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MMAA cDNA ORF clone, Homo sapiens (human)

Gene Symbol MMAA
Entrez Gene ID 166785
Full Name methylmalonic aciduria (cobalamin deficiency) cblA type
Synonyms cblA
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q31.21

Summary The protein encoded by this gene is involved in the translocation of cobalamin into the mitochondrion, where it is used in the final steps of adenosylcobalamin synthesis. Adenosylcobalamin is a coenzyme required for the activity of methylmalonyl-CoA mutase. Defects in this gene are a cause of methylmalonic aciduria. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

607481

Disorder Html: Methylmalonic aciduria, vitamin B12-responsive, 251100 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_172250 NP_758454 methylmalonic aciduria type A protein, mitochondrial precursor
XM_011531684 XP_011529986 methylmalonic aciduria type A protein, mitochondrial isoform X1
XM_011531685 XP_011529987 methylmalonic aciduria type A protein, mitochondrial isoform X1
XM_011531686 XP_011529988 methylmalonic aciduria type A protein, mitochondrial isoform X2



Homo sapiens (human) MMAA NP_758454.1
Pan troglodytes (chimpanzee) MMAA XP_526697.2
Canis lupus familiaris (dog) MMAA XP_539752.4
Bos taurus (cattle) MMAA NP_001093812.1
Mus musculus (house mouse) Mmaa NP_598584.2
Rattus norvegicus (Norway rat) Mmaa NP_001099644.1
Gallus gallus (chicken) MMAA XP_420429.1
Danio rerio (zebrafish) mmaa NP_001098582.1
Caenorhabditis elegans mmaa-1 NP_001022302.1
Xenopus (Silurana) tropicalis (western clawed frog) mmaa XP_002938979.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MMAA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMAA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26306
NM_172250 Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26306
XM_011531684 PREDICTED: Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26306
XM_011531685 PREDICTED: Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu77338 XM_011531686 PREDICTED: Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) NM_172250 , XM_011531684 , XM_011531685
Accession Version NM_172250.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product methylmalonic aciduria type A protein, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126662.1 and AC093864.3. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2008 this sequence version replaced gi:26892294. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
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241
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_758454.1
CDS86..1342
Misc Feature(1)38..40(+)
Misc Feature(2)341..1330(+)
Misc Feature(3)518..961(+)
Misc Feature(4)533..559(+)
Exon (1)1..20
Gene:MMAA
Gene Synonym:
Exon (2)21..524
Gene:MMAA
Gene Synonym:
Exon (3)525..647
Gene:MMAA
Gene Synonym:
Exon (4)648..818
Gene:MMAA
Gene Synonym:
Exon (5)819..904
Gene:MMAA
Gene Synonym:
Exon (6)905..1054
Gene:MMAA
Gene Synonym:
Exon (7)1055..5943
Gene:MMAA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_172250
Organism Homo sapiens (human)
Definition Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_172250

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) NM_172250 , XM_011531684 , XM_011531685
Accession Version XM_011531684.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529986.1
CDS142..1398
Misc Feature(1)397..1386(+)
Misc Feature(2)574..1017(+)
Misc Feature(3)589..615(+)
Translation

Target ORF information:

RefSeq Version XM_011531684
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531684

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26306
Clone ID Related Accession (Same CDS sequence) NM_172250 , XM_011531684 , XM_011531685
Accession Version XM_011531685.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529987.1
CDS151..1407
Misc Feature(1)406..1395(+)
Misc Feature(2)583..1026(+)
Misc Feature(3)598..624(+)
Translation

Target ORF information:

RefSeq Version XM_011531685
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531685

