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GABRA1 gamma-aminobutyric acid (GABA) A receptor, alpha 1 [Homo sapiens (human)]

Gene Symbol GABRA1
Entrez Gene ID 2554
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 1
Synonyms ECA4, EIEE19, EJM, EJM5
General protein information
Preferred Names
gamma-aminobutyric acid receptor subunit alpha-1
Names
gamma-aminobutyric acid receptor subunit alpha-1
GABA(A) receptor, alpha 1
GABA(A) receptor subunit alpha-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q34

Summary This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

137160

Disorder Html: {Epilepsy, juvenile myoclonic, susceptibility to, 5}, 611136 (3);





The following GABRA1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GABRA1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26115 NM_000806 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379
OHu26115 NM_001127643 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379
OHu26115 NM_001127644 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379
OHu26115 NM_001127645 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379
OHu26115 NM_001127648 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu26115
Accession Version NM_000806.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product gamma-aminobutyric acid receptor subunit alpha-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289792.1, X14766.1 and AC021079.4. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced gi:156071483. Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) uses the 5'-most alternate exon in the 5' UTR. Variants 1-7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289792.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000797.2
CDS469..1839
Misc Feature(1)451..453(+)
Misc Feature(2)508..1794(+)
Misc Feature(3)595..1218(+)
Misc Feature(4)1222..1287(+)
Misc Feature(5)1237..1785(+)
Misc Feature(6)1303..1368(+)
Misc Feature(7)1405..1470(+)
Misc Feature(8)1732..1797(+)
Exon (1)1..221
Gene:GABRA1
Gene Synonym:
Exon (2)222..453
Gene:GABRA1
Gene Synonym:
Exon (3)454..542
Gene:GABRA1
Gene Synonym:
Exon (4)543..655
Gene:GABRA1
Gene Synonym:
Exon (5)656..723
Gene:GABRA1
Gene Synonym:
Exon (6)724..944
Gene:GABRA1
Gene Synonym:
Exon (7)945..1027
Gene:GABRA1
Gene Synonym:
Exon (8)1028..1171
Gene:GABRA1
Gene Synonym:
Exon (9)1172..1324
Gene:GABRA1
Gene Synonym:
Exon (10)1325..1527
Gene:GABRA1
Gene Synonym:
Exon (11)1528..4376
Gene:GABRA1
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:531705924
20 20 -, c dbSNP:141813020
20 20 c, g dbSNP:190043578
20 20 -, g dbSNP:201310567
21 21 a, c, g dbSNP:11576000
22 22 -, c dbSNP:199848639
22 22 c, g, t dbSNP:184629690
23 23 c, g dbSNP:28364635
28 28 -, a dbSNP:766011949
28 28 a, g dbSNP:11576001
29 29 a, g dbSNP:549040995
31 31 -, a dbSNP:112361424
64 64 c, t dbSNP:181962869
79 79 c, t dbSNP:537839796
90 90 a, t dbSNP:555931218
91 91 a, g dbSNP:745504923
98 98 a, g dbSNP:185145997
99 99 a, t dbSNP:766055910
121 121 c, g dbSNP:538555519
131 131 a, g dbSNP:368385541
167 167 a, c dbSNP:189181924
168 168 a, g dbSNP:758048771
178 178 a, g dbSNP:553515856
210 210 a, c dbSNP:571929002
249 249 c, t dbSNP:186477194
301 301 c, t dbSNP:531965222
307 307 a, g dbSNP:547029877
327 327 a, g dbSNP:12658835
347 347 a, g dbSNP:536165896
349 349 -, cggact dbSNP:527890421
367 367 a, g dbSNP:548332850
370 370 -, g dbSNP:371702713
371 371 c, g dbSNP:569773254
416 416 a, c dbSNP:75583203
438 438 c, t dbSNP:4608967
446 446 c, g dbSNP:753121702
455 455 g, t dbSNP:778424128
459 459 c, g dbSNP:376699435
465 465 c, t dbSNP:375475234
466 466 a, g dbSNP:574411082
468 468 a, g, t dbSNP:376266596
476 476 a, g dbSNP:111452646
486 486 c, t dbSNP:557682926
495 495 a, c, t dbSNP:113886269
500 500 c, t dbSNP:762939760
501 501 c, t dbSNP:766418049
508 508 c, t dbSNP:774587445
513 513 c, g dbSNP:759784427
514 514 c, t dbSNP:767887700
521 521 c, t dbSNP:779065852
522 522 c, g dbSNP:753085546
527 527 c, t dbSNP:756553428
536 536 c, g dbSNP:199819387
543 543 a, c, t dbSNP:75423500
546 546 c, t dbSNP:779666888
553 553 c, t dbSNP:143815396
554 554 c, t dbSNP:200218956
555 555 a, g dbSNP:374399356
561 561 a, c dbSNP:747927213
562 562 a, c dbSNP:769743354
564 564 a, g dbSNP:76224028
582 582 c, t dbSNP:543190682
620 620 a, g dbSNP:745932197
624 624 c, t dbSNP:1129647
627 627 c, t dbSNP:775714231
639 639 a, g dbSNP:760892094
643 643 a, c dbSNP:768778314
645 645 a, g dbSNP:777010469
651 651 a, g dbSNP:762124345
661 661 a, g dbSNP:11549458
666 666 c, g, t dbSNP:770208588
667 667 a, g dbSNP:763403354
669 669 a, g dbSNP:766903604
675 675 g, t dbSNP:751050781
684 684 c, t dbSNP:200716712
693 693 a, c dbSNP:547903306
699 699 c, t dbSNP:566224462
705 705 c, t dbSNP:536603724
720 720 c, t dbSNP:777649753
733 733 a, g dbSNP:146428720
738 738 c, t dbSNP:757165660
747 747 c, t dbSNP:267600528
756 756 c, t dbSNP:534779853
765 765 c, t dbSNP:750394365
766 766 a, g dbSNP:267600529
771 771 a, g dbSNP:758360317
773 773 g, t dbSNP:781084915
796 796 a, g dbSNP:552868295
803 803 a, g dbSNP:587777308
832 832 c, t dbSNP:1129648
846 846 a, g dbSNP:756299135
853 853 c, t dbSNP:146134200
858 858 c, t dbSNP:778019249
860 860 a, g dbSNP:749642686
876 876 c, t dbSNP:771269673
886 886 a, g dbSNP:199689997
892 892 c, t dbSNP:746306844
897 897 c, t dbSNP:772653297
904 904 c, t dbSNP:775096518
907 907 c, t dbSNP:139163545
909 909 g, t dbSNP:190024862
926 926 c, t dbSNP:776482117
927 927 c, t dbSNP:761693717
968 968 c, t dbSNP:750728250
969 969 a, g dbSNP:200750234
986 986 -, c dbSNP:35223687
989 989 -, c dbSNP:138145692
997 997 a, g dbSNP:780700439
999 999 a, c dbSNP:747452052
1008 1008 c, t dbSNP:769299843
1011 1011 a, c dbSNP:780800247
1014 1014 a, g dbSNP:747735903
1051 1051 a, g dbSNP:761791288
1070 1070 a, g dbSNP:765292293
1071 1071 c, g dbSNP:773177131
1079 1079 a, g dbSNP:777320447
1080 1080 c, t dbSNP:374616425
1101 1101 c, t dbSNP:587780947
1108 1108 a, c dbSNP:727503940
1123 1123 a, g dbSNP:587777364
1127 1127 g, t dbSNP:755451924
1128 1128 c, t dbSNP:767907251
1132 1132 a, g dbSNP:753182955
1134 1134 a, g dbSNP:756594849
1140 1140 a, g dbSNP:372742479
1145 1145 a, g dbSNP:748783999
