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EGFR cDNA ORF clone, Homo sapiens (human)

Gene Symbol EGFR
Entrez Gene ID 1956
Full Name epidermal growth factor receptor
Synonyms ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7p12

Summary The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]. lac of sum
Disorder MIM:

131550

Disorder Html: Nonsmall cell lung cancer, response to tyrosine kinase inhibitor in,

mRNA and Protein(s)

mRNA Protein Name
NM_201282 NP_958439 epidermal growth factor receptor isoform b precursor
NM_201283 NP_958440 epidermal growth factor receptor isoform c precursor
NM_201284 NP_958441 epidermal growth factor receptor isoform d precursor
NM_005228 NP_005219 epidermal growth factor receptor isoform a precursor



Homo sapiens (human) EGFR NP_005219.2
Pan troglodytes (chimpanzee) EGFR XP_519102.3
Macaca mulatta (Rhesus monkey) EGFR XP_001107305.1
Canis lupus familiaris (dog) EGFR XP_533073.3
Bos taurus (cattle) EGFR XP_002696936.2
Mus musculus (house mouse) Egfr NP_997538.1
Rattus norvegicus (Norway rat) Egfr NP_113695.1
Gallus gallus (chicken) EGFR NP_990828.2
Danio rerio (zebrafish) egfra NP_919405.1
Xenopus (Silurana) tropicalis (western clawed frog) egfr XP_002939960.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EGFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25365
NM_201282 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25409
NM_201283 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$169.50-$237.30
$339.00
OHu25398 NM_201284 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $496.30
$709.00
OHu25437
NM_005228 Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$609.50-$853.30
$1219.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25365
Clone ID Related Accession (Same CDS sequence) NM_201282
Accession Version NM_201282.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1887bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product epidermal growth factor receptor isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1, AF125253.1 and AF277897.1. Transcript Variant: This variant (2) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_958439.1
CDS247..2133
Misc Feature(1)208..210(+)
Misc Feature(2)337..420(+)
Misc Feature(3)412..414(+)
Misc Feature(4)415..750(+)
Misc Feature(5)469..1146(+)
Misc Feature(6)628..630(+)
Misc Feature(7)715..807(+)
Misc Feature(8)769..771(+)
Misc Feature(9)799..1257(+)
Misc Feature(10)814..843(+)
Misc Feature(11)826..867(+)
Misc Feature(12)832..834(+)
Misc Feature(13)889..915(+)
Misc Feature(14)901..939(+)
Misc Feature(15)931..933(+)
Misc Feature(16)937..1068(+)
Misc Feature(17)940..966(+)
Misc Feature(18)952..990(+)
Misc Feature(19)997..1026(+)
Misc Feature(20)1036..1119(+)
Misc Feature(21)1129..1167(+)
Misc Feature(22)1177..1224(+)
Misc Feature(23)1231..1245(+)
Misc Feature(24)1255..1332(+)
Misc Feature(25)1300..1302(+)
Misc Feature(26)1327..1689(+)
Misc Feature(27)1327..1329(+)
Misc Feature(28)1414..2046(+)
Misc Feature(29)1483..1485(+)
Misc Feature(30)1576..1578(+)
Misc Feature(31)1654..1743(+)
Misc Feature(32)1759..2127(+)
Misc Feature(33)1762..1923(+)
Misc Feature(34)1762..1791(+)
Misc Feature(35)1774..1815(+)
Misc Feature(36)1822..1851(+)
Misc Feature(37)1828..1830(+)
Misc Feature(38)1861..1911(+)
Misc Feature(39)1918..>2040(+)
Misc Feature(40)1918..1959(+)
Misc Feature(41)1930..1983(+)
Misc Feature(42)1948..1950(+)
Misc Feature(43)1990..2019(+)
Misc Feature(44)2029..2097(+)
Misc Feature(45)2053..2055(+)
Misc Feature(46)2113..2115(+)
Exon (1)1..334
Gene:EGFR
Gene Synonym:
Exon (2)335..486
Gene:EGFR
Gene Synonym:
Exon (3)487..670
Gene:EGFR
Gene Synonym:
Exon (4)671..805
Gene:EGFR
Gene Synonym:
Exon (5)806..874
Gene:EGFR
Gene Synonym:
Exon (6)875..993
Gene:EGFR
Gene Synonym:
Exon (7)994..1135
Gene:EGFR
Gene Synonym:
Exon (8)1136..1252
Gene:EGFR
Gene Synonym:
Exon (9)1253..1379
Gene:EGFR
Gene Synonym:
Exon (10)1380..1453
Gene:EGFR
Gene Synonym:
Exon (11)1454..1544
Gene:EGFR
Gene Synonym:
Exon (12)1545..1744
Gene:EGFR
Gene Synonym:
Exon (13)1745..1877
Gene:EGFR
Gene Synonym:
Exon (14)1878..1968
Gene:EGFR
Gene Synonym:
Exon (15)1969..2126
Gene:EGFR
Gene Synonym:
Exon (16)2127..2239
Gene:EGFR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_201282
Organism Homo sapiens (human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201282

