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TYMP cDNA ORF clone, Homo sapiens (human)

Gene Symbol TYMP
Entrez Gene ID 1890
Full Name thymidine phosphorylase
Synonyms ECGF, ECGF1, MEDPS1, MNGIE, MTDPS1, PDECGF, TP, hPD-ECGF
General protein information
Preferred Names
thymidine phosphorylase
Names
thymidine phosphorylase
tdRPase
gliostatin
NP_001107227.1
NP_001107228.1
NP_001244917.1
NP_001244918.1
NP_001944.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q13.33

Summary This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]. lac of sum
Disorder MIM:

131222

Disorder Html: Mitochondrial DNA depletion syndrome 1 (MNGIE type), 603041 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001953 NP_001944 thymidine phosphorylase isoform 1 proprotein
NM_001113756 NP_001107228 thymidine phosphorylase isoform 1 proprotein
NM_001257988 NP_001244917 thymidine phosphorylase isoform 1 proprotein
NM_001257989 NP_001244918 thymidine phosphorylase isoform 2 proprotein
NM_001113755 NP_001107227 thymidine phosphorylase isoform 1 proprotein



Homo sapiens (human) TYMP NP_001107228.1
Pan troglodytes (chimpanzee) TYMP XP_515224.4
Macaca mulatta (Rhesus monkey) TYMP XP_001112945.1
Mus musculus (house mouse) Tymp NP_612175.1
Rattus norvegicus (Norway rat) Tymp NP_001012122.1
Xenopus (Silurana) tropicalis (western clawed frog) tymp NP_001120427.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TYMP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TYMP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22602
NM_001953 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22602
NM_001113756 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22602
NM_001257988 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu23077 NM_001257989 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu22602
NM_001113755 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113755 , NM_001113756 , NM_001257988 , NM_001953
Accession Version NM_001953.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thymidine phosphorylase isoform 1 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC018160.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced gi:166158923. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC018160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001944.1
CDS193..1641
Misc Feature(1)97..99(+)
Misc Feature(2)295..489(+)
Misc Feature(3)301..1530(+)
Misc Feature(4)517..1212(+)
Misc Feature(5)985..1029(+)
Misc Feature(6)1177..1218(+)
Misc Feature(7)1357..1578(+)
Misc Feature(8)1369..1395(+)
Misc Feature(9)1549..1575(+)
Exon (1)1..182
Gene:TYMP
Gene Synonym:
Exon (2)183..406
Gene:TYMP
Gene Synonym:
Exon (3)407..609
Gene:TYMP
Gene Synonym:
Exon (4)610..708
Gene:TYMP
Gene Synonym:
Exon (5)709..838
Gene:TYMP
Gene Synonym:
Exon (6)839..957
Gene:TYMP
Gene Synonym:
Exon (7)958..1120
Gene:TYMP
Gene Synonym:
Exon (8)1121..1351
Gene:TYMP
Gene Synonym:
Exon (9)1352..1492
Gene:TYMP
Gene Synonym:
Exon (10)1493..1659
Gene:TYMP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001953
Organism Homo sapiens (human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113755 , NM_001113756 , NM_001257988 , NM_001953
Accession Version NM_001113756.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thymidine phosphorylase isoform 1 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, M63193.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced gi:166158924. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: M63193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107228.1
CDS377..1825
Misc Feature(1)176..178(+)
Misc Feature(2)479..673(+)
Misc Feature(3)485..1714(+)
Misc Feature(4)701..1396(+)
Misc Feature(5)1169..1213(+)
Misc Feature(6)1361..1402(+)
Misc Feature(7)1541..1762(+)
Misc Feature(8)1553..1579(+)
Misc Feature(9)1733..1759(+)
Exon (1)1..590
Gene:TYMP
Gene Synonym:
Exon (2)591..793
Gene:TYMP
Gene Synonym:
Exon (3)794..892
Gene:TYMP
Gene Synonym:
Exon (4)893..1022
Gene:TYMP
Gene Synonym:
Exon (5)1023..1141
Gene:TYMP
Gene Synonym:
Exon (6)1142..1304
Gene:TYMP
Gene Synonym:
Exon (7)1305..1535
Gene:TYMP
Gene Synonym:
Exon (8)1536..1676
Gene:TYMP
Gene Synonym:
Exon (9)1677..1843
Gene:TYMP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001113756
Organism Homo sapiens (human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113756

