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G6PC glucose-6-phosphatase, catalytic subunit [Homo sapiens (human)]

Gene Symbol G6PC
Entrez Gene ID 2538
Full Name glucose-6-phosphatase, catalytic subunit
Synonyms G6PC1, G6PT, GSD1, GSD1a
General protein information
Preferred Names
glucose-6-phosphatase
Names
glucose-6-phosphatase
G6Pase
G-6-Pase
G6Pase-alpha
glucose-6-phosphatase alpha
NP_000142.1
NP_000142.2
NP_001257326.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21

Summary Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

613742

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu20803 NM_000151 Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu21715 NM_001270397 Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu20803D
Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucose-6-phosphatase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, U01120.1, AC016889.28 and AW614228.1. On Jul 3, 2012 this sequence version replaced gi:119393889. Summary: Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: U01120.1, BC130478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_000142.2
CDS81..1154
Misc Feature(1)60..62(+)
Misc Feature(2)165..227(+)
Misc Feature(3)195..656(+)
Misc Feature(4)204..920(+)
Misc Feature(5)261..323(+)
Misc Feature(6)306..620(+)
Misc Feature(7)432..494(+)
Misc Feature(8)522..584(+)
Misc Feature(9)618..686(+)
Misc Feature(10)708..770(+)
Misc Feature(11)843..905(+)
Misc Feature(12)954..1016(+)
Misc Feature(13)1041..1103(+)
Exon (1)1..310
Gene:G6PC
Gene Synonym:
Exon (2)311..420
Gene:G6PC
Gene Synonym:
Exon (3)421..526
Gene:G6PC
Gene Synonym:
Exon (4)527..642
Gene:G6PC
Gene Synonym:
Exon (5)643..4161
Gene:G6PC
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:145752565
36 36 a, g dbSNP:367816430
41 41 c, g dbSNP:751555417
42 42 c, g dbSNP:756696261
58 58 c, t dbSNP:201423586
60 60 a, t dbSNP:750000403
62 62 a, g dbSNP:571324259
63 63 a, g dbSNP:779355487
66 66 g, t dbSNP:373065173
72 72 -, a dbSNP:34960677
79 79 a, g dbSNP:371618834
82 82 c, t dbSNP:778406722
83 83 a, g dbSNP:587780945
95 95 a, c, g dbSNP:374766396
97 97 a, g dbSNP:776795347
98 98 c, t dbSNP:144652516
99 99 a, g dbSNP:758837069
106 106 a, g dbSNP:770007378
115 115 g, t dbSNP:775755718
118 118 c, t dbSNP:762723628
122 122 a, g, t dbSNP:763922040
126 126 a, g dbSNP:761839506
146 146 c, t dbSNP:766886771
155 155 c, g dbSNP:369488416
157 157 -, c dbSNP:768209865
159 159 -, c dbSNP:80356479
161 161 a, c, g dbSNP:371611000
162 162 a, g dbSNP:750711451
164 164 c, t dbSNP:758804611
172 172 a, t dbSNP:538960604
178 178 a, t dbSNP:778314834
182 182 c, t dbSNP:747426958
183 183 a, g dbSNP:757798234
188 188 c, t dbSNP:148461633
189 189 a, c, g dbSNP:781064575
193 193 a, t dbSNP:104894565
198 198 a, g dbSNP:376555092
207 207 c, t dbSNP:775667677
212 212 c, t dbSNP:202190197
213 213 a, g dbSNP:145749644
216 216 c, t dbSNP:570514299
255 255 a, g dbSNP:774215572
259 259 a, g dbSNP:761595049
264 264 c, t dbSNP:767487454
269 269 a, g dbSNP:764920787
273 273 g, t dbSNP:369472089
288 288 c, t dbSNP:766030585