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25409
Clone ID Related Accession (Same CDS sequence) NM_201283
Accession Version NM_201283.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1218bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product epidermal growth factor receptor isoform c precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1 and U48722.1. Transcript Variant: This variant (3) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48722.1, AY698024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGTT TGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_958440.1
CDS247..1464
Misc Feature(1)208..210(+)
Misc Feature(2)337..420(+)
Misc Feature(3)412..414(+)
Misc Feature(4)415..750(+)
Misc Feature(5)469..1146(+)
Misc Feature(6)628..630(+)
Misc Feature(7)715..807(+)
Misc Feature(8)769..771(+)
Misc Feature(9)799..1257(+)
Misc Feature(10)814..843(+)
Misc Feature(11)826..867(+)
Misc Feature(12)832..834(+)
Misc Feature(13)889..915(+)
Misc Feature(14)901..939(+)
Misc Feature(15)931..933(+)
Misc Feature(16)937..1068(+)
Misc Feature(17)940..966(+)
Misc Feature(18)952..990(+)
Misc Feature(19)997..1026(+)
Misc Feature(20)1036..1119(+)
Misc Feature(21)1129..1167(+)
Misc Feature(22)1177..1224(+)
Misc Feature(23)1231..1245(+)
Misc Feature(24)1255..1332(+)
Misc Feature(25)1300..1302(+)
Misc Feature(26)1327..>1455(+)
Misc Feature(27)1327..1329(+)
Exon (1)1..334
Gene:EGFR
Gene Synonym:
Exon (2)335..486
Gene:EGFR
Gene Synonym:
Exon (3)487..670
Gene:EGFR
Gene Synonym:
Exon (4)671..805
Gene:EGFR
Gene Synonym:
Exon (5)806..874
Gene:EGFR
Gene Synonym:
Exon (6)875..993
Gene:EGFR
Gene Synonym:
Exon (7)994..1135
Gene:EGFR
Gene Synonym:
Exon (8)1136..1252
Gene:EGFR
Gene Synonym:
Exon (9)1253..1379
Gene:EGFR
Gene Synonym:
Exon (10)1380..1572
Gene:EGFR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_201283
Organism Homo sapiens (human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201283

