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G6PC cDNA ORF clone, Homo sapiens (human)

Gene Symbol G6PC
Entrez Gene ID 2538
Full Name glucose-6-phosphatase, catalytic subunit
Synonyms G6PC1, G6PT, GSD1, GSD1a
General protein information
Preferred Names
glucose-6-phosphatase
Names
glucose-6-phosphatase
G6Pase
G-6-Pase
G6Pase-alpha
glucose-6-phosphatase alpha
NP_000142.1
NP_000142.2
NP_001257326.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21

Summary Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

613742


mRNA and Protein(s)

mRNA Protein Name
NM_000151 NP_000142 glucose-6-phosphatase isoform 1
NM_001270397 NP_001257326 glucose-6-phosphatase isoform 2



Homo sapiens (human) G6PC NP_000142.2
Pan troglodytes (chimpanzee) G6PC XP_003315515.1
Macaca mulatta (Rhesus monkey) G6PC XP_001112333.1
Canis lupus familiaris (dog) G6PC NP_001002993.1
Bos taurus (cattle) G6PC NP_001069592.1
Mus musculus (house mouse) G6pc NP_032087.2
Rattus norvegicus (Norway rat) G6pc NP_037230.2
Gallus gallus (chicken) LOC100857298 XP_003642865.1
Danio rerio (zebrafish) g6pca.2 NP_001157278.1
Xenopus (Silurana) tropicalis (western clawed frog) g6pc2 NP_001096531.1
Xenopus (Silurana) tropicalis (western clawed frog) g6pc NP_001005682.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following G6PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G6PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20803
NM_000151 Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu21715 NM_001270397 Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20803
Clone ID Related Accession (Same CDS sequence) NM_000151
Accession Version NM_000151.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucose-6-phosphatase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, U01120.1, AC016889.28 and AW614228.1. On Jul 3, 2012 this sequence version replaced gi:119393889. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: U01120.1, BC130478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000142.2
CDS81..1154
Misc Feature(1)60..62(+)
Misc Feature(2)165..227(+)
Misc Feature(3)195..656(+)
Misc Feature(4)204..920(+)
Misc Feature(5)261..323(+)
Misc Feature(6)306..620(+)
Misc Feature(7)432..494(+)
Misc Feature(8)522..584(+)
Misc Feature(9)618..686(+)
Misc Feature(10)708..770(+)
Misc Feature(11)843..905(+)
Misc Feature(12)954..1016(+)
Misc Feature(13)1041..1103(+)
Exon (1)1..310
Gene:G6PC
Gene Synonym:
Exon (2)311..420
Gene:G6PC
Gene Synonym:
Exon (3)421..526
Gene:G6PC
Gene Synonym:
Exon (4)527..642
Gene:G6PC
Gene Synonym:
Exon (5)643..4161
Gene:G6PC
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000151
Organism Homo sapiens (human)
Definition Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21715
Clone ID Related Accession (Same CDS sequence) NM_001270397
Accession Version NM_001270397.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucose-6-phosphatase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, BC130478.1, AC016889.28 and AW614228.1. Transcript Variant: This variant (2) lacks an internal segment in the coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK303771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257326.1
CDS81..611
Misc Feature(1)60..62(+)
Misc Feature(2)165..227(+)
Misc Feature(3)204..>422(+)
Misc Feature(4)261..323(+)
Exon (1)1..310
Gene:G6PC
Gene Synonym:
Exon (2)311..420
Gene:G6PC
Gene Synonym:
Exon (3)421..449
Gene:G6PC
Gene Synonym:
Exon (4)450..565
Gene:G6PC
Gene Synonym:
Exon (5)566..4084
Gene:G6PC
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001270397
Organism Homo sapiens (human)
Definition Homo sapiens glucose-6-phosphatase, catalytic subunit (G6PC), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270397

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A novel homozygous no-stop mutation in G6PC gene from a Chinese patient with glycogen storage disease type Ia
Gene 536 (2), 362-365 (2014)
Gu LL, Li XH, Han Y, Zhang DH, Gong QM and Zhang XX.


book

Toll-like receptor 4 signaling is required for induction of gluconeogenic gene expression by palmitate in human hepatic carcinoma cells
J. Nutr. Biochem. 24 (8), 1499-1507 (2013)
Mamedova LK, Yuan K, Laudick AN, Fleming SD, Mashek DG and Bradford BJ.


book

Suppression of gluconeogenic gene expression by LSD1-mediated histone demethylation
PLoS ONE 8 (6), E66294 (2013)
Pan D, Mao C and Wang YX.


book

New insights into the organisation and intracellular localisation of the two subunits of glucose-6-phosphatase
Biochimie 94 (3), 695-703 (2012)
Soty M, Chilloux J, Casteras S, Grichine A, Mithieux G and Gautier-Stein A.


book

DNA damage induces down-regulation of PEPCK and G6P gene expression through degradation of PGC-1alpha
Acta Biochim. Biophys. Sin. (Shanghai) 43 (8), 589-594 (2011)
Kim HJ, Jee HJ and Yun J.


book

Glycogen Storage Disease Type I
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bali,D.S., Chen,Y.T. and Goldstein,J.L.


book

Diagnosis of a novel glycogen storage disease: type 1aSP
J. Inherit. Metab. Dis. 13 (3), 247-249 (1990)
Burchell A and Waddell ID.


book

The microsomal glucose-6-phosphatase enzyme of human gall-bladder
J. Pathol. 158 (1), 53-56 (1989)
Hill A, Waddell ID, Hopwood D and Burchell A.


book

A new variant of glycogen storage disease type I probably due to a defect in the glucose-6-phosphate transport system
Biochem. Biophys. Res. Commun. 83 (4), 1360-1364 (1978)
Narisawa,K., Igarashi,Y., Otomo,H. and Tada,K.


book

The fine structural localization of testicular phosphatases in man: the control testis
Cell Tissue Res. 166 (4), 497-510 (1976)
Barham,S.S., Berlin,J.D. and Brackeen,R.B.


 
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