295 295 a, g dbSNP:753502076
299 299 a, c, t dbSNP:373545775
300 300 a, g dbSNP:751959283
303 303 g, t dbSNP:757628083
305 305 c, t dbSNP:781714941
309 309 c, t dbSNP:104894566
319 319 c, t dbSNP:750886274
321 321 c, g dbSNP:756632286
322 322 a, g dbSNP:780047423
327 327 c, t dbSNP:1801175
328 328 a, g dbSNP:1801176
329 329 c, t dbSNP:779176162
332 332 a, g dbSNP:148967047
335 335 c, t dbSNP:748363083
338 338 a, g dbSNP:142917638
340 340 -, g dbSNP:755612674
349 349 a, g dbSNP:771864998
374 374 c, t dbSNP:777452219
375 375 a, g dbSNP:746885993
384 384 a, g, t dbSNP:375219700
391 391 a, t dbSNP:759101908
400 400 a, t dbSNP:769235934
408 408 a, g dbSNP:104894567
411 411 a, t dbSNP:762539496
421 421 a, g dbSNP:544563908
425 425 c, t dbSNP:774055151
432 432 a, g dbSNP:761007323
434 434 c, g dbSNP:766771010
437 437 c, t dbSNP:562533427
445 445 a, g dbSNP:759982943
448 448 c, t dbSNP:765306500
450 450 a, g dbSNP:104894568
454 454 a, g dbSNP:752726795
456 456 -, ta dbSNP:756356652
459 459 -, at dbSNP:606231368
459 459 c, t dbSNP:532763969
460 460 -, ta dbSNP:80356488
464 464 c, t dbSNP:764401169
465 465 a, g dbSNP:751875988
469 469 c, t dbSNP:757083891
512 512 a, g dbSNP:161628
515 515 c, g dbSNP:745770086
518 518 -, t dbSNP:777888691
519 519 a, g dbSNP:756018092
525 525 c, t dbSNP:779548820
526 526 a, g dbSNP:181624619
527 527 a, g dbSNP:549712764
530 530 c, t dbSNP:753856233
533 533 a, g dbSNP:369892096
538 538 c, t dbSNP:778417581
539 539 c, t dbSNP:747740866
551 551 a, g dbSNP:771720935
577 577 g, t dbSNP:104894571
580 580 a, g dbSNP:777552825
588 588 c, t dbSNP:373345919
589 589 a, g dbSNP:750470654
598 598 c, t dbSNP:775826449
612 612 c, g dbSNP:763543607
613 613 c, t dbSNP:768803329
614 614 a, c, t dbSNP:774585761
627 627 a, g dbSNP:767846924
629 629 c, g, t dbSNP:375921716
631 631 a, g dbSNP:104894569
633 633 a, g dbSNP:766230068
638 638 a, g, t dbSNP:141376085
639 639 c, t dbSNP:200905457
642 642 a, c, g dbSNP:80356482
643 643 a, g dbSNP:760981149
644 644 c, t dbSNP:766030987
650 650 a, t dbSNP:753671536
662 662 c, t dbSNP:754345839
664 664 c, t dbSNP:77814737
665 665 a, c, g dbSNP:765150017
690 690 g, t dbSNP:201961848
695 695 c, t dbSNP:374522795
704 704 a, c dbSNP:751120985
714 714 a, g dbSNP:756944737
718 718 c, t dbSNP:201649392
723 723 c, t dbSNP:780212338
726 726 c, t dbSNP:749665480
728 728 g, t dbSNP:80356484
731 731 c, t dbSNP:779538234
737 737 c, t dbSNP:201520075
738 738 a, g dbSNP:771997505
739 739 c, t dbSNP:773356062
743 743 c, t dbSNP:747134498
745 745 a, c, g dbSNP:515726229
756 756 c, g dbSNP:765763631
757 757 c, t dbSNP:759306891
765 765 a, g dbSNP:765059867
774 774 a, g dbSNP:145172999
803 803 c, t dbSNP:762967975
804 804 c, t dbSNP:80356485
806 806 a, g, t dbSNP:9905900
808 808 a, g dbSNP:756784163
814 814 a, g dbSNP:767184894
815 815 c, t dbSNP:750147891
820 820 a, g dbSNP:753009862
830 830 a, g dbSNP:140869027
837 837 a, g dbSNP:559563847
848 848 a, g dbSNP:758775116
852 852 c, t dbSNP:779444123
860 860 c, t dbSNP:748610705
866 866 c, g, t dbSNP:138509513
