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DHCR7 7-dehydrocholesterol reductase [Homo sapiens (human)]

Gene Symbol DHCR7
Entrez Gene ID 1717
Full Name 7-dehydrocholesterol reductase
Synonyms SLOS
General protein information
Preferred Names
7-dehydrocholesterol reductase
Names
7-dehydrocholesterol reductase
7-DHC reductase
sterol delta-7-reductase
putative sterol reductase SR-2
delta-7-dehydrocholesterol reductase
NP_001157289.1
NP_001351.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13.4

Summary This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]. lac of sum
Disorder MIM:

602858

Disorder Html: Smith-Lemli-Opitz syndrome, 270400 (3)





The following DHCR7 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHCR7 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu57236 XM_011544777 PREDICTED: Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu21071 NM_001360 Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319
OHu21071 NM_001163817 Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu57236
Accession Version XM_011544777.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 7-dehydrocholesterol reductase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGAAGAGAA CACGGAGGCA AGGCGTGTGT CAGAGGCAGA GCTGGGGTTT GACCCCAGGC
CGCTGGGCCC CCGAGCCCAC ACTCCTGTCC TCTCCCTGGG CAAAGCACCG CTTGACCCCT
TCCCCCTCGC CCCCCAGGGT CTGTACTTGG TGTACCACCC CGTGCAGCTG TCCACCCCGC
ACGCCGTGGG CGTCCTGCTG CTGGGCCTGG TGGGCTACTA CATCTTCCGG GTGGCCAACC
ACCAGAAGGA CCTGTTCCGC CGCACGGATG GGCGCTGCCT CATCTGGGGC AGGAAGCCCA
AGGTCATCGA GTGCTCCTAC ACATCCGCCG ATGGGCAGAG GCACCACAGC AAGCTGCTGG
TGTCGGGCTT CTGGGGCGTG GCCCGCCACT TCAACTACGT CGGCGACCTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011543079.1
CDS224..1594
Misc Feature(1)500..>1192(+)
Translation
Position Chain Variation Link
17 17 g, t dbSNP:527301706
29 29 g, t dbSNP:141057811
174 174 a, g dbSNP:556280113
189 189 c, t dbSNP:75974711
190 190 g, t dbSNP:145915789
192 192 c, t dbSNP:762318777
201 201 c, t dbSNP:12573951
224 224 a, g dbSNP:104886033
226 226 a, g dbSNP:121909767
235 235 a, g dbSNP:757934834
237 237 c, g, t dbSNP:1127869
238 238 a, g dbSNP:147038941
241 241 a, g dbSNP:754333005
244 244 a, c dbSNP:766817989
246 246 a, g dbSNP:756194690
248 248 a, g, t dbSNP:115595829
251 251 c, g dbSNP:139166382
253 253 c, g dbSNP:775421040
261 261 a, g dbSNP:764925295
268 268 a, g dbSNP:150214075
274 274 c, t dbSNP:776713087
275 275 a, g dbSNP:770947907
277 277 c, t dbSNP:746536172
281 281 a, g dbSNP:772572550
283 283 -, a dbSNP:749174332
283 283 a, t dbSNP:771795285
292 292 c, t dbSNP:199798127
293 293 a, g, t dbSNP:146867923
294 294 a, c dbSNP:530687139
300 300 a, c dbSNP:199815542
302 302 a, g dbSNP:780570846
305 305 c, t dbSNP:756564881
307 307 a, g dbSNP:372855459
312 312 c, g dbSNP:200334114
314 314 a, c, t dbSNP:367585401
315 315 a, g dbSNP:370307688
318 318 c, t dbSNP:780180501
334 334 a, g dbSNP:750345068
341 341 c, t dbSNP:767425988
345 345 c, t dbSNP:761265690
346 346 a, g, t dbSNP:376263149
349 349 c, t dbSNP:150928869
350 350 a, g dbSNP:200984695
354 354 c, t dbSNP:142897396
360 360 g, t dbSNP:746175164
362 362 c, t dbSNP:140721259
370 370 c, t dbSNP:771419549
371 371 a, g dbSNP:747103823
372 372 a, c dbSNP:104886034
373 373 c, t dbSNP:540098203
374 374 c, t dbSNP:104886035
376 376 c, t dbSNP:777966196
379 379 c, g dbSNP:758757355
382 382 c, t dbSNP:752952700
383 383 a, g dbSNP:779222334
385 385 c, t dbSNP:755869989
388 388 c, g dbSNP:374941029
395 395 a, g, t dbSNP:104886032
399 399 g, t dbSNP:104886036
405 405 g, t dbSNP:761788547
408 408 a, t dbSNP:104886037
409 409 c, t dbSNP:763591043
412 412 a, g, t dbSNP:1044482
421 421 c, t dbSNP:775127532
422 422 a, g dbSNP:143999854
426 426 c, t dbSNP:104886038
430 430 c, t dbSNP:1790334
431 431 a, c, g dbSNP:144512551
433 433 c, t dbSNP:747544280
443 443 a, g dbSNP:773481689
444 444 a, c dbSNP:374874199
445 445 c, g, t dbSNP:183441430
446 446 a, t dbSNP:370748173
448 448 c, t dbSNP:755182484
449 449 a, g dbSNP:368473756
453 453 c, t dbSNP:781010025
454 454 c, t dbSNP:4316537
455 455 a, g dbSNP:373352413
464 464 c, g, t dbSNP:775735710
465 465 a, g dbSNP:752153214
471 471 c, t dbSNP:764847274
472 472 a, g dbSNP:759192320
474 474 a, g dbSNP:776923495
475 475 c, t dbSNP:766749461
488 488 a, t dbSNP:375997113
490 490 c, t dbSNP:760887459
491 491 c, t dbSNP:773862228
496 496 c, t dbSNP:772671149
501 501 c, t dbSNP:80338853
502 502 a, g dbSNP:774554996
505 505 a, g dbSNP:541593878
511 511 c, t dbSNP:745595242
512 512 a, g dbSNP:150563256
514 514 c, t dbSNP:780909138
515 515 c, t dbSNP:104886039
519 519 c, t dbSNP:104886041
526 526 c, t dbSNP:777709295
544 544 c, g dbSNP:104886040
547 547 a, g dbSNP:764443524
549 549 c, t dbSNP:121912195
550 550 c, t dbSNP:763112867
556 556 c, t dbSNP:775407178
558 558 c, t dbSNP:770040020
559 559 a, g dbSNP:759750125
565 565 a, c dbSNP:773047702
571 571 c, t dbSNP:373808237
578 578 -, c dbSNP:747827699
579 579 a, t dbSNP:28938174
581 581 a, g dbSNP:771866134
583 583 a, g dbSNP:201227366
592 592 c, g, t dbSNP:373452254
593 593 c, g dbSNP:748879289
598 598 c, t dbSNP:779401555
599 599 a, g dbSNP:143587828
607 607 c, t dbSNP:750090808
608 608 a, c, g dbSNP:138215017
609 609 c, t dbSNP:757703242
618 618 a, g dbSNP:751818759
619 619 a, g dbSNP:764707624
620 620 a, g dbSNP:763333510
622 622 c, t dbSNP:147424205
623 623 -, gtgactcc dbSNP:754560074
623 623 g, t dbSNP:201466849
631 631 g, t dbSNP:772940271
632 632 g, t dbSNP:563056315
638 638 a, g dbSNP:769816544
641 641 a, g dbSNP:373908315
654 654 a, g dbSNP:776246504
661 661 c, g, t dbSNP:949177
663 663 a, g dbSNP:777425801
665 665 c, t dbSNP:758693177
675 675 a, c, g dbSNP:11555217
676 676 a, g dbSNP:104894213
683 683 a, g dbSNP:779417085
684 684 c, g, t dbSNP:143312232
693 693 c, t dbSNP:753960624
694 694 c, g dbSNP:149164866
697 697 g, t dbSNP:766443353
698 698 c, t dbSNP:756416682
706 706 c, t dbSNP:750628746
707 707 a, g dbSNP:767716202
708 708 c, t dbSNP:398123606
711 711 a, g dbSNP:752494910
713 713 c, t dbSNP:774978807
715 715 c, g dbSNP:759538408
723 723 c, g dbSNP:776334336
