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NIPBL cDNA ORF clone, Homo sapiens (human)

Gene Symbol NIPBL
Entrez Gene ID 25836
Full Name Nipped-B homolog (Drosophila)
Synonyms CDLS, CDLS1, IDN3, IDN3-B, Scc2
General protein information
Preferred Names
nipped-B-like protein
Names
nipped-B-like protein
delangin
SCC2 homolog
sister chromatid cohesion 2 homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5p13.2

Summary This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

608667

Disorder Html: Cornelia de Lange syndrome 1, 122470 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_006714467 XP_006714530 nipped-B-like protein isoform X1
XM_006714468 XP_006714531 nipped-B-like protein isoform X2
XM_005248280 XP_005248337 nipped-B-like protein isoform X3
XM_011514014 XP_011512316 nipped-B-like protein isoform X4
XM_005248282 XP_005248339 nipped-B-like protein isoform X5
XM_011514015 XP_011512317 nipped-B-like protein isoform X6
NM_133433 NP_597677 nipped-B-like protein isoform A
NM_015384 NP_056199 nipped-B-like protein isoform B



Homo sapiens (human) NIPBL NP_597677.2
Pan troglodytes (chimpanzee) NIPBL XP_001146090.1
Macaca mulatta (Rhesus monkey) NIPBL XP_001096575.1
Canis lupus familiaris (dog) NIPBL XP_005619439.1
Bos taurus (cattle) NIPBL NP_001193513.1
Mus musculus (house mouse) Nipbl NP_081983.2
Rattus norvegicus (Norway rat) Nipbl XP_003749266.1
Gallus gallus (chicken) LOC427439 XP_425012.3
Gallus gallus (chicken) LOC427025 NP_001244277.1
Danio rerio (zebrafish) nipblb NP_001154919.2
Drosophila melanogaster (fruit fly) Nipped-B NP_001163052.1
Xenopus (Silurana) tropicalis (western clawed frog) nipbl XP_002938229.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu39306 XM_006714467 PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu39307 XM_006714468 PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu39308 XM_005248280 PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu66528 XM_011514014 PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu66529 XM_005248282 PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1301.30
$1859.00
OHu66530 XM_011514015 PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1301.30
$1859.00
OHu26171 NM_133433 Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu26399 NM_015384 Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu39306
Clone ID Related Accession (Same CDS sequence) XM_006714467
Accession Version XM_006714467.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8268bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578809900. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA
CGAAATTCAG ACTCTACGGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA CGTCATGGAT
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTGCAG
AAAACCAGCA GTGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT
GAGGCTAAGC GCCGTGATGG CCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT
CAGACTTTAC GATCCCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006714530.1
CDS489..8756
Misc Feature(1)3747..3971(+)
Misc Feature(2)5868..5993(+)
Misc Feature(3)7308..>7745(+)
Translation

Target ORF information:

RefSeq Version XM_006714467
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714467

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA
CGAAATTCAG ACTCTACGGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA CGTCATGGAT
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTGCAG
AAAACCAGCA GTGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT
GAGGCTAAGC GCCGTGATGG CCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT
CAGACTTTAC GATCCCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39307
Clone ID Related Accession (Same CDS sequence) XM_006714468
Accession Version XM_006714468.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8217bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATTTGCTA GGAAAATGAA GAAAAAAGAA AAACAGAAGA AAAGGAAAGC ATATGAACCA
AAACTAACAC CTGAAGAAAT GATGGACTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA
GAGAATATTT TGGATAATTT GGAAGATATG GATTTTACTG CGTTTGGTGA TGATGATGAA
ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT
AAAATAAAAG CAATGGGTAT AATGGATAAG CTTTCAACTG ACAAAACTGT GAAAGTCTTA
AATATCTTGG AGAAGAATAT TCAGGATGGG TCAAAGCTTT CCACTTTGTT AAATCATAAT
AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTTACAAAA
TCAGCGGATG CTTGTCTTAC AACTATCAAC ATTATGACAT CCCCTAACAT GCCAAAAGCT
GTGTACATTG AGGATGTAAT TGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT
ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTATTA
AGTTCAAAAG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT
TATAACAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAT TGCTAGAGAT ACAACTTCTT
ACAGACACAA CAATTCTTCA GGTTTCATCT ATGGGAATAA CACCATTTTT TGTGGAAAAT
GTCAGTGAAC TACAGTTGTG TGCCATTAAG TTAGTCACTG CAGTATTCTC AAGATATGAA
AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCAACCAGC
AAGAGGAGTT TAAGGAACTT CAGGTTAAAC AGTAGTGATA TGGATGGAGA ACCTATGTAT
ATTCAGATGG TTACAGCACT GGTTTTACAA CTTATTCAGT GTGTGGTACA CTTACCATCA
TCAGAGAAGG ACTCTAATGC AGAAGAAGAT TCAAATAAAA AAATTGACCA GGATGTTGTC
ATTACTAACT CTTATGAAAC AGCTATGCGA ACAGCCCAAA ACTTCCTCTC CATCTTCCTT
AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTGTTTGA AAATTTTGTT
CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT
TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAACAAGT CAACAGAGAT GGCTTTAAGA
GTGGCATCTC TTGATTACCT TGGAACTGTT GCTGCACGGC TAAGAAAAGA TGCTGTTACA
AGCAAAATGG ATCAAGGATC TATAGAACGC ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT
GAAATCCAAC AATTACAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC TGAGACTGAT
CCTTCACTAG TGTTTTCTCG TAAATTCTAT ATAGCCCAGT GGTTTCGAGA CACAACTCTG
GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AACACATCAT
GCAAAGGAAA TTGAGACAAC TGGCCAAATT ATGCATCGAG CTGAAAACCG AAAAAAGTTT
CTTAGAAGCA TTATCAAAAC CACACCTTCT CAGTTTAGCA CATTAAAGAT GAACTCTGAT
ACTGTGGACT ATGATGATGC TTGCTTGATT GTTCGATACT TGGCCTCCAT GAGGCCGTTT
GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTACGAG TTCTTGGTGA AAATGCAATT
GCTGTTCGAA CAAAAGCCAT GAAGTGTTTG TCTGAGGTTG TTGCTGTAGA CCCCAGTATT
CTAGCAAGGC TTGATATGCA ACGAGGTGTT CATGGACGAT TGATGGATAA TTCGACTAGT
GTCCGAGAAG CAGCAGTAGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT
GAACAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGGAAA
AGAGTAATAA AGATTCTCAG AGACATTTGT ATTGAACAAC CAACATTTCC AAAAATCACA
GAAATGTGTG TAAAAATGAT TCGCAGAGTC AATGATGAAG AGGGCATTAA GAAATTAGTA
AATGAAACAT TCCAGAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG
ACAAGGAAAA TTTTAAACAT TACCGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC
TGGTTTGAGC AACTGCTTCA AAACTTGTTG AAGTCCGAAG AGGATTCCTC ATATAAACCT
GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG
GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA
ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCAATGACT
ATGCAACCAT ACCTTACCAC TAAATGTAGT ACGCAAAATG ATTTCATGGT TATCTGCAAT
GTTGCAAAAA TCCTAGAGCT AGTTGTACCA CTGATGGAGC ATCCAAGTGA AACTTTTCTT
GCCACTATTG AGGAAGATCT AATGAAGCTC ATCATCAAAT ATGGCATGAC TGTAGTGCAA
CATTGTGTGA GCTGTCTTGG AGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG
TGGGCTTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG
GACCCAAATA ACACTTCACT TCTAACAAAC AAACCAGCAC TTCTTAGATC CCTTTTCACC
GTTGGAGCAC TATGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGCAAG
GTTAACATAA AAGATAAAGT ACTTGAACTA TTGATGTATT TTACAAAACA CTCAGATGAA
GAAGTACAAA CAAAAGCTAT CATTGGTCTA GGATTTGCCT TTATTCAGCA TCCAAGTCTA
ATGTTCGAGC AAGAAGTGAA GAATCTATAT AATAATATTT TATCTGATAA GAACTCCTCA
GTCAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA
CGTATGCAGC AGGCAGATAG AGACTGGAAG AAAGTTGCAA AACAGGAAGA CTTAAAAGAA
ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTATCT CAAACAGGTG
CTTGAGGCAT TTTTTCACAC CCAGTCAAGT GTACGCCACT TTGCCCTAAA TGTCATTGCA
TTGACTCTAA ATCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCATATTT AATTGCTATG
GGCACAGACC CAGAACCTGC TATGCGGAAC AAGGCTGATC AGCAACTTGT GGAAATAGAC
AAAAAATATG CTGGATTCAT TCATATGAAA GCAGTGGCTG GTATGAAGAT GTCTTACCAG
GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCAG ACAAGACGAG
TCCTCTAGCG CTTTGTGTTC ACACCTTTAC TCCATGATCC GTGGAAACCG CCAACACAGA
CGAGCCTTTC TTATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGACGTGACT
ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCG
TTGTTTATAA TGCATCATAT AGACATTACA CTCTCAGTTT CTGGTAGTAA CCTACTGCAG
TCATTCAAGG AGTCTATGGT AAAGGACAAA AGGAAAGAGA GAAAATCATC ACCTAGTAAG
GAAAATGAGT CAAGCGACAG TGAAGAAGAA GTTTCCAGGC CTCGGAAGTC ACGGAAACGT
GTAGATTCAG ATTCAGATTC AGATTCAGAA GACGATATAA ATTCAGTGAT GAAATGTTTG
CCAGAAAATT CAGCTCCTTT AATCGAATTT GCAAATGTGT CCCAGGGTAT TTTATTACTT
CTCATGTTAA AACAACATTT GAAGAATCTT TGTGGATTTT CTGATAGTAA AATTCAGAAG
TACTCTCCAT CTGAATCTGC AAAAGTATAT GATAAAGCGA TAAACCGAAA AACAGGAGTT
CATTTTCATC CAAAACAAAC ACTGGACTTC CTGCGGAGTG ACATGGCTAA TTCCAAAATC
ACAGAAGAGG TGAAAAGGAG TATAGTAAAA CAGTATCTAG ATTTCAAACT TCTCATGGAA
CATCTGGACC CTGATGAAGA AGAAGAAGAA GGGGAGGTTT CAGCTAGCAC AAATGCTCGG
AACAAAGCAA TTACCTCACT GCTTGGAGGA GGCAGCCCTA AAAATAATAC AGCAGCAGAG
ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA
AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC
GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ATCGACCACA AATTGCAAGA
GTAGTGCAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC
TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT
AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT
AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006714531.1
CDS489..8705
Misc Feature(1)5670..5795(+)
Misc Feature(2)7110..7661(+)
Translation