873 873 c, g dbSNP:140224951
878 878 -, c dbSNP:749323139
880 880 a, c, t dbSNP:145296477
881 881 a, c, g dbSNP:755630386
884 884 c, t dbSNP:377077333
886 886 c, t dbSNP:769527058
889 889 g, t dbSNP:80356483
893 893 c, g dbSNP:370182849
896 896 a, g dbSNP:762728678
900 900 a, g dbSNP:764084156
901 901 c, t dbSNP:774212157
902 902 c, t dbSNP:761361643
905 905 a, c dbSNP:767174294
910 910 a, c, t dbSNP:750048146
916 916 c, t dbSNP:765631320
926 926 a, g, t dbSNP:374980545
936 936 a, g dbSNP:534670926
942 942 c, t dbSNP:776048625
945 945 a, g dbSNP:748841842
950 950 a, g dbSNP:368041878
958 958 c, t dbSNP:752049826
959 959 a, g dbSNP:529957748
963 963 c, t dbSNP:104894563
964 964 a, g dbSNP:375212414
972 972 c, t dbSNP:770003650
976 976 c, t dbSNP:780317668
989 989 c, t dbSNP:368282218
990 990 a, g dbSNP:768385469
993 993 c, t dbSNP:774311350
996 996 c, t dbSNP:554145213
1002 1002 a, c, g dbSNP:774448424
1021 1021 c, t dbSNP:771651069
1022 1022 a, c, g dbSNP:141352548
1023 1023 c, g dbSNP:143321486
1024 1024 c, t dbSNP:753486821
1028 1028 a, c, t dbSNP:137978566
1034 1034 a, c, t dbSNP:372612424
1035 1035 a, g dbSNP:781180357
1037 1037 a, g dbSNP:111259657
1040 1040 g, t dbSNP:531727073
1041 1041 a, g dbSNP:756241523
1049 1049 a, c, t dbSNP:780226142
1050 1050 a, g dbSNP:376389208
1054 1054 c, t dbSNP:778587726
1058 1058 -, ctt dbSNP:770928376
1059 1059 -, ttc dbSNP:80356486
1070 1070 c, t dbSNP:748053431
1072 1072 c, t dbSNP:149486847
1073 1073 a, c, g dbSNP:369238258
1080 1080 c, t dbSNP:770610294
1082 1082 c, t dbSNP:143991162
1084 1084 c, t dbSNP:532423062
1091 1091 c, t dbSNP:373605105
1092 1092 a, g, t dbSNP:367727229
1098 1098 -, gtc dbSNP:779105509
1102 1102 -, tcccct dbSNP:745799712
1102 1102 a, t dbSNP:387906505
1115 1115 c, t dbSNP:750975981
1118 1118 c, t dbSNP:756155679
1119 1119 c, g, t dbSNP:80356487
1131 1131 c, t dbSNP:766448695
1135 1135 c, t dbSNP:374852238
1136 1136 a, g dbSNP:779263877
1139 1139 c, g dbSNP:747942809
1143 1143 a, g dbSNP:758318402
1144 1144 a, g dbSNP:777825445
1145 1145 a, g, t dbSNP:746978011
1148 1148 -, gtt dbSNP:772035505
1148 1148 a, g dbSNP:776022311
1157 1157 a, g dbSNP:191399793
1163 1163 g, t dbSNP:566247950
1165 1165 a, g dbSNP:775267643
1167 1167 c, g dbSNP:762140129
1170 1170 c, t dbSNP:767908090
1171 1171 a, g dbSNP:34537381
1177 1177 c, t dbSNP:2229611
1178 1178 a, c dbSNP:377739870
1180 1180 a, g dbSNP:372909414
1186 1186 a, c dbSNP:755143616
1188 1188 a, g dbSNP:765355094
1190 1190 c, t dbSNP:753012050
1191 1191 a, t dbSNP:758232327
1199 1199 g, t dbSNP:537055293
1203 1203 -, gag dbSNP:775240201
1206 1206 c, g dbSNP:746889750
1227 1227 g, t dbSNP:761973914
1236 1236 c, g dbSNP:184246813
1256 1256 a, g dbSNP:577036654
1274 1274 a, g dbSNP:150477898
1289 1289 a, g dbSNP:138332426
1363 1363 a, g dbSNP:367967732
1404 1404 a, g dbSNP:143802152
1405 1405 c, t dbSNP:753206089
1410 1410 a, t dbSNP:765390240
1441 1441 c, t dbSNP:541803451
1464 1464 a, g dbSNP:563588003
1470 1470 a, g dbSNP:371213492
1521 1521 c, t dbSNP:750508510
1522 1522 g, t dbSNP:530674636
