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MFSD8 major facilitator superfamily domain containing 8 [Homo sapiens (human)]

Gene Symbol MFSD8
Entrez Gene ID 256471
Full Name major facilitator superfamily domain containing 8
Synonyms CCMD, CLN7
General protein information
Preferred Names
major facilitator superfamily domain-containing protein 8
Names
major facilitator superfamily domain-containing protein 8
ceroid-lipofuscinosis neuronal protein 7
ceroid-lipofuscinosis, neuronal 7, late infantile
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q28.2

Summary This gene encodes a ubiquitous integral membrane protein that contains a transporter domain and a major facilitator superfamily (MFS) domain. Other members of the major facilitator superfamily transport small solutes through chemiosmotic ion gradients. The substrate transported by this protein is unknown. The protein likely localizes to lysosomal membranes. Mutations in this gene are correlated with a variant form of late infantile-onset neuronal ceroid lipofuscinoses (vLINCL). [provided by RefSeq, Oct 2008]. lac of sum
Disorder MIM:

611124

Disorder Html: Ceroid lipofuscinosis, neuronal, 7, 610951 (3)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu26686 XM_005262893 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu78398 XM_011531830 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu38845 XM_005262896 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu38846 XM_005262897 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu78399 XM_011531831 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu38847 XM_005262898 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu78400 XM_011531832 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu38848 XM_005262900 PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu26686 NM_152778 Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu26686D
Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product major facilitator superfamily domain-containing protein 8 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAGA TTGGCGAGCG TGCTATTCTA
CTGGGAGGAC TCATCGTTGT ATGGGTTGGC TTCTTTATCT TGTTACCTTG GGGAAATCAA
TTTCCCAAAA TACAGTGGGA AGATTTGCAC AATAATTCAA TCCCTAATAC CACATTTGGG
GAAATTATTA TTGGTCTTTG GAAGTCTCCA ATGGAAGATG ACAATGAAAG ACCAACTGGT
TGCTCGATTG AACAAGCCTG GTGCCTCTAC ACCCCGGTGA TTCATCTGGC CCAGTTCCTT
ACATCAGCTG TGCTAATAGG ATTAGGCTAT CCAGTCTGCA ATCTTATGTC CTATACTCTA
TATTCAAAAA TTCTAGGACC AAAACCTCAG GGTGTATACA TGGGCTGGTT AACAGCATCT
GGAAGTGGAG CCCGGATTCT TGGGCCTATG TTCATCAGCC AAGTGTATGC TCACTGGGGA
CCACGATGGG CATTCAGCCT GGTGTGTGGA ATAATAGTGC TCACCATCAC CCTCCTGGGA
GTGGTTTACA AAAGACTCAT TGCTCTTTCT GTAAGATATG GGAGGATTCA GGAATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005262950.1
CDS78..1634
Misc Feature(1)192..1586(+)
Misc Feature(2)201..1484(+)
Misc Feature(3)234..1472(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:532558333
23 23 c, g dbSNP:568845783
27 27 a, c dbSNP:760736619
35 35 a, g dbSNP:775119203
39 39 c, g dbSNP:374912082
41 41 a, t dbSNP:369032119
47 47 c, g dbSNP:771730847
50 50 a, c, t dbSNP:550627957
51 51 a, c, g, t dbSNP:375020549
56 56 a, g dbSNP:769126800
57 57 c, g, t dbSNP:200352565
58 58 a, g, t dbSNP:761604533
59 59 c, t dbSNP:750582324
60 60 c, t dbSNP:779186294
65 65 a, g dbSNP:757490861
67 67 a, g dbSNP:753641515
70 70 a, g dbSNP:763846149
71 71 c, t dbSNP:760642574
73 73 a, t dbSNP:752714009
77 77 c, t dbSNP:767069868
84 84 a, c, g dbSNP:773799172
91 91 c, g dbSNP:139409959
92 92 a, g dbSNP:762559863
93 93 a, c dbSNP:777004095
95 95 c, g dbSNP:771879274
99 99 a, g dbSNP:747446547
100 100 a, g dbSNP:780548594
102 102 g, t dbSNP:772629400
106 106 a, g dbSNP:532843679
108 108 a, g dbSNP:778902510
111 111 c, t dbSNP:371882083
112 112 c, t dbSNP:749496400
114 114 a, c dbSNP:150892838
117 117 g, t dbSNP:755977683
123 123 c, g dbSNP:752624076
128 128 a, c dbSNP:767558098
140 140 a, c dbSNP:201926015
143 143 a, t dbSNP:145529594
148 148 a, c dbSNP:761497836
149 149 c, t dbSNP:775812921
151 151 g, t dbSNP:767893733
154 154 g, t dbSNP:760049336
155 155 a, c, g dbSNP:770986122
179 179 c, t dbSNP:139212190
180 180 c, t dbSNP:749315686
181 181 a, g dbSNP:146596875
183 183 c, t dbSNP:200001979
192 192 a, g, t dbSNP:201739608
193 193 a, t dbSNP:140349255
196 196 c, g dbSNP:768336733
209 209 g, t dbSNP:746904557
230 230 a, g dbSNP:587781037
231 231 a, g dbSNP:779838200
233 233 a, g, t dbSNP:753433200
239 239 g, t dbSNP:777277246
248 248 a, g dbSNP:755862714
252 252 c, t dbSNP:751847064
254 254 c, t dbSNP:766788326
255 255 a, g dbSNP:143902749
256 256 c, t dbSNP:763512187
263 263 a, c dbSNP:750969105
268 268 c, t dbSNP:113960067
275 275 a, g dbSNP:765347608
276 276 a, g dbSNP:757876846
283 283 c, t dbSNP:147750747
284 284 a, g dbSNP:781051390
286 286 c, t dbSNP:570440254
293 293 c, t dbSNP:760772807
295 295 a, c dbSNP:377555060
296 296 a, g dbSNP:143654391
298 298 a, g dbSNP:759313071
303 303 c, t dbSNP:774200444
312 312 c, g, t dbSNP:749244700
333 333 a, g dbSNP:552923962
347 347 a, c, t dbSNP:754809138
354 354 a, g dbSNP:578102641
377 377 c, t dbSNP:754739634
383 383 a, g dbSNP:746201253
386 386 a, t dbSNP:148916499
391 391 a, g dbSNP:751274078
393 393 c, t dbSNP:757793193
396 396 c, t dbSNP:754403098
397 397 c, t dbSNP:764017632
403 403 g, t dbSNP:11732377
409 409 c, t dbSNP:145633957
419 419 c, t dbSNP:201905099
420 420 a, g dbSNP:183450731
427 427 c, t dbSNP:759795397
430 430 a, g dbSNP:774112195
435 435 c, g dbSNP:144845312
439 439 a, c, g dbSNP:118203978
449 449 c, t dbSNP:141031338
453 453 a, g dbSNP:773214617
459 459 c, g dbSNP:557910536
463 463 c, t dbSNP:769677706
466 466 a, g dbSNP:761377902
477 477 c, t dbSNP:776316095
480 480 a, g dbSNP:768398882
486 486 a, g dbSNP:146479250
488 488 -, tgctcgt dbSNP:765061912
488 488 c, t dbSNP:201652718
493 493 a, g, t dbSNP:749704755
498 498 c, t dbSNP:778330834
505 505 a, c, g dbSNP:762496121
512 512 a, c dbSNP:781299975
513 513 a, g dbSNP:755152088
522 522 a, g dbSNP:780249947
525 525 a, g dbSNP:758584752
530 530 c, t dbSNP:750205976
542 542 c, t dbSNP:753346740
570 570 a, c dbSNP:761767969
575 575 a, g dbSNP:753834794
577 577 a, g dbSNP:763642606
581 581 c, t dbSNP:760252298
583 583 c, t dbSNP:775207180
591 591 a, g dbSNP:540305093
602 602 c, t dbSNP:556661896
608 608 a, g dbSNP:376733427
610 610 c, t dbSNP:201368623
618 618 a, t dbSNP:770057458
628 628 c, t dbSNP:201976338
632 632 g, t dbSNP:562103818
640 640 c, t dbSNP:766111411
650 650 a, g dbSNP:115275192
653 653 c, g dbSNP:777020801
665 665 a, g dbSNP:769124699
667 667 a, c, g, t dbSNP:28544073
667 667 -, g dbSNP:776253867
669 669 a, g dbSNP:377029630
676 676 g, t dbSNP:558274487
681 681 a, g dbSNP:757623473
698 698 a, g dbSNP:749064699
708 708 a, g dbSNP:777761765
711 711 a, g dbSNP:756069266
729 729 a, g dbSNP:368614789
731 731 c, t dbSNP:766992766
733 733 c, t dbSNP:112734134
735 735 c, g dbSNP:142063476
737 737 g, t dbSNP:765916112
754 754 c, t dbSNP:200591471
758 758 g, t dbSNP:754149229
761 761 c, g dbSNP:764470493
763 763 g, t dbSNP:761101343
766 766 c, t dbSNP:375681665
780 780 c, t dbSNP:779558035
783 783 c, t dbSNP:758002981
784 784 a, c, g dbSNP:371250204
796 796 c, g dbSNP:761017363
803 803 a, g dbSNP:753174925
806 806 g, t dbSNP:557451270
821 821 g, t dbSNP:199555198
844 844 a, c dbSNP:763119554
851 851 a, g dbSNP:773465316
864 864 a, g dbSNP:374234685
886 886 c, t dbSNP:200284184
888 888 a, g dbSNP:762117279
889 889 c, t dbSNP:776245759
890 890 c, t dbSNP:768499490
894 894 a, g dbSNP:144848697
895 895 a, g dbSNP:143288262
896 896 c, t dbSNP:772066492
897 897 a, g dbSNP:745354809
898 898 c, t dbSNP:778448039
903 903 g, t dbSNP:756815122
908 908 -, t dbSNP:775699005
915 915 c, g dbSNP:749001842
917 917 a, g dbSNP:199982542
923 923 a, c dbSNP:776272077
940 940 c, t dbSNP:755384900
951 951 c, t dbSNP:200631121
953 953 a, g dbSNP:770483205
958 958 a, c, t dbSNP:140948465
963 963 a, g dbSNP:147295085
971 971 c, g, t dbSNP:118203977
974 974 c, t dbSNP:747352043
981 981 c, g dbSNP:780583913
988 988 a, g dbSNP:371333586
990 990 a, g dbSNP:750969401
994 994 c, t dbSNP:200971312
1000 1000 a, g dbSNP:757345902
1006 1006 a, g dbSNP:118203975
1008 1008 a, g dbSNP:753930474
1011 1011 a, g dbSNP:77098161
1012 1012 c, t dbSNP:556875684
1015 1015 c, t dbSNP:752441316
1021 1021 a, c dbSNP:767331120
1036 1036 c, t dbSNP:759406697
1037 1037 c, t dbSNP:376954943
1038 1038 a, g dbSNP:76506918
1040 1040 a, t dbSNP:762491362
1042 1042 c, t dbSNP:772644224
1052 1052 a, g dbSNP:570989221
1055 1055 a, g dbSNP:138219028
1082 1082 a, c, t dbSNP:772735057
1083 1083 c, g dbSNP:150418024
1084 1084 a, c dbSNP:761499299
1086 1086 c, t dbSNP:373923336
1087 1087 a, g dbSNP:200386040
1099 1099 c, t dbSNP:768488730
1112 1112 c, t dbSNP:141422574
1113 1113 a, g dbSNP:746209973
1116 1116 c, g dbSNP:774629940
1118 1118 a, g dbSNP:148291156
1134 1134 a, g dbSNP:749791052
1138 1138 c, t dbSNP:777843146
1143 1143 a, c dbSNP:756204684
1145 1145 c, t dbSNP:376258320
1149 1149 a, g dbSNP:781391960
1151 1151 a, g dbSNP:755236045
1157 1157 a, g dbSNP:751316775
1179 1179 c, g dbSNP:727502800
1184 1184 c, g dbSNP:747197852
1192 1192 a, g dbSNP:779835846
1193 1193 a, t dbSNP:758114648
1208 1208 c, t dbSNP:760689362
1212 1212 a, t dbSNP:750297992
1213 1213 c, t dbSNP:191172038
1216 1216 a, g dbSNP:757306822
1218 1218 g, t dbSNP:724159970
1228 1228 c, t dbSNP:763730339
1230 1230 c, g dbSNP:11098943
1241 1241 g, t dbSNP:775018656
1245 1245 c, g dbSNP:767262517
1248 1248 a, t dbSNP:763226224
1251 1251 a, g dbSNP:773610115
1254 1254 a, g dbSNP:537799887
1255 1255 a, t dbSNP:762336516
1257 1257 a, g, t dbSNP:768627011
1269 1269 c, t dbSNP:747170947
1282 1282 c, t dbSNP:200745039
1283 1283 a, g dbSNP:772377066
1285 1285 c, t dbSNP:745633928
1290 1290 a, c dbSNP:778875017
1291 1291 a, g dbSNP:757222651
1295 1295 c, t dbSNP:753832653
1305 1305 c, t dbSNP:777361767
1308 1308 a, g dbSNP:755570201
1312 1312 c, t dbSNP:267607235
1313 1313 a, g dbSNP:767048437
1330 1330 a, g dbSNP:759285288
1332 1332 a, t dbSNP:748707366
1334 1334 c, t dbSNP:750743360
1339 1339 c, t dbSNP:765587961
1343 1343 a, g dbSNP:762246290
1345 1345 c, t dbSNP:3733319
1355 1355 a, g dbSNP:769272117
1357 1357 a, g dbSNP:760640742
1363 1363 a, g dbSNP:118203976
1364 1364 c, g, t dbSNP:138072045
1388 1388 c, g dbSNP:145453022
1393 1393 c, t dbSNP:778785216
1397 1397 a, g dbSNP:148864710
1399 1399 a, g dbSNP:749153763
1404 1404 a, g dbSNP:777570875
1405 1405 a, t dbSNP:756121904
1412 1412 a, g dbSNP:145518709
1422 1422 c, t dbSNP:371428597
1423 1423 c, t dbSNP:780779080
1434 1434 c, t dbSNP:769573477
1437 1437 a, g dbSNP:748118750
1438 1438 c, t dbSNP:559155109
1449 1449 a, g dbSNP:754467879
1454 1454 a, g dbSNP:185047041
1467 1467 a, g dbSNP:779648626
1468 1468 c, t dbSNP:758095101
1476 1476 a, c dbSNP:754100928
1485 1485 a, g dbSNP:764549054
1497 1497 c, g dbSNP:370663969
1500 1500 c, g dbSNP:528664888
1508 1508 g, t dbSNP:767966853
1521 1521 c, t dbSNP:724159971
1522 1522 a, c, g dbSNP:547726489
1535 1535 c, g dbSNP:766690694
1546 1546 a, g dbSNP:561194518
1552 1552 c, t dbSNP:183448311
1557 1557 c, t dbSNP:149683848
1571 1571 c, t dbSNP:769642042
1573 1573 -, tgggagtg dbSNP:756701118
1579 1579 c, t dbSNP:747948366
1585 1585 a, g dbSNP:780161755
1590 1590 a, c dbSNP:776622815
1593 1593 c, g dbSNP:570757797
1595 1595 c, t dbSNP:140732035
1598 1598 g, t dbSNP:779560159
1606 1606 c, t dbSNP:758005057
1611 1611 a, g dbSNP:745541140
1626 1626 c, g dbSNP:777943565
1635 1635 a, c, g dbSNP:753071746
1637 1637 c, t dbSNP:374487025
1657 1657 a, t dbSNP:766561103
1667 1667 a, g dbSNP:751643210
1672 1672 a, g dbSNP:766600653
1678 1678 a, c dbSNP:763024313
1680 1680 c, t dbSNP:750641784
1682 1682 g, t dbSNP:765439882
1690 1690 c, t dbSNP:758618906
1704 1704 c, t dbSNP:545096620
1740 1740 a, g dbSNP:185907899
1794 1794 c, t dbSNP:750538987
1892 1892 a, c dbSNP:151190415
2003 2003 a, g dbSNP:534031511
2048 2048 a, c dbSNP:566768714
2061 2061 c, g dbSNP:142139835
2097 2097 a, t dbSNP:181696565
2103 2103 -, a dbSNP:746048384
2126 2126 a, t dbSNP:534206104
2129 2129 c, t dbSNP:550623161
2156 2156 g, t dbSNP:556727493
2167 2167 a, g dbSNP:11940642
2309 2309 c, t dbSNP:148636320
2363 2363 -, tttc dbSNP:530078817
2414 2414 a, t dbSNP:767509484
2426 2426 a, g dbSNP:545729393
2428 2428 -, ta dbSNP:774144170
2441 2441 a, t dbSNP:546184664
2505 2505 a, g dbSNP:528798972
2552 2552 a, g dbSNP:561229523
2649 2649 c, g dbSNP:549337295
2692 2692 a, g dbSNP:531103173
2735 2735 c, t dbSNP:546846597
2772 2772 a, c dbSNP:532156248
2781 2781 a, g dbSNP:189901544
2795 2795 a, c dbSNP:767418097
2815 2815 c, t dbSNP:545446816
2906 2906 a, g dbSNP:528393181
2914 2914 a, t dbSNP:146441639
2928 2928 g, t dbSNP:76614198
2968 2968 a, g dbSNP:377013243
2999 2999 g, t dbSNP:543269935
3036 3036 a, g dbSNP:754926849
3071 3071 a, g dbSNP:770554417
3149 3149 a, t dbSNP:74590222
3163 3163 a, g dbSNP:541428629
3199 3199 c, t dbSNP:113242138
3200 3200 a, g dbSNP:554828236
3237 3237 c, t dbSNP:536079775
3238 3238 a, g dbSNP:139066500
3242 3242 a, c dbSNP:374830589
3254 3254 a, c dbSNP:146398570
3259 3259 a, g dbSNP:538771514
3277 3277 c, t dbSNP:574434155
3328 3328 a, g dbSNP:149424676
3367 3367 a, g dbSNP:370726249
3370 3370 a, g dbSNP:546222500
3428 3428 g, t dbSNP:79783621
3463 3463 a, g dbSNP:566860446
3464 3464 c, t dbSNP:549615698
3488 3488 a, t dbSNP:186704095
3518 3518 a, g dbSNP:747618421
3574 3574 a, g dbSNP:781681702
3603 3603 c, g dbSNP:547746685
3606 3606 g, t dbSNP:755457050
3621 3621 a, g dbSNP:751076278
3640 3640 a, c dbSNP:181240021
3655 3655 c, t dbSNP:780466040
3662 3662 c, t dbSNP:533507894
3718 3718 a, c dbSNP:559872777
3733 3733 c, g dbSNP:188459520
3738 3738 c, t dbSNP:139930263
3757 3757 a, g dbSNP:758609347
3766 3766 a, g dbSNP:561042544
3780 3780 a, g dbSNP:750676275
3788 3788 g, t dbSNP:183601926
3800 3800 a, g dbSNP:575446062
3832 3832 -, acttaa dbSNP:147020536
3834 3834 -, ttaac dbSNP:200765897
3852 3852 c, t dbSNP:60807717
3861 3861 g, t dbSNP:757432661
3943 3943 a, g dbSNP:753944036
3956 3956 a, g dbSNP:181941949
3957 3957 c, t dbSNP:767601206
4003 4003 c, g dbSNP:150664430
4020 4020 -, c dbSNP:143272176
4038 4038 c, t dbSNP:552620239
4039 4039 a, g dbSNP:11938181
4041 4041 a, c dbSNP:566884189
4064 4064 c, g dbSNP:141923297
4098 4098 c, t dbSNP:114689638
4099 4099 a, g dbSNP:72616968
4117 4117 a, t dbSNP:551985948
4237 4237 -, ctt dbSNP:762152762
4247 4247 c, t dbSNP:539367239
4292 4292 a, t dbSNP:138394198
4314 4314 c, g dbSNP:570383556
4323 4323 c, t dbSNP:189321020
4345 4345 c, t dbSNP:562157927
4347 4347 a, g dbSNP:543441899
4383 4383 a, g dbSNP:530866812
4411 4411 a, t dbSNP:563411388
4413 4413 a, g dbSNP:563416429
4416 4416 a, g dbSNP:766209739

