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EWSR1 EWS RNA-binding protein 1 [Homo sapiens (human)]

Gene Symbol EWSR1
Entrez Gene ID 2130
Full Name EWS RNA-binding protein 1
Synonyms EWS, EWS-FLI1, bK984G1.4
General protein information
Preferred Names
RNA-binding protein EWS
Names
RNA-binding protein EWS
Ewing sarcoma breakpoint region 1
Ewings sarcoma EWS-Fli1 (type 1) oncogene
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q12.2

Summary This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]. lac of sum
Disorder MIM:

133450

Disorder Html: Ewing sarcoma, 612219 (3); Neuroepithelioma, 612219 (3)





The following EWSR1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EWSR1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu57612 XM_011529995 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu53446 XM_005261389 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu57613 XM_011529996 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu57614 XM_011529997 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu57615 XM_011529998 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu57616 XM_011529999 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu57617 XM_011530000 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu57618 XM_011530001 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu53449 XM_005261390 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu57619 XM_011530002 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu27045 NM_005243 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $439
OHu26751 NM_001163287 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector in pcDNA3.1+/C-(K)DYK $379
OHu26616 NM_001163286 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $499
OHu27250 NM_001163285 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 Quote Price
OHu27117 NM_013986 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu57612
Accession Version XM_011529995.1
Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TAAGGCCCCA
AAGCCTGAAG GCTTCCTCCC GCCACCCTTT CCGCCCCCGG GTGGTGATCG TGGCAGAGGT
GGCCCTGGTG GCATGCGGGG AGGAAGAGGT GGCCTCATGG ATCGTGGTGG TCCCGGTGGA
ATGTTCAGAG GTGGCCGTGG TGGAGACAGA GGTGGCTTCC GTGGTGGCCG GGGCATGGAC
CGAGGTGGCT TTGGTGGAGG AAGACGAGGT GGCCCTGGGG GGCCCCCTGG ACCTTTGATG
GAACAGATGG GAGGAAGAAG AGGAGGACGT GGAGGACCTG GAAAAATGGA TAAAGGCGAG
CACCGTCAGG AGCGCAGAGA TCGGCCCTAC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528297.1
CDS56..2068
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..1738(+)
Misc Feature(4)1622..1726(+)
Misc Feature(5)1628..1726(+)
Translation
Position Chain Variation Link
3 3 a, c dbSNP:551097984
5 5 c, g dbSNP:770561780
7 7 a, c, g dbSNP:758447329
8 8 a, c dbSNP:758975710
11 11 a, c, t dbSNP:111892587
14 14 a, g dbSNP:367933864
19 19 a, c dbSNP:763833724
21 21 a, g dbSNP:751395202
22 22 a, c, g dbSNP:756462778
23 23 a, g dbSNP:754003425
25 25 a, g dbSNP:754966628
27 27 -, g dbSNP:760330065
31 31 a, g dbSNP:199589576
35 35 a, t dbSNP:748647019
36 36 a, g dbSNP:758943413
37 37 c, g dbSNP:201886504
38 38 c, g, t dbSNP:377068270
39 39 a, g dbSNP:776222972
41 41 c, g dbSNP:745403052
47 47 a, g dbSNP:375095904
48 48 a, g dbSNP:552992152
52 52 c, g dbSNP:762864394
53 53 a, g dbSNP:2231392
62 62 a, t dbSNP:774293267
64 64 c, t dbSNP:779516758
65 65 a, c dbSNP:761661070
67 67 a, c, g dbSNP:147203739
77 77 a, g dbSNP:772125233
79 79 c, t dbSNP:201792944
81 81 a, g dbSNP:768830118
82 82 c, t dbSNP:140505486
89 89 a, g dbSNP:769936524
97 97 a, g dbSNP:775729620
103 103 a, g dbSNP:763137483
121 121 c, t dbSNP:377732811
122 122 a, g dbSNP:777722463
123 123 c, t dbSNP:746797640
124 124 c, t dbSNP:770806014
131 131 a, g, t dbSNP:776256103
161 161 a, g dbSNP:753624585
163 163 a, c, g dbSNP:141206174
177 177 a, g dbSNP:765008447
180 180 a, g dbSNP:752450196
187 187 c, g dbSNP:762485960
189 189 a, g dbSNP:374454766
190 190 c, t dbSNP:763729441
193 193 a, g dbSNP:751022322
216 216 c, g dbSNP:757233633
224 224 c, g dbSNP:781041301
226 226 c, g dbSNP:150734586
230 230 a, c dbSNP:755951683
238 238 c, t dbSNP:779793507
247 247 c, g, t dbSNP:748434313
248 248 a, t dbSNP:777919893
250 250 c, t dbSNP:573011438
255 255 a, g dbSNP:771496966
259 259 a, g dbSNP:139048680
260 260 a, g dbSNP:368705995
268 268 c, t dbSNP:770326364
271 271 a, g dbSNP:775996281
278 278 c, t dbSNP:762726444
281 281 a, g dbSNP:556275941
296 296 a, c dbSNP:778130614
299 299 a, g dbSNP:768951742
302 302 a, g dbSNP:182195687
304 304 a, c dbSNP:747812796
318 318 a, g dbSNP:377199824
325 325 c, t dbSNP:772711384
327 327 c, t dbSNP:760136386
330 330 a, c dbSNP:766322894
331 331 a, g dbSNP:776479146
332 332 c, g dbSNP:759489827
334 334 g, t dbSNP:765011656
351 351 -, atg dbSNP:769502605
357 357 a, c dbSNP:752526721
359 359 a, g dbSNP:757568499
362 362 a, g dbSNP:370033991
370 370 a, g, t dbSNP:750563135
373 373 -, cac dbSNP:775127785
373 373 a, c dbSNP:780159761
376 376 c, t dbSNP:749826758
383 383 c, g dbSNP:755496976
388 388 c, t dbSNP:779156750
389 389 g, t dbSNP:748442517
402 402 a, g dbSNP:771741224
409 409 a, g dbSNP:373180201
415 415 c, t dbSNP:746637617
417 417 c, g dbSNP:770384643
427 427 c, t dbSNP:776204033
430 430 c, t dbSNP:759453504
438 438 a, g dbSNP:559661693
442 442 c, g dbSNP:775155431
446 446 c, g dbSNP:762797076
450 450 a, c dbSNP:200282253
458 458 a, g dbSNP:750724221
462 462 c, t dbSNP:760949110
467 467 a, g dbSNP:149885670
470 470 a, c dbSNP:753897217
474 474 c, g, t dbSNP:765207539
475 475 a, c, g dbSNP:758898123
487 487 c, g dbSNP:200637771
493 493 c, t dbSNP:146418156
507 507 a, g dbSNP:113613489
508 508 a, g dbSNP:756866342
514 514 a, g dbSNP:373225799
524 524 a, g dbSNP:745394027
525 525 c, g dbSNP:769396929
538 538 a, g dbSNP:17850721
553 553 c, t dbSNP:779679844
556 556 a, g dbSNP:543352858
581 581 c, g dbSNP:768655922
592 592 c, t dbSNP:774293070
595 595 c, t dbSNP:376539678
614 614 a, g dbSNP:771079572
623 623 g, t dbSNP:776957366
627 627 a, g, t dbSNP:139780199
628 628 c, t dbSNP:200828715
635 635 a, g dbSNP:775530911
637 637 c, g dbSNP:763468580
646 646 c, t dbSNP:748181994
647 647 a, t dbSNP:144503053
658 658 a, g dbSNP:146607451
660 660 c, g dbSNP:746719599
667 667 c, t dbSNP:770038359
696 696 c, g dbSNP:780203550
700 700 c, t dbSNP:749491356
702 702 c, g dbSNP:768764962
706 706 a, c, g dbSNP:149180222
708 708 c, t dbSNP:772391489
709 709 g, t dbSNP:368583920
746 746 a, g dbSNP:373486719
748 748 c, t dbSNP:766826666
763 763 a, g dbSNP:180973800
765 765 c, t dbSNP:549498464
773 773 a, g dbSNP:113912958
798 798 a, g dbSNP:373676771
799 799 c, t dbSNP:752087000
809 809 c, g dbSNP:758364307
817 817 a, g dbSNP:777534174
822 822 a, g dbSNP:751396536
826 826 a, g dbSNP:187368856
830 830 a, g dbSNP:780934823
834 834 a, g dbSNP:749579216
843 843 g, t dbSNP:768854709
847 847 a, g dbSNP:779064587
849 849 a, c dbSNP:748136044
861 861 a, g dbSNP:561386917
871 871 c, t dbSNP:750161964
872 872 c, g dbSNP:755258124
876 876 a, g dbSNP:778951638
887 887 g, t dbSNP:748380323
916 916 c, t dbSNP:143296563