1545 1545 c, t dbSNP:543413807
1549 1549 c, t dbSNP:758748813
1603 1603 a, c dbSNP:564906976
1646 1646 a, c dbSNP:532485841
1664 1664 a, g dbSNP:547464411
1710 1710 c, t dbSNP:533491939
1746 1746 g, t dbSNP:750061113
1757 1757 c, t dbSNP:755684225
1771 1771 a, g dbSNP:146600876
1812 1812 a, g dbSNP:758483700
1819 1819 c, t dbSNP:530307111
1837 1837 c, t dbSNP:779780631
1841 1841 a, g dbSNP:748752168
1857 1857 a, g dbSNP:375295552
1858 1858 c, t dbSNP:139091543
1866 1866 a, g dbSNP:754543282
1881 1881 c, t dbSNP:80193931
1899 1899 g, t dbSNP:116897758
1951 1951 c, t dbSNP:552220012
1971 1971 c, t dbSNP:570444384
1983 1983 g, t dbSNP:149299573
2031 2031 c, t dbSNP:72826982
2043 2043 a, c dbSNP:777466548
2044 2044 a, g dbSNP:746849569
2047 2047 c, t dbSNP:754827320
2084 2084 a, c dbSNP:574584322
2099 2099 -, c dbSNP:766279304
2140 2140 a, t dbSNP:370935410
2165 2165 -, cta dbSNP:537108675
2166 2166 c, t dbSNP:75063448
2174 2174 c, t dbSNP:776851181
2184 2184 a, g dbSNP:529429768
2193 2193 g, t dbSNP:549474805
2207 2207 a, g dbSNP:556830972
2280 2280 c, t dbSNP:575186130
2359 2359 c, t dbSNP:545658086
2360 2360 -, tc dbSNP:201313100
2364 2364 c, g dbSNP:144568330
2366 2366 -, t dbSNP:36092308
2367 2367 -, c, t dbSNP:11436510
2368 2368 c, t dbSNP:200704026
2381 2381 -, t dbSNP:397735497
2448 2448 a, c dbSNP:161620
2451 2451 c, t dbSNP:559807250
2458 2458 a, g dbSNP:549833149
2478 2478 c, t dbSNP:548787621
2554 2554 a, c, g dbSNP:113536060
2587 2587 a, g dbSNP:763493186
2608 2608 c, t dbSNP:148453821
2626 2626 a, g dbSNP:572871169
2643 2643 c, t dbSNP:552879065
2665 2665 a, g dbSNP:558231884
2667 2667 c, t dbSNP:374021788
2668 2668 a, g, t dbSNP:111309126
2695 2695 a, g dbSNP:534589177
2699 2699 -, ttttagaa dbSNP:71157674
2704 2704 -, gaatttta dbSNP:777455116
2705 2705 -, aattttag dbSNP:748804734
2706 2706 a, t dbSNP:138968865
2707 2707 -, ttttagaa dbSNP:397717945
2708 2708 -, tttagaag dbSNP:201722515
2759 2759 g, t dbSNP:537388227
2794 2794 a, g, t dbSNP:556943624
2806 2806 c, t dbSNP:765883953
2811 2811 a, g dbSNP:546236128
2818 2818 a, t dbSNP:7210335
2825 2825 c, t dbSNP:568149292
2829 2829 a, c, g dbSNP:377751565
2896 2896 a, c dbSNP:571386177
2949 2949 a, g dbSNP:367885331
3017 3017 c, t dbSNP:575079591
3021 3021 g, t dbSNP:775865016
3065 3065 c, t dbSNP:539395778
3091 3091 a, t dbSNP:370221388
3121 3121 c, t dbSNP:754518597
3124 3124 a, g dbSNP:192778595
3135 3135 c, t dbSNP:573802724
3140 3140 c, t dbSNP:542411157
3217 3217 c, t dbSNP:758226811
3242 3242 a, t dbSNP:9902963
3249 3249 a, t dbSNP:692180
3253 3253 c, t dbSNP:141802218
3261 3261 a, g dbSNP:776939484
3271 3271 g, t dbSNP:544700252
3287 3287 c, t dbSNP:183542930
3318 3318 c, g dbSNP:770763503
3338 3338 a, t dbSNP:146025743
3348 3348 c, t dbSNP:563896823
3465 3465 c, t dbSNP:372226654
3470 3470 c, t dbSNP:552942446
3502 3502 g, t dbSNP:376601294
3572 3572 c, g dbSNP:115371692
3579 3579 a, t dbSNP:374104069
3628 3628 c, t dbSNP:143133684
3629 3629 a, g dbSNP:546598294
3685 3685 c, t dbSNP:562655160
3686 3686 a, g dbSNP:76156285
