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EFEMP1 EGF containing fibulin-like extracellular matrix protein 1 [Homo sapiens (human)]

Gene Symbol EFEMP1
Entrez Gene ID 2202
Full Name EGF containing fibulin-like extracellular matrix protein 1
Synonyms DHRD, DRAD, FBLN3, FBNL, FIBL-3, MLVT, MTLV, S1-5
General protein information
Preferred Names
EGF-containing fibulin-like extracellular matrix protein 1
Names
EGF-containing fibulin-like extracellular matrix protein 1
fibulin-3
extracellular protein S1-5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p16

Summary This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

601548

Disorder Html: Doyne honeycomb degeneration of retina, 126600 (3)





The following EFEMP1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFEMP1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35325 XM_005264205 PREDICTED: Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu22367 NM_001039348 Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector in pcDNA3.1+/C-(K)DYK $379
OHu22367 NM_001039349 Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector in pcDNA3.1+/C-(K)DYK $379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu35325
Accession Version XM_005264205.3
Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product EGF-containing fibulin-like extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802779. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCAACGT GTCCTCTCTG GCGTGGGGTC CCCGCGAGTC TGGGAAACGA GGAGCTGGCA 
AGGGAGGGCA AGCGGGGGCG GCAAAGAGGG CCCCTGGAGC AGGGGGCGCG GACCTCGCTG
CGCTGCGCGC TCTACCGCCG GGCTCGCAAC GCTGGGCTCA GCGCTCGCGC CTCCCTCAGC
TCTCTCCTCC GCCCCCCTTC GCCCTCCCCC TTTCCCTCCC TTTCTCCTCC TCCTCCTGCC
GCCGCGGCCG CTGCCGGACT TCGCCAGATC AGACCCACGG GGCTGCCCTC CCCTGCGCAC
TCCCCTCGCT GCCCGGGCCC GGAGCGCAGC GCGGCCGCAC AGGTATTTTT GCTGTGCTGT
GCAAGGAACT CTGCTAGCTC AAGATTCACA ATGTTGAAAG CCCTTTTCCT AACTATGCTG
ACTCTGGCGC TGGTCAAGTC ACAGGACACC GAAGAAACCA TCACGTACAC GCAATGCACT
GACGGATATG AGTGGGATCC TGTGAGACAG CAATGCAAAG ATATTGATGA ATGTGACATT
GTCCCAGACG CTTGTAAAGG TGGAATGAAG TGTGTCAACC ACTATGGAGG ATACCTCTGC
CTTCCGAAAA CAGCCCAGAT TATTGTCAAT AATGAACAGC CTCAGCAGGA AACACAACCA
GCAGAAGGAA CCTCAGGGGC AACCACCGGG GTTGTAGCTG CCAGCAGCAT GGCAACCAGT
GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC AGTGCTGCTG CAGTCGCAGG CCCTGAAATG
CAGACTGGCC GAAATAACTT TGTCATCCGG CGGAACCCAG CTGACCCTCA GCGCATTCCC
TCCAACCCTT CCCACCGTAT CCAGTGTGCA GCAGGCTACG AGCAAAGTGA ACACAACGTG
TGCCAAGACA TAGACGAGTG CACTGCAGGG ACGCACAACT GTAGAGCAGA CCAAGTGTGC
ATCAATTTAC GGGGATCCTT TGCATGTCAG TGCCCTCCTG GATATCAGAA GCGAGGGGAG
CAGTGCGTAG ACATTGATGA ATGCAGAACC TCAAGCTACC TGTGTCAATA TCAATGTGTC
AATGAACCTG GGAAATTCTC ATGTATGTGC CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA
ACATGTCAAG ATATAAATGA GTGTGAGACC ACAAATGAAT GCCGGGAGGA TGAAATGTGT
TGGAATTATC ATGGCGGCTT CCGTTGTTAT CCACGAAATC CTTGTCAAGA TCCCTACATT
CTAACACCAG AGAACCGATG TGTTTGCCCA GTCTCAAATG CCATGTGCCG AGAACTGCCC
CAGTCAATAG TCTACAAATA CATGAGCATC CGATCTGATA GGTCTGTGCC ATCAGACATC
TTCCAGATAC AGGCCACAAC TATTTATGCC AACACCATCA ATACTTTTCG GATTAAATCT
GGAAATGAAA ATGGAGAGTT CTACCTACGA CAAACAAGTC CTGTAAGTGC AATGCTTGTG
CTCGTGAAGT CATTATCAGG ACCAAGAGAA CATATCGTGG ACCTGGAGAT GCTGACAGTC
AGCAGTATAG GGACCTTCCG CACAAGCTCT GTGTTAAGAT TGACAATAAT AGTGGGGCCA
TTTTCATTTT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005264262.1
CDS112..1743
Misc Feature(1)631..>714(+)
Misc Feature(2)1018..1140(+)
Misc Feature(3)1018..1077(+)
Misc Feature(4)1141..1260(+)
Misc Feature(5)1141..1194(+)
Misc Feature(6)1261..1377(+)
Misc Feature(7)1261..1317(+)
Translation
Position Chain Variation Link
20 20 a, g dbSNP:181925896
38 38 c, t dbSNP:72811724
42 42 a, c dbSNP:79563212
102 102 a, t dbSNP:571128144
134 134 c, g dbSNP:111619737
165 165 g, t dbSNP:564600256
168 168 a, g dbSNP:749970614
186 186 a, g dbSNP:544233321
201 201 -, g dbSNP:757993601
218 218 c, t dbSNP:189647029
236 236 -, c dbSNP:560572677
237 237 -, c dbSNP:547601704
240 240 a, g dbSNP:764700171
241 241 a, c dbSNP:561867060
246 246 -, cc dbSNP:779070955
297 297 c, t dbSNP:541873530
306 306 c, t dbSNP:3762514
307 307 a, c dbSNP:775924260
313 313 c, t dbSNP:553975490
320 320 a, c, g dbSNP:551372437
321 321 c, t dbSNP:28510596
366 366 c, g dbSNP:577899468
397 397 c, t dbSNP:558071999
402 402 c, t dbSNP:537857658
414 414 c, t dbSNP:143361440
416 416 c, t dbSNP:139902612
426 426 c, g dbSNP:535582672
435 435 a, g dbSNP:3762515
438 438 c, t dbSNP:3762516
442 442 a, g dbSNP:192789765
450 450 a, g dbSNP:10167115
456 456 -, t dbSNP:35004735
463 463 a, c dbSNP:144127545
