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EPHA3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EPHA3
Entrez Gene ID 2042
Full Name EPH receptor A3
Synonyms EK4, ETK, ETK1, HEK, HEK4, TYRO4
General protein information
Preferred Names
ephrin type-A receptor 3
Names
ephrin type-A receptor 3
EPH-like kinase 4
human embryo kinase 1
eph-like tyrosine kinase 1
TYRO4 protein tyrosine kinase
tyrosine-protein kinase receptor ETK1
NP_005224.2
NP_872585.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p11.2

Summary This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

179611


mRNA and Protein(s)

mRNA Protein Name
XM_005264715 XP_005264772 ephrin type-A receptor 3 isoform X1
XM_005264716 XP_005264773 ephrin type-A receptor 3 isoform X2
NM_182644 NP_872585 ephrin type-A receptor 3 isoform b precursor
NM_005233 NP_005224 ephrin type-A receptor 3 isoform a precursor



Homo sapiens (human) EPHA3 NP_005224.2
Pan troglodytes (chimpanzee) EPHA3 XP_516601.2
Macaca mulatta (Rhesus monkey) EPHA3 XP_001083136.1
Canis lupus familiaris (dog) EPHA3 XP_005639499.1
Bos taurus (cattle) EPHA3 NP_001193042.1
Mus musculus (house mouse) Epha3 NP_034270.1
Rattus norvegicus (Norway rat) Epha3 NP_113752.1
Gallus gallus (chicken) EPHA3 NP_990761.1
Xenopus (Silurana) tropicalis (western clawed frog) epha3 XP_002931819.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EPHA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu37037 XM_005264715 PREDICTED: Homo sapiens EPH receptor A3 (EPHA3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu37038 XM_005264716 PREDICTED: Homo sapiens EPH receptor A3 (EPHA3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu15678 NM_182644 Homo sapiens EPH receptor A3 (EPHA3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu19238 NM_005233 Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu37037
Clone ID Related Accession (Same CDS sequence) XM_005264715
Accession Version XM_005264715.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ephrin type-A receptor 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GGAACAAGAA
ACAAGTTATA CCATTCTGAG GGCAAGAGGC ACAAATGTTA CCATCAGTAG CCTCAAGCCT
GACACTATAT ACGTATTCCA AATCCGAGCC CGAACAGCCG CTGGATATGG GACGAACAGC
CGCAAGTTTG AGTTTGAAAC TAGTCCAGAC TCTTTCTCCA TCTCTGGTGA AAGTAGCCAA
GTGGTCATGA TCGCCATTTC AGCGGCAGTA GCAATTATTC TCCTCACTGT TGTCATCTAT
GTTTTGATTG GGAGGTTCTG TGGCTATAAG TCAAAACATG GGGCAGATGA AAAAAGACTT
CATTTTGGCA ATGGGCATTT AAAACTTCCA GGTCTCAGGA CTTATGTTGA CCCACATACA
TATGAAGACC CTACCCAAGC TGTTCATGAG TTTGCCAAGG AATTGGATGC CACCAACATA
TCCATTGATA AAGTTGTTGG AGCAGGTGAA TTTGGAGAGG TGTGCAGTGG TCGCTTAAAA
CTTCCTTCAA AAAAAGAGAT TTCAGTGGCC ATTAAGACCC TGAAAGTTGG CTACACAGAA
AAGCAGAGGA GAGACTTCCT GGGAGAAGCA AGCATTATGG GACAGTTTGA CCACCCCAAT
ATCATTCGAC TGGAAGGAGT TGTTACCAAA AGTAAGCCAG TTATGATTGT CACAGAATAC
ATGGAGAATG GTTCCTTGGA TAGTTTCCTA CGTAAACACG ATGCCCAGTT TACTGTCATT
CAGCTAGTGG GGATGCTTCG AGGGATAGCA TCTGGCATGA AGTACCTGTC AGACATGGGC
TATGTTCACC GAGACCTCGC TGCTCGGAAC ATCTTGATCA ACAGTAACTT GGTGTGTAAG
GTTTCTGATT TCGGACTTTC GCGTGTCCTG GAGGATGACC CAGAAGCTGC TTATACAACA
AGAGGAGGGA AGATCCCAAT CAGGTGGACA TCACCAGAAG CTATAGCCTA CCGCAAGTTC
ACGTCAGCCA GCGATGTATG GAGTTATGGG ATTGTTCTCT GGGAGGTGAT GTCTTATGGA
GAGAGACCAT ACTGGGAGAT GTCCAATCAG GATGTAATTA AAGCTGTAGA TGAGGGCTAT
CGACTGCCAC CCCCCATGGA CTGCCCAGCT GCCTTGTATC AGCTGATGCT GGACTGCTGG
CAGAAAGACA GGAACAACAG ACCCAAGTTT GAGCAGATTG TTAGTATTCT GGACAAGCTT
ATCCGGAATC CCGGCAGCCT GAAGATCATC ACCAGTGCAG CCGCAAGGCC ATCAAACCTT
CTTCTGGACC AAAGCAATGT GGATATCACT ACCTTCCGCA CAACAGGTGA CTGGCTTAAT
GGTGTCTGGA CAGCACACTG CAAGGAAATC TTCACGGGTG TGGAGTACAG TTCTTGTGAC
ACAATAGCCA AGATTTCCAC AGATGACATG AAAAAGGTTG GTGTCACCGT GGTTGGGCCA
CAGAAGAAGA TCATCAGTAG CATTAAAGCT CTAGAAACGC AATCAAAGAA TGGCCCAGTT
CCCGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264772.1
CDS129..3077
Misc Feature(1)213..731(+)
Misc Feature(2)285..707(+)
Misc Feature(3)1104..1424(+)
Misc Feature(4)1104..1370(+)
Misc Feature(5)1371..1385(+)
Misc Feature(6)1437..1709(+)
Misc Feature(7)1437..1673(+)
Misc Feature(8)1674..1688(+)
Misc Feature(9)1788..1979(+)
Misc Feature(10)1971..2771(+)
Misc Feature(11)1986..2759(+)
Misc Feature(12)2004..2615(+)
Misc Feature(13)2004..2417(+)
Misc Feature(14)2361..2615(+)
Misc Feature(15)2412..2492(+)
Misc Feature(16)2847..3050(+)
Misc Feature(17)2859..3047(+)
Translation