3743 3743 c, t dbSNP:763118529
3765 3765 c, g, t dbSNP:186556135
3780 3780 a, g dbSNP:774929925
3827 3827 -, t dbSNP:113390675
3877 3877 a, g dbSNP:146698137
3931 3931 g, t dbSNP:191738448
3940 3940 -, a dbSNP:755732323
3953 3953 c, t dbSNP:539257353
4001 4001 c, t dbSNP:139115520
4006 4006 a, g dbSNP:762083428
4069 4069 c, g, t dbSNP:566449657
4086 4086 c, t dbSNP:533168611
4111 4111 c, t dbSNP:149904153
4129 4129 a, t dbSNP:145828729
4130 4130 g, t dbSNP:753514553

Target ORF information:

RefSeq Version NM_000151
Organism Homo sapiens (human)
Definition Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_000151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu21715D
Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucose-6-phosphatase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, BC130478.1, AC016889.28 and AW614228.1. Summary: Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]. Transcript Variant: This variant (2) lacks an internal segment in the coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK303771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATTA A
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001257326.1
CDS81..611
Misc Feature(1)60..62(+)
Misc Feature(2)165..227(+)
Misc Feature(3)204..>422(+)
Misc Feature(4)261..323(+)
Exon (1)1..310
Gene:G6PC
Gene Synonym:
Exon (2)311..420
Gene:G6PC
Gene Synonym:
Exon (3)421..449
Gene:G6PC
Gene Synonym:
Exon (4)450..565
Gene:G6PC
Gene Synonym:
Exon (5)566..4084
Gene:G6PC
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:145752565
36 36 a, g dbSNP:367816430
41 41 c, g dbSNP:751555417
42 42 c, g dbSNP:756696261
58 58 c, t dbSNP:201423586
60 60 a, t dbSNP:750000403
62 62 a, g dbSNP:571324259
63 63 a, g dbSNP:779355487
66 66 g, t dbSNP:373065173
72 72 -, a dbSNP:34960677
79 79 a, g dbSNP:371618834
82 82 c, t dbSNP:778406722
83 83 a, g dbSNP:587780945
95 95 a, c, g dbSNP:374766396
97 97 a, g dbSNP:776795347
98 98 c, t dbSNP:144652516
99 99 a, g dbSNP:758837069
106 106 a, g dbSNP:770007378
115 115 g, t dbSNP:775755718
118 118 c, t dbSNP:762723628
122 122 a, g, t dbSNP:763922040
126 126 a, g dbSNP:761839506
146 146 c, t dbSNP:766886771
155 155 c, g dbSNP:369488416
157 157 -, c dbSNP:768209865
159 159 -, c dbSNP:80356479
161 161 a, c, g dbSNP:371611000
162 162 a, g dbSNP:750711451
164 164 c, t dbSNP:758804611
172 172 a, t dbSNP:538960604
178 178 a, t dbSNP:778314834
182 182 c, t dbSNP:747426958
183 183 a, g dbSNP:757798234
188 188 c, t dbSNP:148461633
189 189 a, c, g dbSNP:781064575
193 193 a, t dbSNP:104894565
198 198 a, g dbSNP:376555092
207 207 c, t dbSNP:775667677
212 212 c, t dbSNP:202190197
213 213 a, g dbSNP:145749644
216 216 c, t dbSNP:570514299
255 255 a, g dbSNP:774215572
259 259 a, g dbSNP:761595049
264 264 c, t dbSNP:767487454
269 269 a, g dbSNP:764920787
273 273 g, t dbSNP:369472089
288 288 c, t dbSNP:766030585
295 295 a, g dbSNP:753502076
299 299 a, c, t dbSNP:373545775
300 300 a, g dbSNP:751959283
303 303 g, t dbSNP:757628083
305 305 c, t dbSNP:781714941
309 309 c, t dbSNP:104894566
319 319 c, t dbSNP:750886274
