Email to GenScript

AKT1 v-akt murine thymoma viral oncogene homolog 1 [Homo sapiens (human)]

Gene Symbol AKT1
Entrez Gene ID 207
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q32.32

Summary The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]. lac of sum
Disorder MIM:

164730

Disorder Html: Breast cancer, somatic, 114480 (3); Colorectal cancer, somatic,





The following AKT1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKT1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25680 XM_011536543 PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319
OHu25680 XM_005267401 PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319
OHu56223 XM_011536544 PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu25680 NM_005163 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319
OHu25680 NM_001014431 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319
OHu25680 NM_001014432 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu25680
Accession Version XM_011536543.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011534845.1
CDS668..2110
Misc Feature(1)677..1000(+)
Misc Feature(2)707..925(+)
Misc Feature(3)1037..2104(+)
Misc Feature(4)1115..1891(+)
Misc Feature(5)1133..1993(+)
Misc Feature(6)1133..1981(+)
Misc Feature(7)1145..1993(+)
Misc Feature(8)1538..1609(+)
Misc Feature(9)2015..2017(+)
Misc Feature(10)2072..2089(+)
Translation
Position Chain Variation Link
9 9 a, g dbSNP:549290087
38 38 c, t dbSNP:529626374
72 72 c, g dbSNP:183958050
74 74 -, ca dbSNP:747397159
126 126 c, t dbSNP:193056970
127 127 c, t dbSNP:532287543
135 135 a, t dbSNP:111540130
141 141 -, ga dbSNP:747556233
180 180 -, ct dbSNP:536585337
191 191 a, g dbSNP:533170341
193 193 a, g dbSNP:756125374
194 194 c, g dbSNP:545163265
212 212 c, t dbSNP:148414930
245 245 -, t dbSNP:533224900
262 262 c, t dbSNP:570528119
276 276 c, g dbSNP:61757056
281 281 a, c dbSNP:527577747
295 295 a, c dbSNP:573895159
298 298 c, g dbSNP:556808938
315 315 -, gcggc dbSNP:561765265
323 323 c, g dbSNP:537048919
325 325 a, g, t dbSNP:112844617
335 335 c, t dbSNP:187395973
365 365 -, g dbSNP:541422835
374 374 c, g dbSNP:61757057
388 388 a, g dbSNP:535147435
414 414 c, g dbSNP:548761391
424 424 a, g dbSNP:190916256
446 446 a, g dbSNP:778182689
475 475 a, g dbSNP:187239247
501 501 c, t dbSNP:756176055
502 502 c, t dbSNP:147187121
510 510 a, t dbSNP:748239540
511 511 a, c dbSNP:577532120
520 520 c, t dbSNP:564014530
576 576 a, g dbSNP:541421272
603 603 a, c dbSNP:371297714
606 606 a, g dbSNP:11555428
614 614 c, t dbSNP:753859473
615 615 a, g dbSNP:548942912
620 620 a, g dbSNP:370260488
621 621 c, t dbSNP:757627335
622 622 a, g dbSNP:34790457
623 623 c, g dbSNP:34757714
627 627 c, t dbSNP:763526322
628 628 -, agcc dbSNP:757126193
634 634 a, g dbSNP:752773454
635 635 c, g dbSNP:765349412
637 637 c, g dbSNP:373561553
638 638 c, t dbSNP:369911858
639 639 a, g dbSNP:181751951
640 640 c, t dbSNP:761545130
641 641 a, c, g dbSNP:768619756
647 647 a, g dbSNP:749225421
649 649 c, t dbSNP:779484182
651 651 c, t dbSNP:769337829
652 652 a, c, g dbSNP:780836176
657 657 c, t dbSNP:757729900
660 660 c, g, t dbSNP:28730786
661 661 a, g dbSNP:113993465
673 673 c, t dbSNP:371534192
674 674 a, g dbSNP:140532443
676 676 c, t dbSNP:56217512
677 677 a, g dbSNP:754031503
680 680 a, g dbSNP:766546254
700 700 a, g dbSNP:760853972
711 711 a, g dbSNP:368797346
716 716 a, g dbSNP:121434592
716 716 -, g dbSNP:34409589
718 718 a, g dbSNP:779114873
740 740 c, t dbSNP:397514644
745 745 c, t dbSNP:769253794
748 748 c, t dbSNP:749781543
759 759 a, g dbSNP:780173607
763 763 c, g, t dbSNP:201636005
768 768 a, c dbSNP:750653493
773 773 a, g dbSNP:781339141
774 774 c, t dbSNP:758157217
781 781 c, t dbSNP:752588225
782 782 a, g dbSNP:764931115
787 787 a, g dbSNP:370287382
788 788 c, t dbSNP:753765116
789 789 a, g dbSNP:766000895
793 793 a, g dbSNP:375645311
794 794 c, t dbSNP:11555436
805 805 a, c dbSNP:146875699
808 808 a, g dbSNP:532268608
809 809 c, t dbSNP:374093099
810 810 a, g dbSNP:774836044
816 816 c, t dbSNP:768898540
820 820 c, t dbSNP:749796064
823 823 c, t dbSNP:780477141
838 838 a, g dbSNP:769881484
841 841 a, g dbSNP:560243859
847 847 c, t dbSNP:767058127
853 853 a, g dbSNP:756659788
861 861 c, t dbSNP:751243134
862 862 a, g dbSNP:768384015
866 866 c, t dbSNP:762559261
872 872 c, t dbSNP:775892860
873 873 a, g dbSNP:183989506
881 881 a, g dbSNP:760044481
892 892 c, g dbSNP:587778019
894 894 a, g dbSNP:771065764
904 904 a, g dbSNP:747020429
913 913 c, t dbSNP:773311402
923 923 c, t dbSNP:772473246
924 924 a, g dbSNP:773971502
928 928 c, t dbSNP:748365698
934 934 c, t dbSNP:142646145
953 953 c, t dbSNP:760536822
961 961 a, g dbSNP:766755485
968 968 a, g dbSNP:761151169
970 970 c, t dbSNP:750890213
982 982 c, t dbSNP:767715614
985 985 c, g dbSNP:761930839
990 990 a, c dbSNP:774745066
992 992 a, g dbSNP:768800433
1012 1012 a, g dbSNP:147664316
1016 1016 -, gag dbSNP:768025881
1016 1016 a, g dbSNP:776345122
1028 1028 c, t dbSNP:369198922
1032 1032 c, t dbSNP:746934495
1033 1033 a, g dbSNP:777639707
1041 1041 c, t dbSNP:759031755
1054 1054 a, g dbSNP:747700076
1073 1073 a, g dbSNP:778376616
1094 1094 c, t dbSNP:142843688
1097 1097 c, t dbSNP:375990114
1099 1099 c, t dbSNP:17846825
1105 1105 c, g, t dbSNP:145780379
1107 1107 c, t dbSNP:758456890
1111 1111 c, t dbSNP:752894366
1120 1120 a, g dbSNP:765537155
1124 1124 c, t dbSNP:149803079
1136 1136 c, g dbSNP:568870136
1138 1138 a, c, t dbSNP:767298307
1150 1150 c, t dbSNP:139904675
1167 1167 c, t dbSNP:11555433
1177 1177 g, t dbSNP:774284624
1179 1179 c, t dbSNP:768070795
1182 1182 c, t dbSNP:748789094
1192 1192 c, t dbSNP:774828129
1200 1200 c, t dbSNP:769619023
1221 1221 g, t dbSNP:745500951
1225 1225 c, t dbSNP:34670300
1228 1228 g, t dbSNP:757639898
1229 1229 a, g dbSNP:144128670
1237 1237 c, t dbSNP:780207480
1245 1245 c, t dbSNP:756293014
1246 1246 c, g dbSNP:139453090
1254 1254 a, t dbSNP:751416672
1258 1258 c, t dbSNP:763782135
1267 1267 c, t dbSNP:61761191
1268 1268 a, g dbSNP:774360915
1271 1271 c, t dbSNP:2230506
1273 1273 a, g dbSNP:759079296
1282 1282 c, t dbSNP:368770666
1297 1297 c, g dbSNP:776250457
1309 1309 a, g dbSNP:765986469
1311 1311 a, g dbSNP:760384228
1312 1312 c, t dbSNP:772685079
1314 1314 -, ct dbSNP:770828544
1327 1327 c, t dbSNP:141168205
1328 1328 c, g dbSNP:762040581
1331 1331 c, t dbSNP:551254461
1332 1332 a, g dbSNP:369698909
1336 1336 c, g dbSNP:376531915
1347 1347 c, t dbSNP:775612804
