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Home » Species Summary » Homo sapiens » FGF3 cDNA ORF clone
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FGF3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol FGF3
Entrez Gene ID 2248
Full Name fibroblast growth factor 3
Synonyms HBGF-3, INT2
General protein information
Preferred Names
fibroblast growth factor 3
Names
fibroblast growth factor 3
FGF-3
oncogene INT2
proto-oncogene Int-2
INT-2 proto-oncogene protein
heparin-binding growth factor 3
murine mammary tumor virus integration site 2, mouse
V-INT2 murine mammary tumor virus integration site oncogene homolog
fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13

Summary The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

164950

Disorder Html: Deafness, congenital with inner ear agenesis, microtia, and

mRNA and Protein(s)

mRNA Protein Name
NM_005247 NP_005238 fibroblast growth factor 3 precursor

KEGG
hsa04010 MAPK signaling pathway
hsa04810 Regulation of actin cytoskeleton
hsa05218 Melanoma
hsa05200 Pathways in cancer
hsa04151 PI3K-Akt signaling pathway
hsa04014 Ras signaling pathway
hsa04015 Rap1 signaling pathway
Reactome
R-HSA-5663202 Diseases of signal transduction
R-HSA-1643685 Disease
R-HSA-1226099 Signaling by FGFR in disease
R-HSA-1839126 FGFR2 mutant receptor activation
R-HSA-2033519 Activated point mutants of FGFR2
R-HSA-5655253 Signaling by FGFR2 in disease
R-HSA-2219530 Constitutive Signaling by Aberrant PI3K in Cancer
R-HSA-2219528 PI3K/AKT Signaling in Cancer
R-HSA-168256 Immune System
R-HSA-1280218 Adaptive Immune System
R-HSA-1168372 Downstream signaling events of B Cell Receptor (BCR)
R-HSA-983705 Signaling by the B Cell Receptor (BCR)
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-168249 Innate Immune System
R-HSA-2172127 DAP12 interactions
R-HSA-2424491 DAP12 signaling
R-HSA-2454202 Fc epsilon receptor (FCERI) signaling
R-HSA-2871796 FCERI mediated MAPK activation
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-512988 Interleukin-3, 5 and GM-CSF signaling
R-HSA-449147 Signaling by Interleukins
R-HSA-2730905 Role of LAT2/NTAL/LAB on calcium mobilization
R-HSA-451927 Interleukin-2 signaling
R-HSA-912526 Interleukin receptor SHC signaling
R-HSA-162582 Signal Transduction
R-HSA-177929 Signaling by EGFR
R-HSA-179812 GRB2 events in EGFR signaling
R-HSA-180336 SHC1 events in EGFR signaling
R-HSA-180292 GAB1 signalosome
R-HSA-5654693 FRS-mediated FGFR1 signaling
R-HSA-5654688 SHC-mediated cascade:FGFR1
R-HSA-5654687 Downstream signaling of activated FGFR1
R-HSA-5654736 Signaling by FGFR1