1167 1167 c, t dbSNP:756876744
1169 1169 c, t dbSNP:778581160
1179 1179 c, t dbSNP:774587794
1193 1193 c, t dbSNP:759786297
1197 1197 c, t dbSNP:772218052
1219 1219 a, g dbSNP:587777307
1224 1224 a, c, t dbSNP:775895535
1241 1241 a, c dbSNP:764645086
1250 1250 a, g dbSNP:754406560
1290 1290 c, t dbSNP:142477850
1299 1299 a, g dbSNP:764666718
1302 1302 c, t dbSNP:749956729
1308 1308 a, g dbSNP:150497014
1314 1314 a, g dbSNP:139467768
1319 1319 c, t dbSNP:794727962
1333 1333 a, t dbSNP:189199636
1341 1341 c, g, t dbSNP:777911034
1354 1354 c, t dbSNP:149677108
1355 1355 a, t dbSNP:532607986
1376 1376 c, t dbSNP:267600530
1385 1385 a, c dbSNP:587777309
1412 1412 g, t dbSNP:747138999
1422 1422 c, t dbSNP:551045474
1428 1428 c, t dbSNP:761921247
1433 1433 a, c dbSNP:121434579
1434 1434 c, t dbSNP:201133616
1440 1440 g, t dbSNP:145617915
1444 1444 c, t dbSNP:527416473
1467 1467 a, t dbSNP:748550764
1476 1476 c, t dbSNP:200228901
1476 1476 -, t dbSNP:778743668
1487 1487 a, g dbSNP:773775590
1494 1494 c, t dbSNP:750925935
1538 1538 a, t dbSNP:749707253
1546 1546 c, t dbSNP:771498937
1547 1547 a, c dbSNP:80337021
1554 1554 c, t dbSNP:187648029
1563 1563 c, t dbSNP:558548302
1572 1572 c, t dbSNP:771832995
1573 1573 a, g, t dbSNP:775344663
1580 1580 c, t dbSNP:372570305
1590 1590 c, t dbSNP:370816784
1595 1595 a, c dbSNP:761617651
1596 1596 c, g dbSNP:765261463
1598 1598 c, t dbSNP:751571034
1605 1605 a, g dbSNP:755109332
1609 1609 a, c dbSNP:371470198
1614 1614 a, c, t dbSNP:79593368
1615 1615 a, g dbSNP:375377575
1619 1619 c, g, t dbSNP:755336024
1620 1620 c, g dbSNP:777957860
1623 1623 a, c dbSNP:41308303
1624 1624 g, t dbSNP:771316858
1647 1647 a, c dbSNP:779528536
1670 1670 a, g dbSNP:745362654
1674 1674 c, t dbSNP:541335259
1675 1675 a, c, g dbSNP:775157869
1679 1679 c, g dbSNP:768389200
1680 1680 a, c dbSNP:776260370
1686 1686 a, g dbSNP:371392768
1687 1687 c, t dbSNP:765072998
1693 1693 a, c, t dbSNP:139793542
1697 1697 a, g dbSNP:376031361
1704 1704 a, c dbSNP:767535362
1712 1712 c, g dbSNP:752803296
1728 1728 c, t dbSNP:760920254
1729 1729 a, c dbSNP:756295089
1730 1730 a, g dbSNP:778103654
1732 1732 c, t dbSNP:754135872
1734 1734 c, g dbSNP:753473056
1737 1737 a, c dbSNP:756726029
1751 1751 c, t dbSNP:778447396
1752 1752 a, g dbSNP:74873701
1790 1790 c, t dbSNP:145217327
1791 1791 a, g dbSNP:138259457
1815 1815 a, g dbSNP:746560774
1818 1818 a, g dbSNP:142385746
1822 1822 c, t dbSNP:776405072
1825 1825 a, g, t dbSNP:747892371
1828 1828 c, t dbSNP:773012684
1841 1841 a, t dbSNP:762836110
1852 1852 c, t dbSNP:766341369
1866 1866 c, g dbSNP:369682969
1878 1878 a, g dbSNP:201409177
1882 1882 a, g dbSNP:760295110
1894 1894 a, t dbSNP:560848217
1900 1900 c, t dbSNP:574105922
1907 1907 a, g dbSNP:770608742
1923 1923 c, t dbSNP:543408244
1943 1943 c, t dbSNP:746867194
1963 1963 a, g dbSNP:190770358
1965 1965 a, g dbSNP:775983590
2024 2024 a, c dbSNP:2168775
2067 2067 a, g dbSNP:547423635
2098 2098 g, t dbSNP:565859469
2121 2121 a, c dbSNP:34235595
2135 2135 -, a dbSNP:547262225
2164 2164 a, t dbSNP:76047934
2237 2237 a, c dbSNP:374845401
2240 2240 a, g dbSNP:541504060
2246 2246 c, t dbSNP:539475780
2272 2272 c, g dbSNP:140183311
2281 2281 a, t dbSNP:41311749
2286 2286 a, t dbSNP:537161509
2309 2309 a, g dbSNP:2290732
2350 2350 a, g dbSNP:372806449
2358 2358 g, t dbSNP:570515140
2406 2406 -, a dbSNP:113406057
2407 2407 -, a dbSNP:748975579
2407 2407 a, g dbSNP:80203789
2419 2419 c, g dbSNP:534982201
2469 2469 c, t dbSNP:578077816
2476 2476 a, g dbSNP:769792774
2478 2478 a, g dbSNP:187108662
2528 2528 g, t dbSNP:576074789
2568 2568 a, t dbSNP:574956160
2573 2573 a, t dbSNP:762913227
2645 2645 c, t dbSNP:542030537
2670 2670 c, t dbSNP:557546790
2684 2684 a, g dbSNP:576166615
2691 2691 c, t dbSNP:190302700
2720 2720 g, t dbSNP:141928377
2721 2721 a, g dbSNP:766942549
2767 2767 a, t dbSNP:564873207
2776 2776 a, c dbSNP:41315924
2785 2785 a, c dbSNP:139554086
2798 2798 g, t dbSNP:559414972
2810 2810 a, c dbSNP:369546620
2827 2827 a, g dbSNP:182930988
2848 2848 a, g dbSNP:142860832
2880 2880 a, t dbSNP:569376392
2883 2883 a, g dbSNP:563152013
2886 2886 a, g dbSNP:753466444
2900 2900 c, t dbSNP:374002978
2915 2915 c, t dbSNP:531272719
2922 2922 a, g dbSNP:752538724
2947 2947 a, g dbSNP:552298521
3018 3018 c, t dbSNP:377728451
3024 3024 c, t dbSNP:543428415
3092 3092 c, t dbSNP:549431469
3148 3148 a, g dbSNP:570502633
3188 3188 a, c dbSNP:534737930
3206 3206 c, t dbSNP:546995144
3220 3220 c, t dbSNP:187522588
3237 3237 c, t dbSNP:555430982
3260 3260 c, t dbSNP:749987275
3270 3270 a, t dbSNP:536156782
3272 3272 a, t dbSNP:557229266
3282 3282 a, g dbSNP:575959204
3287 3287 c, t dbSNP:193210849
3295 3295 a, g dbSNP:528083481
3335 3335 c, t dbSNP:558722133
3338 3338 a, g dbSNP:546239191
3345 3345 a, c dbSNP:998754
3365 3365 a, g dbSNP:540638714
3387 3387 a, g dbSNP:559313551
3481 3481 c, g dbSNP:746881638
3487 3487 c, g dbSNP:574602367
3492 3492 a, t dbSNP:746582124
3536 3536 a, g dbSNP:75337618
3563 3563 c, t dbSNP:563139650
3591 3591 g, t dbSNP:41303356
3596 3596 a, c dbSNP:151076324
3633 3633 c, g dbSNP:77720837
3634 3634 c, t dbSNP:75746434
3660 3660 g, t dbSNP:10059108
3662 3662 -, t dbSNP:770566138
3709 3709 c, t dbSNP:564215168
3735 3735 a, t dbSNP:368099176
3768 3768 c, t dbSNP:2290733
3788 3788 a, t dbSNP:41311747
3794 3794 c, t dbSNP:376130796
3835 3835 c, t dbSNP:770097153
3848 3848 c, g dbSNP:139565984
3858 3858 a, g dbSNP:535774149
3874 3874 a, g dbSNP:73317010
3911 3911 g, t dbSNP:569460050
3925 3925 c, t dbSNP:773306587
3933 3933 g, t dbSNP:540069540
3937 3937 c, t dbSNP:147837301
3964 3964 g, t dbSNP:189237787
3976 3976 c, t dbSNP:534722665
3979 3979 a, c dbSNP:538462336
3998 3998 -, t dbSNP:149385481
4026 4026 a, g dbSNP:552647468
4029 4029 g, t dbSNP:192225130
4053 4053 a, g dbSNP:141464277
4055 4055 g, t dbSNP:556870416
4118 4118 a, g dbSNP:575186628
4123 4123 a, g dbSNP:545561711
4127 4127 -, c dbSNP:35777559
4160 4160 a, c dbSNP:563948843
4239 4239 a, g dbSNP:528531605
4264 4264 a, g dbSNP:540771612
4303 4303 a, g dbSNP:536520286
4316 4316 g, t dbSNP:554920205
4354 4354 c, t dbSNP:561930118
4362 4362 a, c dbSNP:567624810
4370 4370 a, c dbSNP:774807571

