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FGB cDNA ORF clone, Homo sapiens (human)

Gene Symbol FGB
Entrez Gene ID 2244
Full Name fibrinogen beta chain
Synonyms HEL-S-78p
General protein information
Preferred Names
fibrinogen beta chain
Names
fibrinogen beta chain
fibrinogen, B beta polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q28

Summary The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]. lac of sum
Disorder MIM:

134830

Disorder Html: Dysfibrinogenemia, beta type (3); Afibrinogenemia, congenital,





The following FGB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu28464 NM_005141 Homo sapiens fibrinogen beta chain (FGB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$286.30
$409.00
OHu28452 NM_001184741 Homo sapiens fibrinogen beta chain (FGB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $359.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu28464
Accession Version NM_005141.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1476bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibrinogen beta chain isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312972.1, J00129.1 and AC107385.4. This sequence is a reference standard in the RefSeqGene project. On May 15, 2010 this sequence version replaced gi:188595719. Summary: The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312972.1, EU668333.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAAAAGGA TGGTTTCTTG GAGCTTCCAC AAACTTAAAA CCATGAAACA TCTATTATTG 
CTACTATTGT GTGTTTTTCT AGTTAAGTCC CAAGGTGTCA ACGACAATGA GGAGGGTTTC
TTCAGTGCCC GTGGTCATCG ACCCCTTGAC AAGAAGAGAG AAGAGGCTCC CAGCCTGAGG
CCTGCCCCAC CGCCCATCAG TGGAGGTGGC TATCGGGCTC GTCCAGCCAA AGCAGCTGCC
ACTCAAAAGA AAGTAGAAAG AAAAGCCCCT GATGCTGGAG GCTGTCTTCA CGCTGACCCA
GACCTGGGGG TGTTGTGTCC TACAGGATGT CAGTTGCAAG AGGCTTTGCT ACAACAGGAA
AGGCCAATCA GAAATAGTGT TGATGAGTTA AATAACAATG TGGAAGCTGT TTCCCAGACC
TCCTCTTCTT CCTTTCAGTA CATGTATTTG CTGAAAGACC TGTGGCAAAA GAGGCAGAAG
CAAGTAAAAG ATAATGAAAA TGTAGTCAAT GAGTACTCCT CAGAACTGGA AAAGCACCAA
TTATATATAG ATGAGACTGT GAATAGCAAT ATCCCAACTA ACCTTCGTGT GCTTCGTTCA
ATCCTGGAAA ACCTGAGAAG CAAAATACAA AAGTTAGAAT CTGATGTCTC AGCTCAAATG
GAATATTGTC GCACCCCATG CACTGTCAGT TGCAATATTC CTGTGGTGTC TGGCAAAGAA
TGTGAGGAAA TTATCAGGAA AGGAGGTGAA ACATCTGAAA TGTATCTCAT TCAACCTGAC
AGTTCTGTCA AACCGTATAG AGTATACTGT GACATGAATA CAGAAAATGG AGGATGGACA
GTGATTCAGA ACCGTCAAGA CGGTAGTGTT GACTTTGGCA GGAAATGGGA TCCATATAAA
CAGGGATTTG GAAATGTTGC AACCAACACA GATGGGAAGA ATTACTGTGG CCTACCAGGT
GAATATTGGC TTGGAAATGA TAAAATTAGC CAGCTTACCA GGATGGGACC CACAGAACTT
TTGATAGAAA TGGAGGACTG GAAAGGAGAC AAAGTAAAGG CTCACTATGG AGGATTCACT
GTACAGAATG AAGCCAACAA ATACCAGATC TCAGTGAACA AATACAGAGG AACAGCCGGT
AATGCCCTCA TGGATGGAGC ATCTCAGCTG ATGGGAGAAA ACAGGACCAT GACCATTCAC
AACGGCATGT TCTTCAGCAC GTATGACAGA GACAATGACG GCTGGTTAAC ATCAGATCCC
AGAAAACAGT GTTCTAAAGA AGACGGTGGT GGATGGTGGT ATAATAGATG TCATGCAGCC
AATCCAAACG GCAGATACTA CTGGGGTGGA CAGTACACCT GGGACATGGC AAAGCATGGC
ACAGATGATG GTGTAGTATG GATGAATTGG AAGGGGTCAT GGTACTCAAT GAGGAAGATG
AGTATGAAGA TCAGGCCCTT CTTCCCACAG CAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005132.2
CDS40..1515
Misc Feature(1)31..33(+)
Misc Feature(2)130..132(+)
Misc Feature(3)169..174(+)
Misc Feature(4)172..180(+)
Misc Feature(5)307..744(+)
Misc Feature(6)493..498(+)
Misc Feature(7)517..522(+)
Misc Feature(8)526..531(+)
Misc Feature(9)751..1500(+)
Misc Feature(10)1144..1146(+)
Misc Feature(11)1270..1284(+)
Misc Feature(12)1309..1353(+)
Exon (1)1..153
Gene:FGB
Gene Synonym:
Exon (2)154..345
Gene:FGB
Gene Synonym:
Exon (3)346..529
Gene:FGB
Gene Synonym:
Exon (4)530..757
Gene:FGB
Gene Synonym:
Exon (5)758..871
Gene:FGB
Gene Synonym:
Exon (6)872..997
Gene:FGB
Gene Synonym:
Exon (7)998..1283
Gene:FGB
Gene Synonym:
Exon (8)1284..3628
Gene:FGB
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, g dbSNP:564463049
10 10 c, t dbSNP:768398827
12 12 c, g dbSNP:776370184
15 15 c, t dbSNP:761398967
25 25 c, g dbSNP:576348167
28 28 a, c dbSNP:375151084
31 31 a, g, t dbSNP:113961753
34 34 -, gt dbSNP:757727864
