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EPO cDNA ORF clone, Homo sapiens (human)

Gene Symbol EPO
Entrez Gene ID 2056
Full Name erythropoietin
Synonyms EP, MVCD2
General protein information
Preferred Names
erythropoietin
Names
erythropoietin
epoetin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q22

Summary This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

133170

Disorder Html: {Microvascular complications of diabetes 2}, 612623 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000799 NP_000790 erythropoietin precursor

KEGG
hsa04060 Cytokine-cytokine receptor interaction
hsa04630 Jak-STAT signaling pathway
hsa04640 Hematopoietic cell lineage
hsa04151 PI3K-Akt signaling pathway
hsa04066 HIF-1 signaling pathway
Reactome
R-HSA-2262752 Cellular responses to stress
R-HSA-2262749 Cellular response to hypoxia
R-HSA-1234174 Regulation of Hypoxia-inducible Factor (HIF) by oxygen
R-HSA-1234158 Regulation of gene expression by Hypoxia-inducible Factor
Pathway Interaction Database
hif2pathway HIF-2-alpha transcription factor network
epopathway EPO signaling pathway
hif1_tfpathway HIF-1-alpha transcription factor network
kitpathway Signaling events mediated by Stem cell factor receptor (c-Kit)
a4b1_paxindep_pathway Paxillin-independent events mediated by a4b1 and a4b7
WikiPathways
WP581 EPO Receptor Signaling


Homo sapiens (human) EPO NP_000790.2
Pan troglodytes (chimpanzee) EPO XP_519268.2
Macaca mulatta (Rhesus monkey) EPO NP_001036201.1
Canis lupus familiaris (dog) EPO NP_001006647.1
Bos taurus (cattle) EPO NP_776334.1
Mus musculus (house mouse) Epo NP_031968.1
Rattus norvegicus (Norway rat) Epo NP_058697.1
Danio rerio (zebrafish) epo NP_001033098.1
Danio rerio (zebrafish) LOC100002479 XP_001342254.1
Xenopus (Silurana) tropicalis (western clawed frog) epo NP_001233194.1

Component

ID Name Evidence
GO:0005576 extracellular region IEA
GO:0005615 extracellular space IDA

Function

ID Name Evidence
GO:0005128 erythropoietin receptor binding IEA
GO:0005179 hormone activity IEA
GO:0005515 protein binding IPI
GO:0043499 eukaryotic cell surface binding IDA