Target ORF information:

RefSeq Version XM_005264715
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EPH receptor A3 (EPHA3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264715

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GGAACAAGAA
ACAAGTTATA CCATTCTGAG GGCAAGAGGC ACAAATGTTA CCATCAGTAG CCTCAAGCCT
GACACTATAT ACGTATTCCA AATCCGAGCC CGAACAGCCG CTGGATATGG GACGAACAGC
CGCAAGTTTG AGTTTGAAAC TAGTCCAGAC TCTTTCTCCA TCTCTGGTGA AAGTAGCCAA
GTGGTCATGA TCGCCATTTC AGCGGCAGTA GCAATTATTC TCCTCACTGT TGTCATCTAT
GTTTTGATTG GGAGGTTCTG TGGCTATAAG TCAAAACATG GGGCAGATGA AAAAAGACTT
CATTTTGGCA ATGGGCATTT AAAACTTCCA GGTCTCAGGA CTTATGTTGA CCCACATACA
TATGAAGACC CTACCCAAGC TGTTCATGAG TTTGCCAAGG AATTGGATGC CACCAACATA
TCCATTGATA AAGTTGTTGG AGCAGGTGAA TTTGGAGAGG TGTGCAGTGG TCGCTTAAAA
CTTCCTTCAA AAAAAGAGAT TTCAGTGGCC ATTAAGACCC TGAAAGTTGG CTACACAGAA
AAGCAGAGGA GAGACTTCCT GGGAGAAGCA AGCATTATGG GACAGTTTGA CCACCCCAAT
ATCATTCGAC TGGAAGGAGT TGTTACCAAA AGTAAGCCAG TTATGATTGT CACAGAATAC
ATGGAGAATG GTTCCTTGGA TAGTTTCCTA CGTAAACACG ATGCCCAGTT TACTGTCATT
CAGCTAGTGG GGATGCTTCG AGGGATAGCA TCTGGCATGA AGTACCTGTC AGACATGGGC
TATGTTCACC GAGACCTCGC TGCTCGGAAC ATCTTGATCA ACAGTAACTT GGTGTGTAAG
GTTTCTGATT TCGGACTTTC GCGTGTCCTG GAGGATGACC CAGAAGCTGC TTATACAACA
AGAGGAGGGA AGATCCCAAT CAGGTGGACA TCACCAGAAG CTATAGCCTA CCGCAAGTTC
ACGTCAGCCA GCGATGTATG GAGTTATGGG ATTGTTCTCT GGGAGGTGAT GTCTTATGGA
GAGAGACCAT ACTGGGAGAT GTCCAATCAG GATGTAATTA AAGCTGTAGA TGAGGGCTAT
CGACTGCCAC CCCCCATGGA CTGCCCAGCT GCCTTGTATC AGCTGATGCT GGACTGCTGG
CAGAAAGACA GGAACAACAG ACCCAAGTTT GAGCAGATTG TTAGTATTCT GGACAAGCTT
ATCCGGAATC CCGGCAGCCT GAAGATCATC ACCAGTGCAG CCGCAAGGCC ATCAAACCTT
CTTCTGGACC AAAGCAATGT GGATATCACT ACCTTCCGCA CAACAGGTGA CTGGCTTAAT
GGTGTCTGGA CAGCACACTG CAAGGAAATC TTCACGGGTG TGGAGTACAG TTCTTGTGAC
ACAATAGCCA AGATTTCCAC AGATGACATG AAAAAGGTTG GTGTCACCGT GGTTGGGCCA
CAGAAGAAGA TCATCAGTAG CATTAAAGCT CTAGAAACGC AATCAAAGAA TGGCCCAGTT
CCCGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37038
Clone ID Related Accession (Same CDS sequence) XM_005264716
