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ALPL cDNA ORF clone, Homo sapiens (human)

Gene Symbol ALPL
Entrez Gene ID 249
Full Name alkaline phosphatase, liver/bone/kidney
Synonyms AP-TNAP, APTNAP, HOPS, TNAP, TNSALP
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.12

Summary There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]. lac of sum
Disorder MIM:

171760

Disorder Html: Hypophosphatasia, infantile, 241500 (3); Hypophosphatasia, childhood,

mRNA and Protein(s)

mRNA Protein Name
XM_006710546 XP_006710609 alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_005245818 XP_005245875 alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_005245820 XP_005245877 alkaline phosphatase, tissue-nonspecific isozyme isoform X2
NM_001127501 NP_001120973 alkaline phosphatase, tissue-nonspecific isozyme isoform 2
NM_001177520 NP_001170991 alkaline phosphatase, tissue-nonspecific isozyme isoform 3
NM_000478 NP_000469 alkaline phosphatase, tissue-nonspecific isozyme isoform 1 precursor



Homo sapiens (human) ALPL NP_000469.3
Pan troglodytes (chimpanzee) ALPL XP_003307915.1
Macaca mulatta (Rhesus monkey) ALPL XP_001109717.2
Canis lupus familiaris (dog) ALPL NP_001184066.1
Bos taurus (cattle) ALPL NP_789828.2
Mus musculus (house mouse) Alpl NP_001274101.1
Rattus norvegicus (Norway rat) Alpl NP_037191.1
Gallus gallus (chicken) ALPL NP_990691.1
Danio rerio (zebrafish) alp3 NP_001265768.1
Drosophila melanogaster (fruit fly) CG8147 NP_649897.4
Xenopus (Silurana) tropicalis (western clawed frog) LOC100485467 XP_002943485.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu33310 XM_006710546 PREDICTED: Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu33310 XM_005245818 PREDICTED: Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu33311 XM_005245820 PREDICTED: Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22555 NM_001127501 Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu23043 NM_001177520 Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu23066 NM_000478 Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu33310
Clone ID Related Accession (Same CDS sequence) XM_005245818 , XM_006710546
Accession Version XM_006710546.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006710609.1
CDS220..1794
Misc Feature(1)355..1689(+)
Misc Feature(2)355..1665(+)
Misc Feature(3)397..1581(+)
Translation

Target ORF information:

RefSeq Version XM_006710546
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006710546

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu33310
Clone ID Related Accession (Same CDS sequence) XM_005245818 , XM_006710546
Accession Version XM_005245818.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005245875.1
CDS235..1809
Misc Feature(1)370..1704(+)
Misc Feature(2)370..1680(+)
Misc Feature(3)412..1596(+)
Translation

Target ORF information:

RefSeq Version XM_005245818
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005245818

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu33311
Clone ID Related Accession (Same CDS sequence) XM_005245820
Accession Version XM_005245820.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530360993. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGATTTCT CCCCTTTGGT
AGTCTTTCTT GGGGACCTGG CCCTGAAGGG GCAAGCAGCC CCTCAGCCTC ACTGCCCAGA
AAAGCAAATC CGCAGGCCCC GGCTTTCCCA CGCTGTGTGC TGATGTTCCC AGGCTCTTTC
AGCGCCGGCC AGGGGCCTTG CAGACAGACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005245877.1
CDS197..1369
Misc Feature(1)332..>1201(+)
Misc Feature(2)332..637(+)
Misc Feature(3)374..1192(+)
Translation

Target ORF information:

RefSeq Version XM_005245820
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005245820

