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F2 coagulation factor II (thrombin) [Homo sapiens (human)]

Gene Symbol F2
Entrez Gene ID 2147
Full Name coagulation factor II (thrombin)
Synonyms PT, RPRGL2, THPH1
General protein information
Preferred Names
prothrombin
Names
prothrombin
serine protease
prothrombin B-chain
prepro-coagulation factor II
NP_000497.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p11

Summary Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

176930

Disorder Html: Hypoprothrombinemia, 613679 (3); Dysprothrombinemia, 613679 (3);





The following F2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26797 NM_000506 Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379
OHu57660 NM_001311257 Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu26797
Accession Version NM_000506.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-AUG-2015
Organism Homo sapiens (human)
Product prothrombin isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA638868.1, AK312965.1, AK303747.1 and BC051332.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2015 this sequence version replaced gi:169808403. Summary: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 20421939 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTGTAGC 
CTTGTGCACA GCCAGCATGT GTTCCTGGCT CCTCAGCAAG CACGGTCGCT GCTCCAGCGG
GTCCGGCGAG CCAACACCTT CTTGGAGGAG GTGCGCAAGG GCAACCTGGA GCGAGAGTGC
GTGGAGGAGA CGTGCAGCTA CGAGGAGGCC TTCGAGGCTC TGGAGTCCTC CACGGCTACG
GATGTGTTCT GGGCCAAGTA CACAGCTTGT GAGACAGCGA GGACGCCTCG AGATAAGCTT
GCTGCATGTC TGGAAGGTAA CTGTGCTGAG GGTCTGGGTA CGAACTACCG AGGGCATGTG
AACATCACCC GGTCAGGCAT TGAGTGCCAG CTATGGAGGA GTCGCTACCC ACATAAGCCT
GAAATCAACT CCACTACCCA TCCTGGGGCC GACCTACAGG AGAATTTCTG CCGCAACCCC
GACAGCAGCA CCACGGGACC CTGGTGCTAC ACTACAGACC CCACCGTGAG GAGGCAGGAA
TGCAGCATCC CTGTCTGTGG CCAGGATCAA GTCACTGTAG CGATGACTCC ACGCTCCGAA
GGCTCCAGTG TGAATCTGTC ACCTCCATTG GAGCAGTGTG TCCCTGATCG GGGGCAGCAG
TACCAGGGGC GCCTGGCGGT GACCACACAT GGGCTCCCCT GCCTGGCCTG GGCCAGCGCA
CAGGCCAAGG CCCTGAGCAA GCACCAGGAC TTCAACTCAG CTGTGCAGCT GGTGGAGAAC
TTCTGCCGCA ACCCAGACGG GGATGAGGAG GGCGTGTGGT GCTATGTGGC CGGGAAGCCT
GGCGACTTTG GGTACTGCGA CCTCAACTAT TGTGAGGAGG CCGTGGAGGA GGAGACAGGA
GATGGGCTGG ATGAGGACTC AGACAGGGCC ATCGAAGGGC GTACCGCCAC CAGTGAGTAC
CAGACTTTCT TCAATCCGAG GACCTTTGGC TCGGGAGAGG CAGACTGTGG GCTGCGACCT
CTGTTCGAGA AGAAGTCGCT GGAGGACAAA ACCGAAAGAG AGCTCCTGGA ATCCTACATC
