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ACSF3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ACSF3
Entrez Gene ID 197322
Full Name acyl-CoA synthetase family member 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q24.3

Summary This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

614245


mRNA and Protein(s)

mRNA Protein Name
NM_001127214 NP_001120686 acyl-CoA synthetase family member 3, mitochondrial isoform 1 precursor
NM_001243279 NP_001230208 acyl-CoA synthetase family member 3, mitochondrial isoform 1 precursor
NM_001284316 NP_001271245 acyl-CoA synthetase family member 3, mitochondrial isoform 2
NM_174917 NP_777577 acyl-CoA synthetase family member 3, mitochondrial isoform 1 precursor
XM_005256293 XP_005256350 acyl-CoA synthetase family member 3, mitochondrial isoform X1
XM_011522942 XP_011521244 acyl-CoA synthetase family member 3, mitochondrial isoform X1
XM_011522943 XP_011521245 acyl-CoA synthetase family member 3, mitochondrial isoform X1
XM_011522944 XP_011521246 acyl-CoA synthetase family member 3, mitochondrial isoform X2



Homo sapiens (human) ACSF3 NP_777577.2
Pan troglodytes (chimpanzee) ACSF3 XP_511166.3
Canis lupus familiaris (dog) ACSF3 XP_005620620.1
Bos taurus (cattle) ACSF3 NP_001030240.1
Mus musculus (house mouse) Acsf3 NP_659181.2
Rattus norvegicus (Norway rat) Acsf3 XP_574249.3
Gallus gallus (chicken) ACSF3 XP_004944364.1
Danio rerio (zebrafish) LOC562292 XP_690782.2
Arabidopsis thaliana (thale cress) AT3G16170 NP_566537.1
Xenopus (Silurana) tropicalis (western clawed frog) acsf3 NP_001121382.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACSF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu01574
NM_001127214 Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu01574
NM_001243279 Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu08539 NM_001284316 Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu01574
NM_174917 Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu49300 XM_005256293 PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu49300 XM_011522942 PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu49300 XM_011522943 PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu77541 XM_011522944 PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu01574
Clone ID Related Accession (Same CDS sequence) NM_001127214 , NM_001243279 , NM_174917
Accession Version NM_001127214.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM148793.1, AK290963.1 and AC009113.8. On Sep 27, 2013 this sequence version replaced gi:343168764. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK290963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGAAATGTC
CTGGCCCCGT ACGCGGTGCC CTCGGAGCTG GTGCTGGTGG AGGAGATCCC GCGGAACCAG
ATGGGCAAGA TTGACAAGAA GGCGCTCATC AGGCACTTCC ACCCCTCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120686.1
CDS209..1939
Misc Feature(1)350..1915(+)
Misc Feature(2)371..1906(+)
Misc Feature(3)803..838(+)
Misc Feature(4)812..1852(+)
Misc Feature(5)812..1657(+)
Misc Feature(6)932..1852(+)
Exon (1)1..188
Gene:ACSF3
Gene Synonym:
Exon (2)189..874
Gene:ACSF3
Gene Synonym:
Exon (3)875..1030
Gene:ACSF3
Gene Synonym:
Exon (4)1031..1185
Gene:ACSF3
Gene Synonym:
Exon (5)1186..1334
Gene:ACSF3
Gene Synonym:
Exon (6)1335..1447
Gene:ACSF3
Gene Synonym:
Exon (7)1448..1574
Gene:ACSF3
Gene Synonym:
Exon (8)1575..1709
Gene:ACSF3
Gene Synonym:
Exon (9)1710..1821
Gene:ACSF3
Gene Synonym:
Exon (10)1822..3578
Gene:ACSF3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127214
Organism Homo sapiens (human)
Definition Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127214

