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DLAT dihydrolipoamide S-acetyltransferase [Homo sapiens (human)]

Gene Symbol DLAT
Entrez Gene ID 1737
Full Name dihydrolipoamide S-acetyltransferase
Synonyms DLTA, PDC-E2, PDCE2
General protein information
Preferred Names
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Names
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
PBC
M2 antigen complex 70 kDa subunit
pyruvate dehydrogenase complex component E2
E2 component of pyruvate dehydrogenase complex
70 kDa mitochondrial autoantigen of primary biliary cirrhosis
dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial
NP_001922.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q23.1

Summary This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

608770

Disorder Html: Pyruvate dehydrogenase E2 deficiency, 245348 (3)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu57240 XM_011542647 PREDICTED: Homo sapiens dihydrolipoamide S-acetyltransferase (DLAT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu26089 NM_001931 Homo sapiens dihydrolipoamide S-acetyltransferase (DLAT), mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu57240D
Sequence Information ORF Nucleotide Sequence (Length: 1836bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTGGCGCG TCTGTGCGCG ACGGGCTCAG AATGTAGCCC CATGGGCGGG ACTCGAGGCT 
CGGTGGACGG CCTTGCAGGA GGTACCCGGA ACTCCACGAG TGACCTCGCG ATCTGGCCCG
GCTCCCGCTC GTCGCAACAG CGTGACTACA GGGTATGGCG GGGTCCGGGC ACTGTGCGGC
TGGACCCCCA GTTCTGGGGC CACGCCGCGG AACCGCTTAC TGCTGCAGCT TTTGGGGTCG
CCCGGCCGCC GCTATTACAG TCTTCCCCCG CATCAGAAGG TTCCATTGCC TTCTCTTTCC
CCCACAATGC AGGCAGGCAC CATAGCCCGT TGGGAAAAAA AAGAGGGGGA CAAAATCAAT
GAAGGTGACC TAATTGCAGA GGTTGAAACT GATAAAGCCA CTGTTGGATT TGAGAGCCTG
GAGGAGTGTT ATATGGCAAA GATACTTGTT GCTGAAGGTA CCAGGGATGT TCCCATCGGA
GCGATCATCT GTATCACAGT TGGCAAGCCT GAGGATATTG AGGCCTTTAA AAATTATACA
CTGGATTCCT CAGCAGCACC TACCCCACAA GCGGCCCCAG CACCAACCCC TGCTGCCACT
GCTTCGCCAC CTACACCTTC TGCTCAGGCT CCTGGTAGCT CATATCCCCC TCACATGCAG
GTACTTCTTC CTGCCCTCTC TCCCACCATG ACCATGGGCA CAGTTCAGAG ATGGGAAAAA
AAAGTGGGTG AGAAGCTAAG TGAAGGAGAC TTACTGGCAG AGATAGAAAC TGACAAAGCC
ACTATAGGTT TTGAAGTACA GGAAGAAGGT TATCTGGCAA AAATCCTGGT CCCTGAAGGC
ACAAGAGATG TCCCTCTAGG AACCCCACTC TGTATCATTG TAGAAAAAGA GGCAGATATA