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113755 , NM_001113756 , NM_001257988 , NM_001953
Accession Version NM_001257988.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thymidine phosphorylase isoform 1 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC052211.2 and AK225269.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC052211.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244917.1
CDS228..1676
Misc Feature(1)330..524(+)
Misc Feature(2)336..1565(+)
Misc Feature(3)552..1247(+)
Misc Feature(4)1020..1064(+)
Misc Feature(5)1212..1253(+)
Misc Feature(6)1392..1613(+)
Misc Feature(7)1404..1430(+)
Misc Feature(8)1584..1610(+)
Exon (1)1..182
Gene:TYMP
Gene Synonym:
Exon (2)183..441
Gene:TYMP
Gene Synonym:
Exon (3)442..644
Gene:TYMP
Gene Synonym:
Exon (4)645..743
Gene:TYMP
Gene Synonym:
Exon (5)744..873
Gene:TYMP
Gene Synonym:
Exon (6)874..992
Gene:TYMP
Gene Synonym:
Exon (7)993..1155
Gene:TYMP
Gene Synonym:
Exon (8)1156..1386
Gene:TYMP
Gene Synonym:
Exon (9)1387..1527
Gene:TYMP
Gene Synonym:
Exon (10)1528..1694
Gene:TYMP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001257988
Organism Homo sapiens (human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001257988

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23077
Clone ID Related Accession (Same CDS sequence) NM_001257989
Accession Version NM_001257989.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1464bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thymidine phosphorylase isoform 2 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, AK225269.1, BC052211.2 and BM744178.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) uses alternate splice sites in the 5' UTR and the 3' coding region, compared to variant 1. The resulting isoform (2) has an additional segment in the C-terminal region, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK225269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGC CCCTCTCCCC GCAGGCACCG TGGAGCTGGT CCGGGCGCTG
CCGCTGGCGC TGGTGCTGCA CGAGCTCGGG GCCGGGCGCA GCCGCGCTGG GGAGCCGCTC
CGCCTGGGGG TGGGCGCAGA GCTGCTGGTC GACGTGGGTC AGAGGCTGCG CCGTGGGACC
CCCTGGCTCC GCGTGCACCG GGACGGCCCC GCGCTCAGCG GCCCGCAGAG CCGCGCCCTG
CAGGAGGCGC TCGTACTCTC CGACCGCGCG CCATTCGCCG CCCCCTCGCC CTTCGCAGAG
CTCGTTCTGC CGCCGCAGCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244918.1
CDS193..1656
Misc Feature(1)97..99(+)
Misc Feature(2)295..489(+)
Misc Feature(3)301..1545(+)
Misc Feature(4)517..1212(+)
Misc Feature(5)985..1029(+)
Misc Feature(6)1177..1218(+)
Misc Feature(7)1372..1593(+)
Misc Feature(8)1384..1410(+)
Misc Feature(9)1564..1590(+)
Exon (1)1..182
Gene:TYMP
Gene Synonym:
Exon (2)183..406
Gene:TYMP
Gene Synonym:
Exon (3)407..609
Gene:TYMP
Gene Synonym:
Exon (4)610..708
Gene:TYMP
Gene Synonym:
Exon (5)709..838
Gene:TYMP
Gene Synonym:
Exon (6)839..957
Gene:TYMP
Gene Synonym:
Exon (7)958..1120
Gene:TYMP
Gene Synonym:
Exon (8)1121..1351
Gene:TYMP
Gene Synonym:
Exon (9)1352..1507
Gene:TYMP
Gene Synonym:
Exon (10)1508..1674
Gene:TYMP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001257989
Organism Homo sapiens (human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001257989