R-HSA-5654726 Negative regulation of FGFR1 signaling
R-HSA-5654738 Signaling by FGFR2
R-HSA-190236 Signaling by FGFR
R-HSA-5654219 Phospholipase C-mediated cascade: FGFR1
R-HSA-5654741 Signaling by FGFR3
R-HSA-190242 FGFR1 ligand binding and activation
R-HSA-5654708 Downstream signaling of activated FGFR3
R-HSA-5654700 FRS-mediated FGFR2 signaling
R-HSA-190370 FGFR1b ligand binding and activation
R-HSA-5654689 PI-3K cascade:FGFR1
R-HSA-5654695 PI-3K cascade:FGFR2
R-HSA-190377 FGFR2b ligand binding and activation
R-HSA-5654743 Signaling by FGFR4
R-HSA-5654221 Phospholipase C-mediated cascade; FGFR2
R-HSA-5654699 SHC-mediated cascade:FGFR2
R-HSA-74751 Insulin receptor signalling cascade
R-HSA-112399 IRS-mediated signalling
R-HSA-190241 FGFR2 ligand binding and activation
R-HSA-5654712 FRS-mediated FGFR4 signaling
R-HSA-112412 SOS-mediated signalling
R-HSA-5654706 FRS-mediated FGFR3 signaling
R-HSA-167044 Signalling to RAS
R-HSA-5654720 PI-3K cascade:FGFR4
R-HSA-74752 Signaling by Insulin receptor
R-HSA-166520 Signalling by NGF
R-HSA-187037 NGF signalling via TRKA from the plasma membrane
R-HSA-109704 PI3K Cascade
R-HSA-187687 Signalling to ERKs
R-HSA-5654716 Downstream signaling of activated FGFR4
R-HSA-5654696 Downstream signaling of activated FGFR2
R-HSA-5654727 Negative regulation of FGFR2 signaling
R-HSA-186797 Signaling by PDGF
R-HSA-186763 Downstream signal transduction
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-198203 PI3K/AKT activation
R-HSA-194138 Signaling by VEGF
R-HSA-169893 Prolonged ERK activation events
R-HSA-170984 ARMS-mediated activation
R-HSA-170968 Frs2-mediated activation
R-HSA-5654710 PI-3K cascade:FGFR3
R-HSA-1250196 SHC1 events in ERBB2 signaling
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-1250347 SHC1 events in ERBB4 signaling
R-HSA-1963640 GRB2 events in ERBB2 signaling
R-HSA-1227986 Signaling by ERBB2
R-HSA-1963642 PI3K events in ERBB2 signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-187706 Signalling to p38 via RIT and RIN
R-HSA-1236394 Signaling by ERBB4
R-HSA-5218921 VEGFR2 mediated cell proliferation
R-HSA-1250342 PI3K events in ERBB4 signaling
R-HSA-372790 Signaling by GPCR
R-HSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK
R-HSA-2428928 IRS-related events triggered by IGF1R
R-HSA-2586552 Signaling by Leptin
R-HSA-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
R-HSA-2428924 IGF1R signaling cascade
R-HSA-1266738 Developmental Biology
R-HSA-375165 NCAM signaling for neurite out-growth
R-HSA-422475 Axon guidance
WikiPathways
WP51 Regulation of Actin Cytoskeleton


