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AKT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol AKT1
Entrez Gene ID 207
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q32.32

Summary The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]. lac of sum
Disorder MIM:

164730

Disorder Html: Breast cancer, somatic, 114480 (3); Colorectal cancer, somatic,

mRNA and Protein(s)

mRNA Protein Name
XM_011536543 XP_011534845 RAC-alpha serine/threonine-protein kinase isoform X1
XM_005267401 XP_005267458 RAC-alpha serine/threonine-protein kinase isoform X1
XM_011536544 XP_011534846 RAC-alpha serine/threonine-protein kinase isoform X2
NM_005163 NP_005154 RAC-alpha serine/threonine-protein kinase
NM_001014431 NP_001014431 RAC-alpha serine/threonine-protein kinase
NM_001014432 NP_001014432 RAC-alpha serine/threonine-protein kinase



Homo sapiens (human) AKT1 NP_001014431.1
Pan troglodytes (chimpanzee) AKT1 XP_001143158.2
Macaca mulatta (Rhesus monkey) AKT1 XP_001085265.2
Canis lupus familiaris (dog) AKT1 XP_548000.3
Bos taurus (cattle) AKT1 NP_776411.1
Mus musculus (house mouse) Akt1 NP_033782.1
Rattus norvegicus (Norway rat) Akt1 NP_150233.1
Gallus gallus (chicken) AKT1 NP_990386.1
Danio rerio (zebrafish) LOC100331515 XP_003200336.2
Caenorhabditis elegans akt-1 NP_001023646.1
Caenorhabditis elegans akt-2 NP_510357.3
Xenopus (Silurana) tropicalis (western clawed frog) akt1 XP_004917249.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AKT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25680
XM_011536543 PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu25680
XM_005267401 PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu56223 XM_011536544 PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25680
NM_005163 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu25680
NM_001014431 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu25680
NM_001014432 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25680
Clone ID Related Accession (Same CDS sequence) NM_001014431 , NM_001014432 , NM_005163 , XM_005267401 , XM_011536543
Accession Version XM_011536543.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534845.1
CDS668..2110
Misc Feature(1)677..1000(+)
Misc Feature(2)707..925(+)
Misc Feature(3)1037..2104(+)
Misc Feature(4)1115..1891(+)
Misc Feature(5)1133..1993(+)
Misc Feature(6)1133..1981(+)
Misc Feature(7)1145..1993(+)
Misc Feature(8)1538..1609(+)
Misc Feature(9)2015..2017(+)
Misc Feature(10)2072..2089(+)
Translation

Target ORF information:

RefSeq Version XM_011536543
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536543

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Clone ID Related Accession (Same CDS sequence) NM_001014431 , NM_001014432 , NM_005163 , XM_005267401 , XM_011536543
Accession Version XM_005267401.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005267458.1
CDS490..1932
Misc Feature(1)499..822(+)
Misc Feature(2)529..747(+)
Misc Feature(3)859..1926(+)
Misc Feature(4)937..1713(+)
Misc Feature(5)955..1815(+)
Misc Feature(6)955..1803(+)
Misc Feature(7)967..1815(+)
Misc Feature(8)1360..1431(+)
Misc Feature(9)1837..1839(+)
Misc Feature(10)1894..1911(+)
Translation

Target ORF information:

RefSeq Version XM_005267401
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005267401

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56223
Clone ID Related Accession (Same CDS sequence) XM_011536544
Accession Version XM_011536544.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
GGACCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534846.1
CDS1482..2750
Misc Feature(1)1491..1814(+)
Misc Feature(2)1521..1739(+)
Misc Feature(3)1851..2741(+)
Misc Feature(4)1929..2705(+)
Misc Feature(5)1947..2357(+)
Translation

Target ORF information:

RefSeq Version XM_011536544
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536544