1354 1354 c, t dbSNP:769696222
1360 1360 c, t dbSNP:767346459
1361 1361 a, g dbSNP:781232725
1363 1363 a, g dbSNP:201006619
1366 1366 c, t dbSNP:371786809
1367 1367 a, g dbSNP:778838358
1370 1370 c, g dbSNP:770980034
1372 1372 g, t dbSNP:769090254
1386 1386 c, g dbSNP:773520823
1388 1388 c, t dbSNP:768606668
1393 1393 a, g dbSNP:1130233
1403 1403 g, t dbSNP:779874420
1405 1405 c, t dbSNP:368667384
1413 1413 a, g dbSNP:745809388
1418 1418 c, t dbSNP:780571834
1419 1419 a, g dbSNP:756697784
1425 1425 a, g dbSNP:751232562
1430 1430 a, g dbSNP:375395037
1448 1448 c, g dbSNP:758025607
1450 1450 a, g dbSNP:143933508
1453 1453 c, g dbSNP:371467719
1456 1456 c, t dbSNP:759966382
1457 1457 c, t dbSNP:754317207
1464 1464 c, t dbSNP:549083521
1465 1465 a, g dbSNP:367635775
1474 1474 c, t dbSNP:201044857
1477 1477 a, g dbSNP:2230508
1486 1486 c, g dbSNP:762108714
1495 1495 a, g dbSNP:775212616
1522 1522 c, t dbSNP:149484260
1529 1529 a, g dbSNP:767699129
1543 1543 c, t dbSNP:761942512
1546 1546 c, t dbSNP:139200289
1552 1552 a, g dbSNP:373838774
1573 1573 c, t dbSNP:746001369
1597 1597 c, t dbSNP:146847196
1606 1606 c, t dbSNP:776378715
1615 1615 a, g dbSNP:751320716
1616 1616 -, g dbSNP:780293460
1618 1618 a, c dbSNP:17846831
1621 1621 c, t dbSNP:746944273
1623 1623 a, g dbSNP:12881616
1638 1638 a, g dbSNP:746397537
1640 1640 a, g dbSNP:781749630
1645 1645 c, t dbSNP:55874341
1654 1654 a, g dbSNP:751709437
1657 1657 a, g dbSNP:777711698
1672 1672 c, g dbSNP:758651475
1687 1687 c, t dbSNP:144088506
1699 1699 c, t dbSNP:56289559
1717 1717 c, t dbSNP:760425021
1720 1720 c, t dbSNP:376942361
1726 1726 c, t dbSNP:767544462
1729 1729 c, t dbSNP:761646283
1737 1737 c, t dbSNP:11555432
1754 1754 a, g dbSNP:372502847
1759 1759 a, g dbSNP:773975370
1766 1766 a, c, t dbSNP:762705090
1767 1767 a, g dbSNP:369520527
1774 1774 a, g dbSNP:150399268
1775 1775 c, t dbSNP:549370342
1776 1776 a, g dbSNP:746272761
1779 1779 c, t dbSNP:781388586
1780 1780 a, c, g dbSNP:747408700
1789 1789 c, t dbSNP:374865919
1790 1790 a, g dbSNP:373253729
1801 1801 c, t dbSNP:369527283
1813 1813 a, c, g dbSNP:755174756
1816 1816 a, c, g dbSNP:767207905
1823 1823 -, aag dbSNP:758624702
1825 1825 a, g dbSNP:761810870
1829 1829 a, c dbSNP:11555431
1846 1846 c, t dbSNP:11555434
1856 1856 a, g dbSNP:531850885
1857 1857 a, c dbSNP:766798551
1861 1861 c, t dbSNP:151254374
1862 1862 a, g dbSNP:188580689
1865 1865 a, g dbSNP:773607483
1867 1867 a, g dbSNP:772629378
1873 1873 c, t dbSNP:748654766
1876 1876 a, g dbSNP:774414969
1881 1881 a, c dbSNP:768660759
1883 1883 c, t dbSNP:749544983
1884 1884 a, g dbSNP:143266084
1894 1894 c, t dbSNP:141303557
1895 1895 a, g dbSNP:146483593
1900 1900 c, t dbSNP:375901448
1910 1910 c, t dbSNP:777696700
1912 1912 c, t dbSNP:758476416
1918 1918 c, t dbSNP:139297659
1919 1919 a, g dbSNP:764863282
1923 1923 a, g dbSNP:754500025
1925 1925 a, g dbSNP:753360468
1936 1936 a, g dbSNP:201227909
1956 1956 c, t dbSNP:749186394
1957 1957 a, g dbSNP:779447501
1959 1959 c, t dbSNP:755597789
1960 1960 a, g dbSNP:750104452
1970 1970 a, c dbSNP:397514645
1972 1972 c, t dbSNP:780860941
1996 1996 a, g dbSNP:376893212
2005 2005 g, t dbSNP:751976958
2006 2006 a, g dbSNP:764514218
2011 2011 c, t dbSNP:758833789
2022 2022 a, c dbSNP:753190812
2026 2026 c, g dbSNP:765264778
2028 2028 a, g dbSNP:759702315
2031 2031 a, g dbSNP:754040453
2038 2038 c, g dbSNP:759902535
2040 2040 a, c, t dbSNP:587778018
2047 2047 c, t dbSNP:139394285
2048 2048 g, t dbSNP:11555435
2050 2050 a, g dbSNP:763831402
2053 2053 c, t dbSNP:762849643
2056 2056 a, c, t dbSNP:144112075
2057 2057 a, g dbSNP:745803788
2060 2060 c, g, t dbSNP:770370100
2061 2061 a, g dbSNP:113547523
2083 2083 c, t dbSNP:184263655
2088 2088 a, c dbSNP:757963528
2092 2092 a, g dbSNP:748603087
2098 2098 c, t dbSNP:200213561
2103 2103 c, t dbSNP:201291259
2104 2104 a, g dbSNP:555187157
2113 2113 c, t dbSNP:780299611
2114 2114 a, g dbSNP:756226133
2116 2116 c, t dbSNP:200508405
2117 2117 a, g dbSNP:192237287
2118 2118 a, g, t dbSNP:111793056
2120 2120 a, g dbSNP:752606488
2126 2126 c, t dbSNP:377339529
2127 2127 a, g dbSNP:373329736
2133 2133 c, t dbSNP:375585585
2134 2134 a, c, g dbSNP:370987171
2145 2145 a, g dbSNP:558891046
2147 2147 a, g dbSNP:539258432
2148 2148 -, a dbSNP:761112590
2157 2157 c, t dbSNP:368920620
2158 2158 a, g dbSNP:573353277
2159 2159 a, g dbSNP:11555430
2196 2196 c, t dbSNP:553657544
2220 2220 c, t dbSNP:17846821
2231 2231 c, t dbSNP:17846826
2246 2246 a, g dbSNP:548181379
2249 2249 c, t dbSNP:537945247
2280 2280 a, g dbSNP:373002859
2340 2340 a, c, t dbSNP:112281303
2359 2359 c, g dbSNP:61761249
2378 2378 c, t dbSNP:34558581
2385 2385 a, g dbSNP:532385857
2411 2411 c, t dbSNP:41307094
2423 2423 c, t dbSNP:770697761
2433 2433 a, c, g dbSNP:1130245
2439 2439 a, g dbSNP:137981458
2448 2448 a, c dbSNP:560947797
2454 2454 c, t dbSNP:544158185
2464 2464 -, tgtc dbSNP:575655519
2476 2476 a, g dbSNP:575222918
2478 2478 a, c dbSNP:34865723
2489 2489 -, tgtc dbSNP:756938164
2489 2489 a, t dbSNP:113613978
2501 2501 g, t dbSNP:35416681
2510 2510 a, t dbSNP:573418247
2511 2511 a, c dbSNP:553256558
2546 2546 a, g dbSNP:765101207
2547 2547 c, t dbSNP:539274348
2554 2554 c, t dbSNP:754575126
2606 2606 a, g dbSNP:574337857
2613 2613 a, c dbSNP:554616284
2630 2630 c, t dbSNP:537882189
2632 2632 a, t dbSNP:568759365
2644 2644 c, t dbSNP:570600044
2655 2655 g, t dbSNP:1804268
2664 2664 c, t dbSNP:1130295
2702 2702 a, g dbSNP:751165631
2724 2724 a, g dbSNP:780314185
2779 2779 a, g dbSNP:758739510
2785 2785 -, g dbSNP:566232803
2814 2814 a, g dbSNP:1130443
2845 2845 a, g dbSNP:552278695
2874 2874 c, t dbSNP:112114610
2875 2875 a, g dbSNP:566677791
2880 2880 -, acttgaccttttcg dbSNP:55707948
2888 2888 c, t dbSNP:559789852
2896 2896 -, ttgaccttttcgac, ttgacctttttgac dbSNP:3840005
2896 2896 c, g, t dbSNP:762049014
2897 2897 a, g dbSNP:186886987
2905 2905 c, t dbSNP:769089409
2909 2909 c, t dbSNP:749839506
2915 2915 c, t dbSNP:753950090
2918 2918 c, t dbSNP:764172888
2928 2928 c, t dbSNP:3803305
2930 2930 c, t dbSNP:567261290
2963 2963 c, t dbSNP:17846828
2964 2964 c, g dbSNP:58565216
2970 2970 a, g dbSNP:17846829
2984 2984 c, t dbSNP:183360200
2990 2990 c, t dbSNP:17102385
2996 2996 c, t dbSNP:528550066
2997 2997 a, g dbSNP:140320743
2999 2999 c, t dbSNP:773176237
3001 3001 c, t dbSNP:190605178
3006 3006 c, t dbSNP:11555429
3050 3050 a, g dbSNP:769719936
3079 3079 c, t dbSNP:1130526