Target ORF information:

RefSeq Version XM_006714468
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714468

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATTTGCTA GGAAAATGAA GAAAAAAGAA AAACAGAAGA AAAGGAAAGC ATATGAACCA
AAACTAACAC CTGAAGAAAT GATGGACTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA
GAGAATATTT TGGATAATTT GGAAGATATG GATTTTACTG CGTTTGGTGA TGATGATGAA
ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT
AAAATAAAAG CAATGGGTAT AATGGATAAG CTTTCAACTG ACAAAACTGT GAAAGTCTTA
AATATCTTGG AGAAGAATAT TCAGGATGGG TCAAAGCTTT CCACTTTGTT AAATCATAAT
AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTTACAAAA
TCAGCGGATG CTTGTCTTAC AACTATCAAC ATTATGACAT CCCCTAACAT GCCAAAAGCT
GTGTACATTG AGGATGTAAT TGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT
ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTATTA
AGTTCAAAAG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT
TATAACAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAT TGCTAGAGAT ACAACTTCTT
ACAGACACAA CAATTCTTCA GGTTTCATCT ATGGGAATAA CACCATTTTT TGTGGAAAAT
GTCAGTGAAC TACAGTTGTG TGCCATTAAG TTAGTCACTG CAGTATTCTC AAGATATGAA
AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCAACCAGC
AAGAGGAGTT TAAGGAACTT CAGGTTAAAC AGTAGTGATA TGGATGGAGA ACCTATGTAT
ATTCAGATGG TTACAGCACT GGTTTTACAA CTTATTCAGT GTGTGGTACA CTTACCATCA
TCAGAGAAGG ACTCTAATGC AGAAGAAGAT TCAAATAAAA AAATTGACCA GGATGTTGTC
ATTACTAACT CTTATGAAAC AGCTATGCGA ACAGCCCAAA ACTTCCTCTC CATCTTCCTT
AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTGTTTGA AAATTTTGTT
CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT
TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAACAAGT CAACAGAGAT GGCTTTAAGA
GTGGCATCTC TTGATTACCT TGGAACTGTT GCTGCACGGC TAAGAAAAGA TGCTGTTACA
AGCAAAATGG ATCAAGGATC TATAGAACGC ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT
GAAATCCAAC AATTACAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC TGAGACTGAT
CCTTCACTAG TGTTTTCTCG TAAATTCTAT ATAGCCCAGT GGTTTCGAGA CACAACTCTG
GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AACACATCAT
GCAAAGGAAA TTGAGACAAC TGGCCAAATT ATGCATCGAG CTGAAAACCG AAAAAAGTTT
CTTAGAAGCA TTATCAAAAC CACACCTTCT CAGTTTAGCA CATTAAAGAT GAACTCTGAT
ACTGTGGACT ATGATGATGC TTGCTTGATT GTTCGATACT TGGCCTCCAT GAGGCCGTTT
GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTACGAG TTCTTGGTGA AAATGCAATT
GCTGTTCGAA CAAAAGCCAT GAAGTGTTTG TCTGAGGTTG TTGCTGTAGA CCCCAGTATT
CTAGCAAGGC TTGATATGCA ACGAGGTGTT CATGGACGAT TGATGGATAA TTCGACTAGT
GTCCGAGAAG CAGCAGTAGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT
GAACAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGGAAA
AGAGTAATAA AGATTCTCAG AGACATTTGT ATTGAACAAC CAACATTTCC AAAAATCACA
GAAATGTGTG TAAAAATGAT TCGCAGAGTC AATGATGAAG AGGGCATTAA GAAATTAGTA
AATGAAACAT TCCAGAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG
ACAAGGAAAA TTTTAAACAT TACCGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC
TGGTTTGAGC AACTGCTTCA AAACTTGTTG AAGTCCGAAG AGGATTCCTC ATATAAACCT
GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG
GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA
ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCAATGACT
ATGCAACCAT ACCTTACCAC TAAATGTAGT ACGCAAAATG ATTTCATGGT TATCTGCAAT
GTTGCAAAAA TCCTAGAGCT AGTTGTACCA CTGATGGAGC ATCCAAGTGA AACTTTTCTT
GCCACTATTG AGGAAGATCT AATGAAGCTC ATCATCAAAT ATGGCATGAC TGTAGTGCAA
CATTGTGTGA GCTGTCTTGG AGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG
TGGGCTTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG
GACCCAAATA ACACTTCACT TCTAACAAAC AAACCAGCAC TTCTTAGATC CCTTTTCACC
GTTGGAGCAC TATGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGCAAG
GTTAACATAA AAGATAAAGT ACTTGAACTA TTGATGTATT TTACAAAACA CTCAGATGAA
GAAGTACAAA CAAAAGCTAT CATTGGTCTA GGATTTGCCT TTATTCAGCA TCCAAGTCTA
ATGTTCGAGC AAGAAGTGAA GAATCTATAT AATAATATTT TATCTGATAA GAACTCCTCA
GTCAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA
CGTATGCAGC AGGCAGATAG AGACTGGAAG AAAGTTGCAA AACAGGAAGA CTTAAAAGAA
ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTATCT CAAACAGGTG
CTTGAGGCAT TTTTTCACAC CCAGTCAAGT GTACGCCACT TTGCCCTAAA TGTCATTGCA
TTGACTCTAA ATCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCATATTT AATTGCTATG
GGCACAGACC CAGAACCTGC TATGCGGAAC AAGGCTGATC AGCAACTTGT GGAAATAGAC
AAAAAATATG CTGGATTCAT TCATATGAAA GCAGTGGCTG GTATGAAGAT GTCTTACCAG
GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCAG ACAAGACGAG
TCCTCTAGCG CTTTGTGTTC ACACCTTTAC TCCATGATCC GTGGAAACCG CCAACACAGA
CGAGCCTTTC TTATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGACGTGACT
ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCG
TTGTTTATAA TGCATCATAT AGACATTACA CTCTCAGTTT CTGGTAGTAA CCTACTGCAG
TCATTCAAGG AGTCTATGGT AAAGGACAAA AGGAAAGAGA GAAAATCATC ACCTAGTAAG
GAAAATGAGT CAAGCGACAG TGAAGAAGAA GTTTCCAGGC CTCGGAAGTC ACGGAAACGT
GTAGATTCAG ATTCAGATTC AGATTCAGAA GACGATATAA ATTCAGTGAT GAAATGTTTG
CCAGAAAATT CAGCTCCTTT AATCGAATTT GCAAATGTGT CCCAGGGTAT TTTATTACTT
CTCATGTTAA AACAACATTT GAAGAATCTT TGTGGATTTT CTGATAGTAA AATTCAGAAG
TACTCTCCAT CTGAATCTGC AAAAGTATAT GATAAAGCGA TAAACCGAAA AACAGGAGTT
CATTTTCATC CAAAACAAAC ACTGGACTTC CTGCGGAGTG ACATGGCTAA TTCCAAAATC
ACAGAAGAGG TGAAAAGGAG TATAGTAAAA CAGTATCTAG ATTTCAAACT TCTCATGGAA
CATCTGGACC CTGATGAAGA AGAAGAAGAA GGGGAGGTTT CAGCTAGCAC AAATGCTCGG
AACAAAGCAA TTACCTCACT GCTTGGAGGA GGCAGCCCTA AAAATAATAC AGCAGCAGAG
ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA
AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC
GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ATCGACCACA AATTGCAAGA
GTAGTGCAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC
TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT
AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT
AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39308
Clone ID Related Accession (Same CDS sequence) XM_005248280
Accession Version XM_005248280.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530378759. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248337.1
CDS489..8585
Misc Feature(1)3747..3971(+)
Misc Feature(2)5868..5993(+)
Misc Feature(3)7308..7859(+)
Translation