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGC CCCTCTCCCC GCAGGCACCG TGGAGCTGGT CCGGGCGCTG
CCGCTGGCGC TGGTGCTGCA CGAGCTCGGG GCCGGGCGCA GCCGCGCTGG GGAGCCGCTC
CGCCTGGGGG TGGGCGCAGA GCTGCTGGTC GACGTGGGTC AGAGGCTGCG CCGTGGGACC
CCCTGGCTCC GCGTGCACCG GGACGGCCCC GCGCTCAGCG GCCCGCAGAG CCGCGCCCTG
CAGGAGGCGC TCGTACTCTC CGACCGCGCG CCATTCGCCG CCCCCTCGCC CTTCGCAGAG
CTCGTTCTGC CGCCGCAGCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113755 , NM_001113756 , NM_001257988 , NM_001953
Accession Version NM_001113755.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thymidine phosphorylase isoform 1 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, AK314716.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced gi:166158921. Transcript Variant: This variant (1) and variants 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK314716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107227.1
CDS205..1653
Misc Feature(1)97..99(+)
Misc Feature(2)307..501(+)
Misc Feature(3)313..1542(+)
Misc Feature(4)529..1224(+)
Misc Feature(5)997..1041(+)
Misc Feature(6)1189..1230(+)
Misc Feature(7)1369..1590(+)
Misc Feature(8)1381..1407(+)
Misc Feature(9)1561..1587(+)
Exon (1)1..182
Gene:TYMP
Gene Synonym:
Exon (2)183..418
Gene:TYMP
Gene Synonym:
Exon (3)419..621
Gene:TYMP
Gene Synonym:
Exon (4)622..720
Gene:TYMP
Gene Synonym:
Exon (5)721..850
Gene:TYMP
Gene Synonym:
Exon (6)851..969
Gene:TYMP
Gene Synonym:
Exon (7)970..1132
Gene:TYMP
Gene Synonym:
Exon (8)1133..1363
Gene:TYMP
Gene Synonym:
Exon (9)1364..1504
Gene:TYMP
Gene Synonym:
Exon (10)1505..1671
Gene:TYMP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001113755
Organism Homo sapiens (human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113755

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Thymidine phosphorylase expression in B-cell lymphomas and its significance: a new prognostic marker?
Anal. Quant. Cytol. Histol. 35 (6), 301-305 (2013)
Nie,X., Clifford,P.M., Bhat,R., Heintzelman,R., Abraham,M. and Hou,J.S.


book

A polymorphism in the TYMP gene is associated with the outcome of HLA-identical sibling allogeneic stem cell transplantation
Am. J. Hematol. 88 (10), 883-889 (2013)
Guillem V, Hernandez-Boluda JC, Gallardo D, Buno I, Bosch A, Martinez-Laperche C, de la Camara R, Brunet S, Martin C, Nieto JB, Martinez C, Perez A, Montoro J, Garcia-Noblejas A and Solano C.


book

[Expression of vascular growth factors in intestinal tissues in colorectal carcinoma patients with schistosomiasis japonica]
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 25 (3), 250-254 (2013)
Ruan SL, Wang B, Lu QM, Dong LR, Cao CX, Xu SL and Shen WY.


book

Interferon-alpha enhances 5'-deoxy-5-fluorouridine-induced apoptosis by ERK-dependant upregulation of thymidine phosphorylase
Biomed Res Int 2013, 132793 (2013)
Zhu Y, Xu L, Fan Y, Li C, Zhang Y, Zheng H, Hou K, Qu X and Liu Y.


