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FNDC5 fibronectin type III domain containing 5 [Homo sapiens (human)]

Gene Symbol FNDC5
Entrez Gene ID 252995
Full Name fibronectin type III domain containing 5
Synonyms FRCP2, irisin
General protein information
Preferred Names
fibronectin type III domain-containing protein 5
Names
fibronectin type III domain-containing protein 5
fibronectin type III repeat-containing protein 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p35.1

Summary This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. lac of sum
Disorder MIM:

611906

Disorder Html:





The following FNDC5 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FNDC5 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20539 NM_001171940 Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $99
OHu22617 NM_001171941 Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu27675 NM_153756 Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu20539
Accession Version NM_001171940.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 546bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-MAY-2014
Organism Homo sapiens (human)
Product fibronectin type III domain-containing protein 5 isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE502835.1, BX537781.1 and BC033123.1. Summary: This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. Transcript Variant: This variant (3) contains multiple differences in the UTRs and coding region, compared to variant 1. It initiates translation from an alternate 'AUA' start codon that is conserved as an 'AUG' start codon in orthologs. The encoded isoform (3) is longer and has distinct N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537781.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATTGAA
GCGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001165411.2
CDS68..613
Misc Feature(1)167..439(+)
Misc Feature(2)167..403(+)
Misc Feature(3)404..418(+)
Misc Feature(4)524..586(+)
Exon (1)1..161
Gene:FNDC5
Gene Synonym:
Exon (2)162..277
Gene:FNDC5
Gene Synonym:
Exon (3)278..476
Gene:FNDC5
Gene Synonym:
Exon (4)477..566
Gene:FNDC5
Gene Synonym:
Exon (5)567..598
Gene:FNDC5
Gene Synonym:
Exon (6)599..2083
Gene:FNDC5
Gene Synonym:
Translation
Position Chain Variation Link
27 27 a, c dbSNP:191621656
28 28 c, t dbSNP:187247333
184 184 a, c dbSNP:55783359
191 191 a, g dbSNP:41265873
257 257 a, g dbSNP:750342894
281 281 -, aag dbSNP:775897042
281 281 a, g dbSNP:368098828
288 288 a, t dbSNP:777659320
291 291 a, g dbSNP:373917435
296 296 a, c dbSNP:747854483
297 297 c, t dbSNP:370511876
300 300 a, g dbSNP:140092865
305 305 a, c dbSNP:375151828
314 314 a, g dbSNP:376090198
318 318 a, c, g dbSNP:758944873
324 324 c, t dbSNP:374085063
327 327 c, t dbSNP:765656287
340 340 c, t dbSNP:149496621
363 363 c, t dbSNP:754092673
364 364 a, g, t dbSNP:371060777
367 367 -, g dbSNP:772354615
372 372 c, t dbSNP:532898911
379 379 c, t dbSNP:772243605
380 380 a, g dbSNP:762976068