Target ORF information:

RefSeq Version XM_005248280
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248280

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66528
Clone ID Related Accession (Same CDS sequence) XM_011514014
Accession Version XM_011514014.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GTTGCTAGGA AAATGAAGAA AAAAGAAAAA CAGAAGAAAA GGAAAGCATA TGAACCAAAA
CTAACACCTG AAGAAATGAT GGACTCTTCA ACTTTTAAGA GATTCACAGC CTCAATAGAG
AATATTTTGG ATAATTTGGA AGATATGGAT TTTACTGCGT TTGGTGATGA TGATGAAATT
CCTCAGGAAC TGCTCTTAGG AAAACATCAG CTTAATGAAC TTGGCAGTGA ATCTGCTAAA
ATAAAAGCAA TGGGTATAAT GGATAAGCTT TCAACTGACA AAACTGTGAA AGTCTTAAAT
ATCTTGGAGA AGAATATTCA GGATGGGTCA AAGCTTTCCA CTTTGTTAAA TCATAATAAC
GATACTGAAG AAGAAGAAAG GTTATGGAGA GACCTTATTA TGGAGAGAGT TACAAAATCA
GCGGATGCTT GTCTTACAAC TATCAACATT ATGACATCCC CTAACATGCC AAAAGCTGTG
TACATTGAGG ATGTAATTGA AAGAGTTATA CAGTACACTA AATTTCATTT GCAGAATACA
CTTTATCCTC AGTATGATCC TGTTTACAGA TTAGATCCTC ATGGAGGAGG CTTATTAAGT
TCAAAAGCAA AACGGGCTAA ATGTTCTACC CATAAGCAGA GAGTAATAGT AATGCTTTAT
AACAAAGTTT GTGACATTGT TAGCAGCTTA TCAGAATTGC TAGAGATACA ACTTCTTACA
GACACAACAA TTCTTCAGGT TTCATCTATG GGAATAACAC CATTTTTTGT GGAAAATGTC
AGTGAACTAC AGTTGTGTGC CATTAAGTTA GTCACTGCAG TATTCTCAAG ATATGAAAAA
CATAGGCAGT TAATTTTGGA AGAAATTTTT ACTTCACTTG CAAGATTACC AACCAGCAAG
AGGAGTTTAA GGAACTTCAG GTTAAACAGT AGTGATATGG ATGGAGAACC TATGTATATT
CAGATGGTTA CAGCACTGGT TTTACAACTT ATTCAGTGTG TGGTACACTT ACCATCATCA
GAGAAGGACT CTAATGCAGA AGAAGATTCA AATAAAAAAA TTGACCAGGA TGTTGTCATT
ACTAACTCTT ATGAAACAGC TATGCGAACA GCCCAAAACT TCCTCTCCAT CTTCCTTAAA
AAATGTGGTA GTAAGCAAGG TGAAGAAGAT TACAGACCAC TGTTTGAAAA TTTTGTTCAA
GACCTTCTTT CAACAGTCAA TAAGCCTGAA TGGCCAGCTG CTGAACTACT CCTTAGTTTG
TTAGGGAGAC TGTTGGTTCA TCAGTTCAGT AACAAGTCAA CAGAGATGGC TTTAAGAGTG
GCATCTCTTG ATTACCTTGG AACTGTTGCT GCACGGCTAA GAAAAGATGC TGTTACAAGC
AAAATGGATC AAGGATCTAT AGAACGCATT TTAAAACAGG TTTCAGGAGG GGAAGATGAA
ATCCAACAAT TACAAAAAGC ATTGCTTGAT TACTTGGATG AAAACACTGA GACTGATCCT
TCACTAGTGT TTTCTCGTAA ATTCTATATA GCCCAGTGGT TTCGAGACAC AACTCTGGAA
ACAGAAAAAG CAATGAAATC ACAAAAAGAT GAAGAATCAT CTGAAGGAAC ACATCATGCA
AAGGAAATTG AGACAACTGG CCAAATTATG CATCGAGCTG AAAACCGAAA AAAGTTTCTT
AGAAGCATTA TCAAAACCAC ACCTTCTCAG TTTAGCACAT TAAAGATGAA CTCTGATACT
GTGGACTATG ATGATGCTTG CTTGATTGTT CGATACTTGG CCTCCATGAG GCCGTTTGCC
CAGAGCTTTG ATATTTATTT GACACAGATC CTACGAGTTC TTGGTGAAAA TGCAATTGCT
GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGACCC CAGTATTCTA
GCAAGGCTTG ATATGCAACG AGGTGTTCAT GGACGATTGA TGGATAATTC GACTAGTGTC
CGAGAAGCAG CAGTAGAATT ACTAGGTCGA TTTGTCCTTT GTCGACCTCA GCTTGCTGAA
CAGTATTATG ATATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGGAAAAGA
GTAATAAAGA TTCTCAGAGA CATTTGTATT GAACAACCAA CATTTCCAAA AATCACAGAA
ATGTGTGTAA AAATGATTCG CAGAGTCAAT GATGAAGAGG GCATTAAGAA ATTAGTAAAT
GAAACATTCC AGAAACTCTG GTTTACTCCA ACTCCACACA ATGACAAAGA AGCAATGACA
AGGAAAATTT TAAACATTAC CGATGTGGTT GCAGCATGCA GAGATACTGG ATATGACTGG
TTTGAGCAAC TGCTTCAAAA CTTGTTGAAG TCCGAAGAGG ATTCCTCATA TAAACCTGTG
AAGAAAGCTT GTACTCAACT TGTTGATAAC CTAGTTGAGC ACATTCTTAA ATATGAGGAA
TCTCTAGCTG ACTCTGACAA TAAAGGTGTG AATTCTGGAA GATTGGTAGC TTGCATAACC
ACTTTGTTCT TATTCAGCAA AATAAGACCC CAGCTCATGG TTAAACATGC AATGACTATG
CAACCATACC TTACCACTAA ATGTAGTACG CAAAATGATT TCATGGTTAT CTGCAATGTT
GCAAAAATCC TAGAGCTAGT TGTACCACTG ATGGAGCATC CAAGTGAAAC TTTTCTTGCC
ACTATTGAGG AAGATCTAAT GAAGCTCATC ATCAAATATG GCATGACTGT AGTGCAACAT
TGTGTGAGCT GTCTTGGAGC TGTTGTAAAT AAAGTGACAC AAAATTTTAA ATTTGTGTGG
GCTTGTTTCA ATAGATACTA TGGTGCCATT TCAAAATTAA AAAGTCAACA CCAAGAGGAC
CCAAATAACA CTTCACTTCT AACAAACAAA CCAGCACTTC TTAGATCCCT TTTCACCGTT
GGAGCACTAT GTCGGCATTT TGATTTTGAT CTGGAAGATT TTAAAGGCAA CAGCAAGGTT
AACATAAAAG ATAAAGTACT TGAACTATTG ATGTATTTTA CAAAACACTC AGATGAAGAA
GTACAAACAA AAGCTATCAT TGGTCTAGGA TTTGCCTTTA TTCAGCATCC AAGTCTAATG
TTCGAGCAAG AAGTGAAGAA TCTATATAAT AATATTTTAT CTGATAAGAA CTCCTCAGTC
AATTTAAAAA TACAAGTGTT AAAAAACCTC CAGACCTACC TACAAGAAGA AGATACACGT
ATGCAGCAGG CAGATAGAGA CTGGAAGAAA GTTGCAAAAC AGGAAGACTT AAAAGAAATG
GGTGATGTTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TTTATCTCAA ACAGGTGCTT
GAGGCATTTT TTCACACCCA GTCAAGTGTA CGCCACTTTG CCCTAAATGT CATTGCATTG
ACTCTAAATC AAGGTCTTAT TCATCCAGTT CAGTGTGTGC CATATTTAAT TGCTATGGGC
ACAGACCCAG AACCTGCTAT GCGGAACAAG GCTGATCAGC AACTTGTGGA AATAGACAAA
AAATATGCTG GATTCATTCA TATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
CAACAGGCAA TCAACACATG CCTAAAAGAT CCTGTAAGGG GTTTCAGACA AGACGAGTCC
TCTAGCGCTT TGTGTTCACA CCTTTACTCC ATGATCCGTG GAAACCGCCA ACACAGACGA
GCCTTTCTTA TTTCTTTACT CAACCTCTTT GATGACACAG CAAAAACAGA CGTGACTATG
CTCTTGTATA TAGCAGACAA TCTAGCCTGT TTTCCATACC AGACACAGGA AGAGCCGTTG
TTTATAATGC ATCATATAGA CATTACACTC TCAGTTTCTG GTAGTAACCT ACTGCAGTCA
TTCAAGGAGT CTATGGTAAA GGACAAAAGG AAAGAGAGAA AATCATCACC TAGTAAGGAA
AATGAGTCAA GCGACAGTGA AGAAGAAGTT TCCAGGCCTC GGAAGTCACG GAAACGTGTA
GATTCAGATT CAGATTCAGA TTCAGAAGAC GATATAAATT CAGTGATGAA ATGTTTGCCA
GAAAATTCAG CTCCTTTAAT CGAATTTGCA AATGTGTCCC AGGGTATTTT ATTACTTCTC
ATGTTAAAAC AACATTTGAA GAATCTTTGT GGATTTTCTG ATAGTAAAAT TCAGAAGTAC
TCTCCATCTG AATCTGCAAA AGTATATGAT AAAGCGATAA ACCGAAAAAC AGGAGTTCAT
TTTCATCCAA AACAAACACT GGACTTCCTG CGGAGTGACA TGGCTAATTC CAAAATCACA
GAAGAGGTGA AAAGGAGTAT AGTAAAACAG TATCTAGATT TCAAACTTCT CATGGAACAT
CTGGACCCTG ATGAAGAAGA AGAAGAAGGG GAGGTTTCAG CTAGCACAAA TGCTCGGAAC
AAAGCAATTA CCTCACTGCT TGGAGGAGGC AGCCCTAAAA ATAATACAGC AGCAGAGACA
GAAGATGATG AAAGTGATGG GGAGGATAGA GGAGGAGGCA CTTCAGGGTC ATTGAGAAGG
TCAAAACGAA ATTCAGACTC TACGGAGTTG GCAGCACAGA TGAATGAAAG TGTTGACGTC
ATGGATGTCA TCGCTATTTG CTGTCCAAAG TACAAAGATC GACCACAAAT TGCAAGAGTA
GTGCAGAAAA CCAGCAGTGG CTTCAGTGTT CAGTGGATGG CAGGCTCCTA CAGTGGCTCC
TGGACTGAGG CTAAGCGCCG TGATGGCCGC AAACTGGTGC CTTGGGTAGA CACTATTAAA
GAGTCAGACA TTATTTACAA AAAAATTGCT CTAACGAGTG CTAATAAGCT GACTAATAAA
GTTGTTCAGA CTTTACGATC CCTGTATGCC GCCAAGGATG GGACTTCCAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512316.1
CDS489..8522
Misc Feature(1)5487..5612(+)
Misc Feature(2)6927..7478(+)
Translation