34 34 a, g dbSNP:36203394
39 39 c, t dbSNP:543846564
40 40 a, g dbSNP:751892977
43 43 a, g dbSNP:6053
49 49 a, t dbSNP:767891657
56 56 c, g dbSNP:752925190
70 70 a, g dbSNP:756304323
73 73 c, g dbSNP:777895153
75 75 a, t dbSNP:376146726
105 105 a, c dbSNP:758377428
118 118 c, t dbSNP:11099960
123 123 a, t dbSNP:375340782
125 125 a, g dbSNP:746963310
136 136 a, g dbSNP:562142566
141 141 c, t dbSNP:780849917
142 142 a, g dbSNP:747783086
143 143 a, g dbSNP:774271682
148 148 a, g dbSNP:769395119
162 162 c, t dbSNP:770388453
164 164 a, g dbSNP:773855571
169 169 c, t dbSNP:121909616
170 170 a, g dbSNP:746361316
172 172 g, t dbSNP:121909618
178 178 c, t dbSNP:121909625
179 179 a, g dbSNP:775891227
180 180 a, g dbSNP:6059
187 187 a, g dbSNP:760992086
194 194 a, g dbSNP:764538840
196 196 a, g dbSNP:776731258
201 201 a, g dbSNP:762126974
205 205 g, t dbSNP:765345762
214 214 c, t dbSNP:34851875
227 227 c, t dbSNP:750593479
230 230 c, t dbSNP:141013999
231 231 a, g dbSNP:761354866
239 239 c, g dbSNP:371842822
253 253 c, t dbSNP:755819016
254 254 a, g dbSNP:144972538
255 255 a, g dbSNP:553042170
259 259 c, t dbSNP:121909619
260 260 a, g dbSNP:571624045
262 262 c, t dbSNP:756917559
266 266 a, c dbSNP:778390845
273 273 a, g dbSNP:745422213
281 281 c, g dbSNP:764854641
287 287 a, g dbSNP:772608486
293 293 c, t dbSNP:775809959
294 294 a, g dbSNP:747581058
296 296 a, g dbSNP:11557357
298 298 a, g dbSNP:768833298
300 300 a, t dbSNP:777144899
304 304 c, g dbSNP:762110358
311 311 a, t dbSNP:765627047
321 321 c, t dbSNP:773398230
330 330 c, t dbSNP:6060
331 331 a, g dbSNP:121909620
337 337 a, c, t dbSNP:2227434
346 346 a, g dbSNP:774502903
348 348 c, g dbSNP:145524939
349 349 a, g, t dbSNP:148864798
351 351 a, g dbSNP:761544987
357 357 c, t dbSNP:6055
366 366 a, t dbSNP:750008000
371 371 a, g dbSNP:758080375
374 374 c, t dbSNP:779413540
382 382 c, g dbSNP:751143215
390 390 a, g dbSNP:199768390
396 396 a, g dbSNP:755412339
402 402 a, g dbSNP:570183893
406 406 a, g dbSNP:148045912
409 409 a, g dbSNP:746024226
410 410 a, g dbSNP:748690444
421 421 a, g dbSNP:770246669
430 430 a, g dbSNP:142641543
436 436 a, g dbSNP:749412950
440 440 c, t dbSNP:771243649
441 441 c, g dbSNP:774263206
446 446 c, g dbSNP:759710385
479 479 a, c dbSNP:772160258
485 485 a, g dbSNP:776510812
494 494 a, g dbSNP:761740955
499 499 c, t dbSNP:764952511
515 515 a, t dbSNP:199596249
516 516 a, g dbSNP:762504717
518 518 a, g dbSNP:113610714
522 522 a, g dbSNP:766099099
523 523 g, t dbSNP:751016082
524 524 c, t dbSNP:754492004
540 540 c, t dbSNP:767079997
547 547 a, c dbSNP:2227409
549 549 a, t dbSNP:149963684
550 550 c, g dbSNP:764709626
552 552 a, g dbSNP:754323874
561 561 a, g dbSNP:757599938
564 564 a, c dbSNP:779081524
570 570 a, g dbSNP:201525782
573 573 c, g, t dbSNP:201909029
574 574 c, t dbSNP:543323660
579 579 a, c, g dbSNP:747127658
582 582 a, g dbSNP:777720766
586 586 a, g dbSNP:749217408
591 591 c, t dbSNP:770695888
598 598 a, g dbSNP:377741367
603 603 c, t dbSNP:759250713
606 606 c, t dbSNP:6056
613 613 c, g dbSNP:1043726
616 616 a, g dbSNP:775110705
618 618 g, t dbSNP:141978904
621 621 c, t dbSNP:780364193
625 625 c, t dbSNP:121909623
626 626 a, g dbSNP:530036524
632 632 c, t dbSNP:754302976
634 634 c, t dbSNP:747314723
635 635 a, g dbSNP:548206997
642 642 c, t dbSNP:150838423
644 644 a, t dbSNP:121909624
645 645 a, g dbSNP:1043727
663 663 a, c dbSNP:758583316
664 664 a, c dbSNP:139311055
665 665 c, t dbSNP:538708636
670 670 a, g dbSNP:374363120
673 673 c, t dbSNP:751805410
687 687 c, g dbSNP:755049679
689 689 c, t dbSNP:781138401
693 693 c, g, t dbSNP:749194615
695 695 a, g dbSNP:778766319
696 696 a, g dbSNP:745699048
709 709 c, t dbSNP:772014512
710 710 a, g dbSNP:775243595
721 721 a, g dbSNP:149599496
722 722 c, t dbSNP:564278470
727 727 a, c, t dbSNP:776059376
732 732 c, t dbSNP:776862282
737 737 g, t dbSNP:765701687
739 739 c, t dbSNP:750761651
745 745 g, t dbSNP:763257449
746 746 c, t dbSNP:144336893
785 785 g, t dbSNP:369272403
788 788 a, g dbSNP:759800033
790 790 a, g dbSNP:541789688
791 791 c, t dbSNP:776988939