321 321 c, g dbSNP:756632286
322 322 a, g dbSNP:780047423
327 327 c, t dbSNP:1801175
328 328 a, g dbSNP:1801176
329 329 c, t dbSNP:779176162
332 332 a, g dbSNP:148967047
335 335 c, t dbSNP:748363083
338 338 a, g dbSNP:142917638
340 340 -, g dbSNP:755612674
349 349 a, g dbSNP:771864998
374 374 c, t dbSNP:777452219
375 375 a, g dbSNP:746885993
384 384 a, g, t dbSNP:375219700
391 391 a, t dbSNP:759101908
400 400 a, t dbSNP:769235934
408 408 a, g dbSNP:104894567
411 411 a, t dbSNP:762539496
435 435 a, g dbSNP:161628
438 438 c, g dbSNP:745770086
441 441 -, t dbSNP:777888691
442 442 a, g dbSNP:756018092
448 448 c, t dbSNP:779548820
449 449 a, g dbSNP:181624619
450 450 a, g dbSNP:549712764
453 453 c, t dbSNP:753856233
456 456 a, g dbSNP:369892096
461 461 c, t dbSNP:778417581
462 462 c, t dbSNP:747740866
474 474 a, g dbSNP:771720935
500 500 g, t dbSNP:104894571
503 503 a, g dbSNP:777552825
511 511 c, t dbSNP:373345919
512 512 a, g dbSNP:750470654
521 521 c, t dbSNP:775826449
535 535 c, g dbSNP:763543607
536 536 c, t dbSNP:768803329
537 537 a, c, t dbSNP:774585761
550 550 a, g dbSNP:767846924
552 552 c, g, t dbSNP:375921716
554 554 a, g dbSNP:104894569
556 556 a, g dbSNP:766230068
561 561 a, g, t dbSNP:141376085
562 562 c, t dbSNP:200905457
565 565 a, c, g dbSNP:80356482
566 566 a, g dbSNP:760981149
567 567 c, t dbSNP:766030987
573 573 a, t dbSNP:753671536
585 585 c, t dbSNP:754345839
587 587 c, t dbSNP:77814737
588 588 a, c, g dbSNP:765150017
613 613 g, t dbSNP:201961848
618 618 c, t dbSNP:374522795
627 627 a, c dbSNP:751120985
637 637 a, g dbSNP:756944737
641 641 c, t dbSNP:201649392
646 646 c, t dbSNP:780212338
649 649 c, t dbSNP:749665480
651 651 g, t dbSNP:80356484
654 654 c, t dbSNP:779538234
660 660 c, t dbSNP:201520075
661 661 a, g dbSNP:771997505
662 662 c, t dbSNP:773356062
666 666 c, t dbSNP:747134498
668 668 a, c, g dbSNP:515726229
679 679 c, g dbSNP:765763631
680 680 c, t dbSNP:759306891
688 688 a, g dbSNP:765059867
697 697 a, g dbSNP:145172999
726 726 c, t dbSNP:762967975
727 727 c, t dbSNP:80356485
729 729 a, g, t dbSNP:9905900
731 731 a, g dbSNP:756784163
737 737 a, g dbSNP:767184894
738 738 c, t dbSNP:750147891
743 743 a, g dbSNP:753009862
753 753 a, g dbSNP:140869027
760 760 a, g dbSNP:559563847
771 771 a, g dbSNP:758775116
775 775 c, t dbSNP:779444123
783 783 c, t dbSNP:748610705
789 789 c, g, t dbSNP:138509513
796 796 c, g dbSNP:140224951
801 801 -, c dbSNP:749323139
803 803 a, c, t dbSNP:145296477
804 804 a, c, g dbSNP:755630386
807 807 c, t dbSNP:377077333
809 809 c, t dbSNP:769527058
812 812 g, t dbSNP:80356483
816 816 c, g dbSNP:370182849
819 819 a, g dbSNP:762728678
823 823 a, g dbSNP:764084156
824 824 c, t dbSNP:774212157
825 825 c, t dbSNP:761361643
828 828 a, c dbSNP:767174294
833 833 a, c, t dbSNP:750048146
839 839 c, t dbSNP:765631320
849 849 a, g, t dbSNP:374980545
859 859 a, g dbSNP:534670926
865 865 c, t dbSNP:776048625
868 868 a, g dbSNP:748841842
873 873 a, g dbSNP:368041878
881 881 c, t dbSNP:752049826
882 882 a, g dbSNP:529957748
886 886 c, t dbSNP:104894563
887 887 a, g dbSNP:375212414