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGTT TGAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25398
Clone ID Related Accession (Same CDS sequence) NM_201284
Accession Version NM_201284.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product epidermal growth factor receptor isoform d precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1 and AF125253.1. Transcript Variant: This variant (4) uses a different 3' terminal exon when compared to variant 1. The resulting isoform (d) has a shorter and distinct C-terminus. Only the extracellular domain is present in isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
GCCTCCTCCT GCCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_958441.1
CDS247..2364
Misc Feature(1)208..210(+)
Misc Feature(2)337..420(+)
Misc Feature(3)412..414(+)
Misc Feature(4)415..750(+)
Misc Feature(5)469..1146(+)
Misc Feature(6)628..630(+)
Misc Feature(7)715..807(+)
Misc Feature(8)769..771(+)
Misc Feature(9)799..1257(+)
Misc Feature(10)814..843(+)
Misc Feature(11)826..867(+)
Misc Feature(12)832..834(+)
Misc Feature(13)889..915(+)
Misc Feature(14)901..939(+)
Misc Feature(15)931..933(+)
Misc Feature(16)937..1068(+)
Misc Feature(17)940..966(+)
Misc Feature(18)952..990(+)
Misc Feature(19)997..1026(+)
Misc Feature(20)1036..1119(+)
Misc Feature(21)1129..1167(+)
Misc Feature(22)1177..1224(+)
Misc Feature(23)1231..1245(+)
Misc Feature(24)1255..1332(+)
Misc Feature(25)1300..1302(+)
Misc Feature(26)1327..1689(+)
Misc Feature(27)1327..1329(+)
Misc Feature(28)1414..2046(+)
Misc Feature(29)1483..1485(+)
Misc Feature(30)1576..1578(+)
Misc Feature(31)1654..1743(+)
Misc Feature(32)1759..2127(+)
Misc Feature(33)1762..1923(+)
Misc Feature(34)1762..1791(+)
Misc Feature(35)1774..1815(+)
Misc Feature(36)1822..1851(+)
Misc Feature(37)1828..1830(+)
Misc Feature(38)1861..1911(+)
Misc Feature(39)1918..>2040(+)
Misc Feature(40)1918..1959(+)
Misc Feature(41)1930..1983(+)
Misc Feature(42)1948..1950(+)
Misc Feature(43)1990..2019(+)
Misc Feature(44)2029..2097(+)
Misc Feature(45)2053..2055(+)
Misc Feature(46)2113..2115(+)
Misc Feature(47)2323..2325(+)
Exon (1)1..334
Gene:EGFR
Gene Synonym:
Exon (2)335..486
Gene:EGFR
Gene Synonym:
Exon (3)487..670
Gene:EGFR
Gene Synonym:
Exon (4)671..805
Gene:EGFR
Gene Synonym:
Exon (5)806..874
Gene:EGFR
Gene Synonym:
Exon (6)875..993
Gene:EGFR
Gene Synonym:
Exon (7)994..1135
Gene:EGFR
Gene Synonym:
Exon (8)1136..1252
Gene:EGFR
Gene Synonym:
Exon (9)1253..1379
Gene:EGFR
Gene Synonym:
Exon (10)1380..1453
Gene:EGFR
Gene Synonym:
Exon (11)1454..1544
Gene:EGFR
Gene Synonym:
Exon (12)1545..1744
Gene:EGFR
Gene Synonym:
Exon (13)1745..1877
Gene:EGFR
Gene Synonym:
Exon (14)1878..1968
Gene:EGFR
Gene Synonym:
Exon (15)1969..2126
Gene:EGFR
Gene Synonym:
Exon (16)2127..2865
Gene:EGFR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_201284
Organism Homo sapiens (human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201284

