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AAK1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol AAK1
Entrez Gene ID 22848
Full Name AP2 associated kinase 1
General protein information
Preferred Names
AP2-associated protein kinase 1
Names
AP2-associated protein kinase 1
adaptor-associated kinase 1
NP_055726.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p14

Summary Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

616405


mRNA and Protein(s)

mRNA Protein Name
NM_014911 NP_055726 AP2-associated protein kinase 1


Homo sapiens (human) AAK1 NP_055726.3
Pan troglodytes (chimpanzee) AAK1 XP_001138187.3
Canis lupus familiaris (dog) AAK1 XP_005626238.1
Mus musculus (house mouse) Aak1 NP_001035195.1
Rattus norvegicus (Norway rat) Aak1 NP_001166921.1
Gallus gallus (chicken) AAK1 XP_417663.4


The following AAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25045 NM_014911 Homo sapiens AP2 associated kinase 1 (AAK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25045
Clone ID Related Accession (Same CDS sequence) NM_014911
Accession Version NM_014911.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2886bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product AP2-associated protein kinase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW451954.1, DB285703.1, BC111965.1, DA121999.1, AW969393.1, AC136007.3, AC114772.5 and CA436375.1. On or before May 25, 2007 this sequence version replaced gi:113413001, gi:113413846, gi:29570779. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CTGGCCTGGG CTCCGGCTCC 
AGCGGAGGAG GGGGCAGCAC CTCGGGCCTG GGCAGTGGCT ACATCGGAAG AGTCTTCGGC
ATCGGGCGAC AGCAGGTCAC AGTGGACGAG GTGTTGGCGG AAGGTGGATT TGCTATTGTA
TTTCTGGTGA GGACAAGCAA TGGGATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGGGATCT TTCAGGGCAC
AAGAATATTG TGGGTTACAT TGATTCTAGT ATCAACAACG TGAGTAGCGG TGATGTATGG
GAAGTGCTCA TTCTGATGGA CTTTTGTAGA GGTGGCCAGG TGGTAAACCT GATGAACCAG
CGCCTGCAAA CAGGCTTTAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA TACCTGTGAA
GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGGGACCT GAAGGTTGAA
AACATCCTCT TGCATGACCG AGGCCACTAT GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
AAATTCCAGA ATCCACAAAC TGAGGGAGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
CCATTTGGGG AAAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAATTCT
CGATATTCTC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGACAAA
AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAGC TACTCAAGAA AGAGTGCCCA
ATTCCAAATG TACAGAACTC TCCCATTCCT GCAAAGCTTC CTGAACCAGT GAAAGCCAGT
GAGGCAGCTG CAAAAAAGAC CCAGCCAAAG GCCAGACTGA CAGATCCCAT TCCCACCACA
GAGACTTCAA TTGCACCCCG CCAGAGGCCT AAAGCTGGGC AGACTCAGCC GAACCCAGGA
ATCCTTCCCA TCCAGCCAGC GCTGACACCC CGGAAGAGGG CCACTGTTCA GCCCCCACCT
CAGGCTGCAG GATCCAGCAA TCAGCCTGGC CTTTTAGCCA GTGTTCCCCA ACCAAAACCC
CAAGCCCCAC CCAGCCAGCC TCTGCCGCAA ACTCAGGCCA AGCAGCCACA GGCTCCTCCC
ACTCCACAGC AGACGCCTTC TACTCAGGCC CAGGGTCTGC CCGCTCAGGC CCAGGCCACA
CCCCAGCACC AGCAGCAACT CTTCCTCAAG CAGCAACAGC AGCAGCAACA GCCACCGCCA
GCACAGCAGC AGCCGGCAGG CACGTTTTAC CAGCAGCAGC AGGCCCAGAC TCAGCAGTTT
CAGGCAGTAC ATCCAGCAAC CCAGCAACCA GCAATTGCTC AGTTCCCTGT GGTGTCCCAA
GGAGGCTCTC AACAGCAGCT AATGCAGAAT TTCTACCAGC AGCAGCAGCA GCAGCAACAA
CAACAGCAAC AGCAACAGCT GGCCACAGCC CTGCATCAAC AACAGCTGAT GACTCAGCAG
GCTGCCTTGC AGCAAAAGCC CACTATGGCA GCAGGACAGC AGCCCCAGCC ACAGCCAGCT