TCAGCATTTG CTGACTATAG GCCAACCGAA GTAACAGATT TAAAACCACA AGTGCCACCA
CCTACCCCAC CCCCGGTGGC CGCTGTTCCT CCAACTCCCC AGCCTTTAGC TCCTACACCT
TCAGCACCCT GCCCAGCTAC TCCTGCTGGA CCAAAGGGAA GGGTGTTTGT TAGCCCTCTT
GCAAAGAAGT TGGCAGTAGA GAAAGGGATT GATCTTACAC AAGTAAAAGG GACAGGACCA
GATGGTAGAA TCACCAAGAA GGATATCGAC TCTTTTGTGC CTAGTAAAGT TGCTCCTGCT
CCGGCAGCTG TTGTGCCTCC CACAGGTCCT GGAATGGCAC CAGTTCCTAC AGGTGTCTTC
ACAGATATCC CAATCAGCAA CATTCGTCGG ATATTAGAAG GGAGAAGCAA AATTTCTGTC
AATGACTTCA TCATAAAAGC TTCAGCTTTG GCATGTTTAA AAGTTCCCGA AGCAAATTCT
TCTTGGATGG ACACAGTTAT AAGACAAAAT CATGTTGTTG ATGTCAGTGT TGCGGTCAGT
ACTCCTGCAG GACTCATCAC ACCTATTGTG TTTAATGCAC ATATAAAAGG AGTGGAAACC
ATTGCTAATG ATGTTGTTTC TTTAGCAACC AAAGCAAGAG AGGGTAAACT ACAGCCACAT
GAATTCCAGG GTGGCACTTT TACGATCTCC AATTTAGGAA TGTTTGGAAT TAAGAATTTC
TCTGCTATTA TTAACCCACC TCAAGCATGT ATTTTGGCAA TTGGTGCTTC AGAGGATAAA
CTGGTCCCTG CAGATAATGA AAAAGGGTTT GATGTGGCTA GCATGATGTC TGTTACACTC
AGTTGTGATC ACCGGGTGGT GGATGGAGCA GTTGGAGCCC AGTGGCTTGC TGAGTTTAGA
AAGTACCTTG AAAAACCTAT CACTATGTTG TTGTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011540949.1
CDS104..1939
Misc Feature(1)377..598(+)
Misc Feature(2)467..517(+)
Misc Feature(3)497..499(+)
Misc Feature(4)758..982(+)
Misc Feature(5)764..1936(+)
Misc Feature(6)848..898(+)
Misc Feature(7)878..880(+)
Misc Feature(8)1160..1276(+)
Misc Feature(9)1358..1936(+)
Translation
Position Chain Variation Link
74 74 a, g dbSNP:748446519
81 81 a, g dbSNP:772384454
149 149 a, g dbSNP:150145390
158 158 c, g dbSNP:61757217
231 231 c, t dbSNP:2303436
455 455 a, c dbSNP:2553933
627 627 a, c, g dbSNP:201046000
673 673 a, g dbSNP:143107853
729 729 a, g dbSNP:11553595
796 796 c, t dbSNP:34680691
856 856 a, g dbSNP:201257054
860 860 g, t dbSNP:746422062
1031 1031 a, g dbSNP:116125936
1056 1056 c, g, t dbSNP:627441
1094 1094 c, g dbSNP:78320677
1306 1306 a, g dbSNP:200907695
1530 1530 a, g dbSNP:113521778
1723 1723 a, c dbSNP:119103240
2106 2106 c, t dbSNP:142746877
2358 2358 a, g dbSNP:9371

Target ORF information:

RefSeq Version XM_011542647
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens dihydrolipoamide S-acetyltransferase (DLAT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011542647

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTGGCGCG TCTGTGCGCG ACGGGCTCAG AATGTAGCCC CATGGGCGGG ACTCGAGGCT 
