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CLCF1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CLCF1
Entrez Gene ID 23529
Full Name cardiotrophin-like cytokine factor 1
Synonyms BSF-3, BSF3, CISS2, CLC, NNT-1, NNT1, NR6
General protein information
Preferred Names
cardiotrophin-like cytokine factor 1
Names
cardiotrophin-like cytokine factor 1
novel neurotrophin-1
B-cell stimulating factor 3
B-cell stimulatory factor 3
B-cell-stimulating factor 3
CRLF1 associated cytokine-like factor 1
neurotrophin-1/B-cell stimulating factor-3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13.3

Summary This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

607672

Disorder Html: Cold-induced sweating syndrome 1, 610313 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_013246 NP_037378 cardiotrophin-like cytokine factor 1 isoform 1 precursor
NM_001166212 NP_001159684 cardiotrophin-like cytokine factor 1 isoform 2 precursor



Homo sapiens (human) CLCF1 NP_037378.1
Macaca mulatta (Rhesus monkey) CLCF1 XP_001106809.1
Canis lupus familiaris (dog) CLCF1 XP_540818.2
Bos taurus (cattle) CLCF1 XP_002699410.1
Mus musculus (house mouse) Clcf1 NP_064336.1
Rattus norvegicus (Norway rat) Clcf1 NP_997498.2
Gallus gallus (chicken) CLCF1 XP_003643835.2
Danio rerio (zebrafish) LOC562247 XP_690733.5
Xenopus (Silurana) tropicalis (western clawed frog) clcf1 NP_001016067.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CLCF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16287
NM_013246 Homo sapiens cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu19127
NM_001166212 Homo sapiens cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16287
Clone ID Related Accession (Same CDS sequence) NM_013246
Accession Version NM_013246.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cardiotrophin-like cytokine factor 1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM846622.1 and BC012939.1. On Jul 28, 2004 this sequence version replaced gi:7019350. Transcript Variant: This variant (1) encodes the longer isoform (1). Though this isoform has a predicted signal peptide at amino acids 1-27, experiments show it is retained within the cell. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012939.1, AF172854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG 
CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC
TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG
GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA
CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC
AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC
CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG
CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG
AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG
GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG
GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_037378.1
CDS197..874
Misc Feature(1)110..112(+)
Misc Feature(2)362..832(+)
Exon (1)1..212
Gene:CLCF1
Gene Synonym:
Exon (2)213..379
Gene:CLCF1
Gene Synonym:
Exon (3)380..1842
Gene:CLCF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013246
Organism Homo sapiens (human)
Definition Homo sapiens cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013246

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACCTCC GAGCAGGGGA CTCGTGGGGG ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG 
CACCTCCCTG CAGTGCCAGC TCTCAATCGC ACAGGGGACC CAGGGCCTGG CCCCTCCATC
CAGAAAACCT ATGACCTCAC CCGCTACCTG GAGCACCAAC TCCGCAGCTT GGCTGGGACC
TATCTGAACT ACCTGGGCCC CCCTTTCAAC GAGCCAGACT TCAACCCTCC CCGCCTGGGG
GCAGAGACTC TGCCCAGGGC CACTGTTGAC TTGGAGGTGT GGCGAAGCCT CAATGACAAA
CTGCGGCTGA CCCAGAACTA CGAGGCCTAC AGCCACCTTC TGTGTTACTT GCGTGGCCTC
AACCGTCAGG CTGCCACTGC TGAGCTGCGC CGCAGCCTGG CCCACTTCTG CACCAGCCTC
CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC ATGGCAGCTC TGGGCTACCC ACTGCCCCAG
CCGCTGCCTG GGACTGAACC CACTTGGACT CCTGGCCCTG CCCACAGTGA CTTCCTCCAG
AAGATGGACG ACTTCTGGCT GCTGAAGGAG CTGCAGACCT GGCTGTGGCG CTCGGCCAAG
GACTTCAACC GGCTCAAGAA GAAGATGCAG CCTCCAGCAG CTGCAGTCAC CCTGCACCTG
GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19127
Clone ID Related Accession (Same CDS sequence) NM_001166212
Accession Version NM_001166212.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 648bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cardiotrophin-like cytokine factor 1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC393345.1, AK298052.1 and BC012939.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298052.1, BX497225.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA2145240 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG CACCTCCCTG CAGTGCCAGC TCTCAATCGC 
ACAGGGGACC CAGGGCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG
GAGCACCAAC TCCGCAGCTT GGCTGGGACC TATCTGAACT ACCTGGGCCC CCCTTTCAAC
GAGCCAGACT TCAACCCTCC CCGCCTGGGG GCAGAGACTC TGCCCAGGGC CACTGTTGAC
TTGGAGGTGT GGCGAAGCCT CAATGACAAA CTGCGGCTGA CCCAGAACTA CGAGGCCTAC
AGCCACCTTC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACTGC TGAGCTGCGC
CGCAGCCTGG CCCACTTCTG CACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC
ATGGCAGCTC TGGGCTACCC ACTGCCCCAG CCGCTGCCTG GGACTGAACC CACTTGGACT
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGACG ACTTCTGGCT GCTGAAGGAG
CTGCAGACCT GGCTGTGGCG CTCGGCCAAG GACTTCAACC GGCTCAAGAA GAAGATGCAG
CCTCCAGCAG CTGCAGTCAC CCTGCACCTG GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159684.1
CDS146..793
Misc Feature(1)281..751(+)
Exon (1)1..131
Gene:CLCF1
Gene Synonym:
Exon (2)132..298
Gene:CLCF1
Gene Synonym:
Exon (3)299..1761
Gene:CLCF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166212
Organism Homo sapiens (human)
Definition Homo sapiens cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166212