Target ORF information:

RefSeq Version XM_011536543
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536543

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Accession Version XM_005267401.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005267458.1
CDS490..1932
Misc Feature(1)499..822(+)
Misc Feature(2)529..747(+)
Misc Feature(3)859..1926(+)
Misc Feature(4)937..1713(+)
Misc Feature(5)955..1815(+)
Misc Feature(6)955..1803(+)
Misc Feature(7)967..1815(+)
Misc Feature(8)1360..1431(+)
Misc Feature(9)1837..1839(+)
Misc Feature(10)1894..1911(+)
Translation
Position Chain Variation Link
9 9 a, g dbSNP:549290087
38 38 c, t dbSNP:529626374
72 72 c, g dbSNP:183958050
74 74 -, ca dbSNP:747397159
126 126 c, t dbSNP:193056970
127 127 c, t dbSNP:532287543
135 135 a, t dbSNP:111540130
141 141 -, ga dbSNP:747556233
180 180 -, ct dbSNP:536585337
191 191 a, g dbSNP:533170341
193 193 a, g dbSNP:756125374
194 194 c, g dbSNP:545163265
212 212 c, t dbSNP:148414930
245 245 -, t dbSNP:533224900
262 262 c, t dbSNP:570528119
276 276 c, g dbSNP:61757056
281 281 a, c dbSNP:527577747
295 295 a, c dbSNP:573895159
298 298 c, g dbSNP:556808938
315 315 -, gcggc dbSNP:561765265
323 323 c, g dbSNP:537048919
325 325 a, g, t dbSNP:112844617
335 335 c, t dbSNP:187395973
365 365 -, g dbSNP:541422835
374 374 c, g dbSNP:61757057
388 388 a, g dbSNP:535147435
425 425 a, c dbSNP:371297714
428 428 a, g dbSNP:11555428
436 436 c, t dbSNP:753859473
437 437 a, g dbSNP:548942912
442 442 a, g dbSNP:370260488
443 443 c, t dbSNP:757627335
444 444 a, g dbSNP:34790457
445 445 c, g dbSNP:34757714
449 449 c, t dbSNP:763526322
450 450 -, agcc dbSNP:757126193
456 456 a, g dbSNP:752773454
457 457 c, g dbSNP:765349412
459 459 c, g dbSNP:373561553
460 460 c, t dbSNP:369911858
461 461 a, g dbSNP:181751951
462 462 c, t dbSNP:761545130
463 463 a, c, g dbSNP:768619756
469 469 a, g dbSNP:749225421
471 471 c, t dbSNP:779484182
473 473 c, t dbSNP:769337829
474 474 a, c, g dbSNP:780836176
479 479 c, t dbSNP:757729900
482 482 c, g, t dbSNP:28730786
483 483 a, g dbSNP:113993465
495 495 c, t dbSNP:371534192
496 496 a, g dbSNP:140532443
498 498 c, t dbSNP:56217512
499 499 a, g dbSNP:754031503
502 502 a, g dbSNP:766546254
522 522 a, g dbSNP:760853972
533 533 a, g dbSNP:368797346
538 538 a, g dbSNP:121434592
538 538 -, g dbSNP:34409589
540 540 a, g dbSNP:779114873
562 562 c, t dbSNP:397514644
567 567 c, t dbSNP:769253794
570 570 c, t dbSNP:749781543
581 581 a, g dbSNP:780173607
585 585 c, g, t dbSNP:201636005
590 590 a, c dbSNP:750653493
595 595 a, g dbSNP:781339141
596 596 c, t dbSNP:758157217
603 603 c, t dbSNP:752588225
604 604 a, g dbSNP:764931115
609 609 a, g dbSNP:370287382
610 610 c, t dbSNP:753765116
611 611 a, g dbSNP:766000895
615 615 a, g dbSNP:375645311
616 616 c, t dbSNP:11555436
627 627 a, c dbSNP:146875699
630 630 a, g dbSNP:532268608
631 631 c, t dbSNP:374093099
632 632 a, g dbSNP:774836044
638 638 c, t dbSNP:768898540
642 642 c, t dbSNP:749796064
645 645 c, t dbSNP:780477141
660 660 a, g dbSNP:769881484
663 663 a, g dbSNP:560243859
669 669 c, t dbSNP:767058127
675 675 a, g dbSNP:756659788
683 683 c, t dbSNP:751243134
684 684 a, g dbSNP:768384015
688 688 c, t dbSNP:762559261
694 694 c, t dbSNP:775892860
695 695 a, g dbSNP:183989506
703 703 a, g dbSNP:760044481
714 714 c, g dbSNP:587778019
716 716 a, g dbSNP:771065764
726 726 a, g dbSNP:747020429
735 735 c, t dbSNP:773311402
745 745 c, t dbSNP:772473246
746 746 a, g dbSNP:773971502
750 750 c, t dbSNP:748365698
756 756 c, t dbSNP:142646145
775 775 c, t dbSNP:760536822
783 783 a, g dbSNP:766755485
790 790 a, g dbSNP:761151169
792 792 c, t dbSNP:750890213
804 804 c, t dbSNP:767715614
807 807 c, g dbSNP:761930839
812 812 a, c dbSNP:774745066
814 814 a, g dbSNP:768800433
834 834 a, g dbSNP:147664316
838 838 -, gag dbSNP:768025881
838 838 a, g dbSNP:776345122
850 850 c, t dbSNP:369198922
854 854 c, t dbSNP:746934495
855 855 a, g dbSNP:777639707
863 863 c, t dbSNP:759031755
876 876 a, g dbSNP:747700076
895 895 a, g dbSNP:778376616
916 916 c, t dbSNP:142843688
919 919 c, t dbSNP:375990114
921 921 c, t dbSNP:17846825
927 927 c, g, t dbSNP:145780379
929 929 c, t dbSNP:758456890
933 933 c, t dbSNP:752894366
942 942 a, g dbSNP:765537155
946 946 c, t dbSNP:149803079
958 958 c, g dbSNP:568870136
960 960 a, c, t dbSNP:767298307
972 972 c, t dbSNP:139904675
989 989 c, t dbSNP:11555433
999 999 g, t dbSNP:774284624
1001 1001 c, t dbSNP:768070795
1004 1004 c, t dbSNP:748789094
1014 1014 c, t dbSNP:774828129
1022 1022 c, t dbSNP:769619023
1043 1043 g, t dbSNP:745500951
1047 1047 c, t dbSNP:34670300
1050 1050 g, t dbSNP:757639898
1051 1051 a, g dbSNP:144128670
1059 1059 c, t dbSNP:780207480
1067 1067 c, t dbSNP:756293014
1068 1068 c, g dbSNP:139453090
1076 1076 a, t dbSNP:751416672
1080 1080 c, t dbSNP:763782135
1089 1089 c, t dbSNP:61761191
1090 1090 a, g dbSNP:774360915
1093 1093 c, t dbSNP:2230506
1095 1095 a, g dbSNP:759079296
1104 1104 c, t dbSNP:368770666
1119 1119 c, g dbSNP:776250457
1131 1131 a, g dbSNP:765986469
1133 1133 a, g dbSNP:760384228
1134 1134 c, t dbSNP:772685079
1136 1136 -, ct dbSNP:770828544
1149 1149 c, t dbSNP:141168205
1150 1150 c, g dbSNP:762040581
1153 1153 c, t dbSNP:551254461
1154 1154 a, g dbSNP:369698909
1158 1158 c, g dbSNP:376531915
1169 1169 c, t dbSNP:775612804
1176 1176 c, t dbSNP:769696222
1182 1182 c, t dbSNP:767346459
1183 1183 a, g dbSNP:781232725
1185 1185 a, g dbSNP:201006619
1188 1188 c, t dbSNP:371786809
1189 1189 a, g dbSNP:778838358
1192 1192 c, g dbSNP:770980034
1194 1194 g, t dbSNP:769090254
1208 1208 c, g dbSNP:773520823
1210 1210 c, t dbSNP:768606668
1215 1215 a, g dbSNP:1130233
1225 1225 g, t dbSNP:779874420
1227 1227 c, t dbSNP:368667384
1235 1235 a, g dbSNP:745809388
1240 1240 c, t dbSNP:780571834
1241 1241 a, g dbSNP:756697784
1247 1247 a, g dbSNP:751232562
1252 1252 a, g dbSNP:375395037
