×

ERCC3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ERCC3
Entrez Gene ID 2071
Full Name excision repair cross-complementation group 3
Synonyms BTF2, GTF2H, RAD25, TFIIH, TTD2, XPB
General protein information
Preferred Names
TFIIH basal transcription factor complex helicase XPB subunit
Names
TFIIH basal transcription factor complex helicase XPB subunit
BTF2 p89
TFIIH p89
TFIIH 89 kDa subunit
DNA excision repair protein ERCC-3
DNA repair protein complementing XP-B cells
basic transcription factor 2 89 kDa subunit
xeroderma pigmentosum, complementation group B
xeroderma pigmentosum group B-complementing protein
TFIIH basal transcription factor complex 89 kDa subunit
NP_000113.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q21

Summary This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]. lac of sum
Disorder MIM:

133510

Disorder Html: Xeroderma pigmentosum, group B, 610651 (3); Trichothiodystrophy,

mRNA and Protein(s)

mRNA Protein Name
XM_011510794 XP_011509096 TFIIH basal transcription factor complex helicase XPB subunit isoform X1
XM_011510795 XP_011509097 TFIIH basal transcription factor complex helicase XPB subunit isoform X2
NM_001303416 NP_001290345 TFIIH basal transcription factor complex helicase XPB subunit isoform b
NM_001303418 NP_001290347 TFIIH basal transcription factor complex helicase XPB subunit isoform b
NM_000122 NP_000113 TFIIH basal transcription factor complex helicase XPB subunit isoform a



Homo sapiens (human) ERCC3 NP_000113.1
Pan troglodytes (chimpanzee) ERCC3 XP_525907.3
Macaca mulatta (Rhesus monkey) ERCC3 XP_001086722.1
Canis lupus familiaris (dog) ERCC3 XP_533314.2
Bos taurus (cattle) ERCC3 NP_001039453.1
Mus musculus (house mouse) Ercc3 NP_598419.1
Rattus norvegicus (Norway rat) Ercc3 NP_001026814.1
Gallus gallus (chicken) ERCC3 NP_001006523.1
Danio rerio (zebrafish) ercc3 NP_963876.1
Drosophila melanogaster (fruit fly) hay NP_524020.2
Caenorhabditis elegans Y66D12A.15 NP_499487.1
Arabidopsis thaliana (thale cress) XPB1 NP_568592.1
Arabidopsis thaliana (thale cress) XPB2 NP_568591.1
Xenopus (Silurana) tropicalis (western clawed frog) ercc3 NP_001123812.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ERCC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERCC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu57567 XM_011510794 PREDICTED: Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu57568 XM_011510795 PREDICTED: Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu35980 NM_001303416 Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu35980 NM_001303418 Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu25443