917 917 a, g dbSNP:777901369
920 920 c, t dbSNP:747572749
922 922 a, g dbSNP:771505562
931 931 c, t dbSNP:777136708
933 933 a, g dbSNP:201795443
941 941 a, g dbSNP:769511426
944 944 a, g dbSNP:775059137
951 951 a, t dbSNP:762640222
956 956 c, t dbSNP:763579465
984 984 a, g dbSNP:550279324
1014 1014 a, g dbSNP:773837230
1020 1020 c, g dbSNP:71329463
1021 1021 c, t dbSNP:761753616
1031 1031 c, g dbSNP:765406792
1038 1038 a, c dbSNP:151266236
1041 1041 c, g dbSNP:766375927
1054 1054 c, t dbSNP:757463604
1059 1059 a, g dbSNP:201549256
1064 1064 c, t dbSNP:750961802
1074 1074 a, c dbSNP:762126832
1076 1076 a, g dbSNP:568113525
1088 1088 c, g dbSNP:750461208
1095 1095 c, t dbSNP:756584765
1098 1098 a, g dbSNP:780572230
1110 1110 a, c dbSNP:755463134
1112 1112 g, t dbSNP:541906192
1114 1114 a, g dbSNP:752989102
1142 1142 g, t dbSNP:758074601
1145 1145 a, g dbSNP:777431811
1153 1153 a, g dbSNP:746617487
1165 1165 c, t dbSNP:370548139
1170 1170 a, g, t dbSNP:749141828
1171 1171 c, t dbSNP:745862670
1176 1176 a, c dbSNP:769800257
1182 1182 a, g dbSNP:775430263
1201 1201 a, t dbSNP:749052439
1204 1204 c, g dbSNP:772207979
1206 1206 a, g dbSNP:773521736
1207 1207 a, g dbSNP:760801544
1213 1213 g, t dbSNP:369419016
1215 1215 c, t dbSNP:766538628
1217 1217 a, g dbSNP:776515950
1226 1226 a, t dbSNP:759560086
1230 1230 a, g dbSNP:769840252
1234 1234 a, g dbSNP:189488022
1235 1235 a, g dbSNP:763368047
1241 1241 a, c dbSNP:764581545
1247 1247 a, g dbSNP:751816550
1256 1256 a, c dbSNP:749359827
1260 1260 a, g dbSNP:761535308
1283 1283 c, g dbSNP:767038055
1291 1291 c, t dbSNP:376649965
1292 1292 a, g dbSNP:755735886
1294 1294 c, t dbSNP:779704391
1303 1303 a, g dbSNP:753883803
1305 1305 c, t dbSNP:754812668
1306 1306 c, t dbSNP:778774026
1316 1316 c, g dbSNP:747853630
1322 1322 a, g dbSNP:200360700
1324 1324 c, t dbSNP:571122134
1325 1325 a, g dbSNP:779579241
1334 1334 g, t dbSNP:781439045
1336 1336 c, t dbSNP:748602761
1337 1337 a, g dbSNP:538447999
1354 1354 g, t dbSNP:776226649
1355 1355 a, c dbSNP:759017252
1363 1363 c, t dbSNP:764772705
1371 1371 c, g dbSNP:752642647
1376 1376 c, g dbSNP:758320166
1377 1377 -, t dbSNP:761072260
1384 1384 a, c, t dbSNP:763891431
1395 1395 a, g dbSNP:200670756
1401 1401 a, c dbSNP:780205458
1402 1402 g, t dbSNP:749508131
1409 1409 a, g dbSNP:201802087
1418 1418 a, g dbSNP:779048302
1419 1419 c, t dbSNP:775029900
1421 1421 a, c dbSNP:772636858
1430 1430 c, g dbSNP:773739451
1431 1431 c, t dbSNP:747325080
1432 1432 a, g dbSNP:771399567
1435 1435 a, c dbSNP:776186420
1439 1439 c, t dbSNP:759221648
1440 1440 a, g dbSNP:764710364
1451 1451 a, g dbSNP:41311143
1454 1454 a, g dbSNP:762883675
1455 1455 -, gcc dbSNP:766881855
1469 1469 c, t dbSNP:138287627
1470 1470 a, g dbSNP:751377720
1477 1477 -, ccaggaggc dbSNP:779863218
1478 1478 -, ccaggaggc dbSNP:755863352
1486 1486 c, t dbSNP:568182920
1495 1495 c, t dbSNP:752168245
1498 1498 g, t dbSNP:757722744
1505 1505 c, t dbSNP:781417091
1507 1507 c, g dbSNP:746205642
1508 1508 a, g dbSNP:143781060
1510 1510 a, g dbSNP:779806745
1511 1511 a, g dbSNP:371203156
1515 1515 a, g dbSNP:768226495
1521 1521 c, g dbSNP:773841106
1525 1525 c, g dbSNP:761755379
1526 1526 c, t dbSNP:772003903
1527 1527 a, g dbSNP:146069416
1529 1529 -, gga dbSNP:748914699
1529 1529 a, g dbSNP:760562435
1546 1546 c, t dbSNP:766057311
1561 1561 a, g dbSNP:753034592
1564 1564 c, t dbSNP:763253549
1566 1566 a, g dbSNP:764280754
1575 1575 a, g, t dbSNP:751624817
1576 1576 a, c dbSNP:781689077
1578 1578 a, g dbSNP:750873132
1583 1583 a, c, t dbSNP:756545471
1588 1588 c, g, t dbSNP:749000594
1591 1591 a, g dbSNP:778423646
1593 1593 c, g dbSNP:747642485
1594 1594 a, t dbSNP:375087308
1601 1601 a, g dbSNP:535564625
1608 1608 a, c dbSNP:746916052
1627 1627 a, g dbSNP:770816500
1633 1633 c, g dbSNP:776510331
1638 1638 c, t dbSNP:371101669
1639 1639 c, t dbSNP:764299687
1640 1640 g, t dbSNP:769690646
1643 1643 a, g dbSNP:774755302
1646 1646 a, g dbSNP:199700003
1649 1649 c, g dbSNP:371363660
1662 1662 -, tgtaat dbSNP:777195893
1670 1670 c, g dbSNP:773111625
1674 1674 a, g dbSNP:761252899
1695 1695 a, g dbSNP:201950459
1699 1699 c, t dbSNP:138837921
1711 1711 a, g dbSNP:374886351
1735 1735 a, g dbSNP:754318835
1746 1746 c, g dbSNP:755371218
1750 1750 c, t dbSNP:765577876
1755 1755 c, t dbSNP:149288880
1756 1756 a, g dbSNP:144536392
1765 1765 c, t dbSNP:369377914
1767 1767 c, t dbSNP:372053944
1768 1768 a, g dbSNP:757284121
1771 1771 c, t dbSNP:373600548
1773 1773 c, t dbSNP:745768944
1784 1784 c, g, t dbSNP:199957418
1785 1785 a, g dbSNP:747223579
1803 1803 c, g dbSNP:771052631
1812 1812 a, g dbSNP:776633985
1814 1814 g, t dbSNP:760040574
1822 1822 a, g dbSNP:770158019
1838 1838 c, t dbSNP:375159283
1839 1839 a, g dbSNP:763277358
1849 1849 c, t dbSNP:148438243
1850 1850 a, g dbSNP:751324003
1856 1856 a, g dbSNP:761544613
1861 1861 a, c, g dbSNP:368221410
1878 1878 g, t dbSNP:756154356
1888 1888 g, t dbSNP:766181979
1892 1892 c, t dbSNP:753775393
1895 1895 c, t dbSNP:754794055
1901 1901 a, g dbSNP:371220999
1903 1903 c, t dbSNP:575816889
1905 1905 a, g dbSNP:757403640
1908 1908 a, g dbSNP:368700556
1919 1919 a, g dbSNP:745897992
1921 1921 g, t dbSNP:769896639
1924 1924 c, t dbSNP:182236482
1933 1933 a, t dbSNP:564325903
1941 1941 a, g dbSNP:201365388
1942 1942 a, c dbSNP:749797583
1948 1948 c, g, t dbSNP:111270799
1949 1949 c, t dbSNP:774802216
1951 1951 a, t dbSNP:373731597
1959 1959 c, t dbSNP:767241430
1976 1976 g, t dbSNP:202103106
1980 1980 a, c dbSNP:200316952
1981 1981 a, g dbSNP:765905902
1982 1982 a, t dbSNP:540524061
1989 1989 -, gaa dbSNP:779809862
1989 1989 c, g dbSNP:754883032
1996 1996 a, g dbSNP:764945495
2004 2004 a, g dbSNP:752449468
2030 2030 a, g dbSNP:145953477
2032 2032 c, t dbSNP:769261609
2049 2049 a, g dbSNP:775473690
2052 2052 a, g dbSNP:762865145
2058 2058 a, g dbSNP:772073607
2059 2059 a, g, t dbSNP:763891504
2063 2063 -, actagatg dbSNP:747507384
2070 2070 a, t dbSNP:139786904
2072 2072 c, g dbSNP:760840445
2081 2081 c, t dbSNP:370708728
2082 2082 a, g dbSNP:200423555
2084 2084 a, g dbSNP:755153099
2087 2087 -, g dbSNP:777279508
2088 2088 c, t dbSNP:779118399
2093 2093 c, t dbSNP:368593909
2096 2096 a, g dbSNP:758905291
2098 2098 a, t dbSNP:778310719
2101 2101 a, c dbSNP:370466002
2118 2118 c, g dbSNP:747348720
2143 2143 a, t dbSNP:371953876
2164 2164 a, c dbSNP:3205130
2192 2192 c, t dbSNP:544683947
2197 2197 c, g dbSNP:728760
2210 2210 -, t dbSNP:532857025
2222 2222 c, g dbSNP:557978659
2232 2232 a, g dbSNP:758967536
2238 2238 a, g dbSNP:573094995
2248 2248 g, t dbSNP:540536395
2250 2250 a, g dbSNP:562200272
2263 2263 c, t dbSNP:11545666
2287 2287 -, taac dbSNP:551098529
2293 2293 c, t dbSNP:149241548
2296 2296 -, aaca dbSNP:370714754
2311 2311 c, t dbSNP:193121445
2355 2355 c, t dbSNP:1055852
2385 2385 -, g dbSNP:537969245
2386 2386 -, t dbSNP:776760592
2390 2390 g, t dbSNP:528944830
2399 2399 -, ttt dbSNP:76631619
2401 2401 a, t dbSNP:551900901
2423 2423 c, t dbSNP:375258481
2427 2427 c, g dbSNP:560429326