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGT GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGATTTCT CCCCTTTGGT
AGTCTTTCTT GGGGACCTGG CCCTGAAGGG GCAAGCAGCC CCTCAGCCTC ACTGCCCAGA
AAAGCAAATC CGCAGGCCCC GGCTTTCCCA CGCTGTGTGC TGATGTTCCC AGGCTCTTTC
AGCGCCGGCC AGGGGCCTTG CAGACAGACC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22555
Clone ID Related Accession (Same CDS sequence) NM_001127501
Accession Version NM_001127501.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN425768.1, DA574668.1, AK298085.1, BC021289.2 and AL359815.26. On Apr 13, 2010 this sequence version replaced gi:188595717. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298085.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTTCCTGG GAGATGGGAT GGGTGTCTCC ACAGTGACGG CTGCCCGCAT CCTCAAGGGT 
CAGCTCCACC ACAACCCTGG GGAGGAGACC AGGCTGGAGA TGGACAAGTT CCCCTTCGTG
GCCCTCTCCA AGACGTACAA CACCAATGCC CAGGTCCCTG ACAGCGCCGG CACCGCCACC
GCCTACCTGT GTGGGGTGAA GGCCAATGAG GGCACCGTGG GGGTAAGCGC AGCCACTGAG
CGTTCCCGGT GCAACACCAC CCAGGGGAAC GAGGTCACCT CCATCCTGCG CTGGGCCAAG
GACGCTGGGA AATCTGTGGG CATTGTGACC ACCACGAGAG TGAACCATGC CACCCCCAGC
GCCGCCTACG CCCACTCGGC TGACCGGGAC TGGTACTCAG ACAACGAGAT GCCCCCTGAG
GCCTTGAGCC AGGGCTGTAA GGACATCGCC TACCAGCTCA TGCATAACAT CAGGGACATT
GACGTGATCA TGGGGGGTGG CCGGAAATAC ATGTACCCCA AGAATAAAAC TGATGTGGAG
TATGAGAGTG ACGAGAAAGC CAGGGGCACG AGGCTGGACG GCCTGGACCT CGTTGACACC
TGGAAGAGCT TCAAACCGAG ATACAAGCAC TCCCACTTCA TCTGGAACCG CACGGAACTC
CTGACCCTTG ACCCCCACAA TGTGGACTAC CTATTGGGTC TCTTCGAGCC AGGGGACATG
CAGTACGAGC TGAACAGGAA CAACGTGACG GACCCGTCAC TCTCCGAGAT GGTGGTGGTG
GCCATCCAGA TCCTGCGGAA GAACCCCAAA GGCTTCTTCT TGCTGGTGGA AGGAGGCAGA
ATTGACCACG GGCACCATGA AGGAAAAGCC AAGCAGGCCC TGCATGAGGC GGTGGAGATG
GACCGGGCCA TCGGGCAGGC AGGCAGCTTG ACCTCCTCGG AAGACACTCT GACCGTGGTC
ACTGCGGACC ATTCCCACGT CTTCACATTT GGTGGATACA CCCCCCGTGG CAACTCTATC
TTTGGTCTGG CCCCCATGCT GAGTGACACA GACAAGAAGC CCTTCACTGC CATCCTGTAT
GGCAATGGGC CTGGCTACAA GGTGGTGGGC GGTGAACGAG AGAATGTCTC CATGGTGGAC
TATGCTCACA ACAACTACCA GGCGCAGTCT GCTGTGCCCC TGCGCCACGA GACCCACGGC
GGGGAGGACG TGGCCGTCTT CTCCAAGGGC CCCATGGCGC ACCTGCTGCA CGGCGTCCAC
GAGCAGAACT ACGTCCCCCA CGTGATGGCG TATGCAGCCT GCATCGGGGC CAACCTCGGC
CACTGTGCTC CTGCCAGCTC GGCAGGCAGC CTTGCTGCAG GCCCCCTGCT GCTCGCGCTG
GCCCTCTACC CCCTGAGCGT CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120973.2
CDS258..1667
Misc Feature(1)258..1562(+)
Misc Feature(2)270..1454(+)
Misc Feature(3)282..1538(+)
Exon (1)1..153
Gene:ALPL
Gene Synonym:
Exon (2)154..273
Gene:ALPL
Gene Synonym:
Exon (3)274..389
Gene:ALPL
Gene Synonym:
Exon (4)390..564
Gene:ALPL
Gene Synonym:
Exon (5)565..740
Gene:ALPL
Gene Synonym:
Exon (6)741..884
Gene:ALPL
Gene Synonym:
Exon (7)885..954
Gene:ALPL
Gene Synonym:
Exon (8)955..1089
Gene:ALPL
Gene Synonym:
Exon (9)1090..1281
Gene:ALPL
Gene Synonym:
Exon (10)1282..1401
Gene:ALPL
Gene Synonym:
Exon (11)1402..2431
Gene:ALPL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127501
Organism Homo sapiens (human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127501