GCAGCCCCAC AGCCAGCCCC TGCCCAGGAG CCAGCGATTC AAGCCCCAGT AAGACAACAG
CCAAAGGTTC AGACAACCCC ACCTCCTGCC GTCCAGGGGC AGAAAGTTGG ATCTCTCACT
CCACCCTCAT CCCCCAAAAC CCAACGTGCT GGGCACAGGC GTATTCTCAG TGACGTAACC
CACAGTGCAG TCTTTGGGGT CCCTGCCAGC AAATCAACCC AGCTGCTCCA GGCAGCTGCA
GCTGAGGCCA GTCTCAATAA GTCCAAGTCT GCAACCACCA CTCCATCAGG CTCTCCTCGG
ACCTCTCAAC AAAACGTTTA TAATCCTTCA GAAGGGTCTA CGTGGAATCC CTTTGATGAC
GATAATTTCT CCAAACTCAC AGCTGAAGAA CTGCTAAACA AGGACTTTGC CAAGCTTGGG
GAAGGCAAAC ATCCCGAGAA GCTTGGAGGC TCAGCTGAGA GTTTGATCCC AGGCTTTCAA
TCAACCCAAG GTGATGCTTT TGCTACGACC TCATTTTCTG CTGGAACTGC TGAAAAAAGG
AAGGGTGGGC AGACTGTGGA CTCTGGCCTC CCGCTTCTAA GCGTGTCTGA TCCTTTCATT
CCTCTTCAAG TACCTGATGC ACCAGAAAAA CTAATTGAGG GACTCAAATC TCCTGACACT
TCTCTTCTGC TCCCTGACCT CTTGCCTATG ACAGATCCTT TTGGTAGCAC TTCTGATGCT
GTAATTGAAA AAGCTGATGT TGCTGTTGAG AGTCTCATAC CAGGACTGGA GCCCCCAGTT
CCCCAGCGCC TCCCATCTCA GACGGAATCT GTGACCTCGA ATCGCACAGA TTCTCTCACC
GGGGAAGATT CCCTGCTTGA TTGCTCTCTG CTCTCTAACC CTACTACTGA CCTTCTGGAA
GAGTTTGCCC CCACAGCAAT CTCTGCTCCA GTCCATAAAG CTGCAGAAGA TAGTAATCTC
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CTGAAGATGA GTTTGACCCT
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTGGGCACT CTAGAAACAG CAGTGGGAGC
TCTGAGTCCA GTCTTCCCAA CCTAGCCAGG TCTTTACTGC TGGTGGATCA GCTCATAGAC
CTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055726.3
CDS506..3391
Misc Feature(1)401..403(+)
Misc Feature(2)506..508(+)
Misc Feature(3)545..547(+)
Misc Feature(4)629..1453(+)
Misc Feature(5)644..1426(+)
Misc Feature(6)659..1177(+)
Misc Feature(7)659..1087(+)
Misc Feature(8)671..1177(+)
Misc Feature(9)1082..1177(+)
Misc Feature(10)1205..1207(+)
Misc Feature(11)1208..1210(+)
Misc Feature(12)1565..1567(+)
Misc Feature(13)1670..1672(+)
Misc Feature(14)1826..1828(+)
Misc Feature(15)2321..2323(+)
Misc Feature(16)2321..2323(+)
Misc Feature(17)2363..2365(+)
Misc Feature(18)2363..2365(+)
Misc Feature(19)2372..2374(+)
Misc Feature(20)2372..2374(+)
Misc Feature(21)2375..2377(+)
Misc Feature(22)2375..2377(+)
Misc Feature(23)2414..2416(+)
Misc Feature(24)2453..2455(+)
Misc Feature(25)2462..2464(+)
Misc Feature(26)2564..2566(+)
Misc Feature(27)2696..2698(+)
Exon (1)1..271
Gene:AAK1
Gene Synonym:
Exon (2)272..668
Gene:AAK1
Gene Synonym:
Exon (3)669..787
Gene:AAK1
Gene Synonym:
Exon (4)788..896
Gene:AAK1
Gene Synonym:
Exon (5)897..1039
Gene:AAK1
Gene Synonym:
Exon (6)1040..1161
Gene:AAK1
Gene Synonym:
Exon (7)1162..1243
Gene:AAK1
Gene Synonym:
Exon (8)1244..1376
Gene:AAK1
Gene Synonym:
Exon (9)1377..1480
Gene:AAK1
Gene Synonym:
Exon (10)1481..1560
Gene:AAK1
Gene Synonym:
Exon (11)1561..1715
Gene:AAK1
Gene Synonym:
Exon (12)1716..2002
Gene:AAK1
Gene Synonym:
Exon (13)2003..2281
Gene:AAK1
Gene Synonym:
Exon (14)2282..2511
Gene:AAK1
Gene Synonym:
Exon (15)2512..2669
Gene:AAK1
Gene Synonym:
Exon (16)2670..2774
Gene:AAK1
Gene Synonym:
Exon (17)2775..2870
Gene:AAK1
Gene Synonym:
Exon (18)2871..2972
Gene:AAK1
Gene Synonym:
Exon (19)2973..3074
Gene:AAK1
Gene Synonym:
Exon (20)3075..3185
Gene:AAK1
Gene Synonym:
Exon (21)3186..3296
Gene:AAK1
Gene Synonym:
Exon (22)3297..21265
Gene:AAK1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014911
Organism Homo sapiens (human)
Definition Homo sapiens AP2 associated kinase 1 (AAK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014911