CGGTGGACGG CCTTGCAGGA GGTACCCGGA ACTCCACGAG TGACCTCGCG ATCTGGCCCG
GCTCCCGCTC GTCGCAACAG CGTGACTACA GGGTATGGCG GGGTCCGGGC ACTGTGCGGC
TGGACCCCCA GTTCTGGGGC CACGCCGCGG AACCGCTTAC TGCTGCAGCT TTTGGGGTCG
CCCGGCCGCC GCTATTACAG TCTTCCCCCG CATCAGAAGG TTCCATTGCC TTCTCTTTCC
CCCACAATGC AGGCAGGCAC CATAGCCCGT TGGGAAAAAA AAGAGGGGGA CAAAATCAAT
GAAGGTGACC TAATTGCAGA GGTTGAAACT GATAAAGCCA CTGTTGGATT TGAGAGCCTG
GAGGAGTGTT ATATGGCAAA GATACTTGTT GCTGAAGGTA CCAGGGATGT TCCCATCGGA
GCGATCATCT GTATCACAGT TGGCAAGCCT GAGGATATTG AGGCCTTTAA AAATTATACA
CTGGATTCCT CAGCAGCACC TACCCCACAA GCGGCCCCAG CACCAACCCC TGCTGCCACT
GCTTCGCCAC CTACACCTTC TGCTCAGGCT CCTGGTAGCT CATATCCCCC TCACATGCAG
GTACTTCTTC CTGCCCTCTC TCCCACCATG ACCATGGGCA CAGTTCAGAG ATGGGAAAAA
AAAGTGGGTG AGAAGCTAAG TGAAGGAGAC TTACTGGCAG AGATAGAAAC TGACAAAGCC
ACTATAGGTT TTGAAGTACA GGAAGAAGGT TATCTGGCAA AAATCCTGGT CCCTGAAGGC
ACAAGAGATG TCCCTCTAGG AACCCCACTC TGTATCATTG TAGAAAAAGA GGCAGATATA
TCAGCATTTG CTGACTATAG GCCAACCGAA GTAACAGATT TAAAACCACA AGTGCCACCA
CCTACCCCAC CCCCGGTGGC CGCTGTTCCT CCAACTCCCC AGCCTTTAGC TCCTACACCT
TCAGCACCCT GCCCAGCTAC TCCTGCTGGA CCAAAGGGAA GGGTGTTTGT TAGCCCTCTT
GCAAAGAAGT TGGCAGTAGA GAAAGGGATT GATCTTACAC AAGTAAAAGG GACAGGACCA
GATGGTAGAA TCACCAAGAA GGATATCGAC TCTTTTGTGC CTAGTAAAGT TGCTCCTGCT
CCGGCAGCTG TTGTGCCTCC CACAGGTCCT GGAATGGCAC CAGTTCCTAC AGGTGTCTTC
ACAGATATCC CAATCAGCAA CATTCGTCGG ATATTAGAAG GGAGAAGCAA AATTTCTGTC
AATGACTTCA TCATAAAAGC TTCAGCTTTG GCATGTTTAA AAGTTCCCGA AGCAAATTCT
TCTTGGATGG ACACAGTTAT AAGACAAAAT CATGTTGTTG ATGTCAGTGT TGCGGTCAGT
ACTCCTGCAG GACTCATCAC ACCTATTGTG TTTAATGCAC ATATAAAAGG AGTGGAAACC
ATTGCTAATG ATGTTGTTTC TTTAGCAACC AAAGCAAGAG AGGGTAAACT ACAGCCACAT
GAATTCCAGG GTGGCACTTT TACGATCTCC AATTTAGGAA TGTTTGGAAT TAAGAATTTC
TCTGCTATTA TTAACCCACC TCAAGCATGT ATTTTGGCAA TTGGTGCTTC AGAGGATAAA
CTGGTCCCTG CAGATAATGA AAAAGGGTTT GATGTGGCTA GCATGATGTC TGTTACACTC
AGTTGTGATC ACCGGGTGGT GGATGGAGCA GTTGGAGCCC AGTGGCTTGC TGAGTTTAGA
AAGTACCTTG AAAAACCTAT CACTATGTTG TTGTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu26089D
Sequence Information ORF Nucleotide Sequence (Length: 1944bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057299.1, BC039084.1 and AW188637.1. This sequence is a reference standard in the RefSeqGene project. On Oct 3, 2009 this sequence version replaced gi:189458882. Summary: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK057299.1, BC039084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTGGCGCG TCTGTGCGCG ACGGGCTCAG AATGTAGCCC CATGGGCGGG ACTCGAGGCT 
