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EN1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EN1
Entrez Gene ID 2019
Full Name engrailed homeobox 1
General protein information
Preferred Names
homeobox protein engrailed-1
Names
homeobox protein engrailed-1
hu-En-1
engrailed homolog 1
homeobox protein en-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q14.2

Summary Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

131290


mRNA and Protein(s)

mRNA Protein Name
NM_001426 NP_001417 homeobox protein engrailed-1



Homo sapiens (human) EN1 NP_001417.3
Pan troglodytes (chimpanzee) EN1 XP_001172516.1
Bos taurus (cattle) EN1 XP_003585804.1
Mus musculus (house mouse) En1 NP_034263.2
Rattus norvegicus (Norway rat) En1 XP_001056699.2
Danio rerio (zebrafish) eng1b NP_001013516.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18410
NM_001426 Homo sapiens engrailed homeobox 1 (EN1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18410
Clone ID Related Accession (Same CDS sequence) NM_001426
Accession Version NM_001426.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homeobox protein engrailed-1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012665.8. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced gi:7710118. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAAGAAC AGCAGCCGGA ACCTAAAAGT CAGCGCGACT CGGCCCTCGG CGCGGCGGCG 
GCGGCGACTC CGGGCGGCCT CAGCCTGAGC CTCAGTCCGG GCGCCAGCGG CAGCAGCGGC
AGCGGCAGCG ATGGAGACAG CGTGCCGGTG TCCCCGCAGC CTGCGCCCCC CTCGCCGCCC
GCGGCGCCTT GCCTGCCGCC CCTGGCCCAC CACCCGCACC TCCCCCCACA CCCCCCGCCC
CCGCCGCCTC AGCATCTCGC GGCGCCTGCT CACCAGCCGC AGCCAGCGGC CCAGCTGCAC
CGCACCACCA ACTTTTTCAT CGACAACATC CTGAGGCCGG ACTTCGGCTG CAAAAAGGAG
CAGCCGCCAC CGCAGCTTCT GGTGGCTGCG GCGGCCAGAG GAGGCGCAGG AGGAGGAGGC
CGGGTCGAGC GTGACAGAGG CCAGACTGCC GCAGGTAGAG ACCCTGTCCA CCCGTTGGGC
ACCCGGGCGC CAGGCGCTGC CTCGCTCCTG TGCGCCCCGG ACGCGAACTG TGGCCCACCC
GACGGCTCCC AGCCAGCCGC CGCCGGCGCG GGCGCGTCTA AAGCTGGGAA CCCGGCTGCG
GCGGCGGCGG CGGCCGCGGC GGCAGTGGCG GCGGCGGCGG CGGCCGCAGC AGCCAAGCCC
TCGGACACCG GTGGCGGCGG CAGTGGAGGC GGCGCGGGGA GCCCCGGAGC GCAGGGCACC
AAATACCCGG AGCACGGCAA CCCGGCTATC CTACTTATGG GCTCAGCCAA CGGCGGGCCC
GTGGTCAAAA CTGACTCGCA GCAGCCTCTC GTATGGCCCG CCTGGGTGTA CTGCACACGT
TATTCGGATC GTCCATCCTC CGGTCCGCGC ACCAGGAAGC TGAAGAAGAA GAAGAACGAG
AAGGAGGACA AGCGGCCGCG GACCGCGTTC ACGGCCGAGC AGCTGCAGAG ACTCAAGGCG
GAGTTCCAGG CAAACCGCTA CATCACGGAG CAGCGGCGGC AGACCCTGGC CCAGGAACTC
AGCCTCAACG AGTCCCAGAT CAAGATCTGG TTCCAGAACA AGCGCGCCAA GATCAAGAAA
GCCACAGGCA TCAAGAACGG CCTGGCGCTG CACCTCATGG CCCAGGGACT GTACAACCAC
TCCACCACCA CGGTCCAGGA CAAAGACGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001417.3
CDS1017..2195
Misc Feature(1)897..899(+)
Misc Feature(2)1923..2102(+)
Misc Feature(3)1929..2096(+)
Misc Feature(4)1929..2096(+)
Misc Feature(5)1932..2084(+)
Misc Feature(6)2097..2192(+)
Exon (1)1..1878
Gene:EN1
Gene Synonym:
Exon (2)1879..2962
Gene:EN1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001426
Organism Homo sapiens (human)
Definition Homo sapiens engrailed homeobox 1 (EN1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001426

