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PTCRA pre T-cell antigen receptor alpha [Homo sapiens (human)]

Gene Symbol PTCRA
Entrez Gene ID 171558
Full Name pre T-cell antigen receptor alpha
Synonyms PT-ALPHA, PTA
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. lac of sum
Disorder MIM:

606817

Disorder Html:





The following PTCRA gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCRA gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu55835 NM_001243170 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu77450 XM_011514316 PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu77451 XM_011514317 PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu18718 NM_001243168 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu55895 NM_001243169 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu29044 NM_138296 Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu55835
Accession Version NM_001243170.1
Sequence Information ORF Nucleotide Sequence (Length: 525bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2014
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, AF101436.1, BC153829.1 and AW014894.1. Summary: The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) lacks two internal segments compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF101436.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGA 
GAGGCTTCTA CAGCCAGGAC CTGCCCCCAG GAGCCTCTCA GGGGGACACC GGGTGGGGCG
CTGTGGCTGG GGGTCCTGCG GCTGCTGCTC TTCAAGCTGC TGCTGTTTGA CCTGCTCCTG
ACCTGCAGCT GCCTGTGCGA CCCCGCGGGC CCGCTGCCTT CCCCCGCAAC CACCACCCGC
CTGCGAGCCC TCGGCTCCCA TCGACTGCAC CCGGCCACGG AGACTGGGGG ACGAGAGGCC
ACCAGCTCAC CCAGACCCCA GCCTCGGGAC CGCCGCTGGG GTGACACCCC TCCGGGTCGG
AAGCCCGGGA GCCCAGTATG GGGGGAAGGG TCTTACCTCA GCAGTTACCC CACTTGCCCA
GCACAGGCCT GGTGCTCAAG ATCTGCCCTC AGGGCTCCTT CCTCCAGTCT TGGAGCATTT
TTTGCAGGTG ACCTGCCTCC TCCTCTGCAG GCTGGAGCTG CCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001230099.1
CDS82..606
Misc Feature(1)1..3(+)
Misc Feature(2)124..279(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..184
Gene:PTCRA
Gene Synonym:
Exon (3)185..762
Gene:PTCRA
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:552097755
13 13 c, t dbSNP:559446349
25 25 c, t dbSNP:374451302
27 27 c, t dbSNP:200306211
32 32 c, g, t dbSNP:772667489
33 33 a, c, g dbSNP:761067985
34 34 c, g dbSNP:754225849
36 36 c, t dbSNP:548177244
37 37 a, c, g, t dbSNP:779867569
39 39 a, c dbSNP:777637876
40 40 a, g dbSNP:568097162
56 56 a, g dbSNP:756935540
57 57 a, g dbSNP:781036234
68 68 c, g dbSNP:561719796
79 79 a, g, t dbSNP:745700204
81 81 c, t dbSNP:779886058
87 87 c, t dbSNP:774492937
88 88 a, g dbSNP:376557082
93 93 -, a dbSNP:749643841
93 93 a, c, g dbSNP:138459529
96 96 g, t dbSNP:188435679
114 114 a, c dbSNP:747517503
129 129 c, t dbSNP:771396393
137 137 c, t dbSNP:777028518
139 139 c, g dbSNP:768315052
154 154 c, g dbSNP:768503752
156 156 c, t dbSNP:548435231
160 160 a, g dbSNP:761444307
161 161 c, t dbSNP:767210145
181 181 a, g dbSNP:772682493
189 189 -, a dbSNP:763910876
191 191 c, t dbSNP:564467179
192 192 a, g dbSNP:753917737
193 193 a, c, g dbSNP:755076613
200 200 c, t dbSNP:752631531
201 201 a, g dbSNP:758393976
204 204 a, g dbSNP:533321958
206 206 a, g dbSNP:746826227
215 215 g, t dbSNP:756901784
220 220 c, t dbSNP:780737707
221 221 a, g, t dbSNP:745333497
241 241 c, t dbSNP:775207181
242 242 a, t dbSNP:547016440
252 252 c, t dbSNP:772757131
253 253 c, t dbSNP:773597378
266 266 a, g dbSNP:774026929
276 276 a, g dbSNP:760979735
279 279 c, g dbSNP:766577832
286 286 a, g dbSNP:776736708
287 287 a, c dbSNP:376897338
288 288 a, g dbSNP:765293082
293 293 c, t dbSNP:367893841
294 294 a, g dbSNP:184782115
306 306 c, t dbSNP:759156804
307 307 a, g dbSNP:36111725
313 313 a, c dbSNP:751330894
316 316 a, c dbSNP:757025642
319 319 c, t dbSNP:187988655
326 326 a, g dbSNP:111782749
334 334 a, g dbSNP:373866603
339 339 c, t dbSNP:779687830
340 340 c, t dbSNP:748845914
343 343 c, t dbSNP:542853935
344 344 c, g dbSNP:557469946
346 346 c, t dbSNP:747336644
347 347 c, t dbSNP:771200206
353 353 c, t dbSNP:571012981
354 354 a, g, t dbSNP:533516824
360 360 a, g dbSNP:775271650
373 373 c, t dbSNP:553747473
374 374 c, g dbSNP:775518759
381 381 c, t dbSNP:763053393
397 397 a, c dbSNP:764045039
406 406 c, t dbSNP:751466261
412 412 c, t dbSNP:200928800
413 413 a, g dbSNP:369843415
415 415 a, c, t dbSNP:201876282
426 426 c, t dbSNP:763955290
434 434 c, t dbSNP:373020262
435 435 a, g dbSNP:779882018
439 439 c, t dbSNP:375988717
440 440 a, g dbSNP:146531157
444 444 a, g dbSNP:544832101
445 445 c, g dbSNP:184524047
446 446 a, c, t dbSNP:578157654
447 447 c, t dbSNP:746273794
448 448 a, g dbSNP:769966432
449 449 a, g dbSNP:200924110
459 459 -, t dbSNP:751409607
459 459 a, g dbSNP:369095740
461 461 g, t dbSNP:550965943
462 462 c, g dbSNP:112142292
464 464 g, t dbSNP:774547744
469 469 a, g dbSNP:761633631
473 473 c, t dbSNP:61745373
478 478 c, t dbSNP:767528406
479 479 c, t dbSNP:750201200
480 480 -, a dbSNP:757539569
480 480 c, t dbSNP:760566709
485 485 c, g dbSNP:766069044
492 492 a, c dbSNP:753541240
505 505 a, c dbSNP:371204759
506 506 a, g dbSNP:778277201
510 510 c, t dbSNP:375457877
514 514 -, tgc dbSNP:781628496
514 514 c, t dbSNP:115331994
523 523 a, t dbSNP:368573599
526 526 c, g dbSNP:1997554
529 529 c, t dbSNP:267601038
532 532 a, g dbSNP:746183695
533 533 a, g dbSNP:370217795
535 535 a, g dbSNP:780183620
536 536 c, t dbSNP:768540118
552 552 c, t dbSNP:768971560
573 573 c, g dbSNP:774263017
588 588 a, g dbSNP:748288550
595 595 a, g dbSNP:372426467
599 599 c, t dbSNP:773293542
601 601 a, g dbSNP:61743125
608 608 g, t dbSNP:368137542
629 629 c, t dbSNP:776629714
630 630 a, g dbSNP:759092850
635 635 a, t dbSNP:764983525
637 637 -, tg dbSNP:750482976
672 672 -, c dbSNP:34259318
677 677 c, t dbSNP:562534073
705 705 a, g dbSNP:531362459
745 745 c, t dbSNP:528974068
746 746 g, t dbSNP:41274882

