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COCH cochlin [Homo sapiens (human)]

Gene Symbol COCH
Entrez Gene ID 1690
Full Name cochlin
Synonyms COCH-5B2, COCH5B2, DFNA9
General protein information
Preferred Names
cochlin
Names
cochlin
coagulation factor C homolog, cochlin (Limulus polyphemus)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q11.2-q13

Summary The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008]. lac of sum
Disorder MIM:

603196

Disorder Html: Deafness, autosomal dominant 9, 601369 (3)





The following COCH gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COCH gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27128 NM_001135058 Homo sapiens cochlin (COCH), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu27128 NM_004086 Homo sapiens cochlin (COCH), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu27128 XM_011536539 PREDICTED: Homo sapiens cochlin (COCH), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu27128
Accession Version NM_001135058.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cochlin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW629100.1, AY358900.1 and AF006740.1. Summary: The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008]. Transcript Variant: This variant (1) represents the longer variant. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001128530.1
CDS405..2057
Misc Feature(1)327..329(+)
Misc Feature(2)492..740(+)
Misc Feature(3)894..1388(+)
Misc Feature(4)915..1229(+)
Misc Feature(5)921..1130(+)
Misc Feature(6)1500..1985(+)
Misc Feature(7)1521..1829(+)
Misc Feature(8)1527..1736(+)
Misc Feature(9)1617..1712(+)
Exon (1)1..438
Gene:COCH
Gene Synonym:
Exon (2)439..486
Gene:COCH
Gene Synonym:
Exon (3)487..643
Gene:COCH
Gene Synonym:
Exon (4)644..777
Gene:COCH
Gene Synonym:
Exon (5)778..840
Gene:COCH
Gene Synonym:
Exon (6)841..885
Gene:COCH
Gene Synonym:
Exon (7)886..1033
Gene:COCH
Gene Synonym:
Exon (8)1034..1137
Gene:COCH
Gene Synonym:
Exon (9)1138..1364
Gene:COCH
Gene Synonym:
Exon (10)1365..1881
Gene:COCH
Gene Synonym:
Exon (11)1882..2882
Gene:COCH
Gene Synonym:
Translation
Position Chain Variation Link
31 31 a, g dbSNP:534136766
77 77 a, g dbSNP:559013815
82 82 c, t dbSNP:577631884
98 98 c, t dbSNP:540991911
100 100 a, g dbSNP:781390066
156 156 c, t dbSNP:748429961
197 197 a, g dbSNP:756345772
210 210 g, t dbSNP:28400022
259 259 a, g dbSNP:555903096
261 261 a, g dbSNP:541609857
274 274 a, t dbSNP:141450555
287 287 c, t dbSNP:771415523
294 294 c, t dbSNP:530652156
299 299 a, g dbSNP:11555426
308 308 -, g dbSNP:34495891
312 312 a, g dbSNP:564012553
316 316 c, t dbSNP:771771730
318 318 c, g dbSNP:775266701
326 326 c, g dbSNP:528387564
331 331 a, g dbSNP:78284050
355 355 c, g dbSNP:753640775
359 359 -, c dbSNP:561171408
362 362 a, g, t dbSNP:374605538
369 369 c, t dbSNP:751621900
371 371 c, t dbSNP:755076761
376 376 a, t dbSNP:781298231
378 378 c, t dbSNP:748340001
381 381 c, g, t dbSNP:565782596
394 394 a, t dbSNP:536336458
395 395 a, g dbSNP:200113981
408 408 a, t dbSNP:774750753
411 411 c, g dbSNP:544874880
424 424 c, t dbSNP:201427920
426 426 a, g dbSNP:200935305
429 429 c, g dbSNP:760397052
432 432 a, g dbSNP:763855435
435 435 c, t dbSNP:776423642
439 439 -, gt dbSNP:770529357
447 447 -, ctg dbSNP:774012347
449 449 g, t dbSNP:375459510
455 455 a, g dbSNP:368400670
459 459 a, c dbSNP:773128988
460 460 c, t dbSNP:200522736
463 463 a, g dbSNP:762867370
464 464 a, g dbSNP:766320448
467 467 c, g dbSNP:752704972
472 472 a, g dbSNP:576712224
477 477 a, c, g dbSNP:371802597
484 484 a, c dbSNP:113782841
486 486 a, g dbSNP:753980212
491 491 c, t dbSNP:774290415
496 496 a, c dbSNP:760735679
500 500 c, t dbSNP:759563279
505 505 a, g dbSNP:764050128
506 506 c, t dbSNP:754016575
512 512 c, t dbSNP:372237012
516 516 a, g dbSNP:765508608
521 521 a, g, t dbSNP:750779588
522 522 a, g, t dbSNP:143098658
527 527 c, g dbSNP:755579907
530 530 a, c, g dbSNP:146115619
545 545 c, t dbSNP:199674735
549 549 c, t dbSNP:777432281
555 555 c, t dbSNP:28938175
557 557 a, c dbSNP:573330522
559 559 a, g dbSNP:770686759
560 560 a, g dbSNP:774167584
562 562 c, g dbSNP:759387745
578 578 -, t dbSNP:766938007
581 581 a, c dbSNP:772104254
591 591 -, g dbSNP:775128463
592 592 c, g dbSNP:776514642
596 596 c, t dbSNP:761955986
597 597 a, g dbSNP:148102471
598 598 g, t dbSNP:765509637
601 601 c, g, t dbSNP:121908927
602 602 a, g, t dbSNP:28400029
606 606 g, t dbSNP:752031300