1270 1270 c, g dbSNP:758025607
1272 1272 a, g dbSNP:143933508
1275 1275 c, g dbSNP:371467719
1278 1278 c, t dbSNP:759966382
1279 1279 c, t dbSNP:754317207
1286 1286 c, t dbSNP:549083521
1287 1287 a, g dbSNP:367635775
1296 1296 c, t dbSNP:201044857
1299 1299 a, g dbSNP:2230508
1308 1308 c, g dbSNP:762108714
1317 1317 a, g dbSNP:775212616
1344 1344 c, t dbSNP:149484260
1351 1351 a, g dbSNP:767699129
1365 1365 c, t dbSNP:761942512
1368 1368 c, t dbSNP:139200289
1374 1374 a, g dbSNP:373838774
1395 1395 c, t dbSNP:746001369
1419 1419 c, t dbSNP:146847196
1428 1428 c, t dbSNP:776378715
1437 1437 a, g dbSNP:751320716
1438 1438 -, g dbSNP:780293460
1440 1440 a, c dbSNP:17846831
1443 1443 c, t dbSNP:746944273
1445 1445 a, g dbSNP:12881616
1460 1460 a, g dbSNP:746397537
1462 1462 a, g dbSNP:781749630
1467 1467 c, t dbSNP:55874341
1476 1476 a, g dbSNP:751709437
1479 1479 a, g dbSNP:777711698
1494 1494 c, g dbSNP:758651475
1509 1509 c, t dbSNP:144088506
1521 1521 c, t dbSNP:56289559
1539 1539 c, t dbSNP:760425021
1542 1542 c, t dbSNP:376942361
1548 1548 c, t dbSNP:767544462
1551 1551 c, t dbSNP:761646283
1559 1559 c, t dbSNP:11555432
1576 1576 a, g dbSNP:372502847
1581 1581 a, g dbSNP:773975370
1588 1588 a, c, t dbSNP:762705090
1589 1589 a, g dbSNP:369520527
1596 1596 a, g dbSNP:150399268
1597 1597 c, t dbSNP:549370342
1598 1598 a, g dbSNP:746272761
1601 1601 c, t dbSNP:781388586
1602 1602 a, c, g dbSNP:747408700
1611 1611 c, t dbSNP:374865919
1612 1612 a, g dbSNP:373253729
1623 1623 c, t dbSNP:369527283
1635 1635 a, c, g dbSNP:755174756
1638 1638 a, c, g dbSNP:767207905
1645 1645 -, aag dbSNP:758624702
1647 1647 a, g dbSNP:761810870
1651 1651 a, c dbSNP:11555431
1668 1668 c, t dbSNP:11555434
1678 1678 a, g dbSNP:531850885
1679 1679 a, c dbSNP:766798551
1683 1683 c, t dbSNP:151254374
1684 1684 a, g dbSNP:188580689
1687 1687 a, g dbSNP:773607483
1689 1689 a, g dbSNP:772629378
1695 1695 c, t dbSNP:748654766
1698 1698 a, g dbSNP:774414969
1703 1703 a, c dbSNP:768660759
1705 1705 c, t dbSNP:749544983
1706 1706 a, g dbSNP:143266084
1716 1716 c, t dbSNP:141303557
1717 1717 a, g dbSNP:146483593
1722 1722 c, t dbSNP:375901448
1732 1732 c, t dbSNP:777696700
1734 1734 c, t dbSNP:758476416
1740 1740 c, t dbSNP:139297659
1741 1741 a, g dbSNP:764863282
1745 1745 a, g dbSNP:754500025
1747 1747 a, g dbSNP:753360468
1758 1758 a, g dbSNP:201227909
1778 1778 c, t dbSNP:749186394
1779 1779 a, g dbSNP:779447501
1781 1781 c, t dbSNP:755597789
1782 1782 a, g dbSNP:750104452
1792 1792 a, c dbSNP:397514645
1794 1794 c, t dbSNP:780860941
1818 1818 a, g dbSNP:376893212
1827 1827 g, t dbSNP:751976958
1828 1828 a, g dbSNP:764514218
1833 1833 c, t dbSNP:758833789
1844 1844 a, c dbSNP:753190812
1848 1848 c, g dbSNP:765264778
1850 1850 a, g dbSNP:759702315
1853 1853 a, g dbSNP:754040453
1860 1860 c, g dbSNP:759902535
1862 1862 a, c, t dbSNP:587778018
1869 1869 c, t dbSNP:139394285
1870 1870 g, t dbSNP:11555435
1872 1872 a, g dbSNP:763831402
1875 1875 c, t dbSNP:762849643
1878 1878 a, c, t dbSNP:144112075
1879 1879 a, g dbSNP:745803788
1882 1882 c, g, t dbSNP:770370100
1883 1883 a, g dbSNP:113547523
1905 1905 c, t dbSNP:184263655
1910 1910 a, c dbSNP:757963528
1914 1914 a, g dbSNP:748603087
1920 1920 c, t dbSNP:200213561
1925 1925 c, t dbSNP:201291259
1926 1926 a, g dbSNP:555187157
1935 1935 c, t dbSNP:780299611
1936 1936 a, g dbSNP:756226133
1938 1938 c, t dbSNP:200508405
1939 1939 a, g dbSNP:192237287
1940 1940 a, g, t dbSNP:111793056
1942 1942 a, g dbSNP:752606488
1948 1948 c, t dbSNP:377339529
1949 1949 a, g dbSNP:373329736
1955 1955 c, t dbSNP:375585585
1956 1956 a, c, g dbSNP:370987171
1967 1967 a, g dbSNP:558891046
1969 1969 a, g dbSNP:539258432
1970 1970 -, a dbSNP:761112590
1979 1979 c, t dbSNP:368920620
1980 1980 a, g dbSNP:573353277
1981 1981 a, g dbSNP:11555430
2018 2018 c, t dbSNP:553657544
2042 2042 c, t dbSNP:17846821
2053 2053 c, t dbSNP:17846826
2068 2068 a, g dbSNP:548181379
2071 2071 c, t dbSNP:537945247
2102 2102 a, g dbSNP:373002859
2162 2162 a, c, t dbSNP:112281303
2181 2181 c, g dbSNP:61761249
2200 2200 c, t dbSNP:34558581
2207 2207 a, g dbSNP:532385857
2233 2233 c, t dbSNP:41307094
2245 2245 c, t dbSNP:770697761
2255 2255 a, c, g dbSNP:1130245
2261 2261 a, g dbSNP:137981458
2270 2270 a, c dbSNP:560947797
2276 2276 c, t dbSNP:544158185
2286 2286 -, tgtc dbSNP:575655519
2298 2298 a, g dbSNP:575222918
2300 2300 a, c dbSNP:34865723
2311 2311 -, tgtc dbSNP:756938164
2311 2311 a, t dbSNP:113613978
2323 2323 g, t dbSNP:35416681
2332 2332 a, t dbSNP:573418247
2333 2333 a, c dbSNP:553256558
2368 2368 a, g dbSNP:765101207
2369 2369 c, t dbSNP:539274348
2376 2376 c, t dbSNP:754575126
2428 2428 a, g dbSNP:574337857
2435 2435 a, c dbSNP:554616284
2452 2452 c, t dbSNP:537882189
2454 2454 a, t dbSNP:568759365
2466 2466 c, t dbSNP:570600044
2477 2477 g, t dbSNP:1804268
2486 2486 c, t dbSNP:1130295
2524 2524 a, g dbSNP:751165631
2546 2546 a, g dbSNP:780314185
2601 2601 a, g dbSNP:758739510
2607 2607 -, g dbSNP:566232803
2636 2636 a, g dbSNP:1130443
2667 2667 a, g dbSNP:552278695
2696 2696 c, t dbSNP:112114610
2697 2697 a, g dbSNP:566677791
2702 2702 -, acttgaccttttcg dbSNP:55707948
2710 2710 c, t dbSNP:559789852
2718 2718 -, ttgaccttttcgac, ttgacctttttgac dbSNP:3840005
2718 2718 c, g, t dbSNP:762049014
2719 2719 a, g dbSNP:186886987
2727 2727 c, t dbSNP:769089409
2731 2731 c, t dbSNP:749839506
2737 2737 c, t dbSNP:753950090
2740 2740 c, t dbSNP:764172888
2750 2750 c, t dbSNP:3803305
2752 2752 c, t dbSNP:567261290
2785 2785 c, t dbSNP:17846828
2786 2786 c, g dbSNP:58565216
2792 2792 a, g dbSNP:17846829
2806 2806 c, t dbSNP:183360200
2812 2812 c, t dbSNP:17102385
2818 2818 c, t dbSNP:528550066
2819 2819 a, g dbSNP:140320743
2821 2821 c, t dbSNP:773176237
2823 2823 c, t dbSNP:190605178
2828 2828 c, t dbSNP:11555429
2872 2872 a, g dbSNP:769719936
2901 2901 c, t dbSNP:1130526