729 729 c, g, t dbSNP:80338855
730 730 a, g dbSNP:138185442
732 732 c, g dbSNP:150459687
736 736 c, t dbSNP:566499249
737 737 a, g dbSNP:748562900
740 740 a, t dbSNP:779219548
744 744 c, t dbSNP:769218623
745 745 c, t dbSNP:140648594
746 746 a, g dbSNP:368269558
761 761 -, cac dbSNP:779528392
766 766 c, g dbSNP:146839126
768 768 a, g dbSNP:536394774
772 772 c, t dbSNP:115446684
781 781 c, t dbSNP:757519627
792 792 c, t dbSNP:752603560
793 793 c, t dbSNP:74909468
794 794 a, g dbSNP:532635888
797 797 c, t dbSNP:753830704
798 798 c, t dbSNP:766299724
799 799 c, t dbSNP:760123718
802 802 c, g dbSNP:778664009
805 805 c, t dbSNP:150603941
806 806 a, g dbSNP:151170252
809 809 a, g dbSNP:200474791
810 810 c, t dbSNP:142138656
824 824 c, t dbSNP:774755673
826 826 a, c dbSNP:377727130
830 830 c, t dbSNP:749591513
838 838 c, t dbSNP:561379509
839 839 a, g dbSNP:542744675
847 847 c, t dbSNP:374640274
861 861 a, g dbSNP:769583377
868 868 a, c dbSNP:773048017
874 874 a, c dbSNP:749076525
878 878 g, t dbSNP:779896782
880 880 c, t dbSNP:376031781
881 881 a, c, g dbSNP:200659923
883 883 g, t dbSNP:767377692
884 884 a, g dbSNP:756795175
888 888 a, g dbSNP:751026224
889 889 c, t dbSNP:763787299
893 893 a, g dbSNP:373121544
905 905 c, t dbSNP:775773057
906 906 a, g dbSNP:201556114
910 910 a, c, g, t dbSNP:267603172
911 911 a, g dbSNP:747133004
913 913 c, g dbSNP:773393807
915 915 a, g dbSNP:772491341
917 917 c, t dbSNP:748390752
928 928 c, t dbSNP:778945317
932 932 c, t dbSNP:755941916
942 942 a, g dbSNP:148609143
946 946 g, t dbSNP:369654651
947 947 c, t dbSNP:80338856
948 948 a, g dbSNP:80338857
951 951 c, g dbSNP:777248132
952 952 c, t dbSNP:145284180
953 953 a, g dbSNP:121909764
958 958 c, t dbSNP:12800
959 959 a, g dbSNP:759967245
961 961 c, t dbSNP:536834609
962 962 a, g dbSNP:767031102
964 964 c, g, t dbSNP:773485715
967 967 g, t dbSNP:104894212
971 971 c, g dbSNP:772294537
974 974 a, g dbSNP:761732160
979 979 c, t dbSNP:774666247
985 985 c, g, t dbSNP:113950278
988 988 c, t dbSNP:200132007
989 989 a, g, t dbSNP:772639348
993 993 c, t dbSNP:770925697
994 994 a, g dbSNP:200232012
1001 1001 c, g dbSNP:777617141
1002 1002 a, g dbSNP:551260416
1004 1004 a, g dbSNP:752096930
1006 1006 a, g dbSNP:778674271
1007 1007 c, g dbSNP:754553051
1012 1012 c, t dbSNP:754320411
1013 1013 a, g dbSNP:766838675
1026 1026 a, g dbSNP:761064004
1027 1027 g, t dbSNP:750973099
1037 1037 c, t dbSNP:767872725
1039 1039 a, g dbSNP:761978602
1043 1043 -, aacgtc dbSNP:757697462
1044 1044 a, c dbSNP:774275482
1045 1045 a, c, t dbSNP:139787408
1046 1046 a, g dbSNP:775628929
1051 1051 a, g dbSNP:770713196
1058 1058 a, g dbSNP:752010614
1062 1062 a, g dbSNP:121909766
1063 1063 c, t dbSNP:148468879
1064 1064 a, g dbSNP:398123607
1075 1075 a, c dbSNP:184297154
1078 1078 c, t dbSNP:781026169
1083 1083 a, g dbSNP:765481717
1084 1084 a, c, t dbSNP:766495775
1085 1085 a, g dbSNP:565893436
1089 1089 c, t dbSNP:121909765
1090 1090 c, t dbSNP:761602799
1093 1093 a, g dbSNP:774187452
1097 1097 c, t dbSNP:768668145
1105 1105 c, t dbSNP:748858240
1106 1106 a, g dbSNP:201574502
1117 1117 c, t dbSNP:769639753
1118 1118 c, t dbSNP:745498103
1129 1129 c, g, t dbSNP:80338858
1130 1130 a, g, t dbSNP:142808899
1150 1150 a, c, t dbSNP:149974099
1151 1151 a, g dbSNP:370955781
1170 1170 a, g dbSNP:754992933
1179 1179 c, g, t dbSNP:766583874
1180 1180 a, g, t dbSNP:200157761
1196 1196 c, t dbSNP:143811340
1197 1197 a, g dbSNP:191941044
1201 1201 g, t dbSNP:756330549
1207 1207 c, t dbSNP:561898240
1208 1208 a, g dbSNP:763970199
1212 1212 a, g dbSNP:762782615
1215 1215 a, c dbSNP:752689386
1225 1225 a, c dbSNP:542781733
1242 1242 c, g dbSNP:759013440
1244 1244 c, t dbSNP:529912535
1246 1246 -, c dbSNP:754273826
1250 1250 c, g dbSNP:770554122
1254 1254 c, t dbSNP:1792268
1255 1255 c, g, t dbSNP:769884600
1256 1256 a, c, g dbSNP:375658179
1266 1266 c, t dbSNP:774861874
1268 1268 -, c dbSNP:764453914
1272 1272 a, c dbSNP:769186258
1273 1273 c, g dbSNP:749369790
1278 1278 c, t dbSNP:372086592
1280 1280 -, t dbSNP:756419639
1291 1291 c, g dbSNP:780180189
1292 1292 c, t dbSNP:530212349
1293 1293 a, g dbSNP:746110554
1299 1299 a, c dbSNP:781503283
1300 1300 c, g dbSNP:758285967
1301 1301 -, accc dbSNP:752946422
1305 1305 c, g dbSNP:752515369
1308 1308 c, t dbSNP:765302789
1310 1310 g, t dbSNP:577213881
1311 1311 c, t dbSNP:753345689
1312 1312 a, g dbSNP:765926518
1313 1313 c, t dbSNP:760035984
1315 1315 c, t dbSNP:772882845
1316 1316 c, t dbSNP:767108226
1317 1317 c, t dbSNP:762215750
1320 1320 c, g, t dbSNP:138659167
1333 1333 g, t dbSNP:80338859
1339 1339 c, g dbSNP:775805851
1344 1344 c, t dbSNP:145901607
1345 1345 a, c, g dbSNP:139724817
1357 1357 a, g dbSNP:757518371
1358 1358 c, t dbSNP:748020179
1359 1359 a, c dbSNP:778753729
1361 1361 c, g, t dbSNP:77762671
1362 1362 a, g dbSNP:768765843
1365 1365 c, t dbSNP:75225632
1366 1366 a, g dbSNP:749913821
1368 1368 c, t dbSNP:369382960
1369 1369 a, g dbSNP:72954276
1374 1374 c, t dbSNP:774757811
1375 1375 a, g dbSNP:148081697
1377 1377 c, t dbSNP:763165078
1382 1382 c, t dbSNP:199957106
1392 1392 c, g dbSNP:776099806
1397 1397 g, t dbSNP:770174208
1399 1399 -, ctggtgggct dbSNP:767602487
1409 1409 c, t dbSNP:779980243
1411 1411 c, t dbSNP:80338860
1412 1412 a, g, t dbSNP:121909768
1414 1414 -, g dbSNP:759720450
1416 1416 a, g dbSNP:747245607
1417 1417 a, g dbSNP:571923299
1419 1419 c, g dbSNP:145651408
1423 1423 -, c dbSNP:774291653
1428 1428 g, t dbSNP:768526200
1431 1431 a, g dbSNP:748986631
1440 1440 a, c dbSNP:780088227
1441 1441 c, t dbSNP:371302153
1442 1442 a, g, t dbSNP:142213147
1444 1444 c, t dbSNP:547012639
1445 1445 a, g dbSNP:200539324
1448 1448 c, t dbSNP:567600444
1449 1449 a, g dbSNP:35946774
1456 1456 c, t dbSNP:531038145
1457 1457 a, g dbSNP:765908713
1471 1471 g, t dbSNP:760104729
1483 1483 a, g dbSNP:777218262
1484 1484 a, g dbSNP:771137757
1491 1491 c, g, t dbSNP:773505265
1492 1492 a, g dbSNP:772162793
1495 1495 c, t dbSNP:373306653
1496 1496 a, g, t dbSNP:779709646
1497 1497 a, c dbSNP:769783115
1505 1505 c, t dbSNP:745820609
1508 1508 c, g dbSNP:781043327