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
GGACCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Clone ID Related Accession (Same CDS sequence) NM_001014431 , NM_001014432 , NM_005163 , XM_005267401 , XM_011536543
Accession Version NM_005163.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122894.1 and BC000479.2. This sequence is a reference standard in the RefSeqGene project. On Apr 6, 2005 this sequence version replaced gi:4885060. Transcript Variant: This variant (1) is the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122894.1, M63167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005154.2
CDS555..1997
Misc Feature(1)516..518(+)
Misc Feature(2)564..887(+)
Misc Feature(3)594..812(+)
Misc Feature(4)594..611(+)
Misc Feature(5)594..596(+)
Misc Feature(6)612..614(+)
Misc Feature(7)621..629(+)
Misc Feature(8)924..1991(+)
Misc Feature(9)924..926(+)
Misc Feature(10)924..926(+)
Misc Feature(11)930..932(+)
Misc Feature(12)930..932(+)
Misc Feature(13)939..941(+)
Misc Feature(14)939..941(+)
Misc Feature(15)1002..1778(+)
Misc Feature(16)1020..1880(+)
Misc Feature(17)1020..1868(+)
Misc Feature(18)1032..1880(+)
Misc Feature(19)1080..1082(+)
Misc Feature(20)1236..1244(+)
Misc Feature(21)1425..1496(+)
Misc Feature(22)1476..1478(+)
Misc Feature(23)1476..1478(+)
Misc Feature(24)1476..1478(+)
Misc Feature(25)1476..1478(+)
Misc Feature(26)1476..1478(+)
Misc Feature(27)1476..1478(+)
Misc Feature(28)1497..1499(+)
Misc Feature(29)1530..1532(+)
Misc Feature(30)1902..1904(+)
Misc Feature(31)1902..1904(+)
Misc Feature(32)1938..1940(+)
Misc Feature(33)1959..1976(+)
Misc Feature(34)1971..1973(+)
Misc Feature(35)1971..1973(+)
Misc Feature(36)1971..1973(+)
Misc Feature(37)1971..1973(+)
Misc Feature(38)1971..1973(+)
Misc Feature(39)1971..1973(+)
Misc Feature(40)1971..1973(+)
Misc Feature(41)1974..1976(+)
Misc Feature(42)1974..1976(+)
Exon (1)1..475
Gene:AKT1
Gene Synonym:
Exon (2)476..600
Gene:AKT1
Gene Synonym:
Exon (3)601..729
Gene:AKT1
Gene Synonym:
Exon (4)730..841
Gene:AKT1
Gene Synonym:
Exon (5)842..989
Gene:AKT1
Gene Synonym:
Exon (6)990..1121
Gene:AKT1
Gene Synonym:
Exon (7)1122..1187
Gene:AKT1
Gene Synonym:
Exon (8)1188..1256
Gene:AKT1
Gene Synonym:
Exon (9)1257..1382
Gene:AKT1
Gene Synonym:
Exon (10)1383..1511
Gene:AKT1
Gene Synonym:
Exon (11)1512..1726
Gene:AKT1
Gene Synonym:
Exon (12)1727..1814
Gene:AKT1
Gene Synonym:
Exon (13)1815..1917
Gene:AKT1
Gene Synonym:
Exon (14)1918..2989
Gene:AKT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005163
Organism Homo sapiens (human)
Definition Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005163