NM_000122 Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57567
Clone ID Related Accession (Same CDS sequence) XM_011510794
Accession Version XM_011510794.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCCAC
CTACCTCTCT CACAGATTTC TAAGACTGCT GAAAGCAGTG GTGGGCCCTC CACTTCCCGA
GTGACAGATC CACAGGGTAA ATCTGACATC CCCATGGACC TGTTTGACTT CTATGAGCAA
ATGGACAAGG ATGAAGAAGA AGAAGAAGAG ACACAGACAG TGTCTTTTGA AGTCAAGCAG
GAAATGATTG AGGAACTCCA GAAACGTTGC ATCCACCTGG AGTACCCTCT GTTGGCAGAA
TATGACTTCC GGAATGATTC TGTCAACCCT GATATCAACA TTGACCTAAA GCCCACAGCT
GTCCTCAGAC CCTATCAGGA GAAGAGCTTG CGAAAGATGT TTGGAAACGG GCGTGCACGT
TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT GGAAAGTCCC TGGTTGGTGT GACTGCTGCA
TGCACTGTCA GAAAACGCTG TCTGGTGCTG GGCAACTCAG CTGTTTCTGT GGAGCAGTGG
AAAGCCCAGT TCAAGATGTG GTCCACCATT GACGACAGCC AGATCTGCCG GTTCACCTCC
GATGCCAAGG ACAAGCCCAT CGGCTGCTCC GTTGCCATTA GCACCTACTC CATGCTGGGC
CACACCACCA AAAGGTCCTG GGAGGCCGAG CGAGTCATGG AGTGGCTCAA GACCCAGGAG
TGGGGCCTCA TGATCCTGGA TGAAGTGCAC ACCATACCAG CCAAGATGTT CCGAAGGGTG
CTCACCATCG TGCAGGCCCA CTGTAAGCTG GGTTTGACTG CGACCCTCGT CCGCGAAGAT
GACAAAATTG TGGATTTAAA TTTTCTGATT GGGCCTAAGC TCTACGAAGC CAACTGGATG
GAGCTGCAGA ATAATGGCTA CATCGCCAAA GTCCAGTGTG CTGAGGTCTG GTGCCCTATG
TCTCCTGAAT TTTACCGGGA ATATGTGGCA ATCAAAACCA AGAAACGAAT CTTGCTGTAC
ACCATGAACC CCAACAAATT TAGAGCTTGC CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG
AATGACAAGA TTATTGTCTT TGCTGACAAT GTGTTTGCCC TAAAGGAATA TGCCATTCGA
CTGAACAAAC CCTATATCTA CGGACCTACG TCTCAGGGGG AAAGGATGCA AATTCTCCAG
AATTTCAAGC ACAACCCCAA AATTAACACC ATCTTCATAT CCAAGGTAGG TGACACTTCG
TTTGATCTGC CGGAAGCAAA TGTCCTCATT CAGATCTCAT CCCATGGTGG CTCCAGGCGT
CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT CGAGCTAAAA AAGGGATGGT TGCAGAAGAG
TACAATGCCT TTTTCTACTC ACTGGTATCC CAGGACACAC AGGAAATGGC TTACTCAACC
AAGCGGCAGA GATTCTTGGT AGATCAAGGT TATAGCTTCA AGGTGATCAC GAAACTCGCT
GGCATGGAGG AGGAAGACTT GGCGTTTTCG ACAAAAGAAG AGCAACAGCA GCTCTTACAG
AAAGTCCTGG CAGCCACTGA CCTGGATGCC GAGGAGGAGG TGGTGGCTGG GGAATTTGGC
TCCAGATCCA GCCAGGCATC TCGGCGCTTT GGCACCATGA GTTCTATGTC TGGGGCCGAC
GACACTGTGT ACATGGAGTA CCACTCATCG CGGAGCAAGG CGCCCAGCAA ACATGTACAC
CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011509096.1
CDS57..2423
Misc Feature(1)234..2411(+)
Misc Feature(2)279..656(+)
Misc Feature(3)1074..1484(+)
Misc Feature(4)1101..1115(+)
Misc Feature(5)1395..1406(+)
Misc Feature(6)1683..2012(+)
Misc Feature(7)1761..1904(+)
Misc Feature(8)1920..2009(+)
Translation