1509 1509 c, t dbSNP:145084285
1510 1510 a, g dbSNP:750989470
1511 1511 c, g dbSNP:12577137
1512 1512 c, t dbSNP:777517985
1513 1513 a, g dbSNP:369837196
1515 1515 a, c, t dbSNP:760241
1516 1516 a, g dbSNP:765707139
1523 1523 a, g dbSNP:755325060
1525 1525 c, t dbSNP:544442568
1526 1526 a, g dbSNP:766943022
1544 1544 a, t dbSNP:760857590
1547 1547 c, t dbSNP:773134475
1548 1548 a, g dbSNP:767847651
1555 1555 g, t dbSNP:78523425
1560 1560 c, t dbSNP:368867929
1561 1561 a, c, g dbSNP:200099137
1567 1567 c, t dbSNP:61757582
1572 1572 c, g dbSNP:776577137
1576 1576 a, t dbSNP:770819693
1577 1577 a, g dbSNP:746591926
1581 1581 c, t dbSNP:777148033
1582 1582 a, g dbSNP:757861528
1585 1585 a, g dbSNP:80338862
1588 1588 a, g dbSNP:372055524
1597 1597 c, g dbSNP:755426995
1600 1600 a, g dbSNP:754224400
1608 1608 c, t dbSNP:767043016
1617 1617 a, g dbSNP:756564409
1621 1621 c, t dbSNP:750582708
1626 1626 c, t dbSNP:145374203
1627 1627 a, g, t dbSNP:368150818
1629 1629 c, t dbSNP:909217
1630 1630 a, g dbSNP:760242
1633 1633 c, t dbSNP:776401706
1635 1635 a, c dbSNP:770971876
1641 1641 a, g dbSNP:746928218
1647 1647 c, g dbSNP:140791666
1650 1650 c, t dbSNP:771506862
1651 1651 c, t dbSNP:747656720
1657 1657 a, g dbSNP:375187933
1671 1671 c, g dbSNP:778750039
1677 1677 a, g dbSNP:754655629
1678 1678 a, t dbSNP:749728888
1684 1684 c, t dbSNP:535561852
1685 1685 a, c, g, t dbSNP:781687341
1693 1693 -, tcc dbSNP:766416330
1693 1693 c, t dbSNP:145043679
1694 1694 a, g dbSNP:751604696
1695 1695 c, g dbSNP:201716149
1698 1698 c, g, t dbSNP:139721775
1699 1699 a, g dbSNP:80338864
1702 1702 c, t dbSNP:758040709
1705 1705 c, t dbSNP:765154144
1706 1706 a, g, t dbSNP:542266962
1707 1707 c, g dbSNP:772016342
1708 1708 c, t dbSNP:761458977
1711 1711 a, g dbSNP:140400648
1715 1715 c, g dbSNP:768489010
1719 1719 a, g dbSNP:147850435
1722 1722 c, t dbSNP:557097410
1723 1723 a, g dbSNP:201847193
1724 1724 g, t dbSNP:746201510
1725 1725 c, g, t dbSNP:144562471
1726 1726 c, t dbSNP:371873032
1736 1736 a, g dbSNP:777838196
1738 1738 c, t dbSNP:199506852
1741 1741 c, t dbSNP:201270451
1746 1746 c, t dbSNP:200477386
1747 1747 a, g dbSNP:566784842
1749 1749 c, t dbSNP:368281869
1753 1753 a, g dbSNP:760428437
1756 1756 a, c dbSNP:750211727
1762 1762 c, t dbSNP:148660993
1763 1763 a, c, g dbSNP:201150384
1767 1767 a, g dbSNP:375993195
1769 1769 c, t dbSNP:766709599
1783 1783 c, t dbSNP:775034584
1788 1788 a, g dbSNP:769454033
1789 1789 c, t dbSNP:371307569
1790 1790 -, ggca dbSNP:773237938
1792 1792 a, g dbSNP:537515766
1794 1794 c, t dbSNP:570180636
1805 1805 g, t dbSNP:747532529
1806 1806 c, t dbSNP:753568521
1810 1810 a, g dbSNP:778207094
1813 1813 a, g, t dbSNP:114143715
1815 1815 a, g, t dbSNP:755215281
1815 1815 -, g dbSNP:769745063
1826 1826 c, t dbSNP:750890505
1827 1827 a, g dbSNP:201871263
1835 1835 c, t dbSNP:757059514
1842 1842 c, t dbSNP:182965373
1849 1849 c, g dbSNP:546830267
1868 1868 c, t dbSNP:11555218
1870 1870 c, t dbSNP:528375215
1904 1904 a, g dbSNP:151230950
1905 1905 a, t dbSNP:542228378
1916 1916 c, t dbSNP:143459841
1934 1934 c, t dbSNP:751563187
1942 1942 -, cgctctgttcct dbSNP:373023739
1943 1943 -, gctctgttcctt dbSNP:368755383
1943 1943 a, g dbSNP:563020897
1946 1946 -, ctgttccttgct dbSNP:141483210
1946 1946 c, t dbSNP:200229535
1957 1957 -, ctgttccttgct dbSNP:753444031
1959 1959 c, t dbSNP:762590224
1962 1962 c, t dbSNP:775040338
1963 1963 a, c dbSNP:764851572
1967 1967 a, t dbSNP:759235974
1977 1977 c, t dbSNP:115338563
1978 1978 a, g dbSNP:771310150
1980 1980 a, g dbSNP:578117764
1981 1981 c, t dbSNP:747336124
1985 1985 c, t dbSNP:113188310
2011 2011 c, t dbSNP:185557595
2059 2059 c, t dbSNP:748234524
2089 2089 g, t dbSNP:1802125
2109 2109 c, t dbSNP:759058043
2118 2118 -, ct dbSNP:763034353
2143 2143 c, t dbSNP:78575838
2188 2188 a, c dbSNP:550781403
2201 2201 a, g dbSNP:180885292
2202 2202 -, a dbSNP:34995793
2202 2202 c, t dbSNP:537429160
2206 2206 c, g dbSNP:570096062
2207 2207 a, g dbSNP:529448827
2236 2236 a, g dbSNP:11233662
2265 2265 c, t dbSNP:1790345
2286 2286 a, g dbSNP:370708079
2308 2308 a, g dbSNP:546497674
2337 2337 g, t dbSNP:528351472
2350 2350 a, g dbSNP:79320071
2398 2398 a, g dbSNP:548893003
2425 2425 a, c dbSNP:530422056
2428 2428 c, t dbSNP:1044535
2434 2434 g, t dbSNP:368948615
2450 2450 a, g dbSNP:576060258
2474 2474 -, c dbSNP:34258235
2519 2519 a, c, g, t dbSNP:7690
2527 2527 g, t dbSNP:1802378
2531 2531 a, g dbSNP:564337568
2540 2540 c, t dbSNP:58046295
2541 2541 a, g dbSNP:546098047
2549 2549 c, t dbSNP:559871324
2550 2550 a, g dbSNP:777137931
2564 2564 c, t dbSNP:541282653
2568 2568 a, g dbSNP:747769421
2575 2575 c, t dbSNP:12407
2591 2591 a, g dbSNP:374355489
2612 2612 a, t dbSNP:146774511
2614 2614 a, g dbSNP:190840764
2674 2674 a, g dbSNP:185936568
2724 2724 a, g dbSNP:143699520
2726 2726 a, g dbSNP:576498440
2745 2745 a, t dbSNP:758713975

Target ORF information:

RefSeq Version XM_011544777
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544777

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGAAGAGAA CACGGAGGCA AGGCGTGTGT CAGAGGCAGA GCTGGGGTTT GACCCCAGGC
CGCTGGGCCC CCGAGCCCAC ACTCCTGTCC TCTCCCTGGG CAAAGCACCG CTTGACCCCT
TCCCCCTCGC CCCCCAGGGT CTGTACTTGG TGTACCACCC CGTGCAGCTG TCCACCCCGC
ACGCCGTGGG CGTCCTGCTG CTGGGCCTGG TGGGCTACTA CATCTTCCGG GTGGCCAACC
ACCAGAAGGA CCTGTTCCGC CGCACGGATG GGCGCTGCCT CATCTGGGGC AGGAAGCCCA
AGGTCATCGA GTGCTCCTAC ACATCCGCCG ATGGGCAGAG GCACCACAGC AAGCTGCTGG
TGTCGGGCTT CTGGGGCGTG GCCCGCCACT TCAACTACGT CGGCGACCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21071
Accession Version NM_001360.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 7-dehydrocholesterol reductase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA502590.1, BU848891.1, AF062481.1 and AP002387.4. This sequence is a reference standard in the RefSeqGene project. On Dec 25, 2006 this sequence version replaced gi:4503320. Summary: This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF034544.1, AK289497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001351.2
CDS274..1701
Misc Feature(1)193..195(+)