GACGGGCGCA TTGTGGAGGG CTCGGATGCA GAGATCGGCA TGTCACCTTG GCAGGTGATG
CTTTTCCGGA AGAGTCCCCA GGAGCTGCTG TGTGGGGCCA GCCTCATCAG TGACCGCTGG
GTCCTCACCG CCGCCCACTG CCTCCTGTAC CCGCCCTGGG ACAAGAACTT CACCGAGAAT
GACCTTCTGG TGCGCATTGG CAAGCACTCC CGCACCAGGT ACGAGCGAAA CATTGAAAAG
ATATCCATGT TGGAAAAGAT CTACATCCAC CCCAGGTACA ACTGGCGGGA GAACCTGGAC
CGGGACATTG CCCTGATGAA GCTGAAGAAG CCTGTTGCCT TCAGTGACTA CATTCACCCT
GTGTGTCTGC CCGACAGGGA GACGGCAGCC AGCTTGCTCC AGGCTGGATA CAAGGGGCGG
GTGACAGGCT GGGGCAACCT GAAGGAGACG TGGACAGCCA ACGTTGGTAA GGGGCAGCCC
AGTGTCCTGC AGGTGGTGAA CCTGCCCATT GTGGAGCGGC CGGTCTGCAA GGACTCCACC
CGGATCCGCA TCACTGACAA CATGTTCTGT GCTGGTTACA AGCCTGATGA AGGGAAACGA
GGGGATGCCT GTGAAGGTGA CAGTGGGGGA CCCTTTGTCA TGAAGAGCCC CTTTAACAAC
CGCTGGTATC AAATGGGCAT CGTCTCATGG GGTGAAGGCT GTGACCGGGA TGGGAAATAT
GGCTTCTACA CACATGTGTT CCGCCTGAAG AAGTGGATAC AGAAGGTCAT TGATCAGTTT
GGAGAGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000497.1
CDS71..1939
Misc Feature(1)62..64(+)
Misc Feature(2)143..334(+)
Misc Feature(3)215..217(+)
Misc Feature(4)218..220(+)
Misc Feature(5)239..241(+)
Misc Feature(6)245..247(+)
Misc Feature(7)254..256(+)
Misc Feature(8)257..259(+)
Misc Feature(9)272..274(+)
Misc Feature(10)275..277(+)
Misc Feature(11)284..286(+)
Misc Feature(12)293..295(+)
Misc Feature(13)383..628(+)
Misc Feature(14)419..421(+)
Misc Feature(15)431..433(+)
Misc Feature(16)464..604(+)
Misc Feature(17)497..499(+)
Misc Feature(18)662..664(+)
Misc Feature(19)701..946(+)
Misc Feature(20)734..736(+)
Misc Feature(21)779..919(+)
Misc Feature(22)1010..1012(+)
Misc Feature(23)1013..1159(+)
Misc Feature(24)1049..1051(+)
Misc Feature(25)1157..1909(+)
Misc Feature(26)1160..1918(+)
Misc Feature(27)1160..1162(+)
Misc Feature(28)1286..1774(+)
Misc Feature(29)1376..1378(+)
Misc Feature(30)1754..1843(+)
Exon (1)1..149
Gene:F2
Gene Synonym:
Exon (2)150..310
Gene:F2
Gene Synonym:
Exon (3)311..335
Gene:F2
Gene Synonym:
Exon (4)336..386
Gene:F2
Gene Synonym:
Exon (5)387..492
Gene:F2
Gene Synonym:
Exon (6)493..629
Gene:F2
Gene Synonym:
Exon (7)630..944
Gene:F2
Gene Synonym:
Exon (8)945..1073
Gene:F2
Gene Synonym:
Exon (9)1074..1200
Gene:F2
Gene Synonym:
Exon (10)1201..1368
Gene:F2
Gene Synonym:
Exon (11)1369..1542
Gene:F2
Gene Synonym:
Exon (12)1543..1724
Gene:F2
Gene Synonym:
Exon (13)1725..1795
Gene:F2
Gene Synonym:
Exon (14)1796..2039
Gene:F2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_000506
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000506