796 796 c, g dbSNP:756100115
801 801 a, g dbSNP:201536638
803 803 a, g dbSNP:750327950
809 809 c, t dbSNP:758467916
819 819 c, g dbSNP:779988201
825 825 c, t dbSNP:746815835
831 831 a, g dbSNP:199550432
833 833 c, t dbSNP:6054
834 834 a, g dbSNP:144912407
836 836 a, g dbSNP:370703973
845 845 a, c dbSNP:772727358
846 846 c, t dbSNP:749785846
855 855 g, t dbSNP:147961877
867 867 c, t dbSNP:771496034
881 881 c, t dbSNP:771406104
882 882 g, t dbSNP:6058
883 883 a, t dbSNP:746420120
893 893 a, g dbSNP:748781687
894 894 c, t dbSNP:772265368
895 895 c, t dbSNP:775981072
900 900 c, t dbSNP:146831181
901 901 a, g dbSNP:769036186
906 906 c, t dbSNP:773739999
918 918 c, t dbSNP:761932805
928 928 a, g dbSNP:759172132
930 930 a, t dbSNP:199592944
931 931 c, t dbSNP:765435305
933 933 a, g dbSNP:200755352
938 938 a, g dbSNP:759534465
941 941 a, t dbSNP:767207616
945 945 a, t dbSNP:142064127
955 955 a, g dbSNP:572344189
959 959 c, t dbSNP:777451745
962 962 a, c dbSNP:753513033
964 964 a, t dbSNP:181245987
965 965 a, g dbSNP:778498115
969 969 a, g dbSNP:746345704
972 972 g, t dbSNP:151153332
974 974 a, g dbSNP:780249202
983 983 a, c dbSNP:564407258
990 990 c, t dbSNP:140191144
995 995 c, t dbSNP:374463429
1002 1002 a, g dbSNP:781702393
1012 1012 g, t dbSNP:748371656
1014 1014 a, t dbSNP:770123797
1018 1018 g, t dbSNP:368867072
1036 1036 a, g dbSNP:749434722
1040 1040 a, g dbSNP:770938745
1064 1064 c, t dbSNP:766420576
1065 1065 a, g, t dbSNP:751610009
1066 1066 a, g dbSNP:763862526
1070 1070 c, t dbSNP:150330219
1074 1074 a, g dbSNP:755184215
1084 1084 a, g dbSNP:761339656
1085 1085 c, g dbSNP:764989450
1089 1089 c, t dbSNP:547765642
1090 1090 -, aaagt dbSNP:761057382
1097 1097 a, g dbSNP:763287118
1098 1098 c, g dbSNP:765808222
1104 1104 -, caaaaa, caaaaataaaattt dbSNP:764656753
1106 1106 -, aaattt dbSNP:762214685
1108 1108 a, g dbSNP:752051727
1111 1111 a, g dbSNP:755602626
1118 1118 c, t dbSNP:781689806
1120 1120 g, t dbSNP:748566219
1131 1131 a, g dbSNP:753038061
1132 1132 a, g dbSNP:121909617
1149 1149 c, g, t dbSNP:756271964
1152 1152 a, g dbSNP:6057
1156 1156 a, t dbSNP:141025407
1160 1160 a, c dbSNP:774429340
1164 1164 c, t dbSNP:4681
1167 1167 a, g dbSNP:749738413
1171 1171 a, c dbSNP:776386995
1176 1176 c, t dbSNP:371254124
1177 1177 a, g dbSNP:764970021
1179 1179 c, t dbSNP:771357492
1182 1182 c, t dbSNP:778439014
1186 1186 c, g dbSNP:762523152
1187 1187 g, t dbSNP:121909621
1188 1188 c, t dbSNP:549444448
1191 1191 g, t dbSNP:765976401
1201 1201 a, t dbSNP:751184886
1211 1211 c, t dbSNP:138760475
1214 1214 c, g dbSNP:768192369
1218 1218 a, g dbSNP:753167823
1222 1222 a, g dbSNP:569380660
1224 1224 -, gaccat dbSNP:765705017
1228 1228 a, g dbSNP:200112613
1229 1229 c, t dbSNP:62637604
1230 1230 a, g dbSNP:140466984
1238 1238 a, c dbSNP:754035448
1242 1242 a, c, t dbSNP:771728339
1243 1243 a, g dbSNP:200792588
1260 1260 a, g dbSNP:143651398
1266 1266 c, t dbSNP:768641694
1273 1273 a, t dbSNP:776578431
1278 1278 c, t dbSNP:148950857
1279 1279 a, g dbSNP:141881199
1288 1288 a, g dbSNP:530461300
1291 1291 a, t dbSNP:749089748
1292 1292 c, t dbSNP:770876148
1296 1296 a, t dbSNP:774106857
1297 1297 c, g dbSNP:759268165
1322 1322 a, g dbSNP:553753567
1323 1323 c, t dbSNP:771651132
1324 1324 a, g dbSNP:199833798
1326 1326 c, t dbSNP:369472452
1328 1328 a, g dbSNP:121909622
1331 1331 g, t dbSNP:779328318
1344 1344 c, t dbSNP:745365985
1348 1348 c, t dbSNP:764555923
1350 1350 a, t dbSNP:758044306
1356 1356 a, t dbSNP:548663056
1364 1364 c, t dbSNP:762125715
1368 1368 c, t dbSNP:150650205
1377 1377 c, t dbSNP:528172101
1380 1380 c, t dbSNP:754698865
1382 1382 g, t dbSNP:199846554
1384 1384 c, g dbSNP:758674997
1393 1393 c, t dbSNP:766473963
1395 1395 c, t dbSNP:751634292
1405 1405 a, g dbSNP:756060132
1408 1408 a, g dbSNP:777526125
1415 1415 a, g dbSNP:749273737
1417 1417 a, g dbSNP:200163097
1427 1427 a, g dbSNP:112136436
1431 1431 a, t dbSNP:779851430
1445 1445 a, g dbSNP:202102031
1448 1448 a, g dbSNP:374178110
1449 1449 a, g dbSNP:113985604
1455 1455 g, t dbSNP:745503243