Target ORF information:

RefSeq Version XM_011514014
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514014

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GTTGCTAGGA AAATGAAGAA AAAAGAAAAA CAGAAGAAAA GGAAAGCATA TGAACCAAAA
CTAACACCTG AAGAAATGAT GGACTCTTCA ACTTTTAAGA GATTCACAGC CTCAATAGAG
AATATTTTGG ATAATTTGGA AGATATGGAT TTTACTGCGT TTGGTGATGA TGATGAAATT
CCTCAGGAAC TGCTCTTAGG AAAACATCAG CTTAATGAAC TTGGCAGTGA ATCTGCTAAA
ATAAAAGCAA TGGGTATAAT GGATAAGCTT TCAACTGACA AAACTGTGAA AGTCTTAAAT
ATCTTGGAGA AGAATATTCA GGATGGGTCA AAGCTTTCCA CTTTGTTAAA TCATAATAAC
GATACTGAAG AAGAAGAAAG GTTATGGAGA GACCTTATTA TGGAGAGAGT TACAAAATCA
GCGGATGCTT GTCTTACAAC TATCAACATT ATGACATCCC CTAACATGCC AAAAGCTGTG
TACATTGAGG ATGTAATTGA AAGAGTTATA CAGTACACTA AATTTCATTT GCAGAATACA
CTTTATCCTC AGTATGATCC TGTTTACAGA TTAGATCCTC ATGGAGGAGG CTTATTAAGT
TCAAAAGCAA AACGGGCTAA ATGTTCTACC CATAAGCAGA GAGTAATAGT AATGCTTTAT
AACAAAGTTT GTGACATTGT TAGCAGCTTA TCAGAATTGC TAGAGATACA ACTTCTTACA
GACACAACAA TTCTTCAGGT TTCATCTATG GGAATAACAC CATTTTTTGT GGAAAATGTC
AGTGAACTAC AGTTGTGTGC CATTAAGTTA GTCACTGCAG TATTCTCAAG ATATGAAAAA
CATAGGCAGT TAATTTTGGA AGAAATTTTT ACTTCACTTG CAAGATTACC AACCAGCAAG
AGGAGTTTAA GGAACTTCAG GTTAAACAGT AGTGATATGG ATGGAGAACC TATGTATATT
CAGATGGTTA CAGCACTGGT TTTACAACTT ATTCAGTGTG TGGTACACTT ACCATCATCA
GAGAAGGACT CTAATGCAGA AGAAGATTCA AATAAAAAAA TTGACCAGGA TGTTGTCATT
ACTAACTCTT ATGAAACAGC TATGCGAACA GCCCAAAACT TCCTCTCCAT CTTCCTTAAA
AAATGTGGTA GTAAGCAAGG TGAAGAAGAT TACAGACCAC TGTTTGAAAA TTTTGTTCAA
GACCTTCTTT CAACAGTCAA TAAGCCTGAA TGGCCAGCTG CTGAACTACT CCTTAGTTTG
TTAGGGAGAC TGTTGGTTCA TCAGTTCAGT AACAAGTCAA CAGAGATGGC TTTAAGAGTG
GCATCTCTTG ATTACCTTGG AACTGTTGCT GCACGGCTAA GAAAAGATGC TGTTACAAGC
AAAATGGATC AAGGATCTAT AGAACGCATT TTAAAACAGG TTTCAGGAGG GGAAGATGAA
ATCCAACAAT TACAAAAAGC ATTGCTTGAT TACTTGGATG AAAACACTGA GACTGATCCT
TCACTAGTGT TTTCTCGTAA ATTCTATATA GCCCAGTGGT TTCGAGACAC AACTCTGGAA
ACAGAAAAAG CAATGAAATC ACAAAAAGAT GAAGAATCAT CTGAAGGAAC ACATCATGCA
AAGGAAATTG AGACAACTGG CCAAATTATG CATCGAGCTG AAAACCGAAA AAAGTTTCTT
AGAAGCATTA TCAAAACCAC ACCTTCTCAG TTTAGCACAT TAAAGATGAA CTCTGATACT
GTGGACTATG ATGATGCTTG CTTGATTGTT CGATACTTGG CCTCCATGAG GCCGTTTGCC
CAGAGCTTTG ATATTTATTT GACACAGATC CTACGAGTTC TTGGTGAAAA TGCAATTGCT
GTTCGAACAA AAGCCATGAA GTGTTTGTCT GAGGTTGTTG CTGTAGACCC CAGTATTCTA
GCAAGGCTTG ATATGCAACG AGGTGTTCAT GGACGATTGA TGGATAATTC GACTAGTGTC
CGAGAAGCAG CAGTAGAATT ACTAGGTCGA TTTGTCCTTT GTCGACCTCA GCTTGCTGAA
CAGTATTATG ATATGCTGAT TGAAAGAATA TTGGATACTG GTATCAGTGT CAGGAAAAGA
GTAATAAAGA TTCTCAGAGA CATTTGTATT GAACAACCAA CATTTCCAAA AATCACAGAA
ATGTGTGTAA AAATGATTCG CAGAGTCAAT GATGAAGAGG GCATTAAGAA ATTAGTAAAT
GAAACATTCC AGAAACTCTG GTTTACTCCA ACTCCACACA ATGACAAAGA AGCAATGACA
AGGAAAATTT TAAACATTAC CGATGTGGTT GCAGCATGCA GAGATACTGG ATATGACTGG
TTTGAGCAAC TGCTTCAAAA CTTGTTGAAG TCCGAAGAGG ATTCCTCATA TAAACCTGTG
AAGAAAGCTT GTACTCAACT TGTTGATAAC CTAGTTGAGC ACATTCTTAA ATATGAGGAA
TCTCTAGCTG ACTCTGACAA TAAAGGTGTG AATTCTGGAA GATTGGTAGC TTGCATAACC
ACTTTGTTCT TATTCAGCAA AATAAGACCC CAGCTCATGG TTAAACATGC AATGACTATG
CAACCATACC TTACCACTAA ATGTAGTACG CAAAATGATT TCATGGTTAT CTGCAATGTT
GCAAAAATCC TAGAGCTAGT TGTACCACTG ATGGAGCATC CAAGTGAAAC TTTTCTTGCC
ACTATTGAGG AAGATCTAAT GAAGCTCATC ATCAAATATG GCATGACTGT AGTGCAACAT
TGTGTGAGCT GTCTTGGAGC TGTTGTAAAT AAAGTGACAC AAAATTTTAA ATTTGTGTGG
GCTTGTTTCA ATAGATACTA TGGTGCCATT TCAAAATTAA AAAGTCAACA CCAAGAGGAC
CCAAATAACA CTTCACTTCT AACAAACAAA CCAGCACTTC TTAGATCCCT TTTCACCGTT
GGAGCACTAT GTCGGCATTT TGATTTTGAT CTGGAAGATT TTAAAGGCAA CAGCAAGGTT
AACATAAAAG ATAAAGTACT TGAACTATTG ATGTATTTTA CAAAACACTC AGATGAAGAA
GTACAAACAA AAGCTATCAT TGGTCTAGGA TTTGCCTTTA TTCAGCATCC AAGTCTAATG