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTGTAGC 
CTTGTGCACA GCCAGCATGT GTTCCTGGCT CCTCAGCAAG CACGGTCGCT GCTCCAGCGG
GTCCGGCGAG CCAACACCTT CTTGGAGGAG GTGCGCAAGG GCAACCTGGA GCGAGAGTGC
GTGGAGGAGA CGTGCAGCTA CGAGGAGGCC TTCGAGGCTC TGGAGTCCTC CACGGCTACG
GATGTGTTCT GGGCCAAGTA CACAGCTTGT GAGACAGCGA GGACGCCTCG AGATAAGCTT
GCTGCATGTC TGGAAGGTAA CTGTGCTGAG GGTCTGGGTA CGAACTACCG AGGGCATGTG
AACATCACCC GGTCAGGCAT TGAGTGCCAG CTATGGAGGA GTCGCTACCC ACATAAGCCT
GAAATCAACT CCACTACCCA TCCTGGGGCC GACCTACAGG AGAATTTCTG CCGCAACCCC
GACAGCAGCA CCACGGGACC CTGGTGCTAC ACTACAGACC CCACCGTGAG GAGGCAGGAA
TGCAGCATCC CTGTCTGTGG CCAGGATCAA GTCACTGTAG CGATGACTCC ACGCTCCGAA
GGCTCCAGTG TGAATCTGTC ACCTCCATTG GAGCAGTGTG TCCCTGATCG GGGGCAGCAG
TACCAGGGGC GCCTGGCGGT GACCACACAT GGGCTCCCCT GCCTGGCCTG GGCCAGCGCA
CAGGCCAAGG CCCTGAGCAA GCACCAGGAC TTCAACTCAG CTGTGCAGCT GGTGGAGAAC
TTCTGCCGCA ACCCAGACGG GGATGAGGAG GGCGTGTGGT GCTATGTGGC CGGGAAGCCT
GGCGACTTTG GGTACTGCGA CCTCAACTAT TGTGAGGAGG CCGTGGAGGA GGAGACAGGA
GATGGGCTGG ATGAGGACTC AGACAGGGCC ATCGAAGGGC GTACCGCCAC CAGTGAGTAC
CAGACTTTCT TCAATCCGAG GACCTTTGGC TCGGGAGAGG CAGACTGTGG GCTGCGACCT
CTGTTCGAGA AGAAGTCGCT GGAGGACAAA ACCGAAAGAG AGCTCCTGGA ATCCTACATC
GACGGGCGCA TTGTGGAGGG CTCGGATGCA GAGATCGGCA TGTCACCTTG GCAGGTGATG
CTTTTCCGGA AGAGTCCCCA GGAGCTGCTG TGTGGGGCCA GCCTCATCAG TGACCGCTGG
GTCCTCACCG CCGCCCACTG CCTCCTGTAC CCGCCCTGGG ACAAGAACTT CACCGAGAAT
GACCTTCTGG TGCGCATTGG CAAGCACTCC CGCACCAGGT ACGAGCGAAA CATTGAAAAG
ATATCCATGT TGGAAAAGAT CTACATCCAC CCCAGGTACA ACTGGCGGGA GAACCTGGAC
CGGGACATTG CCCTGATGAA GCTGAAGAAG CCTGTTGCCT TCAGTGACTA CATTCACCCT
GTGTGTCTGC CCGACAGGGA GACGGCAGCC AGCTTGCTCC AGGCTGGATA CAAGGGGCGG
GTGACAGGCT GGGGCAACCT GAAGGAGACG TGGACAGCCA ACGTTGGTAA GGGGCAGCCC
AGTGTCCTGC AGGTGGTGAA CCTGCCCATT GTGGAGCGGC CGGTCTGCAA GGACTCCACC
CGGATCCGCA TCACTGACAA CATGTTCTGT GCTGGTTACA AGCCTGATGA AGGGAAACGA
GGGGATGCCT GTGAAGGTGA CAGTGGGGGA CCCTTTGTCA TGAAGAGCCC CTTTAACAAC
CGCTGGTATC AAATGGGCAT CGTCTCATGG GGTGAAGGCT GTGACCGGGA TGGGAAATAT
GGCTTCTACA CACATGTGTT CCGCCTGAAG AAGTGGATAC AGAAGGTCAT TGATCAGTTT
GGAGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57660
Accession Version NM_001311257.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2015
Organism Homo sapiens (human)
Product prothrombin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA638868.1, AK303747.1, AK312965.1 and BC051332.1. Summary: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GTGTTCCTGG CTCCTCAGCA AGCACGGTCG 