418 418 c, t dbSNP:377691602
419 419 a, g dbSNP:769640098
442 442 a, g dbSNP:201963189
443 443 a, c dbSNP:780955257
444 444 a, g dbSNP:369474610
447 447 a, g dbSNP:768347509
449 449 a, g dbSNP:746635434
451 451 c, t dbSNP:779570641
454 454 c, g dbSNP:757797906
462 462 c, t dbSNP:746399642
463 463 c, t dbSNP:779220112
479 479 a, g dbSNP:745434931
481 481 c, g dbSNP:778471724
483 483 -, t dbSNP:749367886
490 490 c, g dbSNP:757750681
491 491 a, g dbSNP:749779922
510 510 a, c dbSNP:370205345
516 516 c, t dbSNP:377741902
519 519 a, g, t dbSNP:137888693
526 526 c, t dbSNP:755217107
533 533 c, t dbSNP:138727728
535 535 a, g dbSNP:766452920
538 538 c, t dbSNP:761689081
539 539 -, atc dbSNP:777889321
541 541 c, t dbSNP:776502849
542 542 a, g dbSNP:189231805
544 544 c, t dbSNP:763891316
545 545 a, g dbSNP:200824057
547 547 c, g dbSNP:775260802
557 557 a, g dbSNP:771678427
559 559 a, g dbSNP:745510759
564 564 c, t dbSNP:773770710
573 573 c, g, t dbSNP:772839146
587 587 c, t dbSNP:770475492
588 588 a, g dbSNP:140995767
594 594 a, g dbSNP:781443548
600 600 c, t dbSNP:780070233
603 603 c, t dbSNP:772133195
614 614 c, g dbSNP:186735140
634 634 a, g dbSNP:745717447
671 671 c, t dbSNP:72882315
672 672 a, g dbSNP:570174355
742 742 c, t dbSNP:41265871
770 770 c, t dbSNP:536490448
793 793 a, c dbSNP:565862595
800 800 a, t dbSNP:559443224
835 835 a, t dbSNP:182307482
854 854 c, t dbSNP:769648870
864 864 c, t dbSNP:192675355
876 876 a, t dbSNP:748147852
896 896 c, t dbSNP:111297582
900 900 c, t dbSNP:111792806
970 970 c, t dbSNP:41265869
1057 1057 -, at dbSNP:535149739
1089 1089 a, g dbSNP:188449869
1103 1103 c, g dbSNP:750577983
1110 1110 c, g dbSNP:374096384
1115 1115 c, t dbSNP:559498677
1177 1177 a, t dbSNP:550848772
1197 1197 -, t dbSNP:148383625
1202 1202 g, t dbSNP:541586637
1252 1252 a, g dbSNP:779155435
1286 1286 a, g dbSNP:113655390
1302 1302 c, t dbSNP:757574640
1330 1330 c, t dbSNP:559166695
1340 1340 a, c dbSNP:753912370
1350 1350 c, t dbSNP:764274681
1364 1364 a, g dbSNP:780382746
1379 1379 a, g dbSNP:372376939
1396 1396 c, t dbSNP:761260388
1397 1397 a, g dbSNP:753316197
1400 1400 c, t dbSNP:543458021
1418 1418 c, t dbSNP:576494283
1456 1456 c, t dbSNP:767753578
1464 1464 a, c, g dbSNP:74583158
1490 1490 c, t dbSNP:183238750
1542 1542 -, tc dbSNP:138570533
1599 1599 c, t dbSNP:566148395
1610 1610 c, t dbSNP:773220232
1611 1611 a, g dbSNP:774613701
1618 1618 a, g dbSNP:770573110
1653 1653 a, c dbSNP:191897980
1655 1655 c, g dbSNP:538785458
1660 1660 c, t dbSNP:187740130
1665 1665 c, t dbSNP:550044166
1685 1685 c, t dbSNP:531247443
1738 1738 a, g dbSNP:146223915
1749 1749 a, g dbSNP:548697855
1766 1766 a, g dbSNP:183460978
1787 1787 c, t dbSNP:3480
1789 1789 a, g dbSNP:372054809
1794 1794 c, t dbSNP:141940806
1795 1795 a, g dbSNP:769349233
1804 1804 g, t dbSNP:532694562
1820 1820 c, t dbSNP:565488826
1828 1828 a, g dbSNP:748053787
1844 1844 c, g dbSNP:578201136
2006 2006 a, g dbSNP:544110773