Target ORF information:

RefSeq Version NM_000806
Organism Homo sapiens (human)
Definition Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000806

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26115
Accession Version NM_001127643.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product gamma-aminobutyric acid receptor subunit alpha-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB485936.1, DB487114.1, X14766.1, R60404.1, X13584.1, DA228536.1 and AC021079.4. Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC030696.1, AK289792.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121115.1
CDS356..1726
Misc Feature(1)338..340(+)
Misc Feature(2)395..1681(+)
Misc Feature(3)482..1105(+)
Misc Feature(4)1109..1174(+)
Misc Feature(5)1124..1672(+)
Misc Feature(6)1190..1255(+)
Misc Feature(7)1292..1357(+)
Misc Feature(8)1619..1684(+)
Exon (1)1..108
Gene:GABRA1
Gene Synonym:
Exon (2)109..340
Gene:GABRA1
Gene Synonym:
Exon (3)341..429
Gene:GABRA1
Gene Synonym:
Exon (4)430..542
Gene:GABRA1
Gene Synonym:
Exon (5)543..610
Gene:GABRA1
Gene Synonym:
Exon (6)611..831
Gene:GABRA1
Gene Synonym:
Exon (7)832..914
Gene:GABRA1
Gene Synonym:
Exon (8)915..1058
Gene:GABRA1
Gene Synonym:
Exon (9)1059..1211
Gene:GABRA1
Gene Synonym:
Exon (10)1212..1414
Gene:GABRA1
Gene Synonym:
Exon (11)1415..4263
Gene:GABRA1
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:561037022
25 25 -, c dbSNP:35046751
27 27 c, t dbSNP:531309432
33 33 a, c dbSNP:549709563
58 58 c, t dbSNP:570926746
136 136 c, t dbSNP:186477194
188 188 c, t dbSNP:531965222
194 194 a, g dbSNP:547029877
214 214 a, g dbSNP:12658835
234 234 a, g dbSNP:536165896
236 236 -, cggact dbSNP:527890421
254 254 a, g dbSNP:548332850
257 257 -, g dbSNP:371702713
258 258 c, g dbSNP:569773254
303 303 a, c dbSNP:75583203
325 325 c, t dbSNP:4608967
333 333 c, g dbSNP:753121702
342 342 g, t dbSNP:778424128
346 346 c, g dbSNP:376699435
352 352 c, t dbSNP:375475234
353 353 a, g dbSNP:574411082
355 355 a, g, t dbSNP:376266596
363 363 a, g dbSNP:111452646
373 373 c, t dbSNP:557682926
382 382 a, c, t dbSNP:113886269
387 387 c, t dbSNP:762939760
388 388 c, t dbSNP:766418049
395 395 c, t dbSNP:774587445
400 400 c, g dbSNP:759784427
401 401 c, t dbSNP:767887700
408 408 c, t dbSNP:779065852
409 409 c, g dbSNP:753085546
414 414 c, t dbSNP:756553428
423 423 c, g dbSNP:199819387
430 430 a, c, t dbSNP:75423500
433 433 c, t dbSNP:779666888
440 440 c, t dbSNP:143815396
441 441 c, t dbSNP:200218956
442 442 a, g dbSNP:374399356
448 448 a, c dbSNP:747927213
449 449 a, c dbSNP:769743354
451 451 a, g dbSNP:76224028
469 469 c, t dbSNP:543190682
507 507 a, g dbSNP:745932197
511 511 c, t dbSNP:1129647
514 514 c, t dbSNP:775714231
526 526 a, g dbSNP:760892094
530 530 a, c dbSNP:768778314
532 532 a, g dbSNP:777010469
538 538 a, g dbSNP:762124345
548 548 a, g dbSNP:11549458
553 553 c, g, t dbSNP:770208588
554 554 a, g dbSNP:763403354
556 556 a, g dbSNP:766903604
562 562 g, t dbSNP:751050781
571 571 c, t dbSNP:200716712
580 580 a, c dbSNP:547903306
586 586 c, t dbSNP:566224462
592 592 c, t dbSNP:536603724
607 607 c, t dbSNP:777649753
620 620 a, g dbSNP:146428720
625 625 c, t dbSNP:757165660
634 634 c, t dbSNP:267600528
643 643 c, t dbSNP:534779853
652 652 c, t dbSNP:750394365
653 653 a, g dbSNP:267600529
658 658 a, g dbSNP:758360317
660 660 g, t dbSNP:781084915
683 683 a, g dbSNP:552868295
690 690 a, g dbSNP:587777308
719 719 c, t dbSNP:1129648
733 733 a, g dbSNP:756299135
740 740 c, t dbSNP:146134200
745 745 c, t dbSNP:778019249
747 747 a, g dbSNP:749642686
763 763 c, t dbSNP:771269673
773 773 a, g dbSNP:199689997
779 779 c, t dbSNP:746306844
784 784 c, t dbSNP:772653297
791 791 c, t dbSNP:775096518
794 794 c, t dbSNP:139163545
796 796 g, t dbSNP:190024862
813 813 c, t dbSNP:776482117
814 814 c, t dbSNP:761693717
855 855 c, t dbSNP:750728250
856 856 a, g dbSNP:200750234
873 873 -, c dbSNP:35223687
876 876 -, c dbSNP:138145692
884 884 a, g dbSNP:780700439
886 886 a, c dbSNP:747452052
895 895 c, t dbSNP:769299843
898 898 a, c dbSNP:780800247
901 901 a, g dbSNP:747735903
938 938 a, g dbSNP:761791288
957 957 a, g dbSNP:765292293
958 958 c, g dbSNP:773177131
966 966 a, g dbSNP:777320447
967 967 c, t dbSNP:374616425
988 988 c, t dbSNP:587780947
995 995 a, c dbSNP:727503940
1010 1010 a, g dbSNP:587777364
1014 1014 g, t dbSNP:755451924
1015 1015 c, t dbSNP:767907251
1019 1019 a, g dbSNP:753182955
1021 1021 a, g dbSNP:756594849
1027 1027 a, g dbSNP:372742479
1032 1032 a, g dbSNP:748783999
1054 1054 c, t dbSNP:756876744
1056 1056 c, t dbSNP:778581160
1066 1066 c, t dbSNP:774587794
1080 1080 c, t dbSNP:759786297
1084 1084 c, t dbSNP:772218052
1106 1106 a, g dbSNP:587777307
1111 1111 a, c, t dbSNP:775895535
1128 1128 a, c dbSNP:764645086
1137 1137 a, g dbSNP:754406560
1177 1177 c, t dbSNP:142477850
1186 1186 a, g dbSNP:764666718
1189 1189 c, t dbSNP:749956729
1195 1195 a, g dbSNP:150497014
1201 1201 a, g dbSNP:139467768
1206 1206 c, t dbSNP:794727962
1220 1220 a, t dbSNP:189199636
1228 1228 c, g, t dbSNP:777911034
1241 1241 c, t dbSNP:149677108
1242 1242 a, t dbSNP:532607986
1263 1263 c, t dbSNP:267600530
1272 1272 a, c dbSNP:587777309
1299 1299 g, t dbSNP:747138999
1309 1309 c, t dbSNP:551045474
1315 1315 c, t dbSNP:761921247
1320 1320 a, c dbSNP:121434579
1321 1321 c, t dbSNP:201133616
1327 1327 g, t dbSNP:145617915
1331 1331 c, t dbSNP:527416473
1354 1354 a, t dbSNP:748550764
1363 1363 c, t dbSNP:200228901
1363 1363 -, t dbSNP:778743668
1374 1374 a, g dbSNP:773775590
1381 1381 c, t dbSNP:750925935
1425 1425 a, t dbSNP:749707253
1433 1433 c, t dbSNP:771498937
1434 1434 a, c dbSNP:80337021
1441 1441 c, t dbSNP:187648029
1450 1450 c, t dbSNP:558548302
1459 1459 c, t dbSNP:771832995
1460 1460 a, g, t dbSNP:775344663
1467 1467 c, t dbSNP:372570305
1477 1477 c, t dbSNP:370816784
1482 1482 a, c dbSNP:761617651
1483 1483 c, g dbSNP:765261463
1485 1485 c, t dbSNP:751571034
1492 1492 a, g dbSNP:755109332
1496 1496 a, c dbSNP:371470198
1501 1501 a, c, t dbSNP:79593368
1502 1502 a, g dbSNP:375377575
1506 1506 c, g, t dbSNP:755336024
1507 1507 c, g dbSNP:777957860
1510 1510 a, c dbSNP:41308303
1511 1511 g, t dbSNP:771316858
1534 1534 a, c dbSNP:779528536
1557 1557 a, g dbSNP:745362654
1561 1561 c, t dbSNP:541335259
1562 1562 a, c, g dbSNP:775157869
1566 1566 c, g dbSNP:768389200
1567 1567 a, c dbSNP:776260370
1573 1573 a, g dbSNP:371392768
1574 1574 c, t dbSNP:765072998
1580 1580 a, c, t dbSNP:139793542
1584 1584 a, g dbSNP:376031361
1591 1591 a, c dbSNP:767535362
1599 1599 c, g dbSNP:752803296
1615 1615 c, t dbSNP:760920254
1616 1616 a, c dbSNP:756295089
1617 1617 a, g dbSNP:778103654
1619 1619 c, t dbSNP:754135872
1621 1621 c, g dbSNP:753473056
1624 1624 a, c dbSNP:756726029
1638 1638 c, t dbSNP:778447396
1639 1639 a, g dbSNP:74873701
1677 1677 c, t dbSNP:145217327
1678 1678 a, g dbSNP:138259457
1702 1702 a, g dbSNP:746560774
1705 1705 a, g dbSNP:142385746
1709 1709 c, t dbSNP:776405072
1712 1712 a, g, t dbSNP:747892371
1715 1715 c, t dbSNP:773012684
1728 1728 a, t dbSNP:762836110
1739 1739 c, t dbSNP:766341369
1753 1753 c, g dbSNP:369682969
1765 1765 a, g dbSNP:201409177
1769 1769 a, g dbSNP:760295110
1781 1781 a, t dbSNP:560848217
1787 1787 c, t dbSNP:574105922
1794 1794 a, g dbSNP:770608742
1810 1810 c, t dbSNP:543408244
1830 1830 c, t dbSNP:746867194
1850 1850 a, g dbSNP:190770358
1852 1852 a, g dbSNP:775983590
1911 1911 a, c dbSNP:2168775
1954 1954 a, g dbSNP:547423635
1985 1985 g, t dbSNP:565859469
2008 2008 a, c dbSNP:34235595
2022 2022 -, a dbSNP:547262225
2051 2051 a, t dbSNP:76047934
2124 2124 a, c dbSNP:374845401
2127 2127 a, g dbSNP:541504060
2133 2133 c, t dbSNP:539475780
2159 2159 c, g dbSNP:140183311
2168 2168 a, t dbSNP:41311749
2173 2173 a, t dbSNP:537161509
2196 2196 a, g dbSNP:2290732
2237 2237 a, g dbSNP:372806449
2245 2245 g, t dbSNP:570515140
2293 2293 -, a dbSNP:113406057
2294 2294 -, a dbSNP:748975579
2294 2294 a, g dbSNP:80203789
2306 2306 c, g dbSNP:534982201
2356 2356 c, t dbSNP:578077816
2363 2363 a, g dbSNP:769792774
2365 2365 a, g dbSNP:187108662
2415 2415 g, t dbSNP:576074789
2455 2455 a, t dbSNP:574956160
2460 2460 a, t dbSNP:762913227
2532 2532 c, t dbSNP:542030537
2557 2557 c, t dbSNP:557546790
2571 2571 a, g dbSNP:576166615
2578 2578 c, t dbSNP:190302700
2607 2607 g, t dbSNP:141928377
2608 2608 a, g dbSNP:766942549
2654 2654 a, t dbSNP:564873207
2663 2663 a, c dbSNP:41315924
2672 2672 a, c dbSNP:139554086
2685 2685 g, t dbSNP:559414972
2697 2697 a, c dbSNP:369546620
2714 2714 a, g dbSNP:182930988
2735 2735 a, g dbSNP:142860832
2767 2767 a, t dbSNP:569376392
2770 2770 a, g dbSNP:563152013
2773 2773 a, g dbSNP:753466444
2787 2787 c, t dbSNP:374002978
2802 2802 c, t dbSNP:531272719
2809 2809 a, g dbSNP:752538724
2834 2834 a, g dbSNP:552298521
2905 2905 c, t dbSNP:377728451
2911 2911 c, t dbSNP:543428415
2979 2979 c, t dbSNP:549431469
3035 3035 a, g dbSNP:570502633
3075 3075 a, c dbSNP:534737930
3093 3093 c, t dbSNP:546995144
3107 3107 c, t dbSNP:187522588
3124 3124 c, t dbSNP:555430982
3147 3147 c, t dbSNP:749987275
3157 3157 a, t dbSNP:536156782
3159 3159 a, t dbSNP:557229266
3169 3169 a, g dbSNP:575959204
3174 3174 c, t dbSNP:193210849
3182 3182 a, g dbSNP:528083481
3222 3222 c, t dbSNP:558722133
3225 3225 a, g dbSNP:546239191
3232 3232 a, c dbSNP:998754
3252 3252 a, g dbSNP:540638714
3274 3274 a, g dbSNP:559313551
3368 3368 c, g dbSNP:746881638
3374 3374 c, g dbSNP:574602367
3379 3379 a, t dbSNP:746582124
3423 3423 a, g dbSNP:75337618
3450 3450 c, t dbSNP:563139650
3478 3478 g, t dbSNP:41303356
3483 3483 a, c dbSNP:151076324
3520 3520 c, g dbSNP:77720837
3521 3521 c, t dbSNP:75746434
3547 3547 g, t dbSNP:10059108
3549 3549 -, t dbSNP:770566138
3596 3596 c, t dbSNP:564215168
3622 3622 a, t dbSNP:368099176
3655 3655 c, t dbSNP:2290733
3675 3675 a, t dbSNP:41311747
3681 3681 c, t dbSNP:376130796
3722 3722 c, t dbSNP:770097153
3735 3735 c, g dbSNP:139565984
3745 3745 a, g dbSNP:535774149
3761 3761 a, g dbSNP:73317010
3798 3798 g, t dbSNP:569460050
3812 3812 c, t dbSNP:773306587
3820 3820 g, t dbSNP:540069540
3824 3824 c, t dbSNP:147837301
3851 3851 g, t dbSNP:189237787
3863 3863 c, t dbSNP:534722665
3866 3866 a, c dbSNP:538462336
3885 3885 -, t dbSNP:149385481
3913 3913 a, g dbSNP:552647468
3916 3916 g, t dbSNP:192225130
3940 3940 a, g dbSNP:141464277
3942 3942 g, t dbSNP:556870416
4005 4005 a, g dbSNP:575186628
4010 4010 a, g dbSNP:545561711
4014 4014 -, c dbSNP:35777559
4047 4047 a, c dbSNP:563948843
4126 4126 a, g dbSNP:528531605
4151 4151 a, g dbSNP:540771612
4190 4190 a, g dbSNP:536520286
4203 4203 g, t dbSNP:554920205
4241 4241 c, t dbSNP:561930118
4249 4249 a, c dbSNP:567624810
4257 4257 a, c dbSNP:774807571