CTGCTCCAGC GGGTCCGGCG AGCCAACACC TTCTTGGAGG AGGTGCGCAA GGGCAACCTG
GAGCGAGAGT GCGTGGAGGA GACGTGCAGC TACGAGGAGG CCTTCGAGGC TCTGGAGTCC
TCCACGGCTA CGGATGTGTT CTGGGCCAAG TACACAGCTT GTGAGACAGC GAGGACGCCT
CGAGATAAGC TTGCTGCATG TCTGGAAGGT AACTGTGCTG AGGGTCTGGG TACGAACTAC
CGAGGGCATG TGAACATCAC CCGGTCAGGC ATTGAGTGCC AGCTATGGAG GAGTCGCTAC
CCACATAAGC CTGAAATCAA CTCCACTACC CATCCTGGGG CCGACCTACA GGAGAATTTC
TGCCGCAACC CCGACAGCAG CACCACGGGA CCCTGGTGCT ACACTACAGA CCCCACCGTG
AGGAGGCAGG AATGCAGCAT CCCTGTCTGT GGCCAGGATC AAGTCACTGT AGCGATGACT
CCACGCTCCG AAGGCTCCAG TGTGAATCTG TCACCTCCAT TGGAGCAGTG TGTCCCTGAT
CGGGGGCAGC AGTACCAGGG GCGCCTGGCG GTGACCACAC ATGGGCTCCC CTGCCTGGCC
TGGGCCAGCG CACAGGCCAA GGCCCTGAGC AAGCACCAGG ACTTCAACTC AGCTGTGCAG
CTGGTGGAGA ACTTCTGCCG CAACCCAGAC GGGGATGAGG AGGGCGTGTG GTGCTATGTG
GCCGGGAAGC CTGGCGACTT TGGGTACTGC GACCTCAACT ATTGTGAGGA GGCCGTGGAG
GAGGAGACAG GAGATGGGCT GGATGAGGAC TCAGACAGGG CCATCGAAGG GCGTACCGCC
ACCAGTGAGT ACCAGACTTT CTTCAATCCG AGGACCTTTG GCTCGGGAGA GGCAGACTGT
GGGCTGCGAC CTCTGTTCGA GAAGAAGTCG CTGGAGGACA AAACCGAAAG AGAGCTCCTG
GAATCCTACA TCGACGGGCG CATTGTGGAG GGCTCGGATG CAGAGATCGG CATGTCACCT
TGGCAGGTGA TGCTTTTCCG GAAGAGTCCC CAGGAGCTGC TGTGTGGGGC CAGCCTCATC
AGTGACCGCT GGGTCCTCAC CGCCGCCCAC TGCCTCCTGT ACCCGCCCTG GGACAAGAAC
TTCACCGAGA ATGACCTTCT GGTGCGCATT GGCAAGCACT CCCGCACCAG GTACGAGCGA
AACATTGAAA AGATATCCAT GTTGGAAAAG ATCTACATCC ACCCCAGGTA CAACTGGCGG
GAGAACCTGG ACCGGGACAT TGCCCTGATG AAGCTGAAGA AGCCTGTTGC CTTCAGTGAC
TACATTCACC CTGTGTGTCT GCCCGACAGG GAGACGGCAG CCAGCTTGCT CCAGGCTGGA
TACAAGGGGC GGGTGACAGG CTGGGGCAAC CTGAAGGAGA CGTGGACAGC CAACGTTGGT
AAGGGGCAGC CCAGTGTCCT GCAGGTGGTG AACCTGCCCA TTGTGGAGCG GCCGGTCTGC
AAGGACTCCA CCCGGATCCG CATCACTGAC AACATGTTCT GTGCTGGTTA CAAGCCTGAT
GAAGGGAAAC GAGGGGATGC CTGTGAAGGT GACAGTGGGG GACCCTTTGT CATGAAGAGC
CCCTTTAACA ACCGCTGGTA TCAAATGGGC ATCGTCTCAT GGGGTGAAGG CTGTGACCGG
GATGGGAAAT ATGGCTTCTA CACACATGTG TTCCGCCTGA AGAAGTGGAT ACAGAAGGTC
ATTGATCAGT TTGGAGAGTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001298186.1
CDS71..1891
Misc Feature(1)62..64(+)
Exon (1)1..101
Gene:F2
Gene Synonym:
Exon (2)102..262
Gene:F2
Gene Synonym:
Exon (3)263..287
Gene:F2
Gene Synonym:
Exon (4)288..338
Gene:F2
Gene Synonym:
Exon (5)339..444
Gene:F2
Gene Synonym:
Exon (6)445..581
Gene:F2
Gene Synonym:
Exon (7)582..896
Gene:F2
Gene Synonym:
Exon (8)897..1025
Gene:F2
Gene Synonym:
Exon (9)1026..1152
Gene:F2
Gene Synonym:
Exon (10)1153..1320
Gene:F2
Gene Synonym:
Exon (11)1321..1494