Accession Version XM_005264716.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ephrin type-A receptor 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA
GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG
CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC
AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTCTTTCT CCATCTCTGG TGAAAGTAGC
CAAGTGGTCA TGATCGCCAT TTCAGCGGCA GTAGCAATTA TTCTCCTCAC TGTTGTCATC
TATGTTTTGA TTGGGAGGTT CTGTGGCTAT AAGTCAAAAC ATGGGGCAGA TGAAAAAAGA
CTTCATTTTG GCAATGGGCA TTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAT
ACATATGAAG ACCCTACCCA AGCTGTTCAT GAGTTTGCCA AGGAATTGGA TGCCACCAAC
ATATCCATTG ATAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGTCGCTTA
AAACTTCCTT CAAAAAAAGA GATTTCAGTG GCCATTAAGA CCCTGAAAGT TGGCTACACA
GAAAAGCAGA GGAGAGACTT CCTGGGAGAA GCAAGCATTA TGGGACAGTT TGACCACCCC
AATATCATTC GACTGGAAGG AGTTGTTACC AAAAGTAAGC CAGTTATGAT TGTCACAGAA
TACATGGAGA ATGGTTCCTT GGATAGTTTC CTACGTAAAC ACGATGCCCA GTTTACTGTC
ATTCAGCTAG TGGGGATGCT TCGAGGGATA GCATCTGGCA TGAAGTACCT GTCAGACATG
GGCTATGTTC ACCGAGACCT CGCTGCTCGG AACATCTTGA TCAACAGTAA CTTGGTGTGT
AAGGTTTCTG ATTTCGGACT TTCGCGTGTC CTGGAGGATG ACCCAGAAGC TGCTTATACA
ACAAGAGGAG GGAAGATCCC AATCAGGTGG ACATCACCAG AAGCTATAGC CTACCGCAAG
TTCACGTCAG CCAGCGATGT ATGGAGTTAT GGGATTGTTC TCTGGGAGGT GATGTCTTAT
GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT AGATGAGGGC
TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCCTTGT ATCAGCTGAT GCTGGACTGC
TGGCAGAAAG ACAGGAACAA CAGACCCAAG TTTGAGCAGA TTGTTAGTAT TCTGGACAAG
CTTATCCGGA ATCCCGGCAG CCTGAAGATC ATCACCAGTG CAGCCGCAAG GCCATCAAAC
CTTCTTCTGG ACCAAAGCAA TGTGGATATC ACTACCTTCC GCACAACAGT GACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264773.1
CDS129..2885
Misc Feature(1)213..731(+)
Misc Feature(2)285..707(+)
Misc Feature(3)1104..1424(+)
Misc Feature(4)1104..1370(+)
Misc Feature(5)1371..1385(+)
Misc Feature(6)1437..1712(+)
Misc Feature(7)1437..1676(+)
Misc Feature(8)1677..1691(+)
Misc Feature(9)1791..1982(+)
Misc Feature(10)1974..2774(+)
Misc Feature(11)1989..2762(+)
Misc Feature(12)2007..2618(+)
Misc Feature(13)2007..2420(+)
Misc Feature(14)2364..2618(+)
Misc Feature(15)2415..2495(+)
Translation