1458 1458 a, t dbSNP:571421762
1464 1464 c, t dbSNP:139212482
1469 1469 c, t dbSNP:558143752
1470 1470 a, g dbSNP:201307744
1472 1472 a, g dbSNP:4220
1479 1479 a, g dbSNP:762320286
1483 1483 a, g dbSNP:765571602
1486 1486 a, c dbSNP:773632388
1489 1489 a, c dbSNP:763133457
1497 1497 c, t dbSNP:766560796
1503 1503 c, t dbSNP:751830311
1525 1525 c, g, t dbSNP:376861907
1526 1526 a, g dbSNP:747422456
1530 1530 a, c, g dbSNP:375561591
1546 1546 a, g dbSNP:745703563
1551 1551 -, aca dbSNP:758707026
1551 1551 a, g dbSNP:758231685
1557 1557 a, t dbSNP:779529144
1563 1563 c, t dbSNP:746673921
1564 1564 a, g dbSNP:370076235
1748 1748 g, t dbSNP:539731011
1759 1759 g, t dbSNP:574115053
1778 1778 a, g dbSNP:141024945
1807 1807 g, t dbSNP:776523403
1846 1846 a, c dbSNP:559920720
1864 1864 a, g dbSNP:188621081
1877 1877 c, t dbSNP:747930205
1878 1878 a, g dbSNP:545671394
1885 1885 c, t dbSNP:150273093
1888 1888 a, g dbSNP:2227420
1924 1924 c, t dbSNP:192770089
1937 1937 a, c dbSNP:2227421
1944 1944 g, t dbSNP:759655332
1958 1958 a, g dbSNP:2227422
1962 1962 c, t dbSNP:2227423
1965 1965 a, t dbSNP:183181144
1973 1973 a, c dbSNP:543668072
1987 1987 a, g dbSNP:538497057
2003 2003 a, c dbSNP:374456153
2011 2011 a, g dbSNP:557290052
2023 2023 g, t dbSNP:139295173
2090 2090 c, t dbSNP:537427494
2106 2106 c, t dbSNP:760839598
2107 2107 a, c, g dbSNP:376470048
2117 2117 c, t dbSNP:555815994
2134 2134 c, t dbSNP:2227439
2139 2139 c, t dbSNP:754198978
2140 2140 a, g dbSNP:757615748
2168 2168 a, c dbSNP:372503293
2187 2187 a, g dbSNP:541521264
2201 2201 a, g dbSNP:375313300
2227 2227 a, g dbSNP:765764692
2333 2333 -, a dbSNP:368605323
2334 2334 -, a dbSNP:555952609
2349 2349 -, a dbSNP:57313183
2350 2350 -, g dbSNP:768727295
2361 2361 g, t dbSNP:541828079
2433 2433 c, g dbSNP:2227424
2463 2463 a, g dbSNP:372231882
2525 2525 c, t dbSNP:527937994
2542 2542 c, t dbSNP:563622201
2556 2556 a, t dbSNP:768949107
2585 2585 c, g dbSNP:2227425
2593 2593 c, t dbSNP:758981171
2594 2594 a, c dbSNP:187861993
2602 2602 c, t dbSNP:779659334
2620 2620 a, g dbSNP:542187592
2642 2642 c, t dbSNP:560553573
2651 2651 a, c dbSNP:754643206
2689 2689 -, t dbSNP:765841457
2732 2732 a, t dbSNP:773962924
2746 2746 c, t dbSNP:528133324
2759 2759 a, g dbSNP:575941477
2768 2768 a, g dbSNP:780752078
2780 2780 a, g dbSNP:747961605
2791 2791 a, g dbSNP:546665672
2807 2807 c, t dbSNP:564877825
2817 2817 a, g dbSNP:761701572
2883 2883 c, t dbSNP:546078262
2884 2884 a, g dbSNP:2227426
2887 2887 a, t dbSNP:371670019
2936 2936 c, t dbSNP:191481721
2953 2953 a, c dbSNP:550829516
3032 3032 c, t dbSNP:2059502
3065 3065 c, t dbSNP:1044291
3122 3122 c, g dbSNP:549354357
3125 3125 a, g dbSNP:571588950
3132 3132 a, t dbSNP:2059503
3156 3156 c, g dbSNP:560650086
3186 3186 c, t dbSNP:765773409
3251 3251 c, t dbSNP:370220237
3253 3253 a, g dbSNP:760784701
3267 3267 g, t dbSNP:116381809
3271 3271 g, t dbSNP:143937445
3286 3286 c, t dbSNP:776780011
3302 3302 c, g dbSNP:762237743
3322 3322 a, t dbSNP:553406972
3336 3336 c, t dbSNP:754536691
3348 3348 a, c dbSNP:778641259
3365 3365 a, c dbSNP:3183998
3406 3406 c, t dbSNP:527823691
3407 3407 a, g dbSNP:765456260
3419 3419 a, c dbSNP:578060711
3445 3445 a, c dbSNP:538812066
3493 3493 c, t dbSNP:183622020
3503 3503 a, t dbSNP:145761175
3507 3507 c, t dbSNP:542921856
3517 3517 c, t dbSNP:561466155
3555 3555 a, g dbSNP:750815526
3587 3587 c, t dbSNP:763512099
3589 3589 c, t dbSNP:767029064
3595 3595 a, c dbSNP:751086921

Target ORF information:

RefSeq Version NM_005141
Organism Homo sapiens (human)
Definition Homo sapiens fibrinogen beta chain (FGB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005141

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAAAAGGA TGGTTTCTTG GAGCTTCCAC AAACTTAAAA CCATGAAACA TCTATTATTG 
CTACTATTGT GTGTTTTTCT AGTTAAGTCC CAAGGTGTCA ACGACAATGA GGAGGGTTTC
TTCAGTGCCC GTGGTCATCG ACCCCTTGAC AAGAAGAGAG AAGAGGCTCC CAGCCTGAGG
CCTGCCCCAC CGCCCATCAG TGGAGGTGGC TATCGGGCTC GTCCAGCCAA AGCAGCTGCC
ACTCAAAAGA AAGTAGAAAG AAAAGCCCCT GATGCTGGAG GCTGTCTTCA CGCTGACCCA