CGGTGGACGG CCTTGCAGGA GGTACCCGGA ACTCCACGAG TGACCTCGCG ATCTGGCCCG
GCTCCCGCTC GTCGCAACAG CGTGACTACA GGGTATGGCG GGGTCCGGGC ACTGTGCGGC
TGGACCCCCA GTTCTGGGGC CACGCCGCGG AACCGCTTAC TGCTGCAGCT TTTGGGGTCG
CCCGGCCGCC GCTATTACAG TCTTCCCCCG CATCAGAAGG TTCCATTGCC TTCTCTTTCC
CCCACAATGC AGGCAGGCAC CATAGCCCGT TGGGAAAAAA AAGAGGGGGA CAAAATCAAT
GAAGGTGACC TAATTGCAGA GGTTGAAACT GATAAAGCCA CTGTTGGATT TGAGAGCCTG
GAGGAGTGTT ATATGGCAAA GATACTTGTT GCTGAAGGTA CCAGGGATGT TCCCATCGGA
GCGATCATCT GTATCACAGT TGGCAAGCCT GAGGATATTG AGGCCTTTAA AAATTATACA
CTGGATTCCT CAGCAGCACC TACCCCACAA GCGGCCCCAG CACCAACCCC TGCTGCCACT
GCTTCGCCAC CTACACCTTC TGCTCAGGCT CCTGGTAGCT CATATCCCCC TCACATGCAG
GTACTTCTTC CTGCCCTCTC TCCCACCATG ACCATGGGCA CAGTTCAGAG ATGGGAAAAA
AAAGTGGGTG AGAAGCTAAG TGAAGGAGAC TTACTGGCAG AGATAGAAAC TGACAAAGCC
ACTATAGGTT TTGAAGTACA GGAAGAAGGT TATCTGGCAA AAATCCTGGT CCCTGAAGGC
ACAAGAGATG TCCCTCTAGG AACCCCACTC TGTATCATTG TAGAAAAAGA GGCAGATATA
TCAGCATTTG CTGACTATAG GCCAACCGAA GTAACAGATT TAAAACCACA AGTGCCACCA
CCTACCCCAC CCCCGGTGGC CGCTGTTCCT CCAACTCCCC AGCCTTTAGC TCCTACACCT
TCAGCACCCT GCCCAGCTAC TCCTGCTGGA CCAAAGGGAA GGGTGTTTGT TAGCCCTCTT
GCAAAGAAGT TGGCAGTAGA GAAAGGGATT GATCTTACAC AAGTAAAAGG GACAGGACCA
GATGGTAGAA TCACCAAGAA GGATATCGAC TCTTTTGTGC CTAGTAAAGT TGCTCCTGCT
CCGGCAGCTG TTGTGCCTCC CACAGGTCCT GGAATGGCAC CAGTTCCTAC AGGTGTCTTC
ACAGATATCC CAATCAGCAA CATTCGTCGG GTTATTGCAC AGCGATTAAT GCAATCAAAG
CAAACCATAC CTCATTATTA CCTTTCTATC GATGTAAATA TGGGAGAAGT TTTGTTGGTA
CGGAAAGAAC TTAATAAGAT ATTAGAAGGG AGAAGCAAAA TTTCTGTCAA TGACTTCATC
ATAAAAGCTT CAGCTTTGGC ATGTTTAAAA GTTCCCGAAG CAAATTCTTC TTGGATGGAC
ACAGTTATAA GACAAAATCA TGTTGTTGAT GTCAGTGTTG CGGTCAGTAC TCCTGCAGGA
CTCATCACAC CTATTGTGTT TAATGCACAT ATAAAAGGAG TGGAAACCAT TGCTAATGAT
GTTGTTTCTT TAGCAACCAA AGCAAGAGAG GGTAAACTAC AGCCACATGA ATTCCAGGGT
GGCACTTTTA CGATCTCCAA TTTAGGAATG TTTGGAATTA AGAATTTCTC TGCTATTATT
AACCCACCTC AAGCATGTAT TTTGGCAATT GGTGCTTCAG AGGATAAACT GGTCCCTGCA
GATAATGAAA AAGGGTTTGA TGTGGCTAGC ATGATGTCTG TTACACTCAG TTGTGATCAC
CGGGTGGTGG ATGGAGCAGT TGGAGCCCAG TGGCTTGCTG AGTTTAGAAA GTACCTTGAA