895 895 c, t dbSNP:770003650
899 899 c, t dbSNP:780317668
912 912 c, t dbSNP:368282218
913 913 a, g dbSNP:768385469
916 916 c, t dbSNP:774311350
919 919 c, t dbSNP:554145213
925 925 a, c, g dbSNP:774448424
944 944 c, t dbSNP:771651069
945 945 a, c, g dbSNP:141352548
946 946 c, g dbSNP:143321486
947 947 c, t dbSNP:753486821
951 951 a, c, t dbSNP:137978566
957 957 a, c, t dbSNP:372612424
958 958 a, g dbSNP:781180357
960 960 a, g dbSNP:111259657
963 963 g, t dbSNP:531727073
964 964 a, g dbSNP:756241523
972 972 a, c, t dbSNP:780226142
973 973 a, g dbSNP:376389208
977 977 c, t dbSNP:778587726
981 981 -, ctt dbSNP:770928376
982 982 -, ttc dbSNP:80356486
993 993 c, t dbSNP:748053431
995 995 c, t dbSNP:149486847
996 996 a, c, g dbSNP:369238258
1003 1003 c, t dbSNP:770610294
1005 1005 c, t dbSNP:143991162
1007 1007 c, t dbSNP:532423062
1014 1014 c, t dbSNP:373605105
1015 1015 a, g, t dbSNP:367727229
1021 1021 -, gtc dbSNP:779105509
1025 1025 -, tcccct dbSNP:745799712
1025 1025 a, t dbSNP:387906505
1038 1038 c, t dbSNP:750975981
1041 1041 c, t dbSNP:756155679
1042 1042 c, g, t dbSNP:80356487
1054 1054 c, t dbSNP:766448695
1058 1058 c, t dbSNP:374852238
1059 1059 a, g dbSNP:779263877
1062 1062 c, g dbSNP:747942809
1066 1066 a, g dbSNP:758318402
1067 1067 a, g dbSNP:777825445
1068 1068 a, g, t dbSNP:746978011
1071 1071 -, gtt dbSNP:772035505
1071 1071 a, g dbSNP:776022311
1080 1080 a, g dbSNP:191399793
1086 1086 g, t dbSNP:566247950
1088 1088 a, g dbSNP:775267643
1090 1090 c, g dbSNP:762140129
1093 1093 c, t dbSNP:767908090
1094 1094 a, g dbSNP:34537381
1100 1100 c, t dbSNP:2229611
1101 1101 a, c dbSNP:377739870
1103 1103 a, g dbSNP:372909414
1109 1109 a, c dbSNP:755143616
1111 1111 a, g dbSNP:765355094
1113 1113 c, t dbSNP:753012050
1114 1114 a, t dbSNP:758232327
1122 1122 g, t dbSNP:537055293
1126 1126 -, gag dbSNP:775240201
1129 1129 c, g dbSNP:746889750
1150 1150 g, t dbSNP:761973914
1159 1159 c, g dbSNP:184246813
1179 1179 a, g dbSNP:577036654
1197 1197 a, g dbSNP:150477898
1212 1212 a, g dbSNP:138332426
1286 1286 a, g dbSNP:367967732
1327 1327 a, g dbSNP:143802152
1328 1328 c, t dbSNP:753206089
1333 1333 a, t dbSNP:765390240
1364 1364 c, t dbSNP:541803451
1387 1387 a, g dbSNP:563588003
1393 1393 a, g dbSNP:371213492
1444 1444 c, t dbSNP:750508510
1445 1445 g, t dbSNP:530674636
1468 1468 c, t dbSNP:543413807
1472 1472 c, t dbSNP:758748813
1526 1526 a, c dbSNP:564906976
1569 1569 a, c dbSNP:532485841
1587 1587 a, g dbSNP:547464411
1633 1633 c, t dbSNP:533491939
1669 1669 g, t dbSNP:750061113
1680 1680 c, t dbSNP:755684225
1694 1694 a, g dbSNP:146600876
1735 1735 a, g dbSNP:758483700
1742 1742 c, t dbSNP:530307111
1760 1760 c, t dbSNP:779780631
1764 1764 a, g dbSNP:748752168
1780 1780 a, g dbSNP:375295552
1781 1781 c, t dbSNP:139091543
1789 1789 a, g dbSNP:754543282
1804 1804 c, t dbSNP:80193931
1822 1822 g, t dbSNP:116897758
1874 1874 c, t dbSNP:552220012
1894 1894 c, t dbSNP:570444384
1906 1906 g, t dbSNP:149299573
1954 1954 c, t dbSNP:72826982
1966 1966 a, c dbSNP:777466548
1967 1967 a, g dbSNP:746849569
