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EDN3 endothelin 3 [Homo sapiens (human)]

Gene Symbol EDN3
Entrez Gene ID 1908
Full Name endothelin 3
Synonyms ET-3, ET3, HSCR4, PPET3, WS4B
General protein information
Preferred Names
endothelin-3
Names
endothelin-3
preproendothelin-3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q13.2-q13.3

Summary The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]. lac of sum
Disorder MIM:

131242

Disorder Html: Waardenburg syndrome, type 4B, 613265 (3); Central hypoventilation





The following EDN3 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDN3 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu52927 XM_005260312 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu57426 XM_011528655 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu52928 XM_005260313 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu52929 XM_006723734 PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu16242 NM_207034 Homo sapiens endothelin 3 (EDN3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $269
OHu16314 NM_207033 Homo sapiens endothelin 3 (EDN3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu16162 NM_207032 Homo sapiens endothelin 3 (EDN3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu57427 NM_001302455 Homo sapiens endothelin 3 (EDN3), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $99
OHu52930 NM_001302456 Homo sapiens endothelin 3 (EDN3), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu52927
Accession Version XM_005260312.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 756bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835839. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGACAGGTT GAAGTCAAGG ACCAACAAAG CAAGCAGGCT
TTAGACCTCC ACCATCCAAA GCTCATGCCC GGCAGTGGAC TCGCCCTCGC TCCATCTACC
TGCCCCCGCT GCCTCTTTCA GGAAGGAGCC CCTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005260369.1
CDS390..1145
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation
Position Chain Variation Link
27 27 c, g dbSNP:538954140
76 76 g, t dbSNP:149334
98 98 a, g dbSNP:553995768
125 125 c, g dbSNP:572327630
142 142 a, g dbSNP:542818479
177 177 c, t dbSNP:11570253
178 178 c, g dbSNP:555127949
197 197 g, t dbSNP:545445596
198 198 c, g dbSNP:573681306
205 205 g, t dbSNP:780505724
226 226 a, g dbSNP:749699689
227 227 c, t dbSNP:544294058
244 244 a, c dbSNP:559509661
267 267 a, g dbSNP:562510121
304 304 a, g dbSNP:755284881
313 313 a, t dbSNP:11570254
329 329 c, t dbSNP:545325392
332 332 c, t dbSNP:777204838
346 346 c, t dbSNP:368814466
349 349 a, c, g, t dbSNP:371551500
350 350 c, t dbSNP:781752092
356 356 a, c, t dbSNP:748531162
359 359 g, t dbSNP:527501488
367 367 a, g dbSNP:749491983
371 371 a, c dbSNP:375594972
372 372 a, g dbSNP:774507126
374 374 c, g dbSNP:759572315
375 375 c, g dbSNP:764022228
377 377 c, g dbSNP:776492495
384 384 a, g dbSNP:761651395
385 385 a, g dbSNP:149697
399 399 a, g dbSNP:567434377
400 400 c, g dbSNP:202140125
408 408 c, t dbSNP:758047604
413 413 g, t dbSNP:765898330
421 421 g, t dbSNP:751116353
424 424 c, t dbSNP:754475513
431 431 c, t dbSNP:781768805
432 432 g, t dbSNP:374697035
437 437 c, t dbSNP:550205592
438 438 a, g, t dbSNP:11570255
441 441 a, g dbSNP:457755
446 446 a, c, t dbSNP:532453805
450 450 c, t dbSNP:747041914
451 451 c, g dbSNP:769802593
469 469 a, c dbSNP:772960457
472 472 c, t dbSNP:762824805
480 480 a, c dbSNP:770621680
482 482 c, t dbSNP:774217144
483 483 a, g dbSNP:369335129
487 487 a, c, t dbSNP:371691396
491 491 a, c, t dbSNP:760033782
493 493 a, g dbSNP:754287022
496 496 c, t dbSNP:757704427
499 499 a, c dbSNP:779215514
506 506 a, c dbSNP:750694303
510 510 a, g, t dbSNP:758644212
511 511 g, t dbSNP:747096907
519 519 a, g dbSNP:761584084
524 524 c, t dbSNP:143959111
526 526 a, g dbSNP:777826972
530 530 a, g dbSNP:113842164
533 533 c, g dbSNP:749180844
535 535 c, t dbSNP:148646322
548 548 c, t dbSNP:770806114
552 552 a, g dbSNP:774307682
556 556 a, g dbSNP:369619472
568 568 c, g dbSNP:745615264
570 570 c, t dbSNP:373609215
576 576 a, g dbSNP:775223561
579 579 a, g dbSNP:760290378
580 580 a, g dbSNP:457651
581 581 g, t dbSNP:777233311
582 582 a, g dbSNP:762206123
589 589 -, tgg dbSNP:748397146
591 591 c, t dbSNP:765686063
592 592 -, t dbSNP:772230072
592 592 c, t dbSNP:750818786
594 594 a, g dbSNP:758697348
595 595 c, t dbSNP:766605338
597 597 a, g dbSNP:369429604
607 607 a, g dbSNP:376041123
611 611 a, c dbSNP:781103592
614 614 c, t dbSNP:565731779
623 623 c, t dbSNP:757170661
628 628 a, g dbSNP:778923787
633 633 a, g dbSNP:370091378
640 640 a, c, t dbSNP:142051051
643 643 c, g, t dbSNP:150731720
645 645 a, g dbSNP:776028645
646 646 a, t dbSNP:761348961
649 649 g, t dbSNP:765738929
650 650 a, g dbSNP:139978625
651 651 g, t dbSNP:763369291
654 654 c, t dbSNP:144937074
655 655 c, t dbSNP:751850788
657 657 c, g dbSNP:147917971
659 659 a, g dbSNP:767736650
666 666 c, g dbSNP:267606779
667 667 a, g dbSNP:752720292
672 672 a, g dbSNP:756201310
676 676 a, g dbSNP:141776410
682 682 a, c dbSNP:745795470
683 683 c, g dbSNP:145414258
690 690 a, g dbSNP:779690794
691 691 c, t dbSNP:746771631
695 695 c, g dbSNP:768226122
698 698 a, g dbSNP:780830178
712 712 c, t dbSNP:747625344
719 719 c, t dbSNP:769283989
721 721 a, g dbSNP:773779627
724 724 a, g dbSNP:267606778
725 725 c, g dbSNP:763421771
729 729 a, g dbSNP:771336824
734 734 c, t dbSNP:149729435
739 739 c, g dbSNP:774607474
745 745 -, ac dbSNP:776300941
745 745 a, t dbSNP:202126740
749 749 c, t dbSNP:759875525
750 750 c, g dbSNP:767643055
751 751 c, g, t dbSNP:267606028
761 761 a, g dbSNP:781011983
762 762 g, t dbSNP:34604265
769 769 a, g dbSNP:752400458
780 780 a, g dbSNP:755789488
782 782 c, g, t dbSNP:777336048
793 793 g, t dbSNP:374873073
799 799 a, g, t dbSNP:779352707
803 803 c, t dbSNP:772369425
814 814 c, t dbSNP:536097197
815 815 a, g dbSNP:187049336
832 832 a, g dbSNP:769005371
834 834 a, c dbSNP:776936096
840 840 c, t dbSNP:762043350
844 844 c, t dbSNP:765355566
847 847 a, g, t dbSNP:774197901
849 849 c, t dbSNP:767312223
850 850 a, g dbSNP:145239588
855 855 c, t dbSNP:755843195
859 859 c, t dbSNP:369295391
861 861 a, c dbSNP:753482233
863 863 c, t dbSNP:756755957
865 865 g, t dbSNP:74315384
866 866 c, t dbSNP:149177260
867 867 a, g dbSNP:778461734
869 869 c, t dbSNP:745314073
873 873 c, g dbSNP:758974269
875 875 a, g, t dbSNP:372385465
884 884 c, t dbSNP:575758752
890 890 a, g dbSNP:143232619
891 891 a, g dbSNP:748374290
896 896 a, c, t dbSNP:74315385
897 897 c, t dbSNP:151250216
899 899 a, g dbSNP:779957841
914 914 a, g dbSNP:34780366
923 923 c, t dbSNP:374020215
924 924 a, g dbSNP:767453373
931 931 g, t dbSNP:576523143
943 943 c, t dbSNP:368327225
944 944 a, g dbSNP:770991287
948 948 -, a dbSNP:11570344
954 954 -, a dbSNP:397843910
956 956 -, ag dbSNP:769685879
961 961 -, aag dbSNP:775484472
963 963 c, g dbSNP:775443105
974 974 c, g dbSNP:760582150
977 977 a, g dbSNP:768529772
996 996 a, g dbSNP:776520707
999 999 c, g, t dbSNP:761447718
1013 1013 a, g dbSNP:748824746
1018 1018 g, t dbSNP:757499758
1023 1023 a, g dbSNP:779040805
1027 1027 a, t dbSNP:145582774
1028 1028 a, g dbSNP:745960429
1031 1031 c, t dbSNP:758459588
1055 1055 -, cct dbSNP:761573030
1058 1058 a, c dbSNP:781257967
1079 1079 a, c, t dbSNP:748005608
1080 1080 a, c, g dbSNP:772906477
1083 1083 a, c dbSNP:532030990
1091 1091 c, t dbSNP:137985872
1092 1092 a, g dbSNP:201352056
1097 1097 c, t dbSNP:370508486
1098 1098 a, g dbSNP:11570351
1099 1099 c, t dbSNP:761106361
1101 1101 c, g dbSNP:764620452
1103 1103 a, g dbSNP:754204521
1105 1105 c, t dbSNP:200741552
1113 1113 c, t dbSNP:757559436
1115 1115 c, t dbSNP:765500226
1116 1116 a, c, t dbSNP:372958987
1117 1117 a, c, g dbSNP:144250756
1125 1125 c, t dbSNP:549116987
1127 1127 c, t dbSNP:756038575
1130 1130 -, g dbSNP:767213063
1131 1131 a, g dbSNP:777702187
1135 1135 c, g dbSNP:749112686
1138 1138 c, t dbSNP:770841292
1139 1139 a, c dbSNP:774182574
1145 1145 a, g dbSNP:376137509
1146 1146 a, g, t dbSNP:138451394
1155 1155 c, t dbSNP:774891807
1159 1159 a, c dbSNP:760163643
1163 1163 a, g dbSNP:370288393
1171 1171 a, c dbSNP:777109038
1173 1173 c, t dbSNP:374488792
1174 1174 a, g dbSNP:368688196
1180 1180 -, c dbSNP:750823872
1181 1181 c, t dbSNP:750694380
1188 1188 a, t dbSNP:567494206
1189 1189 c, t dbSNP:200872063
1192 1192 c, g dbSNP:751597991
1203 1203 a, t dbSNP:463415
1210 1210 g, t dbSNP:555946641
1223 1223 g, t dbSNP:571072081
1226 1226 a, c dbSNP:538768879
1238 1238 a, t dbSNP:553876011
1285 1285 -, cc dbSNP:752653775
1285 1285 -, c dbSNP:11475273
1286 1286 -, c dbSNP:754876648
1287 1287 c, g dbSNP:372569173
1288 1288 a, c, g, t dbSNP:71321536
1290 1290 cc, t dbSNP:377130048
1291 1291 a, c, g, t dbSNP:11570352
1292 1292 a, c dbSNP:576673862
1293 1293 c, g dbSNP:544095029
1294 1294 a, c dbSNP:565501608
1294 1294 -, c dbSNP:397866060
1297 1297 c, t dbSNP:3026576
1310 1310 a, g dbSNP:11570353
1316 1316 a, g dbSNP:3026575
1322 1322 a, c dbSNP:11570354
1324 1324 c, g dbSNP:527379112
1350 1350 a, c dbSNP:548702985
1386 1386 a, g dbSNP:746504168
1463 1463 c, t dbSNP:567211087
1465 1465 g, t dbSNP:533368307
1500 1500 a, g dbSNP:79786751
1590 1590 c, t dbSNP:191496604
1595 1595 c, t dbSNP:546331828
1612 1612 c, g, t dbSNP:143382055
1620 1620 c, t dbSNP:538479580
1622 1622 a, g dbSNP:549881140
1676 1676 a, g dbSNP:11570355
1728 1728 c, t dbSNP:777130895
1740 1740 a, c dbSNP:566044970
1745 1745 a, g dbSNP:3026574
1745 1745 a, g dbSNP:11570356
1782 1782 a, g dbSNP:770384486
1799 1799 c, t dbSNP:778461322
1806 1806 c, g dbSNP:770340747
1853 1853 c, t dbSNP:746433345
1857 1857 a, t dbSNP:775994244
1861 1861 c, g dbSNP:531531144
1901 1901 c, t dbSNP:763311172
1902 1902 a, g dbSNP:57724975
1953 1953 a, c dbSNP:566560322
1997 1997 c, t dbSNP:775734185
2009 2009 c, g dbSNP:3026573
2046 2046 a, g dbSNP:764393299
2059 2059 c, t dbSNP:751714665
2060 2060 c, t dbSNP:11570357
2065 2065 g, t dbSNP:766229089
2067 2067 c, t dbSNP:559330652
2092 2092 c, g dbSNP:753749070
2102 2102 c, t dbSNP:3026572
2107 2107 c, t dbSNP:138371508
2108 2108 a, g dbSNP:559849084
2133 2133 c, t dbSNP:572054658
2151 2151 c, t dbSNP:542670565
2152 2152 c, g dbSNP:13037048
2157 2157 c, t dbSNP:11570358
2158 2158 a, g dbSNP:752457788
2235 2235 c, t dbSNP:181368086
2236 2236 a, g dbSNP:549350852
2268 2268 g, t dbSNP:376536945
2289 2289 c, t dbSNP:11570359
2292 2292 a, g dbSNP:3026571
2298 2298 -, tgtg dbSNP:569486179
2325 2325 c, t dbSNP:777354262
2341 2341 c, t dbSNP:547284575
2400 2400 c, t dbSNP:12322
2401 2401 a, g dbSNP:190002794
2426 2426 c, t dbSNP:746507182
2438 2438 c, t dbSNP:536369470
2441 2441 a, t dbSNP:770418007
2457 2457 a, g dbSNP:554865283
2489 2489 a, g dbSNP:780553745
2585 2585 a, g dbSNP:548692162
2628 2628 a, g dbSNP:183354781
2648 2648 a, c dbSNP:570404603
2650 2650 c, t dbSNP:746455039
2651 2651 c, t dbSNP:6026811
2663 2663 a, g dbSNP:6026812