Target ORF information:

RefSeq Version XM_005267401
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005267401

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56223
Accession Version XM_011536544.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
GGACCCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011534846.1
CDS1482..2750
Misc Feature(1)1491..1814(+)
Misc Feature(2)1521..1739(+)
Misc Feature(3)1851..2741(+)
Misc Feature(4)1929..2705(+)
Misc Feature(5)1947..2357(+)
Translation
Position Chain Variation Link
9 9 a, g dbSNP:549290087
38 38 c, t dbSNP:529626374
72 72 c, g dbSNP:183958050
74 74 -, ca dbSNP:747397159
126 126 c, t dbSNP:193056970
127 127 c, t dbSNP:532287543
135 135 a, t dbSNP:111540130
141 141 -, ga dbSNP:747556233
180 180 -, ct dbSNP:536585337
191 191 a, g dbSNP:533170341
193 193 a, g dbSNP:756125374
194 194 c, g dbSNP:545163265
212 212 c, t dbSNP:148414930
245 245 -, t dbSNP:533224900
262 262 c, t dbSNP:570528119
276 276 c, g dbSNP:61757056
281 281 a, c dbSNP:527577747
295 295 a, c dbSNP:573895159
298 298 c, g dbSNP:556808938
315 315 -, gcggc dbSNP:561765265
323 323 c, g dbSNP:537048919
325 325 a, g, t dbSNP:112844617
335 335 c, t dbSNP:187395973
365 365 -, g dbSNP:541422835
374 374 c, g dbSNP:61757057
388 388 a, g dbSNP:535147435
427 427 c, g dbSNP:566026331
429 429 c, t dbSNP:549572762
447 447 a, g dbSNP:529441227
490 490 a, g dbSNP:570333396
533 533 g, t dbSNP:562253211
565 565 a, g dbSNP:28634999
606 606 c, t dbSNP:761828731
618 618 c, t dbSNP:577389130
619 619 -, ag dbSNP:753401705
670 670 a, g dbSNP:533656219
673 673 g, t dbSNP:149275766
689 689 c, t dbSNP:182387027
711 711 a, g dbSNP:528221523
715 715 c, t dbSNP:559149413
720 720 a, g dbSNP:542828239
742 742 c, g dbSNP:573706022
770 770 c, t dbSNP:564969892
795 795 a, g dbSNP:190062821
823 823 a, g dbSNP:543360576
838 838 a, g dbSNP:757406059
862 862 g, t dbSNP:577494399
868 868 c, t dbSNP:185645727
883 883 g, t dbSNP:554049991
912 912 -, tgc dbSNP:564615537
920 920 a, g dbSNP:61995962
924 924 g, t dbSNP:534675280
936 936 a, g dbSNP:112005795
961 961 a, g dbSNP:776653960
968 968 a, c dbSNP:182741149
974 974 a, c, g dbSNP:138573028
1005 1005 a, g dbSNP:570304277
1036 1036 a, g dbSNP:550393268
1047 1047 g, t dbSNP:775026517
1061 1061 g, t dbSNP:540162615
1078 1078 g, t dbSNP:536323085
1086 1086 c, t dbSNP:771732248
1104 1104 c, g dbSNP:571141893
1132 1132 g, t dbSNP:1130214
1138 1138 c, t dbSNP:528047226
1160 1160 a, g dbSNP:10138227
1165 1165 c, t dbSNP:771481743
1228 1228 c, g dbSNP:548761391
1238 1238 a, g dbSNP:190916256
1260 1260 a, g dbSNP:778182689
1289 1289 a, g dbSNP:187239247
1315 1315 c, t dbSNP:756176055
1316 1316 c, t dbSNP:147187121
1324 1324 a, t dbSNP:748239540
1325 1325 a, c dbSNP:577532120
1334 1334 c, t dbSNP:564014530
1390 1390 a, g dbSNP:541421272
1417 1417 a, c dbSNP:371297714
1420 1420 a, g dbSNP:11555428
1428 1428 c, t dbSNP:753859473
1429 1429 a, g dbSNP:548942912
1434 1434 a, g dbSNP:370260488
1435 1435 c, t dbSNP:757627335
1436 1436 a, g dbSNP:34790457
1437 1437 c, g dbSNP:34757714
1441 1441 c, t dbSNP:763526322
1442 1442 -, agcc dbSNP:757126193
1448 1448 a, g dbSNP:752773454
1449 1449 c, g dbSNP:765349412
1451 1451 c, g dbSNP:373561553
1452 1452 c, t dbSNP:369911858
1453 1453 a, g dbSNP:181751951
1454 1454 c, t dbSNP:761545130
1455 1455 a, c, g dbSNP:768619756
1461 1461 a, g dbSNP:749225421
1463 1463 c, t dbSNP:779484182
1465 1465 c, t dbSNP:769337829
1466 1466 a, c, g dbSNP:780836176
1471 1471 c, t dbSNP:757729900
1474 1474 c, g, t dbSNP:28730786
1475 1475 a, g dbSNP:113993465
1487 1487 c, t dbSNP:371534192
1488 1488 a, g dbSNP:140532443
1490 1490 c, t dbSNP:56217512
1491 1491 a, g dbSNP:754031503
1494 1494 a, g dbSNP:766546254
1514 1514 a, g dbSNP:760853972
1525 1525 a, g dbSNP:368797346
1530 1530 a, g dbSNP:121434592
1530 1530 -, g dbSNP:34409589
1532 1532 a, g dbSNP:779114873
1554 1554 c, t dbSNP:397514644
1559 1559 c, t dbSNP:769253794
1562 1562 c, t dbSNP:749781543
1573 1573 a, g dbSNP:780173607
1577 1577 c, g, t dbSNP:201636005
1582 1582 a, c dbSNP:750653493
1587 1587 a, g dbSNP:781339141
1588 1588 c, t dbSNP:758157217
1595 1595 c, t dbSNP:752588225
1596 1596 a, g dbSNP:764931115
1601 1601 a, g dbSNP:370287382
1602 1602 c, t dbSNP:753765116
1603 1603 a, g dbSNP:766000895
1607 1607 a, g dbSNP:375645311
1608 1608 c, t dbSNP:11555436
1619 1619 a, c dbSNP:146875699
1622 1622 a, g dbSNP:532268608
1623 1623 c, t dbSNP:374093099
1624 1624 a, g dbSNP:774836044
1630 1630 c, t dbSNP:768898540
1634 1634 c, t dbSNP:749796064
1637 1637 c, t dbSNP:780477141
1652 1652 a, g dbSNP:769881484
1655 1655 a, g dbSNP:560243859
1661 1661 c, t dbSNP:767058127
1667 1667 a, g dbSNP:756659788
1675 1675 c, t dbSNP:751243134
1676 1676 a, g dbSNP:768384015
1680 1680 c, t dbSNP:762559261
1686 1686 c, t dbSNP:775892860
1687 1687 a, g dbSNP:183989506
1695 1695 a, g dbSNP:760044481
1706 1706 c, g dbSNP:587778019
1708 1708 a, g dbSNP:771065764
1718 1718 a, g dbSNP:747020429
1727 1727 c, t dbSNP:773311402
1737 1737 c, t dbSNP:772473246
1738 1738 a, g dbSNP:773971502
1742 1742 c, t dbSNP:748365698
1748 1748 c, t dbSNP:142646145
1767 1767 c, t dbSNP:760536822
1775 1775 a, g dbSNP:766755485
1782 1782 a, g dbSNP:761151169
1784 1784 c, t dbSNP:750890213
1796 1796 c, t dbSNP:767715614
1799 1799 c, g dbSNP:761930839
1804 1804 a, c dbSNP:774745066
1806 1806 a, g dbSNP:768800433
1826 1826 a, g dbSNP:147664316
1830 1830 -, gag dbSNP:768025881
1830 1830 a, g dbSNP:776345122
1842 1842 c, t dbSNP:369198922
1846 1846 c, t dbSNP:746934495
1847 1847 a, g dbSNP:777639707
1855 1855 c, t dbSNP:759031755
1868 1868 a, g dbSNP:747700076
1887 1887 a, g dbSNP:778376616
1908 1908 c, t dbSNP:142843688
1911 1911 c, t dbSNP:375990114
1913 1913 c, t dbSNP:17846825
1919 1919 c, g, t dbSNP:145780379
1921 1921 c, t dbSNP:758456890
1925 1925 c, t dbSNP:752894366
1934 1934 a, g dbSNP:765537155
1938 1938 c, t dbSNP:149803079
1950 1950 c, g dbSNP:568870136
1952 1952 a, c, t dbSNP:767298307
1964 1964 c, t dbSNP:139904675
1981 1981 c, t dbSNP:11555433
1991 1991 g, t dbSNP:774284624
1993 1993 c, t dbSNP:768070795
1996 1996 c, t dbSNP:748789094
2006 2006 c, t dbSNP:774828129
2014 2014 c, t dbSNP:769619023
2035 2035 g, t dbSNP:745500951
2039 2039 c, t dbSNP:34670300
2042 2042 g, t dbSNP:757639898
2043 2043 a, g dbSNP:144128670
2051 2051 c, t dbSNP:780207480
2059 2059 c, t dbSNP:756293014
2060 2060 c, g dbSNP:139453090
2068 2068 a, t dbSNP:751416672
2072 2072 c, t dbSNP:763782135
2081 2081 c, t dbSNP:61761191
2082 2082 a, g dbSNP:774360915
2085 2085 c, t dbSNP:2230506
2087 2087 a, g dbSNP:759079296
2096 2096 c, t dbSNP:368770666
2111 2111 c, g dbSNP:776250457
2123 2123 a, g dbSNP:765986469
2125 2125 a, g dbSNP:760384228
2126 2126 c, t dbSNP:772685079
2128 2128 -, ct dbSNP:770828544
2141 2141 c, t dbSNP:141168205
2142 2142 c, g dbSNP:762040581
2145 2145 c, t dbSNP:551254461
2146 2146 a, g dbSNP:369698909
2150 2150 c, g dbSNP:376531915
2161 2161 c, t dbSNP:775612804
2168 2168 c, t dbSNP:769696222
2174 2174 c, t dbSNP:767346459
2175 2175 a, g dbSNP:781232725
2177 2177 a, g dbSNP:201006619
2180 2180 c, t dbSNP:371786809
2181 2181 a, g dbSNP:778838358
2184 2184 c, g dbSNP:770980034
2186 2186 g, t dbSNP:769090254
2200 2200 c, g dbSNP:773520823
2202 2202 c, t dbSNP:768606668
2207 2207 a, g dbSNP:1130233
2217 2217 g, t dbSNP:779874420
2219 2219 c, t dbSNP:368667384
2227 2227 a, g dbSNP:745809388
2232 2232 c, t dbSNP:780571834
2233 2233 a, g dbSNP:756697784
2239 2239 a, g dbSNP:751232562
2244 2244 a, g dbSNP:375395037
2262 2262 c, g dbSNP:758025607
2264 2264 a, g dbSNP:143933508
2267 2267 c, g dbSNP:371467719
2270 2270 c, t dbSNP:759966382
2271 2271 c, t dbSNP:754317207
2278 2278 c, t dbSNP:549083521
2279 2279 a, g dbSNP:367635775
2288 2288 c, t dbSNP:201044857
2291 2291 a, g dbSNP:2230508
2300 2300 c, g dbSNP:762108714
2309 2309 a, g dbSNP:775212616
2336 2336 c, t dbSNP:149484260
2343 2343 a, g dbSNP:767699129
2357 2357 c, t dbSNP:761942512
2360 2360 c, t dbSNP:139200289
2366 2366 a, g dbSNP:373838774
2387 2387 c, t dbSNP:746001369
2411 2411 c, t dbSNP:146847196
2420 2420 c, t dbSNP:776378715
2429 2429 a, g dbSNP:751320716
2430 2430 -, g dbSNP:780293460
2432 2432 a, c dbSNP:17846831
2435 2435 c, t dbSNP:746944273
2437 2437 a, g dbSNP:12881616
2452 2452 a, g dbSNP:746397537
2454 2454 a, g dbSNP:781749630
2459 2459 c, t dbSNP:55874341
2468 2468 a, g dbSNP:751709437
2471 2471 a, g dbSNP:777711698
2486 2486 c, g dbSNP:758651475
2501 2501 c, t dbSNP:144088506
2513 2513 c, t dbSNP:56289559
2531 2531 c, t dbSNP:760425021
2534 2534 c, t dbSNP:376942361
2540 2540 c, t dbSNP:767544462
2543 2543 c, t dbSNP:761646283
2551 2551 c, t dbSNP:11555432
2568 2568 a, g dbSNP:372502847
2573 2573 a, g dbSNP:773975370
2580 2580 a, c, t dbSNP:762705090
2581 2581 a, g dbSNP:369520527
2588 2588 a, g dbSNP:150399268
2589 2589 c, t dbSNP:549370342
2590 2590 a, g dbSNP:746272761
2593 2593 c, t dbSNP:781388586
2594 2594 a, c, g dbSNP:747408700
2603 2603 c, t dbSNP:374865919
2604 2604 a, g dbSNP:373253729
2615 2615 c, t dbSNP:369527283
2627 2627 a, c, g dbSNP:755174756
2630 2630 a, c, g dbSNP:767207905
2637 2637 -, aag dbSNP:758624702
2639 2639 a, g dbSNP:761810870
2643 2643 a, c dbSNP:11555431
2660 2660 c, t dbSNP:11555434
2670 2670 a, g dbSNP:531850885
2671 2671 a, c dbSNP:766798551
2675 2675 c, t dbSNP:151254374
2676 2676 a, g dbSNP:188580689
2679 2679 a, g dbSNP:773607483
2681 2681 a, g dbSNP:772629378
2687 2687 c, t dbSNP:748654766
2690 2690 a, g dbSNP:774414969
2695 2695 a, c dbSNP:768660759
2697 2697 c, t dbSNP:749544983
2698 2698 a, g dbSNP:143266084
2708 2708 c, t dbSNP:141303557
2709 2709 a, g dbSNP:146483593
2714 2714 c, t dbSNP:375901448
2724 2724 c, t dbSNP:777696700
2726 2726 c, t dbSNP:758476416
2732 2732 c, t dbSNP:139297659
2733 2733 a, g dbSNP:764863282
2737 2737 a, g dbSNP:754500025
2739 2739 a, g dbSNP:753360468
2742 2742 a, g dbSNP:564089357
2749 2749 a, g dbSNP:768118092
2754 2754 c, t dbSNP:185374680
2769 2769 c, t dbSNP:61761246
2788 2788 c, g dbSNP:763869399
2796 2796 c, t dbSNP:561327131
2851 2851 c, t dbSNP:542005139
2869 2869 c, t dbSNP:77302659
2881 2881 c, t dbSNP:576294991
2905 2905 c, t dbSNP:755773106
2908 2908 a, g dbSNP:547916185
2912 2912 a, g dbSNP:546096971
2924 2924 a, g dbSNP:531298345
2938 2938 c, t dbSNP:554102976
2964 2964 c, t dbSNP:61761247
2969 2969 c, t dbSNP:774970645
2976 2976 c, g dbSNP:10141765
2978 2978 c, t dbSNP:372624026
2982 2982 a, c dbSNP:778432016
3011 3011 c, g dbSNP:766763375
3085 3085 a, g dbSNP:538093247
3127 3127 g, t dbSNP:763159591
3128 3128 -, g dbSNP:34326349
3170 3170 c, t dbSNP:115806317
3210 3210 c, t dbSNP:552482732
3215 3215 c, g dbSNP:2494731
3218 3218 c, t dbSNP:570373841
3303 3303 c, t dbSNP:769928859
3312 3312 c, t dbSNP:187915617
3324 3324 c, t dbSNP:527401955
3328 3328 a, c dbSNP:530462079
3356 3356 c, t dbSNP:748412919
3368 3368 c, t dbSNP:541942265
3377 3377 a, g dbSNP:776942425
3429 3429 c, t dbSNP:772039336
3450 3450 c, t dbSNP:745616456
3470 3470 a, c dbSNP:778972564
3477 3477 a, g dbSNP:546083181
3481 3481 a, g dbSNP:531562828
3490 3490 c, g dbSNP:562656821
3491 3491 a, g dbSNP:545796299
3494 3494 c, t dbSNP:7140735
3500 3500 c, g dbSNP:749196395
3515 3515 c, t dbSNP:118098425
3519 3519 c, g dbSNP:576436971
3527 3527 c, t dbSNP:540582046
3561 3561 g, t dbSNP:574734523
3563 3563 c, g dbSNP:752371856
3621 3621 c, t dbSNP:554693082
3641 3641 g, t dbSNP:540900119
3644 3644 c, t dbSNP:537928294
3674 3674 c, t dbSNP:762121960
3675 3675 a, g dbSNP:751887179
3676 3676 a, g dbSNP:764632008
3680 3680 g, t dbSNP:763502372
3681 3681 c, t dbSNP:775499085