475 475 -, g dbSNP:35393035
486 486 a, t dbSNP:187417918
498 498 a, c dbSNP:778134258
509 509 a, g dbSNP:758900931
511 511 a, g dbSNP:753247102
513 513 c, t dbSNP:539120761
517 517 c, t dbSNP:755079916
519 519 c, t dbSNP:182486751
520 520 c, g dbSNP:766388738
532 532 a, t dbSNP:760892091
534 534 c, t dbSNP:750610629
540 540 g, t dbSNP:767292073
546 546 c, t dbSNP:761653279
547 547 a, c dbSNP:150463947
570 570 c, t dbSNP:764056968
581 581 c, t dbSNP:768601184
594 594 c, t dbSNP:141747986
614 614 a, t dbSNP:763891398
619 619 a, c dbSNP:374767112
620 620 a, g dbSNP:372649180
626 626 a, g dbSNP:775356398
628 628 a, c dbSNP:371658999
635 635 c, t dbSNP:746396165
639 639 c, t dbSNP:138017183
647 647 a, c dbSNP:55849640
654 654 c, t dbSNP:747221429
660 660 c, t dbSNP:17047322
670 670 a, g dbSNP:758184486
681 681 g, t dbSNP:752397344
696 696 c, t dbSNP:142572513
708 708 a, c dbSNP:12292
716 716 c, t dbSNP:753826959
717 717 a, g, t dbSNP:376553875
735 735 c, t dbSNP:202147132
740 740 a, g dbSNP:750074607
742 742 a, g dbSNP:767113923
743 743 a, g dbSNP:761555796
747 747 a, g dbSNP:35447389
751 751 c, t dbSNP:770207315
768 768 a, g dbSNP:373374071
769 769 c, t dbSNP:777180494
774 774 a, g dbSNP:770978738
776 776 a, c dbSNP:747133568
782 782 a, c dbSNP:777942411
794 794 c, t dbSNP:772140491
797 797 c, t dbSNP:748419347
798 798 -, g dbSNP:35441215
798 798 c, t dbSNP:142637468
799 799 a, g dbSNP:150201810
802 802 g, t dbSNP:753810615
809 809 c, t dbSNP:780187035
816 816 c, t dbSNP:755653824
818 818 g, t dbSNP:749910886
819 819 c, g dbSNP:369150030
826 826 a, g dbSNP:143704611
827 827 a, c dbSNP:756800857
836 836 c, t dbSNP:751294000
840 840 g, t dbSNP:200070678
843 843 c, g dbSNP:765506968
847 847 a, g dbSNP:147918399
858 858 a, g dbSNP:777092611
876 876 c, t dbSNP:766834306
877 877 a, g dbSNP:373554215
882 882 c, t dbSNP:540635668
888 888 a, g dbSNP:14282
902 902 a, g dbSNP:748310171
908 908 a, c dbSNP:773783473
912 912 c, g, t dbSNP:768547475
916 916 a, g dbSNP:749133035
919 919 a, c, t dbSNP:756065296
920 920 a, g dbSNP:745831485
927 927 a, c, t dbSNP:756850765
930 930 a, g dbSNP:368195380
941 941 a, g dbSNP:763849216
944 944 a, g dbSNP:374747361
946 946 a, g dbSNP:758078998
968 968 a, g dbSNP:370831953
974 974 a, g dbSNP:766748201
990 990 c, t dbSNP:761107410
999 999 c, t dbSNP:773832373
1006 1006 -, aac dbSNP:764211158
1008 1008 c, t dbSNP:149890382
1024 1024 a, g dbSNP:184503723
1026 1026 c, t dbSNP:151171192
1029 1029 a, g dbSNP:769690555
1032 1032 c, t dbSNP:759416129
1035 1035 c, t dbSNP:776323409
1037 1037 c, t dbSNP:770855142
1040 1040 a, g dbSNP:199946928
1043 1043 c, t dbSNP:573326655
1044 1044 a, g dbSNP:568272529
1045 1045 c, t dbSNP:747781639
1058 1058 a, c dbSNP:778582930
1059 1059 a, g dbSNP:756400498
1062 1062 a, c dbSNP:750758098
1063 1063 c, g dbSNP:781431276
1081 1081 c, t dbSNP:398123542
1082 1082 a, g dbSNP:752116761
1089 1089 c, t dbSNP:764264501
1093 1093 a, g dbSNP:763174938
1095 1095 a, t dbSNP:752936749
1116 1116 c, t dbSNP:765517862
1124 1124 a, g dbSNP:533940631
1126 1126 g, t dbSNP:776431922
1127 1127 c, g dbSNP:770640062
1128 1128 a, g dbSNP:373664722
1129 1129 a, g dbSNP:773196469
1137 1137 c, t dbSNP:771544911
1138 1138 a, g dbSNP:369629631
1145 1145 c, t dbSNP:373857382
1155 1155 c, t dbSNP:374256179
1173 1173 a, g dbSNP:143083501
1183 1183 c, g dbSNP:774525572
1185 1185 a, g dbSNP:146846635
1188 1188 c, t dbSNP:556410164
1215 1215 c, t dbSNP:148177279
1216 1216 a, g dbSNP:763367920
1224 1224 c, g, t dbSNP:769842736
1227 1227 a, g dbSNP:745988780
1237 1237 c, g dbSNP:776713948
1242 1242 a, g dbSNP:770999988
1244 1244 g, t dbSNP:747258798
1248 1248 c, t dbSNP:777424675
1250 1250 a, g dbSNP:150399072
1264 1264 a, g dbSNP:754909186
1265 1265 c, t dbSNP:749245292
1277 1277 a, g dbSNP:779708532
1293 1293 c, t dbSNP:755710198
1294 1294 c, t dbSNP:121434491
1295 1295 a, g dbSNP:750022923
1297 1297 a, g dbSNP:576071059
1311 1311 c, t dbSNP:756890736
1332 1332 c, t dbSNP:753001473
1333 1333 c, t dbSNP:765589888
1334 1334 a, g dbSNP:372748426
1346 1346 a, g dbSNP:369049569
1357 1357 a, c dbSNP:777178356
1367 1367 a, g dbSNP:766343862
1368 1368 c, t dbSNP:760845391
1369 1369 a, c, g dbSNP:772284064
1379 1379 c, t dbSNP:762143333
1380 1380 a, g dbSNP:148823921
1381 1381 c, g dbSNP:774225644
1382 1382 a, g dbSNP:762613133
1388 1388 a, g dbSNP:779233876
1411 1411 c, g dbSNP:367901228
1416 1416 a, g dbSNP:754310502
1419 1419 c, t dbSNP:766866113
1421 1421 a, g dbSNP:146446706
1426 1426 a, c dbSNP:750455273
1451 1451 a, g dbSNP:767503998