Target ORF information:

RefSeq Version XM_005260312
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260312

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGACAGGTT GAAGTCAAGG ACCAACAAAG CAAGCAGGCT
TTAGACCTCC ACCATCCAAA GCTCATGCCC GGCAGTGGAC TCGCCCTCGC TCCATCTACC
TGCCCCCGCT GCCTCTTTCA GGAAGGAGCC CCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57426
Accession Version XM_011528655.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011526957.1
CDS390..1124
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation
Position Chain Variation Link
27 27 c, g dbSNP:538954140
76 76 g, t dbSNP:149334
98 98 a, g dbSNP:553995768
125 125 c, g dbSNP:572327630
142 142 a, g dbSNP:542818479
177 177 c, t dbSNP:11570253
178 178 c, g dbSNP:555127949
197 197 g, t dbSNP:545445596
198 198 c, g dbSNP:573681306
205 205 g, t dbSNP:780505724
226 226 a, g dbSNP:749699689
227 227 c, t dbSNP:544294058
244 244 a, c dbSNP:559509661
267 267 a, g dbSNP:562510121
304 304 a, g dbSNP:755284881
313 313 a, t dbSNP:11570254
329 329 c, t dbSNP:545325392
332 332 c, t dbSNP:777204838
346 346 c, t dbSNP:368814466
349 349 a, c, g, t dbSNP:371551500
350 350 c, t dbSNP:781752092
356 356 a, c, t dbSNP:748531162
359 359 g, t dbSNP:527501488
367 367 a, g dbSNP:749491983
371 371 a, c dbSNP:375594972
372 372 a, g dbSNP:774507126
374 374 c, g dbSNP:759572315
375 375 c, g dbSNP:764022228
377 377 c, g dbSNP:776492495
384 384 a, g dbSNP:761651395
385 385 a, g dbSNP:149697
399 399 a, g dbSNP:567434377
400 400 c, g dbSNP:202140125
408 408 c, t dbSNP:758047604
413 413 g, t dbSNP:765898330
421 421 g, t dbSNP:751116353
424 424 c, t dbSNP:754475513
431 431 c, t dbSNP:781768805
432 432 g, t dbSNP:374697035
437 437 c, t dbSNP:550205592
438 438 a, g, t dbSNP:11570255
441 441 a, g dbSNP:457755
446 446 a, c, t dbSNP:532453805
450 450 c, t dbSNP:747041914
451 451 c, g dbSNP:769802593
469 469 a, c dbSNP:772960457
472 472 c, t dbSNP:762824805
480 480 a, c dbSNP:770621680
482 482 c, t dbSNP:774217144
483 483 a, g dbSNP:369335129
487 487 a, c, t dbSNP:371691396
491 491 a, c, t dbSNP:760033782
493 493 a, g dbSNP:754287022
496 496 c, t dbSNP:757704427
499 499 a, c dbSNP:779215514
506 506 a, c dbSNP:750694303
510 510 a, g, t dbSNP:758644212
511 511 g, t dbSNP:747096907
519 519 a, g dbSNP:761584084
524 524 c, t dbSNP:143959111
526 526 a, g dbSNP:777826972
530 530 a, g dbSNP:113842164
533 533 c, g dbSNP:749180844
535 535 c, t dbSNP:148646322
548 548 c, t dbSNP:770806114
552 552 a, g dbSNP:774307682
556 556 a, g dbSNP:369619472
568 568 c, g dbSNP:745615264
570 570 c, t dbSNP:373609215
576 576 a, g dbSNP:775223561
579 579 a, g dbSNP:760290378
580 580 a, g dbSNP:457651
581 581 g, t dbSNP:777233311
582 582 a, g dbSNP:762206123
589 589 -, tgg dbSNP:748397146
591 591 c, t dbSNP:765686063
592 592 -, t dbSNP:772230072
592 592 c, t dbSNP:750818786
594 594 a, g dbSNP:758697348
595 595 c, t dbSNP:766605338
597 597 a, g dbSNP:369429604
607 607 a, g dbSNP:376041123
611 611 a, c dbSNP:781103592
614 614 c, t dbSNP:565731779
623 623 c, t dbSNP:757170661
628 628 a, g dbSNP:778923787
633 633 a, g dbSNP:370091378
640 640 a, c, t dbSNP:142051051
643 643 c, g, t dbSNP:150731720
645 645 a, g dbSNP:776028645
646 646 a, t dbSNP:761348961
649 649 g, t dbSNP:765738929
650 650 a, g dbSNP:139978625
651 651 g, t dbSNP:763369291
654 654 c, t dbSNP:144937074
655 655 c, t dbSNP:751850788
657 657 c, g dbSNP:147917971
659 659 a, g dbSNP:767736650
666 666 c, g dbSNP:267606779
667 667 a, g dbSNP:752720292
672 672 a, g dbSNP:756201310
676 676 a, g dbSNP:141776410
682 682 a, c dbSNP:745795470
683 683 c, g dbSNP:145414258
690 690 a, g dbSNP:779690794
691 691 c, t dbSNP:746771631
695 695 c, g dbSNP:768226122
698 698 a, g dbSNP:780830178
712 712 c, t dbSNP:747625344
719 719 c, t dbSNP:769283989
721 721 a, g dbSNP:773779627
724 724 a, g dbSNP:267606778
725 725 c, g dbSNP:763421771
729 729 a, g dbSNP:771336824
734 734 c, t dbSNP:149729435
739 739 c, g dbSNP:774607474
745 745 -, ac dbSNP:776300941
745 745 a, t dbSNP:202126740
749 749 c, t dbSNP:759875525
750 750 c, g dbSNP:767643055
751 751 c, g, t dbSNP:267606028
761 761 a, g dbSNP:781011983
762 762 g, t dbSNP:34604265
769 769 a, g dbSNP:752400458
780 780 a, g dbSNP:755789488
782 782 c, g, t dbSNP:777336048
793 793 g, t dbSNP:374873073
799 799 a, g, t dbSNP:779352707
803 803 c, t dbSNP:772369425
814 814 c, t dbSNP:536097197
815 815 a, g dbSNP:187049336
832 832 a, g dbSNP:769005371
834 834 a, c dbSNP:776936096
840 840 c, t dbSNP:762043350
844 844 c, t dbSNP:765355566
847 847 a, g, t dbSNP:774197901
849 849 c, t dbSNP:767312223
850 850 a, g dbSNP:145239588
855 855 c, t dbSNP:755843195
859 859 c, t dbSNP:369295391
861 861 a, c dbSNP:753482233
863 863 c, t dbSNP:756755957
865 865 g, t dbSNP:74315384
866 866 c, t dbSNP:149177260
867 867 a, g dbSNP:778461734
869 869 c, t dbSNP:745314073
873 873 c, g dbSNP:758974269
875 875 a, g, t dbSNP:372385465
884 884 c, t dbSNP:575758752
890 890 a, g dbSNP:143232619
891 891 a, g dbSNP:748374290
896 896 a, c, t dbSNP:74315385
897 897 c, t dbSNP:151250216
899 899 a, g dbSNP:779957841
914 914 a, g dbSNP:34780366
923 923 c, t dbSNP:374020215
924 924 a, g dbSNP:767453373
931 931 g, t dbSNP:576523143
933 933 g, t dbSNP:541847608
934 934 c, t dbSNP:368692180
942 942 a, t dbSNP:770090736
943 943 c, g dbSNP:372324305
957 957 c, t dbSNP:368327225
958 958 a, g dbSNP:770991287
962 962 -, a dbSNP:11570344
968 968 -, a dbSNP:397843910
970 970 -, ag dbSNP:769685879
975 975 -, aag dbSNP:775484472
977 977 c, g dbSNP:775443105
988 988 c, g dbSNP:760582150
991 991 a, g dbSNP:768529772
992 992 a, g dbSNP:748824746
997 997 g, t dbSNP:757499758
1002 1002 a, g dbSNP:779040805
1006 1006 a, t dbSNP:145582774
1007 1007 a, g dbSNP:745960429
1010 1010 c, t dbSNP:758459588
1034 1034 -, cct dbSNP:761573030
1037 1037 a, c dbSNP:781257967
1058 1058 a, c, t dbSNP:748005608
1059 1059 a, c, g dbSNP:772906477
1062 1062 a, c dbSNP:532030990
1070 1070 c, t dbSNP:137985872
1071 1071 a, g dbSNP:201352056
1076 1076 c, t dbSNP:370508486
1077 1077 a, g dbSNP:11570351
1078 1078 c, t dbSNP:761106361
1080 1080 c, g dbSNP:764620452
1082 1082 a, g dbSNP:754204521
1084 1084 c, t dbSNP:200741552
1092 1092 c, t dbSNP:757559436
1094 1094 c, t dbSNP:765500226
1095 1095 a, c, t dbSNP:372958987
1096 1096 a, c, g dbSNP:144250756
1104 1104 c, t dbSNP:549116987
1106 1106 c, t dbSNP:756038575
1109 1109 -, g dbSNP:767213063
1110 1110 a, g dbSNP:777702187
1114 1114 c, g dbSNP:749112686
1117 1117 c, t dbSNP:770841292
1118 1118 a, c dbSNP:774182574
1124 1124 a, g dbSNP:376137509
1125 1125 a, g, t dbSNP:138451394
1134 1134 c, t dbSNP:774891807
1138 1138 a, c dbSNP:760163643
1142 1142 a, g dbSNP:370288393
1150 1150 a, c dbSNP:777109038
1152 1152 c, t dbSNP:374488792
1153 1153 a, g dbSNP:368688196
1159 1159 -, c dbSNP:750823872
1160 1160 c, t dbSNP:750694380
1167 1167 a, t dbSNP:567494206
1168 1168 c, t dbSNP:200872063
1171 1171 c, g dbSNP:751597991
1182 1182 a, t dbSNP:463415
1189 1189 g, t dbSNP:555946641
1202 1202 g, t dbSNP:571072081
1205 1205 a, c dbSNP:538768879
1217 1217 a, t dbSNP:553876011
1264 1264 -, cc dbSNP:752653775
1264 1264 -, c dbSNP:11475273
1265 1265 -, c dbSNP:754876648
1266 1266 c, g dbSNP:372569173
1267 1267 a, c, g, t dbSNP:71321536
1269 1269 cc, t dbSNP:377130048
1270 1270 a, c, g, t dbSNP:11570352
1271 1271 a, c dbSNP:576673862
1272 1272 c, g dbSNP:544095029
1273 1273 a, c dbSNP:565501608
1273 1273 -, c dbSNP:397866060
1276 1276 c, t dbSNP:3026576
1289 1289 a, g dbSNP:11570353
1295 1295 a, g dbSNP:3026575
1301 1301 a, c dbSNP:11570354
1303 1303 c, g dbSNP:527379112
1329 1329 a, c dbSNP:548702985
1365 1365 a, g dbSNP:746504168
1442 1442 c, t dbSNP:567211087
1444 1444 g, t dbSNP:533368307
1479 1479 a, g dbSNP:79786751
1569 1569 c, t dbSNP:191496604
1574 1574 c, t dbSNP:546331828
1591 1591 c, g, t dbSNP:143382055
1599 1599 c, t dbSNP:538479580
1601 1601 a, g dbSNP:549881140
1655 1655 a, g dbSNP:11570355
1707 1707 c, t dbSNP:777130895
1719 1719 a, c dbSNP:566044970
1724 1724 a, g dbSNP:3026574
1724 1724 a, g dbSNP:11570356
1761 1761 a, g dbSNP:770384486
1778 1778 c, t dbSNP:778461322
1785 1785 c, g dbSNP:770340747
1832 1832 c, t dbSNP:746433345
1836 1836 a, t dbSNP:775994244
1840 1840 c, g dbSNP:531531144
1880 1880 c, t dbSNP:763311172
1881 1881 a, g dbSNP:57724975
1932 1932 a, c dbSNP:566560322
1976 1976 c, t dbSNP:775734185
1988 1988 c, g dbSNP:3026573
2025 2025 a, g dbSNP:764393299
2038 2038 c, t dbSNP:751714665
2039 2039 c, t dbSNP:11570357
2044 2044 g, t dbSNP:766229089
2046 2046 c, t dbSNP:559330652
2071 2071 c, g dbSNP:753749070
2081 2081 c, t dbSNP:3026572
2086 2086 c, t dbSNP:138371508
2087 2087 a, g dbSNP:559849084
2112 2112 c, t dbSNP:572054658
2130 2130 c, t dbSNP:542670565
2131 2131 c, g dbSNP:13037048
2136 2136 c, t dbSNP:11570358
2137 2137 a, g dbSNP:752457788
2214 2214 c, t dbSNP:181368086
2215 2215 a, g dbSNP:549350852
2247 2247 g, t dbSNP:376536945
2268 2268 c, t dbSNP:11570359
2271 2271 a, g dbSNP:3026571
2277 2277 -, tgtg dbSNP:569486179
2304 2304 c, t dbSNP:777354262
2320 2320 c, t dbSNP:547284575
2379 2379 c, t dbSNP:12322
2380 2380 a, g dbSNP:190002794
2405 2405 c, t dbSNP:746507182
2417 2417 c, t dbSNP:536369470
2420 2420 a, t dbSNP:770418007
2436 2436 a, g dbSNP:554865283
2468 2468 a, g dbSNP:780553745
2564 2564 a, g dbSNP:548692162
2607 2607 a, g dbSNP:183354781
2627 2627 a, c dbSNP:570404603
2629 2629 c, t dbSNP:746455039
2630 2630 c, t dbSNP:6026811
2642 2642 a, g dbSNP:6026812