Target ORF information:

RefSeq Version NM_001171940
Organism Homo sapiens (human)
Definition Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001171940

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATTGAA
GCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22617
Accession Version NM_001171941.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 462bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibronectin type III domain-containing protein 5 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CV815003.1, AC114493.3, BC062297.1, DA393478.1 and BC033123.1. On Jun 8, 2013 this sequence version replaced gi:288541421. Summary: This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. Transcript Variant: This variant (1) represents the longest transcript and encodes the shortest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062297.1, BU151187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGCTGCGCT TCATCCAGGA GGTGAACACC ACCACCCGCT CATGTGCCCT CTGGGACCTG 
GAGGAGGATA CGGAGTACAT AGTCCACGTG CAGGCCATCT CCATTCAGGG CCAGAGCCCA
GCCAGCGAGC CTGTGCTCTT CAAGACCCCG CGTGAGGCTG AGAAGATGGC CTCCAAGAAC
AAAGATGAGG TAACCATGAA AGAGATGGGG AGGAACCAAC AGCTGCGGAC AGGCGAGGTG
CTGATCATCG TCGTGGTCCT GTTCATGTGG GCAGGTGTCA TTGCCCTCTT CTGCCGCCAG
TATGACATCA TCAAGGACAA TGAACCCAAT AACAACAAGG AAAAAACCAA GAGTGCATCA
GAAACCAGCA CACCAGAGCA CCAGGGCGGG GGGCTTCTCC GCAGCAAGGT GAGGGCAAGA
CCTGGGCCTG GGTGGGCCAC CCTGTGCCTC ATGCTCTGGT AA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001165412.1
CDS227..688
Misc Feature(1)80..82(+)
Misc Feature(2)239..373(+)
Misc Feature(3)290..337(+)
Misc Feature(4)338..352(+)
Exon (1)1..99
Gene:FNDC5
Gene Synonym:
Exon (2)100..211
Gene:FNDC5
Gene Synonym:
Exon (3)212..410
Gene:FNDC5
Gene Synonym:
Exon (4)411..500
Gene:FNDC5
Gene Synonym:
Exon (5)501..3030
Gene:FNDC5
Gene Synonym:
Translation
Position Chain Variation Link
32 32 -, ag dbSNP:528154237
52 52 c, t dbSNP:563605105
87 87 c, g dbSNP:542023424
118 118 a, c dbSNP:55783359
125 125 a, g dbSNP:41265873
191 191 a, g dbSNP:750342894
215 215 -, aag dbSNP:775897042
215 215 a, g dbSNP:368098828
222 222 a, t dbSNP:777659320
225 225 a, g dbSNP:373917435
230 230 a, c dbSNP:747854483
231 231 c, t dbSNP:370511876
234 234 a, g dbSNP:140092865
239 239 a, c dbSNP:375151828
248 248 a, g dbSNP:376090198
252 252 a, c, g dbSNP:758944873
258 258 c, t dbSNP:374085063
261 261 c, t dbSNP:765656287
274 274 c, t dbSNP:149496621
297 297 c, t dbSNP:754092673
298 298 a, g, t dbSNP:371060777
301 301 -, g dbSNP:772354615
306 306 c, t dbSNP:532898911
313 313 c, t dbSNP:772243605
314 314 a, g dbSNP:762976068
352 352 c, t dbSNP:377691602
353 353 a, g dbSNP:769640098
376 376 a, g dbSNP:201963189
377 377 a, c dbSNP:780955257
378 378 a, g dbSNP:369474610
381 381 a, g dbSNP:768347509
383 383 a, g dbSNP:746635434