Target ORF information:

RefSeq Version XM_011529995
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529995

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TAAGGCCCCA
AAGCCTGAAG GCTTCCTCCC GCCACCCTTT CCGCCCCCGG GTGGTGATCG TGGCAGAGGT
GGCCCTGGTG GCATGCGGGG AGGAAGAGGT GGCCTCATGG ATCGTGGTGG TCCCGGTGGA
ATGTTCAGAG GTGGCCGTGG TGGAGACAGA GGTGGCTTCC GTGGTGGCCG GGGCATGGAC
CGAGGTGGCT TTGGTGGAGG AAGACGAGGT GGCCCTGGGG GGCCCCCTGG ACCTTTGATG
GAACAGATGG GAGGAAGAAG AGGAGGACGT GGAGGACCTG GAAAAATGGA TAAAGGCGAG
CACCGTCAGG AGCGCAGAGA TCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53446
Accession Version XM_005261389.2
Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530419639. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005261446.1
CDS84..2093
Misc Feature(1)1164..1415(+)
Misc Feature(2)1170..1409(+)
Misc Feature(3)1635..1763(+)
Misc Feature(4)1647..1751(+)
Misc Feature(5)1653..1751(+)
Translation
Position Chain Variation Link
27 27 c, g dbSNP:539670651
31 31 a, c dbSNP:551097984
33 33 c, g dbSNP:770561780
35 35 a, c, g dbSNP:758447329
36 36 a, c dbSNP:758975710
39 39 a, c, t dbSNP:111892587
42 42 a, g dbSNP:367933864
47 47 a, c dbSNP:763833724
49 49 a, g dbSNP:751395202
50 50 a, c, g dbSNP:756462778
51 51 a, g dbSNP:754003425
53 53 a, g dbSNP:754966628
55 55 -, g dbSNP:760330065
59 59 a, g dbSNP:199589576
63 63 a, t dbSNP:748647019
64 64 a, g dbSNP:758943413
65 65 c, g dbSNP:201886504
66 66 c, g, t dbSNP:377068270
67 67 a, g dbSNP:776222972
69 69 c, g dbSNP:745403052
75 75 a, g dbSNP:375095904
76 76 a, g dbSNP:552992152
80 80 c, g dbSNP:762864394
81 81 a, g dbSNP:2231392
90 90 a, t dbSNP:774293267
92 92 c, t dbSNP:779516758
93 93 a, c dbSNP:761661070
95 95 a, c, g dbSNP:147203739
105 105 a, g dbSNP:772125233
107 107 c, t dbSNP:201792944
109 109 a, g dbSNP:768830118
110 110 c, t dbSNP:140505486
117 117 a, g dbSNP:769936524
125 125 a, g dbSNP:775729620
131 131 a, g dbSNP:763137483
149 149 c, t dbSNP:377732811
150 150 a, g dbSNP:777722463
151 151 c, t dbSNP:746797640
152 152 c, t dbSNP:770806014
159 159 a, g, t dbSNP:776256103
186 186 a, g dbSNP:753624585
188 188 a, c, g dbSNP:141206174
202 202 a, g dbSNP:765008447
205 205 a, g dbSNP:752450196
212 212 c, g dbSNP:762485960
214 214 a, g dbSNP:374454766
215 215 c, t dbSNP:763729441
218 218 a, g dbSNP:751022322
241 241 c, g dbSNP:757233633
249 249 c, g dbSNP:781041301
251 251 c, g dbSNP:150734586
255 255 a, c dbSNP:755951683
263 263 c, t dbSNP:779793507
272 272 c, g, t dbSNP:748434313
273 273 a, t dbSNP:777919893
275 275 c, t dbSNP:573011438
280 280 a, g dbSNP:771496966
284 284 a, g dbSNP:139048680
285 285 a, g dbSNP:368705995
293 293 c, t dbSNP:770326364
296 296 a, g dbSNP:775996281
303 303 c, t dbSNP:762726444
306 306 a, g dbSNP:556275941
321 321 a, c dbSNP:778130614
324 324 a, g dbSNP:768951742
327 327 a, g dbSNP:182195687
329 329 a, c dbSNP:747812796
343 343 a, g dbSNP:377199824
350 350 c, t dbSNP:772711384
352 352 c, t dbSNP:760136386
355 355 a, c dbSNP:766322894
356 356 a, g dbSNP:776479146
357 357 c, g dbSNP:759489827
359 359 g, t dbSNP:765011656
376 376 -, atg dbSNP:769502605
382 382 a, c dbSNP:752526721
384 384 a, g dbSNP:757568499
387 387 a, g dbSNP:370033991
395 395 a, g, t dbSNP:750563135
398 398 -, cac dbSNP:775127785
398 398 a, c dbSNP:780159761
401 401 c, t dbSNP:749826758
408 408 c, g dbSNP:755496976
413 413 c, t dbSNP:779156750
414 414 g, t dbSNP:748442517
427 427 a, g dbSNP:771741224
434 434 a, g dbSNP:373180201
440 440 c, t dbSNP:746637617
442 442 c, g dbSNP:770384643
452 452 c, t dbSNP:776204033
455 455 c, t dbSNP:759453504
463 463 a, g dbSNP:559661693
467 467 c, g dbSNP:775155431
471 471 c, g dbSNP:762797076
475 475 a, c dbSNP:200282253
483 483 a, g dbSNP:750724221
487 487 c, t dbSNP:760949110
492 492 a, g dbSNP:149885670
495 495 a, c dbSNP:753897217
499 499 c, g, t dbSNP:765207539
500 500 a, c, g dbSNP:758898123
512 512 c, g dbSNP:200637771
518 518 c, t dbSNP:146418156
532 532 a, g dbSNP:113613489
533 533 a, g dbSNP:756866342
539 539 a, g dbSNP:373225799
549 549 a, g dbSNP:745394027
550 550 c, g dbSNP:769396929
563 563 a, g dbSNP:17850721
578 578 c, t dbSNP:779679844
581 581 a, g dbSNP:543352858
606 606 c, g dbSNP:768655922
617 617 c, t dbSNP:774293070
620 620 c, t dbSNP:376539678
639 639 a, g dbSNP:771079572
648 648 g, t dbSNP:776957366
652 652 a, g, t dbSNP:139780199
653 653 c, t dbSNP:200828715
660 660 a, g dbSNP:775530911
662 662 c, g dbSNP:763468580
671 671 c, t dbSNP:748181994
672 672 a, t dbSNP:144503053
683 683 a, g dbSNP:146607451
685 685 c, g dbSNP:746719599
692 692 c, t dbSNP:770038359
721 721 c, g dbSNP:780203550
725 725 c, t dbSNP:749491356
727 727 c, g dbSNP:768764962
731 731 a, c, g dbSNP:149180222
733 733 c, t dbSNP:772391489
734 734 g, t dbSNP:368583920
771 771 a, g dbSNP:373486719
773 773 c, t dbSNP:766826666
788 788 a, g dbSNP:180973800
790 790 c, t dbSNP:549498464
798 798 a, g dbSNP:113912958
823 823 a, g dbSNP:373676771
824 824 c, t dbSNP:752087000
834 834 c, g dbSNP:758364307
842 842 a, g dbSNP:777534174
847 847 a, g dbSNP:751396536
851 851 a, g dbSNP:187368856
855 855 a, g dbSNP:780934823
859 859 a, g dbSNP:749579216
868 868 g, t dbSNP:768854709
872 872 a, g dbSNP:779064587
874 874 a, c dbSNP:748136044
886 886 a, g dbSNP:561386917
896 896 c, t dbSNP:750161964
897 897 c, g dbSNP:755258124
901 901 a, g dbSNP:778951638
912 912 g, t dbSNP:748380323
941 941 c, t dbSNP:143296563
942 942 a, g dbSNP:777901369
945 945 c, t dbSNP:747572749
947 947 a, g dbSNP:771505562
956 956 c, t dbSNP:777136708
958 958 a, g dbSNP:201795443
966 966 a, g dbSNP:769511426
969 969 a, g dbSNP:775059137
976 976 a, t dbSNP:762640222
981 981 c, t dbSNP:763579465
1009 1009 a, g dbSNP:550279324
1039 1039 a, g dbSNP:773837230
1045 1045 c, g dbSNP:71329463
1046 1046 c, t dbSNP:761753616
1056 1056 c, g dbSNP:765406792
1063 1063 a, c dbSNP:151266236
1066 1066 c, g dbSNP:766375927
1079 1079 c, t dbSNP:757463604
1084 1084 a, g dbSNP:201549256
1089 1089 c, t dbSNP:750961802
1099 1099 a, c dbSNP:762126832
1101 1101 a, g dbSNP:568113525
1113 1113 c, g dbSNP:750461208
1120 1120 c, t dbSNP:756584765
1123 1123 a, g dbSNP:780572230
1135 1135 a, c dbSNP:755463134
1137 1137 g, t dbSNP:541906192
1139 1139 a, g dbSNP:752989102