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGAAATGTC
CTGGCCCCGT ACGCGGTGCC CTCGGAGCTG GTGCTGGTGG AGGAGATCCC GCGGAACCAG
ATGGGCAAGA TTGACAAGAA GGCGCTCATC AGGCACTTCC ACCCCTCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01574
Clone ID Related Accession (Same CDS sequence) NM_001127214 , NM_001243279 , NM_174917
Accession Version NM_001243279.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM148793.1, BC028399.1, AC135782.4 and AC009113.8. On Sep 27, 2013 this sequence version replaced gi:343168766. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC028399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGAAATGTC
CTGGCCCCGT ACGCGGTGCC CTCGGAGCTG GTGCTGGTGG AGGAGATCCC GCGGAACCAG
ATGGGCAAGA TTGACAAGAA GGCGCTCATC AGGCACTTCC ACCCCTCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230208.1
CDS382..2112
Misc Feature(1)289..291(+)
Misc Feature(2)523..2088(+)
Misc Feature(3)544..2079(+)
Misc Feature(4)976..1011(+)
Misc Feature(5)985..2025(+)
Misc Feature(6)985..1830(+)
Misc Feature(7)1105..2025(+)
Exon (1)1..188
Gene:ACSF3
Gene Synonym:
Exon (2)189..361
Gene:ACSF3
Gene Synonym:
Exon (3)362..1047
Gene:ACSF3
Gene Synonym:
Exon (4)1048..1203
Gene:ACSF3
Gene Synonym:
Exon (5)1204..1358
Gene:ACSF3
Gene Synonym:
Exon (6)1359..1507
Gene:ACSF3
Gene Synonym:
Exon (7)1508..1620
Gene:ACSF3
Gene Synonym:
Exon (8)1621..1747
Gene:ACSF3
Gene Synonym:
Exon (9)1748..1882
Gene:ACSF3
Gene Synonym:
Exon (10)1883..1994
Gene:ACSF3
Gene Synonym:
Exon (11)1995..3751
Gene:ACSF3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243279
Organism Homo sapiens (human)
Definition Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243279

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGAAATGTC
CTGGCCCCGT ACGCGGTGCC CTCGGAGCTG GTGCTGGTGG AGGAGATCCC GCGGAACCAG
ATGGGCAAGA TTGACAAGAA GGCGCTCATC AGGCACTTCC ACCCCTCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu08539
Clone ID Related Accession (Same CDS sequence) NM_001284316
Accession Version NM_001284316.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 936bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM148793.1, BX402632.2, BQ953430.1, BX325026.2, AC009113.8 and BI837487.1. On Sep 27, 2013 this sequence version replaced gi:343168763. Transcript Variant: This variant (3) lacks two exons, one of which contains a portion of the 5' UTR and the other which contains a portion of the 5' coding region including the start codon, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC064609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGATGCCTG AGTTCAGCCC TCAGCAGGTT TGGGAAAAGT TCTTAAGTTC TGAAACGCCG 
CGGATCAATG TCTTTATGGC AGTGCCTACA ATATACACCA AGCTGATGGA GTACTACGAC
AGGCATTTTA CCCAGCCGCA CGCCCAGGAT TTCTTGCGTG CAGTTTGTGA AGAAAAAATT
AGGCTGATGG TCTCAGGCTC AGCTGCCCTG CCCCTCCCAG TGCTGGAGAA GTGGAAGAAC
ATCACGGGCC ACACCCTGCT GGAGCGGTAT GGCATGACCG AGATCGGCAT GGCTCTGTCC
GGGCCCCTGA CCACTGCCGT GCGCCTGCCA GGTTCCGTGG GGACCCCACT GCCTGGAGTA
CAGGTGCGCA TTGTCTCAGA AAACCCACAG AGGGAAGCCT GCTCCTACAC CATCCACGCA
GAGGGAGACG AGAGGGGGAC CAAGGTGACC CCAGGGTTTG AAGAAAAGGA GGGGGAGCTG
CTGGTGAGGG GACCCTCCGT GTTTCGAGAA TACTGGAATA AACCAGAAGA AACTAAGAGT
GCATTCACCC TGGATGGCTG GTTTAAGACA GGGGACACCG TGGTGTTTAA GGATGGCCAG
TACTGGATCC GAGGCCGGAC CTCAGTGGAC ATCATCAAGA CTGGAGGCTA CAAGGTCAGC
GCCCTGGAGG TGGAGTGGCA CCTGCTGGCC CACCCCAGCA TCACAGATGT GGCTGTGATT
GGAGTTCCGG ATATGACATG GGGCCAGCGG GTCACTGCTG TGGTGACCCT CCGAGAAGGA
CACTCACTGT CCCACAGGGA GCTCAAAGAG TGGGCCAGAA ATGTCCTGGC CCCGTACGCG
GTGCCCTCGG AGCTGGTGCT GGTGGAGGAG ATCCCGCGGA ACCAGATGGG CAAGATTGAC
AAGAAGGCGC TCATCAGGCA CTTCCACCCC TCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001271245.1
CDS314..1249
Misc Feature(1)314..1216(+)
Misc Feature(2)326..1225(+)
Misc Feature(3)395..1150(+)
Misc Feature(4)401..1150(+)
Misc Feature(5)509..1207(+)
Exon (1)1..184
Gene:ACSF3
Gene Synonym:
Exon (2)185..340
Gene:ACSF3
Gene Synonym:
Exon (3)341..495
Gene:ACSF3
Gene Synonym:
Exon (4)496..644
Gene:ACSF3
Gene Synonym:
Exon (5)645..757
Gene:ACSF3
Gene Synonym:
Exon (6)758..884
Gene:ACSF3
Gene Synonym:
Exon (7)885..1019
Gene:ACSF3
Gene Synonym:
Exon (8)1020..1131
Gene:ACSF3
Gene Synonym:
Exon (9)1132..2888
Gene:ACSF3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001284316
Organism Homo sapiens (human)
Definition Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001284316