Target ORF information:

RefSeq Version XM_005262893
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005262893

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAGA TTGGCGAGCG TGCTATTCTA
CTGGGAGGAC TCATCGTTGT ATGGGTTGGC TTCTTTATCT TGTTACCTTG GGGAAATCAA
TTTCCCAAAA TACAGTGGGA AGATTTGCAC AATAATTCAA TCCCTAATAC CACATTTGGG
GAAATTATTA TTGGTCTTTG GAAGTCTCCA ATGGAAGATG ACAATGAAAG ACCAACTGGT
TGCTCGATTG AACAAGCCTG GTGCCTCTAC ACCCCGGTGA TTCATCTGGC CCAGTTCCTT
ACATCAGCTG TGCTAATAGG ATTAGGCTAT CCAGTCTGCA ATCTTATGTC CTATACTCTA
TATTCAAAAA TTCTAGGACC AAAACCTCAG GGTGTATACA TGGGCTGGTT AACAGCATCT
GGAAGTGGAG CCCGGATTCT TGGGCCTATG TTCATCAGCC AAGTGTATGC TCACTGGGGA
CCACGATGGG CATTCAGCCT GGTGTGTGGA ATAATAGTGC TCACCATCAC CCTCCTGGGA
GTGGTTTACA AAAGACTCAT TGCTCTTTCT GTAAGATATG GGAGGATTCA GGAATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu78398D
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product major facilitator superfamily domain-containing protein 8 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGT TTTTCAGACT TGTTTTACAT TCCTTGGAGA AAAAGGTGTG
ACATGGGATG TGATTAAACT GCAGATAAAC ATGTATACAA CACCAGTTTT ACTTAGCGCC
TTCCTGGGAA TTTTAAATAT TATTCTGATC CTTGCCATAC TAAGAGAACA TCGTGTGGAT
GACTCAGGAA GACAGTGTAA AAGTATTAAT TTTGAAGAAG CAAGTACAGA TGAAGCTCAG
GTTCCCCAAG GAAATATTGA CCAGGTTGCT GTTGTGGCCA TCAATGTTCT GTTTTTTGTG
ACTCTATTTA TCTTTGCCCT TTTTGAAACC ATCATTACTC CATTAACAAT GGATATGTAT
GCCTGGACTC AAGAACAAGC TGTGTTATAT AATGGCATAA TACTTGCTGC TCTTGGGGTT
GAAGCCGTTG TTATTTTCTT AGGAGTTAAG TTGCTTTCCA AAAAGATTGG CGAGCGTGCT
ATTCTACTGG GAGGACTCAT CGTTGTATGG GTTGGCTTCT TTATCTTGTT ACCTTGGGGA
AATCAATTTC CCAAAATACA GTGGGAAGAT TTGCACAATA ATTCAATCCC TAATACCACA
TTTGGGGAAA TTATTATTGG TCTTTGGAAG TCTCCAATGG AAGATGACAA TGAAAGACCA
ACTGGTTGCT CGATTGAACA AGCCTGGTGC CTCTACACCC CGGTGATTCA TCTGGCCCAG
TTCCTTACAT CAGCTGTGCT AATAGGATTA GGCTATCCAG TCTGCAATCT TATGTCCTAT
ACTCTATATT CAAAAATTCT AGGACCAAAA CCTCAGGGTG TATACATGGG CTGGTTAACA
GCATCTGGAA GTGGAGCCCG GATTCTTGGG CCTATGTTCA TCAGCCAAGT GTATGCTCAC
TGGGGACCAC GATGGGCATT CAGCCTGGTG TGTGGAATAA TAGTGCTCAC CATCACCCTC
CTGGGAGTGG TTTACAAAAG ACTCATTGCT CTTTCTGTAA GATATGGGAG GATTCAGGAA
TAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011530132.1
CDS78..1520
Misc Feature(1)192..1472(+)
Misc Feature(2)201..>515(+)
Misc Feature(3)234..1358(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:532558333
23 23 c, g dbSNP:568845783
27 27 a, c dbSNP:760736619
35 35 a, g dbSNP:775119203
39 39 c, g dbSNP:374912082
41 41 a, t dbSNP:369032119
47 47 c, g dbSNP:771730847
50 50 a, c, t dbSNP:550627957
51 51 a, c, g, t dbSNP:375020549
56 56 a, g dbSNP:769126800
57 57 c, g, t dbSNP:200352565
58 58 a, g, t dbSNP:761604533
59 59 c, t dbSNP:750582324
60 60 c, t dbSNP:779186294
65 65 a, g dbSNP:757490861
67 67 a, g dbSNP:753641515
70 70 a, g dbSNP:763846149
71 71 c, t dbSNP:760642574
73 73 a, t dbSNP:752714009
77 77 c, t dbSNP:767069868
84 84 a, c, g dbSNP:773799172
91 91 c, g dbSNP:139409959
92 92 a, g dbSNP:762559863
93 93 a, c dbSNP:777004095
95 95 c, g dbSNP:771879274
99 99 a, g dbSNP:747446547
100 100 a, g dbSNP:780548594
102 102 g, t dbSNP:772629400
106 106 a, g dbSNP:532843679
108 108 a, g dbSNP:778902510
111 111 c, t dbSNP:371882083
112 112 c, t dbSNP:749496400
114 114 a, c dbSNP:150892838
117 117 g, t dbSNP:755977683
123 123 c, g dbSNP:752624076
128 128 a, c dbSNP:767558098
140 140 a, c dbSNP:201926015
143 143 a, t dbSNP:145529594
148 148 a, c dbSNP:761497836
149 149 c, t dbSNP:775812921
151 151 g, t dbSNP:767893733
154 154 g, t dbSNP:760049336
155 155 a, c, g dbSNP:770986122
179 179 c, t dbSNP:139212190
180 180 c, t dbSNP:749315686
181 181 a, g dbSNP:146596875
183 183 c, t dbSNP:200001979
192 192 a, g, t dbSNP:201739608
193 193 a, t dbSNP:140349255
196 196 c, g dbSNP:768336733
209 209 g, t dbSNP:746904557
230 230 a, g dbSNP:587781037
231 231 a, g dbSNP:779838200
233 233 a, g, t dbSNP:753433200
239 239 g, t dbSNP:777277246
248 248 a, g dbSNP:755862714
252 252 c, t dbSNP:751847064
254 254 c, t dbSNP:766788326
255 255 a, g dbSNP:143902749
256 256 c, t dbSNP:763512187
263 263 a, c dbSNP:750969105
268 268 c, t dbSNP:113960067
275 275 a, g dbSNP:765347608
276 276 a, g dbSNP:757876846
283 283 c, t dbSNP:147750747
284 284 a, g dbSNP:781051390
286 286 c, t dbSNP:570440254
293 293 c, t dbSNP:760772807
295 295 a, c dbSNP:377555060
296 296 a, g dbSNP:143654391
298 298 a, g dbSNP:759313071
303 303 c, t dbSNP:774200444
312 312 c, g, t dbSNP:749244700
333 333 a, g dbSNP:552923962
347 347 a, c, t dbSNP:754809138
354 354 a, g dbSNP:578102641
377 377 c, t dbSNP:754739634
383 383 a, g dbSNP:746201253
386 386 a, t dbSNP:148916499
391 391 a, g dbSNP:751274078
393 393 c, t dbSNP:757793193
396 396 c, t dbSNP:754403098
397 397 c, t dbSNP:764017632
403 403 g, t dbSNP:11732377
409 409 c, t dbSNP:145633957
419 419 c, t dbSNP:201905099
420 420 a, g dbSNP:183450731
427 427 c, t dbSNP:759795397
430 430 a, g dbSNP:774112195
435 435 c, g dbSNP:144845312
439 439 a, c, g dbSNP:118203978
449 449 c, t dbSNP:141031338
453 453 a, g dbSNP:773214617
459 459 c, g dbSNP:557910536
463 463 c, t dbSNP:769677706
466 466 a, g dbSNP:761377902
477 477 c, t dbSNP:776316095
480 480 a, g dbSNP:768398882
486 486 a, g dbSNP:146479250
488 488 -, tgctcgt dbSNP:765061912
488 488 c, t dbSNP:201652718
493 493 a, g, t dbSNP:749704755
498 498 c, t dbSNP:778330834
505 505 a, c, g dbSNP:762496121
512 512 a, c dbSNP:781299975
513 513 a, g dbSNP:755152088
518 518 g, t dbSNP:562103818
526 526 c, t dbSNP:766111411
536 536 a, g dbSNP:115275192
539 539 c, g dbSNP:777020801
551 551 a, g dbSNP:769124699
553 553 a, c, g, t dbSNP:28544073
553 553 -, g dbSNP:776253867
555 555 a, g dbSNP:377029630
562 562 g, t dbSNP:558274487
567 567 a, g dbSNP:757623473
584 584 a, g dbSNP:749064699
594 594 a, g dbSNP:777761765
597 597 a, g dbSNP:756069266
615 615 a, g dbSNP:368614789
617 617 c, t dbSNP:766992766
619 619 c, t dbSNP:112734134
621 621 c, g dbSNP:142063476
623 623 g, t dbSNP:765916112
640 640 c, t dbSNP:200591471
644 644 g, t dbSNP:754149229
647 647 c, g dbSNP:764470493
649 649 g, t dbSNP:761101343
652 652 c, t dbSNP:375681665
666 666 c, t dbSNP:779558035
669 669 c, t dbSNP:758002981
670 670 a, c, g dbSNP:371250204
682 682 c, g dbSNP:761017363
689 689 a, g dbSNP:753174925
692 692 g, t dbSNP:557451270
707 707 g, t dbSNP:199555198
730 730 a, c dbSNP:763119554
737 737 a, g dbSNP:773465316
750 750 a, g dbSNP:374234685
772 772 c, t dbSNP:200284184
774 774 a, g dbSNP:762117279
775 775 c, t dbSNP:776245759
776 776 c, t dbSNP:768499490
780 780 a, g dbSNP:144848697
781 781 a, g dbSNP:143288262
782 782 c, t dbSNP:772066492
783 783 a, g dbSNP:745354809
784 784 c, t dbSNP:778448039
789 789 g, t dbSNP:756815122
794 794 -, t dbSNP:775699005
801 801 c, g dbSNP:749001842
803 803 a, g dbSNP:199982542
809 809 a, c dbSNP:776272077
826 826 c, t dbSNP:755384900
837 837 c, t dbSNP:200631121
