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F2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol F2
Entrez Gene ID 2147
Full Name coagulation factor II (thrombin)
Synonyms PT, RPRGL2, THPH1
General protein information
Preferred Names
prothrombin
Names
prothrombin
serine protease
prothrombin B-chain
prepro-coagulation factor II
NP_000497.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p11

Summary Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

176930

Disorder Html: Hypoprothrombinemia, 613679 (3); Dysprothrombinemia, 613679 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_000506 NP_000497 prothrombin isoform 1 preproprotein
NM_001311257 NP_001298186 prothrombin isoform 2

KEGG
hsa04080 Neuroactive ligand-receptor interaction
hsa04810 Regulation of actin cytoskeleton
hsa04610 Complement and coagulation cascades
Reactome
R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-109582 Hemostasis
R-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-HSA-162582 Signal Transduction
R-HSA-140875 Common Pathway of Fibrin Clot Formation
R-HSA-140837 Intrinsic Pathway of Fibrin Clot Formation
R-HSA-76009 Platelet Aggregation (Plug Formation)
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-140877 Formation of Fibrin Clot (Clotting Cascade)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signaling
R-HSA-416476 G alpha (q) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK
R-HSA-392499 Metabolism of proteins
R-HSA-159763 Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus
R-HSA-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation
R-HSA-159740 Gamma-carboxylation of protein precursors
R-HSA-159782 Removal of aminoterminal propeptides from gamma-carboxylated proteins
R-HSA-597592 Post-translational protein modification
R-HSA-159854 Gamma-carboxylation, transport, and amino-terminal cleavage of proteins
Pathway Interaction Database
angiopoietinreceptor_pathway Angiopoietin receptor Tie2-mediated signaling
hnf3bpathway FOXA2 and FOXA3 transcription factor networks
syndecan_4_pathway Syndecan-4-mediated signaling events
WikiPathways
WP15 Selenium Pathway
WP272 Blood Clotting Cascade
WP51 Regulation of Actin Cytoskeleton


Homo sapiens (human) F2 NP_000497.1
Pan troglodytes (chimpanzee) F2 XP_001165233.1
Macaca mulatta (Rhesus monkey) F2 NP_001098055.1
Canis lupus familiaris (dog) F2 XP_003639742.1
Bos taurus (cattle) F2 NP_776302.1
Mus musculus (house mouse) F2 NP_034298.1
Rattus norvegicus (Norway rat) F2 NP_075213.2
Gallus gallus (chicken) F2 NP_989936.1
Danio rerio (zebrafish) f2 NP_998555.1
Xenopus (Silurana) tropicalis (western clawed frog) f2 NP_001015797.1

Component

ID Name Evidence
GO:0005576 extracellular region EXP
GO:0005576 extracellular region TAS
GO:0005615 extracellular space IDA
GO:0005625 soluble fraction TAS
GO:0005788 endoplasmic reticulum lumen TAS
GO:0005796 Golgi lumen TAS
GO:0005886 plasma membrane EXP
GO:0005886 plasma membrane TAS

Function

ID Name Evidence
GO:0004252 serine-type endopeptidase activity EXP
GO:0004252 serine-type endopeptidase activity IDA
GO:0004252 serine-type endopeptidase activity TAS
GO:0005102 receptor binding IPI
GO:0005102 receptor binding IPI
GO:0005509 calcium ion binding IEA
GO:0005515 protein binding IPI
GO:0008083 growth factor activity TAS
GO:0008233 peptidase activity IEA
GO:0070053 thrombospondin receptor activity IDA