385 385 c, t dbSNP:779570641
388 388 c, g dbSNP:757797906
396 396 c, t dbSNP:746399642
397 397 c, t dbSNP:779220112
413 413 a, g dbSNP:745434931
415 415 c, g dbSNP:778471724
417 417 -, t dbSNP:749367886
424 424 c, g dbSNP:757750681
425 425 a, g dbSNP:749779922
444 444 a, c dbSNP:370205345
450 450 c, t dbSNP:377741902
453 453 a, g, t dbSNP:137888693
460 460 c, t dbSNP:755217107
467 467 c, t dbSNP:138727728
469 469 a, g dbSNP:766452920
472 472 c, t dbSNP:761689081
473 473 -, atc dbSNP:777889321
475 475 c, t dbSNP:776502849
476 476 a, g dbSNP:189231805
478 478 c, t dbSNP:763891316
479 479 a, g dbSNP:200824057
481 481 c, g dbSNP:775260802
491 491 a, g dbSNP:771678427
493 493 a, g dbSNP:745510759
498 498 c, t dbSNP:773770710
507 507 c, g, t dbSNP:772839146
521 521 c, t dbSNP:770475492
522 522 a, g dbSNP:140995767
528 528 a, g dbSNP:781443548
553 553 c, g dbSNP:769018609
555 555 a, g dbSNP:143697125
562 562 c, g dbSNP:202111610
564 564 a, t dbSNP:780257477
573 573 c, t dbSNP:758613897
576 576 a, g dbSNP:750559821
579 579 a, g dbSNP:557154418
584 584 c, t dbSNP:756083445
597 597 c, t dbSNP:185141197
600 600 a, c dbSNP:767234279
602 602 c, g dbSNP:761991187
605 605 a, c dbSNP:759315666
613 613 c, t dbSNP:145770830
614 614 a, c, g dbSNP:377098383
627 627 a, g dbSNP:144593537
629 629 a, g dbSNP:769391902
636 636 c, t dbSNP:762433406
637 637 a, g dbSNP:777138875
639 639 g, t dbSNP:768908833
645 645 c, g dbSNP:755006302
652 652 a, g dbSNP:775703433
656 656 a, c, g dbSNP:372666011
673 673 c, t dbSNP:778991988
676 676 c, t dbSNP:757275309
680 680 c, t dbSNP:748046461
682 682 c, g dbSNP:781114267
684 684 c, g dbSNP:754861512
685 685 a, g dbSNP:751422783
686 686 -, t dbSNP:754767987
690 690 c, t dbSNP:766250108
710 710 c, g dbSNP:758159093
715 715 c, t dbSNP:369180960
716 716 a, c, g dbSNP:776566391
717 717 c, g dbSNP:535065879
723 723 a, g dbSNP:764719478
727 727 c, t dbSNP:761061713
730 730 c, t dbSNP:775962780
781 781 c, t dbSNP:570749928
842 842 a, g dbSNP:200858488
843 843 -, aa dbSNP:79030802
843 843 a, g dbSNP:376693329
866 866 c, g dbSNP:533304419
890 890 c, t dbSNP:776881064
892 892 c, t dbSNP:537131281
910 910 a, g dbSNP:372506396
951 951 -, tt dbSNP:751253414
953 953 -, gc dbSNP:765983669
959 959 a, g dbSNP:772384825
960 960 c, g dbSNP:746107867
966 966 c, t dbSNP:570139154
967 967 a, g dbSNP:368841129
968 968 c, t dbSNP:747249835
972 972 a, g dbSNP:749448885
976 976 g, t dbSNP:777695590
978 978 -, atttc dbSNP:749959474
980 980 c, t dbSNP:754986054
985 985 c, t dbSNP:746885246
993 993 a, g dbSNP:375525627
999 999 a, g dbSNP:758210235
1000 1000 c, t dbSNP:200027292
1013 1013 c, t dbSNP:765051347
1016 1016 a, g dbSNP:756847854
1019 1019 -, c dbSNP:764721095
1024 1024 c, g dbSNP:753436209
1035 1035 a, g dbSNP:529875998
1037 1037 c, g dbSNP:761275467
1038 1038 a, c dbSNP:201474569