1167 1167 g, t dbSNP:758074601
1170 1170 a, g dbSNP:777431811
1178 1178 a, g dbSNP:746617487
1190 1190 c, t dbSNP:370548139
1195 1195 a, g, t dbSNP:749141828
1196 1196 c, t dbSNP:745862670
1201 1201 a, c dbSNP:769800257
1207 1207 a, g dbSNP:775430263
1226 1226 a, t dbSNP:749052439
1229 1229 c, g dbSNP:772207979
1231 1231 a, g dbSNP:773521736
1232 1232 a, g dbSNP:760801544
1238 1238 g, t dbSNP:369419016
1240 1240 c, t dbSNP:766538628
1242 1242 a, g dbSNP:776515950
1251 1251 a, t dbSNP:759560086
1255 1255 a, g dbSNP:769840252
1259 1259 a, g dbSNP:189488022
1260 1260 a, g dbSNP:763368047
1266 1266 a, c dbSNP:764581545
1272 1272 a, g dbSNP:751816550
1281 1281 a, c dbSNP:749359827
1285 1285 a, g dbSNP:761535308
1308 1308 c, g dbSNP:767038055
1316 1316 c, t dbSNP:376649965
1317 1317 a, g dbSNP:755735886
1319 1319 c, t dbSNP:779704391
1328 1328 a, g dbSNP:753883803
1330 1330 c, t dbSNP:754812668
1331 1331 c, t dbSNP:778774026
1341 1341 c, g dbSNP:747853630
1347 1347 a, g dbSNP:200360700
1349 1349 c, t dbSNP:571122134
1350 1350 a, g dbSNP:779579241
1359 1359 g, t dbSNP:781439045
1361 1361 c, t dbSNP:748602761
1362 1362 a, g dbSNP:538447999
1379 1379 g, t dbSNP:776226649
1380 1380 a, c dbSNP:759017252
1388 1388 c, t dbSNP:764772705
1396 1396 c, g dbSNP:752642647
1401 1401 c, g dbSNP:758320166
1402 1402 -, t dbSNP:761072260
1409 1409 a, c, t dbSNP:763891431
1420 1420 a, g dbSNP:200670756
1426 1426 a, c dbSNP:780205458
1427 1427 g, t dbSNP:749508131
1434 1434 a, g dbSNP:201802087
1443 1443 a, g dbSNP:779048302
1444 1444 c, t dbSNP:775029900
1446 1446 a, c dbSNP:772636858
1455 1455 c, g dbSNP:773739451
1456 1456 c, t dbSNP:747325080
1457 1457 a, g dbSNP:771399567
1460 1460 a, c dbSNP:776186420
1464 1464 c, t dbSNP:759221648
1465 1465 a, g dbSNP:764710364
1476 1476 a, g dbSNP:41311143
1479 1479 a, g dbSNP:762883675
1480 1480 -, gcc dbSNP:766881855
1494 1494 c, t dbSNP:138287627
1495 1495 a, g dbSNP:751377720
1502 1502 -, ccaggaggc dbSNP:779863218
1503 1503 -, ccaggaggc dbSNP:755863352
1511 1511 c, t dbSNP:568182920
1520 1520 c, t dbSNP:752168245
1523 1523 g, t dbSNP:757722744
1530 1530 c, t dbSNP:781417091
1532 1532 c, g dbSNP:746205642
1533 1533 a, g dbSNP:143781060
1535 1535 a, g dbSNP:779806745
1536 1536 a, g dbSNP:371203156
1540 1540 a, g dbSNP:768226495
1546 1546 c, g dbSNP:773841106
1550 1550 c, g dbSNP:761755379
1551 1551 c, t dbSNP:772003903
1552 1552 a, g dbSNP:146069416
1554 1554 -, gga dbSNP:748914699
1554 1554 a, g dbSNP:760562435
1571 1571 c, t dbSNP:766057311
1586 1586 a, g dbSNP:753034592
1589 1589 c, t dbSNP:763253549
1591 1591 a, g dbSNP:764280754
1600 1600 a, g, t dbSNP:751624817
1601 1601 a, c dbSNP:781689077
1603 1603 a, g dbSNP:750873132
1608 1608 a, c, t dbSNP:756545471
1613 1613 c, g, t dbSNP:749000594
1616 1616 a, g dbSNP:778423646
1618 1618 c, g dbSNP:747642485
1619 1619 a, t dbSNP:375087308
1626 1626 a, g dbSNP:535564625
1633 1633 a, c dbSNP:746916052
1652 1652 a, g dbSNP:770816500
1658 1658 c, g dbSNP:776510331
1663 1663 c, t dbSNP:371101669
1664 1664 c, t dbSNP:764299687
1665 1665 g, t dbSNP:769690646
1668 1668 a, g dbSNP:774755302
1671 1671 a, g dbSNP:199700003
1674 1674 c, g dbSNP:371363660
1687 1687 -, tgtaat dbSNP:777195893
1695 1695 c, g dbSNP:773111625
1699 1699 a, g dbSNP:761252899
1720 1720 a, g dbSNP:201950459
1724 1724 c, t dbSNP:138837921
1736 1736 a, g dbSNP:374886351
1760 1760 a, g dbSNP:754318835
1771 1771 c, g dbSNP:755371218
1775 1775 c, t dbSNP:765577876
1780 1780 c, t dbSNP:149288880
1781 1781 a, g dbSNP:144536392
1790 1790 c, t dbSNP:369377914
1792 1792 c, t dbSNP:372053944
1793 1793 a, g dbSNP:757284121
1796 1796 c, t dbSNP:373600548
1798 1798 c, t dbSNP:745768944
1809 1809 c, g, t dbSNP:199957418
1810 1810 a, g dbSNP:747223579
1828 1828 c, g dbSNP:771052631
1837 1837 a, g dbSNP:776633985
1839 1839 g, t dbSNP:760040574
1847 1847 a, g dbSNP:770158019
1863 1863 c, t dbSNP:375159283
1864 1864 a, g dbSNP:763277358
1874 1874 c, t dbSNP:148438243
1875 1875 a, g dbSNP:751324003
1881 1881 a, g dbSNP:761544613
1886 1886 a, c, g dbSNP:368221410
1903 1903 g, t dbSNP:756154356
1913 1913 g, t dbSNP:766181979
1917 1917 c, t dbSNP:753775393
1920 1920 c, t dbSNP:754794055
1926 1926 a, g dbSNP:371220999
1928 1928 c, t dbSNP:575816889
1930 1930 a, g dbSNP:757403640
1933 1933 a, g dbSNP:368700556
1944 1944 a, g dbSNP:745897992
1946 1946 g, t dbSNP:769896639
1949 1949 c, t dbSNP:182236482
1958 1958 a, t dbSNP:564325903
1966 1966 a, g dbSNP:201365388
1967 1967 a, c dbSNP:749797583
1973 1973 c, g, t dbSNP:111270799
1974 1974 c, t dbSNP:774802216
1976 1976 a, t dbSNP:373731597
1984 1984 c, t dbSNP:767241430
2001 2001 g, t dbSNP:202103106
2005 2005 a, c dbSNP:200316952
2006 2006 a, g dbSNP:765905902
2007 2007 a, t dbSNP:540524061
2014 2014 -, gaa dbSNP:779809862
2014 2014 c, g dbSNP:754883032
2021 2021 a, g dbSNP:764945495
2029 2029 a, g dbSNP:752449468
2055 2055 a, g dbSNP:145953477
2057 2057 c, t dbSNP:769261609
2074 2074 a, g dbSNP:775473690
2077 2077 a, g dbSNP:762865145
2083 2083 a, g dbSNP:772073607
2084 2084 a, g, t dbSNP:763891504
2088 2088 -, actagatg dbSNP:747507384
2095 2095 a, t dbSNP:139786904
2097 2097 c, g dbSNP:760840445
2106 2106 c, t dbSNP:370708728
2107 2107 a, g dbSNP:200423555
2109 2109 a, g dbSNP:755153099
2112 2112 -, g dbSNP:777279508
2113 2113 c, t dbSNP:779118399
2118 2118 c, t dbSNP:368593909
2121 2121 a, g dbSNP:758905291
2123 2123 a, t dbSNP:778310719
2126 2126 a, c dbSNP:370466002
2143 2143 c, g dbSNP:747348720
2168 2168 a, t dbSNP:371953876
2189 2189 a, c dbSNP:3205130
2217 2217 c, t dbSNP:544683947
2222 2222 c, g dbSNP:728760
2235 2235 -, t dbSNP:532857025
2247 2247 c, g dbSNP:557978659
2257 2257 a, g dbSNP:758967536
2263 2263 a, g dbSNP:573094995
2273 2273 g, t dbSNP:540536395
2275 2275 a, g dbSNP:562200272
2288 2288 c, t dbSNP:11545666
2312 2312 -, taac dbSNP:551098529
2318 2318 c, t dbSNP:149241548
2321 2321 -, aaca dbSNP:370714754
2336 2336 c, t dbSNP:193121445
2380 2380 c, t dbSNP:1055852
2410 2410 -, g dbSNP:537969245
2411 2411 -, t dbSNP:776760592
2415 2415 g, t dbSNP:528944830
2424 2424 -, ttt dbSNP:76631619
2426 2426 a, t dbSNP:551900901
2448 2448 c, t dbSNP:375258481
2452 2452 c, g dbSNP:560429326