GACCTGGGGG TGTTGTGTCC TACAGGATGT CAGTTGCAAG AGGCTTTGCT ACAACAGGAA
AGGCCAATCA GAAATAGTGT TGATGAGTTA AATAACAATG TGGAAGCTGT TTCCCAGACC
TCCTCTTCTT CCTTTCAGTA CATGTATTTG CTGAAAGACC TGTGGCAAAA GAGGCAGAAG
CAAGTAAAAG ATAATGAAAA TGTAGTCAAT GAGTACTCCT CAGAACTGGA AAAGCACCAA
TTATATATAG ATGAGACTGT GAATAGCAAT ATCCCAACTA ACCTTCGTGT GCTTCGTTCA
ATCCTGGAAA ACCTGAGAAG CAAAATACAA AAGTTAGAAT CTGATGTCTC AGCTCAAATG
GAATATTGTC GCACCCCATG CACTGTCAGT TGCAATATTC CTGTGGTGTC TGGCAAAGAA
TGTGAGGAAA TTATCAGGAA AGGAGGTGAA ACATCTGAAA TGTATCTCAT TCAACCTGAC
AGTTCTGTCA AACCGTATAG AGTATACTGT GACATGAATA CAGAAAATGG AGGATGGACA
GTGATTCAGA ACCGTCAAGA CGGTAGTGTT GACTTTGGCA GGAAATGGGA TCCATATAAA
CAGGGATTTG GAAATGTTGC AACCAACACA GATGGGAAGA ATTACTGTGG CCTACCAGGT
GAATATTGGC TTGGAAATGA TAAAATTAGC CAGCTTACCA GGATGGGACC CACAGAACTT
TTGATAGAAA TGGAGGACTG GAAAGGAGAC AAAGTAAAGG CTCACTATGG AGGATTCACT
GTACAGAATG AAGCCAACAA ATACCAGATC TCAGTGAACA AATACAGAGG AACAGCCGGT
AATGCCCTCA TGGATGGAGC ATCTCAGCTG ATGGGAGAAA ACAGGACCAT GACCATTCAC
AACGGCATGT TCTTCAGCAC GTATGACAGA GACAATGACG GCTGGTTAAC ATCAGATCCC
AGAAAACAGT GTTCTAAAGA AGACGGTGGT GGATGGTGGT ATAATAGATG TCATGCAGCC
AATCCAAACG GCAGATACTA CTGGGGTGGA CAGTACACCT GGGACATGGC AAAGCATGGC
ACAGATGATG GTGTAGTATG GATGAATTGG AAGGGGTCAT GGTACTCAAT GAGGAAGATG
AGTATGAAGA TCAGGCCCTT CTTCCCACAG CAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28452
Accession Version NM_001184741.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibrinogen beta chain isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312972.1, BC036007.1 and AC107385.4. Summary: The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036007.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAAAAGGA TGGTTTCTTG GAGCTTCCAC AAACTTAAAA CCATGAAACA TCTATTATTG 
CTACTATTGT GTGTTTTTCT AGTTAAGTCC CAAGGTGTCA ACGACAATGA GGAGGGTTTC
TTCAGTGCCC GTGGTCATCG ACCCCTTGAC AAGAAGAGAG AAGAGGCTTT GCTACAACAG
GAAAGGCCAA TCAGAAATAG TGTTGATGAG TTAAATAACA ATGTGGAAGC TGTTTCCCAG
ACCTCCTCTT CTTCCTTTCA GTACATGTAT TTGCTGAAAG ACCTGTGGCA AAAGAGGCAG
AAGCAAGTAA AAGATAATGA AAATGTAGTC AATGAGTACT CCTCAGAACT GGAAAAGCAC
CAATTATATA TAGATGAGAC TGTGAATAGC AATATCCCAA CTAACCTTCG TGTGCTTCGT
TCAATCCTGG AAAACCTGAG AAGCAAAATA CAAAAGTTAG AATCTGATGT CTCAGCTCAA
ATGGAATATT GTCGCACCCC ATGCACTGTC AGTTGCAATA TTCCTGTGGT GTCTGGCAAA
GAATGTGAGG AAATTATCAG GAAAGGAGGT GAAACATCTG AAATGTATCT CATTCAACCT
GACAGTTCTG TCAAACCGTA TAGAGTATAC TGTGACATGA ATACAGAAAA TGGAGGATGG
ACAGTGATTC AGAACCGTCA AGACGGTAGT GTTGACTTTG GCAGGAAATG GGATCCATAT
AAACAGGGAT TTGGAAATGT TGCAACCAAC ACAGATGGGA AGAATTACTG TGGCCTACCA
GGTGAATATT GGCTTGGAAA TGATAAAATT AGCCAGCTTA CCAGGATGGG ACCCACAGAA
CTTTTGATAG AAATGGAGGA CTGGAAAGGA GACAAAGTAA AGGCTCACTA TGGAGGATTC
ACTGTACAGA ATGAAGCCAA CAAATACCAG ATCTCAGTGA ACAAATACAG AGGAACAGCC
GGTAATGCCC TCATGGATGG AGCATCTCAG CTGATGGGAG AAAACAGGAC CATGACCATT
CACAACGGCA TGTTCTTCAG CACGTATGAC AGAGACAATG ACGGCTGGTT AACATCAGAT
CCCAGAAAAC AGTGTTCTAA AGAAGACGGT GGTGGATGGT GGTATAATAG ATGTCATGCA
GCCAATCCAA ACGGCAGATA CTACTGGGGT GGACAGTACA CCTGGGACAT GGCAAAGCAT
GGCACAGATG ATGGTGTAGT ATGGATGAAT TGGAAGGGGT CATGGTACTC AATGAGGAAG
ATGAGTATGA AGATCAGGCC CTTCTTCCCA CAGCAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001171670.1
CDS40..1338
Misc Feature(1)31..33(+)
Misc Feature(2)208..567(+)
Misc Feature(3)574..1323(+)
Misc Feature(4)967..969(+)
Misc Feature(5)1093..1107(+)
Misc Feature(6)1132..1176(+)
Exon (1)1..153
Gene:FGB
Gene Synonym:
Exon (2)154..204
Gene:FGB
Gene Synonym:
Exon (3)205..352
Gene:FGB
Gene Synonym:
Exon (4)353..580
Gene:FGB
Gene Synonym:
Exon (5)581..694
Gene:FGB
Gene Synonym:
Exon (6)695..820
Gene:FGB
Gene Synonym:
Exon (7)821..1106
Gene:FGB
Gene Synonym:
Exon (8)1107..3451
Gene:FGB
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, g dbSNP:564463049
10 10 c, t dbSNP:768398827
12 12 c, g dbSNP:776370184
15 15 c, t dbSNP:761398967
25 25 c, g dbSNP:576348167
28 28 a, c dbSNP:375151084
31 31 a, g, t dbSNP:113961753
34 34 -, gt dbSNP:757727864
34 34 a, g dbSNP:36203394
39 39 c, t dbSNP:543846564
40 40 a, g dbSNP:751892977
43 43 a, g dbSNP:6053
49 49 a, t dbSNP:767891657