611 611 c, t dbSNP:755385664
614 614 a, t dbSNP:560972920
616 616 c, t dbSNP:781725849
617 617 a, g dbSNP:200899172
627 627 a, g dbSNP:755669391
628 628 a, g dbSNP:369827155
629 629 a, g dbSNP:748904202
635 635 a, c, t dbSNP:756970715
641 641 c, t dbSNP:764134844
650 650 a, g dbSNP:779756178
652 652 c, t dbSNP:746962272
654 654 a, g dbSNP:768776389
659 659 a, c dbSNP:773371394
661 661 c, t dbSNP:749386539
667 667 a, g, t dbSNP:121908928
670 670 a, c dbSNP:149903169
675 675 c, g, t dbSNP:540895576
676 676 a, g dbSNP:188283330
686 686 a, c dbSNP:775781203
690 690 a, c, t dbSNP:761156534
692 692 c, t dbSNP:144952367
696 696 c, t dbSNP:756790858
697 697 a, g dbSNP:764704351
704 704 c, t dbSNP:750061309
705 705 -, ta dbSNP:754218343
707 707 c, t dbSNP:758053398
725 725 c, t dbSNP:779886624
730 730 a, t dbSNP:121908930
741 741 a, g dbSNP:751417706
753 753 c, t dbSNP:121908929
759 759 a, g dbSNP:121908931
778 778 a, g dbSNP:756069066
781 781 a, g dbSNP:779033796
782 782 c, t dbSNP:148599620
792 792 a, g dbSNP:772269857
807 807 a, g dbSNP:28400035
808 808 a, g dbSNP:747323881
810 810 -, caag dbSNP:758761384
828 828 c, t dbSNP:142073417
832 832 c, t dbSNP:777049577
833 833 a, g dbSNP:147841606
846 846 a, c dbSNP:755252656
847 847 a, c, g dbSNP:543399762
853 853 a, c dbSNP:770299353
854 854 -, g dbSNP:34622961
863 863 c, t dbSNP:773617215
864 864 a, g, t dbSNP:749858367
866 866 a, g dbSNP:774069829
878 878 a, c dbSNP:759246142
900 900 a, g dbSNP:771810191
904 904 c, t dbSNP:775303808
914 914 g, t dbSNP:760423119
922 922 a, g dbSNP:763763889
923 923 c, t dbSNP:771252412
931 931 c, t dbSNP:541167867
938 938 a, g dbSNP:765164378
940 940 a, g dbSNP:751600633
942 942 c, t dbSNP:559481815
943 943 a, g dbSNP:369882771
951 951 a, t dbSNP:752834753
959 959 a, g dbSNP:146395267
970 970 a, g dbSNP:756422562
975 975 a, g dbSNP:141421714
979 979 c, t dbSNP:754241461
986 986 g, t dbSNP:757768757
987 987 c, t dbSNP:779593424
992 992 a, g dbSNP:745321921
996 996 a, g dbSNP:771685804
996 996 -, g dbSNP:755973091
1014 1014 a, g dbSNP:200549732
1019 1019 c, t dbSNP:548387738
1033 1033 a, g dbSNP:768368801
1035 1035 g, t dbSNP:746532969
1040 1040 c, t dbSNP:768156505
1059 1059 c, t dbSNP:780921835
1072 1072 c, t dbSNP:747849307
1073 1073 a, g dbSNP:370307569
1083 1083 a, g dbSNP:773061827
1085 1085 c, t dbSNP:147017172
1086 1086 a, g dbSNP:751022116
1096 1096 c, t dbSNP:536511561
1098 1098 a, g dbSNP:201294938
1107 1107 a, g dbSNP:760837124
1108 1108 c, t dbSNP:764171622
1109 1109 a, g dbSNP:372549728
1121 1121 a, g dbSNP:200392534
1133 1133 c, t dbSNP:765692468
1135 1135 c, t dbSNP:750832640
1142 1142 a, g dbSNP:778069510
1144 1144 c, g dbSNP:749516210
1148 1148 a, g dbSNP:370194438
1153 1153 a, g dbSNP:774715234
1164 1164 a, c dbSNP:759845948
1175 1175 a, g dbSNP:772516874
1190 1190 a, g dbSNP:375612298
1198 1198 a, g dbSNP:761209524
1200 1200 a, c dbSNP:763588874
1209 1209 g, t dbSNP:531508748
1215 1215 a, g dbSNP:761471633
1216 1216 -, g dbSNP:762204431
1216 1216 g, t dbSNP:77074957
1218 1218 a, g dbSNP:186875564
1231 1231 g, t dbSNP:750185209
1245 1245 a, g dbSNP:28362775
1250 1250 c, t dbSNP:368609319
1267 1267 c, t dbSNP:751540613
1282 1282 a, g dbSNP:755026676
1296 1296 a, g dbSNP:550268175
1300 1300 c, t dbSNP:777855403
1302 1302 g, t dbSNP:749336302
1303 1303 a, c dbSNP:757456469
1304 1304 c, t dbSNP:779021334
1313 1313 a, c, g dbSNP:150207791
1315 1315 c, t dbSNP:775897050
1316 1316 c, t dbSNP:558120815
1322 1322 g, t dbSNP:138805494
1331 1331 a, g dbSNP:149342451
1341 1341 c, t dbSNP:761251617
1355 1355 c, t dbSNP:764852134
1361 1361 c, t dbSNP:772709865
1364 1364 a, g dbSNP:762762265
1373 1373 c, t dbSNP:769102282
1374 1374 c, t dbSNP:776982710
1375 1375 a, g dbSNP:748731866
1385 1385 c, t dbSNP:769184887
1388 1388 c, t dbSNP:772839507
1391 1391 c, t dbSNP:762639586
1393 1393 c, t dbSNP:766138580
1396 1396 a, t dbSNP:774252448
1397 1397 a, c dbSNP:759421873
1399 1399 a, g dbSNP:376820760
1402 1402 a, g, t dbSNP:752542741
1408 1408 a, g dbSNP:765216094
1413 1413 c, t dbSNP:370525728
1415 1415 c, t dbSNP:758549167
1416 1416 c, g dbSNP:780286623
1429 1429 a, g dbSNP:751829593
1430 1430 c, t dbSNP:28362777
1431 1431 a, g dbSNP:544358450
1446 1446 c, g dbSNP:748591988
1450 1450 -, agct dbSNP:751552396
1451 1451 g, t dbSNP:770350333
1454 1454 a, g dbSNP:138248389
1459 1459 c, g dbSNP:1045644
1487 1487 c, g, t dbSNP:371011287
