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EDN3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EDN3
Entrez Gene ID 1908
Full Name endothelin 3
Synonyms ET-3, ET3, HSCR4, PPET3, WS4B
General protein information
Preferred Names
endothelin-3
Names
endothelin-3
preproendothelin-3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q13.2-q13.3

Summary The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]. lac of sum
Disorder MIM:

131242

Disorder Html: Waardenburg syndrome, type 4B, 613265 (3); Central hypoventilation

mRNA and Protein(s)

mRNA Protein Name
XM_005260312 XP_005260369 endothelin-3 isoform X1
XM_011528655 XP_011526957 endothelin-3 isoform X2
XM_005260313 XP_005260370 endothelin-3 isoform X3
XM_006723734 XP_006723797 endothelin-3 isoform X4
NM_207034 NP_996917 endothelin-3 isoform 1 preproprotein
NM_207033 NP_996916 endothelin-3 isoform 3 preproprotein
NM_207032 NP_996915 endothelin-3 isoform 2 preproprotein
NM_001302455 NP_001289384 endothelin-3 isoform 4 preproprotein
NM_001302456 NP_001289385 endothelin-3 isoform 5 preproprotein



Homo sapiens (human) EDN3 NP_996915.1
Pan troglodytes (chimpanzee) EDN3 XP_525373.2
Canis lupus familiaris (dog) EDN3 NP_001002942.1
Bos taurus (cattle) EDN3 NP_001095449.1
Mus musculus (house mouse) Edn3 NP_031929.1
Rattus norvegicus (Norway rat) Edn3 NP_001071118.1
Gallus gallus (chicken) EDN3 XP_001231488.3


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EDN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu52927 XM_005260312 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu57426 XM_011528655 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu52928 XM_005260313 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu52929 XM_006723734 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu16242 NM_207034 Homo sapiens endothelin 3 (EDN3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
OHu16314 NM_207033 Homo sapiens endothelin 3 (EDN3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu16162 NM_207032 Homo sapiens endothelin 3 (EDN3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu57427 NM_001302455 Homo sapiens endothelin 3 (EDN3), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
OHu52930 NM_001302456 Homo sapiens endothelin 3 (EDN3), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu52927
Clone ID Related Accession (Same CDS sequence) XM_005260312
Accession Version XM_005260312.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 756bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835839. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGACAGGTT GAAGTCAAGG ACCAACAAAG CAAGCAGGCT
TTAGACCTCC ACCATCCAAA GCTCATGCCC GGCAGTGGAC TCGCCCTCGC TCCATCTACC
TGCCCCCGCT GCCTCTTTCA GGAAGGAGCC CCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260369.1
CDS390..1145
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation

Target ORF information:

RefSeq Version XM_005260312
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260312

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGACAGGTT GAAGTCAAGG ACCAACAAAG CAAGCAGGCT
TTAGACCTCC ACCATCCAAA GCTCATGCCC GGCAGTGGAC TCGCCCTCGC TCCATCTACC
TGCCCCCGCT GCCTCTTTCA GGAAGGAGCC CCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57426
Clone ID Related Accession (Same CDS sequence) XM_011528655
Accession Version XM_011528655.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526957.1
CDS390..1124
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation

Target ORF information:

RefSeq Version XM_011528655
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528655

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52928
Clone ID Related Accession (Same CDS sequence) XM_005260313
Accession Version XM_005260313.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835840. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGGTTGAAGT CAAGGACCAA CAAAGCAAGC AGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260370.1
CDS390..1028
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation

Target ORF information:

RefSeq Version XM_005260313
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260313

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGGTTGAAGT CAAGGACCAA CAAAGCAAGC AGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52929
Clone ID Related Accession (Same CDS sequence) XM_006723734
Accession Version XM_006723734.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835841. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723797.1
CDS390..1016
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation

Target ORF information:

RefSeq Version XM_006723734
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006723734

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16242
Clone ID Related Accession (Same CDS sequence) NM_207034
Accession Version NM_207034.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC008876.2. This sequence is a reference standard in the RefSeqGene project. On Oct 22, 2014 this sequence version replaced gi:46370063. Transcript Variant: This variant (4) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008876.2, J05081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT TGAAGTCAAG
GACCAACAAA GCAAGCAGGC TTTAGACCTC CACCATCCAA AGCTCATGCC CGGCAGTGGA
CTCGCCCTCG CTCCATCTAC CTGCCCCCGC TGCCTCTTTC AGGAAGGAGC CCCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_996917.1
CDS387..1103
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..740(+)
Misc Feature(4)735..737(+)
Misc Feature(5)765..767(+)
Misc Feature(6)858..923(+)
Misc Feature(7)861..905(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..974
Gene:EDN3
Gene Synonym:
Exon (5)975..2636
Gene:EDN3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207034
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207034

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT TGAAGTCAAG
GACCAACAAA GCAAGCAGGC TTTAGACCTC CACCATCCAA AGCTCATGCC CGGCAGTGGA
CTCGCCCTCG CTCCATCTAC CTGCCCCCGC TGCCTCTTTC AGGAAGGAGC CCCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16314
Clone ID Related Accession (Same CDS sequence) NM_207033
Accession Version NM_207033.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1, X52001.1 and BC008876.2. On Oct 22, 2014 this sequence version replaced gi:46370061. Transcript Variant: This variant (3) lacks an exon in the 3' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 4. The encoded isoform (3) is shorter and has two distinct internal amino acids, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52001.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_996916.1
CDS387..1061
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..737(+)
Misc Feature(4)765..767(+)
Misc Feature(5)858..923(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..2594
Gene:EDN3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207033
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207033

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16162
Clone ID Related Accession (Same CDS sequence) NM_207032
Accession Version NM_207032.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1 and BC008876.2. On Oct 22, 2014 this sequence version replaced gi:46370059. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 4. The encoded isoform (2), is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTTGAAG TCAAGGACCA ACAAAGCAAG CAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_996915.1
CDS387..1046
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..740(+)
Misc Feature(4)735..737(+)
Misc Feature(5)765..767(+)
Misc Feature(6)858..923(+)
Misc Feature(7)861..905(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..1009
Gene:EDN3
Gene Synonym:
Exon (5)1010..2671
Gene:EDN3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207032
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207032

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTTGAAG TCAAGGACCA ACAAAGCAAG CAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57427
Clone ID Related Accession (Same CDS sequence) NM_001302455
Accession Version NM_001302455.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 4 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1, DN989853.1 and BC008876.2. Transcript Variant: This variant (5) uses an alternate splice site in the 3' terminal exon, which results in a frameshift and an early stop codon, compared to variant 4. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN989853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGAC AGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289384.1
CDS387..983
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..740(+)
Misc Feature(4)858..923(+)
Misc Feature(5)861..905(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..974
Gene:EDN3
Gene Synonym:
Exon (5)975..2640
Gene:EDN3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302455
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302455

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGAC AGGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52930
Clone ID Related Accession (Same CDS sequence) NM_001302456
Accession Version NM_001302456.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 579bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 5 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1, DQ096281.1, X52001.1 and BC008876.2. On Oct 22, 2014 this sequence version replaced gi:578835843. Transcript Variant: This variant (6) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 4. The encoded isoform (5) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ096281.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGGTTGAAGT CAAGGACCAA CAAAGCAAGC AGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289385.1
CDS387..965
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)858..923(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..2590
Gene:EDN3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302456
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302456

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGGTTGAAGT CAAGGACCAA CAAAGCAAGC AGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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