Target ORF information:

RefSeq Version XM_011510794
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011510794

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCCAC
CTACCTCTCT CACAGATTTC TAAGACTGCT GAAAGCAGTG GTGGGCCCTC CACTTCCCGA
GTGACAGATC CACAGGGTAA ATCTGACATC CCCATGGACC TGTTTGACTT CTATGAGCAA
ATGGACAAGG ATGAAGAAGA AGAAGAAGAG ACACAGACAG TGTCTTTTGA AGTCAAGCAG
GAAATGATTG AGGAACTCCA GAAACGTTGC ATCCACCTGG AGTACCCTCT GTTGGCAGAA
TATGACTTCC GGAATGATTC TGTCAACCCT GATATCAACA TTGACCTAAA GCCCACAGCT
GTCCTCAGAC CCTATCAGGA GAAGAGCTTG CGAAAGATGT TTGGAAACGG GCGTGCACGT
TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT GGAAAGTCCC TGGTTGGTGT GACTGCTGCA
TGCACTGTCA GAAAACGCTG TCTGGTGCTG GGCAACTCAG CTGTTTCTGT GGAGCAGTGG
AAAGCCCAGT TCAAGATGTG GTCCACCATT GACGACAGCC AGATCTGCCG GTTCACCTCC
GATGCCAAGG ACAAGCCCAT CGGCTGCTCC GTTGCCATTA GCACCTACTC CATGCTGGGC
CACACCACCA AAAGGTCCTG GGAGGCCGAG CGAGTCATGG AGTGGCTCAA GACCCAGGAG
TGGGGCCTCA TGATCCTGGA TGAAGTGCAC ACCATACCAG CCAAGATGTT CCGAAGGGTG
CTCACCATCG TGCAGGCCCA CTGTAAGCTG GGTTTGACTG CGACCCTCGT CCGCGAAGAT
GACAAAATTG TGGATTTAAA TTTTCTGATT GGGCCTAAGC TCTACGAAGC CAACTGGATG
GAGCTGCAGA ATAATGGCTA CATCGCCAAA GTCCAGTGTG CTGAGGTCTG GTGCCCTATG
TCTCCTGAAT TTTACCGGGA ATATGTGGCA ATCAAAACCA AGAAACGAAT CTTGCTGTAC
ACCATGAACC CCAACAAATT TAGAGCTTGC CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG
AATGACAAGA TTATTGTCTT TGCTGACAAT GTGTTTGCCC TAAAGGAATA TGCCATTCGA
CTGAACAAAC CCTATATCTA CGGACCTACG TCTCAGGGGG AAAGGATGCA AATTCTCCAG
AATTTCAAGC ACAACCCCAA AATTAACACC ATCTTCATAT CCAAGGTAGG TGACACTTCG
TTTGATCTGC CGGAAGCAAA TGTCCTCATT CAGATCTCAT CCCATGGTGG CTCCAGGCGT
CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT CGAGCTAAAA AAGGGATGGT TGCAGAAGAG
TACAATGCCT TTTTCTACTC ACTGGTATCC CAGGACACAC AGGAAATGGC TTACTCAACC
AAGCGGCAGA GATTCTTGGT AGATCAAGGT TATAGCTTCA AGGTGATCAC GAAACTCGCT
GGCATGGAGG AGGAAGACTT GGCGTTTTCG ACAAAAGAAG AGCAACAGCA GCTCTTACAG
AAAGTCCTGG CAGCCACTGA CCTGGATGCC GAGGAGGAGG TGGTGGCTGG GGAATTTGGC
TCCAGATCCA GCCAGGCATC TCGGCGCTTT GGCACCATGA GTTCTATGTC TGGGGCCGAC
GACACTGTGT ACATGGAGTA CCACTCATCG CGGAGCAAGG CGCCCAGCAA ACATGTACAC
CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57568
Clone ID Related Accession (Same CDS sequence) XM_011510795
Accession Version XM_011510795.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGTTTA TTAAGTTGTG TACTGTCAGC TATGGAAAAG TCAAGCTGGT CTTGAAGCAC 
