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FNDC5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol FNDC5
Entrez Gene ID 252995
Full Name fibronectin type III domain containing 5
Synonyms FRCP2, irisin
General protein information
Preferred Names
fibronectin type III domain-containing protein 5
Names
fibronectin type III domain-containing protein 5
fibronectin type III repeat-containing protein 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p35.1

Summary This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]. lac of sum
Disorder MIM:

611906


mRNA and Protein(s)

mRNA Protein Name
NM_001171940 NP_001165411 fibronectin type III domain-containing protein 5 isoform 3 preproprotein
NM_001171941 NP_001165412 fibronectin type III domain-containing protein 5 isoform 1
NM_153756 NP_715637 fibronectin type III domain-containing protein 5 isoform 2 preproprotein


Homo sapiens (human) FNDC5 NP_715637.2
Macaca mulatta (Rhesus monkey) LOC710159 XP_001098747.2
Canis lupus familiaris (dog) FNDC5 XP_005617677.1
Mus musculus (house mouse) Fndc5 NP_081678.1
Rattus norvegicus (Norway rat) Fndc5 NP_001257910.1
Gallus gallus (chicken) FNDC5 XP_417814.4
Danio rerio (zebrafish) fndc5b NP_001037802.1
Danio rerio (zebrafish) fndc5a XP_001335404.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FNDC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FNDC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20539 NM_001171940 Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
OHu22617 NM_001171941 Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu27675 NM_153756 Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20539
Clone ID Related Accession (Same CDS sequence) NM_001171940
Accession Version NM_001171940.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 546bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-MAY-2014
Organism Homo sapiens (human)
Product fibronectin type III domain-containing protein 5 isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE502835.1, BX537781.1 and BC033123.1. Transcript Variant: This variant (3) contains multiple differences in the UTRs and coding region, compared to variant 1. It initiates translation from an alternate 'AUA' start codon that is conserved as an 'AUG' start codon in orthologs. The encoded isoform (3) is longer and has distinct N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537781.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATTGAA
GCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001165411.2
CDS68..613
Misc Feature(1)167..439(+)
Misc Feature(2)167..403(+)
Misc Feature(3)404..418(+)
Misc Feature(4)524..586(+)
Exon (1)1..161
Gene:FNDC5
Gene Synonym:
Exon (2)162..277
Gene:FNDC5
Gene Synonym:
Exon (3)278..476
Gene:FNDC5
Gene Synonym:
Exon (4)477..566
Gene:FNDC5
Gene Synonym:
Exon (5)567..598
Gene:FNDC5
Gene Synonym:
Exon (6)599..2083
Gene:FNDC5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001171940
Organism Homo sapiens (human)
Definition Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001171940

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATTGAA
GCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22617
Clone ID Related Accession (Same CDS sequence) NM_001171941
Accession Version NM_001171941.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 462bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibronectin type III domain-containing protein 5 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CV815003.1, AC114493.3, BC062297.1, DA393478.1 and BC033123.1. On Jun 8, 2013 this sequence version replaced gi:288541421. Transcript Variant: This variant (1) represents the longest transcript and encodes the shortest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062297.1, BU151187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGCTGCGCT TCATCCAGGA GGTGAACACC ACCACCCGCT CATGTGCCCT CTGGGACCTG 
GAGGAGGATA CGGAGTACAT AGTCCACGTG CAGGCCATCT CCATTCAGGG CCAGAGCCCA
GCCAGCGAGC CTGTGCTCTT CAAGACCCCG CGTGAGGCTG AGAAGATGGC CTCCAAGAAC
AAAGATGAGG TAACCATGAA AGAGATGGGG AGGAACCAAC AGCTGCGGAC AGGCGAGGTG
CTGATCATCG TCGTGGTCCT GTTCATGTGG GCAGGTGTCA TTGCCCTCTT CTGCCGCCAG
TATGACATCA TCAAGGACAA TGAACCCAAT AACAACAAGG AAAAAACCAA GAGTGCATCA
GAAACCAGCA CACCAGAGCA CCAGGGCGGG GGGCTTCTCC GCAGCAAGGT GAGGGCAAGA
CCTGGGCCTG GGTGGGCCAC CCTGTGCCTC ATGCTCTGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001165412.1
CDS227..688
Misc Feature(1)80..82(+)
Misc Feature(2)239..373(+)
Misc Feature(3)290..337(+)
Misc Feature(4)338..352(+)
Exon (1)1..99
Gene:FNDC5
Gene Synonym:
Exon (2)100..211
Gene:FNDC5
Gene Synonym:
Exon (3)212..410
Gene:FNDC5
Gene Synonym:
Exon (4)411..500
Gene:FNDC5
Gene Synonym:
Exon (5)501..3030
Gene:FNDC5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001171941
Organism Homo sapiens (human)
Definition Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001171941