839 839 a, g dbSNP:770483205
844 844 a, c, t dbSNP:140948465
849 849 a, g dbSNP:147295085
857 857 c, g, t dbSNP:118203977
860 860 c, t dbSNP:747352043
867 867 c, g dbSNP:780583913
874 874 a, g dbSNP:371333586
876 876 a, g dbSNP:750969401
880 880 c, t dbSNP:200971312
886 886 a, g dbSNP:757345902
892 892 a, g dbSNP:118203975
894 894 a, g dbSNP:753930474
897 897 a, g dbSNP:77098161
898 898 c, t dbSNP:556875684
901 901 c, t dbSNP:752441316
907 907 a, c dbSNP:767331120
922 922 c, t dbSNP:759406697
923 923 c, t dbSNP:376954943
924 924 a, g dbSNP:76506918
926 926 a, t dbSNP:762491362
928 928 c, t dbSNP:772644224
938 938 a, g dbSNP:570989221
941 941 a, g dbSNP:138219028
968 968 a, c, t dbSNP:772735057
969 969 c, g dbSNP:150418024
970 970 a, c dbSNP:761499299
972 972 c, t dbSNP:373923336
973 973 a, g dbSNP:200386040
985 985 c, t dbSNP:768488730
998 998 c, t dbSNP:141422574
999 999 a, g dbSNP:746209973
1002 1002 c, g dbSNP:774629940
1004 1004 a, g dbSNP:148291156
1020 1020 a, g dbSNP:749791052
1024 1024 c, t dbSNP:777843146
1029 1029 a, c dbSNP:756204684
1031 1031 c, t dbSNP:376258320
1035 1035 a, g dbSNP:781391960
1037 1037 a, g dbSNP:755236045
1043 1043 a, g dbSNP:751316775
1065 1065 c, g dbSNP:727502800
1070 1070 c, g dbSNP:747197852
1078 1078 a, g dbSNP:779835846
1079 1079 a, t dbSNP:758114648
1094 1094 c, t dbSNP:760689362
1098 1098 a, t dbSNP:750297992
1099 1099 c, t dbSNP:191172038
1102 1102 a, g dbSNP:757306822
1104 1104 g, t dbSNP:724159970
1114 1114 c, t dbSNP:763730339
1116 1116 c, g dbSNP:11098943
1127 1127 g, t dbSNP:775018656
1131 1131 c, g dbSNP:767262517
1134 1134 a, t dbSNP:763226224
1137 1137 a, g dbSNP:773610115
1140 1140 a, g dbSNP:537799887
1141 1141 a, t dbSNP:762336516
1143 1143 a, g, t dbSNP:768627011
1155 1155 c, t dbSNP:747170947
1168 1168 c, t dbSNP:200745039
1169 1169 a, g dbSNP:772377066
1171 1171 c, t dbSNP:745633928
1176 1176 a, c dbSNP:778875017
1177 1177 a, g dbSNP:757222651
1181 1181 c, t dbSNP:753832653
1191 1191 c, t dbSNP:777361767
1194 1194 a, g dbSNP:755570201
1198 1198 c, t dbSNP:267607235
1199 1199 a, g dbSNP:767048437
1216 1216 a, g dbSNP:759285288
1218 1218 a, t dbSNP:748707366
1220 1220 c, t dbSNP:750743360
1225 1225 c, t dbSNP:765587961
1229 1229 a, g dbSNP:762246290
1231 1231 c, t dbSNP:3733319
1241 1241 a, g dbSNP:769272117
1243 1243 a, g dbSNP:760640742
1249 1249 a, g dbSNP:118203976
1250 1250 c, g, t dbSNP:138072045
1274 1274 c, g dbSNP:145453022
1279 1279 c, t dbSNP:778785216
1283 1283 a, g dbSNP:148864710
1285 1285 a, g dbSNP:749153763
1290 1290 a, g dbSNP:777570875
1291 1291 a, t dbSNP:756121904
1298 1298 a, g dbSNP:145518709
1308 1308 c, t dbSNP:371428597
1309 1309 c, t dbSNP:780779080
1320 1320 c, t dbSNP:769573477
1323 1323 a, g dbSNP:748118750
1324 1324 c, t dbSNP:559155109
1335 1335 a, g dbSNP:754467879
1340 1340 a, g dbSNP:185047041
1353 1353 a, g dbSNP:779648626
1354 1354 c, t dbSNP:758095101
1362 1362 a, c dbSNP:754100928
1371 1371 a, g dbSNP:764549054
1383 1383 c, g dbSNP:370663969
1386 1386 c, g dbSNP:528664888
1394 1394 g, t dbSNP:767966853
1407 1407 c, t dbSNP:724159971
1408 1408 a, c, g dbSNP:547726489
1421 1421 c, g dbSNP:766690694
1432 1432 a, g dbSNP:561194518
1438 1438 c, t dbSNP:183448311
1443 1443 c, t dbSNP:149683848
1457 1457 c, t dbSNP:769642042
1459 1459 -, tgggagtg dbSNP:756701118
1465 1465 c, t dbSNP:747948366
1471 1471 a, g dbSNP:780161755
1476 1476 a, c dbSNP:776622815
1479 1479 c, g dbSNP:570757797
1481 1481 c, t dbSNP:140732035
1484 1484 g, t dbSNP:779560159
1492 1492 c, t dbSNP:758005057
1497 1497 a, g dbSNP:745541140
1512 1512 c, g dbSNP:777943565
1521 1521 a, c, g dbSNP:753071746
1523 1523 c, t dbSNP:374487025
1543 1543 a, t dbSNP:766561103
1553 1553 a, g dbSNP:751643210
1558 1558 a, g dbSNP:766600653
1564 1564 a, c dbSNP:763024313
1566 1566 c, t dbSNP:750641784
1568 1568 g, t dbSNP:765439882
1576 1576 c, t dbSNP:758618906
1590 1590 c, t dbSNP:545096620
1626 1626 a, g dbSNP:185907899
1680 1680 c, t dbSNP:750538987
1778 1778 a, c dbSNP:151190415
1889 1889 a, g dbSNP:534031511
1934 1934 a, c dbSNP:566768714
1947 1947 c, g dbSNP:142139835
1983 1983 a, t dbSNP:181696565
1989 1989 -, a dbSNP:746048384
2012 2012 a, t dbSNP:534206104
2015 2015 c, t dbSNP:550623161
2042 2042 g, t dbSNP:556727493
2053 2053 a, g dbSNP:11940642
2195 2195 c, t dbSNP:148636320
2249 2249 -, tttc dbSNP:530078817
2300 2300 a, t dbSNP:767509484
2312 2312 a, g dbSNP:545729393
2314 2314 -, ta dbSNP:774144170
2327 2327 a, t dbSNP:546184664
2391 2391 a, g dbSNP:528798972
2438 2438 a, g dbSNP:561229523
2535 2535 c, g dbSNP:549337295
2578 2578 a, g dbSNP:531103173
2621 2621 c, t dbSNP:546846597
2658 2658 a, c dbSNP:532156248
2667 2667 a, g dbSNP:189901544
2681 2681 a, c dbSNP:767418097
2701 2701 c, t dbSNP:545446816
2792 2792 a, g dbSNP:528393181
2800 2800 a, t dbSNP:146441639
2814 2814 g, t dbSNP:76614198
2854 2854 a, g dbSNP:377013243
2885 2885 g, t dbSNP:543269935
2922 2922 a, g dbSNP:754926849
2957 2957 a, g dbSNP:770554417
3035 3035 a, t dbSNP:74590222
3049 3049 a, g dbSNP:541428629
3085 3085 c, t dbSNP:113242138
3086 3086 a, g dbSNP:554828236
3123 3123 c, t dbSNP:536079775
3124 3124 a, g dbSNP:139066500
3128 3128 a, c dbSNP:374830589
3140 3140 a, c dbSNP:146398570
3145 3145 a, g dbSNP:538771514
3163 3163 c, t dbSNP:574434155
3214 3214 a, g dbSNP:149424676
3253 3253 a, g dbSNP:370726249
3256 3256 a, g dbSNP:546222500
3314 3314 g, t dbSNP:79783621
3349 3349 a, g dbSNP:566860446
3350 3350 c, t dbSNP:549615698
3374 3374 a, t dbSNP:186704095
3404 3404 a, g dbSNP:747618421
3460 3460 a, g dbSNP:781681702
3489 3489 c, g dbSNP:547746685
3492 3492 g, t dbSNP:755457050
3507 3507 a, g dbSNP:751076278
3526 3526 a, c dbSNP:181240021
3541 3541 c, t dbSNP:780466040
3548 3548 c, t dbSNP:533507894
3604 3604 a, c dbSNP:559872777
3619 3619 c, g dbSNP:188459520
3624 3624 c, t dbSNP:139930263
3643 3643 a, g dbSNP:758609347
3652 3652 a, g dbSNP:561042544
3666 3666 a, g dbSNP:750676275
3674 3674 g, t dbSNP:183601926
3686 3686 a, g dbSNP:575446062
3718 3718 -, acttaa dbSNP:147020536
3720 3720 -, ttaac dbSNP:200765897
3738 3738 c, t dbSNP:60807717
3747 3747 g, t dbSNP:757432661
3829 3829 a, g dbSNP:753944036
3842 3842 a, g dbSNP:181941949
3843 3843 c, t dbSNP:767601206
3889 3889 c, g dbSNP:150664430
3906 3906 -, c dbSNP:143272176
3924 3924 c, t dbSNP:552620239
3925 3925 a, g dbSNP:11938181
3927 3927 a, c dbSNP:566884189
3950 3950 c, g dbSNP:141923297
3984 3984 c, t dbSNP:114689638
3985 3985 a, g dbSNP:72616968
4003 4003 a, t dbSNP:551985948
4123 4123 -, ctt dbSNP:762152762
4133 4133 c, t dbSNP:539367239
4178 4178 a, t dbSNP:138394198
4200 4200 c, g dbSNP:570383556
4209 4209 c, t dbSNP:189321020
4231 4231 c, t dbSNP:562157927
4233 4233 a, g dbSNP:543441899
4269 4269 a, g dbSNP:530866812
4297 4297 a, t dbSNP:563411388
4299 4299 a, g dbSNP:563416429
4302 4302 a, g dbSNP:766209739