Target ORF information:

RefSeq Version NM_001243170
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243170

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGA 
GAGGCTTCTA CAGCCAGGAC CTGCCCCCAG GAGCCTCTCA GGGGGACACC GGGTGGGGCG
CTGTGGCTGG GGGTCCTGCG GCTGCTGCTC TTCAAGCTGC TGCTGTTTGA CCTGCTCCTG
ACCTGCAGCT GCCTGTGCGA CCCCGCGGGC CCGCTGCCTT CCCCCGCAAC CACCACCCGC
CTGCGAGCCC TCGGCTCCCA TCGACTGCAC CCGGCCACGG AGACTGGGGG ACGAGAGGCC
ACCAGCTCAC CCAGACCCCA GCCTCGGGAC CGCCGCTGGG GTGACACCCC TCCGGGTCGG
AAGCCCGGGA GCCCAGTATG GGGGGAAGGG TCTTACCTCA GCAGTTACCC CACTTGCCCA
GCACAGGCCT GGTGCTCAAG ATCTGCCCTC AGGGCTCCTT CCTCCAGTCT TGGAGCATTT
TTTGCAGGTG ACCTGCCTCC TCCTCTGCAG GCTGGAGCTG CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77450
Accession Version XM_011514316.1
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGCTGG TGGATGGAAA GCAGCAGATG GTGGTGGTCT GCCTGGTCCT TGATGTTGCA 
CCCCCTGGCC TTGACAGCCC CATCTGGTTC TCAGCCGGCA ATGGCAGTGC ACTGGATGCC
TTCACCTATG GCCCTTCCCC AGCAACGGAT GGCACCTGGA CCAACTTGGC CCATCTCTCC
CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG CCTTTGGTCT GCCACACTGG GCCTGGGGCT
GAGGGTCACA GCAGGAGTAC ACAGCCCATG CATCTGTCAG GAGAGGCTTC TACAGCCAGG
ACCTGCCCCC AGGAGCCTCT CAGGGGGACA CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG
CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT GACCTGCTCC TGACCTGCAG CTGCCTGTGC
GACCCCGCGG GCCCGCTGCC TTCCCCCGCA ACCACCACCC GCCTGCGAGC CCTCGGCTCC
CATCGACTGC ACCCGGCCAC GGAGACTGGG GGACGAGAGG CCACCAGCTC ACCCAGACCC
CAGCCTCGGG ACCGCCGCTG GGGTGACACC CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA
TGGGGGGAAG GGTCTTACCT CAGCAGTTAC CCCACTTGCC CAGCACAGGC CTGGTGCTCA
AGATCTGCCC TCAGGGCTCC TTCCTCCAGT CTTGGAGCAT TTTTTGCAGG TGACCTGCCT
CCTCCTCTGC AGGCTGGAGC TGCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512618.1
CDS132..878
Misc Feature(1)132..551(+)
Translation
Position Chain Variation Link
20 20 -, a dbSNP:575063807
20 20 c, g dbSNP:554805876
33 33 -, aag dbSNP:200785267
35 35 a, g dbSNP:112577452
98 98 c, t dbSNP:530346250
99 99 a, g dbSNP:768821077
105 105 c, t dbSNP:750343059
107 107 c, g dbSNP:143960660
111 111 -, c dbSNP:760254872
111 111 c, t dbSNP:766274816
117 117 c, g dbSNP:753748329
120 120 g, t dbSNP:371971484
121 121 c, t dbSNP:778744161
127 127 c, g dbSNP:747651022
129 129 a, g dbSNP:199894504
139 139 g, t dbSNP:779277349
147 147 a, g dbSNP:757865292
150 150 a, g dbSNP:781681295
154 154 a, t dbSNP:746364033
157 157 a, t dbSNP:770351672
167 167 c, g dbSNP:780382863
170 170 c, t dbSNP:373111406
177 177 a, g, t dbSNP:768826241
179 179 c, t dbSNP:761896723
183 183 a, g dbSNP:772052468
184 184 a, c dbSNP:200942121
186 186 a, g dbSNP:760520383
192 192 c, g, t dbSNP:146960695
209 209 a, c, g dbSNP:372102699
212 212 c, t dbSNP:752303292
213 213 a, t dbSNP:757857439
227 227 c, t dbSNP:137970521
239 239 c, t dbSNP:751138873
240 240 c, g dbSNP:756591065
244 244 c, t dbSNP:780570974
245 245 a, g dbSNP:749605990
246 246 a, g dbSNP:769024317
251 251 c, t dbSNP:779125923
254 254 c, t dbSNP:748318011
259 259 a, g dbSNP:141630791
277 277 c, t dbSNP:773148448
278 278 a, g dbSNP:760873532
290 290 c, g dbSNP:770947767
300 300 a, g dbSNP:201708224
305 305 a, t dbSNP:759343944
315 315 c, t dbSNP:765126989
317 317 g, t dbSNP:752602270
327 327 c, g, t dbSNP:147986652
335 335 c, t dbSNP:750993939
342 342 c, g dbSNP:150508492
348 348 a, g dbSNP:9471966
356 356 c, t dbSNP:201816889
375 375 g, t dbSNP:754349321
376 376 g, t dbSNP:755548523
380 380 c, g dbSNP:200182239
382 382 a, g dbSNP:748599249
392 392 a, c dbSNP:758521719
396 396 c, t dbSNP:778083552
397 397 c, t dbSNP:747032918
402 402 c, t dbSNP:771146026
403 403 a, c, t dbSNP:141979329
426 426 c, g dbSNP:768503752
428 428 c, t dbSNP:548435231
432 432 a, g dbSNP:761444307
433 433 c, t dbSNP:767210145
453 453 a, g dbSNP:772682493
461 461 -, a dbSNP:763910876
463 463 c, t dbSNP:564467179
464 464 a, g dbSNP:753917737
465 465 a, c, g dbSNP:755076613
472 472 c, t dbSNP:752631531
473 473 a, g dbSNP:758393976
476 476 a, g dbSNP:533321958
478 478 a, g dbSNP:746826227
487 487 g, t dbSNP:756901784
492 492 c, t dbSNP:780737707
493 493 a, g, t dbSNP:745333497
513 513 c, t dbSNP:775207181
514 514 a, t dbSNP:547016440
524 524 c, t dbSNP:772757131
525 525 c, t dbSNP:773597378
538 538 a, g dbSNP:774026929
548 548 a, g dbSNP:760979735
551 551 c, g dbSNP:766577832
558 558 a, g dbSNP:776736708
559 559 a, c dbSNP:376897338
560 560 a, g dbSNP:765293082
565 565 c, t dbSNP:367893841
566 566 a, g dbSNP:184782115
578 578 c, t dbSNP:759156804
579 579 a, g dbSNP:36111725
585 585 a, c dbSNP:751330894
588 588 a, c dbSNP:757025642
591 591 c, t dbSNP:187988655
598 598 a, g dbSNP:111782749
606 606 a, g dbSNP:373866603
611 611 c, t dbSNP:779687830
612 612 c, t dbSNP:748845914
615 615 c, t dbSNP:542853935
616 616 c, g dbSNP:557469946
618 618 c, t dbSNP:747336644
619 619 c, t dbSNP:771200206
625 625 c, t dbSNP:571012981
626 626 a, g, t dbSNP:533516824
632 632 a, g dbSNP:775271650
645 645 c, t dbSNP:553747473
646 646 c, g dbSNP:775518759
653 653 c, t dbSNP:763053393
669 669 a, c dbSNP:764045039
678 678 c, t dbSNP:751466261
684 684 c, t dbSNP:200928800
685 685 a, g dbSNP:369843415
687 687 a, c, t dbSNP:201876282
698 698 c, t dbSNP:763955290
706 706 c, t dbSNP:373020262
707 707 a, g dbSNP:779882018
711 711 c, t dbSNP:375988717
712 712 a, g dbSNP:146531157
716 716 a, g dbSNP:544832101
717 717 c, g dbSNP:184524047
718 718 a, c, t dbSNP:578157654
719 719 c, t dbSNP:746273794
720 720 a, g dbSNP:769966432
721 721 a, g dbSNP:200924110
731 731 -, t dbSNP:751409607
731 731 a, g dbSNP:369095740
733 733 g, t dbSNP:550965943
734 734 c, g dbSNP:112142292
736 736 g, t dbSNP:774547744
741 741 a, g dbSNP:761633631
745 745 c, t dbSNP:61745373
750 750 c, t dbSNP:767528406
751 751 c, t dbSNP:750201200
752 752 -, a dbSNP:757539569
752 752 c, t dbSNP:760566709
757 757 c, g dbSNP:766069044
764 764 a, c dbSNP:753541240
777 777 a, c dbSNP:371204759
778 778 a, g dbSNP:778277201
782 782 c, t dbSNP:375457877
786 786 -, tgc dbSNP:781628496
786 786 c, t dbSNP:115331994
795 795 a, t dbSNP:368573599
798 798 c, g dbSNP:1997554
801 801 c, t dbSNP:267601038
804 804 a, g dbSNP:746183695
805 805 a, g dbSNP:370217795
807 807 a, g dbSNP:780183620
808 808 c, t dbSNP:768540118
824 824 c, t dbSNP:768971560
845 845 c, g dbSNP:774263017
860 860 a, g dbSNP:748288550
867 867 a, g dbSNP:372426467
871 871 c, t dbSNP:773293542
873 873 a, g dbSNP:61743125
880 880 g, t dbSNP:368137542
901 901 c, t dbSNP:776629714
902 902 a, g dbSNP:759092850
907 907 a, t dbSNP:764983525
909 909 -, tg dbSNP:750482976
944 944 -, c dbSNP:34259318
949 949 c, t dbSNP:562534073
977 977 a, g dbSNP:531362459
1017 1017 c, t dbSNP:528974068
1018 1018 g, t dbSNP:41274882