TTCGAGCAAG AAGTGAAGAA TCTATATAAT AATATTTTAT CTGATAAGAA CTCCTCAGTC
AATTTAAAAA TACAAGTGTT AAAAAACCTC CAGACCTACC TACAAGAAGA AGATACACGT
ATGCAGCAGG CAGATAGAGA CTGGAAGAAA GTTGCAAAAC AGGAAGACTT AAAAGAAATG
GGTGATGTTT CCTCAGGGAT GAGTAGTTCC ATCATGCAGC TTTATCTCAA ACAGGTGCTT
GAGGCATTTT TTCACACCCA GTCAAGTGTA CGCCACTTTG CCCTAAATGT CATTGCATTG
ACTCTAAATC AAGGTCTTAT TCATCCAGTT CAGTGTGTGC CATATTTAAT TGCTATGGGC
ACAGACCCAG AACCTGCTAT GCGGAACAAG GCTGATCAGC AACTTGTGGA AATAGACAAA
AAATATGCTG GATTCATTCA TATGAAAGCA GTGGCTGGTA TGAAGATGTC TTACCAGGTA
CAACAGGCAA TCAACACATG CCTAAAAGAT CCTGTAAGGG GTTTCAGACA AGACGAGTCC
TCTAGCGCTT TGTGTTCACA CCTTTACTCC ATGATCCGTG GAAACCGCCA ACACAGACGA
GCCTTTCTTA TTTCTTTACT CAACCTCTTT GATGACACAG CAAAAACAGA CGTGACTATG
CTCTTGTATA TAGCAGACAA TCTAGCCTGT TTTCCATACC AGACACAGGA AGAGCCGTTG
TTTATAATGC ATCATATAGA CATTACACTC TCAGTTTCTG GTAGTAACCT ACTGCAGTCA
TTCAAGGAGT CTATGGTAAA GGACAAAAGG AAAGAGAGAA AATCATCACC TAGTAAGGAA
AATGAGTCAA GCGACAGTGA AGAAGAAGTT TCCAGGCCTC GGAAGTCACG GAAACGTGTA
GATTCAGATT CAGATTCAGA TTCAGAAGAC GATATAAATT CAGTGATGAA ATGTTTGCCA
GAAAATTCAG CTCCTTTAAT CGAATTTGCA AATGTGTCCC AGGGTATTTT ATTACTTCTC
ATGTTAAAAC AACATTTGAA GAATCTTTGT GGATTTTCTG ATAGTAAAAT TCAGAAGTAC
TCTCCATCTG AATCTGCAAA AGTATATGAT AAAGCGATAA ACCGAAAAAC AGGAGTTCAT
TTTCATCCAA AACAAACACT GGACTTCCTG CGGAGTGACA TGGCTAATTC CAAAATCACA
GAAGAGGTGA AAAGGAGTAT AGTAAAACAG TATCTAGATT TCAAACTTCT CATGGAACAT
CTGGACCCTG ATGAAGAAGA AGAAGAAGGG GAGGTTTCAG CTAGCACAAA TGCTCGGAAC
AAAGCAATTA CCTCACTGCT TGGAGGAGGC AGCCCTAAAA ATAATACAGC AGCAGAGACA
GAAGATGATG AAAGTGATGG GGAGGATAGA GGAGGAGGCA CTTCAGGGTC ATTGAGAAGG
TCAAAACGAA ATTCAGACTC TACGGAGTTG GCAGCACAGA TGAATGAAAG TGTTGACGTC
ATGGATGTCA TCGCTATTTG CTGTCCAAAG TACAAAGATC GACCACAAAT TGCAAGAGTA
GTGCAGAAAA CCAGCAGTGG CTTCAGTGTT CAGTGGATGG CAGGCTCCTA CAGTGGCTCC
TGGACTGAGG CTAAGCGCCG TGATGGCCGC AAACTGGTGC CTTGGGTAGA CACTATTAAA
GAGTCAGACA TTATTTACAA AAAAATTGCT CTAACGAGTG CTAATAAGCT GACTAATAAA
GTTGTTCAGA CTTTACGATC CCTGTATGCC GCCAAGGATG GGACTTCCAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66529
Clone ID Related Accession (Same CDS sequence) XM_005248282
Accession Version XM_005248282.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7671bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578809904. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
ATGCTTTTGA ATTCCAACAC TTTACCTGAT GGAGATTCTT CAACAATGAG GAATGCTGCA 
TCTTTTCCCT TGAGATCTCC ACAGCCAGTA TGCTCCCCTG CTGGAAGTGA AGGAACTCCT
AAAGGCTCAA GACCACCTTT AATCCTACAA TCTCAGTCTC TACCTTGTTC ATCACCTCGA
GATGTTCCAC CAGATATCTT GCTAGATTCT CCAGAAAGAA AACAAAAGAA GCAGAAGAAA
ATGAAATTAG GCAAGGATGA AAAAGAGCAG AGTGAGAAAG CGGCAATGTA TGATATAATT
AGTTCTCCAT CCAAGGACTC TACTAAACTT ACATTAAGAC TTTCTCGTGT AAGGTCTTCA
GACATGGACC AGCAAGAGGA TATGATTTCT GGTGTGGAAA ATAGCAATGT TTCAGAAAAT
GATATTCCTT TTAATGTGCA GTACCCAGGA CAGACTTCAA AAACACCCAT TACTCCACAA
GATATAAACC GCCCACTAAA TGCTGCTCAA TGTTTGTCGC AGCAAGAACA AACAGCATTC
CTTCCAGCAA ATCAAGTGCC TGTTTTACAA CAGAACACTT CAGTTGCTGC AAAACAACCC
CAGACTTCTG TGGTACAGAA TCAACAACAG ATATCACAAC AGGGACCTAT ATATGATGAA
GTGGAATTGG ATGCATTGGC TGAAATTGAG CGAATAGAGA GAGAATCAGC TATTGAAAGG
GAGCGCTTCT CAAAAGAAGT TCAAGATAAA GATAAGCCTT TGAAAAAAAG AAAACAAGAT
TCTTACCCAC AGGAGGCTGG GGGTGCTACA GGAGGTAATA GACCAGCTTC TCAGGAGACG
GGTTCTACGG GAAATGGGTC AAGGCCAGCA TTAATGGTTA GCATTGATCT TCATCAGGCA
GGAAGAGTGG ACTCTCAGGC TTCTATAACT CAGGATTCAG ACTCCATAAA AAAGCCTGAA
GAAATCAAAC AATGTAATGA TGCACCTGTT TCTGTTCTTC AGGAAGATAT TGTTGGAAGT
CTTAAATCTA CACCAGAAAA CCATCCTGAG ACACCTAAAA AAAAGTCTGA TCCTGAGCTT
TCAAAGAGTG AAATGAAACA AAGTGAAAGT AGATTAGCAG AATCTAAACC AAATGAAAAC
CGATTGGTGG AGACAAAATC AAGTGAAAAT AAGTTAGAAA CTAAAGTTGA GACCCAAACA
GAAGAACTTA AACAGAATGA GAGCAGAACA ACTGAATGCA AACAAAACGA GAGCACCATA