book

Seventy-five genetic loci influencing the human red blood cell
Nature 492 (7429), 369-375 (2012)
van der Harst P, Zhang W, Mateo Leach I, Rendon A, Verweij N, Sehmi J, Paul DS, Elling U, Allayee H, Li X, Radhakrishnan A, Tan ST, Voss K, Weichenberger CX, Albers CA, Al-Hussani A, Asselbergs FW, Ciullo M, Danjou F, Dina C, Esko T, Evans DM, Franke L, Gogele M, Hartiala J, Hersch M, Holm H, Hottenga JJ, Kanoni S, Kleber ME, Lagou V, Langenberg C, Lopez LM, Lyytikainen LP, Melander O, Murgia F, Nolte IM, O'Reilly PF, Padmanabhan S, Parsa A, Pirastu N, Porcu E, Portas L, Prokopenko I, Ried JS, Shin SY, Tang CS, Teumer A, Traglia M, Ulivi S, Westra HJ, Yang J, Zhao JH, Anni F, Abdellaoui A, Attwood A, Balkau B, Bandinelli S, Bastardot F, Benyamin B, Boehm BO, Cookson WO, Das D, de Bakker PI, de Boer RA, de Geus EJ, de Moor MH, Dimitriou M, Domingues FS, Doring A, Engstrom G, Eyjolfsson GI, Ferrucci L, Fischer K, Galanello R, Garner SF, Genser B, Gibson QD, Girotto G, Gudbjartsson DF, Harris SE, Hartikainen AL, Hastie CE, Hedblad B, Illig T, Jolley J, Kahonen M, Kema IP, Kemp JP, Liang L, Lloyd-Jones H, Loos RJ, Meacham S, Medland SE, Meisinger C, Memari Y, Mihailov E, Miller K, Moffatt MF, Nauck M, Novatchkova M, Nutile T, Olafsson I, Onundarson PT, Parracciani D, Penninx BW, Perseu L, Piga A, Pistis G, Pouta A, Puc U, Raitakari O, Ring SM, Robino A, Ruggiero D, Ruokonen A, Saint-Pierre A, Sala C, Salumets A, Sambrook J, Schepers H, Schmidt CO, Sillje HH, Sladek R, Smit JH, Starr JM, Stephens J, Sulem P, Tanaka T, Thorsteinsdottir U, Tragante V, van Gilst WH, van Pelt LJ, van Veldhuisen DJ, Volker U, Whitfield JB, Willemsen G, Winkelmann BR, Wirnsberger G, Algra A, Cucca F, d'Adamo AP, Danesh J, Deary IJ, Dominiczak AF, Elliott P, Fortina P, Froguel P, Gasparini P, Greinacher A, Hazen SL, Jarvelin MR, Khaw KT, Lehtimaki T, Maerz W, Martin NG, Metspalu A, Mitchell BD, Montgomery GW, Moore C, Navis G, Pirastu M, Pramstaller PP, Ramirez-Solis R, Schadt E, Scott J, Shuldiner AR, Smith GD, Smith JG, Snieder H, Sorice R, Spector TD, Stefansson K, Stumvoll M, Tang WH, Toniolo D, Tonjes A, Visscher PM, Vollenweider P, Wareham NJ, Wolffenbuttel BH, Boomsma DI, Beckmann JS, Dedoussis GV, Deloukas P, Ferreira MA, Sanna S, Uda M, Hicks AA, Penninger JM, Gieger C, Kooner JS, Ouwehand WH, Soranzo N and Chambers JC.


book

Mitochondrial Neurogastrointestinal Encephalopathy Disease
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Shoffner,J.M.


book

Neurotrophic action of gliostatin on cortical neurons. Identity of gliostatin and platelet-derived endothelial cell growth factor
J. Biol. Chem. 267 (28), 20311-20316 (1992)
Asai K, Nakanishi K, Isobe I, Eksioglu YZ, Hirano A, Hama K, Miyamoto T and Kato T.


book

Platelet-derived endothelial cell growth factor has thymidine phosphorylase activity
Biochem. Biophys. Res. Commun. 184 (3), 1311-1316 (1992)
Usuki K, Saras J, Waltenberger J, Miyazono K, Pierce G, Thomason A and Heldin CH.


book

Angiogenic factor
Nature 356 (6371), 668 (1992)
Furukawa,T., Yoshimura,A., Sumizawa,T., Haraguchi,M., Akiyama,S., Fukui,K., Ishizawa,M. and Yamada,Y.


book

Regional localization of the human platelet-derived endothelial cell growth factor (ECGF1) gene to chromosome 22q13
Cytogenet. Cell Genet. 59 (1), 22-23 (1992)
Stenman G, Sahlin P, Dumanski JP, Hagiwara K, Ishikawa F, Miyazono K, Collins VP and Heldin CH.


 
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