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EFEMP1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EFEMP1
Entrez Gene ID 2202
Full Name EGF containing fibulin-like extracellular matrix protein 1
Synonyms DHRD, DRAD, FBLN3, FBNL, FIBL-3, MLVT, MTLV, S1-5
General protein information
Preferred Names
EGF-containing fibulin-like extracellular matrix protein 1
Names
EGF-containing fibulin-like extracellular matrix protein 1
fibulin-3
extracellular protein S1-5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p16

Summary This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

601548

Disorder Html: Doyne honeycomb degeneration of retina, 126600 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005264205 XP_005264262 EGF-containing fibulin-like extracellular matrix protein 1 isoform X1
NM_001039348 NP_001034437 EGF-containing fibulin-like extracellular matrix protein 1 precursor
NM_001039349 NP_001034438 EGF-containing fibulin-like extracellular matrix protein 1 precursor



Homo sapiens (human) EFEMP1 NP_001034438.1
Canis lupus familiaris (dog) EFEMP1 XP_005626175.1
Bos taurus (cattle) EFEMP1 NP_001075186.1
Mus musculus (house mouse) Efemp1 NP_666127.2
Rattus norvegicus (Norway rat) Efemp1 NP_001012039.1
Gallus gallus (chicken) EFEMP1 XP_426097.3
Danio rerio (zebrafish) LOC100332567 XP_002664117.2
Xenopus (Silurana) tropicalis (western clawed frog) efemp1 XP_002936872.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EFEMP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFEMP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35325 XM_005264205 PREDICTED: Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
OHu22367
NM_001039348 Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22367
NM_001039349 Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu35325
Clone ID Related Accession (Same CDS sequence) XM_005264205
Accession Version XM_005264205.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product EGF-containing fibulin-like extracellular matrix protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802779. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCAACGT GTCCTCTCTG GCGTGGGGTC CCCGCGAGTC TGGGAAACGA GGAGCTGGCA 
AGGGAGGGCA AGCGGGGGCG GCAAAGAGGG CCCCTGGAGC AGGGGGCGCG GACCTCGCTG
CGCTGCGCGC TCTACCGCCG GGCTCGCAAC GCTGGGCTCA GCGCTCGCGC CTCCCTCAGC
TCTCTCCTCC GCCCCCCTTC GCCCTCCCCC TTTCCCTCCC TTTCTCCTCC TCCTCCTGCC
GCCGCGGCCG CTGCCGGACT TCGCCAGATC AGACCCACGG GGCTGCCCTC CCCTGCGCAC
TCCCCTCGCT GCCCGGGCCC GGAGCGCAGC GCGGCCGCAC AGGTATTTTT GCTGTGCTGT
GCAAGGAACT CTGCTAGCTC AAGATTCACA ATGTTGAAAG CCCTTTTCCT AACTATGCTG
ACTCTGGCGC TGGTCAAGTC ACAGGACACC GAAGAAACCA TCACGTACAC GCAATGCACT
GACGGATATG AGTGGGATCC TGTGAGACAG CAATGCAAAG ATATTGATGA ATGTGACATT
GTCCCAGACG CTTGTAAAGG TGGAATGAAG TGTGTCAACC ACTATGGAGG ATACCTCTGC
CTTCCGAAAA CAGCCCAGAT TATTGTCAAT AATGAACAGC CTCAGCAGGA AACACAACCA
GCAGAAGGAA CCTCAGGGGC AACCACCGGG GTTGTAGCTG CCAGCAGCAT GGCAACCAGT
GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC AGTGCTGCTG CAGTCGCAGG CCCTGAAATG
CAGACTGGCC GAAATAACTT TGTCATCCGG CGGAACCCAG CTGACCCTCA GCGCATTCCC
TCCAACCCTT CCCACCGTAT CCAGTGTGCA GCAGGCTACG AGCAAAGTGA ACACAACGTG
TGCCAAGACA TAGACGAGTG CACTGCAGGG ACGCACAACT GTAGAGCAGA CCAAGTGTGC
ATCAATTTAC GGGGATCCTT TGCATGTCAG TGCCCTCCTG GATATCAGAA GCGAGGGGAG
CAGTGCGTAG ACATTGATGA ATGCAGAACC TCAAGCTACC TGTGTCAATA TCAATGTGTC
AATGAACCTG GGAAATTCTC ATGTATGTGC CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA
ACATGTCAAG ATATAAATGA GTGTGAGACC ACAAATGAAT GCCGGGAGGA TGAAATGTGT
TGGAATTATC ATGGCGGCTT CCGTTGTTAT CCACGAAATC CTTGTCAAGA TCCCTACATT
CTAACACCAG AGAACCGATG TGTTTGCCCA GTCTCAAATG CCATGTGCCG AGAACTGCCC
CAGTCAATAG TCTACAAATA CATGAGCATC CGATCTGATA GGTCTGTGCC ATCAGACATC
TTCCAGATAC AGGCCACAAC TATTTATGCC AACACCATCA ATACTTTTCG GATTAAATCT
GGAAATGAAA ATGGAGAGTT CTACCTACGA CAAACAAGTC CTGTAAGTGC AATGCTTGTG
CTCGTGAAGT CATTATCAGG ACCAAGAGAA CATATCGTGG ACCTGGAGAT GCTGACAGTC
AGCAGTATAG GGACCTTCCG CACAAGCTCT GTGTTAAGAT TGACAATAAT AGTGGGGCCA
TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264262.1
CDS112..1743
Misc Feature(1)631..>714(+)
Misc Feature(2)1018..1140(+)
Misc Feature(3)1018..1077(+)
Misc Feature(4)1141..1260(+)
Misc Feature(5)1141..1194(+)
Misc Feature(6)1261..1377(+)
Misc Feature(7)1261..1317(+)
Translation