56 56 c, g dbSNP:752925190
70 70 a, g dbSNP:756304323
73 73 c, g dbSNP:777895153
75 75 a, t dbSNP:376146726
105 105 a, c dbSNP:758377428
118 118 c, t dbSNP:11099960
123 123 a, t dbSNP:375340782
125 125 a, g dbSNP:746963310
136 136 a, g dbSNP:562142566
141 141 c, t dbSNP:780849917
142 142 a, g dbSNP:747783086
143 143 a, g dbSNP:774271682
148 148 a, g dbSNP:769395119
162 162 c, t dbSNP:770388453
164 164 a, g dbSNP:773855571
169 169 c, t dbSNP:121909616
170 170 a, g dbSNP:746361316
172 172 g, t dbSNP:121909618
178 178 c, t dbSNP:121909625
179 179 a, g dbSNP:775891227
180 180 a, g dbSNP:6059
187 187 a, g dbSNP:760992086
194 194 a, g dbSNP:764538840
196 196 a, g dbSNP:776731258
201 201 a, g dbSNP:762126974
205 205 c, g dbSNP:751143215
213 213 a, g dbSNP:199768390
219 219 a, g dbSNP:755412339
225 225 a, g dbSNP:570183893
229 229 a, g dbSNP:148045912
232 232 a, g dbSNP:746024226
233 233 a, g dbSNP:748690444
244 244 a, g dbSNP:770246669
253 253 a, g dbSNP:142641543
259 259 a, g dbSNP:749412950
263 263 c, t dbSNP:771243649
264 264 c, g dbSNP:774263206
269 269 c, g dbSNP:759710385
302 302 a, c dbSNP:772160258
308 308 a, g dbSNP:776510812
317 317 a, g dbSNP:761740955
322 322 c, t dbSNP:764952511
338 338 a, t dbSNP:199596249
339 339 a, g dbSNP:762504717
341 341 a, g dbSNP:113610714
345 345 a, g dbSNP:766099099
346 346 g, t dbSNP:751016082
347 347 c, t dbSNP:754492004
363 363 c, t dbSNP:767079997
370 370 a, c dbSNP:2227409
372 372 a, t dbSNP:149963684
373 373 c, g dbSNP:764709626
375 375 a, g dbSNP:754323874
384 384 a, g dbSNP:757599938
387 387 a, c dbSNP:779081524
393 393 a, g dbSNP:201525782
396 396 c, g, t dbSNP:201909029
397 397 c, t dbSNP:543323660
402 402 a, c, g dbSNP:747127658
405 405 a, g dbSNP:777720766
409 409 a, g dbSNP:749217408
414 414 c, t dbSNP:770695888
421 421 a, g dbSNP:377741367
426 426 c, t dbSNP:759250713
429 429 c, t dbSNP:6056
436 436 c, g dbSNP:1043726
439 439 a, g dbSNP:775110705
441 441 g, t dbSNP:141978904
444 444 c, t dbSNP:780364193
448 448 c, t dbSNP:121909623
449 449 a, g dbSNP:530036524
455 455 c, t dbSNP:754302976
457 457 c, t dbSNP:747314723
458 458 a, g dbSNP:548206997
465 465 c, t dbSNP:150838423
467 467 a, t dbSNP:121909624
468 468 a, g dbSNP:1043727
486 486 a, c dbSNP:758583316
487 487 a, c dbSNP:139311055
488 488 c, t dbSNP:538708636
493 493 a, g dbSNP:374363120
496 496 c, t dbSNP:751805410
510 510 c, g dbSNP:755049679
512 512 c, t dbSNP:781138401
516 516 c, g, t dbSNP:749194615
518 518 a, g dbSNP:778766319
519 519 a, g dbSNP:745699048
532 532 c, t dbSNP:772014512
533 533 a, g dbSNP:775243595
544 544 a, g dbSNP:149599496
545 545 c, t dbSNP:564278470
550 550 a, c, t dbSNP:776059376
555 555 c, t dbSNP:776862282
560 560 g, t dbSNP:765701687
562 562 c, t dbSNP:750761651
568 568 g, t dbSNP:763257449
569 569 c, t dbSNP:144336893
608 608 g, t dbSNP:369272403
611 611 a, g dbSNP:759800033
613 613 a, g dbSNP:541789688
614 614 c, t dbSNP:776988939
619 619 c, g dbSNP:756100115
624 624 a, g dbSNP:201536638
626 626 a, g dbSNP:750327950
632 632 c, t dbSNP:758467916
642 642 c, g dbSNP:779988201
648 648 c, t dbSNP:746815835
654 654 a, g dbSNP:199550432
656 656 c, t dbSNP:6054
657 657 a, g dbSNP:144912407
659 659 a, g dbSNP:370703973
668 668 a, c dbSNP:772727358
669 669 c, t dbSNP:749785846
678 678 g, t dbSNP:147961877
690 690 c, t dbSNP:771496034
704 704 c, t dbSNP:771406104
705 705 g, t dbSNP:6058
706 706 a, t dbSNP:746420120
716 716 a, g dbSNP:748781687
717 717 c, t dbSNP:772265368
718 718 c, t dbSNP:775981072
723 723 c, t dbSNP:146831181
724 724 a, g dbSNP:769036186
729 729 c, t dbSNP:773739999
741 741 c, t dbSNP:761932805
751 751 a, g dbSNP:759172132
753 753 a, t dbSNP:199592944
754 754 c, t dbSNP:765435305
756 756 a, g dbSNP:200755352
761 761 a, g dbSNP:759534465
764 764 a, t dbSNP:767207616
768 768 a, t dbSNP:142064127
778 778 a, g dbSNP:572344189
782 782 c, t dbSNP:777451745
785 785 a, c dbSNP:753513033
787 787 a, t dbSNP:181245987
788 788 a, g dbSNP:778498115
792 792 a, g dbSNP:746345704
795 795 g, t dbSNP:151153332
797 797 a, g dbSNP:780249202