Target ORF information:

RefSeq Version XM_011531830
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011531830

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGT TTTTCAGACT TGTTTTACAT TCCTTGGAGA AAAAGGTGTG
ACATGGGATG TGATTAAACT GCAGATAAAC ATGTATACAA CACCAGTTTT ACTTAGCGCC
TTCCTGGGAA TTTTAAATAT TATTCTGATC CTTGCCATAC TAAGAGAACA TCGTGTGGAT
GACTCAGGAA GACAGTGTAA AAGTATTAAT TTTGAAGAAG CAAGTACAGA TGAAGCTCAG
GTTCCCCAAG GAAATATTGA CCAGGTTGCT GTTGTGGCCA TCAATGTTCT GTTTTTTGTG
ACTCTATTTA TCTTTGCCCT TTTTGAAACC ATCATTACTC CATTAACAAT GGATATGTAT
GCCTGGACTC AAGAACAAGC TGTGTTATAT AATGGCATAA TACTTGCTGC TCTTGGGGTT
GAAGCCGTTG TTATTTTCTT AGGAGTTAAG TTGCTTTCCA AAAAGATTGG CGAGCGTGCT
ATTCTACTGG GAGGACTCAT CGTTGTATGG GTTGGCTTCT TTATCTTGTT ACCTTGGGGA
AATCAATTTC CCAAAATACA GTGGGAAGAT TTGCACAATA ATTCAATCCC TAATACCACA
TTTGGGGAAA TTATTATTGG TCTTTGGAAG TCTCCAATGG AAGATGACAA TGAAAGACCA
ACTGGTTGCT CGATTGAACA AGCCTGGTGC CTCTACACCC CGGTGATTCA TCTGGCCCAG
TTCCTTACAT CAGCTGTGCT AATAGGATTA GGCTATCCAG TCTGCAATCT TATGTCCTAT
ACTCTATATT CAAAAATTCT AGGACCAAAA CCTCAGGGTG TATACATGGG CTGGTTAACA
GCATCTGGAA GTGGAGCCCG GATTCTTGGG CCTATGTTCA TCAGCCAAGT GTATGCTCAC
TGGGGACCAC GATGGGCATT CAGCCTGGTG TGTGGAATAA TAGTGCTCAC CATCACCCTC
CTGGGAGTGG TTTACAAAAG ACTCATTGCT CTTTCTGTAA GATATGGGAG GATTCAGGAA
TAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu38845D
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product major facilitator superfamily domain-containing protein 8 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGAACATCG TGTGGATGAC TCAGGAAGAC AGTGTAAAAG TATTAATTTT
GAAGAAGCAA GTACAGATGA AGCTCAGGTT CCCCAAGGAA ATATTGACCA GGTTGCTGTT
GTGGCCATCA ATGTTCTGTT TTTTGTGACT CTATTTATCT TTGCCCTTTT TGAAACCATC
ATTACTCCAT TAACAATGGA TATGTATGCC TGGACTCAAG AACAAGCTGT GTTATATAAT
GGCATAATAC TTGCTGCTCT TGGGGTTGAA GCCGTTGTTA TTTTCTTAGG AGTTAAGTTG
CTTTCCAAAA AGATTGGCGA GCGTGCTATT CTACTGGGAG GACTCATCGT TGTATGGGTT
GGCTTCTTTA TCTTGTTACC TTGGGGAAAT CAATTTCCCA AAATACAGTG GGAAGATTTG
CACAATAATT CAATCCCTAA TACCACATTT GGGGAAATTA TTATTGGTCT TTGGAAGTCT
CCAATGGAAG ATGACAATGA AAGACCAACT GGTTGCTCGA TTGAACAAGC CTGGTGCCTC
TACACCCCGG TGATTCATCT GGCCCAGTTC CTTACATCAG CTGTGCTAAT AGGATTAGGC
TATCCAGTCT GCAATCTTAT GTCCTATACT CTATATTCAA AAATTCTAGG ACCAAAACCT
CAGGGTGTAT ACATGGGCTG GTTAACAGCA TCTGGAAGTG GAGCCCGGAT TCTTGGGCCT
ATGTTCATCA GCCAAGTGTA TGCTCACTGG GGACCACGAT GGGCATTCAG CCTGGTGTGT
GGAATAATAG TGCTCACCAT CACCCTCCTG GGAGTGGTTT ACAAAAGACT CATTGCTCTT
TCTGTAAGAT ATGGGAGGAT TCAGGAATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005262953.1
CDS78..1487
Misc Feature(1)192..1439(+)
Misc Feature(2)201..1337(+)
Misc Feature(3)234..1325(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:532558333
23 23 c, g dbSNP:568845783
27 27 a, c dbSNP:760736619
35 35 a, g dbSNP:775119203
39 39 c, g dbSNP:374912082
41 41 a, t dbSNP:369032119
47 47 c, g dbSNP:771730847
50 50 a, c, t dbSNP:550627957
51 51 a, c, g, t dbSNP:375020549
56 56 a, g dbSNP:769126800
57 57 c, g, t dbSNP:200352565
58 58 a, g, t dbSNP:761604533
59 59 c, t dbSNP:750582324
60 60 c, t dbSNP:779186294
65 65 a, g dbSNP:757490861
67 67 a, g dbSNP:753641515
70 70 a, g dbSNP:763846149
71 71 c, t dbSNP:760642574
73 73 a, t dbSNP:752714009
77 77 c, t dbSNP:767069868
84 84 a, c, g dbSNP:773799172
91 91 c, g dbSNP:139409959
92 92 a, g dbSNP:762559863
93 93 a, c dbSNP:777004095
95 95 c, g dbSNP:771879274
99 99 a, g dbSNP:747446547
100 100 a, g dbSNP:780548594
102 102 g, t dbSNP:772629400
106 106 a, g dbSNP:532843679
108 108 a, g dbSNP:778902510
111 111 c, t dbSNP:371882083
112 112 c, t dbSNP:749496400
114 114 a, c dbSNP:150892838
117 117 g, t dbSNP:755977683
123 123 c, g dbSNP:752624076
128 128 a, c dbSNP:767558098
140 140 a, c dbSNP:201926015
143 143 a, t dbSNP:145529594
148 148 a, c dbSNP:761497836
149 149 c, t dbSNP:775812921
151 151 g, t dbSNP:767893733
154 154 g, t dbSNP:760049336
155 155 a, c, g dbSNP:770986122
179 179 c, t dbSNP:139212190
180 180 c, t dbSNP:749315686
181 181 a, g dbSNP:146596875
183 183 c, t dbSNP:200001979
192 192 a, g, t dbSNP:201739608
193 193 a, t dbSNP:140349255
196 196 c, g dbSNP:768336733
209 209 g, t dbSNP:746904557
230 230 a, g dbSNP:587781037
231 231 a, g dbSNP:779838200
233 233 a, g, t dbSNP:753433200
239 239 g, t dbSNP:777277246
248 248 a, g dbSNP:755862714
252 252 c, t dbSNP:751847064
254 254 c, t dbSNP:766788326
255 255 a, g dbSNP:143902749
256 256 c, t dbSNP:763512187
263 263 a, c dbSNP:750969105
268 268 c, t dbSNP:113960067
275 275 a, g dbSNP:765347608
276 276 a, g dbSNP:757876846
283 283 c, t dbSNP:147750747
284 284 a, g dbSNP:781051390
286 286 c, t dbSNP:570440254
293 293 c, t dbSNP:760772807
295 295 a, c dbSNP:377555060
296 296 a, g dbSNP:143654391
298 298 a, g dbSNP:759313071
303 303 c, t dbSNP:774200444
312 312 c, g, t dbSNP:749244700
333 333 a, g dbSNP:552923962
347 347 a, c, t dbSNP:754809138
354 354 a, g dbSNP:578102641
377 377 c, t dbSNP:754739634
383 383 a, g dbSNP:746201253
386 386 a, t dbSNP:148916499
391 391 a, g dbSNP:751274078
393 393 c, t dbSNP:757793193
396 396 c, t dbSNP:754403098
397 397 c, t dbSNP:764017632
403 403 g, t dbSNP:11732377
409 409 c, t dbSNP:145633957
419 419 c, t dbSNP:201905099
420 420 a, g dbSNP:183450731
427 427 c, t dbSNP:759795397
430 430 a, g dbSNP:774112195
435 435 c, g dbSNP:144845312
439 439 a, c, g dbSNP:118203978
449 449 c, t dbSNP:141031338
453 453 a, g dbSNP:773214617
459 459 c, g dbSNP:557910536
463 463 c, t dbSNP:769677706
466 466 a, g dbSNP:761377902
477 477 c, t dbSNP:776316095
480 480 a, g dbSNP:768398882
486 486 a, g dbSNP:146479250
488 488 -, tgctcgt dbSNP:765061912
488 488 c, t dbSNP:201652718
493 493 a, g, t dbSNP:749704755
498 498 c, t dbSNP:778330834
505 505 a, c, g dbSNP:762496121
512 512 a, c dbSNP:781299975
513 513 a, g dbSNP:755152088
522 522 a, g dbSNP:780249947
525 525 a, g dbSNP:758584752
530 530 c, t dbSNP:750205976
542 542 c, t dbSNP:753346740
570 570 a, c dbSNP:761767969
575 575 a, g dbSNP:753834794
577 577 a, g dbSNP:763642606
581 581 c, t dbSNP:760252298
583 583 c, t dbSNP:775207180
591 591 a, g dbSNP:540305093
602 602 c, t dbSNP:556661896
608 608 a, g dbSNP:376733427
610 610 c, t dbSNP:201368623
618 618 a, t dbSNP:770057458
628 628 c, t dbSNP:201976338
633 633 c, t dbSNP:779558035
636 636 c, t dbSNP:758002981
637 637 a, c, g dbSNP:371250204
649 649 c, g dbSNP:761017363
656 656 a, g dbSNP:753174925
659 659 g, t dbSNP:557451270
674 674 g, t dbSNP:199555198
697 697 a, c dbSNP:763119554
704 704 a, g dbSNP:773465316
717 717 a, g dbSNP:374234685
739 739 c, t dbSNP:200284184
741 741 a, g dbSNP:762117279
742 742 c, t dbSNP:776245759
743 743 c, t dbSNP:768499490
747 747 a, g dbSNP:144848697
748 748 a, g dbSNP:143288262
749 749 c, t dbSNP:772066492
750 750 a, g dbSNP:745354809
751 751 c, t dbSNP:778448039
756 756 g, t dbSNP:756815122
761 761 -, t dbSNP:775699005
768 768 c, g dbSNP:749001842
770 770 a, g dbSNP:199982542
776 776 a, c dbSNP:776272077