Target ORF information:

RefSeq Version XM_011528655
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528655

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52928
Accession Version XM_005260313.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835840. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGGTTGAAGT CAAGGACCAA CAAAGCAAGC AGGCTTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005260370.1
CDS390..1028
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation
Position Chain Variation Link
27 27 c, g dbSNP:538954140
76 76 g, t dbSNP:149334
98 98 a, g dbSNP:553995768
125 125 c, g dbSNP:572327630
142 142 a, g dbSNP:542818479
177 177 c, t dbSNP:11570253
178 178 c, g dbSNP:555127949
197 197 g, t dbSNP:545445596
198 198 c, g dbSNP:573681306
205 205 g, t dbSNP:780505724
226 226 a, g dbSNP:749699689
227 227 c, t dbSNP:544294058
244 244 a, c dbSNP:559509661
267 267 a, g dbSNP:562510121
304 304 a, g dbSNP:755284881
313 313 a, t dbSNP:11570254
329 329 c, t dbSNP:545325392
332 332 c, t dbSNP:777204838
346 346 c, t dbSNP:368814466
349 349 a, c, g, t dbSNP:371551500
350 350 c, t dbSNP:781752092
356 356 a, c, t dbSNP:748531162
359 359 g, t dbSNP:527501488
367 367 a, g dbSNP:749491983
371 371 a, c dbSNP:375594972
372 372 a, g dbSNP:774507126
374 374 c, g dbSNP:759572315
375 375 c, g dbSNP:764022228
377 377 c, g dbSNP:776492495
384 384 a, g dbSNP:761651395
385 385 a, g dbSNP:149697
399 399 a, g dbSNP:567434377
400 400 c, g dbSNP:202140125
408 408 c, t dbSNP:758047604
413 413 g, t dbSNP:765898330
421 421 g, t dbSNP:751116353
424 424 c, t dbSNP:754475513
431 431 c, t dbSNP:781768805
432 432 g, t dbSNP:374697035
437 437 c, t dbSNP:550205592
438 438 a, g, t dbSNP:11570255
441 441 a, g dbSNP:457755
446 446 a, c, t dbSNP:532453805
450 450 c, t dbSNP:747041914
451 451 c, g dbSNP:769802593
469 469 a, c dbSNP:772960457
472 472 c, t dbSNP:762824805
480 480 a, c dbSNP:770621680
482 482 c, t dbSNP:774217144
483 483 a, g dbSNP:369335129
487 487 a, c, t dbSNP:371691396
491 491 a, c, t dbSNP:760033782
493 493 a, g dbSNP:754287022
496 496 c, t dbSNP:757704427
499 499 a, c dbSNP:779215514
506 506 a, c dbSNP:750694303
510 510 a, g, t dbSNP:758644212
511 511 g, t dbSNP:747096907
519 519 a, g dbSNP:761584084
524 524 c, t dbSNP:143959111
526 526 a, g dbSNP:777826972
530 530 a, g dbSNP:113842164
533 533 c, g dbSNP:749180844
535 535 c, t dbSNP:148646322
548 548 c, t dbSNP:770806114
552 552 a, g dbSNP:774307682
556 556 a, g dbSNP:369619472
568 568 c, g dbSNP:745615264
570 570 c, t dbSNP:373609215
576 576 a, g dbSNP:775223561
579 579 a, g dbSNP:760290378
580 580 a, g dbSNP:457651
581 581 g, t dbSNP:777233311
582 582 a, g dbSNP:762206123
589 589 -, tgg dbSNP:748397146
591 591 c, t dbSNP:765686063
592 592 -, t dbSNP:772230072
592 592 c, t dbSNP:750818786
594 594 a, g dbSNP:758697348
595 595 c, t dbSNP:766605338
597 597 a, g dbSNP:369429604
607 607 a, g dbSNP:376041123
611 611 a, c dbSNP:781103592
614 614 c, t dbSNP:565731779
623 623 c, t dbSNP:757170661
628 628 a, g dbSNP:778923787
633 633 a, g dbSNP:370091378
640 640 a, c, t dbSNP:142051051
643 643 c, g, t dbSNP:150731720
645 645 a, g dbSNP:776028645
646 646 a, t dbSNP:761348961
649 649 g, t dbSNP:765738929
650 650 a, g dbSNP:139978625
651 651 g, t dbSNP:763369291
654 654 c, t dbSNP:144937074
655 655 c, t dbSNP:751850788
657 657 c, g dbSNP:147917971
659 659 a, g dbSNP:767736650
666 666 c, g dbSNP:267606779
667 667 a, g dbSNP:752720292
672 672 a, g dbSNP:756201310
676 676 a, g dbSNP:141776410
682 682 a, c dbSNP:745795470
683 683 c, g dbSNP:145414258
690 690 a, g dbSNP:779690794
691 691 c, t dbSNP:746771631
695 695 c, g dbSNP:768226122
698 698 a, g dbSNP:780830178
712 712 c, t dbSNP:747625344
719 719 c, t dbSNP:769283989
721 721 a, g dbSNP:773779627
724 724 a, g dbSNP:267606778
725 725 c, g dbSNP:763421771
729 729 a, g dbSNP:771336824
734 734 c, t dbSNP:149729435
739 739 c, g dbSNP:774607474
745 745 -, ac dbSNP:776300941
745 745 a, t dbSNP:202126740
749 749 c, t dbSNP:759875525
750 750 c, g dbSNP:767643055
751 751 c, g, t dbSNP:267606028
761 761 a, g dbSNP:781011983
762 762 g, t dbSNP:34604265
769 769 a, g dbSNP:752400458
780 780 a, g dbSNP:755789488
782 782 c, g, t dbSNP:777336048
793 793 g, t dbSNP:374873073
799 799 a, g, t dbSNP:779352707
803 803 c, t dbSNP:772369425
814 814 c, t dbSNP:536097197
815 815 a, g dbSNP:187049336
832 832 a, g dbSNP:769005371
834 834 a, c dbSNP:776936096
840 840 c, t dbSNP:762043350
844 844 c, t dbSNP:765355566
847 847 a, g, t dbSNP:774197901
849 849 c, t dbSNP:767312223
850 850 a, g dbSNP:145239588
855 855 c, t dbSNP:755843195
859 859 c, t dbSNP:369295391
861 861 a, c dbSNP:753482233
863 863 c, t dbSNP:756755957
865 865 g, t dbSNP:74315384
866 866 c, t dbSNP:149177260
867 867 a, g dbSNP:778461734
869 869 c, t dbSNP:745314073
873 873 c, g dbSNP:758974269
875 875 a, g, t dbSNP:372385465
884 884 c, t dbSNP:575758752
890 890 a, g dbSNP:143232619
891 891 a, g dbSNP:748374290
896 896 a, c, t dbSNP:74315385
897 897 c, t dbSNP:151250216
899 899 a, g dbSNP:779957841
914 914 a, g dbSNP:34780366
923 923 c, t dbSNP:374020215
924 924 a, g dbSNP:767453373
931 931 g, t dbSNP:576523143
933 933 g, t dbSNP:541847608
934 934 c, t dbSNP:368692180
942 942 a, t dbSNP:770090736
943 943 c, g dbSNP:372324305
957 957 c, t dbSNP:368327225
958 958 a, g dbSNP:770991287
962 962 -, a dbSNP:11570344
968 968 -, a dbSNP:397843910
970 970 -, ag dbSNP:769685879
975 975 -, aag dbSNP:775484472
977 977 c, g dbSNP:775443105
988 988 c, g dbSNP:760582150
991 991 a, g dbSNP:768529772
993 993 g, t dbSNP:757499758
998 998 a, g dbSNP:779040805
1002 1002 a, t dbSNP:145582774
1003 1003 a, g dbSNP:745960429
1006 1006 c, t dbSNP:758459588
1030 1030 -, cct dbSNP:761573030
1033 1033 a, c dbSNP:781257967
1054 1054 a, c, t dbSNP:748005608
1055 1055 a, c, g dbSNP:772906477
1058 1058 a, c dbSNP:532030990
1066 1066 c, t dbSNP:137985872
1067 1067 a, g dbSNP:201352056
1072 1072 c, t dbSNP:370508486
1073 1073 a, g dbSNP:11570351
1074 1074 c, t dbSNP:761106361
1076 1076 c, g dbSNP:764620452
1078 1078 a, g dbSNP:754204521
1080 1080 c, t dbSNP:200741552
1088 1088 c, t dbSNP:757559436
1090 1090 c, t dbSNP:765500226
1091 1091 a, c, t dbSNP:372958987
1092 1092 a, c, g dbSNP:144250756
1100 1100 c, t dbSNP:549116987
1102 1102 c, t dbSNP:756038575
1105 1105 -, g dbSNP:767213063
1106 1106 a, g dbSNP:777702187
1110 1110 c, g dbSNP:749112686
1113 1113 c, t dbSNP:770841292