Misc Feature(2)313..315(+)
Misc Feature(3)391..453(+)
Misc Feature(4)550..1698(+)
Misc Feature(5)733..795(+)
Misc Feature(6)802..864(+)
Misc Feature(7)1069..1131(+)
Misc Feature(8)1189..1251(+)
Misc Feature(9)1264..1326(+)
Misc Feature(10)1531..1593(+)
Exon (1)1..142
Gene:DHCR7
Gene Synonym:
Exon (2)143..267
Gene:DHCR7
Gene Synonym:
Exon (3)268..371
Gene:DHCR7
Gene Synonym:
Exon (4)372..594
Gene:DHCR7
Gene Synonym:
Exon (5)595..685
Gene:DHCR7
Gene Synonym:
Exon (6)686..899
Gene:DHCR7
Gene Synonym:
Exon (7)900..1104
Gene:DHCR7
Gene Synonym:
Exon (8)1105..1236
Gene:DHCR7
Gene Synonym:
Exon (9)1237..2665
Gene:DHCR7
Gene Synonym:
Translation
Position Chain Variation Link
49 49 c, g, t dbSNP:4944946
67 67 g, t dbSNP:527301706
79 79 g, t dbSNP:141057811
224 224 a, g dbSNP:556280113
239 239 c, t dbSNP:75974711
240 240 g, t dbSNP:145915789
242 242 c, t dbSNP:762318777
251 251 c, t dbSNP:12573951
274 274 a, g dbSNP:104886033
276 276 a, g dbSNP:121909767
285 285 a, g dbSNP:757934834
287 287 c, g, t dbSNP:1127869
288 288 a, g dbSNP:147038941
291 291 a, g dbSNP:754333005
294 294 a, c dbSNP:766817989
296 296 a, g dbSNP:756194690
298 298 a, g, t dbSNP:115595829
301 301 c, g dbSNP:139166382
303 303 c, g dbSNP:775421040
311 311 a, g dbSNP:764925295
318 318 a, g dbSNP:150214075
324 324 c, t dbSNP:776713087
325 325 a, g dbSNP:770947907
327 327 c, t dbSNP:746536172
331 331 a, g dbSNP:772572550
333 333 -, a dbSNP:749174332
333 333 a, t dbSNP:771795285
342 342 c, t dbSNP:199798127
343 343 a, g, t dbSNP:146867923
344 344 a, c dbSNP:530687139
350 350 a, c dbSNP:199815542
352 352 a, g dbSNP:780570846
355 355 c, t dbSNP:756564881
357 357 a, g dbSNP:372855459
362 362 c, g dbSNP:200334114
364 364 a, c, t dbSNP:367585401
365 365 a, g dbSNP:370307688
368 368 c, t dbSNP:780180501
384 384 a, g dbSNP:750345068
391 391 c, t dbSNP:767425988
395 395 c, t dbSNP:761265690
396 396 a, g, t dbSNP:376263149
399 399 c, t dbSNP:150928869
400 400 a, g dbSNP:200984695
404 404 c, t dbSNP:142897396
410 410 g, t dbSNP:746175164
412 412 c, t dbSNP:140721259
420 420 c, t dbSNP:771419549
421 421 a, g dbSNP:747103823
422 422 a, c dbSNP:104886034
423 423 c, t dbSNP:540098203
424 424 c, t dbSNP:104886035
426 426 c, t dbSNP:777966196
429 429 c, g dbSNP:758757355
432 432 c, t dbSNP:752952700
433 433 a, g dbSNP:779222334
435 435 c, t dbSNP:755869989
438 438 c, g dbSNP:374941029
445 445 a, g, t dbSNP:104886032
449 449 g, t dbSNP:104886036
455 455 g, t dbSNP:761788547
458 458 a, t dbSNP:104886037
459 459 c, t dbSNP:763591043
462 462 a, g, t dbSNP:1044482
471 471 c, t dbSNP:775127532
472 472 a, g dbSNP:143999854
476 476 c, t dbSNP:104886038
480 480 c, t dbSNP:1790334
481 481 a, c, g dbSNP:144512551
483 483 c, t dbSNP:747544280
493 493 a, g dbSNP:773481689
494 494 a, c dbSNP:374874199
495 495 c, g, t dbSNP:183441430
496 496 a, t dbSNP:370748173
498 498 c, t dbSNP:755182484
499 499 a, g dbSNP:368473756
503 503 c, t dbSNP:781010025
504 504 c, t dbSNP:4316537
505 505 a, g dbSNP:373352413
514 514 c, g, t dbSNP:775735710
515 515 a, g dbSNP:752153214
521 521 c, t dbSNP:764847274
522 522 a, g dbSNP:759192320
524 524 a, g dbSNP:776923495
525 525 c, t dbSNP:766749461
538 538 a, t dbSNP:375997113
540 540 c, t dbSNP:760887459
541 541 c, t dbSNP:773862228
546 546 c, t dbSNP:772671149
551 551 c, t dbSNP:80338853
552 552 a, g dbSNP:774554996
555 555 a, g dbSNP:541593878
561 561 c, t dbSNP:745595242
562 562 a, g dbSNP:150563256
564 564 c, t dbSNP:780909138
565 565 c, t dbSNP:104886039
569 569 c, t dbSNP:104886041
576 576 c, t dbSNP:777709295
594 594 c, g dbSNP:104886040
597 597 a, g dbSNP:764443524
599 599 c, t dbSNP:121912195
600 600 c, t dbSNP:763112867
606 606 c, t dbSNP:775407178
608 608 c, t dbSNP:770040020
609 609 a, g dbSNP:759750125
615 615 a, c dbSNP:773047702
621 621 c, t dbSNP:373808237
628 628 -, c dbSNP:747827699
629 629 a, t dbSNP:28938174
631 631 a, g dbSNP:771866134
633 633 a, g dbSNP:201227366
642 642 c, g, t dbSNP:373452254
643 643 c, g dbSNP:748879289
648 648 c, t dbSNP:779401555
649 649 a, g dbSNP:143587828
657 657 c, t dbSNP:750090808
658 658 a, c, g dbSNP:138215017
659 659 c, t dbSNP:757703242
668 668 a, g dbSNP:751818759
669 669 a, g dbSNP:764707624
670 670 a, g dbSNP:763333510
672 672 c, t dbSNP:147424205
673 673 -, gtgactcc dbSNP:754560074
673 673 g, t dbSNP:201466849
681 681 g, t dbSNP:772940271
682 682 g, t dbSNP:563056315
688 688 a, g dbSNP:769816544
691 691 a, g dbSNP:373908315
704 704 a, g dbSNP:776246504
711 711 c, g, t dbSNP:949177
713 713 a, g dbSNP:777425801
715 715 c, t dbSNP:758693177
725 725 a, c, g dbSNP:11555217
726 726 a, g dbSNP:104894213
733 733 a, g dbSNP:779417085
734 734 c, g, t dbSNP:143312232
743 743 c, t dbSNP:753960624
744 744 c, g dbSNP:149164866
747 747 g, t dbSNP:766443353
748 748 c, t dbSNP:756416682
756 756 c, t dbSNP:750628746
757 757 a, g dbSNP:767716202
758 758 c, t dbSNP:398123606
761 761 a, g dbSNP:752494910
763 763 c, t dbSNP:774978807
765 765 c, g dbSNP:759538408
773 773 c, g dbSNP:776334336
779 779 c, g, t dbSNP:80338855
780 780 a, g dbSNP:138185442
782 782 c, g dbSNP:150459687
786 786 c, t dbSNP:566499249
787 787 a, g dbSNP:748562900
790 790 a, t dbSNP:779219548
794 794 c, t dbSNP:769218623
795 795 c, t dbSNP:140648594
796 796 a, g dbSNP:368269558
811 811 -, cac dbSNP:779528392
816 816 c, g dbSNP:146839126
818 818 a, g dbSNP:536394774
822 822 c, t dbSNP:115446684
831 831 c, t dbSNP:757519627
842 842 c, t dbSNP:752603560
843 843 c, t dbSNP:74909468
844 844 a, g dbSNP:532635888
847 847 c, t dbSNP:753830704
848 848 c, t dbSNP:766299724
849 849 c, t dbSNP:760123718
852 852 c, g dbSNP:778664009
855 855 c, t dbSNP:150603941
856 856 a, g dbSNP:151170252
859 859 a, g dbSNP:200474791
860 860 c, t dbSNP:142138656
874 874 c, t dbSNP:774755673
876 876 a, c dbSNP:377727130
880 880 c, t dbSNP:749591513
888 888 c, t dbSNP:561379509
889 889 a, g dbSNP:542744675
897 897 c, t dbSNP:374640274
911 911 a, g dbSNP:769583377
918 918 a, c dbSNP:773048017
924 924 a, c dbSNP:749076525
928 928 g, t dbSNP:779896782
930 930 c, t dbSNP:376031781
931 931 a, c, g dbSNP:200659923