Target ORF information:

RefSeq Version XM_005261389
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005261389

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57613
Accession Version XM_011529996.1
Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528298.1
CDS56..2065
Misc Feature(1)1136..1387(+)
Misc Feature(2)1142..1381(+)
Misc Feature(3)1607..1735(+)
Misc Feature(4)1619..1723(+)
Misc Feature(5)1625..1723(+)
Translation
Position Chain Variation Link
3 3 a, c dbSNP:551097984
5 5 c, g dbSNP:770561780
7 7 a, c, g dbSNP:758447329
8 8 a, c dbSNP:758975710
11 11 a, c, t dbSNP:111892587
14 14 a, g dbSNP:367933864
19 19 a, c dbSNP:763833724
21 21 a, g dbSNP:751395202
22 22 a, c, g dbSNP:756462778
23 23 a, g dbSNP:754003425
25 25 a, g dbSNP:754966628
27 27 -, g dbSNP:760330065
31 31 a, g dbSNP:199589576
35 35 a, t dbSNP:748647019
36 36 a, g dbSNP:758943413
37 37 c, g dbSNP:201886504
38 38 c, g, t dbSNP:377068270
39 39 a, g dbSNP:776222972
41 41 c, g dbSNP:745403052
47 47 a, g dbSNP:375095904
48 48 a, g dbSNP:552992152
52 52 c, g dbSNP:762864394
53 53 a, g dbSNP:2231392
62 62 a, t dbSNP:774293267
64 64 c, t dbSNP:779516758
65 65 a, c dbSNP:761661070
67 67 a, c, g dbSNP:147203739
77 77 a, g dbSNP:772125233
79 79 c, t dbSNP:201792944
81 81 a, g dbSNP:768830118
82 82 c, t dbSNP:140505486
89 89 a, g dbSNP:769936524
97 97 a, g dbSNP:775729620
103 103 a, g dbSNP:763137483
121 121 c, t dbSNP:377732811
122 122 a, g dbSNP:777722463
123 123 c, t dbSNP:746797640
124 124 c, t dbSNP:770806014
131 131 a, g, t dbSNP:776256103
161 161 a, g dbSNP:753624585
163 163 a, c, g dbSNP:141206174
177 177 a, g dbSNP:765008447
180 180 a, g dbSNP:752450196
187 187 c, g dbSNP:762485960
189 189 a, g dbSNP:374454766
190 190 c, t dbSNP:763729441
193 193 a, g dbSNP:751022322
216 216 c, g dbSNP:757233633
224 224 c, g dbSNP:781041301
226 226 c, g dbSNP:150734586
230 230 a, c dbSNP:755951683
238 238 c, t dbSNP:779793507
247 247 c, g, t dbSNP:748434313
248 248 a, t dbSNP:777919893
250 250 c, t dbSNP:573011438
255 255 a, g dbSNP:771496966
259 259 a, g dbSNP:139048680
260 260 a, g dbSNP:368705995
268 268 c, t dbSNP:770326364
271 271 a, g dbSNP:775996281
278 278 c, t dbSNP:762726444
281 281 a, g dbSNP:556275941
296 296 a, c dbSNP:778130614
299 299 a, g dbSNP:768951742
302 302 a, g dbSNP:182195687
304 304 a, c dbSNP:747812796
318 318 a, g dbSNP:377199824
325 325 c, t dbSNP:772711384
327 327 c, t dbSNP:760136386
330 330 a, c dbSNP:766322894
331 331 a, g dbSNP:776479146
332 332 c, g dbSNP:759489827
334 334 g, t dbSNP:765011656
351 351 -, atg dbSNP:769502605
357 357 a, c dbSNP:752526721
359 359 a, g dbSNP:757568499
362 362 a, g dbSNP:370033991
370 370 a, g, t dbSNP:750563135
373 373 -, cac dbSNP:775127785
373 373 a, c dbSNP:780159761
376 376 c, t dbSNP:749826758
383 383 c, g dbSNP:755496976
388 388 c, t dbSNP:779156750
389 389 g, t dbSNP:748442517
402 402 a, g dbSNP:771741224
409 409 a, g dbSNP:373180201
415 415 c, t dbSNP:746637617
417 417 c, g dbSNP:770384643
427 427 c, t dbSNP:776204033
430 430 c, t dbSNP:759453504
438 438 a, g dbSNP:559661693
442 442 c, g dbSNP:775155431
446 446 c, g dbSNP:762797076
450 450 a, c dbSNP:200282253
458 458 a, g dbSNP:750724221
462 462 c, t dbSNP:760949110
467 467 a, g dbSNP:149885670
470 470 a, c dbSNP:753897217
474 474 c, g, t dbSNP:765207539
475 475 a, c, g dbSNP:758898123
487 487 c, g dbSNP:200637771
493 493 c, t dbSNP:146418156
507 507 a, g dbSNP:113613489
508 508 a, g dbSNP:756866342
514 514 a, g dbSNP:373225799
524 524 a, g dbSNP:745394027
525 525 c, g dbSNP:769396929
538 538 a, g dbSNP:17850721
553 553 c, t dbSNP:779679844
556 556 a, g dbSNP:543352858
581 581 c, g dbSNP:768655922
592 592 c, t dbSNP:774293070
595 595 c, t dbSNP:376539678
614 614 a, g dbSNP:771079572
623 623 g, t dbSNP:776957366
627 627 a, g, t dbSNP:139780199
628 628 c, t dbSNP:200828715
635 635 a, g dbSNP:775530911
637 637 c, g dbSNP:763468580
646 646 c, t dbSNP:748181994
647 647 a, t dbSNP:144503053
658 658 a, g dbSNP:146607451
660 660 c, g dbSNP:746719599
667 667 c, t dbSNP:770038359
696 696 c, g dbSNP:780203550
700 700 c, t dbSNP:749491356
702 702 c, g dbSNP:768764962
706 706 a, c, g dbSNP:149180222
708 708 c, t dbSNP:772391489
709 709 g, t dbSNP:368583920
746 746 a, g dbSNP:373486719
748 748 c, t dbSNP:766826666
763 763 a, g dbSNP:180973800
765 765 c, t dbSNP:549498464
773 773 a, g dbSNP:113912958
798 798 a, g dbSNP:373676771
799 799 c, t dbSNP:752087000
809 809 c, g dbSNP:758364307
817 817 a, g dbSNP:777534174
822 822 a, g dbSNP:751396536
826 826 a, g dbSNP:187368856
830 830 a, g dbSNP:780934823
834 834 a, g dbSNP:749579216
843 843 g, t dbSNP:768854709
847 847 a, g dbSNP:779064587
849 849 a, c dbSNP:748136044
861 861 a, g dbSNP:561386917
871 871 c, t dbSNP:750161964
872 872 c, g dbSNP:755258124
876 876 a, g dbSNP:778951638
887 887 g, t dbSNP:748380323
916 916 c, t dbSNP:143296563
917 917 a, g dbSNP:777901369
920 920 c, t dbSNP:747572749
922 922 a, g dbSNP:771505562
931 931 c, t dbSNP:777136708
933 933 a, g dbSNP:201795443
941 941 a, g dbSNP:769511426
944 944 a, g dbSNP:775059137
951 951 a, t dbSNP:762640222
956 956 c, t dbSNP:763579465
984 984 a, g dbSNP:550279324
1014 1014 a, g dbSNP:773837230
1020 1020 c, g dbSNP:71329463
1021 1021 c, t dbSNP:761753616
1031 1031 c, g dbSNP:765406792
1035 1035 a, c dbSNP:151266236
1038 1038 c, g dbSNP:766375927
1051 1051 c, t dbSNP:757463604
1056 1056 a, g dbSNP:201549256
1061 1061 c, t dbSNP:750961802
1071 1071 a, c dbSNP:762126832
1073 1073 a, g dbSNP:568113525
1085 1085 c, g dbSNP:750461208
1092 1092 c, t dbSNP:756584765
1095 1095 a, g dbSNP:780572230
1107 1107 a, c dbSNP:755463134
1109 1109 g, t dbSNP:541906192
1111 1111 a, g dbSNP:752989102
1139 1139 g, t dbSNP:758074601
1142 1142 a, g dbSNP:777431811
1150 1150 a, g dbSNP:746617487
1162 1162 c, t dbSNP:370548139
1167 1167 a, g, t dbSNP:749141828
1168 1168 c, t dbSNP:745862670
1173 1173 a, c dbSNP:769800257
1179 1179 a, g dbSNP:775430263
1198 1198 a, t dbSNP:749052439
1201 1201 c, g dbSNP:772207979
1203 1203 a, g dbSNP:773521736
1204 1204 a, g dbSNP:760801544
1210 1210 g, t dbSNP:369419016
1212 1212 c, t dbSNP:766538628
1214 1214 a, g dbSNP:776515950
1223 1223 a, t dbSNP:759560086
1227 1227 a, g dbSNP:769840252
1231 1231 a, g dbSNP:189488022
1232 1232 a, g dbSNP:763368047
1238 1238 a, c dbSNP:764581545
1244 1244 a, g dbSNP:751816550
1253 1253 a, c dbSNP:749359827
1257 1257 a, g dbSNP:761535308
1280 1280 c, g dbSNP:767038055
1288 1288 c, t dbSNP:376649965
1289 1289 a, g dbSNP:755735886
1291 1291 c, t dbSNP:779704391
1300 1300 a, g dbSNP:753883803
1302 1302 c, t dbSNP:754812668
1303 1303 c, t dbSNP:778774026
1313 1313 c, g dbSNP:747853630
1319 1319 a, g dbSNP:200360700
1321 1321 c, t dbSNP:571122134
1322 1322 a, g dbSNP:779579241
1331 1331 g, t dbSNP:781439045
1333 1333 c, t dbSNP:748602761
1334 1334 a, g dbSNP:538447999
1351 1351 g, t dbSNP:776226649
1352 1352 a, c dbSNP:759017252
1360 1360 c, t dbSNP:764772705
1368 1368 c, g dbSNP:752642647
1373 1373 c, g dbSNP:758320166
1374 1374 -, t dbSNP:761072260
1381 1381 a, c, t dbSNP:763891431
1392 1392 a, g dbSNP:200670756
1398 1398 a, c dbSNP:780205458
1399 1399 g, t dbSNP:749508131
1406 1406 a, g dbSNP:201802087
1415 1415 a, g dbSNP:779048302
1416 1416 c, t dbSNP:775029900
1418 1418 a, c dbSNP:772636858
1427 1427 c, g dbSNP:773739451
1428 1428 c, t dbSNP:747325080
1429 1429 a, g dbSNP:771399567
1432 1432 a, c dbSNP:776186420
1436 1436 c, t dbSNP:759221648
1437 1437 a, g dbSNP:764710364
1448 1448 a, g dbSNP:41311143
1451 1451 a, g dbSNP:762883675
1452 1452 -, gcc dbSNP:766881855
1466 1466 c, t dbSNP:138287627
1467 1467 a, g dbSNP:751377720
1474 1474 -, ccaggaggc dbSNP:779863218
1475 1475 -, ccaggaggc dbSNP:755863352
1483 1483 c, t dbSNP:568182920
1492 1492 c, t dbSNP:752168245
1495 1495 g, t dbSNP:757722744
1502 1502 c, t dbSNP:781417091
1504 1504 c, g dbSNP:746205642
1505 1505 a, g dbSNP:143781060
1507 1507 a, g dbSNP:779806745
1508 1508 a, g dbSNP:371203156
1512 1512 a, g dbSNP:768226495
1518 1518 c, g dbSNP:773841106
1522 1522 c, g dbSNP:761755379
1523 1523 c, t dbSNP:772003903
1524 1524 a, g dbSNP:146069416
1526 1526 -, gga dbSNP:748914699
1526 1526 a, g dbSNP:760562435
1543 1543 c, t dbSNP:766057311
1558 1558 a, g dbSNP:753034592
1561 1561 c, t dbSNP:763253549
1563 1563 a, g dbSNP:764280754
1572 1572 a, g, t dbSNP:751624817
1573 1573 a, c dbSNP:781689077
1575 1575 a, g dbSNP:750873132
1580 1580 a, c, t dbSNP:756545471
1585 1585 c, g, t dbSNP:749000594
1588 1588 a, g dbSNP:778423646
1590 1590 c, g dbSNP:747642485
1591 1591 a, t dbSNP:375087308
1598 1598 a, g dbSNP:535564625
1605 1605 a, c dbSNP:746916052
1624 1624 a, g dbSNP:770816500
1630 1630 c, g dbSNP:776510331
1635 1635 c, t dbSNP:371101669
1636 1636 c, t dbSNP:764299687
1637 1637 g, t dbSNP:769690646
1640 1640 a, g dbSNP:774755302
1643 1643 a, g dbSNP:199700003
1646 1646 c, g dbSNP:371363660
1659 1659 -, tgtaat dbSNP:777195893
1667 1667 c, g dbSNP:773111625
1671 1671 a, g dbSNP:761252899
1692 1692 a, g dbSNP:201950459
1696 1696 c, t dbSNP:138837921
1708 1708 a, g dbSNP:374886351
1732 1732 a, g dbSNP:754318835
1743 1743 c, g dbSNP:755371218
1747 1747 c, t dbSNP:765577876
1752 1752 c, t dbSNP:149288880
1753 1753 a, g dbSNP:144536392
1762 1762 c, t dbSNP:369377914
1764 1764 c, t dbSNP:372053944
1765 1765 a, g dbSNP:757284121
1768 1768 c, t dbSNP:373600548
1770 1770 c, t dbSNP:745768944
1781 1781 c, g, t dbSNP:199957418
1782 1782 a, g dbSNP:747223579
1800 1800 c, g dbSNP:771052631
1809 1809 a, g dbSNP:776633985
1811 1811 g, t dbSNP:760040574
1819 1819 a, g dbSNP:770158019
1835 1835 c, t dbSNP:375159283
1836 1836 a, g dbSNP:763277358
1846 1846 c, t dbSNP:148438243
1847 1847 a, g dbSNP:751324003
1853 1853 a, g dbSNP:761544613
1858 1858 a, c, g dbSNP:368221410
1875 1875 g, t dbSNP:756154356
1885 1885 g, t dbSNP:766181979
1889 1889 c, t dbSNP:753775393
1892 1892 c, t dbSNP:754794055
1898 1898 a, g dbSNP:371220999
1900 1900 c, t dbSNP:575816889
1902 1902 a, g dbSNP:757403640
1905 1905 a, g dbSNP:368700556
1916 1916 a, g dbSNP:745897992
1918 1918 g, t dbSNP:769896639
1921 1921 c, t dbSNP:182236482
1930 1930 a, t dbSNP:564325903
1938 1938 a, g dbSNP:201365388
1939 1939 a, c dbSNP:749797583
1945 1945 c, g, t dbSNP:111270799
1946 1946 c, t dbSNP:774802216
1948 1948 a, t dbSNP:373731597
1956 1956 c, t dbSNP:767241430
1973 1973 g, t dbSNP:202103106
1977 1977 a, c dbSNP:200316952
1978 1978 a, g dbSNP:765905902
1979 1979 a, t dbSNP:540524061
1986 1986 -, gaa dbSNP:779809862
1986 1986 c, g dbSNP:754883032
1993 1993 a, g dbSNP:764945495
2001 2001 a, g dbSNP:752449468
2027 2027 a, g dbSNP:145953477
2029 2029 c, t dbSNP:769261609
2046 2046 a, g dbSNP:775473690
2049 2049 a, g dbSNP:762865145
2055 2055 a, g dbSNP:772073607
2056 2056 a, g, t dbSNP:763891504
2060 2060 -, actagatg dbSNP:747507384
2067 2067 a, t dbSNP:139786904
2069 2069 c, g dbSNP:760840445
2078 2078 c, t dbSNP:370708728
2079 2079 a, g dbSNP:200423555
2081 2081 a, g dbSNP:755153099
2084 2084 -, g dbSNP:777279508
2085 2085 c, t dbSNP:779118399
2090 2090 c, t dbSNP:368593909
2093 2093 a, g dbSNP:758905291
2095 2095 a, t dbSNP:778310719
2098 2098 a, c dbSNP:370466002
2115 2115 c, g dbSNP:747348720
2140 2140 a, t dbSNP:371953876
2161 2161 a, c dbSNP:3205130
2189 2189 c, t dbSNP:544683947
2194 2194 c, g dbSNP:728760
2207 2207 -, t dbSNP:532857025
2219 2219 c, g dbSNP:557978659
2229 2229 a, g dbSNP:758967536
2235 2235 a, g dbSNP:573094995
2245 2245 g, t dbSNP:540536395
2247 2247 a, g dbSNP:562200272
2260 2260 c, t dbSNP:11545666
2284 2284 -, taac dbSNP:551098529
2290 2290 c, t dbSNP:149241548
2293 2293 -, aaca dbSNP:370714754
2308 2308 c, t dbSNP:193121445
2352 2352 c, t dbSNP:1055852
2382 2382 -, g dbSNP:537969245
2383 2383 -, t dbSNP:776760592
2387 2387 g, t dbSNP:528944830
2396 2396 -, ttt dbSNP:76631619
2398 2398 a, t dbSNP:551900901
2420 2420 c, t dbSNP:375258481
2424 2424 c, g dbSNP:560429326