Target ORF information:

RefSeq Version NM_001127643
Organism Homo sapiens (human)
Definition Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127643

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26115
Accession Version NM_001127644.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product gamma-aminobutyric acid receptor subunit alpha-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA075784.1, X14766.1, R60404.1, DA228536.1 and AC021079.4. Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X14766.1, AK223447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121116.1
CDS505..1875
Misc Feature(1)487..489(+)
Misc Feature(2)544..1830(+)
Misc Feature(3)631..1254(+)
Misc Feature(4)1258..1323(+)
Misc Feature(5)1273..1821(+)
Misc Feature(6)1339..1404(+)
Misc Feature(7)1441..1506(+)
Misc Feature(8)1768..1833(+)
Exon (1)1..489
Gene:GABRA1
Gene Synonym:
Exon (2)490..578
Gene:GABRA1
Gene Synonym:
Exon (3)579..691
Gene:GABRA1
Gene Synonym:
Exon (4)692..759
Gene:GABRA1
Gene Synonym:
Exon (5)760..980
Gene:GABRA1
Gene Synonym:
Exon (6)981..1063
Gene:GABRA1
Gene Synonym:
Exon (7)1064..1207
Gene:GABRA1
Gene Synonym:
Exon (8)1208..1360
Gene:GABRA1
Gene Synonym:
Exon (9)1361..1563
Gene:GABRA1
Gene Synonym:
Exon (10)1564..4412
Gene:GABRA1
Gene Synonym:
Translation
Position Chain Variation Link
23 23 c, t dbSNP:190763101
29 29 a, t dbSNP:573553325
37 37 c, g dbSNP:542361381
112 112 g, t dbSNP:563737368
150 150 g, t dbSNP:531399180
242 242 a, g dbSNP:763127240
247 247 c, t dbSNP:183280626
248 248 c, t dbSNP:587780946
253 253 c, t dbSNP:766490931
285 285 c, t dbSNP:186477194
337 337 c, t dbSNP:531965222
343 343 a, g dbSNP:547029877
363 363 a, g dbSNP:12658835
383 383 a, g dbSNP:536165896
385 385 -, cggact dbSNP:527890421
403 403 a, g dbSNP:548332850
406 406 -, g dbSNP:371702713
407 407 c, g dbSNP:569773254
452 452 a, c dbSNP:75583203
474 474 c, t dbSNP:4608967
482 482 c, g dbSNP:753121702
491 491 g, t dbSNP:778424128
495 495 c, g dbSNP:376699435
501 501 c, t dbSNP:375475234
502 502 a, g dbSNP:574411082
504 504 a, g, t dbSNP:376266596
512 512 a, g dbSNP:111452646
522 522 c, t dbSNP:557682926
531 531 a, c, t dbSNP:113886269
536 536 c, t dbSNP:762939760
537 537 c, t dbSNP:766418049
544 544 c, t dbSNP:774587445
549 549 c, g dbSNP:759784427
550 550 c, t dbSNP:767887700
557 557 c, t dbSNP:779065852
558 558 c, g dbSNP:753085546
563 563 c, t dbSNP:756553428
572 572 c, g dbSNP:199819387
579 579 a, c, t dbSNP:75423500
582 582 c, t dbSNP:779666888
589 589 c, t dbSNP:143815396
590 590 c, t dbSNP:200218956
591 591 a, g dbSNP:374399356
597 597 a, c dbSNP:747927213
598 598 a, c dbSNP:769743354
600 600 a, g dbSNP:76224028
618 618 c, t dbSNP:543190682
656 656 a, g dbSNP:745932197
660 660 c, t dbSNP:1129647
663 663 c, t dbSNP:775714231
675 675 a, g dbSNP:760892094
679 679 a, c dbSNP:768778314
681 681 a, g dbSNP:777010469
687 687 a, g dbSNP:762124345
697 697 a, g dbSNP:11549458
702 702 c, g, t dbSNP:770208588
703 703 a, g dbSNP:763403354
705 705 a, g dbSNP:766903604
711 711 g, t dbSNP:751050781
720 720 c, t dbSNP:200716712
729 729 a, c dbSNP:547903306
735 735 c, t dbSNP:566224462
741 741 c, t dbSNP:536603724
756 756 c, t dbSNP:777649753
769 769 a, g dbSNP:146428720
774 774 c, t dbSNP:757165660
783 783 c, t dbSNP:267600528
792 792 c, t dbSNP:534779853
801 801 c, t dbSNP:750394365
802 802 a, g dbSNP:267600529
807 807 a, g dbSNP:758360317
809 809 g, t dbSNP:781084915
832 832 a, g dbSNP:552868295
839 839 a, g dbSNP:587777308
868 868 c, t dbSNP:1129648
882 882 a, g dbSNP:756299135
889 889 c, t dbSNP:146134200
894 894 c, t dbSNP:778019249
896 896 a, g dbSNP:749642686
912 912 c, t dbSNP:771269673
922 922 a, g dbSNP:199689997
928 928 c, t dbSNP:746306844
933 933 c, t dbSNP:772653297
940 940 c, t dbSNP:775096518
943 943 c, t dbSNP:139163545
945 945 g, t dbSNP:190024862
962 962 c, t dbSNP:776482117
963 963 c, t dbSNP:761693717
1004 1004 c, t dbSNP:750728250
1005 1005 a, g dbSNP:200750234
1022 1022 -, c dbSNP:35223687
1025 1025 -, c dbSNP:138145692
1033 1033 a, g dbSNP:780700439
1035 1035 a, c dbSNP:747452052
1044 1044 c, t dbSNP:769299843
1047 1047 a, c dbSNP:780800247
1050 1050 a, g dbSNP:747735903
1087 1087 a, g dbSNP:761791288
1106 1106 a, g dbSNP:765292293
1107 1107 c, g dbSNP:773177131
1115 1115 a, g dbSNP:777320447
1116 1116 c, t dbSNP:374616425
1137 1137 c, t dbSNP:587780947
1144 1144 a, c dbSNP:727503940
1159 1159 a, g dbSNP:587777364
1163 1163 g, t dbSNP:755451924
1164 1164 c, t dbSNP:767907251
1168 1168 a, g dbSNP:753182955
1170 1170 a, g dbSNP:756594849
1176 1176 a, g dbSNP:372742479
1181 1181 a, g dbSNP:748783999
1203 1203 c, t dbSNP:756876744
1205 1205 c, t dbSNP:778581160
1215 1215 c, t dbSNP:774587794
1229 1229 c, t dbSNP:759786297
1233 1233 c, t dbSNP:772218052
1255 1255 a, g dbSNP:587777307
1260 1260 a, c, t dbSNP:775895535
1277 1277 a, c dbSNP:764645086
1286 1286 a, g dbSNP:754406560
1326 1326 c, t dbSNP:142477850
1335 1335 a, g dbSNP:764666718
1338 1338 c, t dbSNP:749956729
1344 1344 a, g dbSNP:150497014
1350 1350 a, g dbSNP:139467768
1355 1355 c, t dbSNP:794727962
1369 1369 a, t dbSNP:189199636
1377 1377 c, g, t dbSNP:777911034
1390 1390 c, t dbSNP:149677108
1391 1391 a, t dbSNP:532607986
1412 1412 c, t dbSNP:267600530
1421 1421 a, c dbSNP:587777309
1448 1448 g, t dbSNP:747138999
1458 1458 c, t dbSNP:551045474
1464 1464 c, t dbSNP:761921247
1469 1469 a, c dbSNP:121434579