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG GCCAGGAAAT GAGAGTCTCA AAGCCATGTT ATTCTGCCTT
TTTAAACTAT CATCCTGTAA TCAAAGTAAT GATGGCAGCG TGTCCCACCA GAGCGGGAGC
CCAGCTGCTC AGGAGTCATG CTTAGGATGG ATCCCTTCTC TTCTGCCGTC AGAGTTTCAG
CTGGGTTGGG GTGGATGCAG CCACCTCCAT GCCTGGCCTT CTGCATCTGT GATCATCACG
GCCTCCTCCT GCCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25437
Clone ID Related Accession (Same CDS sequence) NM_005228
Accession Version NM_005228.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product epidermal growth factor receptor isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW163038.1, X00588.1, AF125253.1, AU137334.1, CB160831.1, AL598260.1, AI217671.1 and AW295229.1. This sequence is a reference standard in the RefSeqGene project. On Jan 26, 2004 this sequence version replaced gi:29725608. Transcript Variant: This variant (1) is the longest transcript and it encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X00588.1, BC070081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACCG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005219.2
CDS247..3879
Misc Feature(1)208..210(+)
Misc Feature(2)337..420(+)
Misc Feature(3)412..414(+)
Misc Feature(4)415..750(+)
Misc Feature(5)469..1146(+)
Misc Feature(6)628..630(+)
Misc Feature(7)715..807(+)
Misc Feature(8)769..771(+)
Misc Feature(9)799..1257(+)
Misc Feature(10)814..843(+)
Misc Feature(11)826..867(+)
Misc Feature(12)832..834(+)
Misc Feature(13)889..915(+)
Misc Feature(14)901..939(+)
Misc Feature(15)931..933(+)
Misc Feature(16)937..1068(+)
Misc Feature(17)940..966(+)
Misc Feature(18)952..990(+)
Misc Feature(19)997..1026(+)
Misc Feature(20)1036..1119(+)
Misc Feature(21)1129..1167(+)
Misc Feature(22)1177..1224(+)
Misc Feature(23)1231..1245(+)
Misc Feature(24)1255..1332(+)
Misc Feature(25)1300..1302(+)
Misc Feature(26)1327..1689(+)
Misc Feature(27)1327..1329(+)
Misc Feature(28)1414..2046(+)
Misc Feature(29)1483..1485(+)
Misc Feature(30)1576..1578(+)
Misc Feature(31)1654..1743(+)
Misc Feature(32)1759..2157(+)
Misc Feature(33)1762..1923(+)
Misc Feature(34)1762..1791(+)
Misc Feature(35)1774..1815(+)
Misc Feature(36)1822..1851(+)
Misc Feature(37)1828..1830(+)
Misc Feature(38)1861..1911(+)
Misc Feature(39)1918..>2040(+)
Misc Feature(40)1918..1959(+)
Misc Feature(41)1930..1983(+)
Misc Feature(42)1948..1950(+)
Misc Feature(43)1990..2019(+)
Misc Feature(44)2029..2097(+)
Misc Feature(45)2053..2055(+)
Misc Feature(46)2104..2130(+)
Misc Feature(47)2113..2115(+)
Misc Feature(48)2116..2154(+)
Misc Feature(49)2146..2277(+)
Misc Feature(50)2176..2217(+)
Misc Feature(51)2182..2250(+)
Misc Feature(52)2278..2280(+)
Misc Feature(53)2278..2280(+)
Misc Feature(54)2308..2358(+)
Misc Feature(55)2323..2325(+)
Misc Feature(56)2323..2325(+)
Misc Feature(57)2329..2331(+)
Misc Feature(58)2356..3294(+)
Misc Feature(59)2380..3150(+)
Misc Feature(60)2389..3276(+)
Misc Feature(61)2398..2913(+)
Misc Feature(62)2398..2811(+)
Misc Feature(63)2425..2427(+)
Misc Feature(64)2548..2550(+)
Misc Feature(65)2806..2883(+)
Misc Feature(66)2851..2853(+)
Misc Feature(67)2872..2913(+)