Process

ID Name Evidence
GO:0001934 positive regulation of protein phosphorylation IDA
GO:0006508 proteolysis TAS
GO:0006915 apoptosis TAS
GO:0006919 activation of caspase activity TAS
GO:0006953 acute-phase response IEA
GO:0007166 cell surface receptor linked signaling pathway IDA
GO:0007260 tyrosine phosphorylation of STAT protein TAS
GO:0007262 STAT protein import into nucleus TAS
GO:0007275 multicellular organismal development TAS
GO:0007596 blood coagulation TAS
GO:0007597 blood coagulation, intrinsic pathway TAS
GO:0008284 positive regulation of cell proliferation IEA
GO:0008360 regulation of cell shape IEA
GO:0009611 response to wounding IDA
GO:0010544 negative regulation of platelet activation TAS
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade IEA
GO:0017187 peptidyl-glutamic acid carboxylation TAS
GO:0030168 platelet activation IDA
GO:0030168 platelet activation TAS
GO:0030193 regulation of blood coagulation TAS
GO:0030194 positive regulation of blood coagulation IDA
GO:0030307 positive regulation of cell growth IEA
GO:0032967 positive regulation of collagen biosynthetic process IDA
GO:0042730 fibrinolysis IDA
GO:0043687 post-translational protein modification TAS
GO:0044267 cellular protein metabolic process TAS
GO:0045861 negative regulation of proteolysis IDA
GO:0048712 negative regulation of astrocyte differentiation IDA
GO:0050900 leukocyte migration TAS
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol IDA
GO:0051480 cytosolic calcium ion homeostasis IDA
GO:0051918 negative regulation of fibrinolysis TAS
GO:2000379 positive regulation of reactive oxygen species metabolic process IDA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26797 NM_000506 Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu57660 NM_001311257 Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu26797
Accession Version NM_000506.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-AUG-2015
Organism Homo sapiens (human)
Product prothrombin isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA638868.1, AK312965.1, AK303747.1 and BC051332.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2015 this sequence version replaced gi:169808403. Summary: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 20421939 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTGTAGC 
CTTGTGCACA GCCAGCATGT GTTCCTGGCT CCTCAGCAAG CACGGTCGCT GCTCCAGCGG
GTCCGGCGAG CCAACACCTT CTTGGAGGAG GTGCGCAAGG GCAACCTGGA GCGAGAGTGC
GTGGAGGAGA CGTGCAGCTA CGAGGAGGCC TTCGAGGCTC TGGAGTCCTC CACGGCTACG
GATGTGTTCT GGGCCAAGTA CACAGCTTGT GAGACAGCGA GGACGCCTCG AGATAAGCTT
GCTGCATGTC TGGAAGGTAA CTGTGCTGAG GGTCTGGGTA CGAACTACCG AGGGCATGTG
AACATCACCC GGTCAGGCAT TGAGTGCCAG CTATGGAGGA GTCGCTACCC ACATAAGCCT
GAAATCAACT CCACTACCCA TCCTGGGGCC GACCTACAGG AGAATTTCTG CCGCAACCCC
GACAGCAGCA CCACGGGACC CTGGTGCTAC ACTACAGACC CCACCGTGAG GAGGCAGGAA
TGCAGCATCC CTGTCTGTGG CCAGGATCAA GTCACTGTAG CGATGACTCC ACGCTCCGAA