Target ORF information:

RefSeq Version XM_005260313
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260313

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGCTCTCTCC CCACAGTAAT TCAAGGACGG CAGAAAAAAC AGACAAAGAA GAGGAAGGGA
AGGTTGAAGT CAAGGACCAA CAAAGCAAGC AGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52929
Accession Version XM_006723734.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578835841. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006723797.1
CDS390..1016
Misc Feature(1)666..755(+)
Misc Feature(2)861..926(+)
Translation
Position Chain Variation Link
27 27 c, g dbSNP:538954140
76 76 g, t dbSNP:149334
98 98 a, g dbSNP:553995768
125 125 c, g dbSNP:572327630
142 142 a, g dbSNP:542818479
177 177 c, t dbSNP:11570253
178 178 c, g dbSNP:555127949
197 197 g, t dbSNP:545445596
198 198 c, g dbSNP:573681306
205 205 g, t dbSNP:780505724
226 226 a, g dbSNP:749699689
227 227 c, t dbSNP:544294058
244 244 a, c dbSNP:559509661
267 267 a, g dbSNP:562510121
304 304 a, g dbSNP:755284881
313 313 a, t dbSNP:11570254
329 329 c, t dbSNP:545325392
332 332 c, t dbSNP:777204838
346 346 c, t dbSNP:368814466
349 349 a, c, g, t dbSNP:371551500
350 350 c, t dbSNP:781752092
356 356 a, c, t dbSNP:748531162
359 359 g, t dbSNP:527501488
367 367 a, g dbSNP:749491983
371 371 a, c dbSNP:375594972
372 372 a, g dbSNP:774507126
374 374 c, g dbSNP:759572315
375 375 c, g dbSNP:764022228
377 377 c, g dbSNP:776492495
384 384 a, g dbSNP:761651395
385 385 a, g dbSNP:149697
399 399 a, g dbSNP:567434377
400 400 c, g dbSNP:202140125
408 408 c, t dbSNP:758047604
413 413 g, t dbSNP:765898330
421 421 g, t dbSNP:751116353
424 424 c, t dbSNP:754475513
431 431 c, t dbSNP:781768805
432 432 g, t dbSNP:374697035
437 437 c, t dbSNP:550205592
438 438 a, g, t dbSNP:11570255
441 441 a, g dbSNP:457755
446 446 a, c, t dbSNP:532453805
450 450 c, t dbSNP:747041914
451 451 c, g dbSNP:769802593
469 469 a, c dbSNP:772960457
472 472 c, t dbSNP:762824805
480 480 a, c dbSNP:770621680
482 482 c, t dbSNP:774217144
483 483 a, g dbSNP:369335129
487 487 a, c, t dbSNP:371691396
491 491 a, c, t dbSNP:760033782
493 493 a, g dbSNP:754287022
496 496 c, t dbSNP:757704427
499 499 a, c dbSNP:779215514
506 506 a, c dbSNP:750694303
510 510 a, g, t dbSNP:758644212
511 511 g, t dbSNP:747096907
519 519 a, g dbSNP:761584084
524 524 c, t dbSNP:143959111
526 526 a, g dbSNP:777826972
530 530 a, g dbSNP:113842164
533 533 c, g dbSNP:749180844
535 535 c, t dbSNP:148646322
548 548 c, t dbSNP:770806114
552 552 a, g dbSNP:774307682
556 556 a, g dbSNP:369619472
568 568 c, g dbSNP:745615264
570 570 c, t dbSNP:373609215
576 576 a, g dbSNP:775223561
579 579 a, g dbSNP:760290378
580 580 a, g dbSNP:457651
581 581 g, t dbSNP:777233311
582 582 a, g dbSNP:762206123
589 589 -, tgg dbSNP:748397146
591 591 c, t dbSNP:765686063
592 592 -, t dbSNP:772230072
592 592 c, t dbSNP:750818786
594 594 a, g dbSNP:758697348
595 595 c, t dbSNP:766605338
597 597 a, g dbSNP:369429604
607 607 a, g dbSNP:376041123
611 611 a, c dbSNP:781103592
614 614 c, t dbSNP:565731779
623 623 c, t dbSNP:757170661
628 628 a, g dbSNP:778923787
633 633 a, g dbSNP:370091378
640 640 a, c, t dbSNP:142051051
643 643 c, g, t dbSNP:150731720
645 645 a, g dbSNP:776028645
646 646 a, t dbSNP:761348961
649 649 g, t dbSNP:765738929
650 650 a, g dbSNP:139978625
651 651 g, t dbSNP:763369291
654 654 c, t dbSNP:144937074
655 655 c, t dbSNP:751850788
657 657 c, g dbSNP:147917971
659 659 a, g dbSNP:767736650
666 666 c, g dbSNP:267606779
667 667 a, g dbSNP:752720292
672 672 a, g dbSNP:756201310
676 676 a, g dbSNP:141776410
682 682 a, c dbSNP:745795470
683 683 c, g dbSNP:145414258
690 690 a, g dbSNP:779690794
691 691 c, t dbSNP:746771631
695 695 c, g dbSNP:768226122
698 698 a, g dbSNP:780830178
712 712 c, t dbSNP:747625344
719 719 c, t dbSNP:769283989
721 721 a, g dbSNP:773779627
724 724 a, g dbSNP:267606778
725 725 c, g dbSNP:763421771
729 729 a, g dbSNP:771336824
734 734 c, t dbSNP:149729435
739 739 c, g dbSNP:774607474
745 745 -, ac dbSNP:776300941
745 745 a, t dbSNP:202126740
749 749 c, t dbSNP:759875525
750 750 c, g dbSNP:767643055
751 751 c, g, t dbSNP:267606028
761 761 a, g dbSNP:781011983
762 762 g, t dbSNP:34604265
769 769 a, g dbSNP:752400458
780 780 a, g dbSNP:755789488
782 782 c, g, t dbSNP:777336048
793 793 g, t dbSNP:374873073
799 799 a, g, t dbSNP:779352707
803 803 c, t dbSNP:772369425
814 814 c, t dbSNP:536097197
815 815 a, g dbSNP:187049336
832 832 a, g dbSNP:769005371
834 834 a, c dbSNP:776936096
840 840 c, t dbSNP:762043350
844 844 c, t dbSNP:765355566
847 847 a, g, t dbSNP:774197901
849 849 c, t dbSNP:767312223
850 850 a, g dbSNP:145239588
855 855 c, t dbSNP:755843195
859 859 c, t dbSNP:369295391
861 861 a, c dbSNP:753482233
863 863 c, t dbSNP:756755957
865 865 g, t dbSNP:74315384
866 866 c, t dbSNP:149177260
867 867 a, g dbSNP:778461734
869 869 c, t dbSNP:745314073
873 873 c, g dbSNP:758974269
875 875 a, g, t dbSNP:372385465
884 884 c, t dbSNP:575758752
890 890 a, g dbSNP:143232619
891 891 a, g dbSNP:748374290
896 896 a, c, t dbSNP:74315385
897 897 c, t dbSNP:151250216
899 899 a, g dbSNP:779957841
914 914 a, g dbSNP:34780366
923 923 c, t dbSNP:374020215
924 924 a, g dbSNP:767453373
931 931 g, t dbSNP:576523143
943 943 c, t dbSNP:368327225
944 944 a, g dbSNP:770991287
948 948 -, a dbSNP:11570344
954 954 -, a dbSNP:397843910
956 956 -, ag dbSNP:769685879
961 961 -, aag dbSNP:775484472
963 963 c, g dbSNP:775443105
974 974 c, g dbSNP:760582150
977 977 a, g dbSNP:768529772
996 996 a, g dbSNP:776520707
999 999 c, g, t dbSNP:761447718
1018 1018 a, g dbSNP:750031027
1023 1023 a, g dbSNP:762593278
1025 1025 c, t dbSNP:200659956
1026 1026 a, g dbSNP:564035567
1028 1028 a, g dbSNP:752163944
1033 1033 g, t dbSNP:114307318
1038 1038 c, t dbSNP:781753009
1039 1039 a, g dbSNP:753113450
1040 1040 a, g dbSNP:374877861
1044 1044 g, t dbSNP:757499758
1049 1049 a, g dbSNP:779040805
1053 1053 a, t dbSNP:145582774
1054 1054 a, g dbSNP:745960429
1057 1057 c, t dbSNP:758459588
1081 1081 -, cct dbSNP:761573030
1084 1084 a, c dbSNP:781257967
1105 1105 a, c, t dbSNP:748005608
1106 1106 a, c, g dbSNP:772906477
1109 1109 a, c dbSNP:532030990
1117 1117 c, t dbSNP:137985872
1118 1118 a, g dbSNP:201352056
1123 1123 c, t dbSNP:370508486
1124 1124 a, g dbSNP:11570351
1125 1125 c, t dbSNP:761106361
1127 1127 c, g dbSNP:764620452
1129 1129 a, g dbSNP:754204521