AAACCTATCA CTATGTTGTT GTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001922.2
CDS660..2603
Misc Feature(1)582..584(+)
Misc Feature(2)933..1154(+)
Misc Feature(3)1023..1073(+)
Misc Feature(4)1053..1055(+)
Misc Feature(5)1053..1055(+)
Misc Feature(6)1314..1538(+)
Misc Feature(7)1320..2600(+)
Misc Feature(8)1404..1454(+)
Misc Feature(9)1434..1436(+)
Misc Feature(10)1434..1436(+)
Misc Feature(11)1716..1832(+)
Misc Feature(12)1731..1826(+)
Misc Feature(13)1908..2600(+)
Misc Feature(14)1965..2600(+)
Misc Feature(15)2055..2057(+)
Exon (1)1..938
Gene:DLAT
Gene Synonym:
Exon (2)939..1040
Gene:DLAT
Gene Synonym:
Exon (3)1041..1165
Gene:DLAT
Gene Synonym:
Exon (4)1166..1319
Gene:DLAT
Gene Synonym:
Exon (5)1320..1446
Gene:DLAT
Gene Synonym:
Exon (6)1447..1634
Gene:DLAT
Gene Synonym:
Exon (7)1635..1788
Gene:DLAT
Gene Synonym:
Exon (8)1789..1856
Gene:DLAT
Gene Synonym:
Exon (9)1857..1949
Gene:DLAT
Gene Synonym:
Exon (10)1950..2057
Gene:DLAT
Gene Synonym:
Exon (11)2058..2173
Gene:DLAT
Gene Synonym:
Exon (12)2174..2336
Gene:DLAT
Gene Synonym:
Exon (13)2337..2473
Gene:DLAT
Gene Synonym:
Exon (14)2474..4346
Gene:DLAT
Gene Synonym:
Translation
Position Chain Variation Link
62 62 c, t dbSNP:78298568
438 438 c, t dbSNP:745412028
448 448 c, g dbSNP:769386207
476 476 a, g dbSNP:779695757
630 630 a, g dbSNP:748446519
637 637 a, g dbSNP:772384454
705 705 a, g dbSNP:150145390
714 714 c, g dbSNP:61757217
787 787 c, t dbSNP:2303436
1011 1011 a, c dbSNP:2553933
1183 1183 a, c, g dbSNP:201046000
1229 1229 a, g dbSNP:143107853
1285 1285 a, g dbSNP:11553595
1352 1352 c, t dbSNP:34680691
1412 1412 a, g dbSNP:201257054
1416 1416 g, t dbSNP:746422062
1587 1587 a, g dbSNP:116125936
1612 1612 c, g, t dbSNP:627441
1650 1650 c, g dbSNP:78320677
1862 1862 a, g dbSNP:200907695
1998 1998 c, t dbSNP:193921135
2010 2010 a, g dbSNP:10891314
2194 2194 a, g dbSNP:113521778
2387 2387 a, c dbSNP:119103240
2770 2770 c, t dbSNP:142746877
3022 3022 a, g dbSNP:9371
3476 3476 c, t dbSNP:73568056
3712 3712 c, t dbSNP:75608010
3839 3839 a, g dbSNP:200256272
3851 3851 c, g dbSNP:9822
3921 3921 a, g dbSNP:757760280
3955 3955 a, t dbSNP:781760543

Target ORF information:

RefSeq Version NM_001931
Organism Homo sapiens (human)
Definition Homo sapiens dihydrolipoamide S-acetyltransferase (DLAT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001931