Gene:F2
Gene Synonym:
Exon (12)1495..1676
Gene:F2
Gene Synonym:
Exon (13)1677..1747
Gene:F2
Gene Synonym:
Exon (14)1748..1991
Gene:F2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001311257
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor II (thrombin) (F2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001311257

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGCACG TCCGAGGCTT GCAGCTGCCT GTGTTCCTGG CTCCTCAGCA AGCACGGTCG 
CTGCTCCAGC GGGTCCGGCG AGCCAACACC TTCTTGGAGG AGGTGCGCAA GGGCAACCTG
GAGCGAGAGT GCGTGGAGGA GACGTGCAGC TACGAGGAGG CCTTCGAGGC TCTGGAGTCC
TCCACGGCTA CGGATGTGTT CTGGGCCAAG TACACAGCTT GTGAGACAGC GAGGACGCCT
CGAGATAAGC TTGCTGCATG TCTGGAAGGT AACTGTGCTG AGGGTCTGGG TACGAACTAC
CGAGGGCATG TGAACATCAC CCGGTCAGGC ATTGAGTGCC AGCTATGGAG GAGTCGCTAC
CCACATAAGC CTGAAATCAA CTCCACTACC CATCCTGGGG CCGACCTACA GGAGAATTTC
TGCCGCAACC CCGACAGCAG CACCACGGGA CCCTGGTGCT ACACTACAGA CCCCACCGTG
AGGAGGCAGG AATGCAGCAT CCCTGTCTGT GGCCAGGATC AAGTCACTGT AGCGATGACT
CCACGCTCCG AAGGCTCCAG TGTGAATCTG TCACCTCCAT TGGAGCAGTG TGTCCCTGAT
CGGGGGCAGC AGTACCAGGG GCGCCTGGCG GTGACCACAC ATGGGCTCCC CTGCCTGGCC
TGGGCCAGCG CACAGGCCAA GGCCCTGAGC AAGCACCAGG ACTTCAACTC AGCTGTGCAG
CTGGTGGAGA ACTTCTGCCG CAACCCAGAC GGGGATGAGG AGGGCGTGTG GTGCTATGTG
GCCGGGAAGC CTGGCGACTT TGGGTACTGC GACCTCAACT ATTGTGAGGA GGCCGTGGAG
GAGGAGACAG GAGATGGGCT GGATGAGGAC TCAGACAGGG CCATCGAAGG GCGTACCGCC
ACCAGTGAGT ACCAGACTTT CTTCAATCCG AGGACCTTTG GCTCGGGAGA GGCAGACTGT
GGGCTGCGAC CTCTGTTCGA GAAGAAGTCG CTGGAGGACA AAACCGAAAG AGAGCTCCTG
GAATCCTACA TCGACGGGCG CATTGTGGAG GGCTCGGATG CAGAGATCGG CATGTCACCT
TGGCAGGTGA TGCTTTTCCG GAAGAGTCCC CAGGAGCTGC TGTGTGGGGC CAGCCTCATC
AGTGACCGCT GGGTCCTCAC CGCCGCCCAC TGCCTCCTGT ACCCGCCCTG GGACAAGAAC
TTCACCGAGA ATGACCTTCT GGTGCGCATT GGCAAGCACT CCCGCACCAG GTACGAGCGA
AACATTGAAA AGATATCCAT GTTGGAAAAG ATCTACATCC ACCCCAGGTA CAACTGGCGG
GAGAACCTGG ACCGGGACAT TGCCCTGATG AAGCTGAAGA AGCCTGTTGC CTTCAGTGAC
TACATTCACC CTGTGTGTCT GCCCGACAGG GAGACGGCAG CCAGCTTGCT CCAGGCTGGA
TACAAGGGGC GGGTGACAGG CTGGGGCAAC CTGAAGGAGA CGTGGACAGC CAACGTTGGT
AAGGGGCAGC CCAGTGTCCT GCAGGTGGTG AACCTGCCCA TTGTGGAGCG GCCGGTCTGC
AAGGACTCCA CCCGGATCCG CATCACTGAC AACATGTTCT GTGCTGGTTA CAAGCCTGAT
GAAGGGAAAC GAGGGGATGC CTGTGAAGGT GACAGTGGGG GACCCTTTGT CATGAAGAGC
CCCTTTAACA ACCGCTGGTA TCAAATGGGC ATCGTCTCAT GGGGTGAAGG CTGTGACCGG
GATGGGAAAT ATGGCTTCTA CACACATGTG TTCCGCCTGA AGAAGTGGAT ACAGAAGGTC
ATTGATCAGT TTGGAGAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.