Process

ID Name Evidence
GO:0001666 response to hypoxia IEA
GO:0006357 regulation of transcription from RNA polymerase II promoter IEA
GO:0007165 signal transduction NAS
GO:0007566 embryo implantation IEA
GO:0007568 aging IEA
GO:0007584 response to nutrient IEA
GO:0008015 blood circulation NAS
GO:0008284 positive regulation of cell proliferation IDA
GO:0009651 response to salt stress IEA
GO:0010766 negative regulation of sodium ion transport IDA
GO:0030218 erythrocyte differentiation IEA
GO:0032413 negative regulation of ion transmembrane transporter activity IDA
GO:0032496 response to lipopolysaccharide IEA
GO:0033189 response to vitamin A IEA
GO:0033574 response to testosterone stimulus IEA
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein IDA
GO:0042541 hemoglobin biosynthetic process IEA
GO:0043066 negative regulation of apoptosis IDA
GO:0043249 erythrocyte maturation IEA
GO:0043526 neuroprotection IEA
GO:0043627 response to estrogen stimulus IEA
GO:0045666 positive regulation of neuron differentiation IEA
GO:0045740 positive regulation of DNA replication IDA
GO:0045893 positive regulation of transcription, DNA-dependent IDA
GO:0046579 positive regulation of Ras protein signal transduction IDA
GO:0048678 response to axon injury IEA
GO:0051602 response to electrical stimulus IEA
GO:0055093 response to hyperoxia IEA
GO:0070555 response to interleukin-1 IEA
GO:0071474 cellular hyperosmotic response IDA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20340
NM_000799 Homo sapiens erythropoietin (EPO), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu20340
Accession Version NM_000799.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product erythropoietin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02157.1 and S65458.1. This sequence is a reference standard in the RefSeqGene project. On Apr 6, 2005 this sequence version replaced gi:4503588. Summary: This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02157.1, BC093628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2159368 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCCCTGCT GTCGCTCCCT 
CTGGGCCTCC CAGTCCTGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATATCACGA CGGGCTGTGC TGAACACTGC
AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
ATGGAGGTCG GGCAGCAGGC CGTAGAAGTC TGGCAGGGCC TGGCCCTGCT GTCGGAAGCT
GTCCTGCGGG GCCAGGCCCT GTTGGTCAAC TCTTCCCAGC CGTGGGAGCC CCTGCAGCTG
CATGTGGATA AAGCCGTCAG TGGCCTTCGC AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
GCCCAGAAGG AAGCCATCTC CCCTCCAGAT GCGGCCTCAG CTGCTCCACT CCGAACAATC
ACTGCTGACA CTTTCCGCAA ACTCTTCCGA GTCTACTCCA ATTTCCTCCG GGGAAAGCTG
AAGCTGTACA CAGGGGAGGC CTGCAGGACA GGGGACAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000790.2
CDS182..763
Misc Feature(1)116..118(+)
Misc Feature(2)269..760(+)
Misc Feature(3)281..745(+)
Misc Feature(4)332..334(+)
Misc Feature(5)347..361(+)
Misc Feature(6)374..376(+)
Misc Feature(7)509..511(+)
Misc Feature(8)638..640(+)
Exon (1)1..194
Gene:EPO
Gene Synonym:
Exon (2)195..340
Gene:EPO
Gene Synonym:
Exon (3)341..427
Gene:EPO
Gene Synonym:
Exon (4)428..607
Gene:EPO
Gene Synonym:
Exon (5)608..1330
Gene:EPO
Gene Synonym:
Translation
Position Chain Variation Link
10 10 c, g dbSNP:376713532
14 14 c, t dbSNP:761765700
127 127 -, a dbSNP:34144627
127 127 g, t dbSNP:746847015
136 136 a, g dbSNP:756641457
140 140 a, g dbSNP:777893099
142 142 c, t dbSNP:141701177
146 146 a, c, t dbSNP:757521071
152 152 g, t dbSNP:745609348
162 162 a, g dbSNP:771913982
165 165 c, t dbSNP:779789360
170 170 c, t dbSNP:746962384
173 173 g, t dbSNP:768536667
174 174 c, g dbSNP:776523447
187 187 a, g dbSNP:776099080
190 190 a, g dbSNP:759518255
200 200 c, t dbSNP:747612452
218 218 c, t dbSNP:77762674
220 220 c, t dbSNP:541174516
226 226 a, c, t dbSNP:767873303
234 234 c, t dbSNP:770536039
235 235 a, g dbSNP:73409072
237 237 g, t dbSNP:760976625
257 257 c, g, t dbSNP:764644750
262 262 c, t dbSNP:750729016
268 268 a, c dbSNP:761916506
271 271 a, c dbSNP:765404850
272 272 c, t dbSNP:750589543
289 289 c, t dbSNP:140045752
