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EFNB1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EFNB1
Entrez Gene ID 1947
Full Name ephrin-B1
Synonyms CFND, CFNS, EFB1, EFL3, EPLG2, Elk-L, LERK2
General protein information
Preferred Names
ephrin-B1
Names
ephrin-B1
EFL-3
LERK-2
ELK ligand
ligand of eph-related kinase 2
eph-related receptor tyrosine kinase ligand 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq12

Summary The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300035

Disorder Html: Craniofrontonasal dysplasia, 304110 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004429 NP_004420 ephrin-B1 precursor



Homo sapiens (human) EFNB1 NP_004420.1
Pan troglodytes (chimpanzee) EFNB1 XP_529024.3
Macaca mulatta (Rhesus monkey) EFNB1 XP_001082851.2
Canis lupus familiaris (dog) EFNB1 XP_849150.2
Bos taurus (cattle) EFNB1 NP_001073768.1
Mus musculus (house mouse) Efnb1 NP_034240.1
Rattus norvegicus (Norway rat) Efnb1 NP_058785.2
Gallus gallus (chicken) EFNB1 NP_990366.1
Danio rerio (zebrafish) efnb1 NP_571880.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100494328 XP_002934941.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EFNB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFNB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26381
NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26381
Clone ID Related Accession (Same CDS sequence) NM_004429
Accession Version NM_004429.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1041bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product ephrin-B1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC016649.1, CB132233.1 and BM972960.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2008 this sequence version replaced gi:31317225. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016649.1, BC052979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 23335590 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCGGC CTGGGCAGCG TTGGCTCGGC AAGTGGCTTG TGGCGATGGT CGTGTGGGCG 
CTGTGCCGGC TCGCCACACC GCTGGCCAAG AACCTGGAGC CCGTATCCTG GAGCTCCCTC
AACCCCAAGT TCCTGAGTGG GAAGGGCTTG GTGATCTATC CGAAAATTGG AGACAAGCTG
GACATCATCT GCCCCCGAGC AGAAGCAGGG CGGCCCTATG AGTACTACAA GCTGTACCTG
GTGCGGCCTG AGCAGGCAGC TGCCTGTAGC ACAGTTCTCG ACCCCAACGT GTTGGTCACC
TGCAATAGGC CAGAGCAGGA AATACGCTTT ACCATCAAGT TCCAGGAGTT CAGCCCCAAC
TACATGGGCC TGGAGTTCAA GAAGCACCAT GATTACTACA TTACCTCAAC ATCCAATGGA
AGCCTGGAGG GGCTGGAAAA CCGGGAGGGC GGTGTGTGCC GCACACGCAC CATGAAGATC
ATCATGAAGG TTGGGCAAGA TCCCAATGCT GTGACGCCTG AGCAGCTGAC TACCAGCAGG
CCCAGCAAGG AGGCAGACAA CACTGTCAAG ATGGCCACAC AGGCCCCTGG TAGTCGGGGC
TCCCTGGGTG ACTCTGATGG CAAGCATGAG ACTGTGAACC AGGAAGAGAA GAGTGGCCCA
GGTGCAAGTG GGGGCAGCAG CGGGGACCCT GATGGCTTCT TCAACTCCAA GGTGGCATTG
TTCGCGGCTG TCGGTGCCGG TTGCGTCATC TTCCTGCTCA TCATCATCTT CCTGACGGTC
CTACTACTGA AGCTACGCAA GCGGCACCGC AAGCACACAC AGCAGCGGGC GGCTGCCCTC
TCGCTCAGTA CCCTGGCCAG TCCCAAGGGG GGCAGTGGCA CAGCGGGCAC CGAGCCCAGC
GACATCATCA TTCCCTTACG GACTACAGAG AACAACTACT GCCCCCACTA TGAGAAGGTG
AGTGGGGACT ACGGGCACCC TGTCTACATC GTCCAAGAGA TGCCGCCCCA GAGCCCGGCG
AACATCTACT ACAAGGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004420.1
CDS781..1821
Misc Feature(1)250..252(+)
Misc Feature(2)677..688(+)
Misc Feature(3)871..1275(+)
Misc Feature(4)955..1155(+)
Misc Feature(5)1492..1554(+)
Misc Feature(6)1525..>1761(+)
Misc Feature(7)1639..1641(+)
Misc Feature(8)1750..1752(+)
Misc Feature(9)1765..1767(+)
Misc Feature(10)1807..1809(+)
Misc Feature(11)1810..1818(+)
Exon (1)1..908
Gene:EFNB1
Gene Synonym:
Exon (2)909..1186
Gene:EFNB1
Gene Synonym:
Exon (3)1187..1279
Gene:EFNB1
Gene Synonym:
Exon (4)1280..1408
Gene:EFNB1
Gene Synonym:
Exon (5)1409..3331
Gene:EFNB1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004429
Organism Homo sapiens (human)
Definition Homo sapiens ephrin-B1 (EFNB1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004429