Misc Feature(68)2917..2919(+)
Misc Feature(69)2989..2991(+)
Misc Feature(70)3076..3078(+)
Misc Feature(71)3178..3180(+)
Misc Feature(72)3217..3219(+)
Misc Feature(73)3217..3219(+)
Misc Feature(74)3229..3231(+)
Misc Feature(75)3229..3231(+)
Misc Feature(76)3238..3240(+)
Misc Feature(77)3238..3240(+)
Misc Feature(78)3292..3294(+)
Misc Feature(79)3292..3294(+)
Misc Feature(80)3292..3294(+)
Misc Feature(81)3292..3294(+)
Misc Feature(82)3292..3294(+)
Misc Feature(83)3322..3324(+)
Misc Feature(84)3322..3324(+)
Misc Feature(85)3352..3354(+)
Misc Feature(86)3355..3357(+)
Misc Feature(87)3361..3363(+)
Misc Feature(88)3361..3363(+)
Misc Feature(89)3367..3369(+)
Misc Feature(90)3367..3369(+)
Misc Feature(91)3370..3372(+)
Misc Feature(92)3370..3372(+)
Misc Feature(93)3379..3381(+)
Misc Feature(94)3436..3438(+)
Misc Feature(95)3436..3438(+)
Misc Feature(96)3451..3453(+)
Misc Feature(97)3451..3453(+)
Misc Feature(98)3451..3453(+)
Misc Feature(99)3451..3453(+)
Misc Feature(100)3451..3453(+)
Misc Feature(101)3454..3456(+)
Misc Feature(102)3454..3456(+)
Misc Feature(103)3454..3456(+)
Misc Feature(104)3457..3459(+)
Misc Feature(105)3457..3459(+)
Misc Feature(106)3457..3459(+)
Misc Feature(107)3487..3489(+)
Misc Feature(108)3487..3489(+)
Misc Feature(109)3520..3522(+)
Misc Feature(110)3520..3522(+)
Misc Feature(111)3520..3522(+)
Misc Feature(112)3574..3576(+)
Misc Feature(113)3574..3576(+)
Misc Feature(114)3604..3606(+)
Misc Feature(115)3619..3621(+)
Misc Feature(116)3658..3660(+)
Misc Feature(117)3742..3744(+)
Misc Feature(118)3742..3744(+)
Misc Feature(119)3760..3762(+)
Misc Feature(120)3760..3762(+)
Misc Feature(121)3835..3837(+)
Misc Feature(122)3835..3837(+)
Misc Feature(123)3835..3837(+)
Misc Feature(124)3841..3843(+)
Exon (1)1..334
Gene:EGFR
Gene Synonym:
Exon (2)335..486
Gene:EGFR
Gene Synonym:
Exon (3)487..670
Gene:EGFR
Gene Synonym:
Exon (4)671..805
Gene:EGFR
Gene Synonym:
Exon (5)806..874
Gene:EGFR
Gene Synonym:
Exon (6)875..993
Gene:EGFR
Gene Synonym:
Exon (7)994..1135
Gene:EGFR
Gene Synonym:
Exon (8)1136..1252
Gene:EGFR
Gene Synonym:
Exon (9)1253..1379
Gene:EGFR
Gene Synonym:
Exon (10)1380..1453
Gene:EGFR
Gene Synonym:
Exon (11)1454..1544
Gene:EGFR
Gene Synonym:
Exon (12)1545..1744
Gene:EGFR
Gene Synonym:
Exon (13)1745..1877
Gene:EGFR
Gene Synonym:
Exon (14)1878..1968
Gene:EGFR
Gene Synonym:
Exon (15)1969..2126
Gene:EGFR
Gene Synonym:
Exon (16)2127..2165
Gene:EGFR
Gene Synonym:
Exon (17)2166..2307
Gene:EGFR
Gene Synonym:
Exon (18)2308..2430
Gene:EGFR
Gene Synonym:
Exon (19)2431..2529
Gene:EGFR
Gene Synonym:
Exon (20)2530..2715
Gene:EGFR
Gene Synonym:
Exon (21)2716..2871
Gene:EGFR
Gene Synonym:
Exon (22)2872..2947
Gene:EGFR
Gene Synonym:
Exon (23)2948..3094
Gene:EGFR
Gene Synonym:
Exon (24)3095..3192
Gene:EGFR
Gene Synonym:
Exon (25)3193..3360
Gene:EGFR
Gene Synonym:
Exon (26)3361..3408
Gene:EGFR
Gene Synonym:
Exon (27)3409..3517
Gene:EGFR
Gene Synonym:
Exon (28)3518..5600
Gene:EGFR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005228
Organism Homo sapiens (human)
Definition Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005228