1492 1492 a, g dbSNP:774121028
1495 1495 a, c dbSNP:759299023
1496 1496 -, c dbSNP:36109457
1502 1502 a, g dbSNP:771756402
1507 1507 a, c, t dbSNP:375542487
1509 1509 c, g dbSNP:764096356
1519 1519 -, t dbSNP:755106768
1526 1526 a, c dbSNP:755142161
1532 1532 c, t dbSNP:146166304
1536 1536 a, g dbSNP:762998217
1537 1537 c, t dbSNP:370382942
1539 1539 a, g dbSNP:267603975
1540 1540 -, agat dbSNP:777627665
1544 1544 a, c, t dbSNP:577912568
1547 1547 a, c dbSNP:781355952
1550 1550 c, t dbSNP:753113360
1554 1554 c, t dbSNP:756541797
1555 1555 a, g dbSNP:545418366
1556 1556 c, t dbSNP:140108742
1560 1560 a, g dbSNP:770530713
1564 1564 a, g, t dbSNP:571941613
1571 1571 c, t dbSNP:77370101
1588 1588 a, g dbSNP:200904881
1592 1592 c, t dbSNP:771767136
1601 1601 a, c dbSNP:775079663
1603 1603 c, t dbSNP:760549722
1604 1604 a, g dbSNP:150529026
1605 1605 a, g dbSNP:776519637
1608 1608 a, g dbSNP:28362778
1609 1609 c, t dbSNP:766435124
1618 1618 a, c dbSNP:751654014
1621 1621 c, t dbSNP:748478920
1625 1625 c, t dbSNP:199608109
1643 1643 c, t dbSNP:767814589
1650 1650 c, t dbSNP:367884240
1651 1651 a, g dbSNP:772383772
1654 1654 c, t dbSNP:756473964
1655 1655 a, g dbSNP:549957619
1673 1673 c, g, t dbSNP:35353967
1674 1674 g, t dbSNP:61759484
1684 1684 a, g dbSNP:745390437
1690 1690 a, g dbSNP:757948966
1714 1714 g, t dbSNP:779778621
1717 1717 a, g dbSNP:746680829
1719 1719 a, t dbSNP:768435123
1720 1720 a, c dbSNP:776467069
1722 1722 a, g dbSNP:748019236
1723 1723 c, t dbSNP:769725783
1728 1728 c, g dbSNP:774252846
1731 1731 a, g dbSNP:759679894
1734 1734 a, g dbSNP:371248806
1752 1752 a, g dbSNP:139503327
1761 1761 a, g dbSNP:760876932
1762 1762 a, c dbSNP:764544231
1764 1764 g, t dbSNP:754271185
1768 1768 c, g dbSNP:757774488
1775 1775 a, g dbSNP:765878055
1780 1780 a, g dbSNP:371099832
1782 1782 c, t dbSNP:749854026
1840 1840 a, t dbSNP:757904271
1852 1852 a, g dbSNP:779496405
1855 1855 a, g dbSNP:746659336
1864 1864 c, g dbSNP:754636399
1887 1887 a, g dbSNP:770789480
1888 1888 c, g dbSNP:780116790
1889 1889 -, a dbSNP:779782495
1912 1912 g, t dbSNP:200252533
1915 1915 c, g dbSNP:768952355
1917 1917 a, c dbSNP:201578645
1919 1919 c, t dbSNP:762167606
1925 1925 a, t dbSNP:564563172
1939 1939 c, t dbSNP:121908934
1945 1945 c, t dbSNP:763449449
1947 1947 a, t dbSNP:766825368
1951 1951 a, c dbSNP:751018920
1952 1952 a, g dbSNP:747088615
1953 1953 a, g dbSNP:759022387
1957 1957 a, g dbSNP:17097468
1974 1974 a, c dbSNP:752337401
1975 1975 c, t dbSNP:755826383
1977 1977 -, agag dbSNP:746449866
1978 1978 g, t dbSNP:147643069
1986 1986 a, c dbSNP:753583403
1995 1995 c, g dbSNP:149072811
1998 1998 a, c, t dbSNP:1801963
2012 2012 c, t dbSNP:745850559
2019 2019 a, g dbSNP:768825582
2022 2022 c, g dbSNP:781479122
2023 2023 c, g dbSNP:748322615
2027 2027 c, t dbSNP:769911394
2029 2029 a, g, t dbSNP:121908932
2032 2032 a, g dbSNP:143142304
2038 2038 a, t dbSNP:763246563
2040 2040 c, t dbSNP:771372583
2051 2051 c, g dbSNP:774804924
2052 2052 a, c dbSNP:747736722
2053 2053 -, aat dbSNP:768219316
2060 2060 -, t dbSNP:776347723
2080 2080 -, g dbSNP:762396837
2083 2083 -, t dbSNP:770473394
2083 2083 a, g dbSNP:760097850
2084 2084 a, t dbSNP:13122
2088 2088 g, t dbSNP:774998807
2090 2090 c, g dbSNP:760310994
2096 2096 g, t dbSNP:555882283
2100 2100 -, ca dbSNP:773680146
2111 2111 c, t dbSNP:575946401
2114 2114 a, t dbSNP:550166243
2171 2171 c, g, t dbSNP:375692052
2204 2204 c, t dbSNP:532927106
2232 2232 -, t dbSNP:575558071
2241 2241 c, t dbSNP:368151461
2246 2246 c, t dbSNP:551552278
2270 2270 a, c dbSNP:761019416
2294 2294 c, g dbSNP:190142757
2302 2302 a, g dbSNP:45624239
2309 2309 c, t dbSNP:372169208
2329 2329 a, g dbSNP:375156330
2342 2342 c, g dbSNP:369400127
2396 2396 c, t dbSNP:187461561
2414 2414 c, t dbSNP:542135133
2415 2415 a, g dbSNP:774390888
2454 2454 -, aa dbSNP:546373097
2464 2464 c, t dbSNP:7152503
2477 2477 a, g dbSNP:535801007
2541 2541 -, aat dbSNP:10610193
2545 2545 -, ata dbSNP:61035395
2570 2570 a, t dbSNP:557399889
2589 2589 g, t dbSNP:748054345
2620 2620 a, t dbSNP:575682842
2633 2633 g, t dbSNP:768839032
2640 2640 c, t dbSNP:77851565
2647 2647 a, g dbSNP:371554692
2657 2657 a, g dbSNP:374727844
2666 2666 -, caggattgc dbSNP:535642374
2669 2669 g, t dbSNP:541766600
2682 2682 c, t dbSNP:143467211
2695 2695 g, t dbSNP:573389803
2723 2723 c, t dbSNP:56229109
2768 2768 -, gtaaa dbSNP:10562264
2770 2770 -, aaagt dbSNP:386574592