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGATGCCTG AGTTCAGCCC TCAGCAGGTT TGGGAAAAGT TCTTAAGTTC TGAAACGCCG 
CGGATCAATG TCTTTATGGC AGTGCCTACA ATATACACCA AGCTGATGGA GTACTACGAC
AGGCATTTTA CCCAGCCGCA CGCCCAGGAT TTCTTGCGTG CAGTTTGTGA AGAAAAAATT
AGGCTGATGG TCTCAGGCTC AGCTGCCCTG CCCCTCCCAG TGCTGGAGAA GTGGAAGAAC
ATCACGGGCC ACACCCTGCT GGAGCGGTAT GGCATGACCG AGATCGGCAT GGCTCTGTCC
GGGCCCCTGA CCACTGCCGT GCGCCTGCCA GGTTCCGTGG GGACCCCACT GCCTGGAGTA
CAGGTGCGCA TTGTCTCAGA AAACCCACAG AGGGAAGCCT GCTCCTACAC CATCCACGCA
GAGGGAGACG AGAGGGGGAC CAAGGTGACC CCAGGGTTTG AAGAAAAGGA GGGGGAGCTG
CTGGTGAGGG GACCCTCCGT GTTTCGAGAA TACTGGAATA AACCAGAAGA AACTAAGAGT
GCATTCACCC TGGATGGCTG GTTTAAGACA GGGGACACCG TGGTGTTTAA GGATGGCCAG
TACTGGATCC GAGGCCGGAC CTCAGTGGAC ATCATCAAGA CTGGAGGCTA CAAGGTCAGC
GCCCTGGAGG TGGAGTGGCA CCTGCTGGCC CACCCCAGCA TCACAGATGT GGCTGTGATT
GGAGTTCCGG ATATGACATG GGGCCAGCGG GTCACTGCTG TGGTGACCCT CCGAGAAGGA
CACTCACTGT CCCACAGGGA GCTCAAAGAG TGGGCCAGAA ATGTCCTGGC CCCGTACGCG
GTGCCCTCGG AGCTGGTGCT GGTGGAGGAG ATCCCGCGGA ACCAGATGGG CAAGATTGAC
AAGAAGGCGC TCATCAGGCA CTTCCACCCC TCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01574
Clone ID Related Accession (Same CDS sequence) NM_001127214 , NM_001243279 , NM_174917
Accession Version NM_174917.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM148793.1, DA062861.1, AK075499.1, AC135782.4 and AC009113.8. This sequence is a reference standard in the RefSeqGene project. On Sep 27, 2013 this sequence version replaced gi:343168765. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 2 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK075499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGAAATGTC
CTGGCCCCGT ACGCGGTGCC CTCGGAGCTG GTGCTGGTGG AGGAGATCCC GCGGAACCAG
ATGGGCAAGA TTGACAAGAA GGCGCTCATC AGGCACTTCC ACCCCTCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_777577.2
CDS378..2108
Misc Feature(1)285..287(+)
Misc Feature(2)519..2084(+)
Misc Feature(3)540..2075(+)
Misc Feature(4)972..1007(+)
Misc Feature(5)981..2021(+)
Misc Feature(6)981..1826(+)
Misc Feature(7)1101..2021(+)
Exon (1)1..184
Gene:ACSF3
Gene Synonym:
Exon (2)185..357
Gene:ACSF3
Gene Synonym:
Exon (3)358..1043
Gene:ACSF3
Gene Synonym:
Exon (4)1044..1199
Gene:ACSF3
Gene Synonym:
Exon (5)1200..1354
Gene:ACSF3
Gene Synonym:
Exon (6)1355..1503
Gene:ACSF3
Gene Synonym:
Exon (7)1504..1616
Gene:ACSF3
Gene Synonym:
Exon (8)1617..1743
Gene:ACSF3
Gene Synonym:
Exon (9)1744..1878
Gene:ACSF3
Gene Synonym:
Exon (10)1879..1990
Gene:ACSF3
Gene Synonym:
Exon (11)1991..3747
Gene:ACSF3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_174917
Organism Homo sapiens (human)
Definition Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_174917