GTTGAGCCTA AACAAAATGA AAATAGACTG TCTGACACAA AACCAAATGA CAACAAACAA
AATAATGGCA GATCAGAAAC AACAAAATCA AGGCCTGAAA CCCCAAAGCA AAAGGGTGAA
AGCCGGCCTG AGACTCCAAA ACAAAAGAGT GATGGGCATC CTGAAACCCC AAAACAGAAG
GGTGATGGAA GGCCTGAAAC TCCAAAGCAA AAAGGTGAGA GCCGCCCTGA AACTCCAAAG
CAAAAAAATG AAGGGCGACC TGAAACACCA AAACACAGGC ATGACAATAG GAGGGATTCT
GGAAAGCCAT CTACAGAGAA AAAACCTGAA GTGTCTAAAC ATAAACAAGA TACTAAATCT
GACTCACCTC GGTTAAAATC AGAACGAGCT GAAGCCTTAA AGCAGAGACC TGATGGGCGA
TCTGTTTCTG AGTCACTAAG ACGTGACCAT GATAATAAAC AAAAATCAGA TGACAGGGGT
GAATCAGAGC GACATCGAGG GGATCAGTCT AGGGTTCGAA GACCAGAAAC ATTGAGATCC
TCTAGTAGAA ATGAACATGG CATTAAATCT GATAGTTCAA AAACTGATAA ACTAGAACGA
AAACACAGGC ATGAATCAGG GGACTCAAGG GAAAGACCAT CTTCTGGGGA ACAAAAATCA
AGACCTGACA GTCCTCGTGT TAAACAAGGA GATTCTAATA AATCAAGATC TGATAAACTT
GGTTTTAAAT CACCAACTAG TAAAGATGAC AAAAGGACAG AGGGTAACAA GAGTAAAGTA
GACACTAATA AAGCACACCC TGACAATAAG GCAGAATTTC CAAGTTATTT GTTGGGGGGC
AGGTCTGGTG CGTTGAAAAA TTTTGTCATT CCGAAAATCA AGAGGGATAA AGATGGCAAT
GTTACTCAGG AGACAAAGAA AATGGAAATG AAAGGAGAGC CGAAAGACAA AGTAGAAAAA
ATAGGATTAG TTGAAGATCT AAATAAAGGA GCTAAGCCTG TAGTTGTGCT ACAAAAACTG
TCTTTGGATG ATGTTCAGAA ACTTATTAAA GATAGAGAGG ACAAATCAAG AAGTTCCCTT
AAACCTATCA AGAATAAACC ATCAAAGTCA AATAAAGGTA GTATAGATCA ATCAGTGTTA
AAAGAATTAC CCCCTGAACT CCTGGCAGAA ATTGAGTCCA CCATGCCACT TTGTGAACGT
GTGAAAATGA ACAAACGCAA GCGTAGCACA GTTAATGAAA AGCCAAAATA TGCTGAAATC
AGTTCAGATG AAGATAATGA TAGTGATGAA GCTTTTGAAT CCTCTAGGAA ACGACATAAA
AAAGATGATG ATAAAGCTTG GGAATATGAA GAGCGTGACA GAAGAAGCTC TGGGGATCAT
AGGAGAAGTG GCCACTCTCA TGAAGGAAGA AGGAGTTCAG GTGGTGGTCG TTATCGAAAC
CGAAGTCCGT CAGATTCTGA CATGGAAGAT TATTCTCCTC CTCCCAGCCT TAGTGAGGTT
GCTAGGAAAA TGAAGAAAAA AGAAAAACAG AAGAAAAGGA AAGCATATGA ACCAAAACTA
ACACCTGAAG AAATGATGGA CTCTTCAACT TTTAAGAGAT TCACAGCCTC AATAGAGAAT
ATTTTGGATA ATTTGGAAGA TATGGATTTT ACTGCGTTTG GTGATGATGA TGAAATTCCT
CAGGAACTGC TCTTAGGAAA ACATCAGCTT AATGAACTTG GCAGTGAATC TGCTAAAATA
AAAGCAATGG GTATAATGGA TAAGCTTTCA ACTGACAAAA CTGTGAAAGT CTTAAATATC
TTGGAGAAGA ATATTCAGGA TGGGTCAAAG CTTTCCACTT TGTTAAATCA TAATAACGAT
ACTGAAGAAG AAGAAAGGTT ATGGAGAGAC CTTATTATGG AGAGAGTTAC AAAATCAGCG
GATGCTTGTC TTACAACTAT CAACATTATG ACATCCCCTA ACATGCCAAA AGCTGTGTAC
ATTGAGGATG TAATTGAAAG AGTTATACAG TACACTAAAT TTCATTTGCA GAATACACTT
TATCCTCAGT ATGATCCTGT TTACAGATTA GATCCTCATG GAGGAGGCTT ATTAAGTTCA
AAAGCAAAAC GGGCTAAATG TTCTACCCAT AAGCAGAGAG TAATAGTAAT GCTTTATAAC
AAAGTTTGTG ACATTGTTAG CAGCTTATCA GAATTGCTAG AGATACAACT TCTTACAGAC
ACAACAATTC TTCAGGTTTC ATCTATGGGA ATAACACCAT TTTTTGTGGA AAATGTCAGT
GAACTACAGT TGTGTGCCAT TAAGTTAGTC ACTGCAGTAT TCTCAAGATA TGAAAAACAT
AGGCAGTTAA TTTTGGAAGA AATTTTTACT TCACTTGCAA GATTACCAAC CAGCAAGAGG
AGTTTAAGGA ACTTCAGGTT AAACAGTAGT GATATGGATG GAGAACCTAT GTATATTCAG
ATGGTTACAG CACTGGTTTT ACAACTTATT CAGTGTGTGG TACACTTACC ATCATCAGAG
AAGGACTCTA ATGCAGAAGA AGATTCAAAT AAAAAAATTG ACCAGGATGT TGTCATTACT
AACTCTTATG AAACAGCTAT GCGAACAGCC CAAAACTTCC TCTCCATCTT CCTTAAAAAA
TGTGGTAGTA AGCAAGGTGA AGAAGATTAC AGACCACTGT TTGAAAATTT TGTTCAAGAC
CTTCTTTCAA CAGTCAATAA GCCTGAATGG CCAGCTGCTG AACTACTCCT TAGTTTGTTA
GGGAGACTGT TGGTTCATCA GTTCAGTAAC AAGTCAACAG AGATGGCTTT AAGAGTGGCA
TCTCTTGATT ACCTTGGAAC TGTTGCTGCA CGGCTAAGAA AAGATGCTGT TACAAGCAAA
ATGGATCAAG GATCTATAGA ACGCATTTTA AAACAGGTTT CAGGAGGGGA AGATGAAATC
CAACAATTAC AAAAAGCATT GCTTGATTAC TTGGATGAAA ACACTGAGAC TGATCCTTCA
CTAGTGTTTT CTCGTAAATT CTATATAGCC CAGTGGTTTC GAGACACAAC TCTGGAAACA
GAAAAAGCAA TGAAATCACA AAAAGATGAA GAATCATCTG AAGGAACACA TCATGCAAAG
GAAATTGAGA CAACTGGCCA AATTATGCAT CGAGCTGAAA ACCGAAAAAA GTTTCTTAGA
AGCATTATCA AAACCACACC TTCTCAGTTT AGCACATTAA AGATGAACTC TGATACTGTG