GGCTCCAGTG TGAATCTGTC ACCTCCATTG GAGCAGTGTG TCCCTGATCG GGGGCAGCAG
TACCAGGGGC GCCTGGCGGT GACCACACAT GGGCTCCCCT GCCTGGCCTG GGCCAGCGCA
CAGGCCAAGG CCCTGAGCAA GCACCAGGAC TTCAACTCAG CTGTGCAGCT GGTGGAGAAC
TTCTGCCGCA ACCCAGACGG GGATGAGGAG GGCGTGTGGT GCTATGTGGC CGGGAAGCCT
GGCGACTTTG GGTACTGCGA CCTCAACTAT TGTGAGGAGG CCGTGGAGGA GGAGACAGGA
GATGGGCTGG ATGAGGACTC AGACAGGGCC ATCGAAGGGC GTACCGCCAC CAGTGAGTAC
CAGACTTTCT TCAATCCGAG GACCTTTGGC TCGGGAGAGG CAGACTGTGG GCTGCGACCT
CTGTTCGAGA AGAAGTCGCT GGAGGACAAA ACCGAAAGAG AGCTCCTGGA ATCCTACATC
GACGGGCGCA TTGTGGAGGG CTCGGATGCA GAGATCGGCA TGTCACCTTG GCAGGTGATG
CTTTTCCGGA AGAGTCCCCA GGAGCTGCTG TGTGGGGCCA GCCTCATCAG TGACCGCTGG
GTCCTCACCG CCGCCCACTG CCTCCTGTAC CCGCCCTGGG ACAAGAACTT CACCGAGAAT
GACCTTCTGG TGCGCATTGG CAAGCACTCC CGCACCAGGT ACGAGCGAAA CATTGAAAAG
ATATCCATGT TGGAAAAGAT CTACATCCAC CCCAGGTACA ACTGGCGGGA GAACCTGGAC
CGGGACATTG CCCTGATGAA GCTGAAGAAG CCTGTTGCCT TCAGTGACTA CATTCACCCT
GTGTGTCTGC CCGACAGGGA GACGGCAGCC AGCTTGCTCC AGGCTGGATA CAAGGGGCGG
GTGACAGGCT GGGGCAACCT GAAGGAGACG TGGACAGCCA ACGTTGGTAA GGGGCAGCCC
AGTGTCCTGC AGGTGGTGAA CCTGCCCATT GTGGAGCGGC CGGTCTGCAA GGACTCCACC
CGGATCCGCA TCACTGACAA CATGTTCTGT GCTGGTTACA AGCCTGATGA AGGGAAACGA
GGGGATGCCT GTGAAGGTGA CAGTGGGGGA CCCTTTGTCA TGAAGAGCCC CTTTAACAAC
CGCTGGTATC AAATGGGCAT CGTCTCATGG GGTGAAGGCT GTGACCGGGA TGGGAAATAT
GGCTTCTACA CACATGTGTT CCGCCTGAAG AAGTGGATAC AGAAGGTCAT TGATCAGTTT
GGAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000497.1
CDS71..1939
Misc Feature(1)62..64(+)
Misc Feature(2)143..334(+)
Misc Feature(3)215..217(+)
Misc Feature(4)218..220(+)
Misc Feature(5)239..241(+)
Misc Feature(6)245..247(+)
Misc Feature(7)254..256(+)
Misc Feature(8)257..259(+)
Misc Feature(9)272..274(+)
Misc Feature(10)275..277(+)
Misc Feature(11)284..286(+)
Misc Feature(12)293..295(+)
Misc Feature(13)383..628(+)
Misc Feature(14)419..421(+)
Misc Feature(15)431..433(+)
Misc Feature(16)464..604(+)
Misc Feature(17)497..499(+)
Misc Feature(18)662..664(+)
Misc Feature(19)701..946(+)
Misc Feature(20)734..736(+)
Misc Feature(21)779..919(+)
Misc Feature(22)1010..1012(+)
Misc Feature(23)1013..1159(+)
Misc Feature(24)1049..1051(+)
Misc Feature(25)1157..1909(+)
Misc Feature(26)1160..1918(+)
Misc Feature(27)1160..1162(+)
Misc Feature(28)1286..1774(+)
Misc Feature(29)1376..1378(+)
Misc Feature(30)1754..1843(+)
Exon (1)1..149
Gene:F2
Gene Synonym:
Exon (2)150..310
Gene:F2
Gene Synonym:
Exon (3)311..335
Gene:F2
Gene Synonym:
Exon (4)336..386
Gene:F2
Gene Synonym:
Exon (5)387..492
Gene:F2
Gene Synonym:
Exon (6)493..629
Gene:F2
Gene Synonym:
Exon (7)630..944
Gene:F2
Gene Synonym:
Exon (8)945..1073
Gene:F2
Gene Synonym:
Exon (9)1074..1200
Gene:F2
Gene Synonym:
Exon (10)1201..1368
Gene:F2
Gene Synonym:
Exon (11)1369..1542
Gene:F2
Gene Synonym:
Exon (12)1543..1724
Gene:F2
Gene Synonym:
Exon (13)1725..1795
Gene:F2
Gene Synonym:
Exon (14)1796..2039
Gene:F2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_000506
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000506