1460 1460 c, t dbSNP:762126695
1463 1463 a, g, t dbSNP:775080680
1471 1471 a, g dbSNP:567386311
1472 1472 c, g dbSNP:139142559
1482 1482 a, g dbSNP:775180017
1513 1513 a, g dbSNP:142194873
1514 1514 g, t dbSNP:547851604
1535 1535 c, t dbSNP:780161368
1536 1536 c, t dbSNP:780748234
1541 1541 a, g dbSNP:776362721
1556 1556 a, g dbSNP:770484375
1557 1557 c, t dbSNP:534909303
1580 1580 a, g dbSNP:373990093
1581 1581 a, g dbSNP:777439665
1582 1582 c, g dbSNP:771649644
1585 1585 a, g dbSNP:747755796
1587 1587 a, g dbSNP:780466116
1597 1597 a, c dbSNP:533628546
1600 1600 g, t dbSNP:746366927
1603 1603 a, g dbSNP:781775380
1609 1609 a, g dbSNP:374643957
1611 1611 a, g, t dbSNP:147448993
1612 1612 c, t dbSNP:751641246
1615 1615 a, g dbSNP:139171666
1626 1626 a, g dbSNP:758641385
1629 1629 a, g dbSNP:149616551
1646 1646 c, t dbSNP:765057042
1647 1647 c, t dbSNP:759287964
1648 1648 a, g dbSNP:367718707
1673 1673 a, g dbSNP:766253306
1674 1674 c, t dbSNP:374690853
1679 1679 c, t dbSNP:772639652
1681 1681 c, g dbSNP:200959130
1691 1691 a, g dbSNP:201035117
1693 1693 a, c dbSNP:150488038
1694 1694 c, t dbSNP:770166720
1704 1704 g, t dbSNP:746975511
1724 1724 c, t dbSNP:746180588
1736 1736 c, t dbSNP:781708447
1739 1739 c, t dbSNP:112871166
1749 1749 -, t dbSNP:200536754
1752 1752 a, g dbSNP:199663914
1810 1810 a, t dbSNP:150344322
1816 1816 a, t dbSNP:541952588
1830 1830 a, g dbSNP:529125316
1843 1843 c, t dbSNP:113974409
1868 1868 g, t dbSNP:560047270
1889 1889 a, t dbSNP:540522685
1905 1905 c, t dbSNP:577486140
1911 1911 a, g dbSNP:1802574
1916 1916 c, t dbSNP:779068277
1925 1925 c, g dbSNP:755194729
1991 1991 a, c dbSNP:117386259
1992 1992 c, t dbSNP:373737354
1998 1998 a, g dbSNP:780676062
2000 2000 c, g dbSNP:181180919
2009 2009 c, t dbSNP:754725762
2023 2023 c, t dbSNP:535192173
2025 2025 c, t dbSNP:567064404
2026 2026 a, g dbSNP:553246404
2029 2029 a, g dbSNP:540160557
2078 2078 a, g dbSNP:571127990
2091 2091 c, t dbSNP:780507817
2093 2093 c, g dbSNP:780488845
2132 2132 c, t dbSNP:375379036
2138 2138 a, g dbSNP:571754691
2147 2147 c, g dbSNP:751018485
2188 2188 a, t dbSNP:765924429
2195 2195 -, t dbSNP:35704713
2204 2204 a, g dbSNP:1061326
2229 2229 a, g dbSNP:757690325
2245 2245 a, c dbSNP:530970873
2250 2250 c, t dbSNP:188698134
2276 2276 c, t dbSNP:56411268
2300 2300 c, t dbSNP:1063452
2317 2317 a, g dbSNP:548670062
2324 2324 c, t dbSNP:764390546
2325 2325 -, t dbSNP:55878434
2341 2341 g, t dbSNP:756425823
2392 2392 c, t dbSNP:186888998
2411 2411 c, t dbSNP:573892776
2430 2430 c, t dbSNP:138843899
2438 2438 c, t dbSNP:3791680
2439 2439 c, g dbSNP:181823941
2453 2453 c, t dbSNP:539540129
2543 2543 c, t dbSNP:146101049
2546 2546 a, c dbSNP:112572819
2673 2673 a, c dbSNP:762505852
2690 2690 a, g dbSNP:574934469
2705 2705 a, c dbSNP:77386452
2706 2706 a, g dbSNP:190519603
2736 2736 a, c dbSNP:185614902
2747 2747 c, g dbSNP:1802575
2754 2754 a, g dbSNP:568837686
2769 2769 c, t dbSNP:540075808
2805 2805 c, t dbSNP:142276628
2806 2806 a, g dbSNP:112334283
2808 2808 a, g dbSNP:147551451
2820 2820 a, g dbSNP:568443337
2836 2836 c, t dbSNP:548260378

Target ORF information:

RefSeq Version XM_005264205
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264205

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCAACGT GTCCTCTCTG GCGTGGGGTC CCCGCGAGTC TGGGAAACGA GGAGCTGGCA 
AGGGAGGGCA AGCGGGGGCG GCAAAGAGGG CCCCTGGAGC AGGGGGCGCG GACCTCGCTG
CGCTGCGCGC TCTACCGCCG GGCTCGCAAC GCTGGGCTCA GCGCTCGCGC CTCCCTCAGC
TCTCTCCTCC GCCCCCCTTC GCCCTCCCCC TTTCCCTCCC TTTCTCCTCC TCCTCCTGCC
GCCGCGGCCG CTGCCGGACT TCGCCAGATC AGACCCACGG GGCTGCCCTC CCCTGCGCAC
TCCCCTCGCT GCCCGGGCCC GGAGCGCAGC GCGGCCGCAC AGGTATTTTT GCTGTGCTGT
GCAAGGAACT CTGCTAGCTC AAGATTCACA ATGTTGAAAG CCCTTTTCCT AACTATGCTG
ACTCTGGCGC TGGTCAAGTC ACAGGACACC GAAGAAACCA TCACGTACAC GCAATGCACT
GACGGATATG AGTGGGATCC TGTGAGACAG CAATGCAAAG ATATTGATGA ATGTGACATT
GTCCCAGACG CTTGTAAAGG TGGAATGAAG TGTGTCAACC ACTATGGAGG ATACCTCTGC
CTTCCGAAAA CAGCCCAGAT TATTGTCAAT AATGAACAGC CTCAGCAGGA AACACAACCA
GCAGAAGGAA CCTCAGGGGC AACCACCGGG GTTGTAGCTG CCAGCAGCAT GGCAACCAGT
GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC AGTGCTGCTG CAGTCGCAGG CCCTGAAATG
CAGACTGGCC GAAATAACTT TGTCATCCGG CGGAACCCAG CTGACCCTCA GCGCATTCCC
TCCAACCCTT CCCACCGTAT CCAGTGTGCA GCAGGCTACG AGCAAAGTGA ACACAACGTG
TGCCAAGACA TAGACGAGTG CACTGCAGGG ACGCACAACT GTAGAGCAGA CCAAGTGTGC
ATCAATTTAC GGGGATCCTT TGCATGTCAG TGCCCTCCTG GATATCAGAA GCGAGGGGAG
CAGTGCGTAG ACATTGATGA ATGCAGAACC TCAAGCTACC TGTGTCAATA TCAATGTGTC
AATGAACCTG GGAAATTCTC ATGTATGTGC CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA