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CTGGCCTGGG CTCCGGCTCC 
AGCGGAGGAG GGGGCAGCAC CTCGGGCCTG GGCAGTGGCT ACATCGGAAG AGTCTTCGGC
ATCGGGCGAC AGCAGGTCAC AGTGGACGAG GTGTTGGCGG AAGGTGGATT TGCTATTGTA
TTTCTGGTGA GGACAAGCAA TGGGATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGGGATCT TTCAGGGCAC
AAGAATATTG TGGGTTACAT TGATTCTAGT ATCAACAACG TGAGTAGCGG TGATGTATGG
GAAGTGCTCA TTCTGATGGA CTTTTGTAGA GGTGGCCAGG TGGTAAACCT GATGAACCAG
CGCCTGCAAA CAGGCTTTAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA TACCTGTGAA
GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGGGACCT GAAGGTTGAA
AACATCCTCT TGCATGACCG AGGCCACTAT GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
AAATTCCAGA ATCCACAAAC TGAGGGAGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
CCATTTGGGG AAAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAATTCT
CGATATTCTC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGACAAA
AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAGC TACTCAAGAA AGAGTGCCCA
ATTCCAAATG TACAGAACTC TCCCATTCCT GCAAAGCTTC CTGAACCAGT GAAAGCCAGT
GAGGCAGCTG CAAAAAAGAC CCAGCCAAAG GCCAGACTGA CAGATCCCAT TCCCACCACA
GAGACTTCAA TTGCACCCCG CCAGAGGCCT AAAGCTGGGC AGACTCAGCC GAACCCAGGA
ATCCTTCCCA TCCAGCCAGC GCTGACACCC CGGAAGAGGG CCACTGTTCA GCCCCCACCT
CAGGCTGCAG GATCCAGCAA TCAGCCTGGC CTTTTAGCCA GTGTTCCCCA ACCAAAACCC
CAAGCCCCAC CCAGCCAGCC TCTGCCGCAA ACTCAGGCCA AGCAGCCACA GGCTCCTCCC
ACTCCACAGC AGACGCCTTC TACTCAGGCC CAGGGTCTGC CCGCTCAGGC CCAGGCCACA
CCCCAGCACC AGCAGCAACT CTTCCTCAAG CAGCAACAGC AGCAGCAACA GCCACCGCCA
GCACAGCAGC AGCCGGCAGG CACGTTTTAC CAGCAGCAGC AGGCCCAGAC TCAGCAGTTT
CAGGCAGTAC ATCCAGCAAC CCAGCAACCA GCAATTGCTC AGTTCCCTGT GGTGTCCCAA
GGAGGCTCTC AACAGCAGCT AATGCAGAAT TTCTACCAGC AGCAGCAGCA GCAGCAACAA
CAACAGCAAC AGCAACAGCT GGCCACAGCC CTGCATCAAC AACAGCTGAT GACTCAGCAG
GCTGCCTTGC AGCAAAAGCC CACTATGGCA GCAGGACAGC AGCCCCAGCC ACAGCCAGCT
GCAGCCCCAC AGCCAGCCCC TGCCCAGGAG CCAGCGATTC AAGCCCCAGT AAGACAACAG
CCAAAGGTTC AGACAACCCC ACCTCCTGCC GTCCAGGGGC AGAAAGTTGG ATCTCTCACT
CCACCCTCAT CCCCCAAAAC CCAACGTGCT GGGCACAGGC GTATTCTCAG TGACGTAACC
CACAGTGCAG TCTTTGGGGT CCCTGCCAGC AAATCAACCC AGCTGCTCCA GGCAGCTGCA
GCTGAGGCCA GTCTCAATAA GTCCAAGTCT GCAACCACCA CTCCATCAGG CTCTCCTCGG
ACCTCTCAAC AAAACGTTTA TAATCCTTCA GAAGGGTCTA CGTGGAATCC CTTTGATGAC
GATAATTTCT CCAAACTCAC AGCTGAAGAA CTGCTAAACA AGGACTTTGC CAAGCTTGGG
GAAGGCAAAC ATCCCGAGAA GCTTGGAGGC TCAGCTGAGA GTTTGATCCC AGGCTTTCAA
TCAACCCAAG GTGATGCTTT TGCTACGACC TCATTTTCTG CTGGAACTGC TGAAAAAAGG
AAGGGTGGGC AGACTGTGGA CTCTGGCCTC CCGCTTCTAA GCGTGTCTGA TCCTTTCATT
CCTCTTCAAG TACCTGATGC ACCAGAAAAA CTAATTGAGG GACTCAAATC TCCTGACACT
TCTCTTCTGC TCCCTGACCT CTTGCCTATG ACAGATCCTT TTGGTAGCAC TTCTGATGCT
GTAATTGAAA AAGCTGATGT TGCTGTTGAG AGTCTCATAC CAGGACTGGA GCCCCCAGTT
CCCCAGCGCC TCCCATCTCA GACGGAATCT GTGACCTCGA ATCGCACAGA TTCTCTCACC
GGGGAAGATT CCCTGCTTGA TTGCTCTCTG CTCTCTAACC CTACTACTGA CCTTCTGGAA
GAGTTTGCCC CCACAGCAAT CTCTGCTCCA GTCCATAAAG CTGCAGAAGA TAGTAATCTC
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CTGAAGATGA GTTTGACCCT
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTGGGCACT CTAGAAACAG CAGTGGGAGC
TCTGAGTCCA GTCTTCCCAA CCTAGCCAGG TCTTTACTGC TGGTGGATCA GCTCATAGAC
CTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