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCTGCGCT TCATCCAGGA GGTGAACACC ACCACCCGCT CATGTGCCCT CTGGGACCTG 
GAGGAGGATA CGGAGTACAT AGTCCACGTG CAGGCCATCT CCATTCAGGG CCAGAGCCCA
GCCAGCGAGC CTGTGCTCTT CAAGACCCCG CGTGAGGCTG AGAAGATGGC CTCCAAGAAC
AAAGATGAGG TAACCATGAA AGAGATGGGG AGGAACCAAC AGCTGCGGAC AGGCGAGGTG
CTGATCATCG TCGTGGTCCT GTTCATGTGG GCAGGTGTCA TTGCCCTCTT CTGCCGCCAG
TATGACATCA TCAAGGACAA TGAACCCAAT AACAACAAGG AAAAAACCAA GAGTGCATCA
GAAACCAGCA CACCAGAGCA CCAGGGCGGG GGGCTTCTCC GCAGCAAGGT GAGGGCAAGA
CCTGGGCCTG GGTGGGCCAC CCTGTGCCTC ATGCTCTGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27675
Clone ID Related Accession (Same CDS sequence) NM_153756
Accession Version NM_153756.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fibronectin type III domain-containing protein 5 isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE502835.1, AK092102.1 and BC033123.1. On Feb 5, 2010 this sequence version replaced gi:24371259. Transcript Variant: This variant (2) contains multiple differences in the UTRs and coding region, compared to variant 1. It initiates translation from an alternate 'AUA' start codon that is conserved as an 'AUG' start codon in orthologs. The encoded isoform (2) is longer and has distinct N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK092102.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATCAAG
GACAATGAAC CCAATAACAA CAAGGAAAAA ACCAAGAGTG CATCAGAAAC CAGCACACCA
GAGCACCAGG GCGGGGGGCT TCTCCGCAGC AAGATATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_715637.2
CDS68..706
Misc Feature(1)167..439(+)
Misc Feature(2)167..403(+)
Misc Feature(3)404..418(+)
Exon (1)1..161
Gene:FNDC5
Gene Synonym:
Exon (2)162..277
Gene:FNDC5
Gene Synonym:
Exon (3)278..476
Gene:FNDC5
Gene Synonym:
Exon (4)477..566
Gene:FNDC5
Gene Synonym:
Exon (5)567..700
Gene:FNDC5
Gene Synonym:
Exon (6)701..2732
Gene:FNDC5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_153756
Organism Homo sapiens (human)
Definition Homo sapiens fibronectin type III domain containing 5 (FNDC5), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153756