291 291 a, g dbSNP:766552442
293 293 a, g dbSNP:200020971
302 302 a, c dbSNP:780420565
311 311 c, t dbSNP:754755075
326 326 g, t dbSNP:781053357
327 327 c, t dbSNP:748086642
328 328 c, g, t dbSNP:149877548
329 329 a, g dbSNP:748786072
335 335 a, t dbSNP:770575141
339 339 c, t dbSNP:773895305
340 340 c, g dbSNP:747340501
342 342 c, t dbSNP:201069425
344 344 c, g dbSNP:778559798
352 352 c, t dbSNP:61735970
356 356 c, t dbSNP:369033773
370 370 c, t dbSNP:781673396
382 382 c, t dbSNP:748564118
389 389 a, g dbSNP:62483572
396 396 a, g dbSNP:570715685
409 409 -, t dbSNP:34937405
409 409 g, t dbSNP:763035217
418 418 a, g dbSNP:57694744
422 422 a, g dbSNP:774561885
430 430 c, t dbSNP:749758292
431 431 a, c, g dbSNP:137953994
433 433 a, g dbSNP:759640016
442 442 c, t dbSNP:140683829
443 443 g, t dbSNP:370493713
450 450 c, t dbSNP:760394172
452 452 -, g dbSNP:71517124
464 464 a, g dbSNP:763792025
465 465 a, c dbSNP:776547281
467 467 c, g dbSNP:761633990
474 474 c, t dbSNP:765261245
475 475 a, g dbSNP:374875798
481 481 c, t dbSNP:758007491
488 488 c, t dbSNP:367645892
490 490 c, g dbSNP:751264643
491 491 a, g dbSNP:754729905
492 492 a, g, t dbSNP:777990553
497 497 a, g dbSNP:757662591
498 498 a, c dbSNP:779493836
505 505 a, g dbSNP:745887893
506 506 a, g dbSNP:772142028
517 517 c, t dbSNP:569327723
519 519 a, c dbSNP:775612706
522 522 c, t dbSNP:11976235
523 523 a, g dbSNP:768971818
529 529 a, g dbSNP:776259969
533 533 c, t dbSNP:761700728
540 540 c, t dbSNP:551748179
548 548 c, g dbSNP:773297902
552 552 a, g dbSNP:762488723
556 556 c, t dbSNP:566780061
557 557 a, g dbSNP:372093552
564 564 c, g dbSNP:746684403
570 570 a, g dbSNP:200241729
573 573 c, g dbSNP:376551627
580 580 c, t dbSNP:754099124
590 590 c, t dbSNP:369003114
591 591 a, g dbSNP:376745626
592 592 a, g dbSNP:746367858
599 599 c, g dbSNP:1126887
602 602 c, g dbSNP:758961204
611 611 a, g dbSNP:201051019
617 617 a, c dbSNP:780560676
619 619 c, t dbSNP:751552242
620 620 c, t dbSNP:755088347
621 621 c, g dbSNP:149431976
624 624 c, t dbSNP:748386526
625 625 g, t dbSNP:375550656
629 629 c, g dbSNP:770204024
633 633 a, c, t dbSNP:370232752
634 634 a, g dbSNP:78925197
640 640 a, g dbSNP:774438389
641 641 a, c, g dbSNP:758914998
648 648 c, t dbSNP:373734290
650 650 c, g dbSNP:760345451
652 652 c, t dbSNP:763785260
654 654 a, g dbSNP:750737268
664 664 c, t dbSNP:763338387
668 668 c, g dbSNP:201326665
673 673 g, t dbSNP:752118071
677 677 c, t dbSNP:73409075
678 678 a, g dbSNP:781324278
689 689 c, t dbSNP:752755372
690 690 a, g dbSNP:756377367
702 702 a, g dbSNP:778175956
721 721 a, g dbSNP:749028009
728 728 c, t dbSNP:757134843
732 732 c, t dbSNP:778634537
733 733 a, c, g dbSNP:144031260
740 740 g, t dbSNP:368744075
747 747 a, g dbSNP:746545386
750 750 c, t dbSNP:768257400
754 754 a, g dbSNP:776269617
755 755 a, g dbSNP:763243146
756 756 a, g dbSNP:750759945
757 757 c, g, t dbSNP:530329727
758 758 -, a dbSNP:369859204
758 758 a, g dbSNP:371108812
766 766 a, g dbSNP:760053342
767 767 g, t dbSNP:767954284
768 768 a, g dbSNP:375035296
779 779 c, t dbSNP:756270854
781 781 a, g dbSNP:764356536
786 786 a, c, t dbSNP:754101715
788 788 c, g dbSNP:778739489
789 789 a, c dbSNP:745700208
793 793 a, g dbSNP:189775061
794 794 c, t dbSNP:563121954
801 801 c, t dbSNP:746449281
802 802 a, g dbSNP:768167384
809 809 c, t dbSNP:200436414
811 811 a, g dbSNP:747791938
814 814 g, t dbSNP:769368895
826 826 c, t dbSNP:202242218
832 832 a, g dbSNP:551617369
856 856 g, t dbSNP:141911577
869 869 c, t dbSNP:192883541
906 906 a, g dbSNP:150610526
942 942 a, g dbSNP:184441242
983 983 c, g dbSNP:535398997
1018 1018 c, g dbSNP:769281334
1019 1019 a, c dbSNP:550360369
1030 1030 c, g dbSNP:78182250
1098 1098 c, t dbSNP:77450727
1113 1113 c, t dbSNP:771751685
1114 1114 g, t dbSNP:188830852
1145 1145 g, t dbSNP:564449
1182 1182 c, t dbSNP:533509296
1183 1183 a, g dbSNP:564197288
1193 1193 a, c dbSNP:574378882
1225 1225 a, g dbSNP:114461287
1257 1257 c, g dbSNP:563055654
1318 1318 c, g dbSNP:578191720
1323 1323 a, c dbSNP:545605665
1326 1326 a, t dbSNP:560335826