AP-2-associated protein kinase 1 and cyclin G-associated kinase regulate hepatitis C virus entry and are potential drug targets
J. Virol. 89 (8), 4387-4404 (2015)
Neveu G, Ziv-Av A, Barouch-Bentov R, Berkerman E, Mulholland J and Einav S.


book

Genome-wide association study of a heart failure related metabolomic profile among African Americans in the Atherosclerosis Risk in Communities (ARIC) study
Genet. Epidemiol. 37 (8), 840-845 (2013)
Yu B, Zheng Y, Alexander D, Manolio TA, Alonso A, Nettleton JA and Boerwinkle E.


book

Identification of novel ATP13A2 interactors and their role in alpha-synuclein misfolding and toxicity
Hum. Mol. Genet. 21 (17), 3785-3794 (2012)
Usenovic M, Knight AL, Ray A, Wong V, Brown KR, Caldwell GA, Caldwell KA, Stagljar I and Krainc D.


book

The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway
J. Biol. Chem. 286 (21), 18720-18730 (2011)
Gupta-Rossi N, Ortica S, Meas-Yedid V, Heuss S, Moretti J, Olivo-Marin JC and Israel A.


book

Genomewide association study for onset age in Parkinson disease
BMC Med. Genet. 10, 98 (2009)
Latourelle JC, Pankratz N, Dumitriu A, Wilk JB, Goldwurm S, Pezzoli G, Mariani CB, DeStefano AL, Halter C, Gusella JF, Nichols WC, Myers RH and Foroud T.


book

AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrin
Traffic 4 (12), 885-890 (2003)
Conner SD, Schroter T and Schmid SL.


book

Differential requirements for AP-2 in clathrin-mediated endocytosis
J. Cell Biol. 162 (5), 773-779 (2003)
Conner SD and Schmid SL.


book

The protein kinase complement of the human genome
Science 298 (5600), 1912-1934 (2002)
Manning G, Whyte DB, Martinez R, Hunter T and Sudarsanam S.


book

Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis
J. Cell Biol. 156 (5), 921-929 (2002)
Conner SD and Schmid SL.


book

Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals
J. Cell Biol. 156 (5), 791-795 (2002)
Ricotta D, Conner SD, Schmid SL, von Figura K and Honing S.


 
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