1470 1470 c, t dbSNP:201133616
1476 1476 g, t dbSNP:145617915
1480 1480 c, t dbSNP:527416473
1503 1503 a, t dbSNP:748550764
1512 1512 c, t dbSNP:200228901
1512 1512 -, t dbSNP:778743668
1523 1523 a, g dbSNP:773775590
1530 1530 c, t dbSNP:750925935
1574 1574 a, t dbSNP:749707253
1582 1582 c, t dbSNP:771498937
1583 1583 a, c dbSNP:80337021
1590 1590 c, t dbSNP:187648029
1599 1599 c, t dbSNP:558548302
1608 1608 c, t dbSNP:771832995
1609 1609 a, g, t dbSNP:775344663
1616 1616 c, t dbSNP:372570305
1626 1626 c, t dbSNP:370816784
1631 1631 a, c dbSNP:761617651
1632 1632 c, g dbSNP:765261463
1634 1634 c, t dbSNP:751571034
1641 1641 a, g dbSNP:755109332
1645 1645 a, c dbSNP:371470198
1650 1650 a, c, t dbSNP:79593368
1651 1651 a, g dbSNP:375377575
1655 1655 c, g, t dbSNP:755336024
1656 1656 c, g dbSNP:777957860
1659 1659 a, c dbSNP:41308303
1660 1660 g, t dbSNP:771316858
1683 1683 a, c dbSNP:779528536
1706 1706 a, g dbSNP:745362654
1710 1710 c, t dbSNP:541335259
1711 1711 a, c, g dbSNP:775157869
1715 1715 c, g dbSNP:768389200
1716 1716 a, c dbSNP:776260370
1722 1722 a, g dbSNP:371392768
1723 1723 c, t dbSNP:765072998
1729 1729 a, c, t dbSNP:139793542
1733 1733 a, g dbSNP:376031361
1740 1740 a, c dbSNP:767535362
1748 1748 c, g dbSNP:752803296
1764 1764 c, t dbSNP:760920254
1765 1765 a, c dbSNP:756295089
1766 1766 a, g dbSNP:778103654
1768 1768 c, t dbSNP:754135872
1770 1770 c, g dbSNP:753473056
1773 1773 a, c dbSNP:756726029
1787 1787 c, t dbSNP:778447396
1788 1788 a, g dbSNP:74873701
1826 1826 c, t dbSNP:145217327
1827 1827 a, g dbSNP:138259457
1851 1851 a, g dbSNP:746560774
1854 1854 a, g dbSNP:142385746
1858 1858 c, t dbSNP:776405072
1861 1861 a, g, t dbSNP:747892371
1864 1864 c, t dbSNP:773012684
1877 1877 a, t dbSNP:762836110
1888 1888 c, t dbSNP:766341369
1902 1902 c, g dbSNP:369682969
1914 1914 a, g dbSNP:201409177
1918 1918 a, g dbSNP:760295110
1930 1930 a, t dbSNP:560848217
1936 1936 c, t dbSNP:574105922
1943 1943 a, g dbSNP:770608742
1959 1959 c, t dbSNP:543408244
1979 1979 c, t dbSNP:746867194
1999 1999 a, g dbSNP:190770358
2001 2001 a, g dbSNP:775983590
2060 2060 a, c dbSNP:2168775
2103 2103 a, g dbSNP:547423635
2134 2134 g, t dbSNP:565859469
2157 2157 a, c dbSNP:34235595
2171 2171 -, a dbSNP:547262225
2200 2200 a, t dbSNP:76047934
2273 2273 a, c dbSNP:374845401
2276 2276 a, g dbSNP:541504060
2282 2282 c, t dbSNP:539475780
2308 2308 c, g dbSNP:140183311
2317 2317 a, t dbSNP:41311749
2322 2322 a, t dbSNP:537161509
2345 2345 a, g dbSNP:2290732
2386 2386 a, g dbSNP:372806449
2394 2394 g, t dbSNP:570515140
2442 2442 -, a dbSNP:113406057
2443 2443 -, a dbSNP:748975579
2443 2443 a, g dbSNP:80203789
2455 2455 c, g dbSNP:534982201
2505 2505 c, t dbSNP:578077816
2512 2512 a, g dbSNP:769792774
2514 2514 a, g dbSNP:187108662
2564 2564 g, t dbSNP:576074789
2604 2604 a, t dbSNP:574956160
2609 2609 a, t dbSNP:762913227
2681 2681 c, t dbSNP:542030537
2706 2706 c, t dbSNP:557546790
2720 2720 a, g dbSNP:576166615
2727 2727 c, t dbSNP:190302700
2756 2756 g, t dbSNP:141928377
2757 2757 a, g dbSNP:766942549
2803 2803 a, t dbSNP:564873207
2812 2812 a, c dbSNP:41315924
2821 2821 a, c dbSNP:139554086
2834 2834 g, t dbSNP:559414972
2846 2846 a, c dbSNP:369546620
2863 2863 a, g dbSNP:182930988
2884 2884 a, g dbSNP:142860832
2916 2916 a, t dbSNP:569376392
2919 2919 a, g dbSNP:563152013
2922 2922 a, g dbSNP:753466444
2936 2936 c, t dbSNP:374002978
2951 2951 c, t dbSNP:531272719
2958 2958 a, g dbSNP:752538724
2983 2983 a, g dbSNP:552298521
3054 3054 c, t dbSNP:377728451
3060 3060 c, t dbSNP:543428415
3128 3128 c, t dbSNP:549431469
3184 3184 a, g dbSNP:570502633
3224 3224 a, c dbSNP:534737930
3242 3242 c, t dbSNP:546995144
3256 3256 c, t dbSNP:187522588
3273 3273 c, t dbSNP:555430982
3296 3296 c, t dbSNP:749987275
3306 3306 a, t dbSNP:536156782
3308 3308 a, t dbSNP:557229266
3318 3318 a, g dbSNP:575959204
3323 3323 c, t dbSNP:193210849
3331 3331 a, g dbSNP:528083481
3371 3371 c, t dbSNP:558722133
3374 3374 a, g dbSNP:546239191
3381 3381 a, c dbSNP:998754
3401 3401 a, g dbSNP:540638714
3423 3423 a, g dbSNP:559313551
3517 3517 c, g dbSNP:746881638
3523 3523 c, g dbSNP:574602367
3528 3528 a, t dbSNP:746582124
3572 3572 a, g dbSNP:75337618
3599 3599 c, t dbSNP:563139650
3627 3627 g, t dbSNP:41303356
3632 3632 a, c dbSNP:151076324
3669 3669 c, g dbSNP:77720837
3670 3670 c, t dbSNP:75746434
3696 3696 g, t dbSNP:10059108
3698 3698 -, t dbSNP:770566138
3745 3745 c, t dbSNP:564215168
3771 3771 a, t dbSNP:368099176
3804 3804 c, t dbSNP:2290733
3824 3824 a, t dbSNP:41311747
3830 3830 c, t dbSNP:376130796
3871 3871 c, t dbSNP:770097153
3884 3884 c, g dbSNP:139565984
3894 3894 a, g dbSNP:535774149
3910 3910 a, g dbSNP:73317010
3947 3947 g, t dbSNP:569460050
3961 3961 c, t dbSNP:773306587
3969 3969 g, t dbSNP:540069540
3973 3973 c, t dbSNP:147837301
4000 4000 g, t dbSNP:189237787
4012 4012 c, t dbSNP:534722665
4015 4015 a, c dbSNP:538462336
4034 4034 -, t dbSNP:149385481
4062 4062 a, g dbSNP:552647468
4065 4065 g, t dbSNP:192225130
4089 4089 a, g dbSNP:141464277
4091 4091 g, t dbSNP:556870416
4154 4154 a, g dbSNP:575186628
4159 4159 a, g dbSNP:545561711
4163 4163 -, c dbSNP:35777559
4196 4196 a, c dbSNP:563948843
4275 4275 a, g dbSNP:528531605
4300 4300 a, g dbSNP:540771612
4339 4339 a, g dbSNP:536520286
4352 4352 g, t dbSNP:554920205
4390 4390 c, t dbSNP:561930118
4398 4398 a, c dbSNP:567624810
4406 4406 a, c dbSNP:774807571