793 793 c, t dbSNP:755384900
804 804 c, t dbSNP:200631121
806 806 a, g dbSNP:770483205
811 811 a, c, t dbSNP:140948465
816 816 a, g dbSNP:147295085
824 824 c, g, t dbSNP:118203977
827 827 c, t dbSNP:747352043
834 834 c, g dbSNP:780583913
841 841 a, g dbSNP:371333586
843 843 a, g dbSNP:750969401
847 847 c, t dbSNP:200971312
853 853 a, g dbSNP:757345902
859 859 a, g dbSNP:118203975
861 861 a, g dbSNP:753930474
864 864 a, g dbSNP:77098161
865 865 c, t dbSNP:556875684
868 868 c, t dbSNP:752441316
874 874 a, c dbSNP:767331120
889 889 c, t dbSNP:759406697
890 890 c, t dbSNP:376954943
891 891 a, g dbSNP:76506918
893 893 a, t dbSNP:762491362
895 895 c, t dbSNP:772644224
905 905 a, g dbSNP:570989221
908 908 a, g dbSNP:138219028
935 935 a, c, t dbSNP:772735057
936 936 c, g dbSNP:150418024
937 937 a, c dbSNP:761499299
939 939 c, t dbSNP:373923336
940 940 a, g dbSNP:200386040
952 952 c, t dbSNP:768488730
965 965 c, t dbSNP:141422574
966 966 a, g dbSNP:746209973
969 969 c, g dbSNP:774629940
971 971 a, g dbSNP:148291156
987 987 a, g dbSNP:749791052
991 991 c, t dbSNP:777843146
996 996 a, c dbSNP:756204684
998 998 c, t dbSNP:376258320
1002 1002 a, g dbSNP:781391960
1004 1004 a, g dbSNP:755236045
1010 1010 a, g dbSNP:751316775
1032 1032 c, g dbSNP:727502800
1037 1037 c, g dbSNP:747197852
1045 1045 a, g dbSNP:779835846
1046 1046 a, t dbSNP:758114648
1061 1061 c, t dbSNP:760689362
1065 1065 a, t dbSNP:750297992
1066 1066 c, t dbSNP:191172038
1069 1069 a, g dbSNP:757306822
1071 1071 g, t dbSNP:724159970
1081 1081 c, t dbSNP:763730339
1083 1083 c, g dbSNP:11098943
1094 1094 g, t dbSNP:775018656
1098 1098 c, g dbSNP:767262517
1101 1101 a, t dbSNP:763226224
1104 1104 a, g dbSNP:773610115
1107 1107 a, g dbSNP:537799887
1108 1108 a, t dbSNP:762336516
1110 1110 a, g, t dbSNP:768627011
1122 1122 c, t dbSNP:747170947
1135 1135 c, t dbSNP:200745039
1136 1136 a, g dbSNP:772377066
1138 1138 c, t dbSNP:745633928
1143 1143 a, c dbSNP:778875017
1144 1144 a, g dbSNP:757222651
1148 1148 c, t dbSNP:753832653
1158 1158 c, t dbSNP:777361767
1161 1161 a, g dbSNP:755570201
1165 1165 c, t dbSNP:267607235
1166 1166 a, g dbSNP:767048437
1183 1183 a, g dbSNP:759285288
1185 1185 a, t dbSNP:748707366
1187 1187 c, t dbSNP:750743360
1192 1192 c, t dbSNP:765587961
1196 1196 a, g dbSNP:762246290
1198 1198 c, t dbSNP:3733319
1208 1208 a, g dbSNP:769272117
1210 1210 a, g dbSNP:760640742
1216 1216 a, g dbSNP:118203976
1217 1217 c, g, t dbSNP:138072045
1241 1241 c, g dbSNP:145453022
1246 1246 c, t dbSNP:778785216
1250 1250 a, g dbSNP:148864710
1252 1252 a, g dbSNP:749153763
1257 1257 a, g dbSNP:777570875
1258 1258 a, t dbSNP:756121904
1265 1265 a, g dbSNP:145518709
1275 1275 c, t dbSNP:371428597
1276 1276 c, t dbSNP:780779080
1287 1287 c, t dbSNP:769573477
1290 1290 a, g dbSNP:748118750
1291 1291 c, t dbSNP:559155109
1302 1302 a, g dbSNP:754467879
1307 1307 a, g dbSNP:185047041
1320 1320 a, g dbSNP:779648626
1321 1321 c, t dbSNP:758095101
1329 1329 a, c dbSNP:754100928
1338 1338 a, g dbSNP:764549054
1350 1350 c, g dbSNP:370663969
1353 1353 c, g dbSNP:528664888
1361 1361 g, t dbSNP:767966853
1374 1374 c, t dbSNP:724159971
1375 1375 a, c, g dbSNP:547726489
1388 1388 c, g dbSNP:766690694
1399 1399 a, g dbSNP:561194518
1405 1405 c, t dbSNP:183448311
1410 1410 c, t dbSNP:149683848
1424 1424 c, t dbSNP:769642042
1426 1426 -, tgggagtg dbSNP:756701118
1432 1432 c, t dbSNP:747948366
1438 1438 a, g dbSNP:780161755
1443 1443 a, c dbSNP:776622815
1446 1446 c, g dbSNP:570757797
1448 1448 c, t dbSNP:140732035
1451 1451 g, t dbSNP:779560159
1459 1459 c, t dbSNP:758005057
1464 1464 a, g dbSNP:745541140
1479 1479 c, g dbSNP:777943565
1488 1488 a, c, g dbSNP:753071746
1490 1490 c, t dbSNP:374487025
1510 1510 a, t dbSNP:766561103
1520 1520 a, g dbSNP:751643210
1525 1525 a, g dbSNP:766600653
1531 1531 a, c dbSNP:763024313
1533 1533 c, t dbSNP:750641784
1535 1535 g, t dbSNP:765439882
1543 1543 c, t dbSNP:758618906
1557 1557 c, t dbSNP:545096620
1593 1593 a, g dbSNP:185907899
1647 1647 c, t dbSNP:750538987
1745 1745 a, c dbSNP:151190415
1856 1856 a, g dbSNP:534031511
1901 1901 a, c dbSNP:566768714
1914 1914 c, g dbSNP:142139835
1950 1950 a, t dbSNP:181696565
1956 1956 -, a dbSNP:746048384
1979 1979 a, t dbSNP:534206104
1982 1982 c, t dbSNP:550623161
2009 2009 g, t dbSNP:556727493
2020 2020 a, g dbSNP:11940642
2162 2162 c, t dbSNP:148636320
2216 2216 -, tttc dbSNP:530078817
2267 2267 a, t dbSNP:767509484
2279 2279 a, g dbSNP:545729393
2281 2281 -, ta dbSNP:774144170
2294 2294 a, t dbSNP:546184664
2358 2358 a, g dbSNP:528798972
2405 2405 a, g dbSNP:561229523
2502 2502 c, g dbSNP:549337295
2545 2545 a, g dbSNP:531103173
2588 2588 c, t dbSNP:546846597
2625 2625 a, c dbSNP:532156248
2634 2634 a, g dbSNP:189901544
2648 2648 a, c dbSNP:767418097
2668 2668 c, t dbSNP:545446816
2759 2759 a, g dbSNP:528393181
2767 2767 a, t dbSNP:146441639
2781 2781 g, t dbSNP:76614198
2821 2821 a, g dbSNP:377013243
2852 2852 g, t dbSNP:543269935
2889 2889 a, g dbSNP:754926849
2924 2924 a, g dbSNP:770554417
3002 3002 a, t dbSNP:74590222
3016 3016 a, g dbSNP:541428629
3052 3052 c, t dbSNP:113242138
3053 3053 a, g dbSNP:554828236
3090 3090 c, t dbSNP:536079775
3091 3091 a, g dbSNP:139066500
3095 3095 a, c dbSNP:374830589
3107 3107 a, c dbSNP:146398570
3112 3112 a, g dbSNP:538771514
3130 3130 c, t dbSNP:574434155
3181 3181 a, g dbSNP:149424676
3220 3220 a, g dbSNP:370726249
3223 3223 a, g dbSNP:546222500
3281 3281 g, t dbSNP:79783621
3316 3316 a, g dbSNP:566860446
3317 3317 c, t dbSNP:549615698
3341 3341 a, t dbSNP:186704095
3371 3371 a, g dbSNP:747618421
3427 3427 a, g dbSNP:781681702
3456 3456 c, g dbSNP:547746685
3459 3459 g, t dbSNP:755457050
3474 3474 a, g dbSNP:751076278
3493 3493 a, c dbSNP:181240021
3508 3508 c, t dbSNP:780466040
3515 3515 c, t dbSNP:533507894
3571 3571 a, c dbSNP:559872777
3586 3586 c, g dbSNP:188459520
3591 3591 c, t dbSNP:139930263
3610 3610 a, g dbSNP:758609347
3619 3619 a, g dbSNP:561042544
3633 3633 a, g dbSNP:750676275
3641 3641 g, t dbSNP:183601926
3653 3653 a, g dbSNP:575446062
3685 3685 -, acttaa dbSNP:147020536
3687 3687 -, ttaac dbSNP:200765897
3705 3705 c, t dbSNP:60807717
3714 3714 g, t dbSNP:757432661
3796 3796 a, g dbSNP:753944036
3809 3809 a, g dbSNP:181941949
3810 3810 c, t dbSNP:767601206
3856 3856 c, g dbSNP:150664430
3873 3873 -, c dbSNP:143272176
3891 3891 c, t dbSNP:552620239
3892 3892 a, g dbSNP:11938181
3894 3894 a, c dbSNP:566884189
3917 3917 c, g dbSNP:141923297
3951 3951 c, t dbSNP:114689638
3952 3952 a, g dbSNP:72616968
3970 3970 a, t dbSNP:551985948
4090 4090 -, ctt dbSNP:762152762
4100 4100 c, t dbSNP:539367239
4145 4145 a, t dbSNP:138394198
4167 4167 c, g dbSNP:570383556
4176 4176 c, t dbSNP:189321020
4198 4198 c, t dbSNP:562157927
4200 4200 a, g dbSNP:543441899
4236 4236 a, g dbSNP:530866812
4264 4264 a, t dbSNP:563411388
4266 4266 a, g dbSNP:563416429
4269 4269 a, g dbSNP:766209739

