×

ALAS1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ALAS1
Entrez Gene ID 211
Full Name 5'-aminolevulinate synthase 1
Synonyms ALAS, ALAS-H, ALAS3, ALASH, MIG4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.1

Summary This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]. lac of sum
Disorder MIM:

125290


mRNA and Protein(s)

mRNA Protein Name
XM_011533477 XP_011531779 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
XM_011533478 XP_011531780 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X2
NM_000688 NP_000679 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
NM_001304444 NP_001291373 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 2
NM_199166 NP_954635 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
NM_001304443 NP_001291372 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor



Homo sapiens (human) ALAS1 NP_000679.1
Pan troglodytes (chimpanzee) ALAS1 XP_001171508.2
Macaca mulatta (Rhesus monkey) ALAS1 XP_001090675.1
Canis lupus familiaris (dog) ALAS1 XP_533804.3
Bos taurus (cattle) ALAS1 NP_001094624.1
Mus musculus (house mouse) Alas1 NP_065584.2
Rattus norvegicus (Norway rat) Alas1 NP_077810.2
Gallus gallus (chicken) ALAS1 NP_001018012.1
Danio rerio (zebrafish) alas1 NP_958444.1
Xenopus (Silurana) tropicalis (western clawed frog) alas1 NP_001008072.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56227 XM_011533477 PREDICTED: Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu56228 XM_011533478 PREDICTED: Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu14916
NM_000688 Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu37039 NM_001304444 Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu14916
NM_199166 Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu14916
NM_001304443 Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu56227
Clone ID Related Accession (Same CDS sequence) XM_011533477
Accession Version XM_011533477.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1836bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
AACTACTTCC TTGTTTCTCC TCAGAGAATC TGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531779.1
CDS280..2115
Misc Feature(1)325..747(+)
Misc Feature(2)919..2091(+)
Misc Feature(3)1057..2091(+)
Misc Feature(4)1081..2043(+)
Misc Feature(5)1261..1665(+)
Misc Feature(6)1663..1665(+)
Translation

Target ORF information:

RefSeq Version XM_011533477
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533477

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
AACTACTTCC TTGTTTCTCC TCAGAGAATC TGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56228
Clone ID Related Accession (Same CDS sequence) XM_011533478
Accession Version XM_011533478.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
GTCCATTCAA TGGATGGCAA AGCCTTTGGT TGTGTTGGAG GGTACATCGC CAGCACGAGT
TCTCTGATTG ACACCGTACG GTCCTATGCT GCTGGCTTCA TCTTCACCAC CTCTCTGCCA
CCCATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCGC TGAGGGACGG
GTGCTTCGCC GCCAGCACCA GCGCAACGTC AAACTCATGA GACAGATGCT AATGGATGCC
GGCCTCCCTG TTGTCCACTG CCCCAGCCAC ATCATCCCTG TGCGGGTTGC AGATGCTGCT
AAAAACACAG AAGTCTGTGA TGAACTAATG AGCAGACATA ACATCTACGT GCAAGCAATC
AATTACCCTA CGGTGCCCCG GGGAGAAGAG CTCCTACGGA TTGCCCCCAC CCCTCACCAC
ACACCCCAGA TGATGAACTA CTTCCTTGAG AATCTGCTAG TCACATGGAA GCAAGTGGGG
CTGGAACTGA AGCCTCATTC CTCAGCTGAG TGCAACTTCT GCAGGAGGCC ACTGCATTTT
GAAGTGATGA GTGAAAGAGA GAAGTCCTAT TTCTCAGGCT TGAGCAAGTT GGTATCTGCT
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531780.1
CDS281..2089
Misc Feature(1)326..748(+)
Misc Feature(2)920..1930(+)
Misc Feature(3)1058..1960(+)
Misc Feature(4)1082..1879(+)
Misc Feature(5)1262..1501(+)
Misc Feature(6)1499..1501(+)
Translation

Target ORF information:

RefSeq Version XM_011533478
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533478