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAAGAAC AGCAGCCGGA ACCTAAAAGT CAGCGCGACT CGGCCCTCGG CGCGGCGGCG 
GCGGCGACTC CGGGCGGCCT CAGCCTGAGC CTCAGTCCGG GCGCCAGCGG CAGCAGCGGC
AGCGGCAGCG ATGGAGACAG CGTGCCGGTG TCCCCGCAGC CTGCGCCCCC CTCGCCGCCC
GCGGCGCCTT GCCTGCCGCC CCTGGCCCAC CACCCGCACC TCCCCCCACA CCCCCCGCCC
CCGCCGCCTC AGCATCTCGC GGCGCCTGCT CACCAGCCGC AGCCAGCGGC CCAGCTGCAC
CGCACCACCA ACTTTTTCAT CGACAACATC CTGAGGCCGG ACTTCGGCTG CAAAAAGGAG
CAGCCGCCAC CGCAGCTTCT GGTGGCTGCG GCGGCCAGAG GAGGCGCAGG AGGAGGAGGC
CGGGTCGAGC GTGACAGAGG CCAGACTGCC GCAGGTAGAG ACCCTGTCCA CCCGTTGGGC
ACCCGGGCGC CAGGCGCTGC CTCGCTCCTG TGCGCCCCGG ACGCGAACTG TGGCCCACCC
GACGGCTCCC AGCCAGCCGC CGCCGGCGCG GGCGCGTCTA AAGCTGGGAA CCCGGCTGCG
GCGGCGGCGG CGGCCGCGGC GGCAGTGGCG GCGGCGGCGG CGGCCGCAGC AGCCAAGCCC
TCGGACACCG GTGGCGGCGG CAGTGGAGGC GGCGCGGGGA GCCCCGGAGC GCAGGGCACC
AAATACCCGG AGCACGGCAA CCCGGCTATC CTACTTATGG GCTCAGCCAA CGGCGGGCCC
GTGGTCAAAA CTGACTCGCA GCAGCCTCTC GTATGGCCCG CCTGGGTGTA CTGCACACGT
TATTCGGATC GTCCATCCTC CGGTCCGCGC ACCAGGAAGC TGAAGAAGAA GAAGAACGAG
AAGGAGGACA AGCGGCCGCG GACCGCGTTC ACGGCCGAGC AGCTGCAGAG ACTCAAGGCG
GAGTTCCAGG CAAACCGCTA CATCACGGAG CAGCGGCGGC AGACCCTGGC CCAGGAACTC
AGCCTCAACG AGTCCCAGAT CAAGATCTGG TTCCAGAACA AGCGCGCCAA GATCAAGAAA
GCCACAGGCA TCAAGAACGG CCTGGCGCTG CACCTCATGG CCCAGGGACT GTACAACCAC
TCCACCACCA CGGTCCAGGA CAAAGACGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Developmental transcription factor EN1--a novel biomarker in human salivary gland adenoid cystic carcinoma
Cancer 118 (5), 1288-1292 (2012)
Bell D, Bell A, Roberts D, Weber RS and El-Naggar AK.


book

[The regulation of UTROPHIN expression by EN1]
Yi Chuan 33 (4), 347-352 (2011)
Wang Q, Cao DH, Lin CK, Wang ZD, Cui WT and Jin CL.


book

Replication of top markers of a genome-wide association study in multiple sclerosis in Spain
Genes Immun. 12 (2), 110-115 (2011)
Cavanillas ML, Fernandez O, Comabella M, Alcina A, Fedetz M, Izquierdo G, Lucas M, Cenit MC, Arroyo R, Vandenbroeck K, Alloza I, Garcia-Barcina M, Antiguedad A, Leyva L, Gomez CL, Olascoaga J, Otaegui D, Blanco Y, Saiz A, Montalban X, Matesanz F and Urcelay E.


book

A method of utrophin up-regulation through RNAi-mediated knockdown of the transcription factor EN1
J. Int. Med. Res. 39 (1), 161-171 (2011)
Wang Q, Cao DH, Jin CL, Lin CK, Ma HW and Wu YY.


book

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study
Diabetes Care 33 (10), 2250-2253 (2010)
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC and Anand S.


book

The mouse Engrailed-1 gene and ventral limb patterning
Nature 382 (6589), 360-363 (1996)
Loomis CA, Harris E, Michaud J, Wurst W, Hanks M and Joyner AL.


book

Deregulated expression of PAX5 in medulloblastoma
Proc. Natl. Acad. Sci. U.S.A. 92 (12), 5709-5713 (1995)
Kozmik Z, Sure U, Ruedi D, Busslinger M and Aguzzi A.


book

Regional assignment of the human homeobox-containing gene EN1 to chromosome 2q13-q21
Genomics 15 (1), 233-235 (1993)
Kohler A, Logan C, Joyner AL and Muenke M.


book

Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions
Dev. Genet. 13 (5), 345-358 (1992)
Logan C, Hanks MC, Noble-Topham S, Nallainathan D, Provart NJ and Joyner AL.


book

Chromosomal localization of the human homeo box-containing genes, EN1 and EN2
Genomics 4 (2), 206-209 (1989)
Logan C, Willard HF, Rommens JM and Joyner AL.


 
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