1041 1041 a, t dbSNP:767877282
1042 1042 c, g dbSNP:759827284
1043 1043 a, g dbSNP:375515075
1044 1044 a, g dbSNP:771237329
1046 1046 c, g dbSNP:534383288
1047 1047 a, g dbSNP:763056953
1055 1055 -, a dbSNP:565086016
1055 1055 a, c dbSNP:773376587
1056 1056 g, t dbSNP:371600008
1099 1099 a, t dbSNP:559730294
1130 1130 c, t dbSNP:777456142
1143 1143 c, t dbSNP:114049350
1163 1163 g, t dbSNP:532165087
1173 1173 c, t dbSNP:564701915
1179 1179 a, g dbSNP:111531850
1270 1270 a, g dbSNP:759099386
1303 1303 g, t dbSNP:772077083
1330 1330 c, t dbSNP:145617804
1369 1369 a, g dbSNP:190199568
1434 1434 c, t dbSNP:771066279
1436 1436 c, g dbSNP:184741588
1467 1467 a, c dbSNP:774105238
1474 1474 -, g dbSNP:551370813
1479 1479 c, t dbSNP:149745698
1489 1489 c, g dbSNP:574690918
1504 1504 -, tc dbSNP:761173102
1513 1513 a, c, t dbSNP:191148874
1514 1514 c, t dbSNP:577196941
1547 1547 c, t dbSNP:780070233
1550 1550 c, t dbSNP:772133195
1561 1561 c, g dbSNP:186735140
1581 1581 a, g dbSNP:745717447
1618 1618 c, t dbSNP:72882315
1619 1619 a, g dbSNP:570174355
1689 1689 c, t dbSNP:41265871
1717 1717 c, t dbSNP:536490448
1740 1740 a, c dbSNP:565862595
1747 1747 a, t dbSNP:559443224
1782 1782 a, t dbSNP:182307482
1801 1801 c, t dbSNP:769648870
1811 1811 c, t dbSNP:192675355
1823 1823 a, t dbSNP:748147852
1843 1843 c, t dbSNP:111297582
1847 1847 c, t dbSNP:111792806
1917 1917 c, t dbSNP:41265869
2004 2004 -, at dbSNP:535149739
2036 2036 a, g dbSNP:188449869
2050 2050 c, g dbSNP:750577983
2057 2057 c, g dbSNP:374096384
2062 2062 c, t dbSNP:559498677
2124 2124 a, t dbSNP:550848772
2144 2144 -, t dbSNP:148383625
2149 2149 g, t dbSNP:541586637
2199 2199 a, g dbSNP:779155435
2233 2233 a, g dbSNP:113655390
2249 2249 c, t dbSNP:757574640
2277 2277 c, t dbSNP:559166695
2287 2287 a, c dbSNP:753912370
2297 2297 c, t dbSNP:764274681
2311 2311 a, g dbSNP:780382746
2326 2326 a, g dbSNP:372376939
2343 2343 c, t dbSNP:761260388
2344 2344 a, g dbSNP:753316197
2347 2347 c, t dbSNP:543458021
2365 2365 c, t dbSNP:576494283
2403 2403 c, t dbSNP:767753578
2411 2411 a, c, g dbSNP:74583158
2437 2437 c, t dbSNP:183238750
2489 2489 -, tc dbSNP:138570533
2546 2546 c, t dbSNP:566148395
2557 2557 c, t dbSNP:773220232
2558 2558 a, g dbSNP:774613701
2565 2565 a, g dbSNP:770573110
2600 2600 a, c dbSNP:191897980
2602 2602 c, g dbSNP:538785458
2607 2607 c, t dbSNP:187740130
2612 2612 c, t dbSNP:550044166
2632 2632 c, t dbSNP:531247443
2685 2685 a, g dbSNP:146223915
2696 2696 a, g dbSNP:548697855
2713 2713 a, g dbSNP:183460978
2734 2734 c, t dbSNP:3480
2736 2736 a, g dbSNP:372054809
2741 2741 c, t dbSNP:141940806
2742 2742 a, g dbSNP:769349233
2751 2751 g, t dbSNP:532694562
2767 2767 c, t dbSNP:565488826
2775 2775 a, g dbSNP:748053787
2791 2791 c, g dbSNP:578201136
2953 2953 a, g dbSNP:544110773