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCCATGC TGCTACCACA TCCTCACCAG CATTTCCTAA AAGGCCTTTT AAGAGCACCT 
TTCCGATGTT ACCACTTCAT CTTTCACTCA AGTACTCATC TCGGATCAGG AATCCCATGT
GCTCAGCCGT TTAATTCTCT TGGACTCCAT TGTACAAAGT GGATGCTGCT GTCAGATGGC
TTAAAGAGAA AATTATGTGT ACAAACAACC TTAAAGGACC ACACAGAAGG ACTTTCTGAT
AAAGAGCAAA GATTTGTGGA TAAACTTTAT ACTGGTTTAA TCCAAGGGCA AAGGGCCTGT
TTAGCAGAGG CCATAACTCT TGTAGAATCA ACTCACAGCA GGAAAAAGGA GTTAGCCCAG
GTGCTTCTTC AGAAAGTATT ACTTTACCAC AGAGAACAAG AACAATCAAA TAAAGGAAAA
CCACTAGCAT TTCGAGTAGG ATTGTCTGGG CCCCCTGGTG CTGGAAAATC AACATTTATA
GAATATTTTG GAAAAATGCT TACTGAGAGA GGGCACAAAT TATCTGTGCT AGCTGTGGAC
CCTTCTTCTT GTACTAGTGG TGGATCACTC TTAGGTGATA AAACCCGAAT GACTGAGTTA
TCAAGAGATA TGAATGCATA CATCAGGCCA TCTCCTACTA GAGGAACTTT AGGAGGCGTG
ACAAGGACCA CAAATGAAGC TATTCTGTTG TGTGAAGGAG CGGGATATGA CATAATTCTT
ATTGAAACCG TTGGTGTGGG TCAGTCGGAG TTTGCTGTTG CTGACATGGT TGACATGTTT
GTTTTACTAC TGCCACCAGC AGGAGGAGAT GAGCTGCAGG GTATCAAAAG GGGTATAATC
GAGATGGCAG ATCTGGTAGC TGTAACTAAA TCTGATGGAG ACTTGATTGT GCCAGCTCGA
AGGATACAAG CGGAATATGT GAGTGCACTG AAATTACTCC GCAAACGTTC ACAAGTCTGG
AAACCAAAGG TAATTCGTAT TTCTGCCCGA AGTGGAGAGG GGATCTCTGA AATGTGGGAT
AAAATGAAAG ATTTCCAGGA CCTAATGCTT GCCAGTGGGG AGCTGACTGC CAAACGACGG
AAGCAACAGA AAGTTTGGAT GTGGAATCTC ATTCAGGAAA GTGTGTTAGA GCATTTCAGG
ACCCACCCCA CAGTCCGGGA ACAGATTCCA CTTCTGGAAC AAAAGGTTCT CATTGGGGCC
CTGTCCCCAG GACTAGCAGC AGACTTCTTG TTAAAAGCTT TTAAAAGCAG AGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77338
Clone ID Related Accession (Same CDS sequence) XM_011531686
Accession Version XM_011531686.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 762bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methylmalonic aciduria type A protein, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTTACTG AGAGAGGGCA CAAATTATCT GTGCTAGCTG TGGACCCTTC TTCTTGTACT 
AGTGGTGGAT CACTCTTAGG TGATAAAACC CGAATGACTG AGTTATCAAG AGATATGAAT
GCATACATCA GGCCATCTCC TACTAGAGGA ACTTTAGGAG GCGTGACAAG GACCACAAAT
GAAGCTATTC TGTTGTGTGA AGGAGCGGGA TATGACATAA TTCTTATTGA AACCGTTGGT
GTGGGTCAGT CGGAGTTTGC TGTTGCTGAC ATGGTTGACA TGTTTGTTTT ACTACTGCCA
CCAGCAGGAG GAGATGAGCT GCAGGGTATC AAAAGGGGTA TAATCGAGAT GGCAGATCTG
GTAGCTGTAA CTAAATCTGA TGGAGACTTG ATTGTGCCAG CTCGAAGGAT ACAAGCGGAA
TATGTGAGTG CACTGAAATT ACTCCGCAAA CGTTCACAAG TCTGGAAACC AAAGGTAATT
CGTATTTCTG CCCGAAGTGG AGAGGGGATC TCTGAAATGT GGGATAAAAT GAAAGATTTC
CAGGACCTAA TGCTTGCCAG TGGGGAGCTG ACTGCCAAAC GACGGAAGCA ACAGAAAGTT
TGGATGTGGA ATCTCATTCA GGAAAGTGTG TTAGAGCATT TCAGGACCCA CCCCACAGTC
CGGGAACAGA TTCCACTTCT GGAACAAAAG GTTCTCATTG GGGCCCTGTC CCCAGGACTA
GCAGCAGACT TCTTGTTAAA AGCTTTTAAA AGCAGAGACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529988.1
CDS314..1075
Misc Feature(1)314..1072(+)
Translation