Target ORF information:

RefSeq Version XM_011529996
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529996

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57614
Accession Version XM_011529997.1
Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CGCCACCCTT TCCGCCCCCG
GGTGGTGATC GTGGCAGAGG TGGCCCTGGT GGCATGCGGG GAGGAAGAGG TGGCCTCATG
GATCGTGGTG GTCCCGGTGG AATGTTCAGA GGTGGCCGTG GTGGAGACAG AGGTGGCTTC
CGTGGTGGCC GGGGCATGGA CCGAGGTGGC TTTGGTGGAG GAAGACGAGG TGGCCCTGGG
GGGCCCCCTG GACCTTTGAT GGAACAGATG GGAGGAAGAA GAGGAGGACG TGGAGGACCT
GGAAAAATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ATCGGCCCTA CTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528299.1
CDS56..2029
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..1699(+)
Misc Feature(4)1622..1687(+)
Misc Feature(5)1628..1687(+)
Translation
Position Chain Variation Link
3 3 a, c dbSNP:551097984
5 5 c, g dbSNP:770561780
7 7 a, c, g dbSNP:758447329
8 8 a, c dbSNP:758975710
11 11 a, c, t dbSNP:111892587
14 14 a, g dbSNP:367933864
19 19 a, c dbSNP:763833724
21 21 a, g dbSNP:751395202
22 22 a, c, g dbSNP:756462778
23 23 a, g dbSNP:754003425
25 25 a, g dbSNP:754966628
27 27 -, g dbSNP:760330065
31 31 a, g dbSNP:199589576
35 35 a, t dbSNP:748647019
36 36 a, g dbSNP:758943413
37 37 c, g dbSNP:201886504
38 38 c, g, t dbSNP:377068270
39 39 a, g dbSNP:776222972
41 41 c, g dbSNP:745403052
47 47 a, g dbSNP:375095904
48 48 a, g dbSNP:552992152
52 52 c, g dbSNP:762864394
53 53 a, g dbSNP:2231392
62 62 a, t dbSNP:774293267
64 64 c, t dbSNP:779516758
65 65 a, c dbSNP:761661070
67 67 a, c, g dbSNP:147203739
77 77 a, g dbSNP:772125233
79 79 c, t dbSNP:201792944
81 81 a, g dbSNP:768830118
82 82 c, t dbSNP:140505486
89 89 a, g dbSNP:769936524
97 97 a, g dbSNP:775729620
103 103 a, g dbSNP:763137483
121 121 c, t dbSNP:377732811
122 122 a, g dbSNP:777722463
123 123 c, t dbSNP:746797640
124 124 c, t dbSNP:770806014
131 131 a, g, t dbSNP:776256103
161 161 a, g dbSNP:753624585
163 163 a, c, g dbSNP:141206174
177 177 a, g dbSNP:765008447
180 180 a, g dbSNP:752450196
187 187 c, g dbSNP:762485960
189 189 a, g dbSNP:374454766
190 190 c, t dbSNP:763729441
193 193 a, g dbSNP:751022322
216 216 c, g dbSNP:757233633
224 224 c, g dbSNP:781041301
226 226 c, g dbSNP:150734586
230 230 a, c dbSNP:755951683
238 238 c, t dbSNP:779793507
247 247 c, g, t dbSNP:748434313
248 248 a, t dbSNP:777919893
250 250 c, t dbSNP:573011438
255 255 a, g dbSNP:771496966
259 259 a, g dbSNP:139048680
260 260 a, g dbSNP:368705995
268 268 c, t dbSNP:770326364
271 271 a, g dbSNP:775996281
278 278 c, t dbSNP:762726444
281 281 a, g dbSNP:556275941
296 296 a, c dbSNP:778130614
299 299 a, g dbSNP:768951742
302 302 a, g dbSNP:182195687
304 304 a, c dbSNP:747812796
318 318 a, g dbSNP:377199824
325 325 c, t dbSNP:772711384
327 327 c, t dbSNP:760136386
330 330 a, c dbSNP:766322894
331 331 a, g dbSNP:776479146
332 332 c, g dbSNP:759489827
334 334 g, t dbSNP:765011656
351 351 -, atg dbSNP:769502605
357 357 a, c dbSNP:752526721
359 359 a, g dbSNP:757568499
362 362 a, g dbSNP:370033991
370 370 a, g, t dbSNP:750563135
373 373 -, cac dbSNP:775127785
373 373 a, c dbSNP:780159761
376 376 c, t dbSNP:749826758
383 383 c, g dbSNP:755496976
388 388 c, t dbSNP:779156750
389 389 g, t dbSNP:748442517
402 402 a, g dbSNP:771741224
409 409 a, g dbSNP:373180201
415 415 c, t dbSNP:746637617
417 417 c, g dbSNP:770384643
427 427 c, t dbSNP:776204033
430 430 c, t dbSNP:759453504
438 438 a, g dbSNP:559661693
442 442 c, g dbSNP:775155431
446 446 c, g dbSNP:762797076
450 450 a, c dbSNP:200282253
458 458 a, g dbSNP:750724221
462 462 c, t dbSNP:760949110
467 467 a, g dbSNP:149885670
470 470 a, c dbSNP:753897217
474 474 c, g, t dbSNP:765207539
475 475 a, c, g dbSNP:758898123
487 487 c, g dbSNP:200637771
493 493 c, t dbSNP:146418156
507 507 a, g dbSNP:113613489
508 508 a, g dbSNP:756866342
514 514 a, g dbSNP:373225799
524 524 a, g dbSNP:745394027
525 525 c, g dbSNP:769396929
538 538 a, g dbSNP:17850721
553 553 c, t dbSNP:779679844
556 556 a, g dbSNP:543352858
581 581 c, g dbSNP:768655922
592 592 c, t dbSNP:774293070
595 595 c, t dbSNP:376539678
614 614 a, g dbSNP:771079572
623 623 g, t dbSNP:776957366
627 627 a, g, t dbSNP:139780199
628 628 c, t dbSNP:200828715
635 635 a, g dbSNP:775530911
637 637 c, g dbSNP:763468580
646 646 c, t dbSNP:748181994
647 647 a, t dbSNP:144503053
658 658 a, g dbSNP:146607451
660 660 c, g dbSNP:746719599
667 667 c, t dbSNP:770038359
696 696 c, g dbSNP:780203550
700 700 c, t dbSNP:749491356
702 702 c, g dbSNP:768764962
706 706 a, c, g dbSNP:149180222
708 708 c, t dbSNP:772391489
709 709 g, t dbSNP:368583920
746 746 a, g dbSNP:373486719
748 748 c, t dbSNP:766826666
763 763 a, g dbSNP:180973800
765 765 c, t dbSNP:549498464
773 773 a, g dbSNP:113912958
798 798 a, g dbSNP:373676771
799 799 c, t dbSNP:752087000
809 809 c, g dbSNP:758364307
817 817 a, g dbSNP:777534174
822 822 a, g dbSNP:751396536
826 826 a, g dbSNP:187368856
830 830 a, g dbSNP:780934823
834 834 a, g dbSNP:749579216
843 843 g, t dbSNP:768854709
847 847 a, g dbSNP:779064587
849 849 a, c dbSNP:748136044
861 861 a, g dbSNP:561386917
871 871 c, t dbSNP:750161964
872 872 c, g dbSNP:755258124
876 876 a, g dbSNP:778951638
887 887 g, t dbSNP:748380323
916 916 c, t dbSNP:143296563
917 917 a, g dbSNP:777901369
920 920 c, t dbSNP:747572749
922 922 a, g dbSNP:771505562
931 931 c, t dbSNP:777136708
933 933 a, g dbSNP:201795443
941 941 a, g dbSNP:769511426
944 944 a, g dbSNP:775059137
951 951 a, t dbSNP:762640222
956 956 c, t dbSNP:763579465
984 984 a, g dbSNP:550279324
1014 1014 a, g dbSNP:773837230
1020 1020 c, g dbSNP:71329463
1021 1021 c, t dbSNP:761753616
1031 1031 c, g dbSNP:765406792
1038 1038 a, c dbSNP:151266236
1041 1041 c, g dbSNP:766375927
1054 1054 c, t dbSNP:757463604
1059 1059 a, g dbSNP:201549256
1064 1064 c, t dbSNP:750961802
1074 1074 a, c dbSNP:762126832
1076 1076 a, g dbSNP:568113525
1088 1088 c, g dbSNP:750461208
1095 1095 c, t dbSNP:756584765
1098 1098 a, g dbSNP:780572230
1110 1110 a, c dbSNP:755463134
1112 1112 g, t dbSNP:541906192
1114 1114 a, g dbSNP:752989102
1142 1142 g, t dbSNP:758074601
1145 1145 a, g dbSNP:777431811
1153 1153 a, g dbSNP:746617487
1165 1165 c, t dbSNP:370548139
1170 1170 a, g, t dbSNP:749141828
1171 1171 c, t dbSNP:745862670
1176 1176 a, c dbSNP:769800257
1182 1182 a, g dbSNP:775430263
1201 1201 a, t dbSNP:749052439
1204 1204 c, g dbSNP:772207979
1206 1206 a, g dbSNP:773521736
1207 1207 a, g dbSNP:760801544
1213 1213 g, t dbSNP:369419016
1215 1215 c, t dbSNP:766538628
1217 1217 a, g dbSNP:776515950
1226 1226 a, t dbSNP:759560086
1230 1230 a, g dbSNP:769840252
1234 1234 a, g dbSNP:189488022
1235 1235 a, g dbSNP:763368047
1241 1241 a, c dbSNP:764581545
1247 1247 a, g dbSNP:751816550
1256 1256 a, c dbSNP:749359827
1260 1260 a, g dbSNP:761535308
1283 1283 c, g dbSNP:767038055
1291 1291 c, t dbSNP:376649965
1292 1292 a, g dbSNP:755735886
1294 1294 c, t dbSNP:779704391
1303 1303 a, g dbSNP:753883803
1305 1305 c, t dbSNP:754812668
1306 1306 c, t dbSNP:778774026
1316 1316 c, g dbSNP:747853630
1322 1322 a, g dbSNP:200360700
1324 1324 c, t dbSNP:571122134
1325 1325 a, g dbSNP:779579241
1334 1334 g, t dbSNP:781439045
1336 1336 c, t dbSNP:748602761
1337 1337 a, g dbSNP:538447999
1354 1354 g, t dbSNP:776226649
1355 1355 a, c dbSNP:759017252
1363 1363 c, t dbSNP:764772705
1371 1371 c, g dbSNP:752642647
1376 1376 c, g dbSNP:758320166
1377 1377 -, t dbSNP:761072260
1384 1384 a, c, t dbSNP:763891431
1395 1395 a, g dbSNP:200670756
1401 1401 a, c dbSNP:780205458
1402 1402 g, t dbSNP:749508131
1409 1409 a, g dbSNP:201802087
1418 1418 a, g dbSNP:779048302
1419 1419 c, t dbSNP:775029900
1421 1421 a, c dbSNP:772636858
1430 1430 c, g dbSNP:773739451
1431 1431 c, t dbSNP:747325080
1432 1432 a, g dbSNP:771399567
1435 1435 a, c dbSNP:776186420
1439 1439 c, t dbSNP:759221648
1440 1440 a, g dbSNP:764710364
1451 1451 a, g dbSNP:41311143
1454 1454 a, g dbSNP:762883675
1455 1455 -, gcc dbSNP:766881855
1469 1469 c, t dbSNP:138287627
1470 1470 a, g dbSNP:751377720
1477 1477 -, ccaggaggc dbSNP:779863218
1478 1478 -, ccaggaggc dbSNP:755863352
1486 1486 c, t dbSNP:568182920
1495 1495 c, t dbSNP:752168245
1498 1498 g, t dbSNP:757722744
1505 1505 c, t dbSNP:781417091
1507 1507 c, g dbSNP:746205642
1508 1508 a, g dbSNP:143781060
1510 1510 a, g dbSNP:779806745
1511 1511 a, g dbSNP:371203156
1515 1515 a, g dbSNP:768226495
1521 1521 c, g dbSNP:773841106
1525 1525 c, g dbSNP:761755379
1526 1526 c, t dbSNP:772003903
1527 1527 a, g dbSNP:146069416
1529 1529 -, gga dbSNP:748914699
1529 1529 a, g dbSNP:760562435
1546 1546 c, t dbSNP:766057311
1561 1561 a, g dbSNP:753034592
1564 1564 c, t dbSNP:763253549
1566 1566 a, g dbSNP:764280754
1575 1575 a, g, t dbSNP:751624817
1576 1576 a, c dbSNP:781689077
1578 1578 a, g dbSNP:750873132
1583 1583 a, c, t dbSNP:756545471
1588 1588 c, g, t dbSNP:749000594
1591 1591 a, g dbSNP:778423646
1593 1593 c, g dbSNP:747642485
1594 1594 a, t dbSNP:375087308
1601 1601 a, g dbSNP:535564625
1608 1608 a, c dbSNP:746916052
1627 1627 a, g dbSNP:770816500
1633 1633 c, g dbSNP:776510331
1638 1638 c, t dbSNP:371101669
1656 1656 a, g dbSNP:201950459
1660 1660 c, t dbSNP:138837921
1672 1672 a, g dbSNP:374886351
1696 1696 a, g dbSNP:754318835
1707 1707 c, g dbSNP:755371218
1711 1711 c, t dbSNP:765577876
1716 1716 c, t dbSNP:149288880
1717 1717 a, g dbSNP:144536392
1726 1726 c, t dbSNP:369377914
1728 1728 c, t dbSNP:372053944
1729 1729 a, g dbSNP:757284121
1732 1732 c, t dbSNP:373600548
1734 1734 c, t dbSNP:745768944
1745 1745 c, g, t dbSNP:199957418
1746 1746 a, g dbSNP:747223579
1764 1764 c, g dbSNP:771052631
1773 1773 a, g dbSNP:776633985
1775 1775 g, t dbSNP:760040574
1783 1783 a, g dbSNP:770158019
1799 1799 c, t dbSNP:375159283
1800 1800 a, g dbSNP:763277358
1810 1810 c, t dbSNP:148438243
1811 1811 a, g dbSNP:751324003
1817 1817 a, g dbSNP:761544613
1822 1822 a, c, g dbSNP:368221410
1839 1839 g, t dbSNP:756154356
1849 1849 g, t dbSNP:766181979
1853 1853 c, t dbSNP:753775393
1856 1856 c, t dbSNP:754794055
1862 1862 a, g dbSNP:371220999
1864 1864 c, t dbSNP:575816889
1866 1866 a, g dbSNP:757403640
1869 1869 a, g dbSNP:368700556
1880 1880 a, g dbSNP:745897992
1882 1882 g, t dbSNP:769896639
1885 1885 c, t dbSNP:182236482
1894 1894 a, t dbSNP:564325903
1902 1902 a, g dbSNP:201365388
1903 1903 a, c dbSNP:749797583
1909 1909 c, g, t dbSNP:111270799
1910 1910 c, t dbSNP:774802216
1912 1912 a, t dbSNP:373731597
1920 1920 c, t dbSNP:767241430
1937 1937 g, t dbSNP:202103106
1941 1941 a, c dbSNP:200316952
1942 1942 a, g dbSNP:765905902
1943 1943 a, t dbSNP:540524061
1950 1950 -, gaa dbSNP:779809862
1950 1950 c, g dbSNP:754883032
1957 1957 a, g dbSNP:764945495
1965 1965 a, g dbSNP:752449468
1991 1991 a, g dbSNP:145953477
1993 1993 c, t dbSNP:769261609
2010 2010 a, g dbSNP:775473690
2013 2013 a, g dbSNP:762865145
2019 2019 a, g dbSNP:772073607
2020 2020 a, g, t dbSNP:763891504
2024 2024 -, actagatg dbSNP:747507384
2031 2031 a, t dbSNP:139786904
2033 2033 c, g dbSNP:760840445
2042 2042 c, t dbSNP:370708728
2043 2043 a, g dbSNP:200423555
2045 2045 a, g dbSNP:755153099
2048 2048 -, g dbSNP:777279508
2049 2049 c, t dbSNP:779118399
2054 2054 c, t dbSNP:368593909
2057 2057 a, g dbSNP:758905291
2059 2059 a, t dbSNP:778310719
2062 2062 a, c dbSNP:370466002
2079 2079 c, g dbSNP:747348720
2104 2104 a, t dbSNP:371953876
2125 2125 a, c dbSNP:3205130
2153 2153 c, t dbSNP:544683947
2158 2158 c, g dbSNP:728760
2171 2171 -, t dbSNP:532857025
2183 2183 c, g dbSNP:557978659
2193 2193 a, g dbSNP:758967536
2199 2199 a, g dbSNP:573094995
2209 2209 g, t dbSNP:540536395
2211 2211 a, g dbSNP:562200272
2224 2224 c, t dbSNP:11545666
2248 2248 -, taac dbSNP:551098529
2254 2254 c, t dbSNP:149241548
2257 2257 -, aaca dbSNP:370714754
2272 2272 c, t dbSNP:193121445
2316 2316 c, t dbSNP:1055852
2346 2346 -, g dbSNP:537969245
2347 2347 -, t dbSNP:776760592
2351 2351 g, t dbSNP:528944830
2360 2360 -, ttt dbSNP:76631619
2362 2362 a, t dbSNP:551900901
2384 2384 c, t dbSNP:375258481
2388 2388 c, g dbSNP:560429326