GACTATGATG ATGCTTGCTT GATTGTTCGA TACTTGGCCT CCATGAGGCC GTTTGCCCAG
AGCTTTGATA TTTATTTGAC ACAGATCCTA CGAGTTCTTG GTGAAAATGC AATTGCTGTT
CGAACAAAAG CCATGAAGTG TTTGTCTGAG GTTGTTGCTG TAGACCCCAG TATTCTAGCA
AGGCTTGATA TGCAACGAGG TGTTCATGGA CGATTGATGG ATAATTCGAC TAGTGTCCGA
GAAGCAGCAG TAGAATTACT AGGTCGATTT GTCCTTTGTC GACCTCAGCT TGCTGAACAG
TATTATGATA TGCTGATTGA AAGAATATTG GATACTGGTA TCAGTGTCAG GAAAAGAGTA
ATAAAGATTC TCAGAGACAT TTGTATTGAA CAACCAACAT TTCCAAAAAT CACAGAAATG
TGTGTAAAAA TGATTCGCAG AGTCAATGAT GAAGAGGGCA TTAAGAAATT AGTAAATGAA
ACATTCCAGA AACTCTGGTT TACTCCAACT CCACACAATG ACAAAGAAGC AATGACAAGG
AAAATTTTAA ACATTACCGA TGTGGTTGCA GCATGCAGAG ATACTGGATA TGACTGGTTT
GAGCAACTGC TTCAAAACTT GTTGAAGTCC GAAGAGGATT CCTCATATAA ACCTGTGAAG
AAAGCTTGTA CTCAACTTGT TGATAACCTA GTTGAGCACA TTCTTAAATA TGAGGAATCT
CTAGCTGACT CTGACAATAA AGGTGTGAAT TCTGGAAGAT TGGTAGCTTG CATAACCACT
TTGTTCTTAT TCAGCAAAAT AAGACCCCAG CTCATGGTTA AACATGCAAT GACTATGCAA
CCATACCTTA CCACTAAATG TAGTACGCAA AATGATTTCA TGGTTATCTG CAATGTTGCA
AAAATCCTAG AGCTAGTTGT ACCACTGATG GAGCATCCAA GTGAAACTTT TCTTGCCACT
ATTGAGGAAG ATCTAATGAA GCTCATCATC AAATATGGCA TGACTGTAGT GCAACATTGT
GTGAGCTGTC TTGGAGCTGT TGTAAATAAA GTGACACAAA ATTTTAAATT TGTGTGGGCT
TGTTTCAATA GATACTATGG TGCCATTTCA AAATTAAAAA GTCAACACCA AGAGGACCCA
AATAACACTT CACTTCTAAC AAACAAACCA GCACTTCTTA GATCCCTTTT CACCGTTGGA
GCACTATGTC GGCATTTTGA TTTTGATCTG GAAGATTTTA AAGGCAACAG CAAGGTTAAC
ATAAAAGATA AAGTACTTGA ACTATTGATG TATTTTACAA AACACTCAGA TGAAGAAGTA
CAAACAAAAG CTATCATTGG TCTAGGATTT GCCTTTATTC AGCATCCAAG TCTAATGTTC
GAGCAAGAAG TGAAGAATCT ATATAATAAT ATTTTATCTG ATAAGAACTC CTCAGTCAAT
TTAAAAATAC AAGTGTTAAA AAACCTCCAG ACCTACCTAC AAGAAGAAGA TACACGTATG
CAGCAGGCAG ATAGAGACTG GAAGAAAGTT GCAAAACAGG AAGACTTAAA AGAAATGGGT
GATGTTTCCT CAGGGATGAG TAGTTCCATC ATGCAGCTTT ATCTCAAACA GGTGCTTGAG
GCATTTTTTC ACACCCAGTC AAGTGTACGC CACTTTGCCC TAAATGTCAT TGCATTGACT
CTAAATCAAG GTCTTATTCA TCCAGTTCAG TGTGTGCCAT ATTTAATTGC TATGGGCACA
GACCCAGAAC CTGCTATGCG GAACAAGGCT GATCAGCAAC TTGTGGAAAT AGACAAAAAA
TATGCTGGAT TCATTCATAT GAAAGCAGTG GCTGGTATGA AGATGTCTTA CCAGGTACAA
CAGGCAATCA ACACATGCCT AAAAGATCCT GTAAGGGGTT TCAGACAAGA CGAGTCCTCT
AGCGCTTTGT GTTCACACCT TTACTCCATG ATCCGTGGAA ACCGCCAACA CAGACGAGCC
TTTCTTATTT CTTTACTCAA CCTCTTTGAT GACACAGCAA AAACAGACGT GACTATGCTC
TTGTATATAG CAGACAATCT AGCCTGTTTT CCATACCAGA CACAGGAAGA GCCGTTGTTT
ATAATGCATC ATATAGACAT TACACTCTCA GTTTCTGGTA GTAACCTACT GCAGTCATTC
AAGGAGTCTA TGGTAAAGGA CAAAAGGAAA GAGAGAAAAT CATCACCTAG TAAGGAAAAT
GAGTCAAGCG ACAGTGAAGA AGAAGTTTCC AGGCCTCGGA AGTCACGGAA ACGTGTAGAT
TCAGATTCAG ATTCAGATTC AGAAGACGAT ATAAATTCAG TGATGAAATG TTTGCCAGAA
AATTCAGCTC CTTTAATCGA ATTTGCAAAT GTGTCCCAGG GTATTTTATT ACTTCTCATG
TTAAAACAAC ATTTGAAGAA TCTTTGTGGA TTTTCTGATA GTAAAATTCA GAAGTACTCT
CCATCTGAAT CTGCAAAAGT ATATGATAAA GCGATAAACC GAAAAACAGG AGTTCATTTT
CATCCAAAAC AAACACTGGA CTTCCTGCGG AGTGACATGG CTAATTCCAA AATCACAGAA
GAGGTGAAAA GGAGTATAGT AAAACAGTAT CTAGATTTCA AACTTCTCAT GGAACATCTG
GACCCTGATG AAGAAGAAGA AGAAGGGGAG GTTTCAGCTA GCACAAATGC TCGGAACAAA
GCAATTACCT CACTGCTTGG AGGAGGCAGC CCTAAAAATA ATACAGCAGC AGAGACAGAA
GATGATGAAA GTGATGGGGA GGATAGAGGA GGAGGCACTT CAGGGTCATT GAGAAGGTCA
AAACGAAATT CAGACTCTAC GGAGTTGGCA GCACAGATGA ATGAAAGTGT TGACGTCATG
GATGTCATCG CTATTTGCTG TCCAAAGTAC AAAGATCGAC CACAAATTGC AAGAGTAGTG
CAGAAAACCA GCAGTGGCTT CAGTGTTCAG TGGATGGCAG GCTCCTACAG TGGCTCCTGG
ACTGAGGCTA AGCGCCGTGA TGGCCGCAAA CTGGTGCCTT GGGTAGACAC TATTAAAGAG
TCAGACATTA TTTACAAAAA AATTGCTCTA ACGAGTGCTA ATAAGCTGAC TAATAAAGTT
GTTCAGACTT TACGATCCCT GTATGCCGCC AAGGATGGGA CTTCCAGCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248339.2
CDS72..7742
Misc Feature(1)2586..2810(+)
Misc Feature(2)4707..4832(+)
Misc Feature(3)6147..6698(+)
Translation