Target ORF information:

RefSeq Version NM_001127644
Organism Homo sapiens (human)
Definition Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127644

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26115
Accession Version NM_001127645.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product gamma-aminobutyric acid receptor subunit alpha-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC307259.1, BC030696.1 and AC021079.4. Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1-7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121117.1
CDS93..1463
Misc Feature(1)132..1418(+)
Misc Feature(2)219..842(+)
Misc Feature(3)846..911(+)
Misc Feature(4)861..1409(+)
Misc Feature(5)927..992(+)
Misc Feature(6)1029..1094(+)
Misc Feature(7)1356..1421(+)
Exon (1)1..77
Gene:GABRA1
Gene Synonym:
Exon (2)78..166
Gene:GABRA1
Gene Synonym:
Exon (3)167..279
Gene:GABRA1
Gene Synonym:
Exon (4)280..347
Gene:GABRA1
Gene Synonym:
Exon (5)348..568
Gene:GABRA1
Gene Synonym:
Exon (6)569..651
Gene:GABRA1
Gene Synonym:
Exon (7)652..795
Gene:GABRA1
Gene Synonym:
Exon (8)796..948
Gene:GABRA1
Gene Synonym:
Exon (9)949..1151
Gene:GABRA1
Gene Synonym:
Exon (10)1152..4000
Gene:GABRA1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 -, ga dbSNP:763900656
1 1 -, g dbSNP:34175342
3 3 -, g dbSNP:34330422
10 10 -, ag dbSNP:147082642
15 15 -, ga dbSNP:33991515
23 23 -, gagc dbSNP:200619744
24 24 c, g dbSNP:557499345
27 27 a, c dbSNP:575727290
50 50 a, g dbSNP:745962526
79 79 g, t dbSNP:778424128
83 83 c, g dbSNP:376699435
89 89 c, t dbSNP:375475234
90 90 a, g dbSNP:574411082
92 92 a, g, t dbSNP:376266596
100 100 a, g dbSNP:111452646
110 110 c, t dbSNP:557682926
119 119 a, c, t dbSNP:113886269
124 124 c, t dbSNP:762939760
125 125 c, t dbSNP:766418049
132 132 c, t dbSNP:774587445
137 137 c, g dbSNP:759784427
138 138 c, t dbSNP:767887700
145 145 c, t dbSNP:779065852
146 146 c, g dbSNP:753085546
151 151 c, t dbSNP:756553428
160 160 c, g dbSNP:199819387
167 167 a, c, t dbSNP:75423500
170 170 c, t dbSNP:779666888
177 177 c, t dbSNP:143815396
178 178 c, t dbSNP:200218956
179 179 a, g dbSNP:374399356
185 185 a, c dbSNP:747927213
186 186 a, c dbSNP:769743354
188 188 a, g dbSNP:76224028
206 206 c, t dbSNP:543190682
244 244 a, g dbSNP:745932197
248 248 c, t dbSNP:1129647
251 251 c, t dbSNP:775714231
263 263 a, g dbSNP:760892094
267 267 a, c dbSNP:768778314
269 269 a, g dbSNP:777010469
275 275 a, g dbSNP:762124345
285 285 a, g dbSNP:11549458
290 290 c, g, t dbSNP:770208588
291 291 a, g dbSNP:763403354
293 293 a, g dbSNP:766903604
299 299 g, t dbSNP:751050781
308 308 c, t dbSNP:200716712
317 317 a, c dbSNP:547903306
323 323 c, t dbSNP:566224462
329 329 c, t dbSNP:536603724
344 344 c, t dbSNP:777649753
357 357 a, g dbSNP:146428720
362 362 c, t dbSNP:757165660
371 371 c, t dbSNP:267600528
380 380 c, t dbSNP:534779853
389 389 c, t dbSNP:750394365
390 390 a, g dbSNP:267600529
395 395 a, g dbSNP:758360317
397 397 g, t dbSNP:781084915
420 420 a, g dbSNP:552868295
427 427 a, g dbSNP:587777308
456 456 c, t dbSNP:1129648
470 470 a, g dbSNP:756299135
477 477 c, t dbSNP:146134200
482 482 c, t dbSNP:778019249
484 484 a, g dbSNP:749642686
500 500 c, t dbSNP:771269673
510 510 a, g dbSNP:199689997
516 516 c, t dbSNP:746306844
521 521 c, t dbSNP:772653297
528 528 c, t dbSNP:775096518
531 531 c, t dbSNP:139163545
533 533 g, t dbSNP:190024862
550 550 c, t dbSNP:776482117
551 551 c, t dbSNP:761693717
592 592 c, t dbSNP:750728250
593 593 a, g dbSNP:200750234
610 610 -, c dbSNP:35223687
613 613 -, c dbSNP:138145692
621 621 a, g dbSNP:780700439
623 623 a, c dbSNP:747452052
632 632 c, t dbSNP:769299843
635 635 a, c dbSNP:780800247
638 638 a, g dbSNP:747735903
675 675 a, g dbSNP:761791288
694 694 a, g dbSNP:765292293
695 695 c, g dbSNP:773177131
703 703 a, g dbSNP:777320447
704 704 c, t dbSNP:374616425
725 725 c, t dbSNP:587780947
732 732 a, c dbSNP:727503940
747 747 a, g dbSNP:587777364
751 751 g, t dbSNP:755451924
752 752 c, t dbSNP:767907251
756 756 a, g dbSNP:753182955
758 758 a, g dbSNP:756594849
764 764 a, g dbSNP:372742479
769 769 a, g dbSNP:748783999
791 791 c, t dbSNP:756876744
793 793 c, t dbSNP:778581160
803 803 c, t dbSNP:774587794
817 817 c, t dbSNP:759786297
821 821 c, t dbSNP:772218052
843 843 a, g dbSNP:587777307
848 848 a, c, t dbSNP:775895535
865 865 a, c dbSNP:764645086
874 874 a, g dbSNP:754406560
914 914 c, t dbSNP:142477850
923 923 a, g dbSNP:764666718
926 926 c, t dbSNP:749956729
932 932 a, g dbSNP:150497014
938 938 a, g dbSNP:139467768
943 943 c, t dbSNP:794727962
957 957 a, t dbSNP:189199636
965 965 c, g, t dbSNP:777911034
978 978 c, t dbSNP:149677108
979 979 a, t dbSNP:532607986
1000 1000 c, t dbSNP:267600530
1009 1009 a, c dbSNP:587777309
1036 1036 g, t dbSNP:747138999
1046 1046 c, t dbSNP:551045474
1052 1052 c, t dbSNP:761921247
1057 1057 a, c dbSNP:121434579
1058 1058 c, t dbSNP:201133616
1064 1064 g, t dbSNP:145617915
1068 1068 c, t dbSNP:527416473
1091 1091 a, t dbSNP:748550764
1100 1100 c, t dbSNP:200228901
1100 1100 -, t dbSNP:778743668
1111 1111 a, g dbSNP:773775590
1118 1118 c, t dbSNP:750925935
1162 1162 a, t dbSNP:749707253
1170 1170 c, t dbSNP:771498937
1171 1171 a, c dbSNP:80337021
1178 1178 c, t dbSNP:187648029
1187 1187 c, t dbSNP:558548302
1196 1196 c, t dbSNP:771832995
1197 1197 a, g, t dbSNP:775344663
1204 1204 c, t dbSNP:372570305
1214 1214 c, t dbSNP:370816784
1219 1219 a, c dbSNP:761617651
1220 1220 c, g dbSNP:765261463
1222 1222 c, t dbSNP:751571034
1229 1229 a, g dbSNP:755109332
1233 1233 a, c dbSNP:371470198
1238 1238 a, c, t dbSNP:79593368
1239 1239 a, g dbSNP:375377575
1243 1243 c, g, t dbSNP:755336024
1244 1244 c, g dbSNP:777957860
1247 1247 a, c dbSNP:41308303
1248 1248 g, t dbSNP:771316858
1271 1271 a, c dbSNP:779528536
1294 1294 a, g dbSNP:745362654
1298 1298 c, t dbSNP:541335259
1299 1299 a, c, g dbSNP:775157869
1303 1303 c, g dbSNP:768389200
1304 1304 a, c dbSNP:776260370
1310 1310 a, g dbSNP:371392768
1311 1311 c, t dbSNP:765072998
1317 1317 a, c, t dbSNP:139793542
1321 1321 a, g dbSNP:376031361
1328 1328 a, c dbSNP:767535362
1336 1336 c, g dbSNP:752803296
1352 1352 c, t dbSNP:760920254
1353 1353 a, c dbSNP:756295089
1354 1354 a, g dbSNP:778103654
1356 1356 c, t dbSNP:754135872
1358 1358 c, g dbSNP:753473056
1361 1361 a, c dbSNP:756726029
1375 1375 c, t dbSNP:778447396
1376 1376 a, g dbSNP:74873701
1414 1414 c, t dbSNP:145217327
1415 1415 a, g dbSNP:138259457
1439 1439 a, g dbSNP:746560774
1442 1442 a, g dbSNP:142385746
1446 1446 c, t dbSNP:776405072
1449 1449 a, g, t dbSNP:747892371
1452 1452 c, t dbSNP:773012684
1465 1465 a, t dbSNP:762836110
1476 1476 c, t dbSNP:766341369
1490 1490 c, g dbSNP:369682969
1502 1502 a, g dbSNP:201409177
1506 1506 a, g dbSNP:760295110
1518 1518 a, t dbSNP:560848217
1524 1524 c, t dbSNP:574105922
1531 1531 a, g dbSNP:770608742
1547 1547 c, t dbSNP:543408244
1567 1567 c, t dbSNP:746867194
1587 1587 a, g dbSNP:190770358
1589 1589 a, g dbSNP:775983590
1648 1648 a, c dbSNP:2168775
1691 1691 a, g dbSNP:547423635
1722 1722 g, t dbSNP:565859469
1745 1745 a, c dbSNP:34235595
1759 1759 -, a dbSNP:547262225
1788 1788 a, t dbSNP:76047934
1861 1861 a, c dbSNP:374845401
1864 1864 a, g dbSNP:541504060
1870 1870 c, t dbSNP:539475780
1896 1896 c, g dbSNP:140183311
1905 1905 a, t dbSNP:41311749
1910 1910 a, t dbSNP:537161509
1933 1933 a, g dbSNP:2290732
1974 1974 a, g dbSNP:372806449
1982 1982 g, t dbSNP:570515140
2030 2030 -, a dbSNP:113406057
2031 2031 -, a dbSNP:748975579
2031 2031 a, g dbSNP:80203789
2043 2043 c, g dbSNP:534982201
2093 2093 c, t dbSNP:578077816
2100 2100 a, g dbSNP:769792774
2102 2102 a, g dbSNP:187108662
2152 2152 g, t dbSNP:576074789
2192 2192 a, t dbSNP:574956160
2197 2197 a, t dbSNP:762913227
2269 2269 c, t dbSNP:542030537
2294 2294 c, t dbSNP:557546790
2308 2308 a, g dbSNP:576166615
2315 2315 c, t dbSNP:190302700
2344 2344 g, t dbSNP:141928377
2345 2345 a, g dbSNP:766942549
2391 2391 a, t dbSNP:564873207
2400 2400 a, c dbSNP:41315924
2409 2409 a, c dbSNP:139554086
2422 2422 g, t dbSNP:559414972
2434 2434 a, c dbSNP:369546620
2451 2451 a, g dbSNP:182930988
2472 2472 a, g dbSNP:142860832
2504 2504 a, t dbSNP:569376392
2507 2507 a, g dbSNP:563152013
2510 2510 a, g dbSNP:753466444
2524 2524 c, t dbSNP:374002978
2539 2539 c, t dbSNP:531272719
2546 2546 a, g dbSNP:752538724
2571 2571 a, g dbSNP:552298521
2642 2642 c, t dbSNP:377728451
2648 2648 c, t dbSNP:543428415
2716 2716 c, t dbSNP:549431469
2772 2772 a, g dbSNP:570502633
2812 2812 a, c dbSNP:534737930
2830 2830 c, t dbSNP:546995144
2844 2844 c, t dbSNP:187522588
2861 2861 c, t dbSNP:555430982
2884 2884 c, t dbSNP:749987275
2894 2894 a, t dbSNP:536156782
2896 2896 a, t dbSNP:557229266
2906 2906 a, g dbSNP:575959204
2911 2911 c, t dbSNP:193210849
2919 2919 a, g dbSNP:528083481
2959 2959 c, t dbSNP:558722133
2962 2962 a, g dbSNP:546239191
2969 2969 a, c dbSNP:998754
2989 2989 a, g dbSNP:540638714
3011 3011 a, g dbSNP:559313551
3105 3105 c, g dbSNP:746881638
3111 3111 c, g dbSNP:574602367
3116 3116 a, t dbSNP:746582124
3160 3160 a, g dbSNP:75337618
3187 3187 c, t dbSNP:563139650
3215 3215 g, t dbSNP:41303356
3220 3220 a, c dbSNP:151076324
3257 3257 c, g dbSNP:77720837
3258 3258 c, t dbSNP:75746434
3284 3284 g, t dbSNP:10059108
3286 3286 -, t dbSNP:770566138
3333 3333 c, t dbSNP:564215168
3359 3359 a, t dbSNP:368099176
3392 3392 c, t dbSNP:2290733
3412 3412 a, t dbSNP:41311747
3418 3418 c, t dbSNP:376130796
3459 3459 c, t dbSNP:770097153
3472 3472 c, g dbSNP:139565984
3482 3482 a, g dbSNP:535774149
3498 3498 a, g dbSNP:73317010
3535 3535 g, t dbSNP:569460050
3549 3549 c, t dbSNP:773306587
3557 3557 g, t dbSNP:540069540
3561 3561 c, t dbSNP:147837301
3588 3588 g, t dbSNP:189237787
3600 3600 c, t dbSNP:534722665
3603 3603 a, c dbSNP:538462336
3622 3622 -, t dbSNP:149385481
3650 3650 a, g dbSNP:552647468
3653 3653 g, t dbSNP:192225130
3677 3677 a, g dbSNP:141464277
3679 3679 g, t dbSNP:556870416
3742 3742 a, g dbSNP:575186628
3747 3747 a, g dbSNP:545561711
3751 3751 -, c dbSNP:35777559
3784 3784 a, c dbSNP:563948843
3863 3863 a, g dbSNP:528531605
3888 3888 a, g dbSNP:540771612
3927 3927 a, g dbSNP:536520286
3940 3940 g, t dbSNP:554920205
3978 3978 c, t dbSNP:561930118
3986 3986 a, c dbSNP:567624810
3994 3994 a, c dbSNP:774807571