Target ORF information:

RefSeq Version XM_011514316
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514316

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGCTGG TGGATGGAAA GCAGCAGATG GTGGTGGTCT GCCTGGTCCT TGATGTTGCA 
CCCCCTGGCC TTGACAGCCC CATCTGGTTC TCAGCCGGCA ATGGCAGTGC ACTGGATGCC
TTCACCTATG GCCCTTCCCC AGCAACGGAT GGCACCTGGA CCAACTTGGC CCATCTCTCC
CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG CCTTTGGTCT GCCACACTGG GCCTGGGGCT
GAGGGTCACA GCAGGAGTAC ACAGCCCATG CATCTGTCAG GAGAGGCTTC TACAGCCAGG
ACCTGCCCCC AGGAGCCTCT CAGGGGGACA CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG
CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT GACCTGCTCC TGACCTGCAG CTGCCTGTGC
GACCCCGCGG GCCCGCTGCC TTCCCCCGCA ACCACCACCC GCCTGCGAGC CCTCGGCTCC
CATCGACTGC ACCCGGCCAC GGAGACTGGG GGACGAGAGG CCACCAGCTC ACCCAGACCC
CAGCCTCGGG ACCGCCGCTG GGGTGACACC CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA
TGGGGGGAAG GGTCTTACCT CAGCAGTTAC CCCACTTGCC CAGCACAGGC CTGGTGCTCA
AGATCTGCCC TCAGGGCTCC TTCCTCCAGT CTTGGAGCAT TTTTTGCAGG TGACCTGCCT
CCTCCTCTGC AGGCTGGAGC TGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77451
Accession Version XM_011514317.1
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007592.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGCTA CTTCTCCTGG CCCTTGGGTG TCCAGCCCTA CCCACAGCCC CATCTGGTTC 
TCAGCCGGCA ATGGCAGTGC ACTGGATGCC TTCACCTATG GCCCTTCCCC AGCAACGGAT
GGCACCTGGA CCAACTTGGC CCATCTCTCC CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG
CCTTTGGTCT GCCACACTGG GCCTGGGGCT GAGGGTCACA GCAGGAGTAC ACAGCCCATG
CATCTGTCAG GAGAGGCTTC TACAGCCAGG ACCTGCCCCC AGGAGCCTCT CAGGGGGACA
CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT
GACCTGCTCC TGACCTGCAG CTGCCTGTGC GACCCCGCGG GCCCGCTGCC TTCCCCCGCA
ACCACCACCC GCCTGCGAGC CCTCGGCTCC CATCGACTGC ACCCGGCCAC GGAGACTGGG
GGACGAGAGG CCACCAGCTC ACCCAGACCC CAGCCTCGGG ACCGCCGCTG GGGTGACACC
CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA TGGGGGGAAG GGTCTTACCT CAGCAGTTAC
CCCACTTGCC CAGCACAGGC CTGGTGCTCA AGATCTGCCC TCAGGGCTCC TTCCTCCAGT
CTTGGAGCAT TTTTTGCAGG TGACCTGCCT CCTCCTCTGC AGGCTGGAGC TGCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011512619.1
CDS275..991
Misc Feature(1)320..664(+)
Translation
Position Chain Variation Link
31 31 g, t dbSNP:537132466
55 55 a, g dbSNP:560968210
81 81 a, g dbSNP:368662481
125 125 c, t dbSNP:9471958
134 134 g, t dbSNP:563314273
145 145 c, t dbSNP:532381683
189 189 a, g dbSNP:552097755
195 195 c, t dbSNP:559446349
207 207 c, t dbSNP:374451302
209 209 c, t dbSNP:200306211
214 214 c, g, t dbSNP:772667489
215 215 a, c, g dbSNP:761067985
216 216 c, g dbSNP:754225849
218 218 c, t dbSNP:548177244
219 219 a, c, g, t dbSNP:779867569
221 221 a, c dbSNP:777637876
222 222 a, g dbSNP:568097162
238 238 a, g dbSNP:756935540
239 239 a, g dbSNP:781036234
250 250 c, g dbSNP:561719796
261 261 a, g, t dbSNP:745700204
263 263 c, t dbSNP:779886058
269 269 c, t dbSNP:774492937
270 270 a, g dbSNP:376557082
275 275 -, a dbSNP:749643841
275 275 a, c, g dbSNP:138459529
278 278 g, t dbSNP:188435679
296 296 a, c dbSNP:747517503
311 311 c, t dbSNP:771396393
319 319 c, t dbSNP:777028518
321 321 c, g dbSNP:768315052
322 322 a, c, g dbSNP:372102699
325 325 c, t dbSNP:752303292
326 326 a, t dbSNP:757857439
340 340 c, t dbSNP:137970521
352 352 c, t dbSNP:751138873
353 353 c, g dbSNP:756591065
357 357 c, t dbSNP:780570974
358 358 a, g dbSNP:749605990
359 359 a, g dbSNP:769024317
364 364 c, t dbSNP:779125923
367 367 c, t dbSNP:748318011
372 372 a, g dbSNP:141630791
390 390 c, t dbSNP:773148448
391 391 a, g dbSNP:760873532
403 403 c, g dbSNP:770947767
413 413 a, g dbSNP:201708224
418 418 a, t dbSNP:759343944
428 428 c, t dbSNP:765126989
430 430 g, t dbSNP:752602270
440 440 c, g, t dbSNP:147986652
448 448 c, t dbSNP:750993939
455 455 c, g dbSNP:150508492
461 461 a, g dbSNP:9471966
469 469 c, t dbSNP:201816889
488 488 g, t dbSNP:754349321
489 489 g, t dbSNP:755548523
493 493 c, g dbSNP:200182239
495 495 a, g dbSNP:748599249
505 505 a, c dbSNP:758521719
509 509 c, t dbSNP:778083552
510 510 c, t dbSNP:747032918
515 515 c, t dbSNP:771146026
516 516 a, c, t dbSNP:141979329
539 539 c, g dbSNP:768503752
541 541 c, t dbSNP:548435231
545 545 a, g dbSNP:761444307
546 546 c, t dbSNP:767210145
566 566 a, g dbSNP:772682493
574 574 -, a dbSNP:763910876
576 576 c, t dbSNP:564467179
577 577 a, g dbSNP:753917737
578 578 a, c, g dbSNP:755076613
585 585 c, t dbSNP:752631531
586 586 a, g dbSNP:758393976
589 589 a, g dbSNP:533321958
591 591 a, g dbSNP:746826227
600 600 g, t dbSNP:756901784
605 605 c, t dbSNP:780737707
606 606 a, g, t dbSNP:745333497
626 626 c, t dbSNP:775207181
627 627 a, t dbSNP:547016440
637 637 c, t dbSNP:772757131
638 638 c, t dbSNP:773597378
651 651 a, g dbSNP:774026929
661 661 a, g dbSNP:760979735
664 664 c, g dbSNP:766577832
671 671 a, g dbSNP:776736708
672 672 a, c dbSNP:376897338
673 673 a, g dbSNP:765293082
678 678 c, t dbSNP:367893841
679 679 a, g dbSNP:184782115
691 691 c, t dbSNP:759156804
692 692 a, g dbSNP:36111725
698 698 a, c dbSNP:751330894
701 701 a, c dbSNP:757025642
704 704 c, t dbSNP:187988655
711 711 a, g dbSNP:111782749
719 719 a, g dbSNP:373866603
724 724 c, t dbSNP:779687830
725 725 c, t dbSNP:748845914
728 728 c, t dbSNP:542853935
729 729 c, g dbSNP:557469946
731 731 c, t dbSNP:747336644
732 732 c, t dbSNP:771200206
738 738 c, t dbSNP:571012981
739 739 a, g, t dbSNP:533516824
745 745 a, g dbSNP:775271650
758 758 c, t dbSNP:553747473
759 759 c, g dbSNP:775518759
766 766 c, t dbSNP:763053393
782 782 a, c dbSNP:764045039
791 791 c, t dbSNP:751466261
797 797 c, t dbSNP:200928800
798 798 a, g dbSNP:369843415
800 800 a, c, t dbSNP:201876282
811 811 c, t dbSNP:763955290
819 819 c, t dbSNP:373020262
820 820 a, g dbSNP:779882018
824 824 c, t dbSNP:375988717
825 825 a, g dbSNP:146531157
829 829 a, g dbSNP:544832101
830 830 c, g dbSNP:184524047
831 831 a, c, t dbSNP:578157654
832 832 c, t dbSNP:746273794
833 833 a, g dbSNP:769966432
834 834 a, g dbSNP:200924110
844 844 -, t dbSNP:751409607
844 844 a, g dbSNP:369095740
846 846 g, t dbSNP:550965943
847 847 c, g dbSNP:112142292
849 849 g, t dbSNP:774547744
854 854 a, g dbSNP:761633631
858 858 c, t dbSNP:61745373
863 863 c, t dbSNP:767528406
864 864 c, t dbSNP:750201200
865 865 -, a dbSNP:757539569
865 865 c, t dbSNP:760566709
870 870 c, g dbSNP:766069044
877 877 a, c dbSNP:753541240
890 890 a, c dbSNP:371204759
891 891 a, g dbSNP:778277201
895 895 c, t dbSNP:375457877
899 899 -, tgc dbSNP:781628496
899 899 c, t dbSNP:115331994
908 908 a, t dbSNP:368573599
911 911 c, g dbSNP:1997554
914 914 c, t dbSNP:267601038
917 917 a, g dbSNP:746183695
918 918 a, g dbSNP:370217795
920 920 a, g dbSNP:780183620
921 921 c, t dbSNP:768540118
937 937 c, t dbSNP:768971560
958 958 c, g dbSNP:774263017
973 973 a, g dbSNP:748288550
980 980 a, g dbSNP:372426467
984 984 c, t dbSNP:773293542
986 986 a, g dbSNP:61743125
993 993 g, t dbSNP:368137542
1014 1014 c, t dbSNP:776629714
1015 1015 a, g dbSNP:759092850
1020 1020 a, t dbSNP:764983525
1022 1022 -, tg dbSNP:750482976
1057 1057 -, c dbSNP:34259318
1062 1062 c, t dbSNP:562534073
1090 1090 a, g dbSNP:531362459
1130 1130 c, t dbSNP:528974068
1131 1131 g, t dbSNP:41274882