Target ORF information:

RefSeq Version XM_006723734
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens endothelin 3 (EDN3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006723734

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16242
Accession Version NM_207034.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC008876.2. This sequence is a reference standard in the RefSeqGene project. On Oct 22, 2014 this sequence version replaced gi:46370063. Summary: The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]. Transcript Variant: This variant (4) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008876.2, J05081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT TGAAGTCAAG
GACCAACAAA GCAAGCAGGC TTTAGACCTC CACCATCCAA AGCTCATGCC CGGCAGTGGA
CTCGCCCTCG CTCCATCTAC CTGCCCCCGC TGCCTCTTTC AGGAAGGAGC CCCTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_996917.1
CDS387..1103
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..740(+)
Misc Feature(4)735..737(+)
Misc Feature(5)765..767(+)
Misc Feature(6)858..923(+)
Misc Feature(7)861..905(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..974
Gene:EDN3
Gene Synonym:
Exon (5)975..2636
Gene:EDN3
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:538954140
73 73 g, t dbSNP:149334
95 95 a, g dbSNP:553995768
122 122 c, g dbSNP:572327630
139 139 a, g dbSNP:542818479
174 174 c, t dbSNP:11570253
175 175 c, g dbSNP:555127949
194 194 g, t dbSNP:545445596
195 195 c, g dbSNP:573681306
202 202 g, t dbSNP:780505724
223 223 a, g dbSNP:749699689
224 224 c, t dbSNP:544294058
241 241 a, c dbSNP:559509661
264 264 a, g dbSNP:562510121
301 301 a, g dbSNP:755284881
310 310 a, t dbSNP:11570254
326 326 c, t dbSNP:545325392
329 329 c, t dbSNP:777204838
343 343 c, t dbSNP:368814466
346 346 a, c, g, t dbSNP:371551500
347 347 c, t dbSNP:781752092
353 353 a, c, t dbSNP:748531162
356 356 g, t dbSNP:527501488
364 364 a, g dbSNP:749491983
368 368 a, c dbSNP:375594972
369 369 a, g dbSNP:774507126
371 371 c, g dbSNP:759572315
372 372 c, g dbSNP:764022228
374 374 c, g dbSNP:776492495
381 381 a, g dbSNP:761651395
382 382 a, g dbSNP:149697
396 396 a, g dbSNP:567434377
397 397 c, g dbSNP:202140125
405 405 c, t dbSNP:758047604
410 410 g, t dbSNP:765898330
418 418 g, t dbSNP:751116353
421 421 c, t dbSNP:754475513
428 428 c, t dbSNP:781768805
429 429 g, t dbSNP:374697035
434 434 c, t dbSNP:550205592
435 435 a, g, t dbSNP:11570255
438 438 a, g dbSNP:457755
443 443 a, c, t dbSNP:532453805
447 447 c, t dbSNP:747041914
448 448 c, g dbSNP:769802593
466 466 a, c dbSNP:772960457
469 469 c, t dbSNP:762824805
477 477 a, c dbSNP:770621680
479 479 c, t dbSNP:774217144
480 480 a, g dbSNP:369335129
484 484 a, c, t dbSNP:371691396
488 488 a, c, t dbSNP:760033782
490 490 a, g dbSNP:754287022
493 493 c, t dbSNP:757704427
496 496 a, c dbSNP:779215514
503 503 a, c dbSNP:750694303
507 507 a, g, t dbSNP:758644212
508 508 g, t dbSNP:747096907
516 516 a, g dbSNP:761584084
521 521 c, t dbSNP:143959111
523 523 a, g dbSNP:777826972
527 527 a, g dbSNP:113842164
530 530 c, g dbSNP:749180844
532 532 c, t dbSNP:148646322
545 545 c, t dbSNP:770806114
549 549 a, g dbSNP:774307682
553 553 a, g dbSNP:369619472
565 565 c, g dbSNP:745615264
567 567 c, t dbSNP:373609215
573 573 a, g dbSNP:775223561
576 576 a, g dbSNP:760290378
577 577 a, g dbSNP:457651
578 578 g, t dbSNP:777233311
579 579 a, g dbSNP:762206123
586 586 -, tgg dbSNP:748397146
588 588 c, t dbSNP:765686063
589 589 -, t dbSNP:772230072
589 589 c, t dbSNP:750818786
591 591 a, g dbSNP:758697348
592 592 c, t dbSNP:766605338
594 594 a, g dbSNP:369429604
604 604 a, g dbSNP:376041123
608 608 a, c dbSNP:781103592
611 611 c, t dbSNP:565731779
620 620 c, t dbSNP:757170661
625 625 a, g dbSNP:778923787
630 630 a, g dbSNP:370091378
637 637 a, c, t dbSNP:142051051
640 640 c, g, t dbSNP:150731720
642 642 a, g dbSNP:776028645
643 643 a, t dbSNP:761348961
646 646 g, t dbSNP:765738929
647 647 a, g dbSNP:139978625
648 648 g, t dbSNP:763369291
651 651 c, t dbSNP:144937074
652 652 c, t dbSNP:751850788
654 654 c, g dbSNP:147917971
656 656 a, g dbSNP:767736650
663 663 c, g dbSNP:267606779
664 664 a, g dbSNP:752720292
669 669 a, g dbSNP:756201310
673 673 a, g dbSNP:141776410
679 679 a, c dbSNP:745795470
680 680 c, g dbSNP:145414258
687 687 a, g dbSNP:779690794
688 688 c, t dbSNP:746771631
692 692 c, g dbSNP:768226122
695 695 a, g dbSNP:780830178
709 709 c, t dbSNP:747625344
716 716 c, t dbSNP:769283989
718 718 a, g dbSNP:773779627
721 721 a, g dbSNP:267606778
722 722 c, g dbSNP:763421771
726 726 a, g dbSNP:771336824
731 731 c, t dbSNP:149729435
736 736 c, g dbSNP:774607474
742 742 -, ac dbSNP:776300941
742 742 a, t dbSNP:202126740
746 746 c, t dbSNP:759875525
747 747 c, g dbSNP:767643055
748 748 c, g, t dbSNP:267606028
758 758 a, g dbSNP:781011983
759 759 g, t dbSNP:34604265
766 766 a, g dbSNP:752400458
777 777 a, g dbSNP:755789488
779 779 c, g, t dbSNP:777336048
790 790 g, t dbSNP:374873073
796 796 a, g, t dbSNP:779352707
800 800 c, t dbSNP:772369425
811 811 c, t dbSNP:536097197
812 812 a, g dbSNP:187049336
829 829 a, g dbSNP:769005371
831 831 a, c dbSNP:776936096
837 837 c, t dbSNP:762043350
841 841 c, t dbSNP:765355566
844 844 a, g, t dbSNP:774197901
846 846 c, t dbSNP:767312223
847 847 a, g dbSNP:145239588
852 852 c, t dbSNP:755843195
856 856 c, t dbSNP:369295391
858 858 a, c dbSNP:753482233
860 860 c, t dbSNP:756755957
862 862 g, t dbSNP:74315384
863 863 c, t dbSNP:149177260
864 864 a, g dbSNP:778461734
866 866 c, t dbSNP:745314073
870 870 c, g dbSNP:758974269
872 872 a, g, t dbSNP:372385465
881 881 c, t dbSNP:575758752
887 887 a, g dbSNP:143232619
888 888 a, g dbSNP:748374290
893 893 a, c, t dbSNP:74315385
894 894 c, t dbSNP:151250216
896 896 a, g dbSNP:779957841
911 911 a, g dbSNP:34780366
920 920 c, t dbSNP:374020215
921 921 a, g dbSNP:767453373
928 928 g, t dbSNP:576523143
940 940 c, t dbSNP:368327225
941 941 a, g dbSNP:770991287
945 945 -, a dbSNP:11570344
951 951 -, a dbSNP:397843910
953 953 -, ag dbSNP:769685879
958 958 -, aag dbSNP:775484472
960 960 c, g dbSNP:775443105
971 971 c, g dbSNP:760582150
974 974 a, g dbSNP:768529772
976 976 g, t dbSNP:757499758
981 981 a, g dbSNP:779040805
985 985 a, t dbSNP:145582774
986 986 a, g dbSNP:745960429
989 989 c, t dbSNP:758459588
1013 1013 -, cct dbSNP:761573030
1016 1016 a, c dbSNP:781257967
1037 1037 a, c, t dbSNP:748005608
1038 1038 a, c, g dbSNP:772906477
1041 1041 a, c dbSNP:532030990
1049 1049 c, t dbSNP:137985872
1050 1050 a, g dbSNP:201352056
1055 1055 c, t dbSNP:370508486
1056 1056 a, g dbSNP:11570351
1057 1057 c, t dbSNP:761106361
1059 1059 c, g dbSNP:764620452
1061 1061 a, g dbSNP:754204521
1063 1063 c, t dbSNP:200741552
1071 1071 c, t dbSNP:757559436
1073 1073 c, t dbSNP:765500226
1074 1074 a, c, t dbSNP:372958987
1075 1075 a, c, g dbSNP:144250756
1083 1083 c, t dbSNP:549116987
1085 1085 c, t dbSNP:756038575
1088 1088 -, g dbSNP:767213063
1089 1089 a, g dbSNP:777702187
1093 1093 c, g dbSNP:749112686
1096 1096 c, t dbSNP:770841292
1097 1097 a, c dbSNP:774182574
1103 1103 a, g dbSNP:376137509
1104 1104 a, g, t dbSNP:138451394
1113 1113 c, t dbSNP:774891807
1117 1117 a, c dbSNP:760163643
1121 1121 a, g dbSNP:370288393
1129 1129 a, c dbSNP:777109038
1131 1131 c, t dbSNP:374488792
1132 1132 a, g dbSNP:368688196
1138 1138 -, c dbSNP:750823872
1139 1139 c, t dbSNP:750694380
1146 1146 a, t dbSNP:567494206
1147 1147 c, t dbSNP:200872063
1150 1150 c, g dbSNP:751597991
1161 1161 a, t dbSNP:463415
1168 1168 g, t dbSNP:555946641
1181 1181 g, t dbSNP:571072081
1184 1184 a, c dbSNP:538768879
1196 1196 a, t dbSNP:553876011
1243 1243 -, cc dbSNP:752653775
1243 1243 -, c dbSNP:11475273
1244 1244 -, c dbSNP:754876648
1245 1245 c, g dbSNP:372569173
1246 1246 a, c, g, t dbSNP:71321536
1248 1248 cc, t dbSNP:377130048
1249 1249 a, c, g, t dbSNP:11570352
1250 1250 a, c dbSNP:576673862
1251 1251 c, g dbSNP:544095029
1252 1252 a, c dbSNP:565501608
1252 1252 -, c dbSNP:397866060
1255 1255 c, t dbSNP:3026576
1268 1268 a, g dbSNP:11570353
1274 1274 a, g dbSNP:3026575
1280 1280 a, c dbSNP:11570354
1282 1282 c, g dbSNP:527379112
1308 1308 a, c dbSNP:548702985
1344 1344 a, g dbSNP:746504168
1421 1421 c, t dbSNP:567211087
1423 1423 g, t dbSNP:533368307
1458 1458 a, g dbSNP:79786751
1548 1548 c, t dbSNP:191496604
1553 1553 c, t dbSNP:546331828
1570 1570 c, g, t dbSNP:143382055
1578 1578 c, t dbSNP:538479580
1580 1580 a, g dbSNP:549881140
1634 1634 a, g dbSNP:11570355
1686 1686 c, t dbSNP:777130895
1698 1698 a, c dbSNP:566044970
1703 1703 a, g dbSNP:3026574
1703 1703 a, g dbSNP:11570356
1740 1740 a, g dbSNP:770384486
1757 1757 c, t dbSNP:778461322
1764 1764 c, g dbSNP:770340747
1811 1811 c, t dbSNP:746433345
1815 1815 a, t dbSNP:775994244
1819 1819 c, g dbSNP:531531144
1859 1859 c, t dbSNP:763311172
1860 1860 a, g dbSNP:57724975
1911 1911 a, c dbSNP:566560322
1955 1955 c, t dbSNP:775734185
1967 1967 c, g dbSNP:3026573
2004 2004 a, g dbSNP:764393299
2017 2017 c, t dbSNP:751714665
2018 2018 c, t dbSNP:11570357
2023 2023 g, t dbSNP:766229089
2025 2025 c, t dbSNP:559330652
2050 2050 c, g dbSNP:753749070
2060 2060 c, t dbSNP:3026572
2065 2065 c, t dbSNP:138371508
2066 2066 a, g dbSNP:559849084
2091 2091 c, t dbSNP:572054658
2109 2109 c, t dbSNP:542670565
2110 2110 c, g dbSNP:13037048
2115 2115 c, t dbSNP:11570358
2116 2116 a, g dbSNP:752457788
2193 2193 c, t dbSNP:181368086
2194 2194 a, g dbSNP:549350852
2226 2226 g, t dbSNP:376536945
2247 2247 c, t dbSNP:11570359
2250 2250 a, g dbSNP:3026571
2256 2256 -, tgtg dbSNP:569486179
2283 2283 c, t dbSNP:777354262
2299 2299 c, t dbSNP:547284575
2358 2358 c, t dbSNP:12322
2359 2359 a, g dbSNP:190002794
2384 2384 c, t dbSNP:746507182
2396 2396 c, t dbSNP:536369470
2399 2399 a, t dbSNP:770418007
2415 2415 a, g dbSNP:554865283
2447 2447 a, g dbSNP:780553745
2543 2543 a, g dbSNP:548692162
2586 2586 a, g dbSNP:183354781
2606 2606 a, c dbSNP:570404603
2608 2608 c, t dbSNP:746455039
2609 2609 c, t dbSNP:6026811
2621 2621 a, g dbSNP:6026812