933 933 g, t dbSNP:767377692
934 934 a, g dbSNP:756795175
938 938 a, g dbSNP:751026224
939 939 c, t dbSNP:763787299
943 943 a, g dbSNP:373121544
955 955 c, t dbSNP:775773057
956 956 a, g dbSNP:201556114
960 960 a, c, g, t dbSNP:267603172
961 961 a, g dbSNP:747133004
963 963 c, g dbSNP:773393807
965 965 a, g dbSNP:772491341
967 967 c, t dbSNP:748390752
978 978 c, t dbSNP:778945317
982 982 c, t dbSNP:755941916
992 992 a, g dbSNP:148609143
996 996 g, t dbSNP:369654651
997 997 c, t dbSNP:80338856
998 998 a, g dbSNP:80338857
1001 1001 c, g dbSNP:777248132
1002 1002 c, t dbSNP:145284180
1003 1003 a, g dbSNP:121909764
1008 1008 c, t dbSNP:12800
1009 1009 a, g dbSNP:759967245
1011 1011 c, t dbSNP:536834609
1012 1012 a, g dbSNP:767031102
1014 1014 c, g, t dbSNP:773485715
1017 1017 g, t dbSNP:104894212
1021 1021 c, g dbSNP:772294537
1024 1024 a, g dbSNP:761732160
1029 1029 c, t dbSNP:774666247
1035 1035 c, g, t dbSNP:113950278
1038 1038 c, t dbSNP:200132007
1039 1039 a, g, t dbSNP:772639348
1043 1043 c, t dbSNP:770925697
1044 1044 a, g dbSNP:200232012
1051 1051 c, g dbSNP:777617141
1052 1052 a, g dbSNP:551260416
1054 1054 a, g dbSNP:752096930
1056 1056 a, g dbSNP:778674271
1057 1057 c, g dbSNP:754553051
1062 1062 c, t dbSNP:754320411
1063 1063 a, g dbSNP:766838675
1076 1076 a, g dbSNP:761064004
1077 1077 g, t dbSNP:750973099
1087 1087 c, t dbSNP:767872725
1089 1089 a, g dbSNP:761978602
1093 1093 -, aacgtc dbSNP:757697462
1094 1094 a, c dbSNP:774275482
1095 1095 a, c, t dbSNP:139787408
1096 1096 a, g dbSNP:775628929
1101 1101 a, g dbSNP:770713196
1108 1108 a, g dbSNP:752010614
1112 1112 a, g dbSNP:121909766
1113 1113 c, t dbSNP:148468879
1114 1114 a, g dbSNP:398123607
1125 1125 a, c dbSNP:184297154
1128 1128 c, t dbSNP:781026169
1133 1133 a, g dbSNP:765481717
1134 1134 a, c, t dbSNP:766495775
1135 1135 a, g dbSNP:565893436
1139 1139 c, t dbSNP:121909765
1140 1140 c, t dbSNP:761602799
1143 1143 a, g dbSNP:774187452
1147 1147 c, t dbSNP:768668145
1155 1155 c, t dbSNP:748858240
1156 1156 a, g dbSNP:201574502
1167 1167 c, t dbSNP:769639753
1168 1168 c, t dbSNP:745498103
1179 1179 c, g, t dbSNP:80338858
1180 1180 a, g, t dbSNP:142808899
1200 1200 a, c, t dbSNP:149974099
1201 1201 a, g dbSNP:370955781
1220 1220 a, g dbSNP:754992933
1229 1229 c, g, t dbSNP:766583874
1230 1230 a, g, t dbSNP:200157761
1249 1249 g, t dbSNP:80338859
1255 1255 c, g dbSNP:775805851
1260 1260 c, t dbSNP:145901607
1261 1261 a, c, g dbSNP:139724817
1273 1273 a, g dbSNP:757518371
1274 1274 c, t dbSNP:748020179
1275 1275 a, c dbSNP:778753729
1277 1277 c, g, t dbSNP:77762671
1278 1278 a, g dbSNP:768765843
1281 1281 c, t dbSNP:75225632
1282 1282 a, g dbSNP:749913821
1284 1284 c, t dbSNP:369382960
1285 1285 a, g dbSNP:72954276
1290 1290 c, t dbSNP:774757811
1291 1291 a, g dbSNP:148081697
1293 1293 c, t dbSNP:763165078
1298 1298 c, t dbSNP:199957106
1308 1308 c, g dbSNP:776099806
1313 1313 g, t dbSNP:770174208
1315 1315 -, ctggtgggct dbSNP:767602487
1325 1325 c, t dbSNP:779980243
1327 1327 c, t dbSNP:80338860
1328 1328 a, g, t dbSNP:121909768
1330 1330 -, g dbSNP:759720450
1332 1332 a, g dbSNP:747245607
1333 1333 a, g dbSNP:571923299
1335 1335 c, g dbSNP:145651408
1339 1339 -, c dbSNP:774291653
1344 1344 g, t dbSNP:768526200
1347 1347 a, g dbSNP:748986631
1356 1356 a, c dbSNP:780088227
1357 1357 c, t dbSNP:371302153
1358 1358 a, g, t dbSNP:142213147
1360 1360 c, t dbSNP:547012639
1361 1361 a, g dbSNP:200539324
1364 1364 c, t dbSNP:567600444
1365 1365 a, g dbSNP:35946774
1372 1372 c, t dbSNP:531038145
1373 1373 a, g dbSNP:765908713
1387 1387 g, t dbSNP:760104729
1399 1399 a, g dbSNP:777218262
1400 1400 a, g dbSNP:771137757
1407 1407 c, g, t dbSNP:773505265
1408 1408 a, g dbSNP:772162793
1411 1411 c, t dbSNP:373306653
1412 1412 a, g, t dbSNP:779709646
1413 1413 a, c dbSNP:769783115
1421 1421 c, t dbSNP:745820609
1424 1424 c, g dbSNP:781043327
1425 1425 c, t dbSNP:145084285
1426 1426 a, g dbSNP:750989470
1427 1427 c, g dbSNP:12577137
1428 1428 c, t dbSNP:777517985
1429 1429 a, g dbSNP:369837196
1431 1431 a, c, t dbSNP:760241
1432 1432 a, g dbSNP:765707139
1439 1439 a, g dbSNP:755325060
1441 1441 c, t dbSNP:544442568
1442 1442 a, g dbSNP:766943022
1460 1460 a, t dbSNP:760857590
1463 1463 c, t dbSNP:773134475
1464 1464 a, g dbSNP:767847651
1471 1471 g, t dbSNP:78523425
1476 1476 c, t dbSNP:368867929
1477 1477 a, c, g dbSNP:200099137
1483 1483 c, t dbSNP:61757582
1488 1488 c, g dbSNP:776577137
1492 1492 a, t dbSNP:770819693
1493 1493 a, g dbSNP:746591926
1497 1497 c, t dbSNP:777148033
1498 1498 a, g dbSNP:757861528
1501 1501 a, g dbSNP:80338862
1504 1504 a, g dbSNP:372055524
1513 1513 c, g dbSNP:755426995
1516 1516 a, g dbSNP:754224400
1524 1524 c, t dbSNP:767043016
1533 1533 a, g dbSNP:756564409
1537 1537 c, t dbSNP:750582708
1542 1542 c, t dbSNP:145374203
1543 1543 a, g, t dbSNP:368150818
1545 1545 c, t dbSNP:909217
1546 1546 a, g dbSNP:760242
1549 1549 c, t dbSNP:776401706
1551 1551 a, c dbSNP:770971876
1557 1557 a, g dbSNP:746928218
1563 1563 c, g dbSNP:140791666
1566 1566 c, t dbSNP:771506862
1567 1567 c, t dbSNP:747656720
1573 1573 a, g dbSNP:375187933
1587 1587 c, g dbSNP:778750039
1593 1593 a, g dbSNP:754655629
1594 1594 a, t dbSNP:749728888
1600 1600 c, t dbSNP:535561852
1601 1601 a, c, g, t dbSNP:781687341
1609 1609 -, tcc dbSNP:766416330
1609 1609 c, t dbSNP:145043679
1610 1610 a, g dbSNP:751604696
1611 1611 c, g dbSNP:201716149
1614 1614 c, g, t dbSNP:139721775
1615 1615 a, g dbSNP:80338864
1618 1618 c, t dbSNP:758040709
1621 1621 c, t dbSNP:765154144
1622 1622 a, g, t dbSNP:542266962
1623 1623 c, g dbSNP:772016342
1624 1624 c, t dbSNP:761458977
1627 1627 a, g dbSNP:140400648
1631 1631 c, g dbSNP:768489010
1635 1635 a, g dbSNP:147850435
1638 1638 c, t dbSNP:557097410
1639 1639 a, g dbSNP:201847193
1640 1640 g, t dbSNP:746201510
1641 1641 c, g, t dbSNP:144562471
1642 1642 c, t dbSNP:371873032
1652 1652 a, g dbSNP:777838196
1654 1654 c, t dbSNP:199506852
1657 1657 c, t dbSNP:201270451
1662 1662 c, t dbSNP:200477386
1663 1663 a, g dbSNP:566784842
1665 1665 c, t dbSNP:368281869
1669 1669 a, g dbSNP:760428437