Target ORF information:

RefSeq Version NM_001171941
Organism Homo sapiens (human)
Definition Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001171941

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCTGCGCT TCATCCAGGA GGTGAACACC ACCACCCGCT CATGTGCCCT CTGGGACCTG 
GAGGAGGATA CGGAGTACAT AGTCCACGTG CAGGCCATCT CCATTCAGGG CCAGAGCCCA
GCCAGCGAGC CTGTGCTCTT CAAGACCCCG CGTGAGGCTG AGAAGATGGC CTCCAAGAAC
AAAGATGAGG TAACCATGAA AGAGATGGGG AGGAACCAAC AGCTGCGGAC AGGCGAGGTG
CTGATCATCG TCGTGGTCCT GTTCATGTGG GCAGGTGTCA TTGCCCTCTT CTGCCGCCAG
TATGACATCA TCAAGGACAA TGAACCCAAT AACAACAAGG AAAAAACCAA GAGTGCATCA
GAAACCAGCA CACCAGAGCA CCAGGGCGGG GGGCTTCTCC GCAGCAAGGT GAGGGCAAGA
CCTGGGCCTG GGTGGGCCAC CCTGTGCCTC ATGCTCTGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27675
Accession Version NM_153756.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibronectin type III domain-containing protein 5 isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE502835.1, AK092102.1 and BC033123.1. On Feb 5, 2010 this sequence version replaced gi:24371259. Summary: This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. Transcript Variant: This variant (2) contains multiple differences in the UTRs and coding region, compared to variant 1. It initiates translation from an alternate 'AUA' start codon that is conserved as an 'AUG' start codon in orthologs. The encoded isoform (2) is longer and has distinct N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK092102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATCAAG
GACAATGAAC CCAATAACAA CAAGGAAAAA ACCAAGAGTG CATCAGAAAC CAGCACACCA
GAGCACCAGG GCGGGGGGCT TCTCCGCAGC AAGATATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_715637.2
CDS68..706
Misc Feature(1)167..439(+)
Misc Feature(2)167..403(+)
Misc Feature(3)404..418(+)
Exon (1)1..161
Gene:FNDC5
Gene Synonym:
Exon (2)162..277
Gene:FNDC5
Gene Synonym:
Exon (3)278..476
Gene:FNDC5
Gene Synonym:
Exon (4)477..566
Gene:FNDC5
Gene Synonym:
Exon (5)567..700
Gene:FNDC5
Gene Synonym:
Exon (6)701..2732
Gene:FNDC5
Gene Synonym:
Translation
Position Chain Variation Link
27 27 a, c dbSNP:191621656
28 28 c, t dbSNP:187247333
184 184 a, c dbSNP:55783359
191 191 a, g dbSNP:41265873
257 257 a, g dbSNP:750342894
281 281 -, aag dbSNP:775897042
281 281 a, g dbSNP:368098828
288 288 a, t dbSNP:777659320
291 291 a, g dbSNP:373917435
296 296 a, c dbSNP:747854483
297 297 c, t dbSNP:370511876
300 300 a, g dbSNP:140092865
305 305 a, c dbSNP:375151828
314 314 a, g dbSNP:376090198
318 318 a, c, g dbSNP:758944873
324 324 c, t dbSNP:374085063
327 327 c, t dbSNP:765656287
340 340 c, t dbSNP:149496621
363 363 c, t dbSNP:754092673
364 364 a, g, t dbSNP:371060777
367 367 -, g dbSNP:772354615
372 372 c, t dbSNP:532898911
379 379 c, t dbSNP:772243605
380 380 a, g dbSNP:762976068
418 418 c, t dbSNP:377691602
419 419 a, g dbSNP:769640098
442 442 a, g dbSNP:201963189
443 443 a, c dbSNP:780955257
444 444 a, g dbSNP:369474610
447 447 a, g dbSNP:768347509
449 449 a, g dbSNP:746635434
451 451 c, t dbSNP:779570641
454 454 c, g dbSNP:757797906
462 462 c, t dbSNP:746399642
463 463 c, t dbSNP:779220112
479 479 a, g dbSNP:745434931
481 481 c, g dbSNP:778471724
483 483 -, t dbSNP:749367886
490 490 c, g dbSNP:757750681
491 491 a, g dbSNP:749779922
510 510 a, c dbSNP:370205345
516 516 c, t dbSNP:377741902
519 519 a, g, t dbSNP:137888693
526 526 c, t dbSNP:755217107
533 533 c, t dbSNP:138727728
535 535 a, g dbSNP:766452920
538 538 c, t dbSNP:761689081
539 539 -, atc dbSNP:777889321
541 541 c, t dbSNP:776502849
542 542 a, g dbSNP:189231805
544 544 c, t dbSNP:763891316
545 545 a, g dbSNP:200824057
547 547 c, g dbSNP:775260802
557 557 a, g dbSNP:771678427
559 559 a, g dbSNP:745510759
564 564 c, t dbSNP:773770710
573 573 c, g, t dbSNP:772839146
587 587 c, t dbSNP:770475492
588 588 a, g dbSNP:140995767
594 594 a, g dbSNP:781443548
