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PTF1A cDNA ORF clone, Homo sapiens (human)

Gene Symbol PTF1A
Entrez Gene ID 256297
Full Name pancreas specific transcription factor, 1a
Synonyms PACA, PAGEN2, PTF1-p48, bHLHa29
General protein information
Preferred Names
pancreas transcription factor 1 subunit alpha
Names
pancreas transcription factor 1 subunit alpha
class II bHLH protein PTF1A
bHLH transcription factor p48
class A basic helix-loop-helix protein 29
pancreas-specific transcription factor 1a
exocrine pancreas-specific transcription factor p48
p48 DNA-binding subunit of transcription factor PTF1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10p12.2

Summary This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

607194

Disorder Html: Diabetes mellitus, permanent neonatal, with cerebellar agenesis,

mRNA and Protein(s)

mRNA Protein Name
NM_178161 NP_835455 pancreas transcription factor 1 subunit alpha



Homo sapiens (human) PTF1A NP_835455.1
Pan troglodytes (chimpanzee) PTF1A XP_001146416.2
Canis lupus familiaris (dog) PTF1A XP_850386.2
Bos taurus (cattle) PTF1A XP_002707767.1
Mus musculus (house mouse) Ptf1a NP_061279.2
Rattus norvegicus (Norway rat) Ptf1a NP_446416.1
Danio rerio (zebrafish) ptf1a NP_997524.1
Xenopus (Silurana) tropicalis (western clawed frog) ptf1a XP_002933181.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19480
NM_178161 Homo sapiens pancreas specific transcription factor, 1a (PTF1A), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19480
Clone ID Related Accession (Same CDS sequence) NM_178161
Accession Version NM_178161.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 987bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pancreas transcription factor 1 subunit alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139281.7, BM055347.1 and CK904916.1. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2006 this sequence version replaced gi:30039709. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK904917.1, BM055347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACGCGG TGTTGCTGGA GCACTTCCCC GGGGGCCTAG ACGCCTTTCC TTCTTCGTAC 
TTCGACGAGG ACGACTTCTT CACCGACCAG TCTTCACGGG ACCCCCTGGA GGACGGCGAT
GAGCTGCTGG CGGACGAGCA GGCCGAGGTG GAGTTCCTTA GCCACCAGCT CCACGAGTAC
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAGCCCGCGC CCCCGGCCGC CCCGCTAGCG
CTCGCCCCGC CGTCCTCGGG GGGCCTCGGT GAGCCAGACG ACGGCGGCGG CGGCGGCTAC
TGCTGCGAGA CGGGGGCGCC CCCAGGCGGC TTCCCCTACT CGCCCGGCTC GCCGCCCTCG
TGCCTGGCCT ACCCGTGCGC CGGGGCGGCA GTACTGTCTC CCGGGGCGCG GCTGCGCGGC
CTGAGCGGAG CGGCGGCTGC GGCGGCGCGG CGCCGGCGGC GGGTGCGCTC CGAGGCGGAG
CTGCAGCAGC TGCGGCAGGC GGCCAACGTG CGCGAGCGGC GGCGCATGCA GTCCATCAAC
GACGCCTTCG AGGGGCTGCG CTCGCACATC CCCACGCTGC CCTACGAGAA GCGCCTCTCC
AAGGTGGACA CGCTGCGCCT GGCCATCGGC TACATCAACT TCCTCAGCGA GCTCGTGCAG
GCCGACCTGC CCTTGCGCGG CGGTGGCGCG GGCGGCTGCG GGGGGCCGGG CGGCGGCGGG
CGCCTGGGCG GGGACAGCCC GGGCAGCCAG GCCCAGAAGG TCATCATCTG CCATCGGGGC
ACCCGGTCCC CCTCCCCCAG CGACCCTGAT TATGGCCTCC CTCCCCTAGC AGGACACTCT
CTCTCATGGA CTGATGAAAA ACAACTCAAG GAACAAAATA TTATCCGAAC AGCCAAAGTC
TGGACCCCAG AGGACCCCAG AAAACTCAAC AGCAAATCTT CCTTCAACAA CATAGAAAAC
GAACCACCAT TTGAGTTTGT GTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_835455.1
CDS1..987
Misc Feature(1)493..660(+)
Misc Feature(2)493..603(+)
Misc Feature(3)514..516(+)
Misc Feature(4)532..657(+)
Exon (1)1..784
Gene:PTF1A
Gene Synonym:
Exon (2)785..1333
Gene:PTF1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_178161
Organism Homo sapiens (human)
Definition Homo sapiens pancreas specific transcription factor, 1a (PTF1A), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_178161