1672 1672 a, c dbSNP:750211727
1678 1678 c, t dbSNP:148660993
1679 1679 a, c, g dbSNP:201150384
1683 1683 a, g dbSNP:375993195
1685 1685 c, t dbSNP:766709599
1699 1699 c, t dbSNP:775034584
1704 1704 a, g dbSNP:769454033
1705 1705 c, t dbSNP:371307569
1706 1706 -, ggca dbSNP:773237938
1708 1708 a, g dbSNP:537515766
1710 1710 c, t dbSNP:570180636
1721 1721 g, t dbSNP:747532529
1722 1722 c, t dbSNP:753568521
1726 1726 a, g dbSNP:778207094
1729 1729 a, g, t dbSNP:114143715
1731 1731 a, g, t dbSNP:755215281
1731 1731 -, g dbSNP:769745063
1742 1742 c, t dbSNP:750890505
1743 1743 a, g dbSNP:201871263
1751 1751 c, t dbSNP:757059514
1758 1758 c, t dbSNP:182965373
1765 1765 c, g dbSNP:546830267
1784 1784 c, t dbSNP:11555218
1786 1786 c, t dbSNP:528375215
1820 1820 a, g dbSNP:151230950
1821 1821 a, t dbSNP:542228378
1832 1832 c, t dbSNP:143459841
1850 1850 c, t dbSNP:751563187
1858 1858 -, cgctctgttcct dbSNP:373023739
1859 1859 -, gctctgttcctt dbSNP:368755383
1859 1859 a, g dbSNP:563020897
1862 1862 -, ctgttccttgct dbSNP:141483210
1862 1862 c, t dbSNP:200229535
1873 1873 -, ctgttccttgct dbSNP:753444031
1875 1875 c, t dbSNP:762590224
1878 1878 c, t dbSNP:775040338
1879 1879 a, c dbSNP:764851572
1883 1883 a, t dbSNP:759235974
1893 1893 c, t dbSNP:115338563
1894 1894 a, g dbSNP:771310150
1896 1896 a, g dbSNP:578117764
1897 1897 c, t dbSNP:747336124
1901 1901 c, t dbSNP:113188310
1927 1927 c, t dbSNP:185557595
1975 1975 c, t dbSNP:748234524
2005 2005 g, t dbSNP:1802125
2025 2025 c, t dbSNP:759058043
2034 2034 -, ct dbSNP:763034353
2059 2059 c, t dbSNP:78575838
2104 2104 a, c dbSNP:550781403
2117 2117 a, g dbSNP:180885292
2118 2118 -, a dbSNP:34995793
2118 2118 c, t dbSNP:537429160
2122 2122 c, g dbSNP:570096062
2123 2123 a, g dbSNP:529448827
2152 2152 a, g dbSNP:11233662
2181 2181 c, t dbSNP:1790345
2202 2202 a, g dbSNP:370708079
2224 2224 a, g dbSNP:546497674
2253 2253 g, t dbSNP:528351472
2266 2266 a, g dbSNP:79320071
2314 2314 a, g dbSNP:548893003
2341 2341 a, c dbSNP:530422056
2344 2344 c, t dbSNP:1044535
2350 2350 g, t dbSNP:368948615
2366 2366 a, g dbSNP:576060258
2390 2390 -, c dbSNP:34258235
2435 2435 a, c, g, t dbSNP:7690
2443 2443 g, t dbSNP:1802378
2447 2447 a, g dbSNP:564337568
2456 2456 c, t dbSNP:58046295
2457 2457 a, g dbSNP:546098047
2465 2465 c, t dbSNP:559871324
2466 2466 a, g dbSNP:777137931
2480 2480 c, t dbSNP:541282653
2484 2484 a, g dbSNP:747769421
2491 2491 c, t dbSNP:12407
2507 2507 a, g dbSNP:374355489
2528 2528 a, t dbSNP:146774511
2530 2530 a, g dbSNP:190840764
2590 2590 a, g dbSNP:185936568
2640 2640 a, g dbSNP:143699520
2642 2642 a, g dbSNP:576498440
2661 2661 a, t dbSNP:758713975

Target ORF information:

RefSeq Version NM_001360
Organism Homo sapiens (human)
Definition Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001360

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21071
Accession Version NM_001163817.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 7-dehydrocholesterol reductase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA502590.1, AP002387.4 and AI888720.1. Summary: This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000054.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157289.1
CDS251..1678
Misc Feature(1)170..172(+)
Misc Feature(2)290..292(+)
Misc Feature(3)368..430(+)
Misc Feature(4)527..1675(+)
Misc Feature(5)710..772(+)
Misc Feature(6)779..841(+)
Misc Feature(7)1046..1108(+)
Misc Feature(8)1166..1228(+)
Misc Feature(9)1241..1303(+)
Misc Feature(10)1508..1570(+)
Exon (1)1..119
Gene:DHCR7
Gene Synonym:
Exon (2)120..244
Gene:DHCR7
Gene Synonym:
Exon (3)245..348
Gene:DHCR7
Gene Synonym:
Exon (4)349..571
Gene:DHCR7
Gene Synonym:
Exon (5)572..662
Gene:DHCR7
Gene Synonym:
Exon (6)663..876
Gene:DHCR7
Gene Synonym:
Exon (7)877..1081
Gene:DHCR7
Gene Synonym:
Exon (8)1082..1213
Gene:DHCR7
Gene Synonym:
Exon (9)1214..2642
Gene:DHCR7
Gene Synonym:
Translation
Position Chain Variation Link
49 49 c, g, t dbSNP:4944946
67 67 g, t dbSNP:527301706
79 79 g, t dbSNP:141057811
201 201 a, g dbSNP:556280113
216 216 c, t dbSNP:75974711
217 217 g, t dbSNP:145915789
219 219 c, t dbSNP:762318777
228 228 c, t dbSNP:12573951
251 251 a, g dbSNP:104886033
253 253 a, g dbSNP:121909767
262 262 a, g dbSNP:757934834
264 264 c, g, t dbSNP:1127869
265 265 a, g dbSNP:147038941
268 268 a, g dbSNP:754333005
271 271 a, c dbSNP:766817989
273 273 a, g dbSNP:756194690
275 275 a, g, t dbSNP:115595829
278 278 c, g dbSNP:139166382
280 280 c, g dbSNP:775421040
288 288 a, g dbSNP:764925295
295 295 a, g dbSNP:150214075
301 301 c, t dbSNP:776713087
302 302 a, g dbSNP:770947907
304 304 c, t dbSNP:746536172
308 308 a, g dbSNP:772572550
310 310 -, a dbSNP:749174332
310 310 a, t dbSNP:771795285
319 319 c, t dbSNP:199798127
320 320 a, g, t dbSNP:146867923
321 321 a, c dbSNP:530687139
327 327 a, c dbSNP:199815542
329 329 a, g dbSNP:780570846
332 332 c, t dbSNP:756564881
334 334 a, g dbSNP:372855459
339 339 c, g dbSNP:200334114
341 341 a, c, t dbSNP:367585401
342 342 a, g dbSNP:370307688
345 345 c, t dbSNP:780180501
361 361 a, g dbSNP:750345068
368 368 c, t dbSNP:767425988
372 372 c, t dbSNP:761265690
373 373 a, g, t dbSNP:376263149
376 376 c, t dbSNP:150928869
377 377 a, g dbSNP:200984695
381 381 c, t dbSNP:142897396
387 387 g, t dbSNP:746175164
389 389 c, t dbSNP:140721259
397 397 c, t dbSNP:771419549
398 398 a, g dbSNP:747103823
399 399 a, c dbSNP:104886034
400 400 c, t dbSNP:540098203
401 401 c, t dbSNP:104886035
403 403 c, t dbSNP:777966196
406 406 c, g dbSNP:758757355
409 409 c, t dbSNP:752952700
410 410 a, g dbSNP:779222334
412 412 c, t dbSNP:755869989
415 415 c, g dbSNP:374941029
422 422 a, g, t dbSNP:104886032
426 426 g, t dbSNP:104886036
432 432 g, t dbSNP:761788547
435 435 a, t dbSNP:104886037
436 436 c, t dbSNP:763591043
439 439 a, g, t dbSNP:1044482
448 448 c, t dbSNP:775127532
449 449 a, g dbSNP:143999854
453 453 c, t dbSNP:104886038
457 457 c, t dbSNP:1790334
458 458 a, c, g dbSNP:144512551
460 460 c, t dbSNP:747544280
470 470 a, g dbSNP:773481689
471 471 a, c dbSNP:374874199
472 472 c, g, t dbSNP:183441430
473 473 a, t dbSNP:370748173