Target ORF information:

RefSeq Version XM_011529997
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529997

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CGCCACCCTT TCCGCCCCCG
GGTGGTGATC GTGGCAGAGG TGGCCCTGGT GGCATGCGGG GAGGAAGAGG TGGCCTCATG
GATCGTGGTG GTCCCGGTGG AATGTTCAGA GGTGGCCGTG GTGGAGACAG AGGTGGCTTC
CGTGGTGGCC GGGGCATGGA CCGAGGTGGC TTTGGTGGAG GAAGACGAGG TGGCCCTGGG
GGGCCCCCTG GACCTTTGAT GGAACAGATG GGAGGAAGAA GAGGAGGACG TGGAGGACCT
GGAAAAATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ATCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57615
Accession Version XM_011529998.1
Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAGTGC
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCGC CACCCTTTCC GCCCCCGGGT
GGTGATCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAT
CGTGGTGGTC CCGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT
GGTGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGTGG CCCTGGGGGG
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GAGGACGTGG AGGACCTGGA
AAAATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGATC GGCCCTACTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528300.1
CDS56..2026
Misc Feature(1)1136..1387(+)
Misc Feature(2)1142..1381(+)
Misc Feature(3)1607..1696(+)
Misc Feature(4)1619..1684(+)
Misc Feature(5)1625..1684(+)
Translation
Position Chain Variation Link
3 3 a, c dbSNP:551097984
5 5 c, g dbSNP:770561780
7 7 a, c, g dbSNP:758447329
8 8 a, c dbSNP:758975710
11 11 a, c, t dbSNP:111892587
14 14 a, g dbSNP:367933864
19 19 a, c dbSNP:763833724
21 21 a, g dbSNP:751395202
22 22 a, c, g dbSNP:756462778
23 23 a, g dbSNP:754003425
25 25 a, g dbSNP:754966628
27 27 -, g dbSNP:760330065
31 31 a, g dbSNP:199589576
35 35 a, t dbSNP:748647019
36 36 a, g dbSNP:758943413
37 37 c, g dbSNP:201886504
38 38 c, g, t dbSNP:377068270
39 39 a, g dbSNP:776222972
41 41 c, g dbSNP:745403052
47 47 a, g dbSNP:375095904
48 48 a, g dbSNP:552992152
52 52 c, g dbSNP:762864394
53 53 a, g dbSNP:2231392
62 62 a, t dbSNP:774293267
64 64 c, t dbSNP:779516758
65 65 a, c dbSNP:761661070
67 67 a, c, g dbSNP:147203739
77 77 a, g dbSNP:772125233
79 79 c, t dbSNP:201792944
81 81 a, g dbSNP:768830118
82 82 c, t dbSNP:140505486
89 89 a, g dbSNP:769936524
97 97 a, g dbSNP:775729620
103 103 a, g dbSNP:763137483
121 121 c, t dbSNP:377732811
122 122 a, g dbSNP:777722463
123 123 c, t dbSNP:746797640
124 124 c, t dbSNP:770806014
131 131 a, g, t dbSNP:776256103
161 161 a, g dbSNP:753624585
163 163 a, c, g dbSNP:141206174
177 177 a, g dbSNP:765008447
180 180 a, g dbSNP:752450196
187 187 c, g dbSNP:762485960
189 189 a, g dbSNP:374454766
190 190 c, t dbSNP:763729441
193 193 a, g dbSNP:751022322
216 216 c, g dbSNP:757233633
224 224 c, g dbSNP:781041301
226 226 c, g dbSNP:150734586
230 230 a, c dbSNP:755951683
238 238 c, t dbSNP:779793507
247 247 c, g, t dbSNP:748434313
248 248 a, t dbSNP:777919893
250 250 c, t dbSNP:573011438
255 255 a, g dbSNP:771496966
259 259 a, g dbSNP:139048680
260 260 a, g dbSNP:368705995
268 268 c, t dbSNP:770326364
271 271 a, g dbSNP:775996281
278 278 c, t dbSNP:762726444
281 281 a, g dbSNP:556275941
296 296 a, c dbSNP:778130614
299 299 a, g dbSNP:768951742
302 302 a, g dbSNP:182195687
304 304 a, c dbSNP:747812796
318 318 a, g dbSNP:377199824
325 325 c, t dbSNP:772711384
327 327 c, t dbSNP:760136386
330 330 a, c dbSNP:766322894
331 331 a, g dbSNP:776479146
332 332 c, g dbSNP:759489827
334 334 g, t dbSNP:765011656
351 351 -, atg dbSNP:769502605
357 357 a, c dbSNP:752526721
359 359 a, g dbSNP:757568499
362 362 a, g dbSNP:370033991
370 370 a, g, t dbSNP:750563135
373 373 -, cac dbSNP:775127785
373 373 a, c dbSNP:780159761
376 376 c, t dbSNP:749826758
383 383 c, g dbSNP:755496976
388 388 c, t dbSNP:779156750
389 389 g, t dbSNP:748442517
402 402 a, g dbSNP:771741224
409 409 a, g dbSNP:373180201
415 415 c, t dbSNP:746637617
417 417 c, g dbSNP:770384643
427 427 c, t dbSNP:776204033
430 430 c, t dbSNP:759453504
438 438 a, g dbSNP:559661693
442 442 c, g dbSNP:775155431
446 446 c, g dbSNP:762797076
450 450 a, c dbSNP:200282253
458 458 a, g dbSNP:750724221
462 462 c, t dbSNP:760949110
467 467 a, g dbSNP:149885670
470 470 a, c dbSNP:753897217
474 474 c, g, t dbSNP:765207539
475 475 a, c, g dbSNP:758898123
487 487 c, g dbSNP:200637771
493 493 c, t dbSNP:146418156
507 507 a, g dbSNP:113613489
508 508 a, g dbSNP:756866342
514 514 a, g dbSNP:373225799
524 524 a, g dbSNP:745394027
525 525 c, g dbSNP:769396929
538 538 a, g dbSNP:17850721
553 553 c, t dbSNP:779679844
556 556 a, g dbSNP:543352858
581 581 c, g dbSNP:768655922
592 592 c, t dbSNP:774293070
595 595 c, t dbSNP:376539678
614 614 a, g dbSNP:771079572
623 623 g, t dbSNP:776957366
627 627 a, g, t dbSNP:139780199
628 628 c, t dbSNP:200828715
635 635 a, g dbSNP:775530911
637 637 c, g dbSNP:763468580
646 646 c, t dbSNP:748181994
647 647 a, t dbSNP:144503053
658 658 a, g dbSNP:146607451
660 660 c, g dbSNP:746719599
667 667 c, t dbSNP:770038359
696 696 c, g dbSNP:780203550
700 700 c, t dbSNP:749491356
702 702 c, g dbSNP:768764962
706 706 a, c, g dbSNP:149180222
708 708 c, t dbSNP:772391489
709 709 g, t dbSNP:368583920
746 746 a, g dbSNP:373486719
748 748 c, t dbSNP:766826666
763 763 a, g dbSNP:180973800
765 765 c, t dbSNP:549498464
773 773 a, g dbSNP:113912958
798 798 a, g dbSNP:373676771
799 799 c, t dbSNP:752087000
809 809 c, g dbSNP:758364307
817 817 a, g dbSNP:777534174
822 822 a, g dbSNP:751396536
826 826 a, g dbSNP:187368856
830 830 a, g dbSNP:780934823
834 834 a, g dbSNP:749579216
843 843 g, t dbSNP:768854709
847 847 a, g dbSNP:779064587
849 849 a, c dbSNP:748136044
861 861 a, g dbSNP:561386917
871 871 c, t dbSNP:750161964
872 872 c, g dbSNP:755258124
876 876 a, g dbSNP:778951638
887 887 g, t dbSNP:748380323
916 916 c, t dbSNP:143296563
917 917 a, g dbSNP:777901369
920 920 c, t dbSNP:747572749
922 922 a, g dbSNP:771505562
931 931 c, t dbSNP:777136708
933 933 a, g dbSNP:201795443
941 941 a, g dbSNP:769511426
944 944 a, g dbSNP:775059137
951 951 a, t dbSNP:762640222
956 956 c, t dbSNP:763579465
984 984 a, g dbSNP:550279324
1014 1014 a, g dbSNP:773837230
1020 1020 c, g dbSNP:71329463
1021 1021 c, t dbSNP:761753616
1031 1031 c, g dbSNP:765406792
1035 1035 a, c dbSNP:151266236
1038 1038 c, g dbSNP:766375927
1051 1051 c, t dbSNP:757463604
1056 1056 a, g dbSNP:201549256
1061 1061 c, t dbSNP:750961802
1071 1071 a, c dbSNP:762126832
1073 1073 a, g dbSNP:568113525
1085 1085 c, g dbSNP:750461208
1092 1092 c, t dbSNP:756584765
1095 1095 a, g dbSNP:780572230
1107 1107 a, c dbSNP:755463134
1109 1109 g, t dbSNP:541906192
1111 1111 a, g dbSNP:752989102
1139 1139 g, t dbSNP:758074601
1142 1142 a, g dbSNP:777431811
1150 1150 a, g dbSNP:746617487
1162 1162 c, t dbSNP:370548139
1167 1167 a, g, t dbSNP:749141828
1168 1168 c, t dbSNP:745862670
1173 1173 a, c dbSNP:769800257
1179 1179 a, g dbSNP:775430263
1198 1198 a, t dbSNP:749052439
1201 1201 c, g dbSNP:772207979
1203 1203 a, g dbSNP:773521736
1204 1204 a, g dbSNP:760801544
1210 1210 g, t dbSNP:369419016
1212 1212 c, t dbSNP:766538628
1214 1214 a, g dbSNP:776515950
1223 1223 a, t dbSNP:759560086
1227 1227 a, g dbSNP:769840252
1231 1231 a, g dbSNP:189488022
1232 1232 a, g dbSNP:763368047
1238 1238 a, c dbSNP:764581545
1244 1244 a, g dbSNP:751816550