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTCGGC CTGGGCAGCG TTGGCTCGGC AAGTGGCTTG TGGCGATGGT CGTGTGGGCG 
CTGTGCCGGC TCGCCACACC GCTGGCCAAG AACCTGGAGC CCGTATCCTG GAGCTCCCTC
AACCCCAAGT TCCTGAGTGG GAAGGGCTTG GTGATCTATC CGAAAATTGG AGACAAGCTG
GACATCATCT GCCCCCGAGC AGAAGCAGGG CGGCCCTATG AGTACTACAA GCTGTACCTG
GTGCGGCCTG AGCAGGCAGC TGCCTGTAGC ACAGTTCTCG ACCCCAACGT GTTGGTCACC
TGCAATAGGC CAGAGCAGGA AATACGCTTT ACCATCAAGT TCCAGGAGTT CAGCCCCAAC
TACATGGGCC TGGAGTTCAA GAAGCACCAT GATTACTACA TTACCTCAAC ATCCAATGGA
AGCCTGGAGG GGCTGGAAAA CCGGGAGGGC GGTGTGTGCC GCACACGCAC CATGAAGATC
ATCATGAAGG TTGGGCAAGA TCCCAATGCT GTGACGCCTG AGCAGCTGAC TACCAGCAGG
CCCAGCAAGG AGGCAGACAA CACTGTCAAG ATGGCCACAC AGGCCCCTGG TAGTCGGGGC
TCCCTGGGTG ACTCTGATGG CAAGCATGAG ACTGTGAACC AGGAAGAGAA GAGTGGCCCA
GGTGCAAGTG GGGGCAGCAG CGGGGACCCT GATGGCTTCT TCAACTCCAA GGTGGCATTG
TTCGCGGCTG TCGGTGCCGG TTGCGTCATC TTCCTGCTCA TCATCATCTT CCTGACGGTC
CTACTACTGA AGCTACGCAA GCGGCACCGC AAGCACACAC AGCAGCGGGC GGCTGCCCTC
TCGCTCAGTA CCCTGGCCAG TCCCAAGGGG GGCAGTGGCA CAGCGGGCAC CGAGCCCAGC
GACATCATCA TTCCCTTACG GACTACAGAG AACAACTACT GCCCCCACTA TGAGAAGGTG
AGTGGGGACT ACGGGCACCC TGTCTACATC GTCCAAGAGA TGCCGCCCCA GAGCCCGGCG
AACATCTACT ACAAGGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Phenotypes of craniofrontonasal syndrome in patients with a pathogenic mutation in EFNB1
Eur. J. Hum. Genet. 22 (8), 995-1001 (2014)
van den Elzen ME, Twigg SR, Goos JA, Hoogeboom AJ, van den Ouweland AM, Wilkie AO and Mathijssen IM.


book

EphrinB1 interacts with CNK1 and promotes cell migration through c-Jun N-terminal kinase (JNK) activation
J. Biol. Chem. 289 (26), 18556-18568 (2014)
Cho HJ, Hwang YS, Mood K, Ji YJ, Lim J, Morrison DK and Daar IO.


book

Targeting ERBB receptors shifts their partners and triggers persistent ERK signaling through a novel ERBB/EFNB1 complex
Cancer Res. 73 (18), 5787-5797 (2013)
Vermeer PD, Colbert PL, Wieking BG, Vermeer DW and Lee JH.


book

Cellular interference in craniofrontonasal syndrome: males mosaic for mutations in the X-linked EFNB1 gene are more severely affected than true hemizygotes
Hum. Mol. Genet. 22 (8), 1654-1662 (2013)
Twigg SR, Babbs C, van den Elzen ME, Goriely A, Taylor S, McGowan SJ, Giannoulatou E, Lonie L, Ragoussis J, Sadighi Akha E, Knight SJ, Zechi-Ceide RM, Hoogeboom JA, Pober BR, Toriello HV, Wall SA, Rita Passos-Bueno M, Brunner HG, Mathijssen IM and Wilkie AO.


book

Enhanced expression of EphrinB1 is associated with lymph node metastasis and poor prognosis in breast cancer
Cancer Biomark 13 (4), 261-267 (2013)
Yin H, Lu C, Tang Y, Wang H, Wang H and Wang J.


book

The genes encoding the eph-related receptor tyrosine kinase ligands LERK-1 (EPLG1, Epl1), LERK-3 (EPLG3, Epl3), and LERK-4 (EPLG4, Epl4) are clustered on human chromosome 1 and mouse chromosome 3
Genomics 33 (2), 277-282 (1996)
Cerretti DP, Lyman SD, Kozlosky CJ, Copeland NG, Gilbert DJ, Jenkins NA, Valentine V, Kirstein MN, Shapiro DN and Morris SW.


book

Assignment of the gene (EPLG2) encoding a high-affinity binding protein for the receptor tyrosine kinase elk to a 200-kilobasepair region in human chromosome Xq12
Genomics 25 (1), 334-335 (1995)
Fletcher FA, Huebner K, Shaffer LG, Fairweather ND, Monaco AP, Muller U, Druck T, Simoneaux DK, Chelly J, Belmont JW et al.


book

Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity
Science 266 (5186), 816-819 (1994)
Davis S, Gale NW, Aldrich TH, Maisonpierre PC, Lhotak V, Pawson T, Goldfarb M and Yancopoulos GD.


book

Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors
EMBO J. 13 (16), 3757-3762 (1994)
Beckmann MP, Cerretti DP, Baum P, Vanden Bos T, James L, Farrah T, Kozlosky C, Hollingsworth T, Shilling H, Maraskovsky E et al.


book

Congenital Diaphragmatic Hernia Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Pober,B.R., Russell,M.K. and Ackerman,K.G.


 
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