AACAGATACT TCGTTGAAAG TTGCCACCCT GATGTAATCC AGCATCTTCT CCAGGACCCC
GTGATCCGAG AATGCCGCTT AAGAAACTCT GAAGGGGAGG CCACTGAGCT CATCACAGAG
ACTTTCACAA GCAAATCTGC CCACCTACCT CTCTCACAGA TTTCTAAGAC TGCTGAAAGC
AGTGGTGGGC CCTCCACTTC CCGAGTGACA GATCCACAGG GTAAATCTGA CATCCCCATG
GACCTGTTTG ACTTCTATGA GCAAATGGAC AAGGATGAAG AAGAAGAAGA AGAGACACAG
ACAGTGTCTT TTGAAGTCAA GCAGGAAATG ATTGAGGAAC TCCAGAAACG TTGCATCCAC
CTGGAGTACC CTCTGTTGGC AGAATATGAC TTCCGGAATG ATTCTGTCAA CCCTGATATC
AACATTGACC TAAAGCCCAC AGCTGTCCTC AGACCCTATC AGGAGAAGAG CTTGCGAAAG
ATGTTTGGAA ACGGGCGTGC ACGTTCGGGG GTCATTGTTC TTCCCTGCGG TGCTGGAAAG
TCCCTGGTTG GTGTGACTGC TGCATGCACT GTCAGAAAAC GCTGTCTGGT GCTGGGCAAC
TCAGCTGTTT CTGTGGAGCA GTGGAAAGCC CAGTTCAAGA TGTGGTCCAC CATTGACGAC
AGCCAGATCT GCCGGTTCAC CTCCGATGCC AAGGACAAGC CCATCGGCTG CTCCGTTGCC
ATTAGCACCT ACTCCATGCT GGGCCACACC ACCAAAAGGT CCTGGGAGGC CGAGCGAGTC
ATGGAGTGGC TCAAGACCCA GGAGTGGGGC CTCATGATCC TGGATGAAGT GCACACCATA
CCAGCCAAGA TGTTCCGAAG GGTGCTCACC ATCGTGCAGG CCCACTGTAA GCTGGGTTTG
ACTGCGACCC TCGTCCGCGA AGATGACAAA ATTGTGGATT TAAATTTTCT GATTGGGCCT
AAGCTCTACG AAGCCAACTG GATGGAGCTG CAGAATAATG GCTACATCGC CAAAGTCCAG
TGTGCTGAGG TCTGGTGCCC TATGTCTCCT GAATTTTACC GGGAATATGT GGCAATCAAA
ACCAAGAAAC GAATCTTGCT GTACACCATG AACCCCAACA AATTTAGAGC TTGCCAGTTT
CTGATCAAGT TTCATGAAAG GAGGAATGAC AAGATTATTG TCTTTGCTGA CAATGTGTTT
GCCCTAAAGG AATATGCCAT TCGACTGAAC AAACCCTATA TCTACGGACC TACGTCTCAG
GGGGAAAGGA TGCAAATTCT CCAGAATTTC AAGCACAACC CCAAAATTAA CACCATCTTC
ATATCCAAGG TAGGTGACAC TTCGTTTGAT CTGCCGGAAG CAAATGTCCT CATTCAGATC
TCATCCCATG GTGGCTCCAG GCGTCAGGAA GCCCAAAGGC TAGGGCGGGT GCTTCGAGCT
AAAAAAGGGA TGGTTGCAGA AGAGTACAAT GCCTTTTTCT ACTCACTGGT ATCCCAGGAC
ACACAGGAAA TGGCTTACTC AACCAAGCGG CAGAGATTCT TGGTAGATCA AGGTTATAGC
TTCAAGGTGA TCACGAAACT CGCTGGCATG GAGGAGGAAG ACTTGGCGTT TTCGACAAAA
GAAGAGCAAC AGCAGCTCTT ACAGAAAGTC CTGGCAGCCA CTGACCTGGA TGCCGAGGAG
GAGGTGGTGG CTGGGGAATT TGGCTCCAGA TCCAGCCAGG CATCTCGGCG CTTTGGCACC
ATGAGTTCTA TGTCTGGGGC CGACGACACT GTGTACATGG AGTACCACTC ATCGCGGAGC
AAGGCGCCCA GCAAACATGT ACACCCGCTC TTCAAGCGCT TTAGGAAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011509097.1
CDS387..2297
Misc Feature(1)828..2048(+)
Misc Feature(2)948..1358(+)
Misc Feature(3)975..989(+)
Misc Feature(4)1269..1280(+)
Misc Feature(5)1557..1886(+)
Misc Feature(6)1635..1778(+)
Misc Feature(7)1794..1883(+)
Translation