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTGTAGC 
CTTGTGCACA GCCAGCATGT GTTCCTGGCT CCTCAGCAAG CACGGTCGCT GCTCCAGCGG
GTCCGGCGAG CCAACACCTT CTTGGAGGAG GTGCGCAAGG GCAACCTGGA GCGAGAGTGC
GTGGAGGAGA CGTGCAGCTA CGAGGAGGCC TTCGAGGCTC TGGAGTCCTC CACGGCTACG
GATGTGTTCT GGGCCAAGTA CACAGCTTGT GAGACAGCGA GGACGCCTCG AGATAAGCTT
GCTGCATGTC TGGAAGGTAA CTGTGCTGAG GGTCTGGGTA CGAACTACCG AGGGCATGTG
AACATCACCC GGTCAGGCAT TGAGTGCCAG CTATGGAGGA GTCGCTACCC ACATAAGCCT
GAAATCAACT CCACTACCCA TCCTGGGGCC GACCTACAGG AGAATTTCTG CCGCAACCCC
GACAGCAGCA CCACGGGACC CTGGTGCTAC ACTACAGACC CCACCGTGAG GAGGCAGGAA
TGCAGCATCC CTGTCTGTGG CCAGGATCAA GTCACTGTAG CGATGACTCC ACGCTCCGAA
GGCTCCAGTG TGAATCTGTC ACCTCCATTG GAGCAGTGTG TCCCTGATCG GGGGCAGCAG
TACCAGGGGC GCCTGGCGGT GACCACACAT GGGCTCCCCT GCCTGGCCTG GGCCAGCGCA
CAGGCCAAGG CCCTGAGCAA GCACCAGGAC TTCAACTCAG CTGTGCAGCT GGTGGAGAAC
TTCTGCCGCA ACCCAGACGG GGATGAGGAG GGCGTGTGGT GCTATGTGGC CGGGAAGCCT
GGCGACTTTG GGTACTGCGA CCTCAACTAT TGTGAGGAGG CCGTGGAGGA GGAGACAGGA
GATGGGCTGG ATGAGGACTC AGACAGGGCC ATCGAAGGGC GTACCGCCAC CAGTGAGTAC
CAGACTTTCT TCAATCCGAG GACCTTTGGC TCGGGAGAGG CAGACTGTGG GCTGCGACCT
CTGTTCGAGA AGAAGTCGCT GGAGGACAAA ACCGAAAGAG AGCTCCTGGA ATCCTACATC
GACGGGCGCA TTGTGGAGGG CTCGGATGCA GAGATCGGCA TGTCACCTTG GCAGGTGATG
CTTTTCCGGA AGAGTCCCCA GGAGCTGCTG TGTGGGGCCA GCCTCATCAG TGACCGCTGG
GTCCTCACCG CCGCCCACTG CCTCCTGTAC CCGCCCTGGG ACAAGAACTT CACCGAGAAT
GACCTTCTGG TGCGCATTGG CAAGCACTCC CGCACCAGGT ACGAGCGAAA CATTGAAAAG
ATATCCATGT TGGAAAAGAT CTACATCCAC CCCAGGTACA ACTGGCGGGA GAACCTGGAC
CGGGACATTG CCCTGATGAA GCTGAAGAAG CCTGTTGCCT TCAGTGACTA CATTCACCCT
GTGTGTCTGC CCGACAGGGA GACGGCAGCC AGCTTGCTCC AGGCTGGATA CAAGGGGCGG
GTGACAGGCT GGGGCAACCT GAAGGAGACG TGGACAGCCA ACGTTGGTAA GGGGCAGCCC
AGTGTCCTGC AGGTGGTGAA CCTGCCCATT GTGGAGCGGC CGGTCTGCAA GGACTCCACC
CGGATCCGCA TCACTGACAA CATGTTCTGT GCTGGTTACA AGCCTGATGA AGGGAAACGA
GGGGATGCCT GTGAAGGTGA CAGTGGGGGA CCCTTTGTCA TGAAGAGCCC CTTTAACAAC
CGCTGGTATC AAATGGGCAT CGTCTCATGG GGTGAAGGCT GTGACCGGGA TGGGAAATAT
GGCTTCTACA CACATGTGTT CCGCCTGAAG AAGTGGATAC AGAAGGTCAT TGATCAGTTT
GGAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57660
Accession Version NM_001311257.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2015
Organism Homo sapiens (human)
Product prothrombin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA638868.1, AK303747.1, AK312965.1 and BC051332.1. Summary: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GTGTTCCTGG CTCCTCAGCA AGCACGGTCG 
CTGCTCCAGC GGGTCCGGCG AGCCAACACC TTCTTGGAGG AGGTGCGCAA GGGCAACCTG
GAGCGAGAGT GCGTGGAGGA GACGTGCAGC TACGAGGAGG CCTTCGAGGC TCTGGAGTCC