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGAAATGTC
CTGGCCCCGT ACGCGGTGCC CTCGGAGCTG GTGCTGGTGG AGGAGATCCC GCGGAACCAG
ATGGGCAAGA TTGACAAGAA GGCGCTCATC AGGCACTTCC ACCCCTCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49300
Clone ID Related Accession (Same CDS sequence) XM_005256293 , XM_011522942 , XM_011522943
Accession Version XM_005256293.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1833bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGGTTGAAA
GAACACCCAA CCAAGAGGAG AGAAAACAGC ATTCTAAATA TCGGAAGGAA ACGAAACCTC
GCCTCAGGCA TGCGGGCTCT GACGGGCAGG ATGGCGGCAC GGAGGGCTGC TGTATCCAGT
GAAGTCAGCA GTTCAGATGA AACAGATAAC TCCCTTAAAA GACACAAATT GCCAAGACTG
ACGCAAACAC TGAAACAGAC TGAACAGTTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256350.1
CDS345..2177
Misc Feature(1)480..1784(+)
Misc Feature(2)507..1982(+)
Misc Feature(3)939..974(+)
Misc Feature(4)948..1880(+)
Misc Feature(5)948..1793(+)
Misc Feature(6)1068..1880(+)
Translation

Target ORF information:

RefSeq Version XM_005256293
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005256293

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGGTTGAAA
GAACACCCAA CCAAGAGGAG AGAAAACAGC ATTCTAAATA TCGGAAGGAA ACGAAACCTC
GCCTCAGGCA TGCGGGCTCT GACGGGCAGG ATGGCGGCAC GGAGGGCTGC TGTATCCAGT
GAAGTCAGCA GTTCAGATGA AACAGATAAC TCCCTTAAAA GACACAAATT GCCAAGACTG
ACGCAAACAC TGAAACAGAC TGAACAGTTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49300
Clone ID Related Accession (Same CDS sequence) XM_005256293 , XM_011522942 , XM_011522943
Accession Version XM_011522942.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1833bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGGTTGAAA
GAACACCCAA CCAAGAGGAG AGAAAACAGC ATTCTAAATA TCGGAAGGAA ACGAAACCTC
GCCTCAGGCA TGCGGGCTCT GACGGGCAGG ATGGCGGCAC GGAGGGCTGC TGTATCCAGT
GAAGTCAGCA GTTCAGATGA AACAGATAAC TCCCTTAAAA GACACAAATT GCCAAGACTG
ACGCAAACAC TGAAACAGAC TGAACAGTTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521244.1
CDS322..2154
Misc Feature(1)457..1761(+)
Misc Feature(2)484..1959(+)
Misc Feature(3)916..951(+)
Misc Feature(4)925..1857(+)
Misc Feature(5)925..1770(+)
Misc Feature(6)1045..1857(+)
Translation

Target ORF information:

RefSeq Version XM_011522942
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522942