Target ORF information:

RefSeq Version XM_011510795
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011510795

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGTTTA TTAAGTTGTG TACTGTCAGC TATGGAAAAG TCAAGCTGGT CTTGAAGCAC 
AACAGATACT TCGTTGAAAG TTGCCACCCT GATGTAATCC AGCATCTTCT CCAGGACCCC
GTGATCCGAG AATGCCGCTT AAGAAACTCT GAAGGGGAGG CCACTGAGCT CATCACAGAG
ACTTTCACAA GCAAATCTGC CCACCTACCT CTCTCACAGA TTTCTAAGAC TGCTGAAAGC
AGTGGTGGGC CCTCCACTTC CCGAGTGACA GATCCACAGG GTAAATCTGA CATCCCCATG
GACCTGTTTG ACTTCTATGA GCAAATGGAC AAGGATGAAG AAGAAGAAGA AGAGACACAG
ACAGTGTCTT TTGAAGTCAA GCAGGAAATG ATTGAGGAAC TCCAGAAACG TTGCATCCAC
CTGGAGTACC CTCTGTTGGC AGAATATGAC TTCCGGAATG ATTCTGTCAA CCCTGATATC
AACATTGACC TAAAGCCCAC AGCTGTCCTC AGACCCTATC AGGAGAAGAG CTTGCGAAAG
ATGTTTGGAA ACGGGCGTGC ACGTTCGGGG GTCATTGTTC TTCCCTGCGG TGCTGGAAAG
TCCCTGGTTG GTGTGACTGC TGCATGCACT GTCAGAAAAC GCTGTCTGGT GCTGGGCAAC
TCAGCTGTTT CTGTGGAGCA GTGGAAAGCC CAGTTCAAGA TGTGGTCCAC CATTGACGAC
AGCCAGATCT GCCGGTTCAC CTCCGATGCC AAGGACAAGC CCATCGGCTG CTCCGTTGCC
ATTAGCACCT ACTCCATGCT GGGCCACACC ACCAAAAGGT CCTGGGAGGC CGAGCGAGTC
ATGGAGTGGC TCAAGACCCA GGAGTGGGGC CTCATGATCC TGGATGAAGT GCACACCATA
CCAGCCAAGA TGTTCCGAAG GGTGCTCACC ATCGTGCAGG CCCACTGTAA GCTGGGTTTG
ACTGCGACCC TCGTCCGCGA AGATGACAAA ATTGTGGATT TAAATTTTCT GATTGGGCCT
AAGCTCTACG AAGCCAACTG GATGGAGCTG CAGAATAATG GCTACATCGC CAAAGTCCAG
TGTGCTGAGG TCTGGTGCCC TATGTCTCCT GAATTTTACC GGGAATATGT GGCAATCAAA
ACCAAGAAAC GAATCTTGCT GTACACCATG AACCCCAACA AATTTAGAGC TTGCCAGTTT
CTGATCAAGT TTCATGAAAG GAGGAATGAC AAGATTATTG TCTTTGCTGA CAATGTGTTT
GCCCTAAAGG AATATGCCAT TCGACTGAAC AAACCCTATA TCTACGGACC TACGTCTCAG
GGGGAAAGGA TGCAAATTCT CCAGAATTTC AAGCACAACC CCAAAATTAA CACCATCTTC
ATATCCAAGG TAGGTGACAC TTCGTTTGAT CTGCCGGAAG CAAATGTCCT CATTCAGATC
TCATCCCATG GTGGCTCCAG GCGTCAGGAA GCCCAAAGGC TAGGGCGGGT GCTTCGAGCT
AAAAAAGGGA TGGTTGCAGA AGAGTACAAT GCCTTTTTCT ACTCACTGGT ATCCCAGGAC
ACACAGGAAA TGGCTTACTC AACCAAGCGG CAGAGATTCT TGGTAGATCA AGGTTATAGC
TTCAAGGTGA TCACGAAACT CGCTGGCATG GAGGAGGAAG ACTTGGCGTT TTCGACAAAA
GAAGAGCAAC AGCAGCTCTT ACAGAAAGTC CTGGCAGCCA CTGACCTGGA TGCCGAGGAG
GAGGTGGTGG CTGGGGAATT TGGCTCCAGA TCCAGCCAGG CATCTCGGCG CTTTGGCACC
ATGAGTTCTA TGTCTGGGGC CGACGACACT GTGTACATGG AGTACCACTC ATCGCGGAGC
AAGGCGCCCA GCAAACATGT ACACCCGCTC TTCAAGCGCT TTAGGAAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35980
Clone ID Related Accession (Same CDS sequence) NM_001303416 , NM_001303418
Accession Version NM_001303416.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-JUN-2015
Organism Homo sapiens (human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095557.1, AK091500.1 and AC110926.5. On Dec 31, 2014 this sequence version replaced gi:530369092. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 3 encodes the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK091500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290345.1
CDS403..2559
Misc Feature(1)43..45(+)
Misc Feature(2)403..2547(+)
Misc Feature(3)433..810(+)
Misc Feature(4)1210..1620(+)
Misc Feature(5)1237..1251(+)
Misc Feature(6)1531..1542(+)
Misc Feature(7)1819..2148(+)
Misc Feature(8)1897..2040(+)
Misc Feature(9)2056..2145(+)
Exon (1)1..238
Gene:ERCC3
Gene Synonym:
Exon (2)239..444
Gene:ERCC3
Gene Synonym:
Exon (3)445..681
Gene:ERCC3
Gene Synonym:
Exon (4)682..731
Gene:ERCC3
Gene Synonym:
Exon (5)732..867
Gene:ERCC3
Gene Synonym:
Exon (6)868..1032
Gene:ERCC3
Gene Synonym:
Exon (7)1033..1237
Gene:ERCC3
Gene Synonym:
Exon (8)1238..1552
Gene:ERCC3
Gene Synonym:
Exon (9)1553..1737
Gene:ERCC3
Gene Synonym:
Exon (10)1738..1940
Gene:ERCC3
Gene Synonym:
Exon (11)1941..2037
Gene:ERCC3
Gene Synonym:
Exon (12)2038..2155
Gene:ERCC3
Gene Synonym:
Exon (13)2156..2274
Gene:ERCC3
Gene Synonym:
Exon (14)2275..2427
Gene:ERCC3
Gene Synonym:
Exon (15)2428..2865
Gene:ERCC3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001303416
Organism Homo sapiens (human)
Definition Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303416