Homo sapiens (human) FGF3 NP_005238.1
Pan troglodytes (chimpanzee) FGF3 XP_522092.2
Macaca mulatta (Rhesus monkey) FGF3 XP_001100658.1
Bos taurus (cattle) FGF3 XP_002699485.1
Mus musculus (house mouse) Fgf3 NP_032033.2
Rattus norvegicus (Norway rat) Fgf3 NP_570830.1
Gallus gallus (chicken) FGF3 NP_990658.1
Danio rerio (zebrafish) fgf3 NP_571366.1
Xenopus (Silurana) tropicalis (western clawed frog) fgf3 NP_001008154.1

Component

ID Name Evidence
GO:0005576 extracellular region EXP
GO:0005576 extracellular region TAS
GO:0005737 cytoplasm IEA
GO:0005794 Golgi apparatus IEA

Function

ID Name Evidence
GO:0008083 growth factor activity IEA

Process

ID Name Evidence
GO:0001759 organ induction IEA
GO:0007165 signal transduction TAS
GO:0007267 cell-cell signaling TAS
GO:0008284 positive regulation of cell proliferation IGI
GO:0008286 insulin receptor signaling pathway TAS
GO:0008543 fibroblast growth factor receptor signaling pathway EXP
GO:0008543 fibroblast growth factor receptor signaling pathway IGI
GO:0008543 fibroblast growth factor receptor signaling pathway TAS
GO:0009653 anatomical structure morphogenesis TAS
GO:0030916 otic vesicle formation IEA
GO:0035121 tail morphogenesis IEA
GO:0048538 thymus development IEA
GO:0048752 semicircular canal morphogenesis IEA
GO:0051781 positive regulation of cell division IEA
GO:0055026 negative regulation of cardiac muscle tissue development IMP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FGF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18680 NM_005247 Homo sapiens fibroblast growth factor 3 (FGF3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu18680
Accession Version NM_005247.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-MAY-2014
Organism Homo sapiens (human)
Product fibroblast growth factor 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X14445.1. This sequence is a reference standard in the RefSeqGene project. On Sep 6, 2001 this sequence version replaced gi:4885232. Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC113739.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCCTAA TCTGGCTGCT ACTGCTCAGC CTGCTGGAGC CCGGCTGGCC CGCAGCGGGC 
CCTGGGGCGC GGTTGCGGCG CGATGCGGGC GGCCGTGGCG GCGTCTACGA GCACCTTGGC
GGGGCGCCCC GGCGCCGCAA GCTCTACTGC GCCACGAAGT ACCACCTCCA GCTGCACCCG
AGCGGCCGCG TCAACGGCAG CCTGGAGAAC AGCGCCTACA GTATTTTGGA GATAACGGCA
GTGGAGGTGG GCATTGTGGC CATCAGGGGT CTCTTCTCCG GGCGGTACCT GGCCATGAAC
AAGAGGGGAC GACTCTATGC TTCGGAGCAC TACAGCGCCG AGTGCGAGTT TGTGGAGCGG
ATCCACGAGC TGGGCTATAA TACGTATGCC TCCCGGCTGT ACCGGACGGT GTCTAGTACG
CCTGGGGCCC GCCGGCAGCC CAGCGCCGAG AGACTGTGGT ACGTGTCTGT GAACGGCAAG
GGCCGGCCCC GCAGGGGCTT CAAGACCCGC CGCACACAGA AGTCCTCCCT GTTCCTGCCC
CGCGTGCTGG ACCACAGGGA CCACGAGATG GTGCGGCAGC TACAGAGTGG GCTGCCCAGA
CCCCCTGGTA AGGGGGTCCA GCCCCGACGG CGGCGGCAGA AGCAGAGCCC GGATAACCTG
GAGCCCTCTC ACGTTCAGGC TTCGAGACTG GGCTCCCAGC TGGAGGCCAG TGCGCACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005238.1
CDS492..1211
Misc Feature(1)627..1034(+)
Misc Feature(2)627..1034(+)
Misc Feature(3)963..1013(+)
Exon (1)1..711
Gene:FGF3
Gene Synonym:
Exon (2)712..815
Gene:FGF3
Gene Synonym:
Exon (3)816..1548
Gene:FGF3
Gene Synonym:
Translation
Position Chain Variation Link
318 318 c, g dbSNP:12418223
508 508 c, t dbSNP:121917706
560 560 g, t dbSNP:41538178
637 637 a, g dbSNP:281860300
641 641 a, c dbSNP:281860301
687 687 g, t dbSNP:121917705
736 736 a, g dbSNP:146864055
742 742 -, g dbSNP:142716324
746 746 -, t dbSNP:281860302
774 774 c, t dbSNP:281860303
801 801 c, t dbSNP:121917704
808 808 a, g dbSNP:281860306
831 831 a, c, g dbSNP:147952743
836 836 c, g, t dbSNP:116162988
837 837 a, c, g dbSNP:79472069
885 885 -, c dbSNP:281860304
899 899 a, g dbSNP:35420992
948 948 -, tg dbSNP:281860307
957 957 c, t dbSNP:121917703
1009 1009 a, t dbSNP:7110221
1055 1055 a, c, t dbSNP:113473565
1076 1076 g, t dbSNP:115545058
1081 1081 a, g dbSNP:754817690
1107 1107 -, g dbSNP:281860305
1120 1120 a, g dbSNP:115452181
1162 1162 a, c dbSNP:751354293
1256 1256 a, g dbSNP:758169085

Target ORF information:

RefSeq Version NM_005247
Organism Homo sapiens (human)
Definition Homo sapiens fibroblast growth factor 3 (FGF3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005247

