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TARDBP cDNA ORF clone, Homo sapiens (human)

Gene Symbol TARDBP
Entrez Gene ID 23435
Full Name TAR DNA binding protein
Synonyms ALS10, TDP-43
General protein information
Preferred Names
TAR DNA-binding protein 43
Names
TAR DNA-binding protein 43
TAR DNA-binding protein-43
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.22

Summary HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

605078

Disorder Html: Amyotrophic lateral sclerosis 10, with or without FTD, 612069 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_007375 NP_031401 TAR DNA-binding protein 43


Homo sapiens (human) TARDBP NP_031401.1
Pan troglodytes (chimpanzee) TARDBP XP_514380.2
Macaca mulatta (Rhesus monkey) TARDBP XP_001102660.1
Canis lupus familiaris (dog) TARDBP XP_005618063.1
Bos taurus (cattle) TARDBP NP_001039950.2
Mus musculus (house mouse) Tardbp NP_663531.1
Gallus gallus (chicken) TARDBP NP_001026049.1
Danio rerio (zebrafish) tardbp NP_958884.1
Xenopus (Silurana) tropicalis (western clawed frog) tardbp NP_989054.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TARDBP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TARDBP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19093
NM_007375 Homo sapiens TAR DNA binding protein (TARDBP), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19093
Clone ID Related Accession (Same CDS sequence) NM_007375
Accession Version NM_007375.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product TAR DNA-binding protein 43
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI464234.1, CD703666.1, BC001487.2, AL050265.1, AL045296.1, BC059955.1, AF311304.1 and BM716882.1. This sequence is a reference standard in the RefSeqGene project. On or before Feb 23, 2004 this sequence version replaced gi:13654285, gi:19743843. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001487.2, AL050265.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCTGAAT ATATTCGGGT AACCGAAGAT GAGAACGATG AGCCCATTGA AATACCATCG 
GAAGACGATG GGACGGTGCT GCTCTCCACG GTTACAGCCC AGTTTCCAGG GGCGTGTGGG
CTTCGCTACA GGAATCCAGT GTCTCAGTGT ATGAGAGGTG TCCGGCTGGT AGAAGGAATT
CTGCATGCCC CAGATGCTGG CTGGGGAAAT CTGGTGTATG TTGTCAACTA TCCAAAAGAT
AACAAAAGAA AAATGGATGA GACAGATGCT TCATCAGCAG TGAAAGTGAA AAGAGCAGTC
CAGAAAACAT CCGATTTAAT AGTGTTGGGT CTCCCATGGA AAACAACCGA ACAGGACCTG
AAAGAGTATT TTAGTACCTT TGGAGAAGTT CTTATGGTGC AGGTCAAGAA AGATCTTAAG
ACTGGTCATT CAAAGGGGTT TGGCTTTGTT CGTTTTACGG AATATGAAAC ACAAGTGAAA
GTAATGTCAC AGCGACATAT GATAGATGGA CGATGGTGTG ACTGCAAACT TCCTAATTCT
AAGCAAAGCC AAGATGAGCC TTTGAGAAGC AGAAAAGTGT TTGTGGGGCG CTGTACAGAG
GACATGACTG AGGATGAGCT GCGGGAGTTC TTCTCTCAGT ACGGGGATGT GATGGATGTC
TTCATCCCCA AGCCATTCAG GGCCTTTGCC TTTGTTACAT TTGCAGATGA TCAGATTGCG
CAGTCTCTTT GTGGAGAGGA CTTGATCATT AAAGGAATCA GCGTTCATAT ATCCAATGCC
GAACCTAAGC ACAATAGCAA TAGACAGTTA GAAAGAAGTG GAAGATTTGG TGGTAATCCA
GGTGGCTTTG GGAATCAGGG TGGATTTGGT AATAGCAGAG GGGGTGGAGC TGGTTTGGGA
AACAATCAAG GTAGTAATAT GGGTGGTGGG ATGAACTTTG GTGCGTTCAG CATTAATCCA
GCCATGATGG CTGCCGCCCA GGCAGCACTA CAGAGCAGTT GGGGTATGAT GGGCATGTTA
GCCAGCCAGC AGAACCAGTC AGGCCCATCG GGTAATAACC AAAACCAAGG CAACATGCAG
AGGGAGCCAA ACCAGGCCTT CGGTTCTGGA AATAACTCTT ATAGTGGCTC TAATTCTGGT
GCAGCAATTG GTTGGGGATC AGCATCCAAT GCAGGGTCGG GCAGTGGTTT TAATGGAGGC
TTTGGCTCAA GCATGGATTC TAAGTCTTCT GGCTGGGGAA TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_031401.1
CDS135..1379
Misc Feature(1)129..131(+)
Misc Feature(2)405..407(+)
Misc Feature(3)408..410(+)
Misc Feature(4)447..677(+)
Misc Feature(5)450..659(+)
Misc Feature(6)603..605(+)
Misc Feature(7)705..917(+)
Misc Feature(8)708..917(+)
Misc Feature(9)711..890(+)
Misc Feature(10)1008..1010(+)
Exon (1)1..122
Gene:TARDBP
Gene Synonym:
Exon (2)123..372
Gene:TARDBP
Gene Synonym:
Exon (3)373..536
Gene:TARDBP
Gene Synonym:
Exon (4)537..677
Gene:TARDBP
Gene Synonym:
Exon (5)678..848
Gene:TARDBP
Gene Synonym:
Exon (6)849..4217
Gene:TARDBP
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007375
Organism Homo sapiens (human)
Definition Homo sapiens TAR DNA binding protein (TARDBP), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007375