ACATGTCAAG ATATAAATGA GTGTGAGACC ACAAATGAAT GCCGGGAGGA TGAAATGTGT
TGGAATTATC ATGGCGGCTT CCGTTGTTAT CCACGAAATC CTTGTCAAGA TCCCTACATT
CTAACACCAG AGAACCGATG TGTTTGCCCA GTCTCAAATG CCATGTGCCG AGAACTGCCC
CAGTCAATAG TCTACAAATA CATGAGCATC CGATCTGATA GGTCTGTGCC ATCAGACATC
TTCCAGATAC AGGCCACAAC TATTTATGCC AACACCATCA ATACTTTTCG GATTAAATCT
GGAAATGAAA ATGGAGAGTT CTACCTACGA CAAACAAGTC CTGTAAGTGC AATGCTTGTG
CTCGTGAAGT CATTATCAGG ACCAAGAGAA CATATCGTGG ACCTGGAGAT GCTGACAGTC
AGCAGTATAG GGACCTTCCG CACAAGCTCT GTGTTAAGAT TGACAATAAT AGTGGGGCCA
TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22367
Accession Version NM_001039348.2
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product EGF-containing fibulin-like extracellular matrix protein 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA264704.1, AK312639.1, AK290599.1, AC010895.8 and U03877.1. This sequence is a reference standard in the RefSeqGene project. On Jan 27, 2011 this sequence version replaced gi:86788014. Summary: This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]. Transcript Variant: This variant (2) represents the longer transcript. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12242346) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC098561.1, AK290599.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001034437.1
CDS502..1983
Misc Feature(1)19..21(+)
Misc Feature(2)1018..1140(+)
Misc Feature(3)1018..1077(+)
Misc Feature(4)1141..1236(+)
Misc Feature(5)1141..1191(+)
Misc Feature(6)1255..1374(+)
Misc Feature(7)1276..1980(+)
Misc Feature(8)1321..1392(+)
Misc Feature(9)1375..1473(+)
Misc Feature(10)1501..>1578(+)
Exon (1)1..453
Gene:EFEMP1
Gene Synonym:
Exon (2)454..494
Gene:EFEMP1
Gene Synonym:
Exon (3)495..582
Gene:EFEMP1
Gene Synonym:
Exon (4)583..631
Gene:EFEMP1
Gene Synonym:
Exon (5)632..1018
Gene:EFEMP1
Gene Synonym:
Exon (6)1019..1141
Gene:EFEMP1
Gene Synonym:
Exon (7)1142..1261
Gene:EFEMP1
Gene Synonym:
Exon (8)1262..1381
Gene:EFEMP1
Gene Synonym:
Exon (9)1382..1501
Gene:EFEMP1
Gene Synonym:
Exon (10)1502..1625
Gene:EFEMP1
Gene Synonym:
Exon (11)1626..1821
Gene:EFEMP1
Gene Synonym:
Exon (12)1822..3094
Gene:EFEMP1
Gene Synonym:
Translation
Position Chain Variation Link
20 20 a, g dbSNP:181925896
38 38 c, t dbSNP:72811724
42 42 a, c dbSNP:79563212
102 102 a, t dbSNP:571128144
134 134 c, g dbSNP:111619737
165 165 g, t dbSNP:564600256
168 168 a, g dbSNP:749970614
186 186 a, g dbSNP:544233321
201 201 -, g dbSNP:757993601
218 218 c, t dbSNP:189647029
236 236 -, c dbSNP:560572677
237 237 -, c dbSNP:547601704
240 240 a, g dbSNP:764700171
241 241 a, c dbSNP:561867060
246 246 -, cc dbSNP:779070955
297 297 c, t dbSNP:541873530
306 306 c, t dbSNP:3762514
307 307 a, c dbSNP:775924260
313 313 c, t dbSNP:553975490
320 320 a, c, g dbSNP:551372437
321 321 c, t dbSNP:28510596
366 366 c, g dbSNP:577899468
397 397 c, t dbSNP:558071999
402 402 c, t dbSNP:537857658
414 414 c, t dbSNP:143361440
416 416 c, t dbSNP:139902612
426 426 c, g dbSNP:535582672
435 435 a, g dbSNP:3762515
438 438 c, t dbSNP:3762516
442 442 a, g dbSNP:192789765
450 450 a, g dbSNP:10167115
456 456 -, t dbSNP:35004735
463 463 a, c dbSNP:144127545
475 475 -, g dbSNP:35393035
486 486 a, t dbSNP:187417918
498 498 a, c dbSNP:778134258
509 509 a, g dbSNP:758900931
511 511 a, g dbSNP:753247102
513 513 c, t dbSNP:539120761
517 517 c, t dbSNP:755079916
519 519 c, t dbSNP:182486751
520 520 c, g dbSNP:766388738
532 532 a, t dbSNP:760892091
534 534 c, t dbSNP:750610629
540 540 g, t dbSNP:767292073
546 546 c, t dbSNP:761653279
547 547 a, c dbSNP:150463947
570 570 c, t dbSNP:764056968
581 581 c, t dbSNP:768601184
594 594 c, t dbSNP:141747986
614 614 a, t dbSNP:763891398
619 619 a, c dbSNP:374767112
620 620 a, g dbSNP:372649180
626 626 a, g dbSNP:775356398
628 628 a, c dbSNP:371658999
635 635 c, t dbSNP:746396165
639 639 c, t dbSNP:138017183
647 647 a, c dbSNP:55849640
654 654 c, t dbSNP:747221429
660 660 c, t dbSNP:17047322
670 670 a, g dbSNP:758184486
681 681 g, t dbSNP:752397344
696 696 c, t dbSNP:142572513
708 708 a, c dbSNP:12292
716 716 c, t dbSNP:753826959
717 717 a, g, t dbSNP:376553875
735 735 c, t dbSNP:202147132
740 740 a, g dbSNP:750074607
742 742 a, g dbSNP:767113923
743 743 a, g dbSNP:761555796
747 747 a, g dbSNP:35447389
751 751 c, t dbSNP:770207315
768 768 a, g dbSNP:373374071
769 769 c, t dbSNP:777180494
774 774 a, g dbSNP:770978738
776 776 a, c dbSNP:747133568
782 782 a, c dbSNP:777942411
794 794 c, t dbSNP:772140491
797 797 c, t dbSNP:748419347
798 798 -, g dbSNP:35441215