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTTCCTGG GAGATGGGAT GGGTGTCTCC ACAGTGACGG CTGCCCGCAT CCTCAAGGGT 
CAGCTCCACC ACAACCCTGG GGAGGAGACC AGGCTGGAGA TGGACAAGTT CCCCTTCGTG
GCCCTCTCCA AGACGTACAA CACCAATGCC CAGGTCCCTG ACAGCGCCGG CACCGCCACC
GCCTACCTGT GTGGGGTGAA GGCCAATGAG GGCACCGTGG GGGTAAGCGC AGCCACTGAG
CGTTCCCGGT GCAACACCAC CCAGGGGAAC GAGGTCACCT CCATCCTGCG CTGGGCCAAG
GACGCTGGGA AATCTGTGGG CATTGTGACC ACCACGAGAG TGAACCATGC CACCCCCAGC
GCCGCCTACG CCCACTCGGC TGACCGGGAC TGGTACTCAG ACAACGAGAT GCCCCCTGAG
GCCTTGAGCC AGGGCTGTAA GGACATCGCC TACCAGCTCA TGCATAACAT CAGGGACATT
GACGTGATCA TGGGGGGTGG CCGGAAATAC ATGTACCCCA AGAATAAAAC TGATGTGGAG
TATGAGAGTG ACGAGAAAGC CAGGGGCACG AGGCTGGACG GCCTGGACCT CGTTGACACC
TGGAAGAGCT TCAAACCGAG ATACAAGCAC TCCCACTTCA TCTGGAACCG CACGGAACTC
CTGACCCTTG ACCCCCACAA TGTGGACTAC CTATTGGGTC TCTTCGAGCC AGGGGACATG
CAGTACGAGC TGAACAGGAA CAACGTGACG GACCCGTCAC TCTCCGAGAT GGTGGTGGTG
GCCATCCAGA TCCTGCGGAA GAACCCCAAA GGCTTCTTCT TGCTGGTGGA AGGAGGCAGA
ATTGACCACG GGCACCATGA AGGAAAAGCC AAGCAGGCCC TGCATGAGGC GGTGGAGATG
GACCGGGCCA TCGGGCAGGC AGGCAGCTTG ACCTCCTCGG AAGACACTCT GACCGTGGTC
ACTGCGGACC ATTCCCACGT CTTCACATTT GGTGGATACA CCCCCCGTGG CAACTCTATC
TTTGGTCTGG CCCCCATGCT GAGTGACACA GACAAGAAGC CCTTCACTGC CATCCTGTAT
GGCAATGGGC CTGGCTACAA GGTGGTGGGC GGTGAACGAG AGAATGTCTC CATGGTGGAC
TATGCTCACA ACAACTACCA GGCGCAGTCT GCTGTGCCCC TGCGCCACGA GACCCACGGC
GGGGAGGACG TGGCCGTCTT CTCCAAGGGC CCCATGGCGC ACCTGCTGCA CGGCGTCCAC
GAGCAGAACT ACGTCCCCCA CGTGATGGCG TATGCAGCCT GCATCGGGGC CAACCTCGGC
CACTGTGCTC CTGCCAGCTC GGCAGGCAGC CTTGCTGCAG GCCCCCTGCT GCTCGCGCTG
GCCCTCTACC CCCTGAGCGT CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23043
Clone ID Related Accession (Same CDS sequence) NM_001177520
Accession Version NM_001177520.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1344bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN425768.1, DA574668.1, AK295608.1, BC021289.2 and AL359815.26. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant also lacks an exon in the 5' coding region, compared to variant 1. The encoded isoform (3) has a unique N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2159764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCCTGGA GCTTCAGAAG CTCAACACCA ACGTGGCTAA GAATGTCATC ATGTTCCTGG 