2770 2770 -, acttt dbSNP:200244885
2771 2771 -, aagta dbSNP:72262576
2843 2843 c, t dbSNP:562000407
2856 2856 a, g dbSNP:529554825
2871 2871 a, g dbSNP:543797568

Target ORF information:

RefSeq Version NM_001135058
Organism Homo sapiens (human)
Definition Homo sapiens cochlin (COCH), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135058

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27128
Accession Version NM_004086.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cochlin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW629100.1 and AF006740.1. This sequence is a reference standard in the RefSeqGene project. On Sep 19, 2008 this sequence version replaced gi:4758021. Summary: The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF006740.1, BC000640.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_004077.1
CDS81..1733
Misc Feature(1)168..416(+)
Misc Feature(2)570..1064(+)
Misc Feature(3)591..905(+)
Misc Feature(4)597..806(+)
Misc Feature(5)1176..1661(+)
Misc Feature(6)1197..1505(+)
Misc Feature(7)1203..1412(+)
Misc Feature(8)1293..1388(+)
Exon (1)1..57
Gene:COCH
Gene Synonym:
Exon (2)58..114
Gene:COCH
Gene Synonym:
Exon (3)115..162
Gene:COCH
Gene Synonym:
Exon (4)163..319
Gene:COCH
Gene Synonym:
Exon (5)320..453
Gene:COCH
Gene Synonym:
Exon (6)454..516
Gene:COCH
Gene Synonym:
Exon (7)517..561
Gene:COCH
Gene Synonym:
Exon (8)562..709
Gene:COCH
Gene Synonym:
Exon (9)710..813
Gene:COCH
Gene Synonym:
Exon (10)814..1040
Gene:COCH
Gene Synonym:
Exon (11)1041..1557
Gene:COCH
Gene Synonym:
Exon (12)1558..2558
Gene:COCH
Gene Synonym:
Translation
Position Chain Variation Link
31 31 a, g dbSNP:534136766
70 70 a, t dbSNP:536336458
71 71 a, g dbSNP:200113981
84 84 a, t dbSNP:774750753
87 87 c, g dbSNP:544874880
100 100 c, t dbSNP:201427920
102 102 a, g dbSNP:200935305
105 105 c, g dbSNP:760397052
108 108 a, g dbSNP:763855435
111 111 c, t dbSNP:776423642
115 115 -, gt dbSNP:770529357
123 123 -, ctg dbSNP:774012347
125 125 g, t dbSNP:375459510
131 131 a, g dbSNP:368400670
135 135 a, c dbSNP:773128988
136 136 c, t dbSNP:200522736
139 139 a, g dbSNP:762867370
140 140 a, g dbSNP:766320448
143 143 c, g dbSNP:752704972
148 148 a, g dbSNP:576712224
153 153 a, c, g dbSNP:371802597
160 160 a, c dbSNP:113782841
162 162 a, g dbSNP:753980212
167 167 c, t dbSNP:774290415
172 172 a, c dbSNP:760735679
176 176 c, t dbSNP:759563279
181 181 a, g dbSNP:764050128
182 182 c, t dbSNP:754016575
188 188 c, t dbSNP:372237012
192 192 a, g dbSNP:765508608
197 197 a, g, t dbSNP:750779588
198 198 a, g, t dbSNP:143098658
203 203 c, g dbSNP:755579907
206 206 a, c, g dbSNP:146115619
221 221 c, t dbSNP:199674735
225 225 c, t dbSNP:777432281
231 231 c, t dbSNP:28938175
233 233 a, c dbSNP:573330522
235 235 a, g dbSNP:770686759
236 236 a, g dbSNP:774167584
238 238 c, g dbSNP:759387745
254 254 -, t dbSNP:766938007
257 257 a, c dbSNP:772104254
267 267 -, g dbSNP:775128463
268 268 c, g dbSNP:776514642
272 272 c, t dbSNP:761955986
273 273 a, g dbSNP:148102471
274 274 g, t dbSNP:765509637
277 277 c, g, t dbSNP:121908927
278 278 a, g, t dbSNP:28400029
282 282 g, t dbSNP:752031300
287 287 c, t dbSNP:755385664
290 290 a, t dbSNP:560972920
292 292 c, t dbSNP:781725849
293 293 a, g dbSNP:200899172
303 303 a, g dbSNP:755669391
304 304 a, g dbSNP:369827155
305 305 a, g dbSNP:748904202
311 311 a, c, t dbSNP:756970715
317 317 c, t dbSNP:764134844
326 326 a, g dbSNP:779756178
328 328 c, t dbSNP:746962272
330 330 a, g dbSNP:768776389
335 335 a, c dbSNP:773371394
337 337 c, t dbSNP:749386539
343 343 a, g, t dbSNP:121908928
346 346 a, c dbSNP:149903169
351 351 c, g, t dbSNP:540895576
352 352 a, g dbSNP:188283330
362 362 a, c dbSNP:775781203
366 366 a, c, t dbSNP:761156534
368 368 c, t dbSNP:144952367
372 372 c, t dbSNP:756790858
373 373 a, g dbSNP:764704351
380 380 c, t dbSNP:750061309
381 381 -, ta dbSNP:754218343
383 383 c, t dbSNP:758053398
401 401 c, t dbSNP:779886624
406 406 a, t dbSNP:121908930
417 417 a, g dbSNP:751417706
429 429 c, t dbSNP:121908929
435 435 a, g dbSNP:121908931
454 454 a, g dbSNP:756069066
457 457 a, g dbSNP:779033796
458 458 c, t dbSNP:148599620
468 468 a, g dbSNP:772269857
483 483 a, g dbSNP:28400035
484 484 a, g dbSNP:747323881
486 486 -, caag dbSNP:758761384
504 504 c, t dbSNP:142073417
508 508 c, t dbSNP:777049577
509 509 a, g dbSNP:147841606
522 522 a, c dbSNP:755252656
523 523 a, c, g dbSNP:543399762
529 529 a, c dbSNP:770299353