TCCACGGCTA CGGATGTGTT CTGGGCCAAG TACACAGCTT GTGAGACAGC GAGGACGCCT
CGAGATAAGC TTGCTGCATG TCTGGAAGGT AACTGTGCTG AGGGTCTGGG TACGAACTAC
CGAGGGCATG TGAACATCAC CCGGTCAGGC ATTGAGTGCC AGCTATGGAG GAGTCGCTAC
CCACATAAGC CTGAAATCAA CTCCACTACC CATCCTGGGG CCGACCTACA GGAGAATTTC
TGCCGCAACC CCGACAGCAG CACCACGGGA CCCTGGTGCT ACACTACAGA CCCCACCGTG
AGGAGGCAGG AATGCAGCAT CCCTGTCTGT GGCCAGGATC AAGTCACTGT AGCGATGACT
CCACGCTCCG AAGGCTCCAG TGTGAATCTG TCACCTCCAT TGGAGCAGTG TGTCCCTGAT
CGGGGGCAGC AGTACCAGGG GCGCCTGGCG GTGACCACAC ATGGGCTCCC CTGCCTGGCC
TGGGCCAGCG CACAGGCCAA GGCCCTGAGC AAGCACCAGG ACTTCAACTC AGCTGTGCAG
CTGGTGGAGA ACTTCTGCCG CAACCCAGAC GGGGATGAGG AGGGCGTGTG GTGCTATGTG
GCCGGGAAGC CTGGCGACTT TGGGTACTGC GACCTCAACT ATTGTGAGGA GGCCGTGGAG
GAGGAGACAG GAGATGGGCT GGATGAGGAC TCAGACAGGG CCATCGAAGG GCGTACCGCC
ACCAGTGAGT ACCAGACTTT CTTCAATCCG AGGACCTTTG GCTCGGGAGA GGCAGACTGT
GGGCTGCGAC CTCTGTTCGA GAAGAAGTCG CTGGAGGACA AAACCGAAAG AGAGCTCCTG
GAATCCTACA TCGACGGGCG CATTGTGGAG GGCTCGGATG CAGAGATCGG CATGTCACCT
TGGCAGGTGA TGCTTTTCCG GAAGAGTCCC CAGGAGCTGC TGTGTGGGGC CAGCCTCATC
AGTGACCGCT GGGTCCTCAC CGCCGCCCAC TGCCTCCTGT ACCCGCCCTG GGACAAGAAC
TTCACCGAGA ATGACCTTCT GGTGCGCATT GGCAAGCACT CCCGCACCAG GTACGAGCGA
AACATTGAAA AGATATCCAT GTTGGAAAAG ATCTACATCC ACCCCAGGTA CAACTGGCGG
GAGAACCTGG ACCGGGACAT TGCCCTGATG AAGCTGAAGA AGCCTGTTGC CTTCAGTGAC
TACATTCACC CTGTGTGTCT GCCCGACAGG GAGACGGCAG CCAGCTTGCT CCAGGCTGGA
TACAAGGGGC GGGTGACAGG CTGGGGCAAC CTGAAGGAGA CGTGGACAGC CAACGTTGGT
AAGGGGCAGC CCAGTGTCCT GCAGGTGGTG AACCTGCCCA TTGTGGAGCG GCCGGTCTGC
AAGGACTCCA CCCGGATCCG CATCACTGAC AACATGTTCT GTGCTGGTTA CAAGCCTGAT
GAAGGGAAAC GAGGGGATGC CTGTGAAGGT GACAGTGGGG GACCCTTTGT CATGAAGAGC
CCCTTTAACA ACCGCTGGTA TCAAATGGGC ATCGTCTCAT GGGGTGAAGG CTGTGACCGG
GATGGGAAAT ATGGCTTCTA CACACATGTG TTCCGCCTGA AGAAGTGGAT ACAGAAGGTC
ATTGATCAGT TTGGAGAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001298186.1
CDS71..1891
Misc Feature(1)62..64(+)
Exon (1)1..101
Gene:F2
Gene Synonym:
Exon (2)102..262
Gene:F2
Gene Synonym:
Exon (3)263..287
Gene:F2
Gene Synonym:
Exon (4)288..338
Gene:F2
Gene Synonym:
Exon (5)339..444
Gene:F2
Gene Synonym:
Exon (6)445..581
Gene:F2
Gene Synonym:
Exon (7)582..896
Gene:F2
Gene Synonym:
Exon (8)897..1025
Gene:F2
Gene Synonym:
Exon (9)1026..1152
Gene:F2
Gene Synonym:
Exon (10)1153..1320
Gene:F2
Gene Synonym:
Exon (11)1321..1494
Gene:F2
Gene Synonym:
Exon (12)1495..1676
Gene:F2
Gene Synonym:
Exon (13)1677..1747
Gene:F2
Gene Synonym:
Exon (14)1748..1991
Gene:F2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001311257
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001311257