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCG 
GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA GCTCACGCAG
TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG
GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG
ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA GCGAATTCCT
TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA TGCCTTAGCA
GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAATTTA
CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG CAACGTGGAG
AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC GATGGACTTC
CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG GAGCTGCTGG
GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA GCAGTGCTCC
GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC TGCAGGCTGC
ACAGGCCCCC GGGAGAGCGA CTGCCTGGTC TGCCGCAAAT TCCGAGACGA AGCCACGTGC
AAGGACACCT GCCCCCCACT CATGCTCTAC AACCCCACCA CGTACCAGAT GGATGTGAAC
CCCGAGGGCA AATACAGCTT TGGTGCCACC TGCGTGAAGA AGTGTCCCCG TAATTATGTG
GTGACAGATC ACGGCTCGTG CGTCCGAGCC TGTGGGGCCG ACAGCTATGA GATGGAGGAA
GACGGCGTCC GCAAGTGTAA GAAGTGCGAA GGGCCTTGCC GCAAAGTGTG TAACGGAATA
GGTATTGGTG AATTTAAAGA CTCACTCTCC ATAAATGCTA CGAATATTAA ACACTTCAAA
AACTGCACCT CCATCAGTGG CGATCTCCAC ATCCTGCCGG TGGCATTTAG GGGTGACTCC
TTCACACATA CTCCTCCTCT GGATCCACAG GAACTGGATA TTCTGAAAAC CGTAAAGGAA
ATCACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACA GGACGGACCT CCATGCCTTT
GAGAACCTAG AAATCATACG CGGCAGGACC AAGCAACATG GTCAGTTTTC TCTTGCAGTC
GTCAGCCTGA ACATAACATC CTTGGGATTA CGCTCCCTCA AGGAGATAAG TGATGGAGAT
GTGATAATTT CAGGAAACAA AAATTTGTGC TATGCAAATA CAATAAACTG GAAAAAACTG
TTTGGGACCT CCGGTCAGAA AACCAAAATT ATAAGCAACA GAGGTGAAAA CAGCTGCAAG
GCCACAGGCC AGGTCTGCCA TGCCTTGTGC TCCCCCGAGG GCTGCTGGGG CCCGGAGCCC
AGGGACTGCG TCTCTTGCCG GAATGTCAGC CGAGGCAGGG AATGCGTGGA CAAGTGCAAC
CTTCTGGAGG GTGAGCCAAG GGAGTTTGTG GAGAACTCTG AGTGCATACA GTGCCACCCA
GAGTGCCTGC CTCAGGCCAT GAACATCACC TGCACAGGAC GGGGACCAGA CAACTGTATC
CAGTGTGCCC ACTACATTGA CGGCCCCCAC TGCGTCAAGA CCTGCCCGGC AGGAGTCATG
GGAGAAAACA ACACCCTGGT CTGGAAGTAC GCAGACGCCG GCCATGTGTG CCACCTGTGC
CATCCAAACT GCACCTACGG ATGCACTGGG CCAGGTCTTG AAGGCTGTCC AACGAATGGG
CCTAAGATCC CGTCCATCGC CACTGGGATG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
GCCCTGGGGA TCGGCCTCTT CATGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
CCCGTCGCTA TCAAGGAATT AAGAGAAGCA ACATCTCCGA AAGCCAACAA GGAAATCCTC
GATGAAGCCT ACGTGATGGC CAGCGTGGAC AACCCCCACG TGTGCCGCCT GCTGGGCATC
TGCCTCACCT CCACCGTGCA GCTCATCACG CAGCTCATGC CCTTCGGCTG CCTCCTGGAC
TATGTCCGGG AACACAAAGA CAATATTGGC TCCCAGTACC TGCTCAACTG GTGTGTGCAG
ATCGCAAAGG GCATGAACTA CTTGGAGGAC CGTCGCTTGG TGCACCGCGA CCTGGCAGCC
AGGAACGTAC TGGTGAAAAC ACCGCAGCAT GTCAAGATCA CAGATTTTGG GCTGGCCAAA
CTGCTGGGTG CGGAAGAGAA AGAATACCAT GCAGAAGGAG GCAAAGTGCC TATCAAGTGG
ATGGCATTGG AATCAATTTT ACACAGAATC TATACCCACC AGAGTGATGT CTGGAGCTAC
GGGGTGACCG TTTGGGAGTT GATGACCTTT GGATCCAAGC CATATGACGG AATCCCTGCC
AGCGAGATCT CCTCCATCCT GGAGAAAGGA GAACGCCTCC CTCAGCCACC CATATGTACC
ATCGATGTCT ACATGATCAT GGTCAAGTGC TGGATGATAG ACGCAGATAG TCGCCCAAAG
TTCCGTGAGT TGATCATCGA ATTCTCCAAA ATGGCCCGAG ACCCCCAGCG CTACCTTGTC
ATTCAGGGGG ATGAAAGAAT GCATTTGCCA AGTCCTACAG ACTCCAACTT CTACCGTGCC
CTGATGGATG AAGAAGACAT GGACGACGTG GTGGATGCCG ACGAGTACCT CATCCCACAG
CAGGGCTTCT TCAGCAGCCC CTCCACGTCA CGGACTCCCC TCCTGAGCTC TCTGAGTGCA
ACCAGCAACA ATTCCACCGT GGCTTGCATT GATAGAAATG GGCTGCAAAG CTGTCCCATC
AAGGAAGACA GCTTCTTGCA GCGATACAGC TCAGACCCCA CAGGCGCCTT GACTGAGGAC
AGCATAGACG ACACCTTCCT CCCAGTGCCT GAATACATAA ACCAGTCCGT TCCCAAAAGG
CCCGCTGGCT CTGTGCAGAA TCCTGTCTAT CACAATCAGC CTCTGAACCC CGCGCCCAGC
AGAGACCCAC ACTACCAGGA CCCCCACAGC ACTGCAGTGG GCAACCCCGA GTATCTCAAC
ACTGTCCAGC CCACCTGTGT CAACAGCACA TTCGACAGCC CTGCCCACTG GGCCCAGAAA
GGCAGCCACC AAATTAGCCT GGACAACCCT GACTACCAGC AGGACTTCTT TCCCAAGGAA
GCCAAGCCAA ATGGCATCTT TAAGGGCTCC ACAGCTGAAA ATGCAGAATA CCTAAGGGTC
GCGCCACAAA GCAGTGAATT TATTGGAGCA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