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
GTCCATTCAA TGGATGGCAA AGCCTTTGGT TGTGTTGGAG GGTACATCGC CAGCACGAGT
TCTCTGATTG ACACCGTACG GTCCTATGCT GCTGGCTTCA TCTTCACCAC CTCTCTGCCA
CCCATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCGC TGAGGGACGG
GTGCTTCGCC GCCAGCACCA GCGCAACGTC AAACTCATGA GACAGATGCT AATGGATGCC
GGCCTCCCTG TTGTCCACTG CCCCAGCCAC ATCATCCCTG TGCGGGTTGC AGATGCTGCT
AAAAACACAG AAGTCTGTGA TGAACTAATG AGCAGACATA ACATCTACGT GCAAGCAATC
AATTACCCTA CGGTGCCCCG GGGAGAAGAG CTCCTACGGA TTGCCCCCAC CCCTCACCAC
ACACCCCAGA TGATGAACTA CTTCCTTGAG AATCTGCTAG TCACATGGAA GCAAGTGGGG
CTGGAACTGA AGCCTCATTC CTCAGCTGAG TGCAACTTCT GCAGGAGGCC ACTGCATTTT
GAAGTGATGA GTGAAAGAGA GAAGTCCTAT TTCTCAGGCT TGAGCAAGTT GGTATCTGCT
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14916
Clone ID Related Accession (Same CDS sequence) NM_000688 , NM_001304443 , NM_199166
Accession Version NM_000688.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC097637.2, AU279391.1, BC011798.2, CD014147.1, X56351.1 and AI868691.1. On Dec 15, 2011 this sequence version replaced gi:40316942. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AY260745.1, BC011798.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000679.1
CDS383..2305
Misc Feature(1)326..328(+)
Misc Feature(2)383..799(+)
Misc Feature(3)971..2146(+)
Misc Feature(4)1109..2176(+)
Misc Feature(5)1133..2095(+)
Misc Feature(6)1313..1717(+)
Misc Feature(7)1715..1717(+)
Misc Feature(8)1715..1717(+)
Exon (1)1..173
Gene:ALAS1
Gene Synonym:
Exon (2)174..350
Gene:ALAS1
Gene Synonym:
Exon (3)351..581
Gene:ALAS1
Gene Synonym:
Exon (4)582..809
Gene:ALAS1
Gene Synonym:
Exon (5)810..959
Gene:ALAS1
Gene Synonym:
Exon (6)960..1182
Gene:ALAS1
Gene Synonym:
Exon (7)1183..1367
Gene:ALAS1
Gene Synonym:
Exon (8)1368..1547
Gene:ALAS1
Gene Synonym:
Exon (9)1548..1712
Gene:ALAS1
Gene Synonym:
Exon (10)1713..1981
Gene:ALAS1
Gene Synonym:
Exon (11)1982..2144
Gene:ALAS1
Gene Synonym:
Exon (12)2145..2452
Gene:ALAS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000688
Organism Homo sapiens (human)
Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000688

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37039
Clone ID Related Accession (Same CDS sequence) NM_001304444
Accession Version NM_001304444.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM546974.1, DA691060.1, AK300993.1 and AB063322.1. On Jan 27, 2015 this sequence version replaced gi:578805771. Transcript Variant: This variant (4) uses an alternate splice site in its 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK300993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
AACTACTTCC TTGAGAATCT GCTAGTCACA TGGAAGCAAG TGGGGCTGGA ACTGAAGCCT
CATTCCTCAG CTGAGTGCAA CTTCTGCAGG AGGCCACTGC ATTTTGAAGT GATGAGTGAA
AGAGAGAAGT CCTATTTCTC AGGCTTGAGC AAGTTGGTAT CTGCTCAGGC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291373.1
CDS321..2294
Misc Feature(1)54..56(+)
Misc Feature(2)366..788(+)
Misc Feature(3)960..2135(+)
Misc Feature(4)1098..2165(+)
Misc Feature(5)1122..2084(+)
Misc Feature(6)1302..1706(+)
Misc Feature(7)1704..1706(+)
Exon (1)1..173
Gene:ALAS1
Gene Synonym:
Exon (2)174..350
Gene:ALAS1
Gene Synonym:
Exon (3)351..570
Gene:ALAS1
Gene Synonym:
Exon (4)571..798
Gene:ALAS1
Gene Synonym:
Exon (5)799..948
Gene:ALAS1
Gene Synonym:
Exon (6)949..1171
Gene:ALAS1
Gene Synonym:
Exon (7)1172..1356
Gene:ALAS1
Gene Synonym:
Exon (8)1357..1536
Gene:ALAS1
Gene Synonym:
Exon (9)1537..1701
Gene:ALAS1
Gene Synonym:
Exon (10)1702..1970
Gene:ALAS1
Gene Synonym:
Exon (11)1971..2133
Gene:ALAS1
Gene Synonym:
Exon (12)2134..2441
Gene:ALAS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304444
Organism Homo sapiens (human)
Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304444