Target ORF information:

RefSeq Version NM_207034
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207034

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT TGAAGTCAAG
GACCAACAAA GCAAGCAGGC TTTAGACCTC CACCATCCAA AGCTCATGCC CGGCAGTGGA
CTCGCCCTCG CTCCATCTAC CTGCCCCCGC TGCCTCTTTC AGGAAGGAGC CCCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16314
Accession Version NM_207033.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1, X52001.1 and BC008876.2. On Oct 22, 2014 this sequence version replaced gi:46370061. Summary: The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]. Transcript Variant: This variant (3) lacks an exon in the 3' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 4. The encoded isoform (3) is shorter and has two distinct internal amino acids, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52001.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_996916.1
CDS387..1061
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..737(+)
Misc Feature(4)765..767(+)
Misc Feature(5)858..923(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..2594
Gene:EDN3
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:538954140
73 73 g, t dbSNP:149334
95 95 a, g dbSNP:553995768
122 122 c, g dbSNP:572327630
139 139 a, g dbSNP:542818479
174 174 c, t dbSNP:11570253
175 175 c, g dbSNP:555127949
194 194 g, t dbSNP:545445596
195 195 c, g dbSNP:573681306
202 202 g, t dbSNP:780505724
223 223 a, g dbSNP:749699689
224 224 c, t dbSNP:544294058
241 241 a, c dbSNP:559509661
264 264 a, g dbSNP:562510121
301 301 a, g dbSNP:755284881
310 310 a, t dbSNP:11570254
326 326 c, t dbSNP:545325392
329 329 c, t dbSNP:777204838
343 343 c, t dbSNP:368814466
346 346 a, c, g, t dbSNP:371551500
347 347 c, t dbSNP:781752092
353 353 a, c, t dbSNP:748531162
356 356 g, t dbSNP:527501488
364 364 a, g dbSNP:749491983
368 368 a, c dbSNP:375594972
369 369 a, g dbSNP:774507126
371 371 c, g dbSNP:759572315
372 372 c, g dbSNP:764022228
374 374 c, g dbSNP:776492495
381 381 a, g dbSNP:761651395
382 382 a, g dbSNP:149697
396 396 a, g dbSNP:567434377
397 397 c, g dbSNP:202140125
405 405 c, t dbSNP:758047604
410 410 g, t dbSNP:765898330
418 418 g, t dbSNP:751116353
421 421 c, t dbSNP:754475513
428 428 c, t dbSNP:781768805
429 429 g, t dbSNP:374697035
434 434 c, t dbSNP:550205592
435 435 a, g, t dbSNP:11570255
438 438 a, g dbSNP:457755
443 443 a, c, t dbSNP:532453805
447 447 c, t dbSNP:747041914
448 448 c, g dbSNP:769802593
466 466 a, c dbSNP:772960457
469 469 c, t dbSNP:762824805
477 477 a, c dbSNP:770621680
479 479 c, t dbSNP:774217144
480 480 a, g dbSNP:369335129
484 484 a, c, t dbSNP:371691396
488 488 a, c, t dbSNP:760033782
490 490 a, g dbSNP:754287022
493 493 c, t dbSNP:757704427
496 496 a, c dbSNP:779215514
503 503 a, c dbSNP:750694303
507 507 a, g, t dbSNP:758644212
508 508 g, t dbSNP:747096907
516 516 a, g dbSNP:761584084
521 521 c, t dbSNP:143959111
523 523 a, g dbSNP:777826972
527 527 a, g dbSNP:113842164
530 530 c, g dbSNP:749180844
532 532 c, t dbSNP:148646322
545 545 c, t dbSNP:770806114
549 549 a, g dbSNP:774307682
553 553 a, g dbSNP:369619472
565 565 c, g dbSNP:745615264
567 567 c, t dbSNP:373609215
573 573 a, g dbSNP:775223561
576 576 a, g dbSNP:760290378
577 577 a, g dbSNP:457651
578 578 g, t dbSNP:777233311
579 579 a, g dbSNP:762206123
586 586 -, tgg dbSNP:748397146
588 588 c, t dbSNP:765686063
589 589 -, t dbSNP:772230072
589 589 c, t dbSNP:750818786
591 591 a, g dbSNP:758697348
592 592 c, t dbSNP:766605338
594 594 a, g dbSNP:369429604
604 604 a, g dbSNP:376041123
608 608 a, c dbSNP:781103592
611 611 c, t dbSNP:565731779
620 620 c, t dbSNP:757170661
625 625 a, g dbSNP:778923787
630 630 a, g dbSNP:370091378
637 637 a, c, t dbSNP:142051051
640 640 c, g, t dbSNP:150731720
642 642 a, g dbSNP:776028645
643 643 a, t dbSNP:761348961
646 646 g, t dbSNP:765738929
647 647 a, g dbSNP:139978625
648 648 g, t dbSNP:763369291
651 651 c, t dbSNP:144937074
652 652 c, t dbSNP:751850788
654 654 c, g dbSNP:147917971
656 656 a, g dbSNP:767736650
663 663 c, g dbSNP:267606779
664 664 a, g dbSNP:752720292
669 669 a, g dbSNP:756201310
673 673 a, g dbSNP:141776410
679 679 a, c dbSNP:745795470
680 680 c, g dbSNP:145414258
687 687 a, g dbSNP:779690794
688 688 c, t dbSNP:746771631
692 692 c, g dbSNP:768226122
695 695 a, g dbSNP:780830178
709 709 c, t dbSNP:747625344
716 716 c, t dbSNP:769283989
718 718 a, g dbSNP:773779627
721 721 a, g dbSNP:267606778
722 722 c, g dbSNP:763421771
726 726 a, g dbSNP:771336824
731 731 c, t dbSNP:149729435
736 736 c, g dbSNP:774607474
742 742 -, ac dbSNP:776300941
742 742 a, t dbSNP:202126740
746 746 c, t dbSNP:759875525
747 747 c, g dbSNP:767643055
748 748 c, g, t dbSNP:267606028
758 758 a, g dbSNP:781011983
759 759 g, t dbSNP:34604265
766 766 a, g dbSNP:752400458
777 777 a, g dbSNP:755789488
779 779 c, g, t dbSNP:777336048
790 790 g, t dbSNP:374873073
796 796 a, g, t dbSNP:779352707
800 800 c, t dbSNP:772369425
811 811 c, t dbSNP:536097197
812 812 a, g dbSNP:187049336
829 829 a, g dbSNP:769005371
831 831 a, c dbSNP:776936096
837 837 c, t dbSNP:762043350
841 841 c, t dbSNP:765355566
844 844 a, g, t dbSNP:774197901
846 846 c, t dbSNP:767312223
847 847 a, g dbSNP:145239588
852 852 c, t dbSNP:755843195
856 856 c, t dbSNP:369295391
858 858 a, c dbSNP:753482233
860 860 c, t dbSNP:756755957
862 862 g, t dbSNP:74315384
863 863 c, t dbSNP:149177260
864 864 a, g dbSNP:778461734
866 866 c, t dbSNP:745314073
870 870 c, g dbSNP:758974269
872 872 a, g, t dbSNP:372385465
881 881 c, t dbSNP:575758752
887 887 a, g dbSNP:143232619
888 888 a, g dbSNP:748374290
893 893 a, c, t dbSNP:74315385
894 894 c, t dbSNP:151250216
896 896 a, g dbSNP:779957841
911 911 a, g dbSNP:34780366
920 920 c, t dbSNP:374020215
921 921 a, g dbSNP:767453373
928 928 g, t dbSNP:576523143
929 929 a, g dbSNP:748824746
934 934 g, t dbSNP:757499758
939 939 a, g dbSNP:779040805
943 943 a, t dbSNP:145582774
944 944 a, g dbSNP:745960429
947 947 c, t dbSNP:758459588
971 971 -, cct dbSNP:761573030
974 974 a, c dbSNP:781257967
995 995 a, c, t dbSNP:748005608
996 996 a, c, g dbSNP:772906477
999 999 a, c dbSNP:532030990
1007 1007 c, t dbSNP:137985872
1008 1008 a, g dbSNP:201352056
1013 1013 c, t dbSNP:370508486
1014 1014 a, g dbSNP:11570351
1015 1015 c, t dbSNP:761106361
1017 1017 c, g dbSNP:764620452
1019 1019 a, g dbSNP:754204521
1021 1021 c, t dbSNP:200741552
1029 1029 c, t dbSNP:757559436
1031 1031 c, t dbSNP:765500226
1032 1032 a, c, t dbSNP:372958987
1033 1033 a, c, g dbSNP:144250756
1041 1041 c, t dbSNP:549116987
1043 1043 c, t dbSNP:756038575
1046 1046 -, g dbSNP:767213063
1047 1047 a, g dbSNP:777702187
1051 1051 c, g dbSNP:749112686
1054 1054 c, t dbSNP:770841292
1055 1055 a, c dbSNP:774182574
1061 1061 a, g dbSNP:376137509
1062 1062 a, g, t dbSNP:138451394
1071 1071 c, t dbSNP:774891807
1075 1075 a, c dbSNP:760163643
1079 1079 a, g dbSNP:370288393
1087 1087 a, c dbSNP:777109038
1089 1089 c, t dbSNP:374488792
1090 1090 a, g dbSNP:368688196
1096 1096 -, c dbSNP:750823872
1097 1097 c, t dbSNP:750694380
1104 1104 a, t dbSNP:567494206
1105 1105 c, t dbSNP:200872063
1108 1108 c, g dbSNP:751597991
1119 1119 a, t dbSNP:463415
1126 1126 g, t dbSNP:555946641
1139 1139 g, t dbSNP:571072081
1142 1142 a, c dbSNP:538768879
1154 1154 a, t dbSNP:553876011
1201 1201 -, cc dbSNP:752653775
1201 1201 -, c dbSNP:11475273
1202 1202 -, c dbSNP:754876648
1203 1203 c, g dbSNP:372569173
1204 1204 a, c, g, t dbSNP:71321536
1206 1206 cc, t dbSNP:377130048
1207 1207 a, c, g, t dbSNP:11570352
1208 1208 a, c dbSNP:576673862
1209 1209 c, g dbSNP:544095029
1210 1210 a, c dbSNP:565501608
1210 1210 -, c dbSNP:397866060
1213 1213 c, t dbSNP:3026576
1226 1226 a, g dbSNP:11570353
1232 1232 a, g dbSNP:3026575
1238 1238 a, c dbSNP:11570354
1240 1240 c, g dbSNP:527379112
1266 1266 a, c dbSNP:548702985
1302 1302 a, g dbSNP:746504168
1379 1379 c, t dbSNP:567211087
1381 1381 g, t dbSNP:533368307
1416 1416 a, g dbSNP:79786751
1506 1506 c, t dbSNP:191496604
1511 1511 c, t dbSNP:546331828
1528 1528 c, g, t dbSNP:143382055
1536 1536 c, t dbSNP:538479580
1538 1538 a, g dbSNP:549881140
1592 1592 a, g dbSNP:11570355
1644 1644 c, t dbSNP:777130895
1656 1656 a, c dbSNP:566044970
1661 1661 a, g dbSNP:3026574
1661 1661 a, g dbSNP:11570356
1698 1698 a, g dbSNP:770384486
1715 1715 c, t dbSNP:778461322
1722 1722 c, g dbSNP:770340747
1769 1769 c, t dbSNP:746433345
1773 1773 a, t dbSNP:775994244
1777 1777 c, g dbSNP:531531144
1817 1817 c, t dbSNP:763311172
1818 1818 a, g dbSNP:57724975
1869 1869 a, c dbSNP:566560322
1913 1913 c, t dbSNP:775734185
1925 1925 c, g dbSNP:3026573
1962 1962 a, g dbSNP:764393299
1975 1975 c, t dbSNP:751714665
1976 1976 c, t dbSNP:11570357
1981 1981 g, t dbSNP:766229089
1983 1983 c, t dbSNP:559330652
2008 2008 c, g dbSNP:753749070
2018 2018 c, t dbSNP:3026572
2023 2023 c, t dbSNP:138371508
2024 2024 a, g dbSNP:559849084
2049 2049 c, t dbSNP:572054658
2067 2067 c, t dbSNP:542670565
2068 2068 c, g dbSNP:13037048
2073 2073 c, t dbSNP:11570358
2074 2074 a, g dbSNP:752457788
2151 2151 c, t dbSNP:181368086
2152 2152 a, g dbSNP:549350852
2184 2184 g, t dbSNP:376536945
2205 2205 c, t dbSNP:11570359
2208 2208 a, g dbSNP:3026571
2214 2214 -, tgtg dbSNP:569486179
2241 2241 c, t dbSNP:777354262
2257 2257 c, t dbSNP:547284575
2316 2316 c, t dbSNP:12322
2317 2317 a, g dbSNP:190002794
2342 2342 c, t dbSNP:746507182
2354 2354 c, t dbSNP:536369470
2357 2357 a, t dbSNP:770418007
2373 2373 a, g dbSNP:554865283
2405 2405 a, g dbSNP:780553745
2501 2501 a, g dbSNP:548692162
2544 2544 a, g dbSNP:183354781
2564 2564 a, c dbSNP:570404603
2566 2566 c, t dbSNP:746455039
2567 2567 c, t dbSNP:6026811
2579 2579 a, g dbSNP:6026812