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35980
Clone ID Related Accession (Same CDS sequence) NM_001303416 , NM_001303418
Accession Version NM_001303418.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-JUN-2015
Organism Homo sapiens (human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095557.1, AC110926.5 and AI222871.1. Transcript Variant: This variant (3) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 3 encodes the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK095557.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290347.1
CDS465..2621
Misc Feature(1)276..278(+)
Misc Feature(2)465..2609(+)
Misc Feature(3)495..872(+)
Misc Feature(4)1272..1682(+)
Misc Feature(5)1299..1313(+)
Misc Feature(6)1593..1604(+)
Misc Feature(7)1881..2210(+)
Misc Feature(8)1959..2102(+)
Misc Feature(9)2118..2207(+)
Exon (1)1..300
Gene:ERCC3
Gene Synonym:
Exon (2)301..506
Gene:ERCC3
Gene Synonym:
Exon (3)507..743
Gene:ERCC3
Gene Synonym:
Exon (4)744..793
Gene:ERCC3
Gene Synonym:
Exon (5)794..929
Gene:ERCC3
Gene Synonym:
Exon (6)930..1094
Gene:ERCC3
Gene Synonym:
Exon (7)1095..1299
Gene:ERCC3
Gene Synonym:
Exon (8)1300..1614
Gene:ERCC3
Gene Synonym:
Exon (9)1615..1799
Gene:ERCC3
Gene Synonym:
Exon (10)1800..2002
Gene:ERCC3
Gene Synonym:
Exon (11)2003..2099
Gene:ERCC3
Gene Synonym:
Exon (12)2100..2217
Gene:ERCC3
Gene Synonym:
Exon (13)2218..2336
Gene:ERCC3
Gene Synonym:
Exon (14)2337..2489
Gene:ERCC3
Gene Synonym:
Exon (15)2490..2927
Gene:ERCC3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001303418
Organism Homo sapiens (human)
Definition Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303418