Target ORF information:

RefSeq Version XM_005264716
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EPH receptor A3 (EPHA3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264716

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA
GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG
CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC
AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTCTTTCT CCATCTCTGG TGAAAGTAGC
CAAGTGGTCA TGATCGCCAT TTCAGCGGCA GTAGCAATTA TTCTCCTCAC TGTTGTCATC
TATGTTTTGA TTGGGAGGTT CTGTGGCTAT AAGTCAAAAC ATGGGGCAGA TGAAAAAAGA
CTTCATTTTG GCAATGGGCA TTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAT
ACATATGAAG ACCCTACCCA AGCTGTTCAT GAGTTTGCCA AGGAATTGGA TGCCACCAAC
ATATCCATTG ATAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGTCGCTTA
AAACTTCCTT CAAAAAAAGA GATTTCAGTG GCCATTAAGA CCCTGAAAGT TGGCTACACA
GAAAAGCAGA GGAGAGACTT CCTGGGAGAA GCAAGCATTA TGGGACAGTT TGACCACCCC
AATATCATTC GACTGGAAGG AGTTGTTACC AAAAGTAAGC CAGTTATGAT TGTCACAGAA
TACATGGAGA ATGGTTCCTT GGATAGTTTC CTACGTAAAC ACGATGCCCA GTTTACTGTC
ATTCAGCTAG TGGGGATGCT TCGAGGGATA GCATCTGGCA TGAAGTACCT GTCAGACATG
GGCTATGTTC ACCGAGACCT CGCTGCTCGG AACATCTTGA TCAACAGTAA CTTGGTGTGT
AAGGTTTCTG ATTTCGGACT TTCGCGTGTC CTGGAGGATG ACCCAGAAGC TGCTTATACA
ACAAGAGGAG GGAAGATCCC AATCAGGTGG ACATCACCAG AAGCTATAGC CTACCGCAAG
TTCACGTCAG CCAGCGATGT ATGGAGTTAT GGGATTGTTC TCTGGGAGGT GATGTCTTAT
GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT AGATGAGGGC
TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCCTTGT ATCAGCTGAT GCTGGACTGC
TGGCAGAAAG ACAGGAACAA CAGACCCAAG TTTGAGCAGA TTGTTAGTAT TCTGGACAAG
CTTATCCGGA ATCCCGGCAG CCTGAAGATC ATCACCAGTG CAGCCGCAAG GCCATCAAAC
CTTCTTCTGG ACCAAAGCAA TGTGGATATC ACTACCTTCC GCACAACAGT GACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15678
Clone ID Related Accession (Same CDS sequence) NM_182644
Accession Version NM_182644.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ephrin type-A receptor 3 isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF213459.1 and AC109129.3. On Aug 28, 2007 this sequence version replaced gi:32967313. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in missing several 3' exons. It encodes isoform b which has a shorter and distinct C-terminus compared to isoform a. This isoform lacks a transmembrane domain and may be a secreted form of the Epha3 receptor. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF213460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA
GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG
CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC
AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTGTATGT ATTATTTCAA TGCAGTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_872585.1
CDS226..1845
Misc Feature(1)136..138(+)
Misc Feature(2)310..828(+)
Misc Feature(3)382..804(+)
Misc Feature(4)1033..1128(+)
Misc Feature(5)1201..1521(+)
Misc Feature(6)1201..1467(+)
Misc Feature(7)1468..1482(+)
Misc Feature(8)1534..1809(+)
Misc Feature(9)1534..1773(+)
Misc Feature(10)1774..1788(+)
Exon (1)1..313
Gene:EPHA3
Gene Synonym:
Exon (2)314..378
Gene:EPHA3
Gene Synonym:
Exon (3)379..1039
Gene:EPHA3
Gene Synonym:
Exon (4)1040..1195
Gene:EPHA3
Gene Synonym:
Exon (5)1196..1531
Gene:EPHA3
Gene Synonym:
Exon (6)1532..1656
Gene:EPHA3
Gene Synonym:
Exon (7)1657..2684
Gene:EPHA3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_182644
Organism Homo sapiens (human)
Definition Homo sapiens EPH receptor A3 (EPHA3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_182644