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGGCCTAA TCTGGCTGCT ACTGCTCAGC CTGCTGGAGC CCGGCTGGCC CGCAGCGGGC 
CCTGGGGCGC GGTTGCGGCG CGATGCGGGC GGCCGTGGCG GCGTCTACGA GCACCTTGGC
GGGGCGCCCC GGCGCCGCAA GCTCTACTGC GCCACGAAGT ACCACCTCCA GCTGCACCCG
AGCGGCCGCG TCAACGGCAG CCTGGAGAAC AGCGCCTACA GTATTTTGGA GATAACGGCA
GTGGAGGTGG GCATTGTGGC CATCAGGGGT CTCTTCTCCG GGCGGTACCT GGCCATGAAC
AAGAGGGGAC GACTCTATGC TTCGGAGCAC TACAGCGCCG AGTGCGAGTT TGTGGAGCGG
ATCCACGAGC TGGGCTATAA TACGTATGCC TCCCGGCTGT ACCGGACGGT GTCTAGTACG
CCTGGGGCCC GCCGGCAGCC CAGCGCCGAG AGACTGTGGT ACGTGTCTGT GAACGGCAAG
GGCCGGCCCC GCAGGGGCTT CAAGACCCGC CGCACACAGA AGTCCTCCCT GTTCCTGCCC
CGCGTGCTGG ACCACAGGGA CCACGAGATG GTGCGGCAGC TACAGAGTGG GCTGCCCAGA
CCCCCTGGTA AGGGGGTCCA GCCCCGACGG CGGCGGCAGA AGCAGAGCCC GGATAACCTG
GAGCCCTCTC ACGTTCAGGC TTCGAGACTG GGCTCCCAGC TGGAGGCCAG TGCGCACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Increased FGF3 and FGF4 gene dosage is a risk factor for craniosynostosis
Gene 534 (2), 435-439 (2014)
Grillo L, Greco D, Pettinato R, Avola E, Potenza N, Castiglia L, Spalletta A, Amata S, Di Benedetto D, Luciano D, Romano C and Fichera M.


book

Tooth agenesis association with self-reported family history of cancer
J. Dent. Res. 92 (2), 149-155 (2013)
Kuchler EC, Lips A, Tannure PN, Ho B, Costa MC, Granjeiro JM and Vieira AR.


book

Elevated FGF 23 and phosphorus are associated with coronary calcification in hemodialysis patients
Pediatr. Nephrol. 26 (6), 945-951 (2011)
Srivaths PR, Goldstein SL, Silverstein DM, Krishnamurthy R and Brewer ED.


book

LAMM syndrome with middle ear dysplasia associated with compound heterozygosity for FGF3 mutations
Am. J. Med. Genet. A 155A (5), 1096-1101 (2011)
Sensi A, Ceruti S, Trevisi P, Gualandi F, Busi M, Donati I, Neri M, Ferlini A and Martini A.


book

Variable expressivity of FGF3 mutations associated with deafness and LAMM syndrome
BMC Med. Genet. 12, 21 (2011)
Riazuddin S, Ahmed ZM, Hegde RS, Khan SN, Nasir I, Shaukat U, Riazuddin S, Butman JA, Griffith AJ, Friedman TB and Choi BY.


book

Mice homozygous for a targeted disruption of the proto-oncogene int-2 have developmental defects in the tail and inner ear
Development 117 (1), 13-28 (1993)
Mansour SL, Goddard JM and Capecchi MR.


book

Congenital Deafness with Labyrinthine Aplasia, Microtia, and Microdontia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Ordonez,J. and Tekin,M.


book

The int-2 proto-oncogene is responsible for induction of the inner ear
Nature 353 (6344), 561-563 (1991)
Represa J, Leon Y, Miner C and Giraldez F.


book

Sequence organization of the human int-2 gene and its expression in teratocarcinoma cells
Oncogene 4 (4), 429-436 (1989)
Brookes S, Smith R, Casey G, Dickson C and Peters G.


book

Characterization and chromosome assignment of the human homolog of int-2, a potential proto-oncogene
Mol. Cell. Biol. 6 (2), 502-510 (1986)
Casey,G., Smith,R., McGillivray,D., Peters,G. and Dickson,C.


 
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