Target ORF information:

RefSeq Version NM_207033
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207033

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGACAGGTTG AAGTCAAGGA CCAACAAAGC AAGCAGGCTT TAGACCTCCA CCATCCAAAG
CTCATGCCCG GCAGTGGACT CGCCCTCGCT CCATCTACCT GCCCCCGCTG CCTCTTTCAG
GAAGGAGCCC CTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16162
Accession Version NM_207032.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1 and BC008876.2. On Oct 22, 2014 this sequence version replaced gi:46370059. Summary: The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 4. The encoded isoform (2), is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTTGAAG TCAAGGACCA ACAAAGCAAG CAGGCTTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_996915.1
CDS387..1046
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..740(+)
Misc Feature(4)735..737(+)
Misc Feature(5)765..767(+)
Misc Feature(6)858..923(+)
Misc Feature(7)861..905(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..1009
Gene:EDN3
Gene Synonym:
Exon (5)1010..2671
Gene:EDN3
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:538954140
73 73 g, t dbSNP:149334
95 95 a, g dbSNP:553995768
122 122 c, g dbSNP:572327630
139 139 a, g dbSNP:542818479
174 174 c, t dbSNP:11570253
175 175 c, g dbSNP:555127949
194 194 g, t dbSNP:545445596
195 195 c, g dbSNP:573681306
202 202 g, t dbSNP:780505724
223 223 a, g dbSNP:749699689
224 224 c, t dbSNP:544294058
241 241 a, c dbSNP:559509661
264 264 a, g dbSNP:562510121
301 301 a, g dbSNP:755284881
310 310 a, t dbSNP:11570254
326 326 c, t dbSNP:545325392
329 329 c, t dbSNP:777204838
343 343 c, t dbSNP:368814466
346 346 a, c, g, t dbSNP:371551500
347 347 c, t dbSNP:781752092
353 353 a, c, t dbSNP:748531162
356 356 g, t dbSNP:527501488
364 364 a, g dbSNP:749491983
368 368 a, c dbSNP:375594972
369 369 a, g dbSNP:774507126
371 371 c, g dbSNP:759572315
372 372 c, g dbSNP:764022228
374 374 c, g dbSNP:776492495
381 381 a, g dbSNP:761651395
382 382 a, g dbSNP:149697
396 396 a, g dbSNP:567434377
397 397 c, g dbSNP:202140125
405 405 c, t dbSNP:758047604
410 410 g, t dbSNP:765898330
418 418 g, t dbSNP:751116353
421 421 c, t dbSNP:754475513
428 428 c, t dbSNP:781768805
429 429 g, t dbSNP:374697035
434 434 c, t dbSNP:550205592
435 435 a, g, t dbSNP:11570255
438 438 a, g dbSNP:457755
443 443 a, c, t dbSNP:532453805
447 447 c, t dbSNP:747041914
448 448 c, g dbSNP:769802593
466 466 a, c dbSNP:772960457
469 469 c, t dbSNP:762824805
477 477 a, c dbSNP:770621680
479 479 c, t dbSNP:774217144
480 480 a, g dbSNP:369335129
484 484 a, c, t dbSNP:371691396
488 488 a, c, t dbSNP:760033782
490 490 a, g dbSNP:754287022
493 493 c, t dbSNP:757704427
496 496 a, c dbSNP:779215514
503 503 a, c dbSNP:750694303
507 507 a, g, t dbSNP:758644212
508 508 g, t dbSNP:747096907
516 516 a, g dbSNP:761584084
521 521 c, t dbSNP:143959111
523 523 a, g dbSNP:777826972
527 527 a, g dbSNP:113842164
530 530 c, g dbSNP:749180844
532 532 c, t dbSNP:148646322
545 545 c, t dbSNP:770806114
549 549 a, g dbSNP:774307682
553 553 a, g dbSNP:369619472
565 565 c, g dbSNP:745615264
567 567 c, t dbSNP:373609215
573 573 a, g dbSNP:775223561
576 576 a, g dbSNP:760290378
577 577 a, g dbSNP:457651
578 578 g, t dbSNP:777233311
579 579 a, g dbSNP:762206123
586 586 -, tgg dbSNP:748397146
588 588 c, t dbSNP:765686063
589 589 -, t dbSNP:772230072
589 589 c, t dbSNP:750818786
591 591 a, g dbSNP:758697348
592 592 c, t dbSNP:766605338
594 594 a, g dbSNP:369429604
604 604 a, g dbSNP:376041123
608 608 a, c dbSNP:781103592
611 611 c, t dbSNP:565731779
620 620 c, t dbSNP:757170661
625 625 a, g dbSNP:778923787
630 630 a, g dbSNP:370091378
637 637 a, c, t dbSNP:142051051
640 640 c, g, t dbSNP:150731720
642 642 a, g dbSNP:776028645
643 643 a, t dbSNP:761348961
646 646 g, t dbSNP:765738929
647 647 a, g dbSNP:139978625
648 648 g, t dbSNP:763369291
651 651 c, t dbSNP:144937074
652 652 c, t dbSNP:751850788
654 654 c, g dbSNP:147917971
656 656 a, g dbSNP:767736650
663 663 c, g dbSNP:267606779
664 664 a, g dbSNP:752720292
669 669 a, g dbSNP:756201310
673 673 a, g dbSNP:141776410
679 679 a, c dbSNP:745795470
680 680 c, g dbSNP:145414258
687 687 a, g dbSNP:779690794
688 688 c, t dbSNP:746771631
692 692 c, g dbSNP:768226122
695 695 a, g dbSNP:780830178
709 709 c, t dbSNP:747625344
716 716 c, t dbSNP:769283989
718 718 a, g dbSNP:773779627
721 721 a, g dbSNP:267606778
722 722 c, g dbSNP:763421771
726 726 a, g dbSNP:771336824
731 731 c, t dbSNP:149729435
736 736 c, g dbSNP:774607474
742 742 -, ac dbSNP:776300941
742 742 a, t dbSNP:202126740
746 746 c, t dbSNP:759875525
747 747 c, g dbSNP:767643055
748 748 c, g, t dbSNP:267606028
758 758 a, g dbSNP:781011983
759 759 g, t dbSNP:34604265
766 766 a, g dbSNP:752400458
777 777 a, g dbSNP:755789488
779 779 c, g, t dbSNP:777336048
790 790 g, t dbSNP:374873073
796 796 a, g, t dbSNP:779352707
800 800 c, t dbSNP:772369425
811 811 c, t dbSNP:536097197
812 812 a, g dbSNP:187049336
829 829 a, g dbSNP:769005371
831 831 a, c dbSNP:776936096
837 837 c, t dbSNP:762043350
841 841 c, t dbSNP:765355566
844 844 a, g, t dbSNP:774197901
846 846 c, t dbSNP:767312223
847 847 a, g dbSNP:145239588
852 852 c, t dbSNP:755843195
856 856 c, t dbSNP:369295391
858 858 a, c dbSNP:753482233
860 860 c, t dbSNP:756755957
862 862 g, t dbSNP:74315384
863 863 c, t dbSNP:149177260
864 864 a, g dbSNP:778461734
866 866 c, t dbSNP:745314073
870 870 c, g dbSNP:758974269
872 872 a, g, t dbSNP:372385465
881 881 c, t dbSNP:575758752
887 887 a, g dbSNP:143232619
888 888 a, g dbSNP:748374290
893 893 a, c, t dbSNP:74315385
894 894 c, t dbSNP:151250216
896 896 a, g dbSNP:779957841
911 911 a, g dbSNP:34780366
920 920 c, t dbSNP:374020215
921 921 a, g dbSNP:767453373
928 928 g, t dbSNP:576523143
940 940 c, t dbSNP:368327225
941 941 a, g dbSNP:770991287
945 945 -, a dbSNP:11570344