798 798 c, t dbSNP:142637468
799 799 a, g dbSNP:150201810
802 802 g, t dbSNP:753810615
809 809 c, t dbSNP:780187035
816 816 c, t dbSNP:755653824
818 818 g, t dbSNP:749910886
819 819 c, g dbSNP:369150030
826 826 a, g dbSNP:143704611
827 827 a, c dbSNP:756800857
836 836 c, t dbSNP:751294000
840 840 g, t dbSNP:200070678
843 843 c, g dbSNP:765506968
847 847 a, g dbSNP:147918399
858 858 a, g dbSNP:777092611
876 876 c, t dbSNP:766834306
877 877 a, g dbSNP:373554215
882 882 c, t dbSNP:540635668
888 888 a, g dbSNP:14282
902 902 a, g dbSNP:748310171
908 908 a, c dbSNP:773783473
912 912 c, g, t dbSNP:768547475
916 916 a, g dbSNP:749133035
919 919 a, c, t dbSNP:756065296
920 920 a, g dbSNP:745831485
927 927 a, c, t dbSNP:756850765
930 930 a, g dbSNP:368195380
941 941 a, g dbSNP:763849216
944 944 a, g dbSNP:374747361
946 946 a, g dbSNP:758078998
968 968 a, g dbSNP:370831953
974 974 a, g dbSNP:766748201
990 990 c, t dbSNP:761107410
999 999 c, t dbSNP:773832373
1006 1006 -, aac dbSNP:764211158
1008 1008 c, t dbSNP:149890382
1024 1024 a, g dbSNP:184503723
1026 1026 c, t dbSNP:151171192
1029 1029 a, g dbSNP:769690555
1032 1032 c, t dbSNP:759416129
1035 1035 c, t dbSNP:776323409
1037 1037 c, t dbSNP:770855142
1040 1040 a, g dbSNP:199946928
1043 1043 c, t dbSNP:573326655
1044 1044 a, g dbSNP:568272529
1045 1045 c, t dbSNP:747781639
1058 1058 a, c dbSNP:778582930
1059 1059 a, g dbSNP:756400498
1062 1062 a, c dbSNP:750758098
1063 1063 c, g dbSNP:781431276
1081 1081 c, t dbSNP:398123542
1082 1082 a, g dbSNP:752116761
1089 1089 c, t dbSNP:764264501
1093 1093 a, g dbSNP:763174938
1095 1095 a, t dbSNP:752936749
1116 1116 c, t dbSNP:765517862
1124 1124 a, g dbSNP:533940631
1126 1126 g, t dbSNP:776431922
1127 1127 c, g dbSNP:770640062
1128 1128 a, g dbSNP:373664722
1129 1129 a, g dbSNP:773196469
1137 1137 c, t dbSNP:771544911
1138 1138 a, g dbSNP:369629631
1159 1159 a, t dbSNP:748965004
1160 1160 a, t dbSNP:775063184
1163 1163 c, t dbSNP:771296631
1176 1176 c, t dbSNP:371553398
1185 1185 c, t dbSNP:139930646
1186 1186 a, g dbSNP:758919353
1191 1191 c, t dbSNP:539328785
1194 1194 a, g dbSNP:779048381
1197 1197 a, g dbSNP:755074953
1215 1215 g, t dbSNP:754072239
1219 1219 a, c dbSNP:576613499
1222 1222 a, c dbSNP:756356313
1224 1224 c, t dbSNP:750167724
1233 1233 a, g dbSNP:199622147
1251 1251 c, t dbSNP:761669796
1257 1257 c, t dbSNP:536279337
1258 1258 a, g dbSNP:142542256
1261 1261 g, t dbSNP:762418853
1264 1264 a, g dbSNP:764846730
1280 1280 c, t dbSNP:759260191
1284 1284 c, t dbSNP:776487850
1286 1286 a, g dbSNP:148488868
1290 1290 a, g dbSNP:762128597
1308 1308 c, t dbSNP:138120227
1353 1353 g, t dbSNP:768998905
1377 1377 c, t dbSNP:749818060
1385 1385 c, t dbSNP:373857382
1395 1395 c, t dbSNP:374256179
1413 1413 a, g dbSNP:143083501
1423 1423 c, g dbSNP:774525572
1425 1425 a, g dbSNP:146846635
1428 1428 c, t dbSNP:556410164
1455 1455 c, t dbSNP:148177279
1456 1456 a, g dbSNP:763367920
1464 1464 c, g, t dbSNP:769842736
1467 1467 a, g dbSNP:745988780
1477 1477 c, g dbSNP:776713948
1482 1482 a, g dbSNP:770999988
1484 1484 g, t dbSNP:747258798
1488 1488 c, t dbSNP:777424675
1490 1490 a, g dbSNP:150399072
1504 1504 a, g dbSNP:754909186
1505 1505 c, t dbSNP:749245292
1517 1517 a, g dbSNP:779708532
1533 1533 c, t dbSNP:755710198
1534 1534 c, t dbSNP:121434491
1535 1535 a, g dbSNP:750022923
1537 1537 a, g dbSNP:576071059
1551 1551 c, t dbSNP:756890736
1572 1572 c, t dbSNP:753001473
1573 1573 c, t dbSNP:765589888
1574 1574 a, g dbSNP:372748426
1586 1586 a, g dbSNP:369049569
1597 1597 a, c dbSNP:777178356
1607 1607 a, g dbSNP:766343862
1608 1608 c, t dbSNP:760845391
1609 1609 a, c, g dbSNP:772284064
1619 1619 c, t dbSNP:762143333
1620 1620 a, g dbSNP:148823921
1621 1621 c, g dbSNP:774225644
1622 1622 a, g dbSNP:762613133
1628 1628 a, g dbSNP:779233876
1651 1651 c, g dbSNP:367901228
1656 1656 a, g dbSNP:754310502
1659 1659 c, t dbSNP:766866113
1661 1661 a, g dbSNP:146446706
1666 1666 a, c dbSNP:750455273
1691 1691 a, g dbSNP:767503998
1700 1700 c, t dbSNP:762126695
1703 1703 a, g, t dbSNP:775080680
1711 1711 a, g dbSNP:567386311
1712 1712 c, g dbSNP:139142559
1722 1722 a, g dbSNP:775180017
1753 1753 a, g dbSNP:142194873
1754 1754 g, t dbSNP:547851604
1775 1775 c, t dbSNP:780161368
1776 1776 c, t dbSNP:780748234
1781 1781 a, g dbSNP:776362721
1796 1796 a, g dbSNP:770484375
1797 1797 c, t dbSNP:534909303
1820 1820 a, g dbSNP:373990093
1821 1821 a, g dbSNP:777439665
1822 1822 c, g dbSNP:771649644
1825 1825 a, g dbSNP:747755796
1827 1827 a, g dbSNP:780466116
1837 1837 a, c dbSNP:533628546