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACGCGG TGTTGCTGGA GCACTTCCCC GGGGGCCTAG ACGCCTTTCC TTCTTCGTAC 
TTCGACGAGG ACGACTTCTT CACCGACCAG TCTTCACGGG ACCCCCTGGA GGACGGCGAT
GAGCTGCTGG CGGACGAGCA GGCCGAGGTG GAGTTCCTTA GCCACCAGCT CCACGAGTAC
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAGCCCGCGC CCCCGGCCGC CCCGCTAGCG
CTCGCCCCGC CGTCCTCGGG GGGCCTCGGT GAGCCAGACG ACGGCGGCGG CGGCGGCTAC
TGCTGCGAGA CGGGGGCGCC CCCAGGCGGC TTCCCCTACT CGCCCGGCTC GCCGCCCTCG
TGCCTGGCCT ACCCGTGCGC CGGGGCGGCA GTACTGTCTC CCGGGGCGCG GCTGCGCGGC
CTGAGCGGAG CGGCGGCTGC GGCGGCGCGG CGCCGGCGGC GGGTGCGCTC CGAGGCGGAG
CTGCAGCAGC TGCGGCAGGC GGCCAACGTG CGCGAGCGGC GGCGCATGCA GTCCATCAAC
GACGCCTTCG AGGGGCTGCG CTCGCACATC CCCACGCTGC CCTACGAGAA GCGCCTCTCC
AAGGTGGACA CGCTGCGCCT GGCCATCGGC TACATCAACT TCCTCAGCGA GCTCGTGCAG
GCCGACCTGC CCTTGCGCGG CGGTGGCGCG GGCGGCTGCG GGGGGCCGGG CGGCGGCGGG
CGCCTGGGCG GGGACAGCCC GGGCAGCCAG GCCCAGAAGG TCATCATCTG CCATCGGGGC
ACCCGGTCCC CCTCCCCCAG CGACCCTGAT TATGGCCTCC CTCCCCTAGC AGGACACTCT
CTCTCATGGA CTGATGAAAA ACAACTCAAG GAACAAAATA TTATCCGAAC AGCCAAAGTC
TGGACCCCAG AGGACCCCAG AAAACTCAAC AGCAAATCTT CCTTCAACAA CATAGAAAAC
GAACCACCAT TTGAGTTTGT GTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Recessive mutations in a distal PTF1A enhancer cause isolated pancreatic agenesis
Nat. Genet. 46 (1), 61-64 (2014)
Weedon MN, Cebola I, Patch AM, Flanagan SE, De Franco E, Caswell R, Rodriguez-Segui SA, Shaw-Smith C, Cho CH, Lango Allen H, Houghton JA, Roth CL, Chen R, Hussain K, Marsh P, Vallier L, Murray A, Ellard S, Ferrer J and Hattersley AT.


book

ICAT is a novel Ptf1a interactor that regulates pancreatic acinar differentiation and displays altered expression in tumours
Biochem. J. 451 (3), 395-405 (2013)
Campos ML, Sanchez-Arevalo Lobo VJ, Rodolosse A, Gottardi CJ, Mafficini A, Beghelli S, Scardoni M, Bassi C, Scarpa A and Real FX.


book

Genetic lineage tracing, a powerful tool to investigate the embryonic organogenesis and adult organ maintenance of the pancreas
J Hepatobiliary Pancreat Sci 18 (1), 1-5 (2011)
Kawaguchi Y, Takaori K and Uemoto S.


book

Multiple transcriptional mechanisms control Ptf1a levels during neural development including autoregulation by the PTF1-J complex
J. Neurosci. 29 (36), 11139-11148 (2009)
Meredith DM, Masui T, Swift GH, MacDonald RJ and Johnson JE.


book

A Turkish newborn infant with cerebellar agenesis/neonatal diabetes mellitus and PTF1A mutation
Genet. Couns. 20 (2), 147-152 (2009)
Tutak E, Satar M, Yapicioglu H, Altintas A, Narli N, Herguner O and Bayram Y.


book

The bHLH protein PTF1-p48 is essential for the formation of the exocrine and the correct spatial organization of the endocrine pancreas
Genes Dev. 12 (23), 3752-3763 (1998)
Krapp A, Knofler M, Ledermann B, Burki K, Berney C, Zoerkler N, Hagenbuchle O and Wellauer PK.


book

Constitutive expression of the gene for the cell-specific p48 DNA-binding subunit of pancreas transcription factor 1 in cultured cells is under control of binding sites for transcription factors Sp1 and alphaCbf
J. Biol. Chem. 271 (36), 21993-22002 (1996)
Knofler M, Krapp A, Hagenbuchle O and Wellauer PK.


book

The p48 DNA-binding subunit of transcription factor PTF1 is a new exocrine pancreas-specific basic helix-loop-helix protein
EMBO J. 15 (16), 4317-4329 (1996)
Krapp A, Knofler M, Frutiger S, Hughes GJ, Hagenbuchle O and Wellauer PK.


book

Nuclear targeting of the transcription factor PTF1 is mediated by a protein subunit that does not bind to the PTF1 cognate sequence
Cell 67 (5), 987-994 (1991)
Sommer L, Hagenbuchle O, Wellauer PK and Strubin M.


book

The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA
Genes Dev. 3 (10), 1613-1624 (1989)
Roux E, Strubin M, Hagenbuchle O and Wellauer PK.


 
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