530 530 -, g dbSNP:34622961
539 539 c, t dbSNP:773617215
540 540 a, g, t dbSNP:749858367
542 542 a, g dbSNP:774069829
554 554 a, c dbSNP:759246142
576 576 a, g dbSNP:771810191
580 580 c, t dbSNP:775303808
590 590 g, t dbSNP:760423119
598 598 a, g dbSNP:763763889
599 599 c, t dbSNP:771252412
607 607 c, t dbSNP:541167867
614 614 a, g dbSNP:765164378
616 616 a, g dbSNP:751600633
618 618 c, t dbSNP:559481815
619 619 a, g dbSNP:369882771
627 627 a, t dbSNP:752834753
635 635 a, g dbSNP:146395267
646 646 a, g dbSNP:756422562
651 651 a, g dbSNP:141421714
655 655 c, t dbSNP:754241461
662 662 g, t dbSNP:757768757
663 663 c, t dbSNP:779593424
668 668 a, g dbSNP:745321921
672 672 a, g dbSNP:771685804
672 672 -, g dbSNP:755973091
690 690 a, g dbSNP:200549732
695 695 c, t dbSNP:548387738
709 709 a, g dbSNP:768368801
711 711 g, t dbSNP:746532969
716 716 c, t dbSNP:768156505
735 735 c, t dbSNP:780921835
748 748 c, t dbSNP:747849307
749 749 a, g dbSNP:370307569
759 759 a, g dbSNP:773061827
761 761 c, t dbSNP:147017172
762 762 a, g dbSNP:751022116
772 772 c, t dbSNP:536511561
774 774 a, g dbSNP:201294938
783 783 a, g dbSNP:760837124
784 784 c, t dbSNP:764171622
785 785 a, g dbSNP:372549728
797 797 a, g dbSNP:200392534
809 809 c, t dbSNP:765692468
811 811 c, t dbSNP:750832640
818 818 a, g dbSNP:778069510
820 820 c, g dbSNP:749516210
824 824 a, g dbSNP:370194438
829 829 a, g dbSNP:774715234
840 840 a, c dbSNP:759845948
851 851 a, g dbSNP:772516874
866 866 a, g dbSNP:375612298
874 874 a, g dbSNP:761209524
876 876 a, c dbSNP:763588874
885 885 g, t dbSNP:531508748
891 891 a, g dbSNP:761471633
892 892 -, g dbSNP:762204431
892 892 g, t dbSNP:77074957
894 894 a, g dbSNP:186875564
907 907 g, t dbSNP:750185209
921 921 a, g dbSNP:28362775
926 926 c, t dbSNP:368609319
943 943 c, t dbSNP:751540613
958 958 a, g dbSNP:755026676
972 972 a, g dbSNP:550268175
976 976 c, t dbSNP:777855403
978 978 g, t dbSNP:749336302
979 979 a, c dbSNP:757456469
980 980 c, t dbSNP:779021334
989 989 a, c, g dbSNP:150207791
991 991 c, t dbSNP:775897050
992 992 c, t dbSNP:558120815
998 998 g, t dbSNP:138805494
1007 1007 a, g dbSNP:149342451
1017 1017 c, t dbSNP:761251617
1031 1031 c, t dbSNP:764852134
1037 1037 c, t dbSNP:772709865
1040 1040 a, g dbSNP:762762265
1049 1049 c, t dbSNP:769102282
1050 1050 c, t dbSNP:776982710
1051 1051 a, g dbSNP:748731866
1061 1061 c, t dbSNP:769184887
1064 1064 c, t dbSNP:772839507
1067 1067 c, t dbSNP:762639586
1069 1069 c, t dbSNP:766138580
1072 1072 a, t dbSNP:774252448
1073 1073 a, c dbSNP:759421873
1075 1075 a, g dbSNP:376820760
1078 1078 a, g, t dbSNP:752542741
1084 1084 a, g dbSNP:765216094
1089 1089 c, t dbSNP:370525728
1091 1091 c, t dbSNP:758549167
1092 1092 c, g dbSNP:780286623
1105 1105 a, g dbSNP:751829593
1106 1106 c, t dbSNP:28362777
1107 1107 a, g dbSNP:544358450
1122 1122 c, g dbSNP:748591988
1126 1126 -, agct dbSNP:751552396
1127 1127 g, t dbSNP:770350333
1130 1130 a, g dbSNP:138248389
1135 1135 c, g dbSNP:1045644
1163 1163 c, g, t dbSNP:371011287
1168 1168 a, g dbSNP:774121028
1171 1171 a, c dbSNP:759299023
1172 1172 -, c dbSNP:36109457
1178 1178 a, g dbSNP:771756402
1183 1183 a, c, t dbSNP:375542487
1185 1185 c, g dbSNP:764096356
1195 1195 -, t dbSNP:755106768
1202 1202 a, c dbSNP:755142161
1208 1208 c, t dbSNP:146166304
1212 1212 a, g dbSNP:762998217
1213 1213 c, t dbSNP:370382942
1215 1215 a, g dbSNP:267603975
1216 1216 -, agat dbSNP:777627665
1220 1220 a, c, t dbSNP:577912568
1223 1223 a, c dbSNP:781355952
1226 1226 c, t dbSNP:753113360
1230 1230 c, t dbSNP:756541797
1231 1231 a, g dbSNP:545418366
1232 1232 c, t dbSNP:140108742
1236 1236 a, g dbSNP:770530713
1240 1240 a, g, t dbSNP:571941613
1247 1247 c, t dbSNP:77370101
1264 1264 a, g dbSNP:200904881
1268 1268 c, t dbSNP:771767136
1277 1277 a, c dbSNP:775079663
1279 1279 c, t dbSNP:760549722
1280 1280 a, g dbSNP:150529026
1281 1281 a, g dbSNP:776519637
1284 1284 a, g dbSNP:28362778
1285 1285 c, t dbSNP:766435124
1294 1294 a, c dbSNP:751654014
1297 1297 c, t dbSNP:748478920
1301 1301 c, t dbSNP:199608109
1319 1319 c, t dbSNP:767814589
1326 1326 c, t dbSNP:367884240
1327 1327 a, g dbSNP:772383772
1330 1330 c, t dbSNP:756473964
1331 1331 a, g dbSNP:549957619
1349 1349 c, g, t dbSNP:35353967
1350 1350 g, t dbSNP:61759484
1360 1360 a, g dbSNP:745390437
1366 1366 a, g dbSNP:757948966
1390 1390 g, t dbSNP:779778621
1393 1393 a, g dbSNP:746680829
1395 1395 a, t dbSNP:768435123