1970 1970 c, t dbSNP:754827320
2007 2007 a, c dbSNP:574584322
2022 2022 -, c dbSNP:766279304
2063 2063 a, t dbSNP:370935410
2088 2088 -, cta dbSNP:537108675
2089 2089 c, t dbSNP:75063448
2097 2097 c, t dbSNP:776851181
2107 2107 a, g dbSNP:529429768
2116 2116 g, t dbSNP:549474805
2130 2130 a, g dbSNP:556830972
2203 2203 c, t dbSNP:575186130
2282 2282 c, t dbSNP:545658086
2283 2283 -, tc dbSNP:201313100
2287 2287 c, g dbSNP:144568330
2289 2289 -, t dbSNP:36092308
2290 2290 -, c, t dbSNP:11436510
2291 2291 c, t dbSNP:200704026
2304 2304 -, t dbSNP:397735497
2371 2371 a, c dbSNP:161620
2374 2374 c, t dbSNP:559807250
2381 2381 a, g dbSNP:549833149
2401 2401 c, t dbSNP:548787621
2477 2477 a, c, g dbSNP:113536060
2510 2510 a, g dbSNP:763493186
2531 2531 c, t dbSNP:148453821
2549 2549 a, g dbSNP:572871169
2566 2566 c, t dbSNP:552879065
2588 2588 a, g dbSNP:558231884
2590 2590 c, t dbSNP:374021788
2591 2591 a, g, t dbSNP:111309126
2618 2618 a, g dbSNP:534589177
2622 2622 -, ttttagaa dbSNP:71157674
2627 2627 -, gaatttta dbSNP:777455116
2628 2628 -, aattttag dbSNP:748804734
2629 2629 a, t dbSNP:138968865
2630 2630 -, ttttagaa dbSNP:397717945
2631 2631 -, tttagaag dbSNP:201722515
2682 2682 g, t dbSNP:537388227
2717 2717 a, g, t dbSNP:556943624
2729 2729 c, t dbSNP:765883953
2734 2734 a, g dbSNP:546236128
2741 2741 a, t dbSNP:7210335
2748 2748 c, t dbSNP:568149292
2752 2752 a, c, g dbSNP:377751565
2819 2819 a, c dbSNP:571386177
2872 2872 a, g dbSNP:367885331
2940 2940 c, t dbSNP:575079591
2944 2944 g, t dbSNP:775865016
2988 2988 c, t dbSNP:539395778
3014 3014 a, t dbSNP:370221388
3044 3044 c, t dbSNP:754518597
3047 3047 a, g dbSNP:192778595
3058 3058 c, t dbSNP:573802724
3063 3063 c, t dbSNP:542411157
3140 3140 c, t dbSNP:758226811
3165 3165 a, t dbSNP:9902963
3172 3172 a, t dbSNP:692180
3176 3176 c, t dbSNP:141802218
3184 3184 a, g dbSNP:776939484
3194 3194 g, t dbSNP:544700252
3210 3210 c, t dbSNP:183542930
3241 3241 c, g dbSNP:770763503
3261 3261 a, t dbSNP:146025743
3271 3271 c, t dbSNP:563896823
3388 3388 c, t dbSNP:372226654
3393 3393 c, t dbSNP:552942446
3425 3425 g, t dbSNP:376601294
3495 3495 c, g dbSNP:115371692
3502 3502 a, t dbSNP:374104069
3551 3551 c, t dbSNP:143133684
3552 3552 a, g dbSNP:546598294
3608 3608 c, t dbSNP:562655160
3609 3609 a, g dbSNP:76156285
3666 3666 c, t dbSNP:763118529
3688 3688 c, g, t dbSNP:186556135
3703 3703 a, g dbSNP:774929925
3750 3750 -, t dbSNP:113390675
3800 3800 a, g dbSNP:146698137
3854 3854 g, t dbSNP:191738448
3863 3863 -, a dbSNP:755732323
3876 3876 c, t dbSNP:539257353
3924 3924 c, t dbSNP:139115520
3929 3929 a, g dbSNP:762083428
3992 3992 c, g, t dbSNP:566449657
4009 4009 c, t dbSNP:533168611
4034 4034 c, t dbSNP:149904153
4052 4052 a, t dbSNP:145828729
4053 4053 g, t dbSNP:753514553

Target ORF information:

RefSeq Version NM_001270397
Organism Homo sapiens (human)
Definition Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001270397

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATTA A

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.