×

EPO cDNA ORF clone, Homo sapiens (human)

Gene Symbol EPO
Entrez Gene ID 2056
Full Name erythropoietin
Synonyms EP, MVCD2
General protein information
Preferred Names
erythropoietin
Names
erythropoietin
epoetin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q22

Summary This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

133170

Disorder Html: {Microvascular complications of diabetes 2}, 612623 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000799 NP_000790 erythropoietin precursor



Homo sapiens (human) EPO NP_000790.2
Pan troglodytes (chimpanzee) EPO XP_519268.2
Macaca mulatta (Rhesus monkey) EPO NP_001036201.1
Canis lupus familiaris (dog) EPO NP_001006647.1
Bos taurus (cattle) EPO NP_776334.1
Mus musculus (house mouse) Epo NP_031968.1
Rattus norvegicus (Norway rat) Epo NP_058697.1
Danio rerio (zebrafish) epo NP_001033098.1
Danio rerio (zebrafish) LOC100002479 XP_001342254.1
Xenopus (Silurana) tropicalis (western clawed frog) epo NP_001233194.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20340
NM_000799 Homo sapiens erythropoietin (EPO), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20340
Clone ID Related Accession (Same CDS sequence) NM_000799
Accession Version NM_000799.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product erythropoietin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02157.1 and S65458.1. This sequence is a reference standard in the RefSeqGene project. On Apr 6, 2005 this sequence version replaced gi:4503588. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02157.1, BC093628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2159368 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCCCTGCT GTCGCTCCCT 
CTGGGCCTCC CAGTCCTGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATATCACGA CGGGCTGTGC TGAACACTGC
AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
ATGGAGGTCG GGCAGCAGGC CGTAGAAGTC TGGCAGGGCC TGGCCCTGCT GTCGGAAGCT
GTCCTGCGGG GCCAGGCCCT GTTGGTCAAC TCTTCCCAGC CGTGGGAGCC CCTGCAGCTG
CATGTGGATA AAGCCGTCAG TGGCCTTCGC AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
GCCCAGAAGG AAGCCATCTC CCCTCCAGAT GCGGCCTCAG CTGCTCCACT CCGAACAATC
ACTGCTGACA CTTTCCGCAA ACTCTTCCGA GTCTACTCCA ATTTCCTCCG GGGAAAGCTG
AAGCTGTACA CAGGGGAGGC CTGCAGGACA GGGGACAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000790.2
CDS182..763
Misc Feature(1)116..118(+)
Misc Feature(2)269..760(+)
Misc Feature(3)281..745(+)
Misc Feature(4)332..334(+)
Misc Feature(5)347..361(+)
Misc Feature(6)374..376(+)
Misc Feature(7)509..511(+)
Misc Feature(8)638..640(+)
Exon (1)1..194
Gene:EPO
Gene Synonym:
Exon (2)195..340
Gene:EPO
Gene Synonym:
Exon (3)341..427
Gene:EPO
Gene Synonym:
Exon (4)428..607
Gene:EPO
Gene Synonym:
Exon (5)608..1330
Gene:EPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000799
Organism Homo sapiens (human)
Definition Homo sapiens erythropoietin (EPO), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000799

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCCCTGCT GTCGCTCCCT 
CTGGGCCTCC CAGTCCTGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATATCACGA CGGGCTGTGC TGAACACTGC
AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
ATGGAGGTCG GGCAGCAGGC CGTAGAAGTC TGGCAGGGCC TGGCCCTGCT GTCGGAAGCT
GTCCTGCGGG GCCAGGCCCT GTTGGTCAAC TCTTCCCAGC CGTGGGAGCC CCTGCAGCTG
CATGTGGATA AAGCCGTCAG TGGCCTTCGC AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
GCCCAGAAGG AAGCCATCTC CCCTCCAGAT GCGGCCTCAG CTGCTCCACT CCGAACAATC
ACTGCTGACA CTTTCCGCAA ACTCTTCCGA GTCTACTCCA ATTTCCTCCG GGGAAAGCTG
AAGCTGTACA CAGGGGAGGC CTGCAGGACA GGGGACAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Paraneoplastic hormones: parathyroid hormone-related protein (PTHrP) and erythropoietin (EPO) are related to vascular endothelial growth factor (VEGF) expression in clear cell renal cell carcinoma
Tumour Biol. 34 (6), 3471-3476 (2013)
Feng CC, Ding GX, Song NH, Li X, Wu Z, Jiang HW and Ding Q.


book

Erythropoietin is involved in the angiogenic potential of bone marrow macrophages in multiple myeloma
Angiogenesis 16 (4), 963-973 (2013)
De Luisi A, Binetti L, Ria R, Ruggieri S, Berardi S, Catacchio I, Racanelli V, Pavone V, Rossini B, Vacca A and Ribatti D.


book

Asymmetric dimethylarginine in response to recombinant tissue-type plasminogen activator and erythropoietin in acute stroke
Stroke 44 (8), 2128-2133 (2013)
Worthmann H, Martens-Lobenhoffer J, Joumaah M, Li N, Lichtinghagen R, Hecker H, Kielstein JT, Ehrenreich H, Bode-Boger SM and Weissenborn K.


book

The role and regulation of secretion of erythropoietin in pregnancy
Med Wieku Rozwoj 17 (3), 270-275 (2013)
Kowalska-Kanka A, Maciejewski T and Niemiec KT.


book

Urine erythropoietin level is associated with kidney and brain injury in critically ill neonates
Neonatology 104 (2), 87-94 (2013)
Li Y, Xiao Z, Yan J, Li X, Wang Q, Zhu H, Pan J, Zhu X, Wang J and Feng X.


book

Structures and functional roles of the sugar chains of human erythropoietins
Glycobiology 1 (4), 337-346 (1991)
Takeuchi M and Kobata A.


book

Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein
Science 242 (4884), 1412-1415 (1988)
Goldberg MA, Dunning SP and Bunn HF.


book

Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry
Biochemistry 27 (23), 8618-8626 (1988)
Sasaki H, Ochi N, Dell A and Fukuda M.


book

Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese hamster ovary cells
J. Biol. Chem. 263 (8), 3657-3663 (1988)
Takeuchi M, Takasaki S, Miyazaki H, Kato T, Hoshi S, Kochibe N and Kobata A.


book

Regional assignment of the erythropoietin gene to human chromosome region 7pter----q22
Cytogenet. Cell Genet. 42 (4), 214-218 (1986)
Watkins,P.C., Eddy,R., Hoffman,N., Stanislovitis,P., Beck,A.K., Galli,J., Vellucci,V., Gusella,J.F. and Shows,T.B.


 
*
*
*
*