Target ORF information:

RefSeq Version XM_005262896
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005262896

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGAACATCG TGTGGATGAC TCAGGAAGAC AGTGTAAAAG TATTAATTTT
GAAGAAGCAA GTACAGATGA AGCTCAGGTT CCCCAAGGAA ATATTGACCA GGTTGCTGTT
GTGGCCATCA ATGTTCTGTT TTTTGTGACT CTATTTATCT TTGCCCTTTT TGAAACCATC
ATTACTCCAT TAACAATGGA TATGTATGCC TGGACTCAAG AACAAGCTGT GTTATATAAT
GGCATAATAC TTGCTGCTCT TGGGGTTGAA GCCGTTGTTA TTTTCTTAGG AGTTAAGTTG
CTTTCCAAAA AGATTGGCGA GCGTGCTATT CTACTGGGAG GACTCATCGT TGTATGGGTT
GGCTTCTTTA TCTTGTTACC TTGGGGAAAT CAATTTCCCA AAATACAGTG GGAAGATTTG
CACAATAATT CAATCCCTAA TACCACATTT GGGGAAATTA TTATTGGTCT TTGGAAGTCT
CCAATGGAAG ATGACAATGA AAGACCAACT GGTTGCTCGA TTGAACAAGC CTGGTGCCTC
TACACCCCGG TGATTCATCT GGCCCAGTTC CTTACATCAG CTGTGCTAAT AGGATTAGGC
TATCCAGTCT GCAATCTTAT GTCCTATACT CTATATTCAA AAATTCTAGG ACCAAAACCT
CAGGGTGTAT ACATGGGCTG GTTAACAGCA TCTGGAAGTG GAGCCCGGAT TCTTGGGCCT
ATGTTCATCA GCCAAGTGTA TGCTCACTGG GGACCACGAT GGGCATTCAG CCTGGTGTGT
GGAATAATAG TGCTCACCAT CACCCTCCTG GGAGTGGTTT ACAAAAGACT CATTGCTCTT
TCTGTAAGAT ATGGGAGGAT TCAGGAATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu38846D
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product major facilitator superfamily domain-containing protein 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGCAAGTAC AGATGAAGCT CAGGTTCCCC AAGGAAATAT TGACCAGGTT
GCTGTTGTGG CCATCAATGT TCTGTTTTTT GTGACTCTAT TTATCTTTGC CCTTTTTGAA
ACCATCATTA CTCCATTAAC AATGGATATG TATGCCTGGA CTCAAGAACA AGCTGTGTTA
TATAATGGCA TAATACTTGC TGCTCTTGGG GTTGAAGCCG TTGTTATTTT CTTAGGAGTT
AAGTTGCTTT CCAAAAAGAT TGGCGAGCGT GCTATTCTAC TGGGAGGACT CATCGTTGTA
TGGGTTGGCT TCTTTATCTT GTTACCTTGG GGAAATCAAT TTCCCAAAAT ACAGTGGGAA
GATTTGCACA ATAATTCAAT CCCTAATACC ACATTTGGGG AAATTATTAT TGGTCTTTGG
AAGTCTCCAA TGGAAGATGA CAATGAAAGA CCAACTGGTT GCTCGATTGA ACAAGCCTGG
TGCCTCTACA CCCCGGTGAT TCATCTGGCC CAGTTCCTTA CATCAGCTGT GCTAATAGGA
TTAGGCTATC CAGTCTGCAA TCTTATGTCC TATACTCTAT ATTCAAAAAT TCTAGGACCA
AAACCTCAGG GTGTATACAT GGGCTGGTTA ACAGCATCTG GAAGTGGAGC CCGGATTCTT
GGGCCTATGT TCATCAGCCA AGTGTATGCT CACTGGGGAC CACGATGGGC ATTCAGCCTG
GTGTGTGGAA TAATAGTGCT CACCATCACC CTCCTGGGAG TGGTTTACAA AAGACTCATT
GCTCTTTCTG TAAGATATGG GAGGATTCAG GAATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005262954.1
CDS78..1433
Misc Feature(1)192..1385(+)
Misc Feature(2)201..1283(+)
Misc Feature(3)234..1271(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:532558333
23 23 c, g dbSNP:568845783
27 27 a, c dbSNP:760736619
35 35 a, g dbSNP:775119203
39 39 c, g dbSNP:374912082
41 41 a, t dbSNP:369032119
47 47 c, g dbSNP:771730847
50 50 a, c, t dbSNP:550627957
51 51 a, c, g, t dbSNP:375020549
56 56 a, g dbSNP:769126800
57 57 c, g, t dbSNP:200352565
58 58 a, g, t dbSNP:761604533
59 59 c, t dbSNP:750582324
60 60 c, t dbSNP:779186294
65 65 a, g dbSNP:757490861
67 67 a, g dbSNP:753641515
70 70 a, g dbSNP:763846149
71 71 c, t dbSNP:760642574
73 73 a, t dbSNP:752714009
77 77 c, t dbSNP:767069868
84 84 a, c, g dbSNP:773799172
91 91 c, g dbSNP:139409959
92 92 a, g dbSNP:762559863
93 93 a, c dbSNP:777004095
95 95 c, g dbSNP:771879274
99 99 a, g dbSNP:747446547
100 100 a, g dbSNP:780548594
102 102 g, t dbSNP:772629400
106 106 a, g dbSNP:532843679
108 108 a, g dbSNP:778902510
111 111 c, t dbSNP:371882083
112 112 c, t dbSNP:749496400
114 114 a, c dbSNP:150892838
117 117 g, t dbSNP:755977683
123 123 c, g dbSNP:752624076
128 128 a, c dbSNP:767558098
140 140 a, c dbSNP:201926015
143 143 a, t dbSNP:145529594
148 148 a, c dbSNP:761497836
149 149 c, t dbSNP:775812921
151 151 g, t dbSNP:767893733
154 154 g, t dbSNP:760049336
155 155 a, c, g dbSNP:770986122
179 179 c, t dbSNP:139212190
180 180 c, t dbSNP:749315686
181 181 a, g dbSNP:146596875
183 183 c, t dbSNP:200001979
192 192 a, g, t dbSNP:201739608
193 193 a, t dbSNP:140349255
196 196 c, g dbSNP:768336733
209 209 g, t dbSNP:746904557
230 230 a, g dbSNP:587781037
231 231 a, g dbSNP:779838200
233 233 a, g, t dbSNP:753433200
239 239 g, t dbSNP:777277246
248 248 a, g dbSNP:755862714
252 252 c, t dbSNP:751847064
254 254 c, t dbSNP:766788326
255 255 a, g dbSNP:143902749
256 256 c, t dbSNP:763512187
263 263 a, c dbSNP:750969105
268 268 c, t dbSNP:113960067
275 275 a, g dbSNP:765347608
276 276 a, g dbSNP:757876846
283 283 c, t dbSNP:147750747
284 284 a, g dbSNP:781051390
286 286 c, t dbSNP:570440254
293 293 c, t dbSNP:760772807
295 295 a, c dbSNP:377555060
296 296 a, g dbSNP:143654391
298 298 a, g dbSNP:759313071
303 303 c, t dbSNP:774200444
312 312 c, g, t dbSNP:749244700
333 333 a, g dbSNP:552923962
347 347 a, c, t dbSNP:754809138
354 354 a, g dbSNP:578102641
377 377 c, t dbSNP:754739634
383 383 a, g dbSNP:746201253
386 386 a, t dbSNP:148916499
391 391 a, g dbSNP:751274078
393 393 c, t dbSNP:757793193
396 396 c, t dbSNP:754403098
397 397 c, t dbSNP:764017632
403 403 g, t dbSNP:11732377
409 409 c, t dbSNP:145633957
419 419 c, t dbSNP:201905099
420 420 a, g dbSNP:183450731
427 427 c, t dbSNP:759795397
430 430 a, g dbSNP:774112195
435 435 c, g dbSNP:144845312
439 439 a, c, g dbSNP:118203978
449 449 c, t dbSNP:141031338
453 453 a, g dbSNP:773214617
459 459 c, g dbSNP:557910536
463 463 c, t dbSNP:769677706
466 466 a, g dbSNP:761377902
477 477 c, t dbSNP:776316095
480 480 a, g dbSNP:768398882
486 486 a, g dbSNP:146479250
488 488 -, tgctcgt dbSNP:765061912
488 488 c, t dbSNP:201652718
493 493 a, g, t dbSNP:749704755
498 498 c, t dbSNP:778330834
505 505 a, c, g dbSNP:762496121
512 512 a, c dbSNP:781299975
513 513 a, g dbSNP:755152088
522 522 a, g dbSNP:780249947
525 525 a, g dbSNP:758584752
530 530 c, t dbSNP:750205976
542 542 c, t dbSNP:753346740
570 570 a, c dbSNP:761767969
575 575 a, g dbSNP:753834794
577 577 a, g dbSNP:763642606
581 581 c, t dbSNP:760252298
583 583 c, t dbSNP:775207180
591 591 a, g dbSNP:540305093
602 602 c, t dbSNP:556661896
608 608 a, g dbSNP:376733427
610 610 c, t dbSNP:201368623
618 618 a, t dbSNP:770057458
628 628 c, t dbSNP:201976338
643 643 a, c dbSNP:763119554
650 650 a, g dbSNP:773465316
663 663 a, g dbSNP:374234685
685 685 c, t dbSNP:200284184
687 687 a, g dbSNP:762117279
688 688 c, t dbSNP:776245759
689 689 c, t dbSNP:768499490
693 693 a, g dbSNP:144848697
694 694 a, g dbSNP:143288262
695 695 c, t dbSNP:772066492
696 696 a, g dbSNP:745354809
697 697 c, t dbSNP:778448039
702 702 g, t dbSNP:756815122
707 707 -, t dbSNP:775699005
714 714 c, g dbSNP:749001842
716 716 a, g dbSNP:199982542
722 722 a, c dbSNP:776272077
739 739 c, t dbSNP:755384900
750 750 c, t dbSNP:200631121
752 752 a, g dbSNP:770483205
757 757 a, c, t dbSNP:140948465
762 762 a, g dbSNP:147295085
770 770 c, g, t dbSNP:118203977
773 773 c, t dbSNP:747352043
780 780 c, g dbSNP:780583913
787 787 a, g dbSNP:371333586
789 789 a, g dbSNP:750969401
793 793 c, t dbSNP:200971312
799 799 a, g dbSNP:757345902
805 805 a, g dbSNP:118203975
807 807 a, g dbSNP:753930474
810 810 a, g dbSNP:77098161
811 811 c, t dbSNP:556875684
814 814 c, t dbSNP:752441316
820 820 a, c dbSNP:767331120
835 835 c, t dbSNP:759406697
836 836 c, t dbSNP:376954943
837 837 a, g dbSNP:76506918
839 839 a, t dbSNP:762491362
841 841 c, t dbSNP:772644224
851 851 a, g dbSNP:570989221
854 854 a, g dbSNP:138219028
881 881 a, c, t dbSNP:772735057
882 882 c, g dbSNP:150418024
883 883 a, c dbSNP:761499299
885 885 c, t dbSNP:373923336
886 886 a, g dbSNP:200386040
898 898 c, t dbSNP:768488730
911 911 c, t dbSNP:141422574
912 912 a, g dbSNP:746209973
915 915 c, g dbSNP:774629940
917 917 a, g dbSNP:148291156
933 933 a, g dbSNP:749791052
937 937 c, t dbSNP:777843146
942 942 a, c dbSNP:756204684
944 944 c, t dbSNP:376258320
948 948 a, g dbSNP:781391960
950 950 a, g dbSNP:755236045
956 956 a, g dbSNP:751316775
978 978 c, g dbSNP:727502800
983 983 c, g dbSNP:747197852
991 991 a, g dbSNP:779835846
992 992 a, t dbSNP:758114648
1007 1007 c, t dbSNP:760689362
1011 1011 a, t dbSNP:750297992
1012 1012 c, t dbSNP:191172038
1015 1015 a, g dbSNP:757306822
1017 1017 g, t dbSNP:724159970
1027 1027 c, t dbSNP:763730339
1029 1029 c, g dbSNP:11098943
1040 1040 g, t dbSNP:775018656
1044 1044 c, g dbSNP:767262517
1047 1047 a, t dbSNP:763226224
1050 1050 a, g dbSNP:773610115
1053 1053 a, g dbSNP:537799887
1054 1054 a, t dbSNP:762336516
1056 1056 a, g, t dbSNP:768627011
1068 1068 c, t dbSNP:747170947
1081 1081 c, t dbSNP:200745039
1082 1082 a, g dbSNP:772377066
1084 1084 c, t dbSNP:745633928
1089 1089 a, c dbSNP:778875017
1090 1090 a, g dbSNP:757222651
1094 1094 c, t dbSNP:753832653
1104 1104 c, t dbSNP:777361767
1107 1107 a, g dbSNP:755570201
1111 1111 c, t dbSNP:267607235
1112 1112 a, g dbSNP:767048437
1129 1129 a, g dbSNP:759285288
1131 1131 a, t dbSNP:748707366
1133 1133 c, t dbSNP:750743360
1138 1138 c, t dbSNP:765587961
1142 1142 a, g dbSNP:762246290
1144 1144 c, t dbSNP:3733319
1154 1154 a, g dbSNP:769272117
1156 1156 a, g dbSNP:760640742
1162 1162 a, g dbSNP:118203976
1163 1163 c, g, t dbSNP:138072045
1187 1187 c, g dbSNP:145453022
1192 1192 c, t dbSNP:778785216
1196 1196 a, g dbSNP:148864710
1198 1198 a, g dbSNP:749153763
1203 1203 a, g dbSNP:777570875
1204 1204 a, t dbSNP:756121904
1211 1211 a, g dbSNP:145518709
1221 1221 c, t dbSNP:371428597
1222 1222 c, t dbSNP:780779080
1233 1233 c, t dbSNP:769573477
1236 1236 a, g dbSNP:748118750
1237 1237 c, t dbSNP:559155109
1248 1248 a, g dbSNP:754467879
1253 1253 a, g dbSNP:185047041
1266 1266 a, g dbSNP:779648626
1267 1267 c, t dbSNP:758095101
1275 1275 a, c dbSNP:754100928
1284 1284 a, g dbSNP:764549054
1296 1296 c, g dbSNP:370663969
1299 1299 c, g dbSNP:528664888
1307 1307 g, t dbSNP:767966853
1320 1320 c, t dbSNP:724159971
1321 1321 a, c, g dbSNP:547726489
1334 1334 c, g dbSNP:766690694
1345 1345 a, g dbSNP:561194518
1351 1351 c, t dbSNP:183448311
1356 1356 c, t dbSNP:149683848
1370 1370 c, t dbSNP:769642042
1372 1372 -, tgggagtg dbSNP:756701118
1378 1378 c, t dbSNP:747948366
1384 1384 a, g dbSNP:780161755
1389 1389 a, c dbSNP:776622815
1392 1392 c, g dbSNP:570757797
1394 1394 c, t dbSNP:140732035
1397 1397 g, t dbSNP:779560159
1405 1405 c, t dbSNP:758005057
1410 1410 a, g dbSNP:745541140
1425 1425 c, g dbSNP:777943565
1434 1434 a, c, g dbSNP:753071746
1436 1436 c, t dbSNP:374487025
1456 1456 a, t dbSNP:766561103
1466 1466 a, g dbSNP:751643210
1471 1471 a, g dbSNP:766600653
1477 1477 a, c dbSNP:763024313
1479 1479 c, t dbSNP:750641784
1481 1481 g, t dbSNP:765439882
1489 1489 c, t dbSNP:758618906
1503 1503 c, t dbSNP:545096620
1539 1539 a, g dbSNP:185907899
1593 1593 c, t dbSNP:750538987
1691 1691 a, c dbSNP:151190415
1802 1802 a, g dbSNP:534031511
1847 1847 a, c dbSNP:566768714
1860 1860 c, g dbSNP:142139835
1896 1896 a, t dbSNP:181696565
1902 1902 -, a dbSNP:746048384
1925 1925 a, t dbSNP:534206104
1928 1928 c, t dbSNP:550623161
1955 1955 g, t dbSNP:556727493
1966 1966 a, g dbSNP:11940642
2108 2108 c, t dbSNP:148636320
2162 2162 -, tttc dbSNP:530078817
2213 2213 a, t dbSNP:767509484
2225 2225 a, g dbSNP:545729393
2227 2227 -, ta dbSNP:774144170
2240 2240 a, t dbSNP:546184664
2304 2304 a, g dbSNP:528798972
2351 2351 a, g dbSNP:561229523
2448 2448 c, g dbSNP:549337295
2491 2491 a, g dbSNP:531103173
2534 2534 c, t dbSNP:546846597
2571 2571 a, c dbSNP:532156248
2580 2580 a, g dbSNP:189901544
2594 2594 a, c dbSNP:767418097
2614 2614 c, t dbSNP:545446816
2705 2705 a, g dbSNP:528393181
2713 2713 a, t dbSNP:146441639
2727 2727 g, t dbSNP:76614198
2767 2767 a, g dbSNP:377013243
2798 2798 g, t dbSNP:543269935
2835 2835 a, g dbSNP:754926849
2870 2870 a, g dbSNP:770554417
2948 2948 a, t dbSNP:74590222
2962 2962 a, g dbSNP:541428629
2998 2998 c, t dbSNP:113242138
2999 2999 a, g dbSNP:554828236
3036 3036 c, t dbSNP:536079775
3037 3037 a, g dbSNP:139066500
3041 3041 a, c dbSNP:374830589
3053 3053 a, c dbSNP:146398570
3058 3058 a, g dbSNP:538771514
3076 3076 c, t dbSNP:574434155
3127 3127 a, g dbSNP:149424676
3166 3166 a, g dbSNP:370726249
3169 3169 a, g dbSNP:546222500
3227 3227 g, t dbSNP:79783621
3262 3262 a, g dbSNP:566860446
3263 3263 c, t dbSNP:549615698
3287 3287 a, t dbSNP:186704095
3317 3317 a, g dbSNP:747618421
3373 3373 a, g dbSNP:781681702
3402 3402 c, g dbSNP:547746685
3405 3405 g, t dbSNP:755457050
3420 3420 a, g dbSNP:751076278
3439 3439 a, c dbSNP:181240021
3454 3454 c, t dbSNP:780466040
3461 3461 c, t dbSNP:533507894
3517 3517 a, c dbSNP:559872777
3532 3532 c, g dbSNP:188459520
3537 3537 c, t dbSNP:139930263
3556 3556 a, g dbSNP:758609347
3565 3565 a, g dbSNP:561042544
3579 3579 a, g dbSNP:750676275
3587 3587 g, t dbSNP:183601926
3599 3599 a, g dbSNP:575446062
3631 3631 -, acttaa dbSNP:147020536
3633 3633 -, ttaac dbSNP:200765897
3651 3651 c, t dbSNP:60807717
3660 3660 g, t dbSNP:757432661
3742 3742 a, g dbSNP:753944036
3755 3755 a, g dbSNP:181941949
3756 3756 c, t dbSNP:767601206
3802 3802 c, g dbSNP:150664430
3819 3819 -, c dbSNP:143272176
3837 3837 c, t dbSNP:552620239
3838 3838 a, g dbSNP:11938181
3840 3840 a, c dbSNP:566884189
3863 3863 c, g dbSNP:141923297
3897 3897 c, t dbSNP:114689638
3898 3898 a, g dbSNP:72616968
3916 3916 a, t dbSNP:551985948
4036 4036 -, ctt dbSNP:762152762
4046 4046 c, t dbSNP:539367239
4091 4091 a, t dbSNP:138394198
4113 4113 c, g dbSNP:570383556
4122 4122 c, t dbSNP:189321020
4144 4144 c, t dbSNP:562157927
4146 4146 a, g dbSNP:543441899
4182 4182 a, g dbSNP:530866812
4210 4210 a, t dbSNP:563411388
4212 4212 a, g dbSNP:563416429
4215 4215 a, g dbSNP:766209739