806 806 a, c dbSNP:564407258
813 813 c, t dbSNP:140191144
818 818 c, t dbSNP:374463429
825 825 a, g dbSNP:781702393
835 835 g, t dbSNP:748371656
837 837 a, t dbSNP:770123797
841 841 g, t dbSNP:368867072
859 859 a, g dbSNP:749434722
863 863 a, g dbSNP:770938745
887 887 c, t dbSNP:766420576
888 888 a, g, t dbSNP:751610009
889 889 a, g dbSNP:763862526
893 893 c, t dbSNP:150330219
897 897 a, g dbSNP:755184215
907 907 a, g dbSNP:761339656
908 908 c, g dbSNP:764989450
912 912 c, t dbSNP:547765642
913 913 -, aaagt dbSNP:761057382
920 920 a, g dbSNP:763287118
921 921 c, g dbSNP:765808222
927 927 -, caaaaa, caaaaataaaattt dbSNP:764656753
929 929 -, aaattt dbSNP:762214685
931 931 a, g dbSNP:752051727
934 934 a, g dbSNP:755602626
941 941 c, t dbSNP:781689806
943 943 g, t dbSNP:748566219
954 954 a, g dbSNP:753038061
955 955 a, g dbSNP:121909617
972 972 c, g, t dbSNP:756271964
975 975 a, g dbSNP:6057
979 979 a, t dbSNP:141025407
983 983 a, c dbSNP:774429340
987 987 c, t dbSNP:4681
990 990 a, g dbSNP:749738413
994 994 a, c dbSNP:776386995
999 999 c, t dbSNP:371254124
1000 1000 a, g dbSNP:764970021
1002 1002 c, t dbSNP:771357492
1005 1005 c, t dbSNP:778439014
1009 1009 c, g dbSNP:762523152
1010 1010 g, t dbSNP:121909621
1011 1011 c, t dbSNP:549444448
1014 1014 g, t dbSNP:765976401
1024 1024 a, t dbSNP:751184886
1034 1034 c, t dbSNP:138760475
1037 1037 c, g dbSNP:768192369
1041 1041 a, g dbSNP:753167823
1045 1045 a, g dbSNP:569380660
1047 1047 -, gaccat dbSNP:765705017
1051 1051 a, g dbSNP:200112613
1052 1052 c, t dbSNP:62637604
1053 1053 a, g dbSNP:140466984
1061 1061 a, c dbSNP:754035448
1065 1065 a, c, t dbSNP:771728339
1066 1066 a, g dbSNP:200792588
1083 1083 a, g dbSNP:143651398
1089 1089 c, t dbSNP:768641694
1096 1096 a, t dbSNP:776578431
1101 1101 c, t dbSNP:148950857
1102 1102 a, g dbSNP:141881199
1111 1111 a, g dbSNP:530461300
1114 1114 a, t dbSNP:749089748
1115 1115 c, t dbSNP:770876148
1119 1119 a, t dbSNP:774106857
1120 1120 c, g dbSNP:759268165
1145 1145 a, g dbSNP:553753567
1146 1146 c, t dbSNP:771651132
1147 1147 a, g dbSNP:199833798
1149 1149 c, t dbSNP:369472452
1151 1151 a, g dbSNP:121909622
1154 1154 g, t dbSNP:779328318
1167 1167 c, t dbSNP:745365985
1171 1171 c, t dbSNP:764555923
1173 1173 a, t dbSNP:758044306
1179 1179 a, t dbSNP:548663056
1187 1187 c, t dbSNP:762125715
1191 1191 c, t dbSNP:150650205
1200 1200 c, t dbSNP:528172101
1203 1203 c, t dbSNP:754698865
1205 1205 g, t dbSNP:199846554
1207 1207 c, g dbSNP:758674997
1216 1216 c, t dbSNP:766473963
1218 1218 c, t dbSNP:751634292
1228 1228 a, g dbSNP:756060132
1231 1231 a, g dbSNP:777526125
1238 1238 a, g dbSNP:749273737
1240 1240 a, g dbSNP:200163097
1250 1250 a, g dbSNP:112136436
1254 1254 a, t dbSNP:779851430
1268 1268 a, g dbSNP:202102031
1271 1271 a, g dbSNP:374178110
1272 1272 a, g dbSNP:113985604
1278 1278 g, t dbSNP:745503243
1281 1281 a, t dbSNP:571421762
1287 1287 c, t dbSNP:139212482
1292 1292 c, t dbSNP:558143752
1293 1293 a, g dbSNP:201307744
1295 1295 a, g dbSNP:4220
1302 1302 a, g dbSNP:762320286
1306 1306 a, g dbSNP:765571602
1309 1309 a, c dbSNP:773632388
1312 1312 a, c dbSNP:763133457
1320 1320 c, t dbSNP:766560796
1326 1326 c, t dbSNP:751830311
1348 1348 c, g, t dbSNP:376861907
1349 1349 a, g dbSNP:747422456
1353 1353 a, c, g dbSNP:375561591
1369 1369 a, g dbSNP:745703563
1374 1374 -, aca dbSNP:758707026
1374 1374 a, g dbSNP:758231685
1380 1380 a, t dbSNP:779529144
1386 1386 c, t dbSNP:746673921
1387 1387 a, g dbSNP:370076235
1571 1571 g, t dbSNP:539731011
1582 1582 g, t dbSNP:574115053
1601 1601 a, g dbSNP:141024945
1630 1630 g, t dbSNP:776523403
1669 1669 a, c dbSNP:559920720
1687 1687 a, g dbSNP:188621081
1700 1700 c, t dbSNP:747930205
1701 1701 a, g dbSNP:545671394
1708 1708 c, t dbSNP:150273093
1711 1711 a, g dbSNP:2227420
1747 1747 c, t dbSNP:192770089
1760 1760 a, c dbSNP:2227421
1767 1767 g, t dbSNP:759655332
1781 1781 a, g dbSNP:2227422
1785 1785 c, t dbSNP:2227423
1788 1788 a, t dbSNP:183181144
1796 1796 a, c dbSNP:543668072
1810 1810 a, g dbSNP:538497057
1826 1826 a, c dbSNP:374456153