Target ORF information:

RefSeq Version XM_011514317
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514317

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGCTA CTTCTCCTGG CCCTTGGGTG TCCAGCCCTA CCCACAGCCC CATCTGGTTC 
TCAGCCGGCA ATGGCAGTGC ACTGGATGCC TTCACCTATG GCCCTTCCCC AGCAACGGAT
GGCACCTGGA CCAACTTGGC CCATCTCTCC CTGCCTTCTG AGGAGCTGGC ATCCTGGGAG
CCTTTGGTCT GCCACACTGG GCCTGGGGCT GAGGGTCACA GCAGGAGTAC ACAGCCCATG
CATCTGTCAG GAGAGGCTTC TACAGCCAGG ACCTGCCCCC AGGAGCCTCT CAGGGGGACA
CCGGGTGGGG CGCTGTGGCT GGGGGTCCTG CGGCTGCTGC TCTTCAAGCT GCTGCTGTTT
GACCTGCTCC TGACCTGCAG CTGCCTGTGC GACCCCGCGG GCCCGCTGCC TTCCCCCGCA
ACCACCACCC GCCTGCGAGC CCTCGGCTCC CATCGACTGC ACCCGGCCAC GGAGACTGGG
GGACGAGAGG CCACCAGCTC ACCCAGACCC CAGCCTCGGG ACCGCCGCTG GGGTGACACC
CCTCCGGGTC GGAAGCCCGG GAGCCCAGTA TGGGGGGAAG GGTCTTACCT CAGCAGTTAC
CCCACTTGCC CAGCACAGGC CTGGTGCTCA AGATCTGCCC TCAGGGCTCC TTCCTCCAGT
CTTGGAGCAT TTTTTGCAGG TGACCTGCCT CCTCCTCTGC AGGCTGGAGC TGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18718
Accession Version NM_001243168.1
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, AK130819.1, BC153829.1 and AW014894.1. This sequence is a reference standard in the RefSeqGene project. Summary: The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BP367764.1, BP367764.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGGGCT GCGGGCTCCT GCGGGCTCCT GAGCGGTTCC TCCTCGCAGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001230097.1
CDS82..972
Misc Feature(1)1..3(+)
Misc Feature(2)139..645(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..460
Gene:PTCRA
Gene Synonym:
Exon (3)461..505
Gene:PTCRA
Gene Synonym:
Exon (4)506..1128
Gene:PTCRA
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:552097755
13 13 c, t dbSNP:559446349
25 25 c, t dbSNP:374451302
27 27 c, t dbSNP:200306211
32 32 c, g, t dbSNP:772667489
33 33 a, c, g dbSNP:761067985
34 34 c, g dbSNP:754225849
36 36 c, t dbSNP:548177244
37 37 a, c, g, t dbSNP:779867569
39 39 a, c dbSNP:777637876
40 40 a, g dbSNP:568097162
56 56 a, g dbSNP:756935540
57 57 a, g dbSNP:781036234
68 68 c, g dbSNP:561719796
79 79 a, g, t dbSNP:745700204
81 81 c, t dbSNP:779886058
87 87 c, t dbSNP:774492937
88 88 a, g dbSNP:376557082
93 93 -, a dbSNP:749643841
93 93 a, c, g dbSNP:138459529
96 96 g, t dbSNP:188435679
114 114 a, c dbSNP:747517503
129 129 c, t dbSNP:771396393
137 137 c, t dbSNP:777028518
139 139 c, g dbSNP:768315052
147 147 c, t dbSNP:530346250
148 148 a, g dbSNP:768821077
154 154 c, t dbSNP:750343059
156 156 c, g dbSNP:143960660
160 160 -, c dbSNP:760254872
160 160 c, t dbSNP:766274816
166 166 c, g dbSNP:753748329
169 169 g, t dbSNP:371971484
170 170 c, t dbSNP:778744161
176 176 c, g dbSNP:747651022
178 178 a, g dbSNP:199894504
188 188 g, t dbSNP:779277349
196 196 a, g dbSNP:757865292
199 199 a, g dbSNP:781681295
203 203 a, t dbSNP:746364033
206 206 a, t dbSNP:770351672
216 216 c, g dbSNP:780382863
219 219 c, t dbSNP:373111406
226 226 a, g, t dbSNP:768826241
228 228 c, t dbSNP:761896723
232 232 a, g dbSNP:772052468
233 233 a, c dbSNP:200942121
235 235 a, g dbSNP:760520383
241 241 c, g, t dbSNP:146960695
258 258 a, c, g dbSNP:372102699
261 261 c, t dbSNP:752303292
262 262 a, t dbSNP:757857439
276 276 c, t dbSNP:137970521
288 288 c, t dbSNP:751138873
289 289 c, g dbSNP:756591065
293 293 c, t dbSNP:780570974
294 294 a, g dbSNP:749605990
295 295 a, g dbSNP:769024317
300 300 c, t dbSNP:779125923
303 303 c, t dbSNP:748318011
308 308 a, g dbSNP:141630791
326 326 c, t dbSNP:773148448
327 327 a, g dbSNP:760873532
339 339 c, g dbSNP:770947767
349 349 a, g dbSNP:201708224
354 354 a, t dbSNP:759343944
364 364 c, t dbSNP:765126989
366 366 g, t dbSNP:752602270
376 376 c, g, t dbSNP:147986652
384 384 c, t dbSNP:750993939
391 391 c, g dbSNP:150508492
397 397 a, g dbSNP:9471966
405 405 c, t dbSNP:201816889
424 424 g, t dbSNP:754349321
425 425 g, t dbSNP:755548523
429 429 c, g dbSNP:200182239
431 431 a, g dbSNP:748599249
441 441 a, c dbSNP:758521719
445 445 c, t dbSNP:778083552
446 446 c, t dbSNP:747032918
451 451 c, t dbSNP:771146026
452 452 a, c, t dbSNP:141979329
475 475 c, g dbSNP:768503752
477 477 c, t dbSNP:548435231
481 481 a, g dbSNP:761444307
482 482 c, t dbSNP:767210145
502 502 a, g dbSNP:772682493
506 506 g, t dbSNP:573503571
509 509 a, c, g dbSNP:542024351
510 510 c, t dbSNP:749822925
511 511 c, t dbSNP:754915777
512 512 a, g dbSNP:778856943
513 513 c, t dbSNP:368405128
514 514 a, g dbSNP:199980862
518 518 c, t dbSNP:777336012
523 523 c, t dbSNP:746665812
527 527 c, t dbSNP:770545961
534 534 a, g dbSNP:776182353
536 536 a, g dbSNP:759007225
537 537 g, t dbSNP:769244842
538 538 c, t dbSNP:775020904
546 546 c, t dbSNP:544766356
547 547 a, g, t dbSNP:767990272
549 549 a, g dbSNP:760931226
555 555 -, a dbSNP:763910876
557 557 c, t dbSNP:564467179
558 558 a, g dbSNP:753917737
559 559 a, c, g dbSNP:755076613
566 566 c, t dbSNP:752631531
567 567 a, g dbSNP:758393976
570 570 a, g dbSNP:533321958
572 572 a, g dbSNP:746826227
581 581 g, t dbSNP:756901784
586 586 c, t dbSNP:780737707
587 587 a, g, t dbSNP:745333497
607 607 c, t dbSNP:775207181
608 608 a, t dbSNP:547016440
618 618 c, t dbSNP:772757131
619 619 c, t dbSNP:773597378
632 632 a, g dbSNP:774026929
642 642 a, g dbSNP:760979735
645 645 c, g dbSNP:766577832
652 652 a, g dbSNP:776736708
653 653 a, c dbSNP:376897338
654 654 a, g dbSNP:765293082
659 659 c, t dbSNP:367893841
660 660 a, g dbSNP:184782115
672 672 c, t dbSNP:759156804
673 673 a, g dbSNP:36111725
679 679 a, c dbSNP:751330894
682 682 a, c dbSNP:757025642
685 685 c, t dbSNP:187988655
692 692 a, g dbSNP:111782749
700 700 a, g dbSNP:373866603
705 705 c, t dbSNP:779687830
706 706 c, t dbSNP:748845914
709 709 c, t dbSNP:542853935
710 710 c, g dbSNP:557469946
712 712 c, t dbSNP:747336644
713 713 c, t dbSNP:771200206
719 719 c, t dbSNP:571012981
720 720 a, g, t dbSNP:533516824
726 726 a, g dbSNP:775271650
739 739 c, t dbSNP:553747473
740 740 c, g dbSNP:775518759
747 747 c, t dbSNP:763053393
763 763 a, c dbSNP:764045039
772 772 c, t dbSNP:751466261
778 778 c, t dbSNP:200928800
779 779 a, g dbSNP:369843415
781 781 a, c, t dbSNP:201876282
792 792 c, t dbSNP:763955290
800 800 c, t dbSNP:373020262
801 801 a, g dbSNP:779882018
805 805 c, t dbSNP:375988717
806 806 a, g dbSNP:146531157
810 810 a, g dbSNP:544832101
811 811 c, g dbSNP:184524047
812 812 a, c, t dbSNP:578157654
813 813 c, t dbSNP:746273794
814 814 a, g dbSNP:769966432
815 815 a, g dbSNP:200924110
825 825 -, t dbSNP:751409607
825 825 a, g dbSNP:369095740
827 827 g, t dbSNP:550965943
828 828 c, g dbSNP:112142292
830 830 g, t dbSNP:774547744
835 835 a, g dbSNP:761633631
839 839 c, t dbSNP:61745373
844 844 c, t dbSNP:767528406
845 845 c, t dbSNP:750201200
846 846 -, a dbSNP:757539569
846 846 c, t dbSNP:760566709
851 851 c, g dbSNP:766069044
858 858 a, c dbSNP:753541240
871 871 a, c dbSNP:371204759
872 872 a, g dbSNP:778277201
876 876 c, t dbSNP:375457877
880 880 -, tgc dbSNP:781628496
880 880 c, t dbSNP:115331994
889 889 a, t dbSNP:368573599
892 892 c, g dbSNP:1997554
895 895 c, t dbSNP:267601038
898 898 a, g dbSNP:746183695
899 899 a, g dbSNP:370217795
901 901 a, g dbSNP:780183620
902 902 c, t dbSNP:768540118
918 918 c, t dbSNP:768971560
939 939 c, g dbSNP:774263017
954 954 a, g dbSNP:748288550
961 961 a, g dbSNP:372426467
965 965 c, t dbSNP:773293542
967 967 a, g dbSNP:61743125
974 974 g, t dbSNP:368137542
995 995 c, t dbSNP:776629714
996 996 a, g dbSNP:759092850
1001 1001 a, t dbSNP:764983525
1003 1003 -, tg dbSNP:750482976
1038 1038 -, c dbSNP:34259318
1043 1043 c, t dbSNP:562534073
1071 1071 a, g dbSNP:531362459
1111 1111 c, t dbSNP:528974068
1112 1112 g, t dbSNP:41274882