475 475 c, t dbSNP:755182484
476 476 a, g dbSNP:368473756
480 480 c, t dbSNP:781010025
481 481 c, t dbSNP:4316537
482 482 a, g dbSNP:373352413
491 491 c, g, t dbSNP:775735710
492 492 a, g dbSNP:752153214
498 498 c, t dbSNP:764847274
499 499 a, g dbSNP:759192320
501 501 a, g dbSNP:776923495
502 502 c, t dbSNP:766749461
515 515 a, t dbSNP:375997113
517 517 c, t dbSNP:760887459
518 518 c, t dbSNP:773862228
523 523 c, t dbSNP:772671149
528 528 c, t dbSNP:80338853
529 529 a, g dbSNP:774554996
532 532 a, g dbSNP:541593878
538 538 c, t dbSNP:745595242
539 539 a, g dbSNP:150563256
541 541 c, t dbSNP:780909138
542 542 c, t dbSNP:104886039
546 546 c, t dbSNP:104886041
553 553 c, t dbSNP:777709295
571 571 c, g dbSNP:104886040
574 574 a, g dbSNP:764443524
576 576 c, t dbSNP:121912195
577 577 c, t dbSNP:763112867
583 583 c, t dbSNP:775407178
585 585 c, t dbSNP:770040020
586 586 a, g dbSNP:759750125
592 592 a, c dbSNP:773047702
598 598 c, t dbSNP:373808237
605 605 -, c dbSNP:747827699
606 606 a, t dbSNP:28938174
608 608 a, g dbSNP:771866134
610 610 a, g dbSNP:201227366
619 619 c, g, t dbSNP:373452254
620 620 c, g dbSNP:748879289
625 625 c, t dbSNP:779401555
626 626 a, g dbSNP:143587828
634 634 c, t dbSNP:750090808
635 635 a, c, g dbSNP:138215017
636 636 c, t dbSNP:757703242
645 645 a, g dbSNP:751818759
646 646 a, g dbSNP:764707624
647 647 a, g dbSNP:763333510
649 649 c, t dbSNP:147424205
650 650 -, gtgactcc dbSNP:754560074
650 650 g, t dbSNP:201466849
658 658 g, t dbSNP:772940271
659 659 g, t dbSNP:563056315
665 665 a, g dbSNP:769816544
668 668 a, g dbSNP:373908315
681 681 a, g dbSNP:776246504
688 688 c, g, t dbSNP:949177
690 690 a, g dbSNP:777425801
692 692 c, t dbSNP:758693177
702 702 a, c, g dbSNP:11555217
703 703 a, g dbSNP:104894213
710 710 a, g dbSNP:779417085
711 711 c, g, t dbSNP:143312232
720 720 c, t dbSNP:753960624
721 721 c, g dbSNP:149164866
724 724 g, t dbSNP:766443353
725 725 c, t dbSNP:756416682
733 733 c, t dbSNP:750628746
734 734 a, g dbSNP:767716202
735 735 c, t dbSNP:398123606
738 738 a, g dbSNP:752494910
740 740 c, t dbSNP:774978807
742 742 c, g dbSNP:759538408
750 750 c, g dbSNP:776334336
756 756 c, g, t dbSNP:80338855
757 757 a, g dbSNP:138185442
759 759 c, g dbSNP:150459687
763 763 c, t dbSNP:566499249
764 764 a, g dbSNP:748562900
767 767 a, t dbSNP:779219548
771 771 c, t dbSNP:769218623
772 772 c, t dbSNP:140648594
773 773 a, g dbSNP:368269558
788 788 -, cac dbSNP:779528392
793 793 c, g dbSNP:146839126
795 795 a, g dbSNP:536394774
799 799 c, t dbSNP:115446684
808 808 c, t dbSNP:757519627
819 819 c, t dbSNP:752603560
820 820 c, t dbSNP:74909468
821 821 a, g dbSNP:532635888
824 824 c, t dbSNP:753830704
825 825 c, t dbSNP:766299724
826 826 c, t dbSNP:760123718
829 829 c, g dbSNP:778664009
832 832 c, t dbSNP:150603941
833 833 a, g dbSNP:151170252
836 836 a, g dbSNP:200474791
837 837 c, t dbSNP:142138656
851 851 c, t dbSNP:774755673
853 853 a, c dbSNP:377727130
857 857 c, t dbSNP:749591513
865 865 c, t dbSNP:561379509
866 866 a, g dbSNP:542744675
874 874 c, t dbSNP:374640274
888 888 a, g dbSNP:769583377
895 895 a, c dbSNP:773048017
901 901 a, c dbSNP:749076525
905 905 g, t dbSNP:779896782
907 907 c, t dbSNP:376031781
908 908 a, c, g dbSNP:200659923
910 910 g, t dbSNP:767377692
911 911 a, g dbSNP:756795175
915 915 a, g dbSNP:751026224
916 916 c, t dbSNP:763787299
920 920 a, g dbSNP:373121544
932 932 c, t dbSNP:775773057
933 933 a, g dbSNP:201556114
937 937 a, c, g, t dbSNP:267603172
938 938 a, g dbSNP:747133004
940 940 c, g dbSNP:773393807
942 942 a, g dbSNP:772491341
944 944 c, t dbSNP:748390752
955 955 c, t dbSNP:778945317
959 959 c, t dbSNP:755941916
969 969 a, g dbSNP:148609143
973 973 g, t dbSNP:369654651
974 974 c, t dbSNP:80338856
975 975 a, g dbSNP:80338857
978 978 c, g dbSNP:777248132
979 979 c, t dbSNP:145284180
980 980 a, g dbSNP:121909764
985 985 c, t dbSNP:12800
986 986 a, g dbSNP:759967245
988 988 c, t dbSNP:536834609
989 989 a, g dbSNP:767031102
991 991 c, g, t dbSNP:773485715
994 994 g, t dbSNP:104894212
998 998 c, g dbSNP:772294537
1001 1001 a, g dbSNP:761732160
1006 1006 c, t dbSNP:774666247
1012 1012 c, g, t dbSNP:113950278
1015 1015 c, t dbSNP:200132007
1016 1016 a, g, t dbSNP:772639348
1020 1020 c, t dbSNP:770925697
1021 1021 a, g dbSNP:200232012
1028 1028 c, g dbSNP:777617141
1029 1029 a, g dbSNP:551260416
1031 1031 a, g dbSNP:752096930
1033 1033 a, g dbSNP:778674271
1034 1034 c, g dbSNP:754553051
1039 1039 c, t dbSNP:754320411
1040 1040 a, g dbSNP:766838675
1053 1053 a, g dbSNP:761064004
1054 1054 g, t dbSNP:750973099
1064 1064 c, t dbSNP:767872725
1066 1066 a, g dbSNP:761978602
1070 1070 -, aacgtc dbSNP:757697462
1071 1071 a, c dbSNP:774275482
1072 1072 a, c, t dbSNP:139787408
1073 1073 a, g dbSNP:775628929
1078 1078 a, g dbSNP:770713196
1085 1085 a, g dbSNP:752010614
1089 1089 a, g dbSNP:121909766
1090 1090 c, t dbSNP:148468879
1091 1091 a, g dbSNP:398123607
1102 1102 a, c dbSNP:184297154
1105 1105 c, t dbSNP:781026169
1110 1110 a, g dbSNP:765481717
1111 1111 a, c, t dbSNP:766495775
1112 1112 a, g dbSNP:565893436
1116 1116 c, t dbSNP:121909765
1117 1117 c, t dbSNP:761602799
1120 1120 a, g dbSNP:774187452
1124 1124 c, t dbSNP:768668145
1132 1132 c, t dbSNP:748858240
1133 1133 a, g dbSNP:201574502
1144 1144 c, t dbSNP:769639753
1145 1145 c, t dbSNP:745498103
1156 1156 c, g, t dbSNP:80338858
1157 1157 a, g, t dbSNP:142808899
1177 1177 a, c, t dbSNP:149974099
1178 1178 a, g dbSNP:370955781
1197 1197 a, g dbSNP:754992933
1206 1206 c, g, t dbSNP:766583874
1207 1207 a, g, t dbSNP:200157761
1226 1226 g, t dbSNP:80338859
1232 1232 c, g dbSNP:775805851
1237 1237 c, t dbSNP:145901607
1238 1238 a, c, g dbSNP:139724817
1250 1250 a, g dbSNP:757518371
1251 1251 c, t dbSNP:748020179
1252 1252 a, c dbSNP:778753729
1254 1254 c, g, t dbSNP:77762671
1255 1255 a, g dbSNP:768765843
1258 1258 c, t dbSNP:75225632
1259 1259 a, g dbSNP:749913821
1261 1261 c, t dbSNP:369382960
1262 1262 a, g dbSNP:72954276
1267 1267 c, t dbSNP:774757811
1268 1268 a, g dbSNP:148081697
1270 1270 c, t dbSNP:763165078
1275 1275 c, t dbSNP:199957106