1840 1840 g, t dbSNP:746366927
1843 1843 a, g dbSNP:781775380
1849 1849 a, g dbSNP:374643957
1851 1851 a, g, t dbSNP:147448993
1852 1852 c, t dbSNP:751641246
1855 1855 a, g dbSNP:139171666
1866 1866 a, g dbSNP:758641385
1869 1869 a, g dbSNP:149616551
1886 1886 c, t dbSNP:765057042
1887 1887 c, t dbSNP:759287964
1888 1888 a, g dbSNP:367718707
1913 1913 a, g dbSNP:766253306
1914 1914 c, t dbSNP:374690853
1919 1919 c, t dbSNP:772639652
1921 1921 c, g dbSNP:200959130
1931 1931 a, g dbSNP:201035117
1933 1933 a, c dbSNP:150488038
1934 1934 c, t dbSNP:770166720
1944 1944 g, t dbSNP:746975511
1964 1964 c, t dbSNP:746180588
1976 1976 c, t dbSNP:781708447
1979 1979 c, t dbSNP:112871166
1989 1989 -, t dbSNP:200536754
1992 1992 a, g dbSNP:199663914
2050 2050 a, t dbSNP:150344322
2056 2056 a, t dbSNP:541952588
2070 2070 a, g dbSNP:529125316
2083 2083 c, t dbSNP:113974409
2108 2108 g, t dbSNP:560047270
2129 2129 a, t dbSNP:540522685
2145 2145 c, t dbSNP:577486140
2151 2151 a, g dbSNP:1802574
2156 2156 c, t dbSNP:779068277
2165 2165 c, g dbSNP:755194729
2231 2231 a, c dbSNP:117386259
2232 2232 c, t dbSNP:373737354
2238 2238 a, g dbSNP:780676062
2240 2240 c, g dbSNP:181180919
2249 2249 c, t dbSNP:754725762
2263 2263 c, t dbSNP:535192173
2265 2265 c, t dbSNP:567064404
2266 2266 a, g dbSNP:553246404
2269 2269 a, g dbSNP:540160557
2318 2318 a, g dbSNP:571127990
2331 2331 c, t dbSNP:780507817
2333 2333 c, g dbSNP:780488845
2372 2372 c, t dbSNP:375379036
2378 2378 a, g dbSNP:571754691
2387 2387 c, g dbSNP:751018485
2428 2428 a, t dbSNP:765924429
2435 2435 -, t dbSNP:35704713
2444 2444 a, g dbSNP:1061326
2469 2469 a, g dbSNP:757690325
2485 2485 a, c dbSNP:530970873
2490 2490 c, t dbSNP:188698134
2516 2516 c, t dbSNP:56411268
2540 2540 c, t dbSNP:1063452
2557 2557 a, g dbSNP:548670062
2564 2564 c, t dbSNP:764390546
2565 2565 -, t dbSNP:55878434
2581 2581 g, t dbSNP:756425823
2632 2632 c, t dbSNP:186888998
2651 2651 c, t dbSNP:573892776
2670 2670 c, t dbSNP:138843899
2678 2678 c, t dbSNP:3791680
2679 2679 c, g dbSNP:181823941
2693 2693 c, t dbSNP:539540129
2783 2783 c, t dbSNP:146101049
2786 2786 a, c dbSNP:112572819
2913 2913 a, c dbSNP:762505852
2930 2930 a, g dbSNP:574934469
2945 2945 a, c dbSNP:77386452
2946 2946 a, g dbSNP:190519603
2976 2976 a, c dbSNP:185614902
2987 2987 c, g dbSNP:1802575
2994 2994 a, g dbSNP:568837686
3009 3009 c, t dbSNP:540075808
3045 3045 c, t dbSNP:142276628
3046 3046 a, g dbSNP:112334283
3048 3048 a, g dbSNP:147551451
3060 3060 a, g dbSNP:568443337
3076 3076 c, t dbSNP:548260378
3094 3094 a, g dbSNP:192552476

Target ORF information:

RefSeq Version NM_001039348
Organism Homo sapiens (human)
Definition Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039348

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22367
Accession Version NM_001039349.2
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product EGF-containing fibulin-like extracellular matrix protein 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA264704.1, BC014410.1, AC010895.8 and U03877.1. On Jan 27, 2011 this sequence version replaced gi:86788131. Summary: This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 2. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293058.1, BC014410.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001034438.1
CDS461..1942
Misc Feature(1)65..67(+)
Misc Feature(2)977..1099(+)
Misc Feature(3)977..1036(+)
Misc Feature(4)1100..1195(+)
Misc Feature(5)1100..1150(+)
Misc Feature(6)1214..1333(+)
Misc Feature(7)1235..1939(+)
Misc Feature(8)1280..1351(+)
Misc Feature(9)1334..1432(+)
Misc Feature(10)1460..>1537(+)
Exon (1)1..453
Gene:EFEMP1
Gene Synonym:
Exon (2)454..541
Gene:EFEMP1
Gene Synonym:
Exon (3)542..590
Gene:EFEMP1
Gene Synonym:
Exon (4)591..977
Gene:EFEMP1
Gene Synonym:
Exon (5)978..1100
Gene:EFEMP1
Gene Synonym:
Exon (6)1101..1220
Gene:EFEMP1
Gene Synonym:
Exon (7)1221..1340
Gene:EFEMP1
Gene Synonym:
Exon (8)1341..1460
Gene:EFEMP1
Gene Synonym:
Exon (9)1461..1584
Gene:EFEMP1
Gene Synonym:
Exon (10)1585..1780
Gene:EFEMP1
Gene Synonym:
Exon (11)1781..3053
Gene:EFEMP1
Gene Synonym:
Translation
Position Chain Variation Link
20 20 a, g dbSNP:181925896
38 38 c, t dbSNP:72811724
42 42 a, c dbSNP:79563212
102 102 a, t dbSNP:571128144
134 134 c, g dbSNP:111619737
165 165 g, t dbSNP:564600256
168 168 a, g dbSNP:749970614
186 186 a, g dbSNP:544233321
201 201 -, g dbSNP:757993601
218 218 c, t dbSNP:189647029
236 236 -, c dbSNP:560572677
237 237 -, c dbSNP:547601704
240 240 a, g dbSNP:764700171
241 241 a, c dbSNP:561867060
246 246 -, cc dbSNP:779070955