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTGGCGCG TCTGTGCGCG ACGGGCTCAG AATGTAGCCC CATGGGCGGG ACTCGAGGCT 
CGGTGGACGG CCTTGCAGGA GGTACCCGGA ACTCCACGAG TGACCTCGCG ATCTGGCCCG
GCTCCCGCTC GTCGCAACAG CGTGACTACA GGGTATGGCG GGGTCCGGGC ACTGTGCGGC
TGGACCCCCA GTTCTGGGGC CACGCCGCGG AACCGCTTAC TGCTGCAGCT TTTGGGGTCG
CCCGGCCGCC GCTATTACAG TCTTCCCCCG CATCAGAAGG TTCCATTGCC TTCTCTTTCC
CCCACAATGC AGGCAGGCAC CATAGCCCGT TGGGAAAAAA AAGAGGGGGA CAAAATCAAT
GAAGGTGACC TAATTGCAGA GGTTGAAACT GATAAAGCCA CTGTTGGATT TGAGAGCCTG
GAGGAGTGTT ATATGGCAAA GATACTTGTT GCTGAAGGTA CCAGGGATGT TCCCATCGGA
GCGATCATCT GTATCACAGT TGGCAAGCCT GAGGATATTG AGGCCTTTAA AAATTATACA
CTGGATTCCT CAGCAGCACC TACCCCACAA GCGGCCCCAG CACCAACCCC TGCTGCCACT
GCTTCGCCAC CTACACCTTC TGCTCAGGCT CCTGGTAGCT CATATCCCCC TCACATGCAG
GTACTTCTTC CTGCCCTCTC TCCCACCATG ACCATGGGCA CAGTTCAGAG ATGGGAAAAA
AAAGTGGGTG AGAAGCTAAG TGAAGGAGAC TTACTGGCAG AGATAGAAAC TGACAAAGCC
ACTATAGGTT TTGAAGTACA GGAAGAAGGT TATCTGGCAA AAATCCTGGT CCCTGAAGGC
ACAAGAGATG TCCCTCTAGG AACCCCACTC TGTATCATTG TAGAAAAAGA GGCAGATATA
TCAGCATTTG CTGACTATAG GCCAACCGAA GTAACAGATT TAAAACCACA AGTGCCACCA
CCTACCCCAC CCCCGGTGGC CGCTGTTCCT CCAACTCCCC AGCCTTTAGC TCCTACACCT
TCAGCACCCT GCCCAGCTAC TCCTGCTGGA CCAAAGGGAA GGGTGTTTGT TAGCCCTCTT
GCAAAGAAGT TGGCAGTAGA GAAAGGGATT GATCTTACAC AAGTAAAAGG GACAGGACCA
GATGGTAGAA TCACCAAGAA GGATATCGAC TCTTTTGTGC CTAGTAAAGT TGCTCCTGCT
CCGGCAGCTG TTGTGCCTCC CACAGGTCCT GGAATGGCAC CAGTTCCTAC AGGTGTCTTC
ACAGATATCC CAATCAGCAA CATTCGTCGG GTTATTGCAC AGCGATTAAT GCAATCAAAG
CAAACCATAC CTCATTATTA CCTTTCTATC GATGTAAATA TGGGAGAAGT TTTGTTGGTA
CGGAAAGAAC TTAATAAGAT ATTAGAAGGG AGAAGCAAAA TTTCTGTCAA TGACTTCATC
ATAAAAGCTT CAGCTTTGGC ATGTTTAAAA GTTCCCGAAG CAAATTCTTC TTGGATGGAC
ACAGTTATAA GACAAAATCA TGTTGTTGAT GTCAGTGTTG CGGTCAGTAC TCCTGCAGGA
CTCATCACAC CTATTGTGTT TAATGCACAT ATAAAAGGAG TGGAAACCAT TGCTAATGAT
GTTGTTTCTT TAGCAACCAA AGCAAGAGAG GGTAAACTAC AGCCACATGA ATTCCAGGGT
GGCACTTTTA CGATCTCCAA TTTAGGAATG TTTGGAATTA AGAATTTCTC TGCTATTATT
AACCCACCTC AAGCATGTAT TTTGGCAATT GGTGCTTCAG AGGATAAACT GGTCCCTGCA
GATAATGAAA AAGGGTTTGA TGTGGCTAGC ATGATGTCTG TTACACTCAG TTGTGATCAC
CGGGTGGTGG ATGGAGCAGT TGGAGCCCAG TGGCTTGCTG AGTTTAGAAA GTACCTTGAA
AAACCTATCA CTATGTTGTT GTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.