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGGTTGAAA
GAACACCCAA CCAAGAGGAG AGAAAACAGC ATTCTAAATA TCGGAAGGAA ACGAAACCTC
GCCTCAGGCA TGCGGGCTCT GACGGGCAGG ATGGCGGCAC GGAGGGCTGC TGTATCCAGT
GAAGTCAGCA GTTCAGATGA AACAGATAAC TCCCTTAAAA GACACAAATT GCCAAGACTG
ACGCAAACAC TGAAACAGAC TGAACAGTTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49300
Clone ID Related Accession (Same CDS sequence) XM_005256293 , XM_011522942 , XM_011522943
Accession Version XM_011522943.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1833bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGGTTGAAA
GAACACCCAA CCAAGAGGAG AGAAAACAGC ATTCTAAATA TCGGAAGGAA ACGAAACCTC
GCCTCAGGCA TGCGGGCTCT GACGGGCAGG ATGGCGGCAC GGAGGGCTGC TGTATCCAGT
GAAGTCAGCA GTTCAGATGA AACAGATAAC TCCCTTAAAA GACACAAATT GCCAAGACTG
ACGCAAACAC TGAAACAGAC TGAACAGTTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521245.1
CDS169..2001
Misc Feature(1)304..1608(+)
Misc Feature(2)331..1806(+)
Misc Feature(3)763..798(+)
Misc Feature(4)772..1704(+)
Misc Feature(5)772..1617(+)
Misc Feature(6)892..1704(+)
Translation

Target ORF information:

RefSeq Version XM_011522943
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522943

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGGTTC CGTGGGGACC
CCACTGCCTG GAGTACAGGT GCGCATTGTC TCAGAAAACC CACAGAGGGA AGCCTGCTCC
TACACCATCC ACGCAGAGGG AGACGAGAGG GGGACCAAGG TGACCCCAGG GTTTGAAGAA
AAGGAGGGGG AGCTGCTGGT GAGGGGACCC TCCGTGTTTC GAGAATACTG GAATAAACCA
GAAGAAACTA AGAGTGCATT CACCCTGGAT GGCTGGTTTA AGACAGGGGA CACCGTGGTG
TTTAAGGATG GCCAGTACTG GATCCGAGGC CGGACCTCAG TGGACATCAT CAAGACTGGA
GGCTACAAGG TCAGCGCCCT GGAGGTGGAG TGGCACCTGC TGGCCCACCC CAGCATCACA
GATGTGGCTG TGATTGGAGT TCCGGATATG ACATGGGGCC AGCGGGTCAC TGCTGTGGTG
ACCCTCCGAG AAGGACACTC ACTGTCCCAC AGGGAGCTCA AAGAGTGGGC CAGGTTGAAA
GAACACCCAA CCAAGAGGAG AGAAAACAGC ATTCTAAATA TCGGAAGGAA ACGAAACCTC
GCCTCAGGCA TGCGGGCTCT GACGGGCAGG ATGGCGGCAC GGAGGGCTGC TGTATCCAGT
GAAGTCAGCA GTTCAGATGA AACAGATAAC TCCCTTAAAA GACACAAATT GCCAAGACTG
ACGCAAACAC TGAAACAGAC TGAACAGTTC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu77541
Clone ID Related Accession (Same CDS sequence) XM_011522944
Accession Version XM_011522944.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1344bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acyl-CoA synthetase family member 3, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGTAGG ATGTGCTGGT
TTTGTCCAGT GCCACCGGCC ACAGTCACCA CGTAATATGG ACGGAATGAG CAAAGCCCGG
CTCGCTGAGG CTGCAGGCTG CAGCATTGCT TTGTGGGCGT CCTCTCTTTT GATCTTCTCG
AAACAGCCCC TGCGATGGGG TCATATTTTG CACTCACATA CAAGAACCCA TGAGCCTGCC
CACGGTCACG CGGCTGGTGA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521246.1
CDS345..1688
Misc Feature(1)480..>1484(+)
Misc Feature(2)507..>1475(+)
Misc Feature(3)939..974(+)
Misc Feature(4)948..1418(+)
Misc Feature(5)948..1418(+)
Misc Feature(6)1068..1337(+)
Translation

Target ORF information:

RefSeq Version XM_011522944
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522944