GAGATGACGT ACAACACCAA TGCCCAGGTC CCTGACAGCG CCGGCACCGC CACCGCCTAC
CTGTGTGGGG TGAAGGCCAA TGAGGGCACC GTGGGGGTAA GCGCAGCCAC TGAGCGTTCC
CGGTGCAACA CCACCCAGGG GAACGAGGTC ACCTCCATCC TGCGCTGGGC CAAGGACGCT
GGGAAATCTG TGGGCATTGT GACCACCACG AGAGTGAACC ATGCCACCCC CAGCGCCGCC
TACGCCCACT CGGCTGACCG GGACTGGTAC TCAGACAACG AGATGCCCCC TGAGGCCTTG
AGCCAGGGCT GTAAGGACAT CGCCTACCAG CTCATGCATA ACATCAGGGA CATTGACGTG
ATCATGGGGG GTGGCCGGAA ATACATGTAC CCCAAGAATA AAACTGATGT GGAGTATGAG
AGTGACGAGA AAGCCAGGGG CACGAGGCTG GACGGCCTGG ACCTCGTTGA CACCTGGAAG
AGCTTCAAAC CGAGATACAA GCACTCCCAC TTCATCTGGA ACCGCACGGA ACTCCTGACC
CTTGACCCCC ACAATGTGGA CTACCTATTG GGTCTCTTCG AGCCAGGGGA CATGCAGTAC
GAGCTGAACA GGAACAACGT GACGGACCCG TCACTCTCCG AGATGGTGGT GGTGGCCATC
CAGATCCTGC GGAAGAACCC CAAAGGCTTC TTCTTGCTGG TGGAAGGAGG CAGAATTGAC
CACGGGCACC ATGAAGGAAA AGCCAAGCAG GCCCTGCATG AGGCGGTGGA GATGGACCGG
GCCATCGGGC AGGCAGGCAG CTTGACCTCC TCGGAAGACA CTCTGACCGT GGTCACTGCG
GACCATTCCC ACGTCTTCAC ATTTGGTGGA TACACCCCCC GTGGCAACTC TATCTTTGGT
CTGGCCCCCA TGCTGAGTGA CACAGACAAG AAGCCCTTCA CTGCCATCCT GTATGGCAAT
GGGCCTGGCT ACAAGGTGGT GGGCGGTGAA CGAGAGAATG TCTCCATGGT GGACTATGCT
CACAACAACT ACCAGGCGCA GTCTGCTGTG CCCCTGCGCC ACGAGACCCA CGGCGGGGAG
GACGTGGCCG TCTTCTCCAA GGGCCCCATG GCGCACCTGC TGCACGGCGT CCACGAGCAG
AACTACGTCC CCCACGTGAT GGCGTATGCA GCCTGCATCG GGGCCAACCT CGGCCACTGT
GCTCCTGCCA GCTCGGCAGG CAGCCTTGCT GCAGGCCCCC TGCTGCTCGC GCTGGCCCTC
TACCCCCTGA GCGTCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170991.1
CDS208..1551
Misc Feature(1)202..204(+)
Misc Feature(2)271..1446(+)
Misc Feature(3)271..1422(+)
Misc Feature(4)301..1338(+)
Exon (1)1..153
Gene:ALPL
Gene Synonym:
Exon (2)154..273
Gene:ALPL
Gene Synonym:
Exon (3)274..448
Gene:ALPL
Gene Synonym:
Exon (4)449..624
Gene:ALPL
Gene Synonym:
Exon (5)625..768
Gene:ALPL
Gene Synonym:
Exon (6)769..838
Gene:ALPL
Gene Synonym:
Exon (7)839..973
Gene:ALPL
Gene Synonym:
Exon (8)974..1165
Gene:ALPL
Gene Synonym:
Exon (9)1166..1285
Gene:ALPL
Gene Synonym:
Exon (10)1286..2315
Gene:ALPL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177520
Organism Homo sapiens (human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177520