1396 1396 a, c dbSNP:776467069
1398 1398 a, g dbSNP:748019236
1399 1399 c, t dbSNP:769725783
1404 1404 c, g dbSNP:774252846
1407 1407 a, g dbSNP:759679894
1410 1410 a, g dbSNP:371248806
1428 1428 a, g dbSNP:139503327
1437 1437 a, g dbSNP:760876932
1438 1438 a, c dbSNP:764544231
1440 1440 g, t dbSNP:754271185
1444 1444 c, g dbSNP:757774488
1451 1451 a, g dbSNP:765878055
1456 1456 a, g dbSNP:371099832
1458 1458 c, t dbSNP:749854026
1516 1516 a, t dbSNP:757904271
1528 1528 a, g dbSNP:779496405
1531 1531 a, g dbSNP:746659336
1540 1540 c, g dbSNP:754636399
1563 1563 a, g dbSNP:770789480
1564 1564 c, g dbSNP:780116790
1565 1565 -, a dbSNP:779782495
1588 1588 g, t dbSNP:200252533
1591 1591 c, g dbSNP:768952355
1593 1593 a, c dbSNP:201578645
1595 1595 c, t dbSNP:762167606
1601 1601 a, t dbSNP:564563172
1615 1615 c, t dbSNP:121908934
1621 1621 c, t dbSNP:763449449
1623 1623 a, t dbSNP:766825368
1627 1627 a, c dbSNP:751018920
1628 1628 a, g dbSNP:747088615
1629 1629 a, g dbSNP:759022387
1633 1633 a, g dbSNP:17097468
1650 1650 a, c dbSNP:752337401
1651 1651 c, t dbSNP:755826383
1653 1653 -, agag dbSNP:746449866
1654 1654 g, t dbSNP:147643069
1662 1662 a, c dbSNP:753583403
1671 1671 c, g dbSNP:149072811
1674 1674 a, c, t dbSNP:1801963
1688 1688 c, t dbSNP:745850559
1695 1695 a, g dbSNP:768825582
1698 1698 c, g dbSNP:781479122
1699 1699 c, g dbSNP:748322615
1703 1703 c, t dbSNP:769911394
1705 1705 a, g, t dbSNP:121908932
1708 1708 a, g dbSNP:143142304
1714 1714 a, t dbSNP:763246563
1716 1716 c, t dbSNP:771372583
1727 1727 c, g dbSNP:774804924
1728 1728 a, c dbSNP:747736722
1729 1729 -, aat dbSNP:768219316
1736 1736 -, t dbSNP:776347723
1756 1756 -, g dbSNP:762396837
1759 1759 -, t dbSNP:770473394
1759 1759 a, g dbSNP:760097850
1760 1760 a, t dbSNP:13122
1764 1764 g, t dbSNP:774998807
1766 1766 c, g dbSNP:760310994
1772 1772 g, t dbSNP:555882283
1776 1776 -, ca dbSNP:773680146
1787 1787 c, t dbSNP:575946401
1790 1790 a, t dbSNP:550166243
1847 1847 c, g, t dbSNP:375692052
1880 1880 c, t dbSNP:532927106
1908 1908 -, t dbSNP:575558071
1917 1917 c, t dbSNP:368151461
1922 1922 c, t dbSNP:551552278
1946 1946 a, c dbSNP:761019416
1970 1970 c, g dbSNP:190142757
1978 1978 a, g dbSNP:45624239
1985 1985 c, t dbSNP:372169208
2005 2005 a, g dbSNP:375156330
2018 2018 c, g dbSNP:369400127
2072 2072 c, t dbSNP:187461561
2090 2090 c, t dbSNP:542135133
2091 2091 a, g dbSNP:774390888
2130 2130 -, aa dbSNP:546373097
2140 2140 c, t dbSNP:7152503
2153 2153 a, g dbSNP:535801007
2217 2217 -, aat dbSNP:10610193
2221 2221 -, ata dbSNP:61035395
2246 2246 a, t dbSNP:557399889
2265 2265 g, t dbSNP:748054345
2296 2296 a, t dbSNP:575682842
2309 2309 g, t dbSNP:768839032
2316 2316 c, t dbSNP:77851565
2323 2323 a, g dbSNP:371554692
2333 2333 a, g dbSNP:374727844
2342 2342 -, caggattgc dbSNP:535642374
2345 2345 g, t dbSNP:541766600
2358 2358 c, t dbSNP:143467211
2371 2371 g, t dbSNP:573389803
2399 2399 c, t dbSNP:56229109
2444 2444 -, gtaaa dbSNP:10562264
2446 2446 -, aaagt dbSNP:386574592
2446 2446 -, acttt dbSNP:200244885
2447 2447 -, aagta dbSNP:72262576
2519 2519 c, t dbSNP:562000407
2532 2532 a, g dbSNP:529554825
2547 2547 a, g dbSNP:543797568

Target ORF information:

RefSeq Version NM_004086
Organism Homo sapiens (human)
Definition Homo sapiens cochlin (COCH), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004086

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27128
Accession Version XM_011536539.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cochlin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011534841.1
CDS55..1707
Misc Feature(1)142..390(+)
Misc Feature(2)544..1038(+)
Misc Feature(3)565..879(+)
Misc Feature(4)571..780(+)
Misc Feature(5)1150..1635(+)
Misc Feature(6)1171..1479(+)
Misc Feature(7)1177..1386(+)
Misc Feature(8)1267..1362(+)
Translation
Position Chain Variation Link
5 5 a, g dbSNP:534136766
44 44 a, t dbSNP:536336458
45 45 a, g dbSNP:200113981
58 58 a, t dbSNP:774750753
61 61 c, g dbSNP:544874880
74 74 c, t dbSNP:201427920
76 76 a, g dbSNP:200935305
79 79 c, g dbSNP:760397052
82 82 a, g dbSNP:763855435
85 85 c, t dbSNP:776423642
89 89 -, gt dbSNP:770529357
97 97 -, ctg dbSNP:774012347
99 99 g, t dbSNP:375459510
105 105 a, g dbSNP:368400670
109 109 a, c dbSNP:773128988
110 110 c, t dbSNP:200522736
113 113 a, g dbSNP:762867370
114 114 a, g dbSNP:766320448
117 117 c, g dbSNP:752704972
122 122 a, g dbSNP:576712224