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GTGTTCCTGG CTCCTCAGCA AGCACGGTCG 
CTGCTCCAGC GGGTCCGGCG AGCCAACACC TTCTTGGAGG AGGTGCGCAA GGGCAACCTG
GAGCGAGAGT GCGTGGAGGA GACGTGCAGC TACGAGGAGG CCTTCGAGGC TCTGGAGTCC
TCCACGGCTA CGGATGTGTT CTGGGCCAAG TACACAGCTT GTGAGACAGC GAGGACGCCT
CGAGATAAGC TTGCTGCATG TCTGGAAGGT AACTGTGCTG AGGGTCTGGG TACGAACTAC
CGAGGGCATG TGAACATCAC CCGGTCAGGC ATTGAGTGCC AGCTATGGAG GAGTCGCTAC
CCACATAAGC CTGAAATCAA CTCCACTACC CATCCTGGGG CCGACCTACA GGAGAATTTC
TGCCGCAACC CCGACAGCAG CACCACGGGA CCCTGGTGCT ACACTACAGA CCCCACCGTG
AGGAGGCAGG AATGCAGCAT CCCTGTCTGT GGCCAGGATC AAGTCACTGT AGCGATGACT
CCACGCTCCG AAGGCTCCAG TGTGAATCTG TCACCTCCAT TGGAGCAGTG TGTCCCTGAT
CGGGGGCAGC AGTACCAGGG GCGCCTGGCG GTGACCACAC ATGGGCTCCC CTGCCTGGCC
TGGGCCAGCG CACAGGCCAA GGCCCTGAGC AAGCACCAGG ACTTCAACTC AGCTGTGCAG
CTGGTGGAGA ACTTCTGCCG CAACCCAGAC GGGGATGAGG AGGGCGTGTG GTGCTATGTG
GCCGGGAAGC CTGGCGACTT TGGGTACTGC GACCTCAACT ATTGTGAGGA GGCCGTGGAG
GAGGAGACAG GAGATGGGCT GGATGAGGAC TCAGACAGGG CCATCGAAGG GCGTACCGCC
ACCAGTGAGT ACCAGACTTT CTTCAATCCG AGGACCTTTG GCTCGGGAGA GGCAGACTGT
GGGCTGCGAC CTCTGTTCGA GAAGAAGTCG CTGGAGGACA AAACCGAAAG AGAGCTCCTG
GAATCCTACA TCGACGGGCG CATTGTGGAG GGCTCGGATG CAGAGATCGG CATGTCACCT
TGGCAGGTGA TGCTTTTCCG GAAGAGTCCC CAGGAGCTGC TGTGTGGGGC CAGCCTCATC
AGTGACCGCT GGGTCCTCAC CGCCGCCCAC TGCCTCCTGT ACCCGCCCTG GGACAAGAAC
TTCACCGAGA ATGACCTTCT GGTGCGCATT GGCAAGCACT CCCGCACCAG GTACGAGCGA
AACATTGAAA AGATATCCAT GTTGGAAAAG ATCTACATCC ACCCCAGGTA CAACTGGCGG
GAGAACCTGG ACCGGGACAT TGCCCTGATG AAGCTGAAGA AGCCTGTTGC CTTCAGTGAC
TACATTCACC CTGTGTGTCT GCCCGACAGG GAGACGGCAG CCAGCTTGCT CCAGGCTGGA
TACAAGGGGC GGGTGACAGG CTGGGGCAAC CTGAAGGAGA CGTGGACAGC CAACGTTGGT
AAGGGGCAGC CCAGTGTCCT GCAGGTGGTG AACCTGCCCA TTGTGGAGCG GCCGGTCTGC
AAGGACTCCA CCCGGATCCG CATCACTGAC AACATGTTCT GTGCTGGTTA CAAGCCTGAT
GAAGGGAAAC GAGGGGATGC CTGTGAAGGT GACAGTGGGG GACCCTTTGT CATGAAGAGC
CCCTTTAACA ACCGCTGGTA TCAAATGGGC ATCGTCTCAT GGGGTGAAGG CTGTGACCGG
GATGGGAAAT ATGGCTTCTA CACACATGTG TTCCGCCTGA AGAAGTGGAT ACAGAAGGTC
ATTGATCAGT TTGGAGAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MASP-1 of the complement system promotes clotting via prothrombin activation
Mol. Immunol. 65 (2), 398-405 (2015)
Jenny L, Dobo J, Gal P and Schroeder V.