Target ORF information:

RefSeq Version NM_001243168
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243168

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGGGCT GCGGGCTCCT GCGGGCTCCT GAGCGGTTCC TCCTCGCAGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu55895
Accession Version NM_001243169.1
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, AF165312.1, BC153829.1 and AW014894.1. Summary: The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. Transcript Variant: This variant (3) uses alternate in-frame splice junctions at the 5' ends of two exons and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) contains an alternate internal segment but lacks two others compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF165312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152719 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
CCTGTTTCCT TCCCTTCCTC ACCAGAGGCT GCCACAACAG GCCCCATCTG GTTCTCAGCC
GGCAATGGCA GTGCACTGGA TGCCTTCACC TATGGCCCTT CCCCAGCAAC GGATGGCACC
TGGACCAACT TGGCCCATCT CTCCCTGCCT TCTGAGGAGC TGGCATCCTG GGAGCCTTTG
GTCTGCCACA CTGGGCCTGG GGCTGAGGGT CACAGCAGGA GTACACAGCC CATGCATCTG
TCAGGAGAGG CTTCTACAGC CAGGACCTGC CCCCAGGAGC CTCTCAGGGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001230098.1
CDS82..852
Misc Feature(1)1..3(+)
Misc Feature(2)184..525(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..182
Gene:PTCRA
Gene Synonym:
Exon (3)183..385
Gene:PTCRA
Gene Synonym:
Exon (4)386..430
Gene:PTCRA
Gene Synonym:
Exon (5)431..1008
Gene:PTCRA
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:552097755
13 13 c, t dbSNP:559446349
25 25 c, t dbSNP:374451302
27 27 c, t dbSNP:200306211
32 32 c, g, t dbSNP:772667489
33 33 a, c, g dbSNP:761067985
34 34 c, g dbSNP:754225849
36 36 c, t dbSNP:548177244
37 37 a, c, g, t dbSNP:779867569
39 39 a, c dbSNP:777637876
40 40 a, g dbSNP:568097162
56 56 a, g dbSNP:756935540
57 57 a, g dbSNP:781036234
68 68 c, g dbSNP:561719796
79 79 a, g, t dbSNP:745700204
81 81 c, t dbSNP:779886058
87 87 c, t dbSNP:774492937
88 88 a, g dbSNP:376557082
93 93 -, a dbSNP:749643841
93 93 a, c, g dbSNP:138459529
96 96 g, t dbSNP:188435679
114 114 a, c dbSNP:747517503
129 129 c, t dbSNP:771396393
137 137 c, t dbSNP:777028518
139 139 c, g dbSNP:768315052
147 147 c, t dbSNP:748956235
183 183 a, c, g dbSNP:372102699
186 186 c, t dbSNP:752303292
187 187 a, t dbSNP:757857439
201 201 c, t dbSNP:137970521
213 213 c, t dbSNP:751138873
214 214 c, g dbSNP:756591065
218 218 c, t dbSNP:780570974
219 219 a, g dbSNP:749605990
220 220 a, g dbSNP:769024317
225 225 c, t dbSNP:779125923
228 228 c, t dbSNP:748318011
233 233 a, g dbSNP:141630791
251 251 c, t dbSNP:773148448
252 252 a, g dbSNP:760873532
264 264 c, g dbSNP:770947767
274 274 a, g dbSNP:201708224
279 279 a, t dbSNP:759343944
289 289 c, t dbSNP:765126989
291 291 g, t dbSNP:752602270
301 301 c, g, t dbSNP:147986652
309 309 c, t dbSNP:750993939
316 316 c, g dbSNP:150508492
322 322 a, g dbSNP:9471966
330 330 c, t dbSNP:201816889
349 349 g, t dbSNP:754349321
350 350 g, t dbSNP:755548523
354 354 c, g dbSNP:200182239
356 356 a, g dbSNP:748599249
366 366 a, c dbSNP:758521719
370 370 c, t dbSNP:778083552
371 371 c, t dbSNP:747032918
376 376 c, t dbSNP:771146026
377 377 a, c, t dbSNP:141979329
400 400 c, g dbSNP:768503752
402 402 c, t dbSNP:548435231
406 406 a, g dbSNP:761444307
407 407 c, t dbSNP:767210145
427 427 a, g dbSNP:772682493
435 435 -, a dbSNP:763910876
437 437 c, t dbSNP:564467179
438 438 a, g dbSNP:753917737
439 439 a, c, g dbSNP:755076613
446 446 c, t dbSNP:752631531
447 447 a, g dbSNP:758393976
450 450 a, g dbSNP:533321958
452 452 a, g dbSNP:746826227
461 461 g, t dbSNP:756901784
466 466 c, t dbSNP:780737707
467 467 a, g, t dbSNP:745333497
487 487 c, t dbSNP:775207181
488 488 a, t dbSNP:547016440
498 498 c, t dbSNP:772757131
499 499 c, t dbSNP:773597378
512 512 a, g dbSNP:774026929
522 522 a, g dbSNP:760979735
525 525 c, g dbSNP:766577832
532 532 a, g dbSNP:776736708
533 533 a, c dbSNP:376897338
534 534 a, g dbSNP:765293082
539 539 c, t dbSNP:367893841
540 540 a, g dbSNP:184782115
552 552 c, t dbSNP:759156804
553 553 a, g dbSNP:36111725
559 559 a, c dbSNP:751330894
562 562 a, c dbSNP:757025642
565 565 c, t dbSNP:187988655
572 572 a, g dbSNP:111782749
580 580 a, g dbSNP:373866603
585 585 c, t dbSNP:779687830
586 586 c, t dbSNP:748845914
589 589 c, t dbSNP:542853935
590 590 c, g dbSNP:557469946
592 592 c, t dbSNP:747336644
593 593 c, t dbSNP:771200206
599 599 c, t dbSNP:571012981
600 600 a, g, t dbSNP:533516824
606 606 a, g dbSNP:775271650
619 619 c, t dbSNP:553747473
620 620 c, g dbSNP:775518759
627 627 c, t dbSNP:763053393
643 643 a, c dbSNP:764045039
652 652 c, t dbSNP:751466261
658 658 c, t dbSNP:200928800
659 659 a, g dbSNP:369843415
661 661 a, c, t dbSNP:201876282
672 672 c, t dbSNP:763955290
680 680 c, t dbSNP:373020262
681 681 a, g dbSNP:779882018
685 685 c, t dbSNP:375988717
686 686 a, g dbSNP:146531157
690 690 a, g dbSNP:544832101
691 691 c, g dbSNP:184524047
692 692 a, c, t dbSNP:578157654
693 693 c, t dbSNP:746273794
694 694 a, g dbSNP:769966432
695 695 a, g dbSNP:200924110
705 705 -, t dbSNP:751409607
705 705 a, g dbSNP:369095740
707 707 g, t dbSNP:550965943
708 708 c, g dbSNP:112142292
710 710 g, t dbSNP:774547744
715 715 a, g dbSNP:761633631
719 719 c, t dbSNP:61745373
724 724 c, t dbSNP:767528406
725 725 c, t dbSNP:750201200
726 726 -, a dbSNP:757539569
726 726 c, t dbSNP:760566709
731 731 c, g dbSNP:766069044
738 738 a, c dbSNP:753541240
751 751 a, c dbSNP:371204759
752 752 a, g dbSNP:778277201
756 756 c, t dbSNP:375457877
760 760 -, tgc dbSNP:781628496
760 760 c, t dbSNP:115331994
769 769 a, t dbSNP:368573599
772 772 c, g dbSNP:1997554
775 775 c, t dbSNP:267601038
778 778 a, g dbSNP:746183695
779 779 a, g dbSNP:370217795
781 781 a, g dbSNP:780183620
782 782 c, t dbSNP:768540118
798 798 c, t dbSNP:768971560
819 819 c, g dbSNP:774263017
834 834 a, g dbSNP:748288550
841 841 a, g dbSNP:372426467
845 845 c, t dbSNP:773293542
847 847 a, g dbSNP:61743125
854 854 g, t dbSNP:368137542
875 875 c, t dbSNP:776629714
876 876 a, g dbSNP:759092850
881 881 a, t dbSNP:764983525
883 883 -, tg dbSNP:750482976
918 918 -, c dbSNP:34259318
923 923 c, t dbSNP:562534073
951 951 a, g dbSNP:531362459
991 991 c, t dbSNP:528974068
992 992 g, t dbSNP:41274882