Target ORF information:

RefSeq Version XM_011536544
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536544

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
GGACCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Accession Version NM_005163.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122894.1 and BC000479.2. This sequence is a reference standard in the RefSeqGene project. On Apr 6, 2005 this sequence version replaced gi:4885060. Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]. Transcript Variant: This variant (1) is the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122894.1, M63167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_005154.2
CDS555..1997
Misc Feature(1)516..518(+)
Misc Feature(2)564..887(+)
Misc Feature(3)594..812(+)
Misc Feature(4)594..611(+)
Misc Feature(5)594..596(+)
Misc Feature(6)612..614(+)
Misc Feature(7)621..629(+)
Misc Feature(8)924..1991(+)
Misc Feature(9)924..926(+)
Misc Feature(10)924..926(+)
Misc Feature(11)930..932(+)
Misc Feature(12)930..932(+)
Misc Feature(13)939..941(+)
Misc Feature(14)939..941(+)
Misc Feature(15)1002..1778(+)
Misc Feature(16)1020..1880(+)
Misc Feature(17)1020..1868(+)
Misc Feature(18)1032..1880(+)
Misc Feature(19)1080..1082(+)
Misc Feature(20)1236..1244(+)
Misc Feature(21)1425..1496(+)
Misc Feature(22)1476..1478(+)
Misc Feature(23)1476..1478(+)
Misc Feature(24)1476..1478(+)
Misc Feature(25)1476..1478(+)
Misc Feature(26)1476..1478(+)
Misc Feature(27)1476..1478(+)
Misc Feature(28)1497..1499(+)
Misc Feature(29)1530..1532(+)
Misc Feature(30)1902..1904(+)
Misc Feature(31)1902..1904(+)
Misc Feature(32)1938..1940(+)
Misc Feature(33)1959..1976(+)
Misc Feature(34)1971..1973(+)
Misc Feature(35)1971..1973(+)
Misc Feature(36)1971..1973(+)
Misc Feature(37)1971..1973(+)
Misc Feature(38)1971..1973(+)
Misc Feature(39)1971..1973(+)
Misc Feature(40)1971..1973(+)
Misc Feature(41)1974..1976(+)
Misc Feature(42)1974..1976(+)
Exon (1)1..475
Gene:AKT1
Gene Synonym:
Exon (2)476..600
Gene:AKT1
Gene Synonym:
Exon (3)601..729
Gene:AKT1
Gene Synonym:
Exon (4)730..841
Gene:AKT1
Gene Synonym:
Exon (5)842..989
Gene:AKT1
Gene Synonym:
Exon (6)990..1121
Gene:AKT1
Gene Synonym:
Exon (7)1122..1187
Gene:AKT1
Gene Synonym:
Exon (8)1188..1256
Gene:AKT1
Gene Synonym:
Exon (9)1257..1382
Gene:AKT1
Gene Synonym:
Exon (10)1383..1511
Gene:AKT1
Gene Synonym:
Exon (11)1512..1726
Gene:AKT1
Gene Synonym:
Exon (12)1727..1814
Gene:AKT1
Gene Synonym:
Exon (13)1815..1917
Gene:AKT1
Gene Synonym:
Exon (14)1918..2989
Gene:AKT1
Gene Synonym:
Translation
Position Chain Variation Link
9 9 a, g dbSNP:112005795
34 34 a, g dbSNP:776653960
41 41 a, c dbSNP:182741149
47 47 a, c, g dbSNP:138573028
78 78 a, g dbSNP:570304277
109 109 a, g dbSNP:550393268
120 120 g, t dbSNP:775026517
134 134 g, t dbSNP:540162615
151 151 g, t dbSNP:536323085
159 159 c, t dbSNP:771732248
177 177 c, g dbSNP:571141893
205 205 g, t dbSNP:1130214
211 211 c, t dbSNP:528047226
233 233 a, g dbSNP:10138227
238 238 c, t dbSNP:771481743
301 301 c, g dbSNP:548761391
311 311 a, g dbSNP:190916256
333 333 a, g dbSNP:778182689
362 362 a, g dbSNP:187239247
388 388 c, t dbSNP:756176055
389 389 c, t dbSNP:147187121
397 397 a, t dbSNP:748239540
398 398 a, c dbSNP:577532120
407 407 c, t dbSNP:564014530
463 463 a, g dbSNP:541421272
490 490 a, c dbSNP:371297714
493 493 a, g dbSNP:11555428
501 501 c, t dbSNP:753859473
502 502 a, g dbSNP:548942912
507 507 a, g dbSNP:370260488
508 508 c, t dbSNP:757627335
509 509 a, g dbSNP:34790457
510 510 c, g dbSNP:34757714
514 514 c, t dbSNP:763526322
515 515 -, agcc dbSNP:757126193
521 521 a, g dbSNP:752773454
522 522 c, g dbSNP:765349412
524 524 c, g dbSNP:373561553
525 525 c, t dbSNP:369911858
526 526 a, g dbSNP:181751951
527 527 c, t dbSNP:761545130
528 528 a, c, g dbSNP:768619756
534 534 a, g dbSNP:749225421
536 536 c, t dbSNP:779484182
538 538 c, t dbSNP:769337829
539 539 a, c, g dbSNP:780836176
544 544 c, t dbSNP:757729900
547 547 c, g, t dbSNP:28730786
548 548 a, g dbSNP:113993465
560 560 c, t dbSNP:371534192
561 561 a, g dbSNP:140532443
563 563 c, t dbSNP:56217512
564 564 a, g dbSNP:754031503
567 567 a, g dbSNP:766546254
587 587 a, g dbSNP:760853972
598 598 a, g dbSNP:368797346
603 603 a, g dbSNP:121434592
603 603 -, g dbSNP:34409589
605 605 a, g dbSNP:779114873
627 627 c, t dbSNP:397514644
632 632 c, t dbSNP:769253794
635 635 c, t dbSNP:749781543
646 646 a, g dbSNP:780173607
650 650 c, g, t dbSNP:201636005
655 655 a, c dbSNP:750653493
660 660 a, g dbSNP:781339141
661 661 c, t dbSNP:758157217
668 668 c, t dbSNP:752588225
669 669 a, g dbSNP:764931115
674 674 a, g dbSNP:370287382
675 675 c, t dbSNP:753765116
676 676 a, g dbSNP:766000895
680 680 a, g dbSNP:375645311
681 681 c, t dbSNP:11555436
692 692 a, c dbSNP:146875699
695 695 a, g dbSNP:532268608
696 696 c, t dbSNP:374093099
697 697 a, g dbSNP:774836044
703 703 c, t dbSNP:768898540
707 707 c, t dbSNP:749796064
710 710 c, t dbSNP:780477141
725 725 a, g dbSNP:769881484
728 728 a, g dbSNP:560243859
734 734 c, t dbSNP:767058127
740 740 a, g dbSNP:756659788
748 748 c, t dbSNP:751243134
749 749 a, g dbSNP:768384015
753 753 c, t dbSNP:762559261
759 759 c, t dbSNP:775892860
760 760 a, g dbSNP:183989506
768 768 a, g dbSNP:760044481
779 779 c, g dbSNP:587778019
781 781 a, g dbSNP:771065764
791 791 a, g dbSNP:747020429
800 800 c, t dbSNP:773311402
810 810 c, t dbSNP:772473246
811 811 a, g dbSNP:773971502
815 815 c, t dbSNP:748365698
821 821 c, t dbSNP:142646145
840 840 c, t dbSNP:760536822
848 848 a, g dbSNP:766755485
855 855 a, g dbSNP:761151169
857 857 c, t dbSNP:750890213
869 869 c, t dbSNP:767715614
872 872 c, g dbSNP:761930839
877 877 a, c dbSNP:774745066
879 879 a, g dbSNP:768800433
899 899 a, g dbSNP:147664316
903 903 -, gag dbSNP:768025881
903 903 a, g dbSNP:776345122
915 915 c, t dbSNP:369198922
919 919 c, t dbSNP:746934495
920 920 a, g dbSNP:777639707
928 928 c, t dbSNP:759031755
941 941 a, g dbSNP:747700076
960 960 a, g dbSNP:778376616
981 981 c, t dbSNP:142843688
984 984 c, t dbSNP:375990114
986 986 c, t dbSNP:17846825
992 992 c, g, t dbSNP:145780379
994 994 c, t dbSNP:758456890
998 998 c, t dbSNP:752894366
1007 1007 a, g dbSNP:765537155
1011 1011 c, t dbSNP:149803079
1023 1023 c, g dbSNP:568870136
1025 1025 a, c, t dbSNP:767298307
1037 1037 c, t dbSNP:139904675
1054 1054 c, t dbSNP:11555433
1064 1064 g, t dbSNP:774284624
1066 1066 c, t dbSNP:768070795
1069 1069 c, t dbSNP:748789094
1079 1079 c, t dbSNP:774828129
1087 1087 c, t dbSNP:769619023
1108 1108 g, t dbSNP:745500951
1112 1112 c, t dbSNP:34670300
1115 1115 g, t dbSNP:757639898
1116 1116 a, g dbSNP:144128670
1124 1124 c, t dbSNP:780207480
1132 1132 c, t dbSNP:756293014
1133 1133 c, g dbSNP:139453090
1141 1141 a, t dbSNP:751416672
1145 1145 c, t dbSNP:763782135
1154 1154 c, t dbSNP:61761191
1155 1155 a, g dbSNP:774360915
1158 1158 c, t dbSNP:2230506