297 297 c, t dbSNP:541873530
306 306 c, t dbSNP:3762514
307 307 a, c dbSNP:775924260
313 313 c, t dbSNP:553975490
320 320 a, c, g dbSNP:551372437
321 321 c, t dbSNP:28510596
366 366 c, g dbSNP:577899468
397 397 c, t dbSNP:558071999
402 402 c, t dbSNP:537857658
414 414 c, t dbSNP:143361440
416 416 c, t dbSNP:139902612
426 426 c, g dbSNP:535582672
435 435 a, g dbSNP:3762515
438 438 c, t dbSNP:3762516
442 442 a, g dbSNP:192789765
450 450 a, g dbSNP:10167115
457 457 a, c dbSNP:778134258
468 468 a, g dbSNP:758900931
470 470 a, g dbSNP:753247102
472 472 c, t dbSNP:539120761
476 476 c, t dbSNP:755079916
478 478 c, t dbSNP:182486751
479 479 c, g dbSNP:766388738
491 491 a, t dbSNP:760892091
493 493 c, t dbSNP:750610629
499 499 g, t dbSNP:767292073
505 505 c, t dbSNP:761653279
506 506 a, c dbSNP:150463947
529 529 c, t dbSNP:764056968
540 540 c, t dbSNP:768601184
553 553 c, t dbSNP:141747986
573 573 a, t dbSNP:763891398
578 578 a, c dbSNP:374767112
579 579 a, g dbSNP:372649180
585 585 a, g dbSNP:775356398
587 587 a, c dbSNP:371658999
594 594 c, t dbSNP:746396165
598 598 c, t dbSNP:138017183
606 606 a, c dbSNP:55849640
613 613 c, t dbSNP:747221429
619 619 c, t dbSNP:17047322
629 629 a, g dbSNP:758184486
640 640 g, t dbSNP:752397344
655 655 c, t dbSNP:142572513
667 667 a, c dbSNP:12292
675 675 c, t dbSNP:753826959
676 676 a, g, t dbSNP:376553875
694 694 c, t dbSNP:202147132
699 699 a, g dbSNP:750074607
701 701 a, g dbSNP:767113923
702 702 a, g dbSNP:761555796
706 706 a, g dbSNP:35447389
710 710 c, t dbSNP:770207315
727 727 a, g dbSNP:373374071
728 728 c, t dbSNP:777180494
733 733 a, g dbSNP:770978738
735 735 a, c dbSNP:747133568
741 741 a, c dbSNP:777942411
753 753 c, t dbSNP:772140491
756 756 c, t dbSNP:748419347
757 757 -, g dbSNP:35441215
757 757 c, t dbSNP:142637468
758 758 a, g dbSNP:150201810
761 761 g, t dbSNP:753810615
768 768 c, t dbSNP:780187035
775 775 c, t dbSNP:755653824
777 777 g, t dbSNP:749910886
778 778 c, g dbSNP:369150030
785 785 a, g dbSNP:143704611
786 786 a, c dbSNP:756800857
795 795 c, t dbSNP:751294000
799 799 g, t dbSNP:200070678
802 802 c, g dbSNP:765506968
806 806 a, g dbSNP:147918399
817 817 a, g dbSNP:777092611
835 835 c, t dbSNP:766834306
836 836 a, g dbSNP:373554215
841 841 c, t dbSNP:540635668
847 847 a, g dbSNP:14282
861 861 a, g dbSNP:748310171
867 867 a, c dbSNP:773783473
871 871 c, g, t dbSNP:768547475
875 875 a, g dbSNP:749133035
878 878 a, c, t dbSNP:756065296
879 879 a, g dbSNP:745831485
886 886 a, c, t dbSNP:756850765
889 889 a, g dbSNP:368195380
900 900 a, g dbSNP:763849216
903 903 a, g dbSNP:374747361
905 905 a, g dbSNP:758078998
927 927 a, g dbSNP:370831953
933 933 a, g dbSNP:766748201
949 949 c, t dbSNP:761107410
958 958 c, t dbSNP:773832373
965 965 -, aac dbSNP:764211158
967 967 c, t dbSNP:149890382
983 983 a, g dbSNP:184503723
985 985 c, t dbSNP:151171192
988 988 a, g dbSNP:769690555
991 991 c, t dbSNP:759416129
994 994 c, t dbSNP:776323409
996 996 c, t dbSNP:770855142
999 999 a, g dbSNP:199946928
1002 1002 c, t dbSNP:573326655
1003 1003 a, g dbSNP:568272529
1004 1004 c, t dbSNP:747781639
1017 1017 a, c dbSNP:778582930
1018 1018 a, g dbSNP:756400498
1021 1021 a, c dbSNP:750758098
1022 1022 c, g dbSNP:781431276
1040 1040 c, t dbSNP:398123542
1041 1041 a, g dbSNP:752116761
1048 1048 c, t dbSNP:764264501
1052 1052 a, g dbSNP:763174938
1054 1054 a, t dbSNP:752936749
1075 1075 c, t dbSNP:765517862
1083 1083 a, g dbSNP:533940631
1085 1085 g, t dbSNP:776431922
1086 1086 c, g dbSNP:770640062
1087 1087 a, g dbSNP:373664722
1088 1088 a, g dbSNP:773196469
1096 1096 c, t dbSNP:771544911
1097 1097 a, g dbSNP:369629631
1118 1118 a, t dbSNP:748965004
1119 1119 a, t dbSNP:775063184
1122 1122 c, t dbSNP:771296631
1135 1135 c, t dbSNP:371553398
1144 1144 c, t dbSNP:139930646
1145 1145 a, g dbSNP:758919353
1150 1150 c, t dbSNP:539328785
1153 1153 a, g dbSNP:779048381
1156 1156 a, g dbSNP:755074953
1174 1174 g, t dbSNP:754072239
1178 1178 a, c dbSNP:576613499
1181 1181 a, c dbSNP:756356313
1183 1183 c, t dbSNP:750167724
1192 1192 a, g dbSNP:199622147
1210 1210 c, t dbSNP:761669796
1216 1216 c, t dbSNP:536279337
1217 1217 a, g dbSNP:142542256
1220 1220 g, t dbSNP:762418853
1223 1223 a, g dbSNP:764846730
1239 1239 c, t dbSNP:759260191
1243 1243 c, t dbSNP:776487850
1245 1245 a, g dbSNP:148488868
1249 1249 a, g dbSNP:762128597
1267 1267 c, t dbSNP:138120227
1312 1312 g, t dbSNP:768998905
1336 1336 c, t dbSNP:749818060
1344 1344 c, t dbSNP:373857382
1354 1354 c, t dbSNP:374256179
1372 1372 a, g dbSNP:143083501