619 619 c, g dbSNP:769018609
621 621 a, g dbSNP:143697125
628 628 c, g dbSNP:202111610
630 630 a, t dbSNP:780257477
639 639 c, t dbSNP:758613897
642 642 a, g dbSNP:750559821
645 645 a, g dbSNP:557154418
650 650 c, t dbSNP:756083445
663 663 c, t dbSNP:185141197
666 666 a, c dbSNP:767234279
668 668 c, g dbSNP:761991187
671 671 a, c dbSNP:759315666
679 679 c, t dbSNP:145770830
680 680 a, c, g dbSNP:377098383
693 693 a, g dbSNP:144593537
695 695 a, g dbSNP:769391902
701 701 a, g dbSNP:758210235
702 702 c, t dbSNP:200027292
715 715 c, t dbSNP:765051347
718 718 a, g dbSNP:756847854
721 721 -, c dbSNP:764721095
726 726 c, g dbSNP:753436209
737 737 a, g dbSNP:529875998
739 739 c, g dbSNP:761275467
740 740 a, c dbSNP:201474569
743 743 a, t dbSNP:767877282
744 744 c, g dbSNP:759827284
745 745 a, g dbSNP:375515075
746 746 a, g dbSNP:771237329
748 748 c, g dbSNP:534383288
749 749 a, g dbSNP:763056953
757 757 -, a dbSNP:565086016
757 757 a, c dbSNP:773376587
758 758 g, t dbSNP:371600008
801 801 a, t dbSNP:559730294
832 832 c, t dbSNP:777456142
845 845 c, t dbSNP:114049350
865 865 g, t dbSNP:532165087
875 875 c, t dbSNP:564701915
881 881 a, g dbSNP:111531850
972 972 a, g dbSNP:759099386
1005 1005 g, t dbSNP:772077083
1032 1032 c, t dbSNP:145617804
1071 1071 a, g dbSNP:190199568
1136 1136 c, t dbSNP:771066279
1138 1138 c, g dbSNP:184741588
1169 1169 a, c dbSNP:774105238
1176 1176 -, g dbSNP:551370813
1181 1181 c, t dbSNP:149745698
1191 1191 c, g dbSNP:574690918
1206 1206 -, tc dbSNP:761173102
1215 1215 a, c, t dbSNP:191148874
1216 1216 c, t dbSNP:577196941
1249 1249 c, t dbSNP:780070233
1252 1252 c, t dbSNP:772133195
1263 1263 c, g dbSNP:186735140
1283 1283 a, g dbSNP:745717447
1320 1320 c, t dbSNP:72882315
1321 1321 a, g dbSNP:570174355
1391 1391 c, t dbSNP:41265871
1419 1419 c, t dbSNP:536490448
1442 1442 a, c dbSNP:565862595
1449 1449 a, t dbSNP:559443224
1484 1484 a, t dbSNP:182307482
1503 1503 c, t dbSNP:769648870
1513 1513 c, t dbSNP:192675355
1525 1525 a, t dbSNP:748147852
1545 1545 c, t dbSNP:111297582
1549 1549 c, t dbSNP:111792806
1619 1619 c, t dbSNP:41265869
1706 1706 -, at dbSNP:535149739
1738 1738 a, g dbSNP:188449869
1752 1752 c, g dbSNP:750577983
1759 1759 c, g dbSNP:374096384
1764 1764 c, t dbSNP:559498677
1826 1826 a, t dbSNP:550848772
1846 1846 -, t dbSNP:148383625
1851 1851 g, t dbSNP:541586637
1901 1901 a, g dbSNP:779155435
1935 1935 a, g dbSNP:113655390
1951 1951 c, t dbSNP:757574640
1979 1979 c, t dbSNP:559166695
1989 1989 a, c dbSNP:753912370
1999 1999 c, t dbSNP:764274681
2013 2013 a, g dbSNP:780382746
2028 2028 a, g dbSNP:372376939
2045 2045 c, t dbSNP:761260388
2046 2046 a, g dbSNP:753316197
2049 2049 c, t dbSNP:543458021
2067 2067 c, t dbSNP:576494283
2105 2105 c, t dbSNP:767753578
2113 2113 a, c, g dbSNP:74583158
2139 2139 c, t dbSNP:183238750
2191 2191 -, tc dbSNP:138570533
2248 2248 c, t dbSNP:566148395
2259 2259 c, t dbSNP:773220232
2260 2260 a, g dbSNP:774613701
2267 2267 a, g dbSNP:770573110
2302 2302 a, c dbSNP:191897980
2304 2304 c, g dbSNP:538785458
2309 2309 c, t dbSNP:187740130
2314 2314 c, t dbSNP:550044166
2334 2334 c, t dbSNP:531247443
2387 2387 a, g dbSNP:146223915
2398 2398 a, g dbSNP:548697855
2415 2415 a, g dbSNP:183460978
2436 2436 c, t dbSNP:3480
2438 2438 a, g dbSNP:372054809
2443 2443 c, t dbSNP:141940806
2444 2444 a, g dbSNP:769349233
2453 2453 g, t dbSNP:532694562
2469 2469 c, t dbSNP:565488826
2477 2477 a, g dbSNP:748053787
2493 2493 c, g dbSNP:578201136
2655 2655 a, g dbSNP:544110773

Target ORF information:

RefSeq Version NM_153756
Organism Homo sapiens (human)
Definition Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153756

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATCAAG
GACAATGAAC CCAATAACAA CAAGGAAAAA ACCAAGAGTG CATCAGAAAC CAGCACACCA
GAGCACCAGG GCGGGGGGCT TCTCCGCAGC AAGATATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.