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25443
Clone ID Related Accession (Same CDS sequence) NM_000122
Accession Version NM_000122.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-JUN-2015
Organism Homo sapiens (human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M31899.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M31899.1, AK222465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCATT
TCTAAGACTG CTGAAAGCAG TGGTGGGCCC TCCACTTCCC GAGTGACAGA TCCACAGGGT
AAATCTGACA TCCCCATGGA CCTGTTTGAC TTCTATGAGC AAATGGACAA GGATGAAGAA
GAAGAAGAAG AGACACAGAC AGTGTCTTTT GAAGTCAAGC AGGAAATGAT TGAGGAACTC
CAGAAACGTT GCATCCACCT GGAGTACCCT CTGTTGGCAG AATATGACTT CCGGAATGAT
TCTGTCAACC CTGATATCAA CATTGACCTA AAGCCCACAG CTGTCCTCAG ACCCTATCAG
GAGAAGAGCT TGCGAAAGAT GTTTGGAAAC GGGCGTGCAC GTTCGGGGGT CATTGTTCTT
CCCTGCGGTG CTGGAAAGTC CCTGGTTGGT GTGACTGCTG CATGCACTGT CAGAAAACGC
TGTCTGGTGC TGGGCAACTC AGCTGTTTCT GTGGAGCAGT GGAAAGCCCA GTTCAAGATG
TGGTCCACCA TTGACGACAG CCAGATCTGC CGGTTCACCT CCGATGCCAA GGACAAGCCC
ATCGGCTGCT CCGTTGCCAT TAGCACCTAC TCCATGCTGG GCCACACCAC CAAAAGGTCC
TGGGAGGCCG AGCGAGTCAT GGAGTGGCTC AAGACCCAGG AGTGGGGCCT CATGATCCTG
GATGAAGTGC ACACCATACC AGCCAAGATG TTCCGAAGGG TGCTCACCAT CGTGCAGGCC
CACTGTAAGC TGGGTTTGAC TGCGACCCTC GTCCGCGAAG ATGACAAAAT TGTGGATTTA
AATTTTCTGA TTGGGCCTAA GCTCTACGAA GCCAACTGGA TGGAGCTGCA GAATAATGGC
TACATCGCCA AAGTCCAGTG TGCTGAGGTC TGGTGCCCTA TGTCTCCTGA ATTTTACCGG
GAATATGTGG CAATCAAAAC CAAGAAACGA ATCTTGCTGT ACACCATGAA CCCCAACAAA
TTTAGAGCTT GCCAGTTTCT GATCAAGTTT CATGAAAGGA GGAATGACAA GATTATTGTC
TTTGCTGACA ATGTGTTTGC CCTAAAGGAA TATGCCATTC GACTGAACAA ACCCTATATC
TACGGACCTA CGTCTCAGGG GGAAAGGATG CAAATTCTCC AGAATTTCAA GCACAACCCC
AAAATTAACA CCATCTTCAT ATCCAAGGTA GGTGACACTT CGTTTGATCT GCCGGAAGCA
AATGTCCTCA TTCAGATCTC ATCCCATGGT GGCTCCAGGC GTCAGGAAGC CCAAAGGCTA
GGGCGGGTGC TTCGAGCTAA AAAAGGGATG GTTGCAGAAG AGTACAATGC CTTTTTCTAC
TCACTGGTAT CCCAGGACAC ACAGGAAATG GCTTACTCAA CCAAGCGGCA GAGATTCTTG
GTAGATCAAG GTTATAGCTT CAAGGTGATC ACGAAACTCG CTGGCATGGA GGAGGAAGAC
TTGGCGTTTT CGACAAAAGA AGAGCAACAG CAGCTCTTAC AGAAAGTCCT GGCAGCCACT
GACCTGGATG CCGAGGAGGA GGTGGTGGCT GGGGAATTTG GCTCCAGATC CAGCCAGGCA
TCTCGGCGCT TTGGCACCAT GAGTTCTATG TCTGGGGCCG ACGACACTGT GTACATGGAG
TACCACTCAT CGCGGAGCAA GGCGCCCAGC AAACATGTAC ACCCGCTCTT CAAGCGCTTT
AGGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000113.1
CDS96..2444
Misc Feature(1)15..17(+)
Misc Feature(2)41..41(+)
Misc Feature(3)111..149(+)
Misc Feature(4)273..2432(+)
Misc Feature(5)318..695(+)
Misc Feature(6)1095..1505(+)
Misc Feature(7)1122..1136(+)
Misc Feature(8)1203..1205(+)
Misc Feature(9)1416..1427(+)
Misc Feature(10)1416..1427(+)
Misc Feature(11)1704..2033(+)
Misc Feature(12)1782..1925(+)
Misc Feature(13)1941..2030(+)
Misc Feature(14)2346..2348(+)
Exon (1)1..123
Gene:ERCC3
Gene Synonym:
Exon (2)124..329
Gene:ERCC3
Gene Synonym:
Exon (3)330..566
Gene:ERCC3
Gene Synonym:
Exon (4)567..616
Gene:ERCC3
Gene Synonym:
Exon (5)617..752
Gene:ERCC3
Gene Synonym:
Exon (6)753..917
Gene:ERCC3
Gene Synonym:
Exon (7)918..1122
Gene:ERCC3
Gene Synonym:
Exon (8)1123..1437
Gene:ERCC3
Gene Synonym:
Exon (9)1438..1622
Gene:ERCC3
Gene Synonym:
Exon (10)1623..1825
Gene:ERCC3
Gene Synonym:
Exon (11)1826..1922
Gene:ERCC3
Gene Synonym:
Exon (12)1923..2040
Gene:ERCC3
Gene Synonym:
Exon (13)2041..2159
Gene:ERCC3
Gene Synonym:
Exon (14)2160..2312
Gene:ERCC3
Gene Synonym:
Exon (15)2313..2750
Gene:ERCC3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000122
Organism Homo sapiens (human)
Definition Homo sapiens excision repair cross-complementation group 3 (ERCC3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000122