1253 1253 a, c dbSNP:749359827
1257 1257 a, g dbSNP:761535308
1280 1280 c, g dbSNP:767038055
1288 1288 c, t dbSNP:376649965
1289 1289 a, g dbSNP:755735886
1291 1291 c, t dbSNP:779704391
1300 1300 a, g dbSNP:753883803
1302 1302 c, t dbSNP:754812668
1303 1303 c, t dbSNP:778774026
1313 1313 c, g dbSNP:747853630
1319 1319 a, g dbSNP:200360700
1321 1321 c, t dbSNP:571122134
1322 1322 a, g dbSNP:779579241
1331 1331 g, t dbSNP:781439045
1333 1333 c, t dbSNP:748602761
1334 1334 a, g dbSNP:538447999
1351 1351 g, t dbSNP:776226649
1352 1352 a, c dbSNP:759017252
1360 1360 c, t dbSNP:764772705
1368 1368 c, g dbSNP:752642647
1373 1373 c, g dbSNP:758320166
1374 1374 -, t dbSNP:761072260
1381 1381 a, c, t dbSNP:763891431
1392 1392 a, g dbSNP:200670756
1398 1398 a, c dbSNP:780205458
1399 1399 g, t dbSNP:749508131
1406 1406 a, g dbSNP:201802087
1415 1415 a, g dbSNP:779048302
1416 1416 c, t dbSNP:775029900
1418 1418 a, c dbSNP:772636858
1427 1427 c, g dbSNP:773739451
1428 1428 c, t dbSNP:747325080
1429 1429 a, g dbSNP:771399567
1432 1432 a, c dbSNP:776186420
1436 1436 c, t dbSNP:759221648
1437 1437 a, g dbSNP:764710364
1448 1448 a, g dbSNP:41311143
1451 1451 a, g dbSNP:762883675
1452 1452 -, gcc dbSNP:766881855
1466 1466 c, t dbSNP:138287627
1467 1467 a, g dbSNP:751377720
1474 1474 -, ccaggaggc dbSNP:779863218
1475 1475 -, ccaggaggc dbSNP:755863352
1483 1483 c, t dbSNP:568182920
1492 1492 c, t dbSNP:752168245
1495 1495 g, t dbSNP:757722744
1502 1502 c, t dbSNP:781417091
1504 1504 c, g dbSNP:746205642
1505 1505 a, g dbSNP:143781060
1507 1507 a, g dbSNP:779806745
1508 1508 a, g dbSNP:371203156
1512 1512 a, g dbSNP:768226495
1518 1518 c, g dbSNP:773841106
1522 1522 c, g dbSNP:761755379
1523 1523 c, t dbSNP:772003903
1524 1524 a, g dbSNP:146069416
1526 1526 -, gga dbSNP:748914699
1526 1526 a, g dbSNP:760562435
1543 1543 c, t dbSNP:766057311
1558 1558 a, g dbSNP:753034592
1561 1561 c, t dbSNP:763253549
1563 1563 a, g dbSNP:764280754
1572 1572 a, g, t dbSNP:751624817
1573 1573 a, c dbSNP:781689077
1575 1575 a, g dbSNP:750873132
1580 1580 a, c, t dbSNP:756545471
1585 1585 c, g, t dbSNP:749000594
1588 1588 a, g dbSNP:778423646
1590 1590 c, g dbSNP:747642485
1591 1591 a, t dbSNP:375087308
1598 1598 a, g dbSNP:535564625
1605 1605 a, c dbSNP:746916052
1624 1624 a, g dbSNP:770816500
1630 1630 c, g dbSNP:776510331
1635 1635 c, t dbSNP:371101669
1653 1653 a, g dbSNP:201950459
1657 1657 c, t dbSNP:138837921
1669 1669 a, g dbSNP:374886351
1693 1693 a, g dbSNP:754318835
1704 1704 c, g dbSNP:755371218
1708 1708 c, t dbSNP:765577876
1713 1713 c, t dbSNP:149288880
1714 1714 a, g dbSNP:144536392
1723 1723 c, t dbSNP:369377914
1725 1725 c, t dbSNP:372053944
1726 1726 a, g dbSNP:757284121
1729 1729 c, t dbSNP:373600548
1731 1731 c, t dbSNP:745768944
1742 1742 c, g, t dbSNP:199957418
1743 1743 a, g dbSNP:747223579
1761 1761 c, g dbSNP:771052631
1770 1770 a, g dbSNP:776633985
1772 1772 g, t dbSNP:760040574
1780 1780 a, g dbSNP:770158019
1796 1796 c, t dbSNP:375159283
1797 1797 a, g dbSNP:763277358
1807 1807 c, t dbSNP:148438243
1808 1808 a, g dbSNP:751324003
1814 1814 a, g dbSNP:761544613
1819 1819 a, c, g dbSNP:368221410
1836 1836 g, t dbSNP:756154356
1846 1846 g, t dbSNP:766181979
1850 1850 c, t dbSNP:753775393
1853 1853 c, t dbSNP:754794055
1859 1859 a, g dbSNP:371220999
1861 1861 c, t dbSNP:575816889
1863 1863 a, g dbSNP:757403640
1866 1866 a, g dbSNP:368700556
1877 1877 a, g dbSNP:745897992
1879 1879 g, t dbSNP:769896639
1882 1882 c, t dbSNP:182236482
1891 1891 a, t dbSNP:564325903
1899 1899 a, g dbSNP:201365388
1900 1900 a, c dbSNP:749797583
1906 1906 c, g, t dbSNP:111270799
1907 1907 c, t dbSNP:774802216
1909 1909 a, t dbSNP:373731597
1917 1917 c, t dbSNP:767241430
1934 1934 g, t dbSNP:202103106
1938 1938 a, c dbSNP:200316952
1939 1939 a, g dbSNP:765905902
1940 1940 a, t dbSNP:540524061
1947 1947 -, gaa dbSNP:779809862
1947 1947 c, g dbSNP:754883032
1954 1954 a, g dbSNP:764945495
1962 1962 a, g dbSNP:752449468
1988 1988 a, g dbSNP:145953477
1990 1990 c, t dbSNP:769261609
2007 2007 a, g dbSNP:775473690
2010 2010 a, g dbSNP:762865145
2016 2016 a, g dbSNP:772073607
2017 2017 a, g, t dbSNP:763891504
2021 2021 -, actagatg dbSNP:747507384
2028 2028 a, t dbSNP:139786904
2030 2030 c, g dbSNP:760840445
2039 2039 c, t dbSNP:370708728
2040 2040 a, g dbSNP:200423555
2042 2042 a, g dbSNP:755153099
2045 2045 -, g dbSNP:777279508
2046 2046 c, t dbSNP:779118399
2051 2051 c, t dbSNP:368593909
2054 2054 a, g dbSNP:758905291
2056 2056 a, t dbSNP:778310719
2059 2059 a, c dbSNP:370466002
2076 2076 c, g dbSNP:747348720
2101 2101 a, t dbSNP:371953876
2122 2122 a, c dbSNP:3205130
2150 2150 c, t dbSNP:544683947
2155 2155 c, g dbSNP:728760
2168 2168 -, t dbSNP:532857025
2180 2180 c, g dbSNP:557978659
2190 2190 a, g dbSNP:758967536
2196 2196 a, g dbSNP:573094995
2206 2206 g, t dbSNP:540536395
2208 2208 a, g dbSNP:562200272
2221 2221 c, t dbSNP:11545666
2245 2245 -, taac dbSNP:551098529
2251 2251 c, t dbSNP:149241548
2254 2254 -, aaca dbSNP:370714754
2269 2269 c, t dbSNP:193121445
2313 2313 c, t dbSNP:1055852
2343 2343 -, g dbSNP:537969245
2344 2344 -, t dbSNP:776760592
2348 2348 g, t dbSNP:528944830
2357 2357 -, ttt dbSNP:76631619
2359 2359 a, t dbSNP:551900901
2381 2381 c, t dbSNP:375258481
2385 2385 c, g dbSNP:560429326

Target ORF information:

RefSeq Version XM_011529998
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529998

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAGTGC
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCGC CACCCTTTCC GCCCCCGGGT
GGTGATCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAT
CGTGGTGGTC CCGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT
GGTGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGTGG CCCTGGGGGG
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GAGGACGTGG AGGACCTGGA
AAAATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGATC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57616
Accession Version XM_011529999.1
Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TGGTGATCGT
GGCAGAGGTG GCCCTGGTGG CATGCGGGGA GGAAGAGGTG GCCTCATGGA TCGTGGTGGT
CCCGGTGGAA TGTTCAGAGG TGGCCGTGGT GGAGACAGAG GTGGCTTCCG TGGTGGCCGG
GGCATGGACC GAGGTGGCTT TGGTGGAGGA AGACGAGGTG GCCCTGGGGG GCCCCCTGGA
CCTTTGATGG AACAGATGGG AGGAAGAAGA GGAGGACGTG GAGGACCTGG AAAAATGGAT
AAAGGCGAGC ACCGTCAGGA GCGCAGAGAT CGGCCCTACT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011528301.1
CDS56..2017
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..>1726(+)
Misc Feature(4)1622..1726(+)
Misc Feature(5)1628..1726(+)
Translation
Position Chain Variation Link
3 3 a, c dbSNP:551097984
5 5 c, g dbSNP:770561780
7 7 a, c, g dbSNP:758447329
8 8 a, c dbSNP:758975710
11 11 a, c, t dbSNP:111892587
14 14 a, g dbSNP:367933864
19 19 a, c dbSNP:763833724
21 21 a, g dbSNP:751395202
22 22 a, c, g dbSNP:756462778
23 23 a, g dbSNP:754003425
25 25 a, g dbSNP:754966628
27 27 -, g dbSNP:760330065
31 31 a, g dbSNP:199589576
35 35 a, t dbSNP:748647019
36 36 a, g dbSNP:758943413
37 37 c, g dbSNP:201886504
38 38 c, g, t dbSNP:377068270
39 39 a, g dbSNP:776222972
41 41 c, g dbSNP:745403052
47 47 a, g dbSNP:375095904
48 48 a, g dbSNP:552992152
52 52 c, g dbSNP:762864394
53 53 a, g dbSNP:2231392
62 62 a, t dbSNP:774293267
64 64 c, t