Target ORF information:

RefSeq Version XM_011531686
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531686

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTTACTG AGAGAGGGCA CAAATTATCT GTGCTAGCTG TGGACCCTTC TTCTTGTACT 
AGTGGTGGAT CACTCTTAGG TGATAAAACC CGAATGACTG AGTTATCAAG AGATATGAAT
GCATACATCA GGCCATCTCC TACTAGAGGA ACTTTAGGAG GCGTGACAAG GACCACAAAT
GAAGCTATTC TGTTGTGTGA AGGAGCGGGA TATGACATAA TTCTTATTGA AACCGTTGGT
GTGGGTCAGT CGGAGTTTGC TGTTGCTGAC ATGGTTGACA TGTTTGTTTT ACTACTGCCA
CCAGCAGGAG GAGATGAGCT GCAGGGTATC AAAAGGGGTA TAATCGAGAT GGCAGATCTG
GTAGCTGTAA CTAAATCTGA TGGAGACTTG ATTGTGCCAG CTCGAAGGAT ACAAGCGGAA
TATGTGAGTG CACTGAAATT ACTCCGCAAA CGTTCACAAG TCTGGAAACC AAAGGTAATT
CGTATTTCTG CCCGAAGTGG AGAGGGGATC TCTGAAATGT GGGATAAAAT GAAAGATTTC
CAGGACCTAA TGCTTGCCAG TGGGGAGCTG ACTGCCAAAC GACGGAAGCA ACAGAAAGTT
TGGATGTGGA ATCTCATTCA GGAAAGTGTG TTAGAGCATT TCAGGACCCA CCCCACAGTC
CGGGAACAGA TTCCACTTCT GGAACAAAAG GTTCTCATTG GGGCCCTGTC CCCAGGACTA
GCAGCAGACT TCTTGTTAAA AGCTTTTAAA AGCAGAGACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria
Mol. Genet. Metab. 107 (3), 363-367 (2012)
Dempsey-Nunez L, Illson ML, Kent J, Huang Q, Brebner A, Watkins D, Gilfix BM, Wittwer CT and Rosenblatt DS.


book

Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein
Biochem. Biophys. Res. Commun. 404 (1), 443-447 (2011)
Takahashi-Iniguez T, Garcia-Arellano H, Trujillo-Roldan MA and Flores ME.


book

Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation
J. Biol. Chem. 285 (49), 38204-38213 (2010)
Froese DS, Kochan G, Muniz JR, Wu X, Gileadi C, Ugochukwu E, Krysztofinska E, Gravel RA, Oppermann U and Yue WW.


book

Spondylocostal dysostosis associated with methylmalonic aciduria
Genet Test Mol Biomarkers 13 (2), 181-183 (2009)
Honjo RS, Casella EB, Vieira MA, Bertola DR, Albano LM, Oliveira LA, Nomachi S, Hanai J, Benoist JF, Ellard S, Young E and Kim CA.


book

Mutations in the MMAA gene in patients with the cblA disorder of vitamin B12 metabolism
Hum. Mutat. 24 (6), 509-516 (2004)
Lerner-Ellis JP, Dobson CM, Wai T, Watkins D, Tirone JC, Leclerc D, Dore C, Lepage P, Gravel RA and Rosenblatt DS.


book

Mutation analysis of the MMAA and MMAB genes in Japanese patients with vitamin B(12)-responsive methylmalonic acidemia: identification of a prevalent MMAA mutation
Mol. Genet. Metab. 82 (4), 329-333 (2004)
Yang X, Sakamoto O, Matsubara Y, Kure S, Suzuki Y, Aoki Y, Suzuki Y, Sakura N, Takayanagi M, Iinuma K and Ohura T.


book

Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements
Proc. Natl. Acad. Sci. U.S.A. 99 (24), 15554-15559 (2002)
Dobson CM, Wai T, Leclerc D, Wilson A, Wu X, Dore C, Hudson T, Rosenblatt DS and Gravel RA.


book

Methylmalonic Acidemia
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Manoli,I. and Venditti,C.P.


book

Dystonia Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Klein,C., Marras,C. and Munchau,A.


book

The Organic Acidemias: An Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Seashore,M.R.


 
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