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
AACTACTTCC TTGAGAATCT GCTAGTCACA TGGAAGCAAG TGGGGCTGGA ACTGAAGCCT
CATTCCTCAG CTGAGTGCAA CTTCTGCAGG AGGCCACTGC ATTTTGAAGT GATGAGTGAA
AGAGAGAAGT CCTATTTCTC AGGCTTGAGC AAGTTGGTAT CTGCTCAGGC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14916
Clone ID Related Accession (Same CDS sequence) NM_000688 , NM_001304443 , NM_199166
Accession Version NM_199166.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM546974.1, BU173391.1, X56351.1 and AI868691.1. On Dec 15, 2011 this sequence version replaced gi:40316938. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB063322.1, AK312566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_954635.1
CDS206..2128
Misc Feature(1)128..130(+)
Misc Feature(2)206..622(+)
Misc Feature(3)794..1969(+)
Misc Feature(4)932..1999(+)
Misc Feature(5)956..1918(+)
Misc Feature(6)1136..1540(+)
Misc Feature(7)1538..1540(+)
Misc Feature(8)1538..1540(+)
Exon (1)1..173
Gene:ALAS1
Gene Synonym:
Exon (2)174..404
Gene:ALAS1
Gene Synonym:
Exon (3)405..632
Gene:ALAS1
Gene Synonym:
Exon (4)633..782
Gene:ALAS1
Gene Synonym:
Exon (5)783..1005
Gene:ALAS1
Gene Synonym:
Exon (6)1006..1190
Gene:ALAS1
Gene Synonym:
Exon (7)1191..1370
Gene:ALAS1
Gene Synonym:
Exon (8)1371..1535
Gene:ALAS1
Gene Synonym:
Exon (9)1536..1804
Gene:ALAS1
Gene Synonym:
Exon (10)1805..1967
Gene:ALAS1
Gene Synonym:
Exon (11)1968..2275
Gene:ALAS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_199166
Organism Homo sapiens (human)
Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_199166

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14916
Clone ID Related Accession (Same CDS sequence) NM_000688 , NM_001304443 , NM_199166
Accession Version NM_001304443.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM546974.1, DA691060.1, Y00451.1, AK300993.1 and AB063322.1. On Jan 27, 2015 this sequence version replaced gi:530372052. Transcript Variant: This variant (3) lacks an alternate exon and uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291372.1
CDS195..2117
Misc Feature(1)54..56(+)
Misc Feature(2)195..611(+)
Misc Feature(3)783..1958(+)
Misc Feature(4)921..1988(+)
Misc Feature(5)945..1907(+)
Misc Feature(6)1125..1529(+)
Misc Feature(7)1527..1529(+)
Misc Feature(8)1527..1529(+)
Exon (1)1..173
Gene:ALAS1
Gene Synonym:
Exon (2)174..393
Gene:ALAS1
Gene Synonym:
Exon (3)394..621
Gene:ALAS1
Gene Synonym:
Exon (4)622..771
Gene:ALAS1
Gene Synonym:
Exon (5)772..994
Gene:ALAS1
Gene Synonym:
Exon (6)995..1179
Gene:ALAS1
Gene Synonym:
Exon (7)1180..1359
Gene:ALAS1
Gene Synonym:
Exon (8)1360..1524
Gene:ALAS1
Gene Synonym:
Exon (9)1525..1793
Gene:ALAS1
Gene Synonym:
Exon (10)1794..1956
Gene:ALAS1
Gene Synonym:
Exon (11)1957..2264
Gene:ALAS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304443
Organism Homo sapiens (human)
Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304443

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*