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCTGCCCC ATGTGGTGCT CACCTTCCGG CGCCTGGGCT GCGCCTTGGC GTCCTGCCGG 
CTGGCGCCTG CGAGACACAG AGGAAGTGGT CTTCTGCACA CAGCCCCAGT GGCCCGCTCG
GACAGGAGCG CCCCGGTGTT CACCCGTGCC CTGGCCTTTG GGGACAGAAT CGCCCTGGTT
GACCAGCACG GCCGCCACAC GTACAGGGAG CTTTATTCCC GCAGCCTTCG CCTGTCCCAG
GAGATCTGCA GGCTCTGCGG GTGTGTCGGC GGGGACCTCC GGGAGGAGAG GGTCTCCTTC
CTATGCGCTA ACGATGCCTC CTACGTCGTG GCCCAGTGGG CGTCATGGAT GAGTGGCGGT
GTGGCAGTCC CCCTCTACAG GAAGCATCCC GCGGCCCAGC TGGAGTATGT CATCTGCGAC
TCCCAGAGCT CTGTGGTCCT TGCCAGCCAG GAGTACCTGG AGCTCCTGAG CCCGGTGGTC
AGGAAGCTGG GGGTCCCGCT GCTGCCGCTC ACACCAGCCA TCTACACTGG AGCAGTAGAG
GAACCGGCAG AGGTCCCGGT CCCAGAGCAG GGATGGAGGA ACAAGGGCGC CATGATCATC
TACACCAGTG GGACCACGGG GAGGCCCAAG GGCGTGCTGA GCACGCACCA AAACATCAGG
GCTGTGGTGA CCGGGCTGGT CCACAAGTGG GCATGGACCA AAGACGACGT GATCCTCCAC
GTGCTCCCGC TGCACCACGT CCATGGTGTG GTCAACGCGC TGCTCTGTCC TCTCTGGGTG
GGAGCCACCT GTGTGATGAT GCCTGAGTTC AGCCCTCAGC AGGTTTGGGA AAAGTTCTTA
AGTTCTGAAA CGCCGCGGAT CAATGTCTTT ATGGCAGTGC CTACAATATA CACCAAGCTG
ATGGAGTACT ACGACAGGCA TTTTACCCAG CCGCACGCCC AGGATTTCTT GCGTGCAGTT
TGTGAAGAAA AAATTAGGCT GATGGTCTCA GGCTCAGCTG CCCTGCCCCT CCCAGTGCTG
GAGAAGTGGA AGAACATCAC GGGCCACACC CTGCTGGAGC GGTATGGCAT GACCGAGATC
GGCATGGCTC TGTCCGGGCC CCTGACCACT GCCGTGCGCC TGCCAGTAGG ATGTGCTGGT
TTTGTCCAGT GCCACCGGCC ACAGTCACCA CGTAATATGG ACGGAATGAG CAAAGCCCGG
CTCGCTGAGG CTGCAGGCTG CAGCATTGCT TTGTGGGCGT CCTCTCTTTT GATCTTCTCG
AAACAGCCCC TGCGATGGGG TCATATTTTG CACTCACATA CAAGAACCCA TGAGCCTGCC
CACGGTCACG CGGCTGGTGA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Analysis of differentially expressed genes and microRNAs in alcoholic liver disease
Int. J. Mol. Med. 31 (3), 547-554 (2013)
Liu Y, Chen SH, Jin X and Li YM.


book

The CBFA2T3/ACSF3 locus is recurrently involved in IGH chromosomal translocation t(14;16)(q32;q24) in pediatric B-cell lymphoma with germinal center phenotype
Genes Chromosomes Cancer 51 (4), 338-343 (2012)
Salaverria I, Akasaka T, Gesk S, Szczepanowski M, Burkhardt B, Harder L, Damm-Welk C, Oschlies I, Klapper W, Dyer MJ and Siebert R.


book

Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis
J. Biol. Chem. 286 (39), 33729-33736 (2011)
Witkowski A, Thweatt J and Smith S.


book

Exome sequencing identifies ACSF3 as a cause of combined malonic and methylmalonic aciduria
Nat. Genet. 43 (9), 883-886 (2011)
Sloan JL, Johnston JJ, Manoli I, Chandler RJ, Krause C, Carrillo-Carrasco N, Chandrasekaran SD, Sysol JR, O'Brien K, Hauser NS, Sapp JC, Dorward HM, Huizing M, Barshop BA, Berry SA, James PM, Champaigne NL, de Lonlay P, Valayannopoulos V, Geschwind MD, Gavrilov DK, Nyhan WL, Biesecker LG and Venditti CP.


book

Combined malonic and methylmalonic aciduria: exome sequencing reveals mutations in the ACSF3 gene in patients with a non-classic phenotype
J. Med. Genet. 48 (9), 602-605 (2011)
Alfares A, Nunez LD, Al-Thihli K, Mitchell J, Melancon S, Anastasio N, Ha KC, Majewski J, Rosenblatt DS and Braverman N.


book

Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome
J. Lipid Res. 48 (12), 2736-2750 (2007)
Watkins PA, Maiguel D, Jia Z and Pevsner J.


 
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