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATACACCCCG GGTCGCCGAG CGCCTGGCCG CCCCGCGCCC GCGCCGCGCT CCGCCTGTGG 
CTGGGCTGCG TCTGCTTCGC GCTGGTGCAG GCGGACAGTC CCTCAGCCCC AGTGAACGTC
ACCGTCAGGC ACCTCAAGGC CAACTCTGCA GTGGTGAGCT GGGATGTTCT GGAGGATGAG
GTTGTCATCG GATTTGCCAT CTCCCAGCAG AAGAAGGATG TGCGGATGCT GCGCTTCATC
CAGGAGGTGA ACACCACCAC CCGCTCATGT GCCCTCTGGG ACCTGGAGGA GGATACGGAG
TACATAGTCC ACGTGCAGGC CATCTCCATT CAGGGCCAGA GCCCAGCCAG CGAGCCTGTG
CTCTTCAAGA CCCCGCGTGA GGCTGAGAAG ATGGCCTCCA AGAACAAAGA TGAGGTAACC
ATGAAAGAGA TGGGGAGGAA CCAACAGCTG CGGACAGGCG AGGTGCTGAT CATCGTCGTG
GTCCTGTTCA TGTGGGCAGG TGTCATTGCC CTCTTCTGCC GCCAGTATGA CATCATCAAG
GACAATGAAC CCAATAACAA CAAGGAAAAA ACCAAGAGTG CATCAGAAAC CAGCACACCA
GAGCACCAGG GCGGGGGGCT TCTCCGCAGC AAGATATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Serum levels of the myokine irisin in relation to metabolic and renal function
Eur. J. Endocrinol. 170 (4), 501-506 (2014)
Ebert T, Focke D, Petroff D, Wurst U, Richter J, Bachmann A, Lossner U, Kralisch S, Kratzsch J, Beige J, Bast I, Anders M, Bluher M, Stumvoll M and Fasshauer M.


book

The identification of irisin in human cerebrospinal fluid: influence of adiposity, metabolic markers, and gestational diabetes
Am. J. Physiol. Endocrinol. Metab. 306 (5), E512-E518 (2014)
Piya MK, Harte AL, Sivakumar K, Tripathi G, Voyias PD, James S, Sabico S, Al-Daghri NM, Saravanan P, Barber TM, Kumar S, Vatish M and McTernan PG.


book

Irisin ERKs the fat
Diabetes 63 (2), 381-383 (2014)
Wu J and Spiegelman BM.


book

The effects of acute and chronic exercise on PGC-1alpha, irisin and browning of subcutaneous adipose tissue in humans
FEBS J. 281 (3), 739-749 (2014)
Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, Gulseth HL, Birkeland KI, Jensen J and Drevon CA.


book

Expression of the irisin precursor FNDC5 in skeletal muscle correlates with aerobic exercise performance in patients with heart failure
Circ Heart Fail 5 (6), 812-818 (2012)
Lecker SH, Zavin A, Cao P, Arena R, Allsup K, Daniels KM, Joseph J, Schulze PC and Forman DE.


book

Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human
Cell 150 (2), 366-376 (2012)
Wu J, Bostrom P, Sparks LM, Ye L, Choi JH, Giang AH, Khandekar M, Virtanen KA, Nuutila P, Schaart G, Huang K, Tu H, van Marken Lichtenbelt WD, Hoeks J, Enerback S, Schrauwen P and Spiegelman BM.


book

Medicine. Irisin, light my fire
Science 336 (6077), 42-43 (2012)
Kelly DP.


book

A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis
Nature 481 (7382), 463-468 (2012)
Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostrom EA, Choi JH, Long JZ, Kajimura S, Zingaretti MC, Vind BF, Tu H, Cinti S, Hojlund K, Gygi SP and Spiegelman BM.


book

Identification of evolutionarily conserved non-AUG-initiated N-terminal extensions in human coding sequences
Nucleic Acids Res. 39 (10), 4220-4234 (2011)
Ivanov IP, Firth AE, Michel AM, Atkins JF and Baranov PV.


book

Frcp1 and Frcp2, two novel fibronectin type III repeat containing genes
Gene 297 (1-2), 79-83 (2002)
Teufel A, Malik N, Mukhopadhyay M and Westphal H.


 
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