Target ORF information:

RefSeq Version XM_005262897
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005262897

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGCAAGTAC AGATGAAGCT CAGGTTCCCC AAGGAAATAT TGACCAGGTT
GCTGTTGTGG CCATCAATGT TCTGTTTTTT GTGACTCTAT TTATCTTTGC CCTTTTTGAA
ACCATCATTA CTCCATTAAC AATGGATATG TATGCCTGGA CTCAAGAACA AGCTGTGTTA
TATAATGGCA TAATACTTGC TGCTCTTGGG GTTGAAGCCG TTGTTATTTT CTTAGGAGTT
AAGTTGCTTT CCAAAAAGAT TGGCGAGCGT GCTATTCTAC TGGGAGGACT CATCGTTGTA
TGGGTTGGCT TCTTTATCTT GTTACCTTGG GGAAATCAAT TTCCCAAAAT ACAGTGGGAA
GATTTGCACA ATAATTCAAT CCCTAATACC ACATTTGGGG AAATTATTAT TGGTCTTTGG
AAGTCTCCAA TGGAAGATGA CAATGAAAGA CCAACTGGTT GCTCGATTGA ACAAGCCTGG
TGCCTCTACA CCCCGGTGAT TCATCTGGCC CAGTTCCTTA CATCAGCTGT GCTAATAGGA
TTAGGCTATC CAGTCTGCAA TCTTATGTCC TATACTCTAT ATTCAAAAAT TCTAGGACCA
AAACCTCAGG GTGTATACAT GGGCTGGTTA ACAGCATCTG GAAGTGGAGC CCGGATTCTT
GGGCCTATGT TCATCAGCCA AGTGTATGCT CACTGGGGAC CACGATGGGC ATTCAGCCTG
GTGTGTGGAA TAATAGTGCT CACCATCACC CTCCTGGGAG TGGTTTACAA AAGACTCATT
GCTCTTTCTG TAAGATATGG GAGGATTCAG GAATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu78399D
Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product major facilitator superfamily domain-containing protein 8 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGC AAGTACAGAT GAAGCTCAGG TTCCCCAAGG AAATATTGAC
CAGGTTGCTG TTGTGGCCAT CAATGTTCTG TTTTTTGTGA CTCTATTTAT CTTTGCCCTT
TTTGAAACCA TCATTACTCC ATTAACAATG GATATGTATG CCTGGACTCA AGAACAAGCT
GTGTTATATA ATGGCATAAT ACTTGCTGCT CTTGGGGTTG AAGCCGTTGT TATTTTCTTA
GGAGTTAAGT TGCTTTCCAA AAAGATTGGC GAGCGTGCTA TTCTACTGGG AGGACTCATC
GTTGTATGGG TTGGCTTCTT TATCTTGTTA CCTTGGGGAA ATCAATTTCC CAAAATACAG
TGGGAAGATT TGCACAATAA TTCAATCCCT AATACCACAT TTGGGGAAAT TATTATTGGT
CTTTGGAAGT CTCCAATGGA AGATGACAAT GAAAGACCAA CTGGTTGCTC GATTGAACAA
GCCTGGTGCC TCTACACCCC GGTGATTCAT CTGGCCCAGT TCCTTACATC AGCTGTGCTA
ATAGGATTAG GCTATCCAGT CTGCAATCTT ATGTCCTATA CTCTATATTC AAAAATTCTA
GGACCAAAAC CTCAGGGTGT ATACATGGGC TGGTTAACAG CATCTGGAAG TGGAGCCCGG
ATTCTTGGGC CTATGTTCAT CAGCCAAGTG TATGCTCACT GGGGACCACG ATGGGCATTC
AGCCTGGTGT GTGGAATAAT AGTGCTCACC ATCACCCTCC TGGGAGTGGT TTACAAAAGA
CTCATTGCTC TTTCTGTAAG ATATGGGAGG ATTCAGGAATT AA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011530133.1
CDS78..1319
Misc Feature(1)192..1271(+)
Misc Feature(2)201..1169(+)
Misc Feature(3)234..1157(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:532558333
23 23 c, g dbSNP:568845783
27 27 a, c dbSNP:760736619
35 35 a, g dbSNP:775119203
39 39 c, g dbSNP:374912082
41 41 a, t dbSNP:369032119
47 47 c, g dbSNP:771730847
50 50 a, c, t dbSNP:550627957
51 51 a, c, g, t dbSNP:375020549
56 56 a, g dbSNP:769126800
57 57 c, g, t dbSNP:200352565
58 58 a, g, t dbSNP:761604533
59 59 c, t dbSNP:750582324
60 60 c, t dbSNP:779186294
65 65 a, g dbSNP:757490861
67 67 a, g dbSNP:753641515
70 70 a, g dbSNP:763846149
71 71 c, t dbSNP:760642574
73 73 a, t dbSNP:752714009
77 77 c, t dbSNP:767069868
84 84 a, c, g dbSNP:773799172
91 91 c, g dbSNP:139409959
92 92 a, g dbSNP:762559863
93 93 a, c dbSNP:777004095
95 95 c, g dbSNP:771879274
99 99 a, g dbSNP:747446547
100 100 a, g dbSNP:780548594
102 102 g, t dbSNP:772629400
106 106 a, g dbSNP:532843679
108 108 a, g dbSNP:778902510
111 111 c, t dbSNP:371882083
112 112 c, t dbSNP:749496400
114 114 a, c dbSNP:150892838
117 117 g, t dbSNP:755977683
123 123 c, g dbSNP:752624076
128 128 a, c dbSNP:767558098
140 140 a, c dbSNP:201926015
143 143 a, t dbSNP:145529594
148 148 a, c dbSNP:761497836
149 149 c, t dbSNP:775812921
151 151 g, t dbSNP:767893733
154 154 g, t dbSNP:760049336
155 155 a, c, g dbSNP:770986122
179 179 c, t dbSNP:139212190
180 180 c, t dbSNP:749315686
181 181 a, g dbSNP:146596875
183 183 c, t dbSNP:200001979
192 192 a, g, t dbSNP:201739608
193 193 a, t dbSNP:140349255
196 196 c, g dbSNP:768336733
209 209 g, t dbSNP:746904557
230 230 a, g dbSNP:587781037
231 231 a, g dbSNP:779838200
233 233 a, g, t dbSNP:753433200
239 239 g, t dbSNP:777277246
248 248 a, g dbSNP:755862714
252 252 c, t dbSNP:751847064
254 254 c, t dbSNP:766788326
255 255 a, g dbSNP:143902749
256 256 c, t dbSNP:763512187
263 263 a, c dbSNP:750969105
268 268 c, t dbSNP:113960067
275 275 a, g dbSNP:765347608
276 276 a, g dbSNP:757876846
283 283 c, t dbSNP:147750747
284 284 a, g dbSNP:781051390
286 286 c, t dbSNP:570440254
293 293 c, t dbSNP:760772807
295 295 a, c dbSNP:377555060
296 296 a, g dbSNP:143654391
298 298 a, g dbSNP:759313071
303 303 c, t dbSNP:774200444
312 312 c, g, t dbSNP:749244700
333 333 a, g dbSNP:552923962
347 347 a, c, t dbSNP:754809138
354 354 a, g dbSNP:578102641
377 377 c, t dbSNP:754739634
383 383 a, g dbSNP:746201253
386 386 a, t dbSNP:148916499
391 391 a, g dbSNP:751274078
393 393 c, t dbSNP:757793193
396 396 c, t dbSNP:754403098
397 397 c, t dbSNP:764017632
403 403 g, t dbSNP:11732377
409 409 c, t dbSNP:145633957
419 419 c, t dbSNP:201905099
420 420 a, g dbSNP:183450731
427 427 c, t dbSNP:759795397
430 430 a, g dbSNP:774112195
435 435 c, g dbSNP:144845312
439 439 a, c, g dbSNP:118203978
449 449 c, t dbSNP:141031338
453 453 a, g dbSNP:773214617
459 459 c, g dbSNP:557910536
463 463 c, t dbSNP:769677706
466 466 a, g dbSNP:761377902
477 477 c, t dbSNP:776316095
480 480 a, g dbSNP:768398882
486 486 a, g dbSNP:146479250
488 488 -, tgctcgt dbSNP:765061912
488 488 c, t dbSNP:201652718
493 493 a, g, t dbSNP:749704755
498 498 c, t dbSNP:778330834
505 505 a, c, g dbSNP:762496121
512 512 a, c dbSNP:781299975
513 513 a, g dbSNP:755152088
529 529 a, c dbSNP:763119554
536 536 a, g dbSNP:773465316
549 549 a, g dbSNP:374234685
571 571 c, t dbSNP:200284184
573 573 a, g dbSNP:762117279
574 574 c, t dbSNP:776245759
575 575 c, t dbSNP:768499490
579 579 a, g dbSNP:144848697
580 580 a, g dbSNP:143288262
581 581 c, t dbSNP:772066492
582 582 a, g dbSNP:745354809
583 583 c, t dbSNP:778448039
588 588 g, t dbSNP:756815122
593 593 -, t dbSNP:775699005
600 600 c, g dbSNP:749001842
602 602 a, g dbSNP:199982542
608 608 a, c dbSNP:776272077
625 625 c, t dbSNP:755384900
636 636 c, t dbSNP:200631121
638 638 a, g dbSNP:770483205
643 643 a, c, t dbSNP:140948465
648 648 a, g dbSNP:147295085
656 656 c, g, t dbSNP:118203977
659 659 c, t dbSNP:747352043
666 666 c, g dbSNP:780583913
673 673 a, g dbSNP:371333586
675 675 a, g dbSNP:750969401
679 679 c, t dbSNP:200971312
685 685 a, g dbSNP:757345902
691 691 a, g dbSNP:118203975
693 693 a, g dbSNP:753930474
696 696 a, g dbSNP:77098161
697 697 c, t dbSNP:556875684
700 700 c, t dbSNP:752441316
706 706 a, c dbSNP:767331120
721 721 c, t dbSNP:759406697
722 722 c, t dbSNP:376954943
723 723 a, g dbSNP:76506918
725 725 a, t dbSNP:762491362
727 727 c, t dbSNP:772644224
737 737 a, g dbSNP:570989221
740 740 a, g dbSNP:138219028
767 767 a, c, t dbSNP:772735057
768 768 c, g dbSNP:150418024
769 769 a, c dbSNP:761499299
771 771 c, t dbSNP:373923336
772 772 a, g dbSNP:200386040
784 784 c, t dbSNP:768488730
797 797 c, t dbSNP:141422574
798 798 a, g dbSNP:746209973
801 801 c, g dbSNP:774629940
803 803 a, g dbSNP:148291156
819 819 a, g dbSNP:749791052
823 823 c, t dbSNP:777843146
828 828 a, c dbSNP:756204684
830 830 c, t dbSNP:376258320
834 834 a, g dbSNP:781391960
836 836 a, g dbSNP:755236045
842 842 a, g dbSNP:751316775
864 864 c, g dbSNP:727502800
869 869 c, g dbSNP:747197852
877 877 a, g dbSNP:779835846
878 878 a, t dbSNP:758114648
893 893 c, t dbSNP:760689362
897 897 a, t dbSNP:750297992
898 898 c, t dbSNP:191172038
901 901 a, g dbSNP:757306822
903 903 g, t dbSNP:724159970
913 913 c, t dbSNP:763730339
915 915 c, g dbSNP:11098943
926 926 g, t dbSNP:775018656
930 930 c, g dbSNP:767262517
933 933 a, t dbSNP:763226224
936 936 a, g dbSNP:773610115
939 939 a, g dbSNP:537799887
940 940 a, t dbSNP:762336516
942 942 a, g, t dbSNP:768627011
954 954 c, t dbSNP:747170947
967 967 c, t dbSNP:200745039
968 968 a, g dbSNP:772377066
970 970 c, t dbSNP:745633928
975 975 a, c dbSNP:778875017
976 976 a, g dbSNP:757222651
980 980 c, t dbSNP:753832653
990 990 c, t dbSNP:777361767
993 993 a, g dbSNP:755570201
997 997 c, t dbSNP:267607235
998 998 a, g dbSNP:767048437
1015 1015 a, g dbSNP:759285288
1017 1017 a, t dbSNP:748707366
1019 1019 c, t dbSNP:750743360
1024 1024 c, t dbSNP:765587961
1028 1028 a, g dbSNP:762246290
1030 1030 c, t dbSNP:3733319
1040 1040 a, g dbSNP:769272117
1042 1042 a, g dbSNP:760640742
1048 1048 a, g dbSNP:118203976
1049 1049 c, g, t dbSNP:138072045
1073 1073 c, g dbSNP:145453022
1078 1078 c, t dbSNP:778785216
1082 1082 a, g dbSNP:148864710
1084 1084 a, g dbSNP:749153763
1089 1089 a, g dbSNP:777570875
1090 1090 a, t dbSNP:756121904
1097 1097 a, g dbSNP:145518709
1107 1107 c, t dbSNP:371428597
1108 1108 c, t dbSNP:780779080
1119 1119 c, t dbSNP:769573477
1122 1122 a, g dbSNP:748118750
1123 1123 c, t dbSNP:559155109
1134 1134 a, g dbSNP:754467879
1139 1139 a, g dbSNP:185047041
1152 1152 a, g dbSNP:779648626
1153 1153 c, t dbSNP:758095101
1161 1161 a, c dbSNP:754100928
1170 1170 a, g dbSNP:764549054
1182 1182 c, g dbSNP:370663969
1185 1185 c, g dbSNP:528664888
1193 1193 g, t dbSNP:767966853
1206 1206 c, t dbSNP:724159971
1207 1207 a, c, g dbSNP:547726489
1220 1220 c, g dbSNP:766690694
1231 1231 a, g dbSNP:561194518
1237 1237 c, t dbSNP:183448311
1242 1242 c, t dbSNP:149683848
1256 1256 c, t dbSNP:769642042
1258 1258 -, tgggagtg dbSNP:756701118
1264 1264 c, t dbSNP:747948366
1270 1270 a, g dbSNP:780161755
1275 1275 a, c dbSNP:776622815
1278 1278 c, g dbSNP:570757797
1280 1280 c, t dbSNP:140732035
1283 1283 g, t dbSNP:779560159
1291 1291 c, t dbSNP:758005057
1296 1296 a, g dbSNP:745541140
1311 1311 c, g dbSNP:777943565
1320 1320 a, c, g dbSNP:753071746
1322 1322 c, t dbSNP:374487025
1342 1342 a, t dbSNP:766561103
1352 1352 a, g dbSNP:751643210
1357 1357 a, g dbSNP:766600653
1363 1363 a, c dbSNP:763024313
1365 1365 c, t dbSNP:750641784
1367 1367 g, t dbSNP:765439882
1375 1375 c, t dbSNP:758618906
1389 1389 c, t dbSNP:545096620
1425 1425 a, g dbSNP:185907899
1479 1479 c, t dbSNP:750538987
1577 1577 a, c dbSNP:151190415
1688 1688 a, g dbSNP:534031511
1733 1733 a, c dbSNP:566768714
1746 1746 c, g dbSNP:142139835
1782 1782 a, t dbSNP:181696565
1788 1788 -, a dbSNP:746048384
1811 1811 a, t dbSNP:534206104
1814 1814 c, t dbSNP:550623161
1841 1841 g, t dbSNP:556727493
1852 1852 a, g dbSNP:11940642
1994 1994 c, t dbSNP:148636320
2048 2048 -, tttc dbSNP:530078817
2099 2099 a, t dbSNP:767509484
2111 2111 a, g dbSNP:545729393
2113 2113 -, ta dbSNP:774144170
2126 2126 a, t dbSNP:546184664
2190 2190 a, g dbSNP:528798972
2237 2237 a, g dbSNP:561229523
2334 2334 c, g dbSNP:549337295
2377 2377 a, g dbSNP:531103173
2420 2420 c, t dbSNP:546846597
2457 2457 a, c dbSNP:532156248
2466 2466 a, g dbSNP:189901544
2480 2480 a, c dbSNP:767418097
2500 2500 c, t dbSNP:545446816
2591 2591 a, g dbSNP:528393181
2599 2599 a, t dbSNP:146441639
2613 2613 g, t dbSNP:76614198
2653 2653 a, g dbSNP:377013243
2684 2684 g, t dbSNP:543269935
2721 2721 a, g dbSNP:754926849
2756 2756 a, g dbSNP:770554417
2834 2834 a, t dbSNP:74590222
2848 2848 a, g dbSNP:541428629
2884 2884 c, t dbSNP:113242138
2885 2885 a, g dbSNP:554828236
2922 2922 c, t dbSNP:536079775
2923 2923 a, g dbSNP:139066500
2927 2927 a, c dbSNP:374830589
2939 2939 a, c dbSNP:146398570
2944 2944 a, g dbSNP:538771514
2962 2962 c, t dbSNP:574434155
3013 3013 a, g dbSNP:149424676
3052 3052 a, g dbSNP:370726249
3055 3055 a, g dbSNP:546222500
3113 3113 g, t dbSNP:79783621
3148 3148 a, g dbSNP:566860446
3149 3149 c, t dbSNP:549615698
3173 3173 a, t dbSNP:186704095
3203 3203 a, g dbSNP:747618421
3259 3259 a, g dbSNP:781681702
3288 3288 c, g dbSNP:547746685
3291 3291 g, t dbSNP:755457050
3306 3306 a, g dbSNP:751076278
3325 3325 a, c dbSNP:181240021
3340 3340 c, t dbSNP:780466040
3347 3347 c, t dbSNP:533507894
3403 3403 a, c dbSNP:559872777
3418 3418 c, g dbSNP:188459520
3423 3423 c, t dbSNP:139930263
3442 3442 a, g dbSNP:758609347
3451 3451 a, g dbSNP:561042544
3465 3465 a, g dbSNP:750676275
3473 3473 g, t dbSNP:183601926
3485 3485 a, g dbSNP:575446062
3517 3517 -, acttaa dbSNP:147020536
3519 3519 -, ttaac dbSNP:200765897
3537 3537 c, t dbSNP:60807717
3546 3546 g, t dbSNP:757432661
3628 3628 a, g dbSNP:753944036
3641 3641 a, g dbSNP:181941949
3642 3642 c, t dbSNP:767601206
3688 3688 c, g dbSNP:150664430
3705 3705 -, c dbSNP:143272176
3723 3723 c, t dbSNP:552620239
3724 3724 a, g dbSNP:11938181
3726 3726 a, c dbSNP:566884189
3749 3749 c, g dbSNP:141923297
3783 3783 c, t dbSNP:114689638
3784 3784 a, g dbSNP:72616968
3802 3802 a, t dbSNP:551985948
3922 3922 -, ctt dbSNP:762152762
3932 3932 c, t dbSNP:539367239
3977 3977 a, t dbSNP:138394198
3999 3999 c, g dbSNP:570383556
4008 4008 c, t dbSNP:189321020
4030 4030 c, t dbSNP:562157927
4032 4032 a, g dbSNP:543441899
4068 4068 a, g dbSNP:530866812
4096 4096 a, t dbSNP:563411388
4098 4098 a, g dbSNP:563416429
4101 4101 a, g dbSNP:766209739