1285 1285 c, g dbSNP:776099806
1290 1290 g, t dbSNP:770174208
1292 1292 -, ctggtgggct dbSNP:767602487
1302 1302 c, t dbSNP:779980243
1304 1304 c, t dbSNP:80338860
1305 1305 a, g, t dbSNP:121909768
1307 1307 -, g dbSNP:759720450
1309 1309 a, g dbSNP:747245607
1310 1310 a, g dbSNP:571923299
1312 1312 c, g dbSNP:145651408
1316 1316 -, c dbSNP:774291653
1321 1321 g, t dbSNP:768526200
1324 1324 a, g dbSNP:748986631
1333 1333 a, c dbSNP:780088227
1334 1334 c, t dbSNP:371302153
1335 1335 a, g, t dbSNP:142213147
1337 1337 c, t dbSNP:547012639
1338 1338 a, g dbSNP:200539324
1341 1341 c, t dbSNP:567600444
1342 1342 a, g dbSNP:35946774
1349 1349 c, t dbSNP:531038145
1350 1350 a, g dbSNP:765908713
1364 1364 g, t dbSNP:760104729
1376 1376 a, g dbSNP:777218262
1377 1377 a, g dbSNP:771137757
1384 1384 c, g, t dbSNP:773505265
1385 1385 a, g dbSNP:772162793
1388 1388 c, t dbSNP:373306653
1389 1389 a, g, t dbSNP:779709646
1390 1390 a, c dbSNP:769783115
1398 1398 c, t dbSNP:745820609
1401 1401 c, g dbSNP:781043327
1402 1402 c, t dbSNP:145084285
1403 1403 a, g dbSNP:750989470
1404 1404 c, g dbSNP:12577137
1405 1405 c, t dbSNP:777517985
1406 1406 a, g dbSNP:369837196
1408 1408 a, c, t dbSNP:760241
1409 1409 a, g dbSNP:765707139
1416 1416 a, g dbSNP:755325060
1418 1418 c, t dbSNP:544442568
1419 1419 a, g dbSNP:766943022
1437 1437 a, t dbSNP:760857590
1440 1440 c, t dbSNP:773134475
1441 1441 a, g dbSNP:767847651
1448 1448 g, t dbSNP:78523425
1453 1453 c, t dbSNP:368867929
1454 1454 a, c, g dbSNP:200099137
1460 1460 c, t dbSNP:61757582
1465 1465 c, g dbSNP:776577137
1469 1469 a, t dbSNP:770819693
1470 1470 a, g dbSNP:746591926
1474 1474 c, t dbSNP:777148033
1475 1475 a, g dbSNP:757861528
1478 1478 a, g dbSNP:80338862
1481 1481 a, g dbSNP:372055524
1490 1490 c, g dbSNP:755426995
1493 1493 a, g dbSNP:754224400
1501 1501 c, t dbSNP:767043016
1510 1510 a, g dbSNP:756564409
1514 1514 c, t dbSNP:750582708
1519 1519 c, t dbSNP:145374203
1520 1520 a, g, t dbSNP:368150818
1522 1522 c, t dbSNP:909217
1523 1523 a, g dbSNP:760242
1526 1526 c, t dbSNP:776401706
1528 1528 a, c dbSNP:770971876
1534 1534 a, g dbSNP:746928218
1540 1540 c, g dbSNP:140791666
1543 1543 c, t dbSNP:771506862
1544 1544 c, t dbSNP:747656720
1550 1550 a, g dbSNP:375187933
1564 1564 c, g dbSNP:778750039
1570 1570 a, g dbSNP:754655629
1571 1571 a, t dbSNP:749728888
1577 1577 c, t dbSNP:535561852
1578 1578 a, c, g, t dbSNP:781687341
1586 1586 -, tcc dbSNP:766416330
1586 1586 c, t dbSNP:145043679
1587 1587 a, g dbSNP:751604696
1588 1588 c, g dbSNP:201716149
1591 1591 c, g, t dbSNP:139721775
1592 1592 a, g dbSNP:80338864
1595 1595 c, t dbSNP:758040709
1598 1598 c, t dbSNP:765154144
1599 1599 a, g, t dbSNP:542266962
1600 1600 c, g dbSNP:772016342
1601 1601 c, t dbSNP:761458977
1604 1604 a, g dbSNP:140400648
1608 1608 c, g dbSNP:768489010
1612 1612 a, g dbSNP:147850435
1615 1615 c, t dbSNP:557097410
1616 1616 a, g dbSNP:201847193
1617 1617 g, t dbSNP:746201510
1618 1618 c, g, t dbSNP:144562471
1619 1619 c, t dbSNP:371873032
1629 1629 a, g dbSNP:777838196
1631 1631 c, t dbSNP:199506852
1634 1634 c, t dbSNP:201270451
1639 1639 c, t dbSNP:200477386
1640 1640 a, g dbSNP:566784842
1642 1642 c, t dbSNP:368281869
1646 1646 a, g dbSNP:760428437
1649 1649 a, c dbSNP:750211727
1655 1655 c, t dbSNP:148660993
1656 1656 a, c, g dbSNP:201150384
1660 1660 a, g dbSNP:375993195
1662 1662 c, t dbSNP:766709599
1676 1676 c, t dbSNP:775034584
1681 1681 a, g dbSNP:769454033
1682 1682 c, t dbSNP:371307569
1683 1683 -, ggca dbSNP:773237938
1685 1685 a, g dbSNP:537515766
1687 1687 c, t dbSNP:570180636
1698 1698 g, t dbSNP:747532529
1699 1699 c, t dbSNP:753568521
1703 1703 a, g dbSNP:778207094
1706 1706 a, g, t dbSNP:114143715
1708 1708 a, g, t dbSNP:755215281
1708 1708 -, g dbSNP:769745063
1719 1719 c, t dbSNP:750890505
1720 1720 a, g dbSNP:201871263
1728 1728 c, t dbSNP:757059514
1735 1735 c, t dbSNP:182965373
1742 1742 c, g dbSNP:546830267
1761 1761 c, t dbSNP:11555218
1763 1763 c, t dbSNP:528375215
1797 1797 a, g dbSNP:151230950
1798 1798 a, t dbSNP:542228378
1809 1809 c, t dbSNP:143459841
1827 1827 c, t dbSNP:751563187
1835 1835 -, cgctctgttcct dbSNP:373023739
1836 1836 -, gctctgttcctt dbSNP:368755383
1836 1836 a, g dbSNP:563020897
1839 1839 -, ctgttccttgct dbSNP:141483210
1839 1839 c, t dbSNP:200229535
1850 1850 -, ctgttccttgct dbSNP:753444031
1852 1852 c, t dbSNP:762590224
1855 1855 c, t dbSNP:775040338
1856 1856 a, c dbSNP:764851572
1860 1860 a, t dbSNP:759235974
1870 1870 c, t dbSNP:115338563
1871 1871 a, g dbSNP:771310150
1873 1873 a, g dbSNP:578117764
1874 1874 c, t dbSNP:747336124
1878 1878 c, t dbSNP:113188310
1904 1904 c, t dbSNP:185557595
1952 1952 c, t dbSNP:748234524
1982 1982 g, t dbSNP:1802125
2002 2002 c, t dbSNP:759058043
2011 2011 -, ct dbSNP:763034353
2036 2036 c, t dbSNP:78575838
2081 2081 a, c dbSNP:550781403
2094 2094 a, g dbSNP:180885292
2095 2095 -, a dbSNP:34995793
2095 2095 c, t dbSNP:537429160
2099 2099 c, g dbSNP:570096062
2100 2100 a, g dbSNP:529448827
2129 2129 a, g dbSNP:11233662
2158 2158 c, t dbSNP:1790345
2179 2179 a, g dbSNP:370708079
2201 2201 a, g dbSNP:546497674
2230 2230 g, t dbSNP:528351472
2243 2243 a, g dbSNP:79320071
2291 2291 a, g dbSNP:548893003
2318 2318 a, c dbSNP:530422056
2321 2321 c, t dbSNP:1044535
2327 2327 g, t dbSNP:368948615
2343 2343 a, g dbSNP:576060258
2367 2367 -, c dbSNP:34258235
2412 2412 a, c, g, t dbSNP:7690
2420 2420 g, t dbSNP:1802378
2424 2424 a, g dbSNP:564337568
2433 2433 c, t dbSNP:58046295
2434 2434 a, g dbSNP:546098047
2442 2442 c, t dbSNP:559871324
2443 2443 a, g dbSNP:777137931
2457 2457 c, t dbSNP:541282653
2461 2461 a, g dbSNP:747769421
2468 2468 c, t dbSNP:12407
2484 2484 a, g dbSNP:374355489
2505 2505 a, t dbSNP:146774511
2507 2507 a, g dbSNP:190840764
2567 2567 a, g dbSNP:185936568
2617 2617 a, g dbSNP:143699520
2619 2619 a, g dbSNP:576498440
2638 2638 a, t dbSNP:758713975

Target ORF information:

RefSeq Version NM_001163817
Organism Homo sapiens (human)
Definition Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163817

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.