book

Why Ser and not Thr brokers catalysis in the trypsin fold
Biochemistry 54 (7), 1457-1464 (2015)
Pelc LA, Chen Z, Gohara DW, Vogt AD, Pozzi N and Di Cera E.


book

A novel congenital dysprothrombinemia leading to defective prothrombin maturation
Thromb. Res. 134 (5), 1135-1141 (2014)
Bafunno V, Bury L, Tiscia GL, Fierro T, Favuzzi G, Caliandro R, Sessa F, Grandone E, Margaglione M and Gresele P.


book

Routine coagulation tests are not useful as a screening tool for the FII G20210A polymorphism
Clin. Lab. 60 (10), 1725-1733 (2014)
Rolla,R., Pergolini,P., Vidali,M., Pollarolo,P., Appiani,A., Cerutti,C., Manzini,M., Crisci,A. and Bellomo,G.


book

Proteolysis of human thrombin generates novel host defense peptides
PLoS Pathog. 6 (4), E1000857 (2010)
Papareddy P, Rydengard V, Pasupuleti M, Walse B, Morgelin M, Chalupka A, Malmsten M and Schmidtchen A.


book

Prothrombin-Related Thrombophilia
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kujovich,J.L.


book

Molecular and genetic analysis of a compound heterozygote for dysprothrombinemia of prothrombin Tokushima and hypoprothrombinemia
Am. J. Hum. Genet. 51 (6), 1386-1395 (1992)
Iwahana H, Yoshimoto K, Shigekiyo T, Shirakami A, Saito S and Itakura M.


book

Prothrombin Quick. A newly identified dysprothrombinemia
Mayo Clin. Proc. 53 (1), 29-33 (1978)
Owen,C.A. Jr., Henriksen,R.A., McDuffie,F.C. and Mann,K.G.


book

Primary structure of human prethrombin 2 and alpha-thrombin
J. Biol. Chem. 252 (14), 4942-4957 (1977)
Butkowski,R.J., Elion,J., Downing,M.R. and Mann,K.G.


book

Amino acid sequence of human prothrombin fragments 1 and 2
Proc. Natl. Acad. Sci. U.S.A. 74 (5), 1969-1972 (1977)
Walz,D.A., Hewett-Emmett,D. and Seegers,W.H.


 
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