Target ORF information:

RefSeq Version NM_001127645
Organism Homo sapiens (human)
Definition Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127645

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26115
Accession Version NM_001127648.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product gamma-aminobutyric acid receptor subunit alpha-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334834.1, DA232066.1, X14766.1 and AC021079.4. Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (7) differs in the 5' UTR, compared to variant 1. Variants 1-7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC030696.1, AK289792.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121120.1
CDS115..1485
Misc Feature(1)52..54(+)
Misc Feature(2)154..1440(+)
Misc Feature(3)241..864(+)
Misc Feature(4)868..933(+)
Misc Feature(5)883..1431(+)
Misc Feature(6)949..1014(+)
Misc Feature(7)1051..1116(+)
Misc Feature(8)1378..1443(+)
Exon (1)1..188
Gene:GABRA1
Gene Synonym:
Exon (2)189..301
Gene:GABRA1
Gene Synonym:
Exon (3)302..369
Gene:GABRA1
Gene Synonym:
Exon (4)370..590
Gene:GABRA1
Gene Synonym:
Exon (5)591..673
Gene:GABRA1
Gene Synonym:
Exon (6)674..817
Gene:GABRA1
Gene Synonym:
Exon (7)818..970
Gene:GABRA1
Gene Synonym:
Exon (8)971..1173
Gene:GABRA1
Gene Synonym:
Exon (9)1174..4022
Gene:GABRA1
Gene Synonym:
Translation
Position Chain Variation Link
17 17 c, t dbSNP:551643334
40 40 a, g dbSNP:772119155
56 56 a, g dbSNP:775265168
58 58 c, t dbSNP:760543537
61 61 c, t dbSNP:764113851
63 63 a, g dbSNP:750429173
70 70 a, g, t dbSNP:763132854
71 71 c, t dbSNP:751898261
73 73 c, t dbSNP:377647085
75 75 g, t dbSNP:200321984
77 77 a, g, t dbSNP:534129599
78 78 a, c dbSNP:778388081
80 80 c, t dbSNP:555933670
91 91 a, g dbSNP:770530140
101 101 g, t dbSNP:778424128
105 105 c, g dbSNP:376699435
111 111 c, t dbSNP:375475234
112 112 a, g dbSNP:574411082
114 114 a, g, t dbSNP:376266596
122 122 a, g dbSNP:111452646
132 132 c, t dbSNP:557682926
141 141 a, c, t dbSNP:113886269
146 146 c, t dbSNP:762939760
147 147 c, t dbSNP:766418049
154 154 c, t dbSNP:774587445
159 159 c, g dbSNP:759784427
160 160 c, t dbSNP:767887700
167 167 c, t dbSNP:779065852
168 168 c, g dbSNP:753085546
173 173 c, t dbSNP:756553428
182 182 c, g dbSNP:199819387
189 189 a, c, t dbSNP:75423500
192 192 c, t dbSNP:779666888
199 199 c, t dbSNP:143815396
200 200 c, t dbSNP:200218956
201 201 a, g dbSNP:374399356
207 207 a, c dbSNP:747927213
208 208 a, c dbSNP:769743354
210 210 a, g dbSNP:76224028
228 228 c, t dbSNP:543190682
266 266 a, g dbSNP:745932197
270 270 c, t dbSNP:1129647
273 273 c, t dbSNP:775714231
285 285 a, g dbSNP:760892094
289 289 a, c dbSNP:768778314
291 291 a, g dbSNP:777010469
297 297 a, g dbSNP:762124345
307 307 a, g dbSNP:11549458
312 312 c, g, t dbSNP:770208588
313 313 a, g dbSNP:763403354
315 315 a, g dbSNP:766903604
321 321 g, t dbSNP:751050781
330 330 c, t dbSNP:200716712
339 339 a, c dbSNP:547903306
345 345 c, t dbSNP:566224462
351 351 c, t dbSNP:536603724
366 366 c, t dbSNP:777649753
379 379 a, g dbSNP:146428720
384 384 c, t dbSNP:757165660
393 393 c, t dbSNP:267600528
402 402 c, t dbSNP:534779853
411 411 c, t dbSNP:750394365
412 412 a, g dbSNP:267600529
417 417 a, g dbSNP:758360317
419 419 g, t dbSNP:781084915
442 442 a, g dbSNP:552868295
449 449 a, g dbSNP:587777308
478 478 c, t dbSNP:1129648
492 492 a, g dbSNP:756299135
499 499 c, t dbSNP:146134200
504 504 c, t dbSNP:778019249
506 506 a, g dbSNP:749642686
522 522 c, t dbSNP:771269673
532 532 a, g dbSNP:199689997
538 538 c, t dbSNP:746306844
543 543 c, t dbSNP:772653297
550 550 c, t dbSNP:775096518
553 553 c, t dbSNP:139163545
555 555 g, t dbSNP:190024862
572 572 c, t dbSNP:776482117
573 573 c, t dbSNP:761693717
614 614 c, t dbSNP:750728250
615 615 a, g dbSNP:200750234
632 632 -, c dbSNP:35223687
635 635 -, c dbSNP:138145692
643 643 a, g dbSNP:780700439
645 645 a, c dbSNP:747452052
654 654 c, t dbSNP:769299843
657 657 a, c dbSNP:780800247
660 660 a, g dbSNP:747735903
697 697 a, g dbSNP:761791288
716 716 a, g dbSNP:765292293
717 717 c, g dbSNP:773177131
725 725 a, g dbSNP:777320447
726 726 c, t dbSNP:374616425
747 747 c, t dbSNP:587780947
754 754 a, c dbSNP:727503940
769 769 a, g dbSNP:587777364
773 773 g, t dbSNP:755451924
774 774 c, t dbSNP:767907251
778 778 a, g dbSNP:753182955
780 780 a, g dbSNP:756594849
786 786 a, g dbSNP:372742479
791 791 a, g dbSNP:748783999
813 813 c, t dbSNP:756876744
815 815 c, t dbSNP:778581160
825 825 c, t dbSNP:774587794
839 839 c, t dbSNP:759786297
843 843 c, t dbSNP:772218052
865 865 a, g dbSNP:587777307
870 870 a, c, t dbSNP:775895535
887 887 a, c dbSNP:764645086
896 896 a, g dbSNP:754406560
936 936 c, t dbSNP:142477850
945 945 a, g dbSNP:764666718
948 948 c, t dbSNP:749956729
954 954 a, g dbSNP:150497014
960 960 a, g dbSNP:139467768
965 965 c, t dbSNP:794727962
979 979 a, t dbSNP:189199636
987 987 c, g, t dbSNP:777911034
1000 1000 c, t dbSNP:149677108
1001 1001 a, t dbSNP:532607986
1022 1022 c, t dbSNP:267600530
1031 1031 a, c dbSNP:587777309
1058 1058 g, t dbSNP:747138999
1068 1068 c, t dbSNP:551045474
1074 1074 c, t dbSNP:761921247
1079 1079 a, c dbSNP:121434579
1080 1080 c, t dbSNP:201133616
1086 1086 g, t dbSNP:145617915
1090 1090 c, t dbSNP:527416473
1113 1113 a, t dbSNP:748550764
1122 1122 c, t dbSNP:200228901
1122 1122 -, t dbSNP:778743668
1133 1133 a, g dbSNP:773775590
1140 1140 c, t dbSNP:750925935
1184 1184 a, t dbSNP:749707253
1192 1192 c, t dbSNP:771498937
1193 1193 a, c dbSNP:80337021
1200 1200 c, t dbSNP:187648029
1209 1209 c, t dbSNP:558548302
1218 1218 c, t dbSNP:771832995
1219 1219 a, g, t dbSNP:775344663
1226 1226 c, t dbSNP:372570305
1236 1236 c, t dbSNP:370816784
1241 1241 a, c dbSNP:761617651
1242 1242 c, g dbSNP:765261463
1244 1244 c, t dbSNP:751571034
1251 1251 a, g dbSNP:755109332
1255 1255 a, c dbSNP:371470198
1260 1260 a, c, t dbSNP:79593368
1261 1261 a, g dbSNP:375377575
1265 1265 c, g, t dbSNP:755336024
1266 1266 c, g dbSNP:777957860
1269 1269 a, c dbSNP:41308303
1270 1270 g, t dbSNP:771316858
1293 1293 a, c dbSNP:779528536
1316 1316 a, g dbSNP:745362654
1320 1320 c, t dbSNP:541335259
1321 1321 a, c, g dbSNP:775157869
1325 1325 c, g dbSNP:768389200
1326 1326 a, c dbSNP:776260370
1332 1332 a, g dbSNP:371392768
1333 1333 c, t dbSNP:765072998
1339 1339 a, c, t dbSNP:139793542
1343 1343 a, g dbSNP:376031361
1350 1350 a, c dbSNP:767535362
1358 1358 c, g dbSNP:752803296
1374 1374 c, t dbSNP:760920254
1375 1375 a, c dbSNP:756295089
1376 1376 a, g dbSNP:778103654
1378 1378 c, t dbSNP:754135872
1380 1380 c, g dbSNP:753473056
1383 1383 a, c dbSNP:756726029
1397 1397 c, t dbSNP:778447396
1398 1398 a, g dbSNP:74873701
1436 1436 c, t dbSNP:145217327
1437 1437 a, g dbSNP:138259457
1461 1461 a, g dbSNP:746560774
1464 1464 a, g dbSNP:142385746
1468 1468 c, t dbSNP:776405072
1471 1471 a, g, t dbSNP:747892371
1474 1474 c, t dbSNP:773012684
1487 1487 a, t dbSNP:762836110
1498 1498 c, t dbSNP:766341369
1512 1512 c, g dbSNP:369682969
1524 1524 a, g dbSNP:201409177
1528 1528 a, g dbSNP:760295110
1540 1540 a, t dbSNP:560848217
1546 1546 c, t dbSNP:574105922
1553 1553 a, g dbSNP:770608742
1569 1569 c, t dbSNP:543408244
1589 1589 c, t dbSNP:746867194
1609 1609 a, g dbSNP:190770358
1611 1611 a, g dbSNP:775983590
1670 1670 a, c dbSNP:2168775
1713 1713 a, g dbSNP:547423635
1744 1744 g, t dbSNP:565859469
1767 1767 a, c dbSNP:34235595
1781 1781 -, a dbSNP:547262225
1810 1810 a, t dbSNP:76047934
1883 1883 a, c dbSNP:374845401
1886 1886 a, g dbSNP:541504060
1892 1892 c, t dbSNP:539475780
1918 1918 c, g dbSNP:140183311
1927 1927 a, t dbSNP:41311749
1932 1932 a, t dbSNP:537161509
1955 1955 a, g dbSNP:2290732
1996 1996 a, g dbSNP:372806449
2004 2004 g, t dbSNP:570515140
2052 2052 -, a dbSNP:113406057
2053 2053 -, a dbSNP:748975579
2053 2053 a, g dbSNP:80203789
2065 2065 c, g dbSNP:534982201
2115 2115 c, t dbSNP:578077816
2122 2122 a, g dbSNP:769792774
2124 2124 a, g dbSNP:187108662
2174 2174 g, t dbSNP:576074789
2214 2214 a, t dbSNP:574956160
2219 2219 a, t dbSNP:762913227
2291 2291 c, t dbSNP:542030537
2316 2316 c, t dbSNP:557546790
2330 2330 a, g dbSNP:576166615
2337 2337 c, t dbSNP:190302700
2366 2366 g, t dbSNP:141928377
2367 2367 a, g dbSNP:766942549
2413 2413 a, t dbSNP:564873207
2422 2422 a, c dbSNP:41315924
2431 2431 a, c dbSNP:139554086
2444 2444 g, t dbSNP:559414972
2456 2456 a, c dbSNP:369546620
2473 2473 a, g dbSNP:182930988
2494 2494 a, g dbSNP:142860832
2526 2526 a, t dbSNP:569376392
2529 2529 a, g dbSNP:563152013
2532 2532 a, g dbSNP:753466444
2546 2546 c, t dbSNP:374002978
2561 2561 c, t dbSNP:531272719
2568 2568 a, g dbSNP:752538724
2593 2593 a, g dbSNP:552298521
2664 2664 c, t dbSNP:377728451
2670 2670 c, t dbSNP:543428415
2738 2738 c, t dbSNP:549431469
2794 2794 a, g dbSNP:570502633
2834 2834 a, c dbSNP:534737930
2852 2852 c, t dbSNP:546995144
2866 2866 c, t dbSNP:187522588
2883 2883 c, t dbSNP:555430982
2906 2906 c, t dbSNP:749987275
2916 2916 a, t dbSNP:536156782
2918 2918 a, t dbSNP:557229266
2928 2928 a, g dbSNP:575959204
2933 2933 c, t dbSNP:193210849
2941 2941 a, g dbSNP:528083481
2981 2981 c, t dbSNP:558722133
2984 2984 a, g dbSNP:546239191
2991 2991 a, c dbSNP:998754
3011 3011 a, g dbSNP:540638714
3033 3033 a, g dbSNP:559313551
3127 3127 c, g dbSNP:746881638
3133 3133 c, g dbSNP:574602367
3138 3138 a, t dbSNP:746582124
3182 3182 a, g dbSNP:75337618
3209 3209 c, t dbSNP:563139650
3237 3237 g, t dbSNP:41303356
3242 3242 a, c dbSNP:151076324
3279 3279 c, g dbSNP:77720837
3280 3280 c, t dbSNP:75746434
3306 3306 g, t dbSNP:10059108
3308 3308 -, t dbSNP:770566138
3355 3355 c, t dbSNP:564215168
3381 3381 a, t dbSNP:368099176
3414 3414 c, t dbSNP:2290733
3434 3434 a, t dbSNP:41311747
3440 3440 c, t dbSNP:376130796
3481 3481 c, t dbSNP:770097153
3494 3494 c, g dbSNP:139565984
3504 3504 a, g dbSNP:535774149
3520 3520 a, g dbSNP:73317010
3557 3557 g, t dbSNP:569460050
3571 3571 c, t dbSNP:773306587
3579 3579 g, t dbSNP:540069540
3583 3583 c, t dbSNP:147837301
3610 3610 g, t dbSNP:189237787
3622 3622 c, t dbSNP:534722665
3625 3625 a, c dbSNP:538462336
3644 3644 -, t dbSNP:149385481
3672 3672 a, g dbSNP:552647468
3675 3675 g, t dbSNP:192225130
3699 3699 a, g dbSNP:141464277
3701 3701 g, t dbSNP:556870416
3764 3764 a, g dbSNP:575186628
3769 3769 a, g dbSNP:545561711
3773 3773 -, c dbSNP:35777559
3806 3806 a, c dbSNP:563948843
3885 3885 a, g dbSNP:528531605
3910 3910 a, g dbSNP:540771612
3949 3949 a, g dbSNP:536520286
3962 3962 g, t dbSNP:554920205
4000 4000 c, t dbSNP:561930118
4008 4008 a, c dbSNP:567624810
4016 4016 a, c dbSNP:774807571