1382 1382 c, g dbSNP:774525572
1384 1384 a, g dbSNP:146846635
1387 1387 c, t dbSNP:556410164
1414 1414 c, t dbSNP:148177279
1415 1415 a, g dbSNP:763367920
1423 1423 c, g, t dbSNP:769842736
1426 1426 a, g dbSNP:745988780
1436 1436 c, g dbSNP:776713948
1441 1441 a, g dbSNP:770999988
1443 1443 g, t dbSNP:747258798
1447 1447 c, t dbSNP:777424675
1449 1449 a, g dbSNP:150399072
1463 1463 a, g dbSNP:754909186
1464 1464 c, t dbSNP:749245292
1476 1476 a, g dbSNP:779708532
1492 1492 c, t dbSNP:755710198
1493 1493 c, t dbSNP:121434491
1494 1494 a, g dbSNP:750022923
1496 1496 a, g dbSNP:576071059
1510 1510 c, t dbSNP:756890736
1531 1531 c, t dbSNP:753001473
1532 1532 c, t dbSNP:765589888
1533 1533 a, g dbSNP:372748426
1545 1545 a, g dbSNP:369049569
1556 1556 a, c dbSNP:777178356
1566 1566 a, g dbSNP:766343862
1567 1567 c, t dbSNP:760845391
1568 1568 a, c, g dbSNP:772284064
1578 1578 c, t dbSNP:762143333
1579 1579 a, g dbSNP:148823921
1580 1580 c, g dbSNP:774225644
1581 1581 a, g dbSNP:762613133
1587 1587 a, g dbSNP:779233876
1610 1610 c, g dbSNP:367901228
1615 1615 a, g dbSNP:754310502
1618 1618 c, t dbSNP:766866113
1620 1620 a, g dbSNP:146446706
1625 1625 a, c dbSNP:750455273
1650 1650 a, g dbSNP:767503998
1659 1659 c, t dbSNP:762126695
1662 1662 a, g, t dbSNP:775080680
1670 1670 a, g dbSNP:567386311
1671 1671 c, g dbSNP:139142559
1681 1681 a, g dbSNP:775180017
1712 1712 a, g dbSNP:142194873
1713 1713 g, t dbSNP:547851604
1734 1734 c, t dbSNP:780161368
1735 1735 c, t dbSNP:780748234
1740 1740 a, g dbSNP:776362721
1755 1755 a, g dbSNP:770484375
1756 1756 c, t dbSNP:534909303
1779 1779 a, g dbSNP:373990093
1780 1780 a, g dbSNP:777439665
1781 1781 c, g dbSNP:771649644
1784 1784 a, g dbSNP:747755796
1786 1786 a, g dbSNP:780466116
1796 1796 a, c dbSNP:533628546
1799 1799 g, t dbSNP:746366927
1802 1802 a, g dbSNP:781775380
1808 1808 a, g dbSNP:374643957
1810 1810 a, g, t dbSNP:147448993
1811 1811 c, t dbSNP:751641246
1814 1814 a, g dbSNP:139171666
1825 1825 a, g dbSNP:758641385
1828 1828 a, g dbSNP:149616551
1845 1845 c, t dbSNP:765057042
1846 1846 c, t dbSNP:759287964
1847 1847 a, g dbSNP:367718707
1872 1872 a, g dbSNP:766253306
1873 1873 c, t dbSNP:374690853
1878 1878 c, t dbSNP:772639652
1880 1880 c, g dbSNP:200959130
1890 1890 a, g dbSNP:201035117
1892 1892 a, c dbSNP:150488038
1893 1893 c, t dbSNP:770166720
1903 1903 g, t dbSNP:746975511
1923 1923 c, t dbSNP:746180588
1935 1935 c, t dbSNP:781708447
1938 1938 c, t dbSNP:112871166
1948 1948 -, t dbSNP:200536754
1951 1951 a, g dbSNP:199663914
2009 2009 a, t dbSNP:150344322
2015 2015 a, t dbSNP:541952588
2029 2029 a, g dbSNP:529125316
2042 2042 c, t dbSNP:113974409
2067 2067 g, t dbSNP:560047270
2088 2088 a, t dbSNP:540522685
2104 2104 c, t dbSNP:577486140
2110 2110 a, g dbSNP:1802574
2115 2115 c, t dbSNP:779068277
2124 2124 c, g dbSNP:755194729
2190 2190 a, c dbSNP:117386259
2191 2191 c, t dbSNP:373737354
2197 2197 a, g dbSNP:780676062
2199 2199 c, g dbSNP:181180919
2208 2208 c, t dbSNP:754725762
2222 2222 c, t dbSNP:535192173
2224 2224 c, t dbSNP:567064404
2225 2225 a, g dbSNP:553246404
2228 2228 a, g dbSNP:540160557
2277 2277 a, g dbSNP:571127990
2290 2290 c, t dbSNP:780507817
2292 2292 c, g dbSNP:780488845
2331 2331 c, t dbSNP:375379036
2337 2337 a, g dbSNP:571754691
2346 2346 c, g dbSNP:751018485
2387 2387 a, t dbSNP:765924429
2394 2394 -, t dbSNP:35704713
2403 2403 a, g dbSNP:1061326
2428 2428 a, g dbSNP:757690325
2444 2444 a, c dbSNP:530970873
2449 2449 c, t dbSNP:188698134
2475 2475 c, t dbSNP:56411268
2499 2499 c, t dbSNP:1063452
2516 2516 a, g dbSNP:548670062
2523 2523 c, t dbSNP:764390546
2524 2524 -, t dbSNP:55878434
2540 2540 g, t dbSNP:756425823
2591 2591 c, t dbSNP:186888998
2610 2610 c, t dbSNP:573892776
2629 2629 c, t dbSNP:138843899
2637 2637 c, t dbSNP:3791680
2638 2638 c, g dbSNP:181823941
2652 2652 c, t dbSNP:539540129
2742 2742 c, t dbSNP:146101049
2745 2745 a, c dbSNP:112572819
2872 2872 a, c dbSNP:762505852
2889 2889 a, g dbSNP:574934469
2904 2904 a, c dbSNP:77386452
2905 2905 a, g dbSNP:190519603
2935 2935 a, c dbSNP:185614902
2946 2946 c, g dbSNP:1802575
2953 2953 a, g dbSNP:568837686
2968 2968 c, t dbSNP:540075808
3004 3004 c, t dbSNP:142276628
3005 3005 a, g dbSNP:112334283
3007 3007 a, g dbSNP:147551451
3019 3019 a, g dbSNP:568443337
3035 3035 c, t dbSNP:548260378
3053 3053 a, g dbSNP:192552476

Target ORF information:

RefSeq Version NM_001039349
Organism Homo sapiens (human)
Definition Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039349

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.