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ALDOB cDNA ORF clone, Homo sapiens (human)

Gene Symbol ALDOB
Entrez Gene ID 229
Full Name aldolase B, fructose-bisphosphate
Synonyms ALDB, ALDO2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9q21.3-q22.2

Summary Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]. lac of sum
Disorder MIM:

612724

Disorder Html: Fructose intolerance, 229600 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000035 NP_000026 fructose-bisphosphate aldolase B



Homo sapiens (human) ALDOB NP_000026.2
Pan troglodytes (chimpanzee) ALDOB XP_001136159.1
Macaca mulatta (Rhesus monkey) ALDOB XP_001111095.1
Canis lupus familiaris (dog) ALDOB XP_867333.3
Bos taurus (cattle) ALDOB NP_001029657.1
Mus musculus (house mouse) Aldob NP_659152.1
Rattus norvegicus (Norway rat) Aldob NP_036628.2
Gallus gallus (chicken) ALDOB NP_001007978.1
Danio rerio (zebrafish) aldob NP_919348.3
Xenopus (Silurana) tropicalis (western clawed frog) aldob NP_989131.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALDOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALDOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18947 NM_000035 Homo sapiens aldolase B, fructose-bisphosphate (ALDOB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18947
Clone ID Related Accession (Same CDS sequence) NM_000035
Accession Version NM_000035.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1095bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fructose-bisphosphate aldolase B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02747.1, AL353621.19 and AW242415.1. This sequence is a reference standard in the RefSeqGene project. On Dec 19, 2008 this sequence version replaced gi:40354204. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02747.1, X01098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000026.2
CDS83..1177
Misc Feature(1)1..1(+)
Misc Feature(2)41..43(+)
Misc Feature(3)119..1111(+)
Misc Feature(4)182..994(+)
Misc Feature(5)413..865(+)
Misc Feature(6)770..772(+)
Misc Feature(7)1172..1174(+)
Exon (1)1..72
Gene:ALDOB
Gene Synonym:
Exon (2)73..194
Gene:ALDOB
Gene Synonym:
Exon (3)195..406
Gene:ALDOB
Gene Synonym:
Exon (4)407..461
Gene:ALDOB
Gene Synonym:
Exon (5)462..622
Gene:ALDOB
Gene Synonym:
Exon (6)623..706
Gene:ALDOB
Gene Synonym:
Exon (7)707..881
Gene:ALDOB
Gene Synonym:
Exon (8)882..1081
Gene:ALDOB
Gene Synonym:
Exon (9)1082..2426
Gene:ALDOB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000035
Organism Homo sapiens (human)
Definition Homo sapiens aldolase B, fructose-bisphosphate (ALDOB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000035

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Aldolase B knockdown prevents high glucose-induced methylglyoxal overproduction and cellular dysfunction in endothelial cells
PLoS ONE 7 (7), E41495 (2012)
Liu J, Mak TC, Banigesh A, Desai K, Wang R and Wu L.


book

Genome-wide association study identifies loci influencing concentrations of liver enzymes in plasma
Nat. Genet. 43 (11), 1131-1138 (2011)
Chambers JC, Zhang W, Sehmi J, Li X, Wass MN, Van der Harst P, Holm H, Sanna S, Kavousi M, Baumeister SE, Coin LJ, Deng G, Gieger C, Heard-Costa NL, Hottenga JJ, Kuhnel B, Kumar V, Lagou V, Liang L, Luan J, Vidal PM, Mateo Leach I, O'Reilly PF, Peden JF, Rahmioglu N, Soininen P, Speliotes EK, Yuan X, Thorleifsson G, Alizadeh BZ, Atwood LD, Borecki IB, Brown MJ, Charoen P, Cucca F, Das D, de Geus EJ, Dixon AL, Doring A, Ehret G, Eyjolfsson GI, Farrall M, Forouhi NG, Friedrich N, Goessling W, Gudbjartsson DF, Harris TB, Hartikainen AL, Heath S, Hirschfield GM, Hofman A, Homuth G, Hypponen E, Janssen HL, Johnson T, Kangas AJ, Kema IP, Kuhn JP, Lai S, Lathrop M, Lerch MM, Li Y, Liang TJ, Lin JP, Loos RJ, Martin NG, Moffatt MF, Montgomery GW, Munroe PB, Musunuru K, Nakamura Y, O'Donnell CJ, Olafsson I, Penninx BW, Pouta A, Prins BP, Prokopenko I, Puls R, Ruokonen A, Savolainen MJ, Schlessinger D, Schouten JN, Seedorf U, Sen-Chowdhry S, Siminovitch KA, Smit JH, Spector TD, Tan W, Teslovich TM, Tukiainen T, Uitterlinden AG, Van der Klauw MM, Vasan RS, Wallace C, Wallaschofski H, Wichmann HE, Willemsen G, Wurtz P, Xu C, Yerges-Armstrong LM, Abecasis GR, Ahmadi KR, Boomsma DI, Caulfield M, Cookson WO, van Duijn CM, Froguel P, Matsuda K, McCarthy MI, Meisinger C, Mooser V, Pietilainen KH, Schumann G, Snieder H, Sternberg MJ, Stolk RP, Thomas HC, Thorsteinsdottir U, Uda M, Waeber G, Wareham NJ, Waterworth DM, Watkins H, Whitfield JB, Witteman JC, Wolffenbuttel BH, Fox CS, Ala-Korpela M, Stefansson K, Vollenweider P, Volzke H, Schadt EE, Scott J, Jarvelin MR, Elliott P and Kooner JS.


book

Stabilization of the predominant disease-causing aldolase variant (A149P) with zwitterionic osmolytes
Biochemistry 50 (5), 663-671 (2011)
Stopa JD, Chandani S and Tolan DR.


book

Mutations in the promoter region of the aldolase B gene that cause hereditary fructose intolerance
J. Inherit. Metab. Dis. 33 (6), 715-725 (2010)
Coffee EM and Tolan DR.


book

Hereditary fructose intolerance: functional study of two novel ALDOB natural variants and characterization of a partial gene deletion
Hum. Mutat. 31 (12), 1294-1303 (2010)
Esposito G, Imperato MR, Ieno L, Sorvillo R, Benigno V, Parenti G, Parini R, Vitagliano L, Zagari A and Salvatore F.


book

A new aldolase B variant, N334K, is a common cause of hereditary fructose intolerance in Yugoslavia
Nucleic Acids Res. 18 (7), 1925 (1990)
Cross NC, Stojanov LM and Cox TM.


book

Molecular analysis of aldolase B genes in hereditary fructose intolerance
Lancet 335 (8685), 306-309 (1990)
Cross NC, de Franchis R, Sebastio G, Dazzo C, Tolan DR, Gregori C, Odievre M, Vidailhet M, Romano V and Mascali G.


book

Construction and expression of human aldolase A and B expression plasmids in Escherichia coli host
Biochim. Biophys. Acta 1007 (3), 334-342 (1989)
Sakakibara M, Takahashi I, Takasaki Y, Mukai T and Hori K.


book

Human aldolase B gene: characterization of the genomic aldolase B gene and analysis of sequences required for multiple polyadenylations
J. Biochem. 102 (5), 1043-1051 (1987)
Mukai T, Yatsuki H, Arai Y, Joh K, Matsuhashi S and Hori K.


book

Human aldolase isozyme gene: the structure of multispecies aldolase B mRNAs
Nucleic Acids Res. 13 (14), 5055-5069 (1985)
Sakakibara,M., Mukai,T., Yatsuki,H. and Hori,K.


 
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