1160 1160 a, g dbSNP:759079296
1169 1169 c, t dbSNP:368770666
1184 1184 c, g dbSNP:776250457
1196 1196 a, g dbSNP:765986469
1198 1198 a, g dbSNP:760384228
1199 1199 c, t dbSNP:772685079
1201 1201 -, ct dbSNP:770828544
1214 1214 c, t dbSNP:141168205
1215 1215 c, g dbSNP:762040581
1218 1218 c, t dbSNP:551254461
1219 1219 a, g dbSNP:369698909
1223 1223 c, g dbSNP:376531915
1234 1234 c, t dbSNP:775612804
1241 1241 c, t dbSNP:769696222
1247 1247 c, t dbSNP:767346459
1248 1248 a, g dbSNP:781232725
1250 1250 a, g dbSNP:201006619
1253 1253 c, t dbSNP:371786809
1254 1254 a, g dbSNP:778838358
1257 1257 c, g dbSNP:770980034
1259 1259 g, t dbSNP:769090254
1273 1273 c, g dbSNP:773520823
1275 1275 c, t dbSNP:768606668
1280 1280 a, g dbSNP:1130233
1290 1290 g, t dbSNP:779874420
1292 1292 c, t dbSNP:368667384
1300 1300 a, g dbSNP:745809388
1305 1305 c, t dbSNP:780571834
1306 1306 a, g dbSNP:756697784
1312 1312 a, g dbSNP:751232562
1317 1317 a, g dbSNP:375395037
1335 1335 c, g dbSNP:758025607
1337 1337 a, g dbSNP:143933508
1340 1340 c, g dbSNP:371467719
1343 1343 c, t dbSNP:759966382
1344 1344 c, t dbSNP:754317207
1351 1351 c, t dbSNP:549083521
1352 1352 a, g dbSNP:367635775
1361 1361 c, t dbSNP:201044857
1364 1364 a, g dbSNP:2230508
1373 1373 c, g dbSNP:762108714
1382 1382 a, g dbSNP:775212616
1409 1409 c, t dbSNP:149484260
1416 1416 a, g dbSNP:767699129
1430 1430 c, t dbSNP:761942512
1433 1433 c, t dbSNP:139200289
1439 1439 a, g dbSNP:373838774
1460 1460 c, t dbSNP:746001369
1484 1484 c, t dbSNP:146847196
1493 1493 c, t dbSNP:776378715
1502 1502 a, g dbSNP:751320716
1503 1503 -, g dbSNP:780293460
1505 1505 a, c dbSNP:17846831
1508 1508 c, t dbSNP:746944273
1510 1510 a, g dbSNP:12881616
1525 1525 a, g dbSNP:746397537
1527 1527 a, g dbSNP:781749630
1532 1532 c, t dbSNP:55874341
1541 1541 a, g dbSNP:751709437
1544 1544 a, g dbSNP:777711698
1559 1559 c, g dbSNP:758651475
1574 1574 c, t dbSNP:144088506
1586 1586 c, t dbSNP:56289559
1604 1604 c, t dbSNP:760425021
1607 1607 c, t dbSNP:376942361
1613 1613 c, t dbSNP:767544462
1616 1616 c, t dbSNP:761646283
1624 1624 c, t dbSNP:11555432
1641 1641 a, g dbSNP:372502847
1646 1646 a, g dbSNP:773975370
1653 1653 a, c, t dbSNP:762705090
1654 1654 a, g dbSNP:369520527
1661 1661 a, g dbSNP:150399268
1662 1662 c, t dbSNP:549370342
1663 1663 a, g dbSNP:746272761
1666 1666 c, t dbSNP:781388586
1667 1667 a, c, g dbSNP:747408700
1676 1676 c, t dbSNP:374865919
1677 1677 a, g dbSNP:373253729
1688 1688 c, t dbSNP:369527283
1700 1700 a, c, g dbSNP:755174756
1703 1703 a, c, g dbSNP:767207905
1710 1710 -, aag dbSNP:758624702
1712 1712 a, g dbSNP:761810870
1716 1716 a, c dbSNP:11555431
1733 1733 c, t dbSNP:11555434
1743 1743 a, g dbSNP:531850885
1744 1744 a, c dbSNP:766798551
1748 1748 c, t dbSNP:151254374
1749 1749 a, g dbSNP:188580689
1752 1752 a, g dbSNP:773607483
1754 1754 a, g dbSNP:772629378
1760 1760 c, t dbSNP:748654766
1763 1763 a, g dbSNP:774414969
1768 1768 a, c dbSNP:768660759
1770 1770 c, t dbSNP:749544983
1771 1771 a, g dbSNP:143266084
1781 1781 c, t dbSNP:141303557
1782 1782 a, g dbSNP:146483593
1787 1787 c, t dbSNP:375901448
1797 1797 c, t dbSNP:777696700
1799 1799 c, t dbSNP:758476416
1805 1805 c, t dbSNP:139297659
1806 1806 a, g dbSNP:764863282
1810 1810 a, g dbSNP:754500025
1812 1812 a, g dbSNP:753360468
1823 1823 a, g dbSNP:201227909
1843 1843 c, t dbSNP:749186394
1844 1844 a, g dbSNP:779447501
1846 1846 c, t dbSNP:755597789
1847 1847 a, g dbSNP:750104452
1857 1857 a, c dbSNP:397514645
1859 1859 c, t dbSNP:780860941
1883 1883 a, g dbSNP:376893212
1892 1892 g, t dbSNP:751976958
1893 1893 a, g dbSNP:764514218
1898 1898 c, t dbSNP:758833789
1909 1909 a, c dbSNP:753190812
1913 1913 c, g dbSNP:765264778
1915 1915 a, g dbSNP:759702315
1918 1918 a, g dbSNP:754040453
1925 1925 c, g dbSNP:759902535
1927 1927 a, c, t dbSNP:587778018
1934 1934 c, t dbSNP:139394285
1935 1935 g, t dbSNP:11555435
1937 1937 a, g dbSNP:763831402
1940 1940 c, t dbSNP:762849643
1943 1943 a, c, t dbSNP:144112075
1944 1944 a, g dbSNP:745803788
1947 1947 c, g, t dbSNP:770370100
1948 1948 a, g dbSNP:113547523
1970 1970 c, t dbSNP:184263655
1975 1975 a, c dbSNP:757963528
1979 1979 a, g dbSNP:748603087
1985 1985 c, t dbSNP:200213561
1990 1990 c, t dbSNP:201291259
1991 1991 a, g dbSNP:555187157
2000 2000 c, t dbSNP:780299611
2001 2001 a, g dbSNP:756226133
2003 2003 c, t dbSNP:200508405
2004 2004 a, g dbSNP:192237287
2005 2005 a, g, t dbSNP:111793056
2007 2007 a, g dbSNP:752606488
2013 2013 c, t dbSNP:377339529
2014 2014 a, g dbSNP:373329736
2020 2020 c, t dbSNP:375585585
2021 2021 a, c, g dbSNP:370987171
2032 2032 a, g dbSNP:558891046
2034 2034 a, g dbSNP:539258432
2035 2035 -, a dbSNP:761112590
2044 2044 c, t dbSNP:368920620
2045 2045 a, g dbSNP:573353277
2046 2046 a, g dbSNP:11555430
2083 2083 c, t dbSNP:553657544
2107 2107 c, t dbSNP:17846821
2118 2118 c, t dbSNP:17846826
2133 2133 a, g dbSNP:548181379
2136 2136 c, t dbSNP:537945247
2167 2167 a, g dbSNP:373002859
2227 2227 a, c, t dbSNP:112281303
2246 2246 c, g dbSNP:61761249
2265 2265 c, t dbSNP:34558581
2272 2272 a, g dbSNP:532385857
2298 2298 c, t dbSNP:41307094
2310 2310 c, t dbSNP:770697761
2320 2320 a, c, g dbSNP:1130245
2326 2326 a, g dbSNP:137981458
2335 2335 a, c dbSNP:560947797
2341 2341 c, t dbSNP:544158185
2351 2351 -, tgtc dbSNP:575655519
2363 2363 a, g dbSNP:575222918
2365 2365 a, c dbSNP:34865723
2376 2376 -, tgtc dbSNP:756938164
2376 2376 a, t dbSNP:113613978
2388 2388 g, t dbSNP:35416681
2397 2397 a, t dbSNP:573418247
2398 2398 a, c dbSNP:553256558
2433 2433 a, g dbSNP:765101207
2434 2434 c, t dbSNP:539274348
2441 2441 c, t dbSNP:754575126
2493 2493 a, g dbSNP:574337857
2500 2500 a, c dbSNP:554616284
2517 2517 c, t dbSNP:537882189
2519 2519 a, t dbSNP:568759365
2531 2531 c, t dbSNP:570600044
2542 2542 g, t dbSNP:1804268
2551 2551 c, t dbSNP:1130295
2589 2589 a, g dbSNP:751165631
2611 2611 a, g dbSNP:780314185
2666 2666 a, g dbSNP:758739510
2672 2672 -, g dbSNP:566232803
2701 2701 a, g dbSNP:1130443
2732 2732 a, g dbSNP:552278695
2761 2761 c, t dbSNP:112114610
2762 2762 a, g dbSNP:566677791
2767 2767 -, acttgaccttttcg dbSNP:55707948
2775 2775 c, t dbSNP:559789852
2783 2783 -, ttgaccttttcgac, ttgacctttttgac dbSNP:3840005
2783 2783 c, g, t dbSNP:762049014
2784 2784 a, g dbSNP:186886987
2792 2792 c, t dbSNP:769089409
2796 2796 c, t dbSNP:749839506
2802 2802 c, t dbSNP:753950090
2805 2805 c, t dbSNP:764172888
2815 2815 c, t dbSNP:3803305
2817 2817 c, t dbSNP:567261290
2850 2850 c, t dbSNP:17846828
2851 2851 c, g dbSNP:58565216
2857 2857 a, g dbSNP:17846829
2871 2871 c, t dbSNP:183360200
2877 2877 c, t dbSNP:17102385
2883 2883 c, t dbSNP:528550066
2884 2884 a, g dbSNP:140320743
2886 2886 c, t dbSNP:773176237
2888 2888 c, t dbSNP:190605178
2893 2893 c, t dbSNP:11555429
2937 2937 a, g dbSNP:769719936
2966 2966 c, t dbSNP:1130526