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTTAGCGT GCCTGTGCAC GGTGCTCTGG CACCTCCCTG CAGTGCCAGC TCTCAATCGC 
ACAGGGGACC CAGGGCCTGG CCCCTCCATC CAGAAAACCT ATGACCTCAC CCGCTACCTG
GAGCACCAAC TCCGCAGCTT GGCTGGGACC TATCTGAACT ACCTGGGCCC CCCTTTCAAC
GAGCCAGACT TCAACCCTCC CCGCCTGGGG GCAGAGACTC TGCCCAGGGC CACTGTTGAC
TTGGAGGTGT GGCGAAGCCT CAATGACAAA CTGCGGCTGA CCCAGAACTA CGAGGCCTAC
AGCCACCTTC TGTGTTACTT GCGTGGCCTC AACCGTCAGG CTGCCACTGC TGAGCTGCGC
CGCAGCCTGG CCCACTTCTG CACCAGCCTC CAGGGCCTGC TGGGCAGCAT TGCGGGCGTC
ATGGCAGCTC TGGGCTACCC ACTGCCCCAG CCGCTGCCTG GGACTGAACC CACTTGGACT
CCTGGCCCTG CCCACAGTGA CTTCCTCCAG AAGATGGACG ACTTCTGGCT GCTGAAGGAG
CTGCAGACCT GGCTGTGGCG CTCGGCCAAG GACTTCAACC GGCTCAAGAA GAAGATGCAG
CCTCCAGCAG CTGCAGTCAC CCTGCACCTG GGGGCTCATG GCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin share a conserved binding site on the ciliary neurotrophic factor receptor alpha chain
J. Biol. Chem. 283 (44), 30341-30350 (2008)
Rousseau F, Chevalier S, Guillet C, Ravon E, Diveu C, Froger J, Barbier F, Grimaud L and Gascan H.


book

Mutations in cytokine receptor-like factor 1 (CRLF1) account for both Crisponi and cold-induced sweating syndromes
Am. J. Hum. Genet. 80 (5), 966-970 (2007)
Dagoneau N, Bellais S, Blanchet P, Sarda P, Al-Gazali LI, Di Rocco M, Huber C, Djouadi F, Le Goff C, Munnich A and Cormier-Daire V.


book

Inactivation of cardiotrophin-like cytokine, a second ligand for ciliary neurotrophic factor receptor, leads to cold-induced sweating syndrome in a patient
Proc. Natl. Acad. Sci. U.S.A. 103 (26), 10068-10073 (2006)
Rousseau F, Gauchat JF, McLeod JG, Chevalier S, Guillet C, Guilhot F, Cognet I, Froger J, Hahn AF, Knappskog PM, Gascan H and Boman H.


book

Transcriptome analysis of human gastric cancer
Mamm. Genome 16 (12), 942-954 (2005)
Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS and Kim NS.


book

Two different contact sites are recruited by cardiotrophin-like cytokine (CLC) to generate the CLC/CLF and CLC/sCNTFRalpha composite cytokines
J. Biol. Chem. 279 (42), 43961-43970 (2004)
Perret D, Guillet C, Elson G, Froger J, Plun-Favreau H, Rousseau F, Chabbert M, Gauchat JF and Gascan H.


book

The ciliary neurotrophic factor receptor alpha component induces the secretion of and is required for functional responses to cardiotrophin-like cytokine
EMBO J. 20 (7), 1692-1703 (2001)
Plun-Favreau H, Elson G, Chabbert M, Froger J, deLapeyriere O, Lelievre E, Guillet C, Hermann J, Gauchat JF, Gascan H and Chevalier S.


book

CLF associates with CLC to form a functional heteromeric ligand for the CNTF receptor complex
Nat. Neurosci. 3 (9), 867-872 (2000)
Elson GC, Lelievre E, Guillet C, Chevalier S, Plun-Favreau H, Froger J, Suard I, de Coignac AB, Delneste Y, Bonnefoy JY, Gauchat JF and Gascan H.


book

Novel neurotrophin-1/B cell-stimulating factor-3: a cytokine of the IL-6 family
Proc. Natl. Acad. Sci. U.S.A. 96 (20), 11458-11463 (1999)
Senaldi G, Varnum BC, Sarmiento U, Starnes C, Lile J, Scully S, Guo J, Elliott G, McNinch J, Shaklee CL, Freeman D, Manu F, Simonet WS, Boone T and Chang MS.


book

Computational EST database analysis identifies a novel member of the neuropoietic cytokine family
Biochem. Biophys. Res. Commun. 262 (1), 132-138 (1999)
Shi Y, Wang W, Yourey PA, Gohari S, Zukauskas D, Zhang J, Ruben S and Alderson RF.


book

Cold-Induced Sweating Syndrome including Crisponi Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Hahn,A.F. and Boman,H.


 
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