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCCTGGA GCTTCAGAAG CTCAACACCA ACGTGGCTAA GAATGTCATC ATGTTCCTGG 
GAGATGACGT ACAACACCAA TGCCCAGGTC CCTGACAGCG CCGGCACCGC CACCGCCTAC
CTGTGTGGGG TGAAGGCCAA TGAGGGCACC GTGGGGGTAA GCGCAGCCAC TGAGCGTTCC
CGGTGCAACA CCACCCAGGG GAACGAGGTC ACCTCCATCC TGCGCTGGGC CAAGGACGCT
GGGAAATCTG TGGGCATTGT GACCACCACG AGAGTGAACC ATGCCACCCC CAGCGCCGCC
TACGCCCACT CGGCTGACCG GGACTGGTAC TCAGACAACG AGATGCCCCC TGAGGCCTTG
AGCCAGGGCT GTAAGGACAT CGCCTACCAG CTCATGCATA ACATCAGGGA CATTGACGTG
ATCATGGGGG GTGGCCGGAA ATACATGTAC CCCAAGAATA AAACTGATGT GGAGTATGAG
AGTGACGAGA AAGCCAGGGG CACGAGGCTG GACGGCCTGG ACCTCGTTGA CACCTGGAAG
AGCTTCAAAC CGAGATACAA GCACTCCCAC TTCATCTGGA ACCGCACGGA ACTCCTGACC
CTTGACCCCC ACAATGTGGA CTACCTATTG GGTCTCTTCG AGCCAGGGGA CATGCAGTAC
GAGCTGAACA GGAACAACGT GACGGACCCG TCACTCTCCG AGATGGTGGT GGTGGCCATC
CAGATCCTGC GGAAGAACCC CAAAGGCTTC TTCTTGCTGG TGGAAGGAGG CAGAATTGAC
CACGGGCACC ATGAAGGAAA AGCCAAGCAG GCCCTGCATG AGGCGGTGGA GATGGACCGG
GCCATCGGGC AGGCAGGCAG CTTGACCTCC TCGGAAGACA CTCTGACCGT GGTCACTGCG
GACCATTCCC ACGTCTTCAC ATTTGGTGGA TACACCCCCC GTGGCAACTC TATCTTTGGT
CTGGCCCCCA TGCTGAGTGA CACAGACAAG AAGCCCTTCA CTGCCATCCT GTATGGCAAT
GGGCCTGGCT ACAAGGTGGT GGGCGGTGAA CGAGAGAATG TCTCCATGGT GGACTATGCT
CACAACAACT ACCAGGCGCA GTCTGCTGTG CCCCTGCGCC ACGAGACCCA CGGCGGGGAG
GACGTGGCCG TCTTCTCCAA GGGCCCCATG GCGCACCTGC TGCACGGCGT CCACGAGCAG
AACTACGTCC CCCACGTGAT GGCGTATGCA GCCTGCATCG GGGCCAACCT CGGCCACTGT
GCTCCTGCCA GCTCGGCAGG CAGCCTTGCT GCAGGCCCCC TGCTGCTCGC GCTGGCCCTC
TACCCCCTGA GCGTCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23066
Clone ID Related Accession (Same CDS sequence) NM_000478
Accession Version NM_000478.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline phosphatase, tissue-nonspecific isozyme isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN425768.1, DA574668.1, BC021289.2 and AL359815.26. This sequence is a reference standard in the RefSeqGene project. On Apr 15, 2010 this sequence version replaced gi:116734716. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021289.2, AB209814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGC GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000469.3
CDS258..1832
Misc Feature(1)393..1727(+)
Misc Feature(2)393..1703(+)
Misc Feature(3)435..1619(+)
Exon (1)1..153
Gene:ALPL
Gene Synonym:
Exon (2)154..318
Gene:ALPL
Gene Synonym:
Exon (3)319..438
Gene:ALPL
Gene Synonym:
Exon (4)439..554
Gene:ALPL
Gene Synonym:
Exon (5)555..729
Gene:ALPL
Gene Synonym:
Exon (6)730..905
Gene:ALPL
Gene Synonym:
Exon (7)906..1049
Gene:ALPL
Gene Synonym:
Exon (8)1050..1119
Gene:ALPL
Gene Synonym:
Exon (9)1120..1254
Gene:ALPL
Gene Synonym:
Exon (10)1255..1446
Gene:ALPL
Gene Synonym:
Exon (11)1447..1566
Gene:ALPL
Gene Synonym:
Exon (12)1567..2596
Gene:ALPL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000478
Organism Homo sapiens (human)
Definition Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000478