127 127 a, c, g dbSNP:371802597
134 134 a, c dbSNP:113782841
136 136 a, g dbSNP:753980212
141 141 c, t dbSNP:774290415
146 146 a, c dbSNP:760735679
150 150 c, t dbSNP:759563279
155 155 a, g dbSNP:764050128
156 156 c, t dbSNP:754016575
162 162 c, t dbSNP:372237012
166 166 a, g dbSNP:765508608
171 171 a, g, t dbSNP:750779588
172 172 a, g, t dbSNP:143098658
177 177 c, g dbSNP:755579907
180 180 a, c, g dbSNP:146115619
195 195 c, t dbSNP:199674735
199 199 c, t dbSNP:777432281
205 205 c, t dbSNP:28938175
207 207 a, c dbSNP:573330522
209 209 a, g dbSNP:770686759
210 210 a, g dbSNP:774167584
212 212 c, g dbSNP:759387745
228 228 -, t dbSNP:766938007
231 231 a, c dbSNP:772104254
241 241 -, g dbSNP:775128463
242 242 c, g dbSNP:776514642
246 246 c, t dbSNP:761955986
247 247 a, g dbSNP:148102471
248 248 g, t dbSNP:765509637
251 251 c, g, t dbSNP:121908927
252 252 a, g, t dbSNP:28400029
256 256 g, t dbSNP:752031300
261 261 c, t dbSNP:755385664
264 264 a, t dbSNP:560972920
266 266 c, t dbSNP:781725849
267 267 a, g dbSNP:200899172
277 277 a, g dbSNP:755669391
278 278 a, g dbSNP:369827155
279 279 a, g dbSNP:748904202
285 285 a, c, t dbSNP:756970715
291 291 c, t dbSNP:764134844
300 300 a, g dbSNP:779756178
302 302 c, t dbSNP:746962272
304 304 a, g dbSNP:768776389
309 309 a, c dbSNP:773371394
311 311 c, t dbSNP:749386539
317 317 a, g, t dbSNP:121908928
320 320 a, c dbSNP:149903169
325 325 c, g, t dbSNP:540895576
326 326 a, g dbSNP:188283330
336 336 a, c dbSNP:775781203
340 340 a, c, t dbSNP:761156534
342 342 c, t dbSNP:144952367
346 346 c, t dbSNP:756790858
347 347 a, g dbSNP:764704351
354 354 c, t dbSNP:750061309
355 355 -, ta dbSNP:754218343
357 357 c, t dbSNP:758053398
375 375 c, t dbSNP:779886624
380 380 a, t dbSNP:121908930
391 391 a, g dbSNP:751417706
403 403 c, t dbSNP:121908929
409 409 a, g dbSNP:121908931
428 428 a, g dbSNP:756069066
431 431 a, g dbSNP:779033796
432 432 c, t dbSNP:148599620
442 442 a, g dbSNP:772269857
457 457 a, g dbSNP:28400035
458 458 a, g dbSNP:747323881
460 460 -, caag dbSNP:758761384
478 478 c, t dbSNP:142073417
482 482 c, t dbSNP:777049577
483 483 a, g dbSNP:147841606
496 496 a, c dbSNP:755252656
497 497 a, c, g dbSNP:543399762
503 503 a, c dbSNP:770299353
504 504 -, g dbSNP:34622961
513 513 c, t dbSNP:773617215
514 514 a, g, t dbSNP:749858367
516 516 a, g dbSNP:774069829
528 528 a, c dbSNP:759246142
550 550 a, g dbSNP:771810191
554 554 c, t dbSNP:775303808
564 564 g, t dbSNP:760423119
572 572 a, g dbSNP:763763889
573 573 c, t dbSNP:771252412
581 581 c, t dbSNP:541167867
588 588 a, g dbSNP:765164378
590 590 a, g dbSNP:751600633
592 592 c, t dbSNP:559481815
593 593 a, g dbSNP:369882771
601 601 a, t dbSNP:752834753
609 609 a, g dbSNP:146395267
620 620 a, g dbSNP:756422562
625 625 a, g dbSNP:141421714
629 629 c, t dbSNP:754241461
636 636 g, t dbSNP:757768757
637 637 c, t dbSNP:779593424
642 642 a, g dbSNP:745321921
646 646 a, g dbSNP:771685804
646 646 -, g dbSNP:755973091
664 664 a, g dbSNP:200549732
669 669 c, t dbSNP:548387738
683 683 a, g dbSNP:768368801
685 685 g, t dbSNP:746532969
690 690 c, t dbSNP:768156505
709 709 c, t dbSNP:780921835
722 722 c, t dbSNP:747849307
723 723 a, g dbSNP:370307569
733 733 a, g dbSNP:773061827
735 735 c, t dbSNP:147017172
736 736 a, g dbSNP:751022116
746 746 c, t dbSNP:536511561
748 748 a, g dbSNP:201294938
757 757 a, g dbSNP:760837124
758 758 c, t dbSNP:764171622
759 759 a, g dbSNP:372549728
771 771 a, g dbSNP:200392534
783 783 c, t dbSNP:765692468
785 785 c, t dbSNP:750832640
792 792 a, g dbSNP:778069510
794 794 c, g dbSNP:749516210
798 798 a, g dbSNP:370194438
803 803 a, g dbSNP:774715234
814 814 a, c dbSNP:759845948
825 825 a, g dbSNP:772516874
840 840 a, g dbSNP:375612298
848 848 a, g dbSNP:761209524
850 850 a, c dbSNP:763588874
859 859 g, t dbSNP:531508748
865 865 a, g dbSNP:761471633
866 866 -, g dbSNP:762204431
866 866 g, t dbSNP:77074957
868 868 a, g dbSNP:186875564
881 881 g, t dbSNP:750185209
895 895 a, g dbSNP:28362775
900 900 c, t dbSNP:368609319
917 917 c, t dbSNP:751540613
932 932 a, g dbSNP:755026676
946 946 a, g dbSNP:550268175
950 950 c, t dbSNP:777855403
952 952 g, t dbSNP:749336302
953 953 a, c dbSNP:757456469
954 954 c, t dbSNP:779021334
963 963 a, c, g dbSNP:150207791
965 965 c, t dbSNP:775897050
966 966 c, t dbSNP:558120815
972 972 g, t dbSNP:138805494
981 981 a, g dbSNP:149342451
991 991 c, t dbSNP:761251617
1005 1005 c, t dbSNP:764852134
1011 1011 c, t dbSNP:772709865