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA
GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG
CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC
AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTGTATGT ATTATTTCAA TGCAGTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19238
Clone ID Related Accession (Same CDS sequence) NM_005233
Accession Version NM_005233.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ephrin type-A receptor 3 isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF213459.1 and BC063282.1. This sequence is a reference standard in the RefSeqGene project. On Aug 28, 2007 this sequence version replaced gi:156071498. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF213459.1, BC063282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA
GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG
CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC
AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTCTTTCT CCATCTCTGG TGAAAGTAGC
CAAGTGGTCA TGATCGCCAT TTCAGCGGCA GTAGCAATTA TTCTCCTCAC TGTTGTCATC
TATGTTTTGA TTGGGAGGTT CTGTGGCTAT AAGTCAAAAC ATGGGGCAGA TGAAAAAAGA
CTTCATTTTG GCAATGGGCA TTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAT
ACATATGAAG ACCCTACCCA AGCTGTTCAT GAGTTTGCCA AGGAATTGGA TGCCACCAAC
ATATCCATTG ATAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGTCGCTTA
AAACTTCCTT CAAAAAAAGA GATTTCAGTG GCCATTAAGA CCCTGAAAGT TGGCTACACA
GAAAAGCAGA GGAGAGACTT CCTGGGAGAA GCAAGCATTA TGGGACAGTT TGACCACCCC
AATATCATTC GACTGGAAGG AGTTGTTACC AAAAGTAAGC CAGTTATGAT TGTCACAGAA
TACATGGAGA ATGGTTCCTT GGATAGTTTC CTACGTAAAC ACGATGCCCA GTTTACTGTC
ATTCAGCTAG TGGGGATGCT TCGAGGGATA GCATCTGGCA TGAAGTACCT GTCAGACATG
GGCTATGTTC ACCGAGACCT CGCTGCTCGG AACATCTTGA TCAACAGTAA CTTGGTGTGT
AAGGTTTCTG ATTTCGGACT TTCGCGTGTC CTGGAGGATG ACCCAGAAGC TGCTTATACA
ACAAGAGGAG GGAAGATCCC AATCAGGTGG ACATCACCAG AAGCTATAGC CTACCGCAAG
TTCACGTCAG CCAGCGATGT ATGGAGTTAT GGGATTGTTC TCTGGGAGGT GATGTCTTAT
GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT AGATGAGGGC
TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCCTTGT ATCAGCTGAT GCTGGACTGC
TGGCAGAAAG ACAGGAACAA CAGACCCAAG TTTGAGCAGA TTGTTAGTAT TCTGGACAAG
CTTATCCGGA ATCCCGGCAG CCTGAAGATC ATCACCAGTG CAGCCGCAAG GCCATCAAAC
CTTCTTCTGG ACCAAAGCAA TGTGGATATC ACTACCTTCC GCACAACAGG TGACTGGCTT
AATGGTGTCT GGACAGCACA CTGCAAGGAA ATCTTCACGG GTGTGGAGTA CAGTTCTTGT
GACACAATAG CCAAGATTTC CACAGATGAC ATGAAAAAGG TTGGTGTCAC CGTGGTTGGG
CCACAGAAGA AGATCATCAG TAGCATTAAA GCTCTAGAAA CGCAATCAAA GAATGGCCCA
GTTCCCGTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005224.2
CDS226..3177
Misc Feature(1)136..138(+)
Misc Feature(2)310..828(+)
Misc Feature(3)382..804(+)
Misc Feature(4)1201..1521(+)
Misc Feature(5)1201..1467(+)
Misc Feature(6)1468..1482(+)
Misc Feature(7)1534..1809(+)
Misc Feature(8)1534..1773(+)
Misc Feature(9)1774..1788(+)
Misc Feature(10)1849..1920(+)
Misc Feature(11)1888..2079(+)
Misc Feature(12)2011..2013(+)
Misc Feature(13)2011..2013(+)
Misc Feature(14)2029..2031(+)
Misc Feature(15)2029..2031(+)
Misc Feature(16)2071..2871(+)
Misc Feature(17)2086..2859(+)
Misc Feature(18)2104..2715(+)
Misc Feature(19)2104..2517(+)
Misc Feature(20)2326..2328(+)
Misc Feature(21)2461..2715(+)
Misc Feature(22)2512..2592(+)
Misc Feature(23)2560..2562(+)
Misc Feature(24)2560..2562(+)
Misc Feature(25)2947..3150(+)
Misc Feature(26)2959..3147(+)
Misc Feature(27)3166..3174(+)
Exon (1)1..313
Gene:EPHA3
Gene Synonym:
Exon (2)314..378
Gene:EPHA3
Gene Synonym:
Exon (3)379..1039
Gene:EPHA3
Gene Synonym:
Exon (4)1040..1195
Gene:EPHA3
Gene Synonym:
Exon (5)1196..1531
Gene:EPHA3
Gene Synonym:
Exon (6)1532..1656
Gene:EPHA3
Gene Synonym:
Exon (7)1657..1819
Gene:EPHA3
Gene Synonym:
Exon (8)1820..1922
Gene:EPHA3
Gene Synonym:
Exon (9)1923..1987
Gene:EPHA3
Gene Synonym:
Exon (10)1988..2113
Gene:EPHA3
Gene Synonym:
Exon (11)2114..2299
Gene:EPHA3
Gene Synonym:
Exon (12)2300..2361
Gene:EPHA3
Gene Synonym:
Exon (13)2362..2571
Gene:EPHA3
Gene Synonym:
Exon (14)2572..2721
Gene:EPHA3
Gene Synonym:
Exon (15)2722..2915
Gene:EPHA3
Gene Synonym:
Exon (16)2916..3071
Gene:EPHA3
Gene Synonym:
Exon (17)3072..5809
Gene:EPHA3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005233
Organism Homo sapiens (human)
Definition Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005233