Target ORF information:

RefSeq Version XM_005264205
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264205

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCAACGT GTCCTCTCTG GCGTGGGGTC CCCGCGAGTC TGGGAAACGA GGAGCTGGCA 
AGGGAGGGCA AGCGGGGGCG GCAAAGAGGG CCCCTGGAGC AGGGGGCGCG GACCTCGCTG
CGCTGCGCGC TCTACCGCCG GGCTCGCAAC GCTGGGCTCA GCGCTCGCGC CTCCCTCAGC
TCTCTCCTCC GCCCCCCTTC GCCCTCCCCC TTTCCCTCCC TTTCTCCTCC TCCTCCTGCC
GCCGCGGCCG CTGCCGGACT TCGCCAGATC AGACCCACGG GGCTGCCCTC CCCTGCGCAC
TCCCCTCGCT GCCCGGGCCC GGAGCGCAGC GCGGCCGCAC AGGTATTTTT GCTGTGCTGT
GCAAGGAACT CTGCTAGCTC AAGATTCACA ATGTTGAAAG CCCTTTTCCT AACTATGCTG
ACTCTGGCGC TGGTCAAGTC ACAGGACACC GAAGAAACCA TCACGTACAC GCAATGCACT
GACGGATATG AGTGGGATCC TGTGAGACAG CAATGCAAAG ATATTGATGA ATGTGACATT
GTCCCAGACG CTTGTAAAGG TGGAATGAAG TGTGTCAACC ACTATGGAGG ATACCTCTGC
CTTCCGAAAA CAGCCCAGAT TATTGTCAAT AATGAACAGC CTCAGCAGGA AACACAACCA
GCAGAAGGAA CCTCAGGGGC AACCACCGGG GTTGTAGCTG CCAGCAGCAT GGCAACCAGT
GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC AGTGCTGCTG CAGTCGCAGG CCCTGAAATG
CAGACTGGCC GAAATAACTT TGTCATCCGG CGGAACCCAG CTGACCCTCA GCGCATTCCC
TCCAACCCTT CCCACCGTAT CCAGTGTGCA GCAGGCTACG AGCAAAGTGA ACACAACGTG
TGCCAAGACA TAGACGAGTG CACTGCAGGG ACGCACAACT GTAGAGCAGA CCAAGTGTGC
ATCAATTTAC GGGGATCCTT TGCATGTCAG TGCCCTCCTG GATATCAGAA GCGAGGGGAG
CAGTGCGTAG ACATTGATGA ATGCAGAACC TCAAGCTACC TGTGTCAATA TCAATGTGTC
AATGAACCTG GGAAATTCTC ATGTATGTGC CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA
ACATGTCAAG ATATAAATGA GTGTGAGACC ACAAATGAAT GCCGGGAGGA TGAAATGTGT
TGGAATTATC ATGGCGGCTT CCGTTGTTAT CCACGAAATC CTTGTCAAGA TCCCTACATT
CTAACACCAG AGAACCGATG TGTTTGCCCA GTCTCAAATG CCATGTGCCG AGAACTGCCC
CAGTCAATAG TCTACAAATA CATGAGCATC CGATCTGATA GGTCTGTGCC ATCAGACATC
TTCCAGATAC AGGCCACAAC TATTTATGCC AACACCATCA ATACTTTTCG GATTAAATCT
GGAAATGAAA ATGGAGAGTT CTACCTACGA CAAACAAGTC CTGTAAGTGC AATGCTTGTG
CTCGTGAAGT CATTATCAGG ACCAAGAGAA CATATCGTGG ACCTGGAGAT GCTGACAGTC
AGCAGTATAG GGACCTTCCG CACAAGCTCT GTGTTAAGAT TGACAATAAT AGTGGGGCCA
TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22367
Clone ID Related Accession (Same CDS sequence) NM_001039348 , NM_001039349
Accession Version NM_001039348.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product EGF-containing fibulin-like extracellular matrix protein 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA264704.1, AK312639.1, AK290599.1, AC010895.8 and U03877.1. This sequence is a reference standard in the RefSeqGene project. On Jan 27, 2011 this sequence version replaced gi:86788014. Transcript Variant: This variant (2) represents the longer transcript. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12242346) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC098561.1, AK290599.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001034437.1