Target ORF information:

RefSeq Version NM_001243169
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243169

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
CCTGTTTCCT TCCCTTCCTC ACCAGAGGCT GCCACAACAG GCCCCATCTG GTTCTCAGCC
GGCAATGGCA GTGCACTGGA TGCCTTCACC TATGGCCCTT CCCCAGCAAC GGATGGCACC
TGGACCAACT TGGCCCATCT CTCCCTGCCT TCTGAGGAGC TGGCATCCTG GGAGCCTTTG
GTCTGCCACA CTGGGCCTGG GGCTGAGGGT CACAGCAGGA GTACACAGCC CATGCATCTG
TCAGGAGAGG CTTCTACAGC CAGGACCTGC CCCCAGGAGC CTCTCAGGGG GACACCGGGT
GGGGCGCTGT GGCTGGGGGT CCTGCGGCTG CTGCTCTTCA AGCTGCTGCT GTTTGACCTG
CTCCTGACCT GCAGCTGCCT GTGCGACCCC GCGGGCCCGC TGCCTTCCCC CGCAACCACC
ACCCGCCTGC GAGCCCTCGG CTCCCATCGA CTGCACCCGG CCACGGAGAC TGGGGGACGA
GAGGCCACCA GCTCACCCAG ACCCCAGCCT CGGGACCGCC GCTGGGGTGA CACCCCTCCG
GGTCGGAAGC CCGGGAGCCC AGTATGGGGG GAAGGGTCTT ACCTCAGCAG TTACCCCACT
TGCCCAGCAC AGGCCTGGTG CTCAAGATCT GCCCTCAGGG CTCCTTCCTC CAGTCTTGGA
GCATTTTTTG CAGGTGACCT GCCTCCTCCT CTGCAGGCTG GAGCTGCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29044
Accession Version NM_138296.2
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pre T-cell antigen receptor alpha isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U36759.1, BC100771.1, U38996.1 and AW014894.1. On Jul 7, 2006 this sequence version replaced gi:19923720. Summary: The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) lacks an alternate internal segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: U36759.1, BC100773.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGACAC CGGGTGGGGC GCTGTGGCTG GGGGTCCTGC GGCTGCTGCT CTTCAAGCTG
CTGCTGTTTG ACCTGCTCCT GACCTGCAGC TGCCTGTGCG ACCCCGCGGG CCCGCTGCCT
TCCCCCGCAA CCACCACCCG CCTGCGAGCC CTCGGCTCCC ATCGACTGCA CCCGGCCACG
GAGACTGGGG GACGAGAGGC CACCAGCTCA CCCAGACCCC AGCCTCGGGA CCGCCGCTGG
GGTGACACCC CTCCGGGTCG GAAGCCCGGG AGCCCAGTAT GGGGGGAAGG GTCTTACCTC
AGCAGTTACC CCACTTGCCC AGCACAGGCC TGGTGCTCAA GATCTGCCCT CAGGGCTCCT
TCCTCCAGTC TTGGAGCATT TTTTGCAGGT GACCTGCCTC CTCCTCTGCA GGCTGGAGCT
GCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_612153.2
CDS82..927
Misc Feature(1)1..3(+)
Misc Feature(2)139..600(+)
Exon (1)1..139
Gene:PTCRA
Gene Synonym:
Exon (2)140..460
Gene:PTCRA
Gene Synonym:
Exon (3)461..505
Gene:PTCRA
Gene Synonym:
Exon (4)506..1083
Gene:PTCRA
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:552097755
13 13 c, t dbSNP:559446349
25 25 c, t dbSNP:374451302
27 27 c, t dbSNP:200306211
32 32 c, g, t dbSNP:772667489
33 33 a, c, g dbSNP:761067985
34 34 c, g dbSNP:754225849
36 36 c, t dbSNP:548177244
37 37 a, c, g, t dbSNP:779867569
39 39 a, c dbSNP:777637876
40 40 a, g dbSNP:568097162
56 56 a, g dbSNP:756935540
57 57 a, g dbSNP:781036234
68 68 c, g dbSNP:561719796
79 79 a, g, t dbSNP:745700204
81 81 c, t dbSNP:779886058
87 87 c, t dbSNP:774492937
88 88 a, g dbSNP:376557082
93 93 -, a dbSNP:749643841
93 93 a, c, g dbSNP:138459529
96 96 g, t dbSNP:188435679
114 114 a, c dbSNP:747517503
129 129 c, t dbSNP:771396393
137 137 c, t dbSNP:777028518
139 139 c, g dbSNP:768315052
147 147 c, t dbSNP:530346250
148 148 a, g dbSNP:768821077
154 154 c, t dbSNP:750343059
156 156 c, g dbSNP:143960660
160 160 -, c dbSNP:760254872
160 160 c, t dbSNP:766274816
166 166 c, g dbSNP:753748329
169 169 g, t dbSNP:371971484
170 170 c, t dbSNP:778744161
176 176 c, g dbSNP:747651022
178 178 a, g dbSNP:199894504
188 188 g, t dbSNP:779277349
196 196 a, g dbSNP:757865292
199 199 a, g dbSNP:781681295
203 203 a, t dbSNP:746364033
206 206 a, t dbSNP:770351672
216 216 c, g dbSNP:780382863
219 219 c, t dbSNP:373111406
226 226 a, g, t dbSNP:768826241
228 228 c, t dbSNP:761896723
232 232 a, g dbSNP:772052468
233 233 a, c dbSNP:200942121
235 235 a, g dbSNP:760520383
241 241 c, g, t dbSNP:146960695
258 258 a, c, g dbSNP:372102699
261 261 c, t dbSNP:752303292
262 262 a, t dbSNP:757857439
276 276 c, t dbSNP:137970521
288 288 c, t dbSNP:751138873
