
Misc Feature(1)163..165(+)
Misc Feature(2)265..267(+)
Misc Feature(3)271..273(+)
Misc Feature(4)397..432(+)
Misc Feature(5)454..489(+)
Misc Feature(6)538..>885(+)
Misc Feature(7)541..648(+)
Exon (1)1..322
Gene:DDB2
Gene Synonym:
Exon (2)323..459
Gene:DDB2
Gene Synonym:
Exon (3)460..651
Gene:DDB2
Gene Synonym:
Exon (4)652..816
Gene:DDB2
Gene Synonym:
Exon (5)817..862
Gene:DDB2
Gene Synonym:
Exon (6)863..1281
Gene:DDB2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001300734
Organism Homo sapiens (human)
Definition Homo sapiens damage-specific DNA binding protein 2, 48kDa (DDB2), transcript variant D1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300734

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCCA AGAAACGCCC AGAAACCCAG AAGACCTCCG AGATTGTATT ACGCCCCAGG 
AACAAGAGGA GCAGGAGTCC CCTGGAGCTG GAGCCCGAGG CCAAGAAGCT CTGTGCGAAG
GGCTCCGGTC CTAGCAGAAG ATGTGACTCA GACTGCCTCT GGGTGGGGCT GGCTGGCCCA
CAGATCCTGC CACCATGCCG CAGCATCGTC AGGACCCTCC ACCAGCATAA GCTGGGCAGA
GCTTCCTGGC CATCTGTCCA GCAGGGGCTC CAGCAGTCCT TTTTGCACAC TCTGGATTCT
TACCGGATAT TACAAAAGGC TGCCCCCTTT GACAGGAGGG CTACATCCTT GGCGTGGCAC
CCAACTCACC CCAGCACCGT GGCTGTGGGT TCCAAAGGGG GAGATATCAT GCTCTGGAAT
TTTGGCATCA AGGACAAACC CACCTTCATC AAAGGGGCAG CCTGGCATCC TCGCTACAAC
CTCATTGTTG TGGGCCGATA CCCAGATCCT AATTTCAAAA GTTGTACCCC TTATGAATTG
AGGACGATCG ACGTGTTCGA TGGAAACTCA GGGAAGATGA TGTGTCAGCT CTATGACCCA
GAATCTTCTG GCATCAGTTC GCTTAATGAA TTCAATCCCA TGGGGGACAC GCTGGCCTCT
GCAATGGGTT ACCACATTCT CATCTGGAGC CAGGAGGAAG CCAGGACACG GAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A novel DDB2-ATM feedback loop regulates human cytomegalovirus replication
J. Virol. 88 (4), 2279-2290 (2014)
E X, Savidis G, Chin CR, Wang S, Lu S, Brass AL and Kowalik TF.


book

SUMOylation of damaged DNA-binding protein DDB2
Biochem. Biophys. Res. Commun. 438 (1), 26-31 (2013)
Tsuge M, Masuda Y, Kaneoka H, Kidani S, Miyake K and Iijima S.


book

DDB2: a novel regulator of NF-kappaB and breast tumor invasion
Cancer Res. 73 (16), 5040-5052 (2013)
Ennen M, Klotz R, Touche N, Pinel S, Barbieux C, Besancenot V, Brunner E, Thiebaut D, Jung AC, Ledrappier S, Domenjoud L, Abecassis J, Plenat F, Grandemange S and Becuwe P.


book

DDB2 suppresses epithelial-to-mesenchymal transition in colon cancer
Cancer Res. 73 (12), 3771-3782 (2013)
Roy N, Bommi PV, Bhat UG, Bhattacharjee S, Elangovan I, Li J, Patra KC, Kopanja D, Blunier A, Benya R, Bagchi S and Raychaudhuri P.


book

A novel cis-acting element from the 3'UTR of DNA damage-binding protein 2 mRNA links transcriptional and post-transcriptional regulation of gene expression
Nucleic Acids Res. 41 (11), 5692-5703 (2013)
Melanson BD, Cabrita MA, Bose R, Hamill JD, Pan E, Brochu C, Marcellus KA, Zhao TT, Holcik M and McKay BC.


book

DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1
Mol. Cell. Biol. 18 (1), 240-249 (1998)
Hayes S, Shiyanov P, Chen X and Raychaudhuri P.


book

Mutations specific to the xeroderma pigmentosum group E Ddb- phenotype
J. Biol. Chem. 271 (40), 24317-24320 (1996)
Nichols AF, Ong P and Linn S.


book

Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein
Genomics 29 (1), 62-69 (1995)
Dualan R, Brody T, Keeney S, Nichols AF, Admon A and Linn S.


book

Characterization of a human DNA damage binding protein implicated in xeroderma pigmentosum E
J. Biol. Chem. 268 (28), 21293-21300 (1993)
Keeney S, Chang GJ and Linn S.


book

Xeroderma Pigmentosum
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kraemer,K.H. and DiGiovanna,J.J.


 
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