1834 1834 a, g dbSNP:557290052
1846 1846 g, t dbSNP:139295173
1913 1913 c, t dbSNP:537427494
1929 1929 c, t dbSNP:760839598
1930 1930 a, c, g dbSNP:376470048
1940 1940 c, t dbSNP:555815994
1957 1957 c, t dbSNP:2227439
1962 1962 c, t dbSNP:754198978
1963 1963 a, g dbSNP:757615748
1991 1991 a, c dbSNP:372503293
2010 2010 a, g dbSNP:541521264
2024 2024 a, g dbSNP:375313300
2050 2050 a, g dbSNP:765764692
2156 2156 -, a dbSNP:368605323
2157 2157 -, a dbSNP:555952609
2172 2172 -, a dbSNP:57313183
2173 2173 -, g dbSNP:768727295
2184 2184 g, t dbSNP:541828079
2256 2256 c, g dbSNP:2227424
2286 2286 a, g dbSNP:372231882
2348 2348 c, t dbSNP:527937994
2365 2365 c, t dbSNP:563622201
2379 2379 a, t dbSNP:768949107
2408 2408 c, g dbSNP:2227425
2416 2416 c, t dbSNP:758981171
2417 2417 a, c dbSNP:187861993
2425 2425 c, t dbSNP:779659334
2443 2443 a, g dbSNP:542187592
2465 2465 c, t dbSNP:560553573
2474 2474 a, c dbSNP:754643206
2512 2512 -, t dbSNP:765841457
2555 2555 a, t dbSNP:773962924
2569 2569 c, t dbSNP:528133324
2582 2582 a, g dbSNP:575941477
2591 2591 a, g dbSNP:780752078
2603 2603 a, g dbSNP:747961605
2614 2614 a, g dbSNP:546665672
2630 2630 c, t dbSNP:564877825
2640 2640 a, g dbSNP:761701572
2706 2706 c, t dbSNP:546078262
2707 2707 a, g dbSNP:2227426
2710 2710 a, t dbSNP:371670019
2759 2759 c, t dbSNP:191481721
2776 2776 a, c dbSNP:550829516
2855 2855 c, t dbSNP:2059502
2888 2888 c, t dbSNP:1044291
2945 2945 c, g dbSNP:549354357
2948 2948 a, g dbSNP:571588950
2955 2955 a, t dbSNP:2059503
2979 2979 c, g dbSNP:560650086
3009 3009 c, t dbSNP:765773409
3074 3074 c, t dbSNP:370220237
3076 3076 a, g dbSNP:760784701
3090 3090 g, t dbSNP:116381809
3094 3094 g, t dbSNP:143937445
3109 3109 c, t dbSNP:776780011
3125 3125 c, g dbSNP:762237743
3145 3145 a, t dbSNP:553406972
3159 3159 c, t dbSNP:754536691
3171 3171 a, c dbSNP:778641259
3188 3188 a, c dbSNP:3183998
3229 3229 c, t dbSNP:527823691
3230 3230 a, g dbSNP:765456260
3242 3242 a, c dbSNP:578060711
3268 3268 a, c dbSNP:538812066
3316 3316 c, t dbSNP:183622020
3326 3326 a, t dbSNP:145761175
3330 3330 c, t dbSNP:542921856
3340 3340 c, t dbSNP:561466155
3378 3378 a, g dbSNP:750815526
3410 3410 c, t dbSNP:763512099
3412 3412 c, t dbSNP:767029064
3418 3418 a, c dbSNP:751086921

Target ORF information:

RefSeq Version NM_001184741
Organism Homo sapiens (human)
Definition Homo sapiens fibrinogen beta chain (FGB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001184741

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAAAAGGA TGGTTTCTTG GAGCTTCCAC AAACTTAAAA CCATGAAACA TCTATTATTG 
CTACTATTGT GTGTTTTTCT AGTTAAGTCC CAAGGTGTCA ACGACAATGA GGAGGGTTTC
TTCAGTGCCC GTGGTCATCG ACCCCTTGAC AAGAAGAGAG AAGAGGCTTT GCTACAACAG
GAAAGGCCAA TCAGAAATAG TGTTGATGAG TTAAATAACA ATGTGGAAGC TGTTTCCCAG
ACCTCCTCTT CTTCCTTTCA GTACATGTAT TTGCTGAAAG ACCTGTGGCA AAAGAGGCAG
AAGCAAGTAA AAGATAATGA AAATGTAGTC AATGAGTACT CCTCAGAACT GGAAAAGCAC
CAATTATATA TAGATGAGAC TGTGAATAGC AATATCCCAA CTAACCTTCG TGTGCTTCGT
TCAATCCTGG AAAACCTGAG AAGCAAAATA CAAAAGTTAG AATCTGATGT CTCAGCTCAA
ATGGAATATT GTCGCACCCC ATGCACTGTC AGTTGCAATA TTCCTGTGGT GTCTGGCAAA
GAATGTGAGG AAATTATCAG GAAAGGAGGT GAAACATCTG AAATGTATCT CATTCAACCT
GACAGTTCTG TCAAACCGTA TAGAGTATAC TGTGACATGA ATACAGAAAA TGGAGGATGG
ACAGTGATTC AGAACCGTCA AGACGGTAGT GTTGACTTTG GCAGGAAATG GGATCCATAT
AAACAGGGAT TTGGAAATGT TGCAACCAAC ACAGATGGGA AGAATTACTG TGGCCTACCA
GGTGAATATT GGCTTGGAAA TGATAAAATT AGCCAGCTTA CCAGGATGGG ACCCACAGAA
CTTTTGATAG AAATGGAGGA CTGGAAAGGA GACAAAGTAA AGGCTCACTA TGGAGGATTC
ACTGTACAGA ATGAAGCCAA CAAATACCAG ATCTCAGTGA ACAAATACAG AGGAACAGCC
GGTAATGCCC TCATGGATGG AGCATCTCAG CTGATGGGAG AAAACAGGAC CATGACCATT
CACAACGGCA TGTTCTTCAG CACGTATGAC AGAGACAATG ACGGCTGGTT AACATCAGAT
CCCAGAAAAC AGTGTTCTAA AGAAGACGGT GGTGGATGGT GGTATAATAG ATGTCATGCA
GCCAATCCAA ACGGCAGATA CTACTGGGGT GGACAGTACA CCTGGGACAT GGCAAAGCAT
GGCACAGATG ATGGTGTAGT ATGGATGAAT TGGAAGGGGT CATGGTACTC AATGAGGAAG
ATGAGTATGA AGATCAGGCC CTTCTTCCCA CAGCAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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