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Clone ID Related Accession (Same CDS sequence) NM_001014431 , NM_001014432 , NM_005163 , XM_005267401 , XM_011536543
Accession Version NM_001014431.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414251.1 and BC000479.2. Transcript Variant: This variant (3) lacks the 5' exon, but has an upstream alternate 5' exon, as compared to variant 1. Variants 1 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000479.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001014431.1
CDS341..1783
Misc Feature(1)302..304(+)
Misc Feature(2)350..673(+)
Misc Feature(3)380..598(+)
Misc Feature(4)380..397(+)
Misc Feature(5)380..382(+)
Misc Feature(6)398..400(+)
Misc Feature(7)407..415(+)
Misc Feature(8)710..1777(+)
Misc Feature(9)710..712(+)
Misc Feature(10)710..712(+)
Misc Feature(11)716..718(+)
Misc Feature(12)716..718(+)
Misc Feature(13)725..727(+)
Misc Feature(14)725..727(+)
Misc Feature(15)788..1564(+)
Misc Feature(16)806..1666(+)
Misc Feature(17)806..1654(+)
Misc Feature(18)818..1666(+)
Misc Feature(19)866..868(+)
Misc Feature(20)1022..1030(+)
Misc Feature(21)1211..1282(+)
Misc Feature(22)1262..1264(+)
Misc Feature(23)1262..1264(+)
Misc Feature(24)1262..1264(+)
Misc Feature(25)1262..1264(+)
Misc Feature(26)1262..1264(+)
Misc Feature(27)1262..1264(+)
Misc Feature(28)1283..1285(+)
Misc Feature(29)1316..1318(+)
Misc Feature(30)1688..1690(+)
Misc Feature(31)1688..1690(+)
Misc Feature(32)1724..1726(+)
Misc Feature(33)1745..1762(+)
Misc Feature(34)1757..1759(+)
Misc Feature(35)1757..1759(+)
Misc Feature(36)1757..1759(+)
Misc Feature(37)1757..1759(+)
Misc Feature(38)1757..1759(+)
Misc Feature(39)1757..1759(+)
Misc Feature(40)1757..1759(+)
Misc Feature(41)1760..1762(+)
Misc Feature(42)1760..1762(+)
Exon (1)1..261
Gene:AKT1
Gene Synonym:
Exon (2)262..386
Gene:AKT1
Gene Synonym:
Exon (3)387..515
Gene:AKT1
Gene Synonym:
Exon (4)516..627
Gene:AKT1
Gene Synonym:
Exon (5)628..775
Gene:AKT1
Gene Synonym:
Exon (6)776..907
Gene:AKT1
Gene Synonym:
Exon (7)908..973
Gene:AKT1
Gene Synonym:
Exon (8)974..1042
Gene:AKT1
Gene Synonym:
Exon (9)1043..1168
Gene:AKT1
Gene Synonym:
Exon (10)1169..1297
Gene:AKT1
Gene Synonym:
Exon (11)1298..1512
Gene:AKT1
Gene Synonym:
Exon (12)1513..1600
Gene:AKT1
Gene Synonym:
Exon (13)1601..1703
Gene:AKT1
Gene Synonym:
Exon (14)1704..2775
Gene:AKT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001014431
Organism Homo sapiens (human)
Definition Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001014431

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25680
Clone ID Related Accession (Same CDS sequence) NM_001014431 , NM_001014432 , NM_005163 , XM_005267401 , XM_011536543
Accession Version NM_001014432.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product RAC-alpha serine/threonine-protein kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN414251.1, BC084538.1 and BC000479.2. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) lacks the 5' segment, but has an upstream alternate 5' exon, as compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC084538.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001014432.1
CDS425..1867
Misc Feature(1)386..388(+)
Misc Feature(2)434..757(+)
Misc Feature(3)464..682(+)
Misc Feature(4)464..481(+)
Misc Feature(5)464..466(+)
Misc Feature(6)482..484(+)
Misc Feature(7)491..499(+)
Misc Feature(8)794..1861(+)
Misc Feature(9)794..796(+)
Misc Feature(10)794..796(+)
Misc Feature(11)800..802(+)
Misc Feature(12)800..802(+)
Misc Feature(13)809..811(+)
Misc Feature(14)809..811(+)
Misc Feature(15)872..1648(+)
Misc Feature(16)890..1750(+)
Misc Feature(17)890..1738(+)
Misc Feature(18)902..1750(+)
Misc Feature(19)950..952(+)
Misc Feature(20)1106..1114(+)
Misc Feature(21)1295..1366(+)
Misc Feature(22)1346..1348(+)
Misc Feature(23)1346..1348(+)
Misc Feature(24)1346..1348(+)
Misc Feature(25)1346..1348(+)
Misc Feature(26)1346..1348(+)
Misc Feature(27)1346..1348(+)
Misc Feature(28)1367..1369(+)
Misc Feature(29)1400..1402(+)
Misc Feature(30)1772..1774(+)
Misc Feature(31)1772..1774(+)
Misc Feature(32)1808..1810(+)
Misc Feature(33)1829..1846(+)
Misc Feature(34)1841..1843(+)
Misc Feature(35)1841..1843(+)
Misc Feature(36)1841..1843(+)
Misc Feature(37)1841..1843(+)
Misc Feature(38)1841..1843(+)
Misc Feature(39)1841..1843(+)
Misc Feature(40)1841..1843(+)
Misc Feature(41)1844..1846(+)
Misc Feature(42)1844..1846(+)
Exon (1)1..261
Gene:AKT1
Gene Synonym:
Exon (2)262..345
Gene:AKT1
Gene Synonym:
Exon (3)346..470
Gene:AKT1
Gene Synonym:
Exon (4)471..599
Gene:AKT1
Gene Synonym:
Exon (5)600..711
Gene:AKT1
Gene Synonym:
Exon (6)712..859
Gene:AKT1
Gene Synonym:
Exon (7)860..991
Gene:AKT1
Gene Synonym:
Exon (8)992..1057
Gene:AKT1
Gene Synonym:
Exon (9)1058..1126
Gene:AKT1
Gene Synonym:
Exon (10)1127..1252
Gene:AKT1
Gene Synonym:
Exon (11)1253..1381
Gene:AKT1
Gene Synonym:
Exon (12)1382..1596
Gene:AKT1
Gene Synonym:
Exon (13)1597..1684
Gene:AKT1
Gene Synonym:
Exon (14)1685..1787
Gene:AKT1
Gene Synonym:
Exon (15)1788..2859
Gene:AKT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001014432
Organism Homo sapiens (human)
Definition Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001014432