289 289 c, g dbSNP:756591065
293 293 c, t dbSNP:780570974
294 294 a, g dbSNP:749605990
295 295 a, g dbSNP:769024317
300 300 c, t dbSNP:779125923
303 303 c, t dbSNP:748318011
308 308 a, g dbSNP:141630791
326 326 c, t dbSNP:773148448
327 327 a, g dbSNP:760873532
339 339 c, g dbSNP:770947767
349 349 a, g dbSNP:201708224
354 354 a, t dbSNP:759343944
364 364 c, t dbSNP:765126989
366 366 g, t dbSNP:752602270
376 376 c, g, t dbSNP:147986652
384 384 c, t dbSNP:750993939
391 391 c, g dbSNP:150508492
397 397 a, g dbSNP:9471966
405 405 c, t dbSNP:201816889
424 424 g, t dbSNP:754349321
425 425 g, t dbSNP:755548523
429 429 c, g dbSNP:200182239
431 431 a, g dbSNP:748599249
441 441 a, c dbSNP:758521719
445 445 c, t dbSNP:778083552
446 446 c, t dbSNP:747032918
451 451 c, t dbSNP:771146026
452 452 a, c, t dbSNP:141979329
475 475 c, g dbSNP:768503752
477 477 c, t dbSNP:548435231
481 481 a, g dbSNP:761444307
482 482 c, t dbSNP:767210145
502 502 a, g dbSNP:772682493
510 510 -, a dbSNP:763910876
512 512 c, t dbSNP:564467179
513 513 a, g dbSNP:753917737
514 514 a, c, g dbSNP:755076613
521 521 c, t dbSNP:752631531
522 522 a, g dbSNP:758393976
525 525 a, g dbSNP:533321958
527 527 a, g dbSNP:746826227
536 536 g, t dbSNP:756901784
541 541 c, t dbSNP:780737707
542 542 a, g, t dbSNP:745333497
562 562 c, t dbSNP:775207181
563 563 a, t dbSNP:547016440
573 573 c, t dbSNP:772757131
574 574 c, t dbSNP:773597378
587 587 a, g dbSNP:774026929
597 597 a, g dbSNP:760979735
600 600 c, g dbSNP:766577832
607 607 a, g dbSNP:776736708
608 608 a, c dbSNP:376897338
609 609 a, g dbSNP:765293082
614 614 c, t dbSNP:367893841
615 615 a, g dbSNP:184782115
627 627 c, t dbSNP:759156804
628 628 a, g dbSNP:36111725
634 634 a, c dbSNP:751330894
637 637 a, c dbSNP:757025642
640 640 c, t dbSNP:187988655
647 647 a, g dbSNP:111782749
655 655 a, g dbSNP:373866603
660 660 c, t dbSNP:779687830
661 661 c, t dbSNP:748845914
664 664 c, t dbSNP:542853935
665 665 c, g dbSNP:557469946
667 667 c, t dbSNP:747336644
668 668 c, t dbSNP:771200206
674 674 c, t dbSNP:571012981
675 675 a, g, t dbSNP:533516824
681 681 a, g dbSNP:775271650
694 694 c, t dbSNP:553747473
695 695 c, g dbSNP:775518759
702 702 c, t dbSNP:763053393
718 718 a, c dbSNP:764045039
727 727 c, t dbSNP:751466261
733 733 c, t dbSNP:200928800
734 734 a, g dbSNP:369843415
736 736 a, c, t dbSNP:201876282
747 747 c, t dbSNP:763955290
755 755 c, t dbSNP:373020262
756 756 a, g dbSNP:779882018
760 760 c, t dbSNP:375988717
761 761 a, g dbSNP:146531157
765 765 a, g dbSNP:544832101
766 766 c, g dbSNP:184524047
767 767 a, c, t dbSNP:578157654
768 768 c, t dbSNP:746273794
769 769 a, g dbSNP:769966432
770 770 a, g dbSNP:200924110
780 780 -, t dbSNP:751409607
780 780 a, g dbSNP:369095740
782 782 g, t dbSNP:550965943
783 783 c, g dbSNP:112142292
785 785 g, t dbSNP:774547744
790 790 a, g dbSNP:761633631
794 794 c, t dbSNP:61745373
799 799 c, t dbSNP:767528406
800 800 c, t dbSNP:750201200
801 801 -, a dbSNP:757539569
801 801 c, t dbSNP:760566709
806 806 c, g dbSNP:766069044
813 813 a, c dbSNP:753541240
826 826 a, c dbSNP:371204759
827 827 a, g dbSNP:778277201
831 831 c, t dbSNP:375457877
835 835 -, tgc dbSNP:781628496
835 835 c, t dbSNP:115331994
844 844 a, t dbSNP:368573599
847 847 c, g dbSNP:1997554
850 850 c, t dbSNP:267601038
853 853 a, g dbSNP:746183695
854 854 a, g dbSNP:370217795
856 856 a, g dbSNP:780183620
857 857 c, t dbSNP:768540118
873 873 c, t dbSNP:768971560
894 894 c, g dbSNP:774263017
909 909 a, g dbSNP:748288550
916 916 a, g dbSNP:372426467
920 920 c, t dbSNP:773293542
922 922 a, g dbSNP:61743125
929 929 g, t dbSNP:368137542
950 950 c, t dbSNP:776629714
951 951 a, g dbSNP:759092850
956 956 a, t dbSNP:764983525
958 958 -, tg dbSNP:750482976
993 993 -, c dbSNP:34259318
998 998 c, t dbSNP:562534073
1026 1026 a, g dbSNP:531362459
1066 1066 c, t dbSNP:528974068
1067 1067 g, t dbSNP:41274882