1014 1014 a, g dbSNP:762762265
1023 1023 c, t dbSNP:769102282
1024 1024 c, t dbSNP:776982710
1025 1025 a, g dbSNP:748731866
1035 1035 c, t dbSNP:769184887
1038 1038 c, t dbSNP:772839507
1041 1041 c, t dbSNP:762639586
1043 1043 c, t dbSNP:766138580
1046 1046 a, t dbSNP:774252448
1047 1047 a, c dbSNP:759421873
1049 1049 a, g dbSNP:376820760
1052 1052 a, g, t dbSNP:752542741
1058 1058 a, g dbSNP:765216094
1063 1063 c, t dbSNP:370525728
1065 1065 c, t dbSNP:758549167
1066 1066 c, g dbSNP:780286623
1079 1079 a, g dbSNP:751829593
1080 1080 c, t dbSNP:28362777
1081 1081 a, g dbSNP:544358450
1096 1096 c, g dbSNP:748591988
1100 1100 -, agct dbSNP:751552396
1101 1101 g, t dbSNP:770350333
1104 1104 a, g dbSNP:138248389
1109 1109 c, g dbSNP:1045644
1137 1137 c, g, t dbSNP:371011287
1142 1142 a, g dbSNP:774121028
1145 1145 a, c dbSNP:759299023
1146 1146 -, c dbSNP:36109457
1152 1152 a, g dbSNP:771756402
1157 1157 a, c, t dbSNP:375542487
1159 1159 c, g dbSNP:764096356
1169 1169 -, t dbSNP:755106768
1176 1176 a, c dbSNP:755142161
1182 1182 c, t dbSNP:146166304
1186 1186 a, g dbSNP:762998217
1187 1187 c, t dbSNP:370382942
1189 1189 a, g dbSNP:267603975
1190 1190 -, agat dbSNP:777627665
1194 1194 a, c, t dbSNP:577912568
1197 1197 a, c dbSNP:781355952
1200 1200 c, t dbSNP:753113360
1204 1204 c, t dbSNP:756541797
1205 1205 a, g dbSNP:545418366
1206 1206 c, t dbSNP:140108742
1210 1210 a, g dbSNP:770530713
1214 1214 a, g, t dbSNP:571941613
1221 1221 c, t dbSNP:77370101
1238 1238 a, g dbSNP:200904881
1242 1242 c, t dbSNP:771767136
1251 1251 a, c dbSNP:775079663
1253 1253 c, t dbSNP:760549722
1254 1254 a, g dbSNP:150529026
1255 1255 a, g dbSNP:776519637
1258 1258 a, g dbSNP:28362778
1259 1259 c, t dbSNP:766435124
1268 1268 a, c dbSNP:751654014
1271 1271 c, t dbSNP:748478920
1275 1275 c, t dbSNP:199608109
1293 1293 c, t dbSNP:767814589
1300 1300 c, t dbSNP:367884240
1301 1301 a, g dbSNP:772383772
1304 1304 c, t dbSNP:756473964
1305 1305 a, g dbSNP:549957619
1323 1323 c, g, t dbSNP:35353967
1324 1324 g, t dbSNP:61759484
1334 1334 a, g dbSNP:745390437
1340 1340 a, g dbSNP:757948966
1364 1364 g, t dbSNP:779778621
1367 1367 a, g dbSNP:746680829
1369 1369 a, t dbSNP:768435123
1370 1370 a, c dbSNP:776467069
1372 1372 a, g dbSNP:748019236
1373 1373 c, t dbSNP:769725783
1378 1378 c, g dbSNP:774252846
1381 1381 a, g dbSNP:759679894
1384 1384 a, g dbSNP:371248806
1402 1402 a, g dbSNP:139503327
1411 1411 a, g dbSNP:760876932
1412 1412 a, c dbSNP:764544231
1414 1414 g, t dbSNP:754271185
1418 1418 c, g dbSNP:757774488
1425 1425 a, g dbSNP:765878055
1430 1430 a, g dbSNP:371099832
1432 1432 c, t dbSNP:749854026
1490 1490 a, t dbSNP:757904271
1502 1502 a, g dbSNP:779496405
1505 1505 a, g dbSNP:746659336
1514 1514 c, g dbSNP:754636399
1537 1537 a, g dbSNP:770789480
1538 1538 c, g dbSNP:780116790
1539 1539 -, a dbSNP:779782495
1562 1562 g, t dbSNP:200252533
1565 1565 c, g dbSNP:768952355
1567 1567 a, c dbSNP:201578645
1569 1569 c, t dbSNP:762167606
1575 1575 a, t dbSNP:564563172
1589 1589 c, t dbSNP:121908934
1595 1595 c, t dbSNP:763449449
1597 1597 a, t dbSNP:766825368
1601 1601 a, c dbSNP:751018920
1602 1602 a, g dbSNP:747088615
1603 1603 a, g dbSNP:759022387
1607 1607 a, g dbSNP:17097468
1624 1624 a, c dbSNP:752337401
1625 1625 c, t dbSNP:755826383
1627 1627 -, agag dbSNP:746449866
1628 1628 g, t dbSNP:147643069
1636 1636 a, c dbSNP:753583403
1645 1645 c, g dbSNP:149072811
1648 1648 a, c, t dbSNP:1801963
1662 1662 c, t dbSNP:745850559
1669 1669 a, g dbSNP:768825582
1672 1672 c, g dbSNP:781479122
1673 1673 c, g dbSNP:748322615
1677 1677 c, t dbSNP:769911394
1679 1679 a, g, t dbSNP:121908932
1682 1682 a, g dbSNP:143142304
1688 1688 a, t dbSNP:763246563
1690 1690 c, t dbSNP:771372583
1701 1701 c, g dbSNP:774804924
1702 1702 a, c dbSNP:747736722
1703 1703 -, aat dbSNP:768219316
1740 1740 c, t dbSNP:747450536
1746 1746 a, c dbSNP:528157875
1747 1747 a, g dbSNP:539865179
1754 1754 -, aga dbSNP:758229492
1763 1763 c, t dbSNP:58432000
1848 1848 -, c dbSNP:750489098
1851 1851 -, aaaaaaaaaaaaaaa dbSNP:781011932
1851 1851 a, c dbSNP:766407450
1875 1875 a, g dbSNP:71415969
1876 1876 -, a dbSNP:35858438
1876 1876 -, a dbSNP:58905560

Target ORF information:

RefSeq Version XM_011536539
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cochlin (COCH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536539

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.