Target ORF information:

RefSeq Version XM_011531831
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens major facilitator superfamily domain containing 8 (MFSD8), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011531831

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGC AAGTACAGAT GAAGCTCAGG TTCCCCAAGG AAATATTGAC
CAGGTTGCTG TTGTGGCCAT CAATGTTCTG TTTTTTGTGA CTCTATTTAT CTTTGCCCTT
TTTGAAACCA TCATTACTCC ATTAACAATG GATATGTATG CCTGGACTCA AGAACAAGCT
GTGTTATATA ATGGCATAAT ACTTGCTGCT CTTGGGGTTG AAGCCGTTGT TATTTTCTTA
GGAGTTAAGT TGCTTTCCAA AAAGATTGGC GAGCGTGCTA TTCTACTGGG AGGACTCATC
GTTGTATGGG TTGGCTTCTT TATCTTGTTA CCTTGGGGAA ATCAATTTCC CAAAATACAG
TGGGAAGATT TGCACAATAA TTCAATCCCT AATACCACAT TTGGGGAAAT TATTATTGGT
CTTTGGAAGT CTCCAATGGA AGATGACAAT GAAAGACCAA CTGGTTGCTC GATTGAACAA
GCCTGGTGCC TCTACACCCC GGTGATTCAT CTGGCCCAGT TCCTTACATC AGCTGTGCTA
ATAGGATTAG GCTATCCAGT CTGCAATCTT ATGTCCTATA CTCTATATTC AAAAATTCTA
GGACCAAAAC CTCAGGGTGT ATACATGGGC TGGTTAACAG CATCTGGAAG TGGAGCCCGG
ATTCTTGGGC CTATGTTCAT CAGCCAAGTG TATGCTCACT GGGGACCACG ATGGGCATTC
AGCCTGGTGT GTGGAATAAT AGTGCTCACC ATCACCCTCC TGGGAGTGGT TTACAAAAGA
CTCATTGCTC TTTCTGTAAG ATATGGGAGG ATTCAGGAATT AA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu38847D
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product major facilitator superfamily domain-containing protein 8 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced gi:530377490. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCCGGCC TGCGGAACGA AAGTGAACAG GAGCCGCTCT TAGGCGACAC ACCTGGAAGC 
AGAGAATGGG ACATTTTAGA GACTGAAGAG CATTATAAGA GCCGATGGAG ATCTATTAGG
ATTTTATATC TTACTATGTT TCTCAGCAGT GTAGGGTTTT CTGTAGTGAT GATGTCCATA
TGGCCATATC TCCAAAAGAT TGATCCGACA GCTGATACAA GTTTTTTGGG CTGGGTTATT
GCTTCATATA GTCTTGGCCA AATGGTAGCT TCACCTATAT TTGGTTTATG GTCTAATTAT
AGACCAAGAA AAGAGCCTCT TATTGTCTCC ATCTTGATTT CCGTGGCAGC CAACTGCCTC
TATGCATATC TCCACATCCC AGCTTCTCAT AATAAATACT ACATGCTGGT TGCTCGTGGA
TTGTTGGGAA TTGGAGCAGG AAATGTAGCA GTTGTTAGAT CATATACTGC TGGTGCTACT
TCCCTTCAGG AAAGAACAAG TTCCATGGCA AACATAAGCA TGTGTCAAGC ATTAGGTTTT
ATTCTAGGTC CAGTTTTTCA GACTTGTTTT ACATTCCTTG GAGAAAAAGG TGTGACATGG
GATGTGATTA AACTGCAGAT AAACATGTAT ACAACACCAG TTTTACTTAG CGCCTTCCTG
GGAATTTTAA ATATTATTCT GATCCTTGCC ATACTAAGAG AACATCGTGT GGATGACTCA
GGAAGACAGT GTAAAAGTAT TAATTTTGAA GAAGCAAGTA CAGATGAAGC TCAGGTTCCC
CAAGGAAATA TTGACCAGGT TGCTGTTGTG GCCATCAATG TTCTGTTTTT TGTGACTCTA
TTTATCTTTG CCCTTTTTGA AACCATCATT ACTCCATTAA CAATGGATAT GTATGCCTGG
ACTCAAGAAC AAGCTGTGTT ATATAATGGC ATAATACTTG CTGCTCTTGG GGTTGAAGCC
GTTGTTATTT TCTTAGGAGT TAAGTTGCTT TCCAAAAAAT TTGCACAATTA A
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005262955.1
CDS78..1088
Misc Feature(1)192..>635(+)
Misc Feature(2)201..>635(+)
Translation
Position Chain Variation Link
18 18 a, g dbSNP:532558333
23 23 c, g dbSNP:568845783
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84 84 a, c, g dbSNP:773799172
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106 106 a, g dbSNP:532843679
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123 123 c, g dbSNP:752624076
128 128 a, c dbSNP:767558098
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148 148 a, c dbSNP:761497836
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181 181 a, g dbSNP:146596875
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209 209 g, t dbSNP:746904557
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263 263 a, c dbSNP:750969105
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275 275 a, g dbSNP:765347608
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403 403 g, t dbSNP:11732377
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488 488 -, tgctcgt dbSNP:765061912
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505 505 a, c, g dbSNP:762496121
512 512 a, c dbSNP:781299975
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522 522 a, g dbSNP:780249947
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542 542 c, t dbSNP:753346740
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618 618 a, t dbSNP:770057458
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640 640 c, t dbSNP:766111411
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1241