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCGACG TGGCTATTGT GAAGGAGGGT TGGCTGCACA AACGAGGGGA GTACATCAAG 
ACCTGGCGGC CACGCTACTT CCTCCTCAAG AATGATGGCA CCTTCATTGG CTACAAGGAG
CGGCCGCAGG ATGTGGACCA ACGTGAGGCT CCCCTCAACA ACTTCTCTGT GGCGCAGTGC
CAGCTGATGA AGACGGAGCG GCCCCGGCCC AACACCTTCA TCATCCGCTG CCTGCAGTGG
ACCACTGTCA TCGAACGCAC CTTCCATGTG GAGACTCCTG AGGAGCGGGA GGAGTGGACA
ACCGCCATCC AGACTGTGGC TGACGGCCTC AAGAAGCAGG AGGAGGAGGA GATGGACTTC
CGGTCGGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG
CCCAAGCACC GCGTGACCAT GAACGAGTTT GAGTACCTGA AGCTGCTGGG CAAGGGCACT
TTCGGCAAGG TGATCCTGGT GAAGGAGAAG GCCACAGGCC GCTACTACGC CATGAAGATC
CTCAAGAAGG AAGTCATCGT GGCCAAGGAC GAGGTGGCCC ACACACTCAC CGAGAACCGC
GTCCTGCAGA ACTCCAGGCA CCCCTTCCTC ACAGCCCTGA AGTACTCTTT CCAGACCCAC
GACCGCCTCT GCTTTGTCAT GGAGTACGCC AACGGGGGCG AGCTGTTCTT CCACCTGTCC
CGGGAGCGTG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GCGCTGAGAT TGTGTCAGCC
CTGGACTACC TGCACTCGGA GAAGAACGTG GTGTACCGGG ACCTCAAGCT GGAGAACCTC
ATGCTGGACA AGGACGGGCA CATTAAGATC ACAGACTTCG GGCTGTGCAA GGAGGGGATC
AAGGACGGTG CCACCATGAA GACCTTTTGC GGCACACCTG AGTACCTGGC CCCCGAGGTG
CTGGAGGACA ATGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAC
GAGATGATGT GCGGTCGCCT GCCCTTCTAC AACCAGGACC ATGAGAAGCT TTTTGAGCTC
ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACGCTTGGTC CCGAGGCCAA GTCCTTGCTT
TCAGGGCTGC TCAAGAAGGA CCCCAAGCAG AGGCTTGGCG GGGGCTCCGA GGACGCCAAG
GAGATCATGC AGCATCGCTT CTTTGCCGGT ATCGTGTGGC AGCACGTGTA CGAGAAGAAG
CTCAGCCCAC CCTTCAAGCC CCAGGTCACG TCGGAGACTG ACACCAGGTA TTTTGATGAG
GAGTTCACGG CCCAGATGAT CACCATCACA CCACCTGACC AAGATGACAG CATGGAGTGT
GTGGACAGCG AGCGCAGGCC CCACTTCCCC CAGTTCTCCT ACTCGGCCAG CGGCACGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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