CDS502..1983
Misc Feature(1)19..21(+)
Misc Feature(2)1018..1140(+)
Misc Feature(3)1018..1077(+)
Misc Feature(4)1141..1236(+)
Misc Feature(5)1141..1191(+)
Misc Feature(6)1255..1374(+)
Misc Feature(7)1276..1980(+)
Misc Feature(8)1321..1392(+)
Misc Feature(9)1375..1473(+)
Misc Feature(10)1501..>1578(+)
Exon (1)1..453
Gene:EFEMP1
Gene Synonym:
Exon (2)454..494
Gene:EFEMP1
Gene Synonym:
Exon (3)495..582
Gene:EFEMP1
Gene Synonym:
Exon (4)583..631
Gene:EFEMP1
Gene Synonym:
Exon (5)632..1018
Gene:EFEMP1
Gene Synonym:
Exon (6)1019..1141
Gene:EFEMP1
Gene Synonym:
Exon (7)1142..1261
Gene:EFEMP1
Gene Synonym:
Exon (8)1262..1381
Gene:EFEMP1
Gene Synonym:
Exon (9)1382..1501
Gene:EFEMP1
Gene Synonym:
Exon (10)1502..1625
Gene:EFEMP1
Gene Synonym:
Exon (11)1626..1821
Gene:EFEMP1
Gene Synonym:
Exon (12)1822..3094
Gene:EFEMP1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001039348
Organism Homo sapiens (human)
Definition Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039348

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22367
Clone ID Related Accession (Same CDS sequence) NM_001039348 , NM_001039349
Accession Version NM_001039349.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product EGF-containing fibulin-like extracellular matrix protein 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA264704.1, BC014410.1, AC010895.8 and U03877.1. On Jan 27, 2011 this sequence version replaced gi:86788131. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 2. Variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293058.1, BC014410.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001034438.1
CDS461..1942
Misc Feature(1)65..67(+)
Misc Feature(2)977..1099(+)
Misc Feature(3)977..1036(+)
Misc Feature(4)1100..1195(+)
Misc Feature(5)1100..1150(+)
Misc Feature(6)1214..1333(+)
Misc Feature(7)1235..1939(+)
Misc Feature(8)1280..1351(+)
Misc Feature(9)1334..1432(+)
Misc Feature(10)1460..>1537(+)
Exon (1)1..453
Gene:EFEMP1
Gene Synonym:
Exon (2)454..541
Gene:EFEMP1
Gene Synonym:
Exon (3)542..590
Gene:EFEMP1
Gene Synonym:
Exon (4)591..977
Gene:EFEMP1
Gene Synonym:
Exon (5)978..1100
Gene:EFEMP1
Gene Synonym:
Exon (6)1101..1220
Gene:EFEMP1
Gene Synonym:
Exon (7)1221..1340
Gene:EFEMP1
Gene Synonym:
Exon (8)1341..1460
Gene:EFEMP1
Gene Synonym:
Exon (9)1461..1584
Gene:EFEMP1
Gene Synonym:
Exon (10)1585..1780
Gene:EFEMP1
Gene Synonym:
Exon (11)1781..3053
Gene:EFEMP1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001039349
Organism Homo sapiens (human)
Definition Homo sapiens EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039349