Target ORF information:

RefSeq Version NM_138296
Organism Homo sapiens (human)
Definition Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_138296

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGGTA CATGGCTGCT ACTTCTCCTG GCCCTTGGGT GTCCAGCCCT ACCCACAGGT 
GTGGGCGGCA CACCCTTTCC TTCTCTGGCC CCACCAATCA TGCTGCTGGT GGATGGAAAG
CAGCAGATGG TGGTGGTCTG CCTGGTCCTT GATGTTGCAC CCCCTGGCCT TGACAGCCCC
ATCTGGTTCT CAGCCGGCAA TGGCAGTGCA CTGGATGCCT TCACCTATGG CCCTTCCCCA
GCAACGGATG GCACCTGGAC CAACTTGGCC CATCTCTCCC TGCCTTCTGA GGAGCTGGCA
TCCTGGGAGC CTTTGGTCTG CCACACTGGG CCTGGGGCTG AGGGTCACAG CAGGAGTACA
CAGCCCATGC ATCTGTCAGG AGAGGCTTCT ACAGCCAGGA CCTGCCCCCA GGAGCCTCTC
AGGGGGACAC CGGGTGGGGC GCTGTGGCTG GGGGTCCTGC GGCTGCTGCT CTTCAAGCTG
CTGCTGTTTG ACCTGCTCCT GACCTGCAGC TGCCTGTGCG ACCCCGCGGG CCCGCTGCCT
TCCCCCGCAA CCACCACCCG CCTGCGAGCC CTCGGCTCCC ATCGACTGCA CCCGGCCACG
GAGACTGGGG GACGAGAGGC CACCAGCTCA CCCAGACCCC AGCCTCGGGA CCGCCGCTGG
GGTGACACCC CTCCGGGTCG GAAGCCCGGG AGCCCAGTAT GGGGGGAAGG GTCTTACCTC
AGCAGTTACC CCACTTGCCC AGCACAGGCC TGGTGCTCAA GATCTGCCCT CAGGGCTCCT
TCCTCCAGTC TTGGAGCATT TTTTGCAGGT GACCTGCCTC CTCCTCTGCA GGCTGGAGCT
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.