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATTTCAC CATTCTTAGT ACTGGCCATT GGCACCTGCC TTACTAACTC CTTAGTGCCA 
GAGAAAGAGA AAGACCCCAA GTACTGGCGA GACCAAGCGC AAGAGACACT GAAATATGCC
CTGGAGCTTC AGAAGCTCAA CACCAACGTG GCTAAGAATG TCATCATGTT CCTGGGAGAT
GGGATGGGTG TCTCCACAGT GACGGCTGCC CGCATCCTCA AGGGTCAGCT CCACCACAAC
CCTGGGGAGG AGACCAGGCT GGAGATGGAC AAGTTCCCCT TCGTGGCCCT CTCCAAGACG
TACAACACCA ATGCCCAGGT CCCTGACAGC GCCGGCACCG CCACCGCCTA CCTGTGTGGG
GTGAAGGCCA ATGAGGGCAC CGTGGGGGTA AGCGCAGCCA CTGAGCGTTC CCGGTGCAAC
ACCACCCAGG GGAACGAGGT CACCTCCATC CTGCGCTGGG CCAAGGACGC TGGGAAATCT
GTGGGCATTG TGACCACCAC GAGAGTGAAC CATGCCACCC CCAGCGCCGC CTACGCCCAC
TCGGCTGACC GGGACTGGTA CTCAGACAAC GAGATGCCCC CTGAGGCCTT GAGCCAGGGC
TGTAAGGACA TCGCCTACCA GCTCATGCAT AACATCAGGG ACATTGACGT GATCATGGGG
GGTGGCCGGA AATACATGTA CCCCAAGAAT AAAACTGATG TGGAGTATGA GAGTGACGAG
AAAGCCAGGG GCACGAGGCT GGACGGCCTG GACCTCGTTG ACACCTGGAA GAGCTTCAAA
CCGAGATACA AGCACTCCCA CTTCATCTGG AACCGCACGG AACTCCTGAC CCTTGACCCC
CACAATGTGG ACTACCTATT GGGTCTCTTC GAGCCAGGGG ACATGCAGTA CGAGCTGAAC
AGGAACAACG TGACGGACCC GTCACTCTCC GAGATGGTGG TGGTGGCCAT CCAGATCCTG
CGGAAGAACC CCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGAATTGA CCACGGGCAC
CATGAAGGAA AAGCCAAGCA GGCCCTGCAT GAGGCGGTGG AGATGGACCG GGCCATCGGG
CAGGCAGGCA GCTTGACCTC CTCGGAAGAC ACTCTGACCG TGGTCACTGC GGACCATTCC
CACGTCTTCA CATTTGGTGG ATACACCCCC CGTGGCAACT CTATCTTTGG TCTGGCCCCC
ATGCTGAGTG ACACAGACAA GAAGCCCTTC ACTGCCATCC TGTATGGCAA TGGGCCTGGC
TACAAGGTGG TGGGCGGTGA ACGAGAGAAT GTCTCCATGG TGGACTATGC TCACAACAAC
TACCAGGCGC AGTCTGCTGT GCCCCTGCGC CACGAGACCC ACGGCGGGGA GGACGTGGCC
GTCTTCTCCA AGGGCCCCAT GGCGCACCTG CTGCACGGCG TCCACGAGCA GAACTACGTC
CCCCACGTGA TGGCGTATGC AGCCTGCATC GGGGCCAACC TCGGCCACTG TGCTCCTGCC
AGCTCGGCAG GCAGCCTTGC TGCAGGCCCC CTGCTGCTCG CGCTGGCCCT CTACCCCCTG
AGCGTCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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