TD-19, an erlotinib derivative, induces epidermal growth factor receptor wild-type nonsmall-cell lung cancer apoptosis through CIP2A-mediated pathway
J. Pharmacol. Exp. Ther. 351 (2), 352-358 (2014)
Chao TT, Wang CY, Lai CC, Chen YL, Tsai YT, Chen PT, Lin HI, Huang YC, Shiau CW, Yu CJ and Chen KF.


book

Head and neck squamous cell carcinomas do not express EGFRvIII
Int. J. Radiat. Oncol. Biol. Phys. 90 (2), 454-462 (2014)
Melchers LJ, Clausen MJ, Mastik MF, Slagter-Menkema L, Langendijk JA, van der Laan BF, van der Wal JE, van der Vegt B, Roodenburg JL and Schuuring E.


book

EGFR has a tumour-promoting role in liver macrophages during hepatocellular carcinoma formation
Nat. Cell Biol. 16 (10), 972-981 (2014)
Lanaya H, Natarajan A, Komposch K, Li L, Amberg N, Chen L, Wculek SK, Hammer M, Zenz R, Peck-Radosavljevic M, Sieghart W, Trauner M, Wang H and Sibilia M.


book

Epidermal growth factor receptor (EGFR) mutation testing, from bench to practice: a single institute experience
Ir Med J 107 (7), 201-204 (2014)
Shikhrakab,H., Elamin,Y.Y., O'Brien,C., Gately,K., Finn,S., O'Byrne,K. and Osman,N.


book

Non-invasive detection of EGFR deletion at exon 19 in non-small cell lung cancer by real time diagnostic
Clin. Lab. 60 (9), 1517-1526 (2014)
Sun,H., Gan,Z.C., Gao,J.J. and Zheng,F.


book

The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors
Mol. Cell. Biol. 12 (12), 5824-5833 (1992)
Li W, Hu P, Skolnik EY, Ullrich A and Schlessinger J.


book

Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin
J. Biol. Chem. 267 (27), 19258-19265 (1992)
Krieg J and Hunter T.


book

The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling
Cell 70 (3), 431-442 (1992)
Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D and Schlessinger J.


book

Two chromosome 7 dinucleotide repeat polymorphisms at gene loci epidermal growth factor receptor (EGFR) and pro alpha 2 (I) collagen (COL1A2)
Hum. Mol. Genet. 1 (2), 135 (1992)
Chi DD, Hing AV, Helms C, Steinbrueck T, Mishra SK and Donis-Keller H.


book

Mechanism of desensitization of the epidermal growth factor receptor protein-tyrosine kinase
J. Biol. Chem. 267 (2), 1129-1140 (1992)
Countaway JL, Nairn AC and Davis RJ.


 
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