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCTGAAT ATATTCGGGT AACCGAAGAT GAGAACGATG AGCCCATTGA AATACCATCG 
GAAGACGATG GGACGGTGCT GCTCTCCACG GTTACAGCCC AGTTTCCAGG GGCGTGTGGG
CTTCGCTACA GGAATCCAGT GTCTCAGTGT ATGAGAGGTG TCCGGCTGGT AGAAGGAATT
CTGCATGCCC CAGATGCTGG CTGGGGAAAT CTGGTGTATG TTGTCAACTA TCCAAAAGAT
AACAAAAGAA AAATGGATGA GACAGATGCT TCATCAGCAG TGAAAGTGAA AAGAGCAGTC
CAGAAAACAT CCGATTTAAT AGTGTTGGGT CTCCCATGGA AAACAACCGA ACAGGACCTG
AAAGAGTATT TTAGTACCTT TGGAGAAGTT CTTATGGTGC AGGTCAAGAA AGATCTTAAG
ACTGGTCATT CAAAGGGGTT TGGCTTTGTT CGTTTTACGG AATATGAAAC ACAAGTGAAA
GTAATGTCAC AGCGACATAT GATAGATGGA CGATGGTGTG ACTGCAAACT TCCTAATTCT
AAGCAAAGCC AAGATGAGCC TTTGAGAAGC AGAAAAGTGT TTGTGGGGCG CTGTACAGAG
GACATGACTG AGGATGAGCT GCGGGAGTTC TTCTCTCAGT ACGGGGATGT GATGGATGTC
TTCATCCCCA AGCCATTCAG GGCCTTTGCC TTTGTTACAT TTGCAGATGA TCAGATTGCG
CAGTCTCTTT GTGGAGAGGA CTTGATCATT AAAGGAATCA GCGTTCATAT ATCCAATGCC
GAACCTAAGC ACAATAGCAA TAGACAGTTA GAAAGAAGTG GAAGATTTGG TGGTAATCCA
GGTGGCTTTG GGAATCAGGG TGGATTTGGT AATAGCAGAG GGGGTGGAGC TGGTTTGGGA
AACAATCAAG GTAGTAATAT GGGTGGTGGG ATGAACTTTG GTGCGTTCAG CATTAATCCA
GCCATGATGG CTGCCGCCCA GGCAGCACTA CAGAGCAGTT GGGGTATGAT GGGCATGTTA
GCCAGCCAGC AGAACCAGTC AGGCCCATCG GGTAATAACC AAAACCAAGG CAACATGCAG
AGGGAGCCAA ACCAGGCCTT CGGTTCTGGA AATAACTCTT ATAGTGGCTC TAATTCTGGT
GCAGCAATTG GTTGGGGATC AGCATCCAAT GCAGGGTCGG GCAGTGGTTT TAATGGAGGC
TTTGGCTCAA GCATGGATTC TAAGTCTTCT GGCTGGGGAA TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Reduced cellular Ca(2+) availability enhances TDP-43 cleavage by apoptotic caspases
Biochim. Biophys. Acta 1843 (4), 725-734 (2014)
De Marco G, Lomartire A, Mandili G, Lupino E, Buccinna B, Ramondetti C, Moglia C, Novelli F, Piccinini M, Mostert M, Rinaudo MT, Chio A and Calvo A.


book

'Structural characterization of the minimal segment of TDP-43 competent for aggregation'
Arch. Biochem. Biophys. 545, 53-62 (2014)
Mompean M, Buratti E, Guarnaccia C, Brito RM, Chakrabartty A, Baralle FE and Laurents DV.


book

Axonal transport of TDP-43 mRNA granules is impaired by ALS-causing mutations
Neuron 81 (3), 536-543 (2014)
Alami NH, Smith RB, Carrasco MA, Williams LA, Winborn CS, Han SS, Kiskinis E, Winborn B, Freibaum BD, Kanagaraj A, Clare AJ, Badders NM, Bilican B, Chaum E, Chandran S, Shaw CE, Eggan KC, Maniatis T and Taylor JP.


book

TDP-43 in ALS: stay on target...almost there
Neuron 81 (3), 463-465 (2014)
Jovicic A and Gitler AD.


book

Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping
EMBO J. 20 (7), 1774-1784 (2001)
Buratti E, Dork T, Zuccato E, Pagani F, Romano M and Baralle FE.


book

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing
EMBO Rep. 1 (3), 287-292 (2000)
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R and Wiemann S.


book

Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle
EMBO J. 15 (3), 457-467 (1996)
Tokai N, Fujimoto-Nishiyama A, Toyoshima Y, Yonemura S, Tsukita S, Inoue J and Yamamota T.


book

Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs
J. Virol. 69 (6), 3584-3596 (1995)
Ou SH, Wu F, Harrich D, Garcia-Martinez LF and Gaynor RB.


book

TARDBP-Related Amyotrophic Lateral Sclerosis
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Harms,M.M., Miller,T.M. and Baloh,R.H.


book

Amyotrophic Lateral Sclerosis Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kinsley,L. and Siddique,T.


 
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