Target ORF information:

RefSeq Version NM_000799
Organism Homo sapiens (human)
Definition Homo sapiens erythropoietin (EPO), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000799

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCCCTGCT GTCGCTCCCT 
CTGGGCCTCC CAGTCCTGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATATCACGA CGGGCTGTGC TGAACACTGC
AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
ATGGAGGTCG GGCAGCAGGC CGTAGAAGTC TGGCAGGGCC TGGCCCTGCT GTCGGAAGCT
GTCCTGCGGG GCCAGGCCCT GTTGGTCAAC TCTTCCCAGC CGTGGGAGCC CCTGCAGCTG
CATGTGGATA AAGCCGTCAG TGGCCTTCGC AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
GCCCAGAAGG AAGCCATCTC CCCTCCAGAT GCGGCCTCAG CTGCTCCACT CCGAACAATC
ACTGCTGACA CTTTCCGCAA ACTCTTCCGA GTCTACTCCA ATTTCCTCCG GGGAAAGCTG
AAGCTGTACA CAGGGGAGGC CTGCAGGACA GGGGACAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Paraneoplastic hormones: parathyroid hormone-related protein (PTHrP) and erythropoietin (EPO) are related to vascular endothelial growth factor (VEGF) expression in clear cell renal cell carcinoma
Tumour Biol. 34 (6), 3471-3476 (2013)
Feng CC, Ding GX, Song NH, Li X, Wu Z, Jiang HW and Ding Q.


book

Erythropoietin is involved in the angiogenic potential of bone marrow macrophages in multiple myeloma
Angiogenesis 16 (4), 963-973 (2013)
De Luisi A, Binetti L, Ria R, Ruggieri S, Berardi S, Catacchio I, Racanelli V, Pavone V, Rossini B, Vacca A and Ribatti D.


book

Asymmetric dimethylarginine in response to recombinant tissue-type plasminogen activator and erythropoietin in acute stroke
Stroke 44 (8), 2128-2133 (2013)
Worthmann H, Martens-Lobenhoffer J, Joumaah M, Li N, Lichtinghagen R, Hecker H, Kielstein JT, Ehrenreich H, Bode-Boger SM and Weissenborn K.


book

The role and regulation of secretion of erythropoietin in pregnancy
Med Wieku Rozwoj 17 (3), 270-275 (2013)
Kowalska-Kanka A, Maciejewski T and Niemiec KT.


book

Urine erythropoietin level is associated with kidney and brain injury in critically ill neonates
Neonatology 104 (2), 87-94 (2013)
Li Y, Xiao Z, Yan J, Li X, Wang Q, Zhu H, Pan J, Zhu X, Wang J and Feng X.


book

Structures and functional roles of the sugar chains of human erythropoietins
Glycobiology 1 (4), 337-346 (1991)
Takeuchi M and Kobata A.


book

Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein
Science 242 (4884), 1412-1415 (1988)
Goldberg MA, Dunning SP and Bunn HF.


book

Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry
Biochemistry 27 (23), 8618-8626 (1988)
Sasaki H, Ochi N, Dell A and Fukuda M.


book

Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese hamster ovary cells
J. Biol. Chem. 263 (8), 3657-3663 (1988)
Takeuchi M, Takasaki S, Miyazaki H, Kato T, Hoshi S, Kochibe N and Kobata A.


book

Regional assignment of the erythropoietin gene to human chromosome region 7pter----q22
Cytogenet. Cell Genet. 42 (4), 214-218 (1986)
Watkins,P.C., Eddy,R., Hoffman,N., Stanislovitis,P., Beck,A.K., Galli,J., Vellucci,V., Gusella,J.F. and Shows,T.B.


 
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