Target ORF information:

RefSeq Version NM_005163
Organism Homo sapiens (human)
Definition Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005163

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Accession Version NM_001014431.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414251.1 and BC000479.2. Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]. Transcript Variant: This variant (3) lacks the 5' exon, but has an upstream alternate 5' exon, as compared to variant 1. Variants 1 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000479.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001014431.1
CDS341..1783
Misc Feature(1)302..304(+)
Misc Feature(2)350..673(+)
Misc Feature(3)380..598(+)
Misc Feature(4)380..397(+)
Misc Feature(5)380..382(+)
Misc Feature(6)398..400(+)
Misc Feature(7)407..415(+)
Misc Feature(8)710..1777(+)
Misc Feature(9)710..712(+)
Misc Feature(10)710..712(+)
Misc Feature(11)716..718(+)
Misc Feature(12)716..718(+)
Misc Feature(13)725..727(+)
Misc Feature(14)725..727(+)
Misc Feature(15)788..1564(+)
Misc Feature(16)806..1666(+)
Misc Feature(17)806..1654(+)
Misc Feature(18)818..1666(+)
Misc Feature(19)866..868(+)
Misc Feature(20)1022..1030(+)
Misc Feature(21)1211..1282(+)
Misc Feature(22)1262..1264(+)
Misc Feature(23)1262..1264(+)
Misc Feature(24)1262..1264(+)
Misc Feature(25)1262..1264(+)
Misc Feature(26)1262..1264(+)
Misc Feature(27)1262..1264(+)
Misc Feature(28)1283..1285(+)
Misc Feature(29)1316..1318(+)
Misc Feature(30)1688..1690(+)
Misc Feature(31)1688..1690(+)
Misc Feature(32)1724..1726(+)
Misc Feature(33)1745..1762(+)
Misc Feature(34)1757..1759(+)
Misc Feature(35)1757..1759(+)
Misc Feature(36)1757..1759(+)
Misc Feature(37)1757..1759(+)
Misc Feature(38)1757..1759(+)
Misc Feature(39)1757..1759(+)
Misc Feature(40)1757..1759(+)
Misc Feature(41)1760..1762(+)
Misc Feature(42)1760..1762(+)
Exon (1)1..261
Gene:AKT1
Gene Synonym:
Exon (2)262..386
Gene:AKT1
Gene Synonym:
Exon (3)387..515
Gene:AKT1
Gene Synonym:
Exon (4)516..627
Gene:AKT1
Gene Synonym:
Exon (5)628..775
Gene:AKT1
Gene Synonym:
Exon (6)776..907
Gene:AKT1
Gene Synonym:
Exon (7)908..973
Gene:AKT1
Gene Synonym:
Exon (8)974..1042
Gene:AKT1
Gene Synonym:
Exon (9)1043..1168
Gene:AKT1
Gene Synonym:
Exon (10)1169..1297
Gene:AKT1
Gene Synonym:
Exon (11)1298..1512
Gene:AKT1
Gene Synonym:
Exon (12)1513..1600
Gene:AKT1
Gene Synonym:
Exon (13)1601..1703
Gene:AKT1
Gene Synonym:
Exon (14)1704..2775
Gene:AKT1
Gene Synonym:
Translation
Position Chain Variation Link
39 39 c, g dbSNP:3001372
39 39 c, g dbSNP:3002448
40 40 c, g dbSNP:575429386
276 276 a, c dbSNP:371297714
279 279 a, g dbSNP:11555428
287 287 c, t dbSNP:753859473
288 288 a, g dbSNP:548942912
293 293 a, g dbSNP:370260488
294 294 c, t dbSNP:757627335
295 295 a, g dbSNP:34790457
296 296 c, g dbSNP:34757714
300 300 c, t dbSNP:763526322
301 301 -, agcc dbSNP:757126193
307 307 a, g dbSNP:752773454
308 308 c, g dbSNP:765349412
310 310 c, g dbSNP:373561553
311 311 c, t dbSNP:369911858
312 312 a, g dbSNP:181751951
313 313 c, t dbSNP:761545130
314 314 a, c, g dbSNP:768619756
320