951 951 -, a dbSNP:397843910
953 953 -, ag dbSNP:769685879
958 958 -, aag dbSNP:775484472
960 960 c, g dbSNP:775443105
971 971 c, g dbSNP:760582150
974 974 a, g dbSNP:768529772
993 993 a, g dbSNP:776520707
996 996 c, g, t dbSNP:761447718
1011 1011 g, t dbSNP:757499758
1016 1016 a, g dbSNP:779040805
1020 1020 a, t dbSNP:145582774
1021 1021 a, g dbSNP:745960429
1024 1024 c, t dbSNP:758459588
1048 1048 -, cct dbSNP:761573030
1051 1051 a, c dbSNP:781257967
1072 1072 a, c, t dbSNP:748005608
1073 1073 a, c, g dbSNP:772906477
1076 1076 a, c dbSNP:532030990
1084 1084 c, t dbSNP:137985872
1085 1085 a, g dbSNP:201352056
1090 1090 c, t dbSNP:370508486
1091 1091 a, g dbSNP:11570351
1092 1092 c, t dbSNP:761106361
1094 1094 c, g dbSNP:764620452
1096 1096 a, g dbSNP:754204521
1098 1098 c, t dbSNP:200741552
1106 1106 c, t dbSNP:757559436
1108 1108 c, t dbSNP:765500226
1109 1109 a, c, t dbSNP:372958987
1110 1110 a, c, g dbSNP:144250756
1118 1118 c, t dbSNP:549116987
1120 1120 c, t dbSNP:756038575
1123 1123 -, g dbSNP:767213063
1124 1124 a, g dbSNP:777702187
1128 1128 c, g dbSNP:749112686
1131 1131 c, t dbSNP:770841292
1132 1132 a, c dbSNP:774182574
1138 1138 a, g dbSNP:376137509
1139 1139 a, g, t dbSNP:138451394
1148 1148 c, t dbSNP:774891807
1152 1152 a, c dbSNP:760163643
1156 1156 a, g dbSNP:370288393
1164 1164 a, c dbSNP:777109038
1166 1166 c, t dbSNP:374488792
1167 1167 a, g dbSNP:368688196
1173 1173 -, c dbSNP:750823872
1174 1174 c, t dbSNP:750694380
1181 1181 a, t dbSNP:567494206
1182 1182 c, t dbSNP:200872063
1185 1185 c, g dbSNP:751597991
1196 1196 a, t dbSNP:463415
1203 1203 g, t dbSNP:555946641
1216 1216 g, t dbSNP:571072081
1219 1219 a, c dbSNP:538768879
1231 1231 a, t dbSNP:553876011
1278 1278 -, cc dbSNP:752653775
1278 1278 -, c dbSNP:11475273
1279 1279 -, c dbSNP:754876648
1280 1280 c, g dbSNP:372569173
1281 1281 a, c, g, t dbSNP:71321536
1283 1283 cc, t dbSNP:377130048
1284 1284 a, c, g, t dbSNP:11570352
1285 1285 a, c dbSNP:576673862
1286 1286 c, g dbSNP:544095029
1287 1287 a, c dbSNP:565501608
1287 1287 -, c dbSNP:397866060
1290 1290 c, t dbSNP:3026576
1303 1303 a, g dbSNP:11570353
1309 1309 a, g dbSNP:3026575
1315 1315 a, c dbSNP:11570354
1317 1317 c, g dbSNP:527379112
1343 1343 a, c dbSNP:548702985
1379 1379 a, g dbSNP:746504168
1456 1456 c, t dbSNP:567211087
1458 1458 g, t dbSNP:533368307
1493 1493 a, g dbSNP:79786751
1583 1583 c, t dbSNP:191496604
1588 1588 c, t dbSNP:546331828
1605 1605 c, g, t dbSNP:143382055
1613 1613 c, t dbSNP:538479580
1615 1615 a, g dbSNP:549881140
1669 1669 a, g dbSNP:11570355
1721 1721 c, t dbSNP:777130895
1733 1733 a, c dbSNP:566044970
1738 1738 a, g dbSNP:3026574
1738 1738 a, g dbSNP:11570356
1775 1775 a, g dbSNP:770384486
1792 1792 c, t dbSNP:778461322
1799 1799 c, g dbSNP:770340747
1846 1846 c, t dbSNP:746433345
1850 1850 a, t dbSNP:775994244
1854 1854 c, g dbSNP:531531144
1894 1894 c, t dbSNP:763311172
1895 1895 a, g dbSNP:57724975
1946 1946 a, c dbSNP:566560322
1990 1990 c, t dbSNP:775734185
2002 2002 c, g dbSNP:3026573
2039 2039 a, g dbSNP:764393299
2052 2052 c, t dbSNP:751714665
2053 2053 c, t dbSNP:11570357
2058 2058 g, t dbSNP:766229089
2060 2060 c, t dbSNP:559330652
2085 2085 c, g dbSNP:753749070
2095 2095 c, t dbSNP:3026572
2100 2100 c, t dbSNP:138371508
2101 2101 a, g dbSNP:559849084
2126 2126 c, t dbSNP:572054658
2144 2144 c, t dbSNP:542670565
2145 2145 c, g dbSNP:13037048
2150 2150 c, t dbSNP:11570358
2151 2151 a, g dbSNP:752457788
2228 2228 c, t dbSNP:181368086
2229 2229 a, g dbSNP:549350852
2261 2261 g, t dbSNP:376536945
2282 2282 c, t dbSNP:11570359
2285 2285 a, g dbSNP:3026571
2291 2291 -, tgtg dbSNP:569486179
2318 2318 c, t dbSNP:777354262
2334 2334 c, t dbSNP:547284575
2393 2393 c, t dbSNP:12322
2394 2394 a, g dbSNP:190002794
2419 2419 c, t dbSNP:746507182
2431 2431 c, t dbSNP:536369470
2434 2434 a, t dbSNP:770418007
2450 2450 a, g dbSNP:554865283
2482 2482 a, g dbSNP:780553745
2578 2578 a, g dbSNP:548692162
2621 2621 a, g dbSNP:183354781
2641 2641 a, c dbSNP:570404603
2643 2643 c, t dbSNP:746455039
2644 2644 c, t dbSNP:6026811
2656 2656 a, g dbSNP:6026812

Target ORF information:

RefSeq Version NM_207032
Organism Homo sapiens (human)
Definition Homo sapiens endothelin 3 (EDN3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207032

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT GAGAGGTGCC
AACAGAGGCC TGTGTCAAAG GAGGTTGAAG TCAAGGACCA ACAAAGCAAG CAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57427
Accession Version NM_001302455.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product endothelin-3 isoform 4 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF305608.1, BC053866.1, DN989853.1 and BC008876.2. Summary: The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]. Transcript Variant: This variant (5) uses an alternate splice site in the 3' terminal exon, which results in a frameshift and an early stop codon, compared to variant 4. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN989853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG 
CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGAC AGGTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001289384.1
CDS387..983
Misc Feature(1)375..377(+)
Misc Feature(2)663..752(+)
Misc Feature(3)735..740(+)
Misc Feature(4)858..923(+)
Misc Feature(5)861..905(+)
Exon (1)1..438
Gene:EDN3
Gene Synonym:
Exon (2)439..751
Gene:EDN3
Gene Synonym:
Exon (3)752..928
Gene:EDN3
Gene Synonym:
Exon (4)929..974
Gene:EDN3
Gene Synonym:
Exon (5)975..2640
Gene:EDN3
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, g dbSNP:538954140
73 73 g, t dbSNP:149334
95 95 a, g dbSNP:553995768
122 122 c, g dbSNP:572327630
139 139 a, g dbSNP:542818479
174 174 c, t dbSNP:11570253
175 175 c, g dbSNP:555127949
194 194 g, t dbSNP:545445596
195 195 c, g dbSNP:573681306
202