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGATTGTC AGCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 
GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG
GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA
CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT
TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG
TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC
AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT
ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT
TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA
AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC
CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA
AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT
GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA
TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT
TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG
GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC
ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC
CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG
GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC
CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC
CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT
GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA
GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG
CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC
AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTCTTTCT CCATCTCTGG TGAAAGTAGC
CAAGTGGTCA TGATCGCCAT TTCAGCGGCA GTAGCAATTA TTCTCCTCAC TGTTGTCATC
TATGTTTTGA TTGGGAGGTT CTGTGGCTAT AAGTCAAAAC ATGGGGCAGA TGAAAAAAGA
CTTCATTTTG GCAATGGGCA TTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAT
ACATATGAAG ACCCTACCCA AGCTGTTCAT GAGTTTGCCA AGGAATTGGA TGCCACCAAC
ATATCCATTG ATAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGTCGCTTA
AAACTTCCTT CAAAAAAAGA GATTTCAGTG GCCATTAAGA CCCTGAAAGT TGGCTACACA
GAAAAGCAGA GGAGAGACTT CCTGGGAGAA GCAAGCATTA TGGGACAGTT TGACCACCCC
AATATCATTC GACTGGAAGG AGTTGTTACC AAAAGTAAGC CAGTTATGAT TGTCACAGAA
TACATGGAGA ATGGTTCCTT GGATAGTTTC CTACGTAAAC ACGATGCCCA GTTTACTGTC
ATTCAGCTAG TGGGGATGCT TCGAGGGATA GCATCTGGCA TGAAGTACCT GTCAGACATG
GGCTATGTTC ACCGAGACCT CGCTGCTCGG AACATCTTGA TCAACAGTAA CTTGGTGTGT
AAGGTTTCTG ATTTCGGACT TTCGCGTGTC CTGGAGGATG ACCCAGAAGC TGCTTATACA
ACAAGAGGAG GGAAGATCCC AATCAGGTGG ACATCACCAG AAGCTATAGC CTACCGCAAG
TTCACGTCAG CCAGCGATGT ATGGAGTTAT GGGATTGTTC TCTGGGAGGT GATGTCTTAT
GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT AGATGAGGGC
TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCCTTGT ATCAGCTGAT GCTGGACTGC
TGGCAGAAAG ACAGGAACAA CAGACCCAAG TTTGAGCAGA TTGTTAGTAT TCTGGACAAG
CTTATCCGGA ATCCCGGCAG CCTGAAGATC ATCACCAGTG CAGCCGCAAG GCCATCAAAC
CTTCTTCTGG ACCAAAGCAA TGTGGATATC ACTACCTTCC GCACAACAGG TGACTGGCTT
AATGGTGTCT GGACAGCACA CTGCAAGGAA ATCTTCACGG GTGTGGAGTA CAGTTCTTGT
GACACAATAG CCAAGATTTC CACAGATGAC ATGAAAAAGG TTGGTGTCAC CGTGGTTGGG
CCACAGAAGA AGATCATCAG TAGCATTAAA GCTCTAGAAA CGCAATCAAA GAATGGCCCA
GTTCCCGTGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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