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCATT
TCTAAGACTG CTGAAAGCAG TGGTGGGCCC TCCACTTCCC GAGTGACAGA TCCACAGGGT
AAATCTGACA TCCCCATGGA CCTGTTTGAC TTCTATGAGC AAATGGACAA GGATGAAGAA
GAAGAAGAAG AGACACAGAC AGTGTCTTTT GAAGTCAAGC AGGAAATGAT TGAGGAACTC
CAGAAACGTT GCATCCACCT GGAGTACCCT CTGTTGGCAG AATATGACTT CCGGAATGAT
TCTGTCAACC CTGATATCAA CATTGACCTA AAGCCCACAG CTGTCCTCAG ACCCTATCAG
GAGAAGAGCT TGCGAAAGAT GTTTGGAAAC GGGCGTGCAC GTTCGGGGGT CATTGTTCTT
CCCTGCGGTG CTGGAAAGTC CCTGGTTGGT GTGACTGCTG CATGCACTGT CAGAAAACGC
TGTCTGGTGC TGGGCAACTC AGCTGTTTCT GTGGAGCAGT GGAAAGCCCA GTTCAAGATG
TGGTCCACCA TTGACGACAG CCAGATCTGC CGGTTCACCT CCGATGCCAA GGACAAGCCC
ATCGGCTGCT CCGTTGCCAT TAGCACCTAC TCCATGCTGG GCCACACCAC CAAAAGGTCC
TGGGAGGCCG AGCGAGTCAT GGAGTGGCTC AAGACCCAGG AGTGGGGCCT CATGATCCTG
GATGAAGTGC ACACCATACC AGCCAAGATG TTCCGAAGGG TGCTCACCAT CGTGCAGGCC
CACTGTAAGC TGGGTTTGAC TGCGACCCTC GTCCGCGAAG ATGACAAAAT TGTGGATTTA
AATTTTCTGA TTGGGCCTAA GCTCTACGAA GCCAACTGGA TGGAGCTGCA GAATAATGGC
TACATCGCCA AAGTCCAGTG TGCTGAGGTC TGGTGCCCTA TGTCTCCTGA ATTTTACCGG
GAATATGTGG CAATCAAAAC CAAGAAACGA ATCTTGCTGT ACACCATGAA CCCCAACAAA
TTTAGAGCTT GCCAGTTTCT GATCAAGTTT CATGAAAGGA GGAATGACAA GATTATTGTC
TTTGCTGACA ATGTGTTTGC CCTAAAGGAA TATGCCATTC GACTGAACAA ACCCTATATC
TACGGACCTA CGTCTCAGGG GGAAAGGATG CAAATTCTCC AGAATTTCAA GCACAACCCC
AAAATTAACA CCATCTTCAT ATCCAAGGTA GGTGACACTT CGTTTGATCT GCCGGAAGCA
AATGTCCTCA TTCAGATCTC ATCCCATGGT GGCTCCAGGC GTCAGGAAGC CCAAAGGCTA
GGGCGGGTGC TTCGAGCTAA AAAAGGGATG GTTGCAGAAG AGTACAATGC CTTTTTCTAC
TCACTGGTAT CCCAGGACAC ACAGGAAATG GCTTACTCAA CCAAGCGGCA GAGATTCTTG
GTAGATCAAG GTTATAGCTT CAAGGTGATC ACGAAACTCG CTGGCATGGA GGAGGAAGAC
TTGGCGTTTT CGACAAAAGA AGAGCAACAG CAGCTCTTAC AGAAAGTCCT GGCAGCCACT
GACCTGGATG CCGAGGAGGA GGTGGTGGCT GGGGAATTTG GCTCCAGATC CAGCCAGGCA
TCTCGGCGCT TTGGCACCAT GAGTTCTATG TCTGGGGCCG ACGACACTGT GTACATGGAG
TACCACTCAT CGCGGAGCAA GGCGCCCAGC AAACATGTAC ACCCGCTCTT CAAGCGCTTT
AGGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*