Target ORF information:

RefSeq Version NM_001127648
Organism Homo sapiens (human)
Definition Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127648

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGGAAAA GTCCAGGTCT GTCTGACTGT CTTTGGGCCT GGATCCTCCT TCTGAGCACA 
CTGACTGGAA GAAGCTATGG ACAGCCGTCA TTACAAGATG AACTTAAAGA CAATACCACT
GTCTTCACCA GGATTTTGGA CAGACTCCTA GATGGTTATG ACAATCGCCT GAGACCAGGA
TTGGGAGAGC GTGTAACCGA AGTGAAGACT GATATCTTCG TCACCAGTTT CGGACCCGTT
TCAGACCATG ATATGGAATA TACAATAGAT GTATTTTTCC GTCAAAGCTG GAAGGATGAA
AGGTTAAAAT TTAAAGGACC TATGACAGTC CTCCGGTTAA ATAACCTAAT GGCAAGTAAA
ATCTGGACTC CGGACACATT TTTCCACAAT GGAAAGAAGT CAGTGGCCCA CAACATGACC
ATGCCCAACA AACTCCTGCG GATCACAGAG GATGGCACCT TGCTGTACAC CATGAGGCTG
ACAGTGAGAG CTGAATGTCC GATGCATTTG GAGGACTTCC CTATGGATGC CCATGCTTGC
CCACTAAAAT TTGGAAGTTA TGCTTATACA AGAGCAGAAG TTGTTTATGA ATGGACCAGA
GAGCCAGCAC GCTCAGTGGT TGTAGCAGAA GATGGATCAC GTCTAAACCA GTATGACCTT
CTTGGACAAA CAGTAGACTC TGGAATTGTC CAGTCAAGTA CAGGAGAATA TGTTGTTATG
ACCACTCATT TCCACTTGAA GAGAAAGATT GGCTACTTTG TTATTCAAAC ATACCTGCCA
TGCATAATGA CAGTGATTCT CTCACAAGTC TCCTTCTGGC TCAACAGAGA GTCTGTACCA
GCAAGAACTG TCTTTGGAGT AACAACTGTG CTCACCATGA CAACATTGAG CATCAGTGCC
AGAAACTCCC TCCCTAAGGT GGCTTATGCA ACAGCTATGG ATTGGTTTAT TGCCGTGTGC
TATGCCTTTG TGTTCTCAGC TCTGATTGAG TTTGCCACAG TAAACTATTT CACTAAGAGA
GGTTATGCAT GGGATGGCAA AAGTGTGGTT CCAGAAAAGC CAAAGAAAGT AAAGGATCCT
CTTATTAAGA AAAACAACAC TTACGCTCCA ACAGCAACCA GCTACACCCC TAATTTGGCC
AGGGGCGACC CGGGCTTAGC CACCATTGCT AAAAGTGCAA CCATAGAACC TAAAGAGGTC
AAGCCCGAAA CAAAACCACC AGAACCCAAG AAAACCTTTA ACAGTGTCAG CAAAATTGAC
CGACTGTCAA GAATAGCCTT CCCGCTGCTA TTTGGAATCT TTAACTTAGT CTACTGGGCT
ACGTATTTAA ACAGAGAGCC TCAGCTAAAA GCCCCCACAC CACATCAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.