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGAAAG CCCTTTTCCT AACTATGCTG ACTCTGGCGC TGGTCAAGTC ACAGGACACC 
GAAGAAACCA TCACGTACAC GCAATGCACT GACGGATATG AGTGGGATCC TGTGAGACAG
CAATGCAAAG ATATTGATGA ATGTGACATT GTCCCAGACG CTTGTAAAGG TGGAATGAAG
TGTGTCAACC ACTATGGAGG ATACCTCTGC CTTCCGAAAA CAGCCCAGAT TATTGTCAAT
AATGAACAGC CTCAGCAGGA AACACAACCA GCAGAAGGAA CCTCAGGGGC AACCACCGGG
GTTGTAGCTG CCAGCAGCAT GGCAACCAGT GGAGTGTTGC CCGGGGGTGG TTTTGTGGCC
AGTGCTGCTG CAGTCGCAGG CCCTGAAATG CAGACTGGCC GAAATAACTT TGTCATCCGG
CGGAACCCAG CTGACCCTCA GCGCATTCCC TCCAACCCTT CCCACCGTAT CCAGTGTGCA
GCAGGCTACG AGCAAAGTGA ACACAACGTG TGCCAAGACA TAGACGAGTG CACTGCAGGG
ACGCACAACT GTAGAGCAGA CCAAGTGTGC ATCAATTTAC GGGGATCCTT TGCATGTCAG
TGCCCTCCTG GATATCAGAA GCGAGGGGAG CAGTGCGTAG ACATAGATGA ATGTACCATC
CCTCCATATT GCCACCAAAG ATGCGTGAAT ACACCAGGCT CATTTTATTG CCAGTGCAGT
CCTGGGTTTC AATTGGCAGC AAACAACTAT ACCTGCGTAG ATATAAATGA ATGTGATGCC
AGCAATCAAT GTGCTCAGCA GTGCTACAAC ATTCTTGGTT CATTCATCTG TCAGTGCAAT
CAAGGATATG AGCTAAGCAG TGACAGGCTC AACTGTGAAG ACATTGATGA ATGCAGAACC
TCAAGCTACC TGTGTCAATA TCAATGTGTC AATGAACCTG GGAAATTCTC ATGTATGTGC
CCCCAGGGAT ACCAAGTGGT GAGAAGTAGA ACATGTCAAG ATATAAATGA GTGTGAGACC
ACAAATGAAT GCCGGGAGGA TGAAATGTGT TGGAATTATC ATGGCGGCTT CCGTTGTTAT
CCACGAAATC CTTGTCAAGA TCCCTACATT CTAACACCAG AGAACCGATG TGTTTGCCCA
GTCTCAAATG CCATGTGCCG AGAACTGCCC CAGTCAATAG TCTACAAATA CATGAGCATC
CGATCTGATA GGTCTGTGCC ATCAGACATC TTCCAGATAC AGGCCACAAC TATTTATGCC
AACACCATCA ATACTTTTCG GATTAAATCT GGAAATGAAA ATGGAGAGTT CTACCTACGA
CAAACAAGTC CTGTAAGTGC AATGCTTGTG CTCGTGAAGT CATTATCAGG ACCAAGAGAA
CATATCGTGG ACCTGGAGAT GCTGACAGTC AGCAGTATAG GGACCTTCCG CACAAGCTCT
GTGTTAAGAT TGACAATAAT AGTGGGGCCA TTTTCATTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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