Target ORF information:

RefSeq Version XM_005248282
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248282

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
ATGCTTTTGA ATTCCAACAC TTTACCTGAT GGAGATTCTT CAACAATGAG GAATGCTGCA 
TCTTTTCCCT TGAGATCTCC ACAGCCAGTA TGCTCCCCTG CTGGAAGTGA AGGAACTCCT
AAAGGCTCAA GACCACCTTT AATCCTACAA TCTCAGTCTC TACCTTGTTC ATCACCTCGA
GATGTTCCAC CAGATATCTT GCTAGATTCT CCAGAAAGAA AACAAAAGAA GCAGAAGAAA
ATGAAATTAG GCAAGGATGA AAAAGAGCAG AGTGAGAAAG CGGCAATGTA TGATATAATT
AGTTCTCCAT CCAAGGACTC TACTAAACTT ACATTAAGAC TTTCTCGTGT AAGGTCTTCA
GACATGGACC AGCAAGAGGA TATGATTTCT GGTGTGGAAA ATAGCAATGT TTCAGAAAAT
GATATTCCTT TTAATGTGCA GTACCCAGGA CAGACTTCAA AAACACCCAT TACTCCACAA
GATATAAACC GCCCACTAAA TGCTGCTCAA TGTTTGTCGC AGCAAGAACA AACAGCATTC
CTTCCAGCAA ATCAAGTGCC TGTTTTACAA CAGAACACTT CAGTTGCTGC AAAACAACCC
CAGACTTCTG TGGTACAGAA TCAACAACAG ATATCACAAC AGGGACCTAT ATATGATGAA
GTGGAATTGG ATGCATTGGC TGAAATTGAG CGAATAGAGA GAGAATCAGC TATTGAAAGG
GAGCGCTTCT CAAAAGAAGT TCAAGATAAA GATAAGCCTT TGAAAAAAAG AAAACAAGAT
TCTTACCCAC AGGAGGCTGG GGGTGCTACA GGAGGTAATA GACCAGCTTC TCAGGAGACG
GGTTCTACGG GAAATGGGTC AAGGCCAGCA TTAATGGTTA GCATTGATCT TCATCAGGCA
GGAAGAGTGG ACTCTCAGGC TTCTATAACT CAGGATTCAG ACTCCATAAA AAAGCCTGAA
GAAATCAAAC AATGTAATGA TGCACCTGTT TCTGTTCTTC AGGAAGATAT TGTTGGAAGT
CTTAAATCTA CACCAGAAAA CCATCCTGAG ACACCTAAAA AAAAGTCTGA TCCTGAGCTT
TCAAAGAGTG AAATGAAACA AAGTGAAAGT AGATTAGCAG AATCTAAACC AAATGAAAAC
CGATTGGTGG AGACAAAATC AAGTGAAAAT AAGTTAGAAA CTAAAGTTGA GACCCAAACA
GAAGAACTTA AACAGAATGA GAGCAGAACA ACTGAATGCA AACAAAACGA GAGCACCATA
GTTGAGCCTA AACAAAATGA AAATAGACTG TCTGACACAA AACCAAATGA CAACAAACAA
AATAATGGCA GATCAGAAAC AACAAAATCA AGGCCTGAAA CCCCAAAGCA AAAGGGTGAA
AGCCGGCCTG AGACTCCAAA ACAAAAGAGT GATGGGCATC CTGAAACCCC AAAACAGAAG
GGTGATGGAA GGCCTGAAAC TCCAAAGCAA AAAGGTGAGA GCCGCCCTGA AACTCCAAAG
CAAAAAAATG AAGGGCGACC TGAAACACCA AAACACAGGC ATGACAATAG GAGGGATTCT
GGAAAGCCAT CTACAGAGAA AAAACCTGAA GTGTCTAAAC ATAAACAAGA TACTAAATCT
GACTCACCTC GGTTAAAATC AGAACGAGCT GAAGCCTTAA AGCAGAGACC TGATGGGCGA
TCTGTTTCTG AGTCACTAAG ACGTGACCAT GATAATAAAC AAAAATCAGA TGACAGGGGT
GAATCAGAGC GACATCGAGG GGATCAGTCT AGGGTTCGAA GACCAGAAAC ATTGAGATCC
TCTAGTAGAA ATGAACATGG CATTAAATCT GATAGTTCAA AAACTGATAA ACTAGAACGA
AAACACAGGC ATGAATCAGG GGACTCAAGG GAAAGACCAT CTTCTGGGGA ACAAAAATCA
AGACCTGACA GTCCTCGTGT TAAACAAGGA GATTCTAATA AATCAAGATC TGATAAACTT
GGTTTTAAAT CACCAACTAG TAAAGATGAC AAAAGGACAG AGGGTAACAA GAGTAAAGTA
GACACTAATA AAGCACACCC TGACAATAAG GCAGAATTTC CAAGTTATTT GTTGGGGGGC
AGGTCTGGTG CGTTGAAAAA TTTTGTCATT CCGAAAATCA AGAGGGATAA AGATGGCAAT
GTTACTCAGG AGACAAAGAA AATGGAAATG AAAGGAGAGC CGAAAGACAA AGTAGAAAAA
ATAGGATTAG TTGAAGATCT AAATAAAGGA GCTAAGCCTG TAGTTGTGCT ACAAAAACTG
TCTTTGGATG ATGTTCAGAA ACTTATTAAA GATAGAGAGG ACAAATCAAG AAGTTCCCTT
AAACCTATCA AGAATAAACC ATCAAAGTCA AATAAAGGTA GTATAGATCA ATCAGTGTTA
AAAGAATTAC CCCCTGAACT CCTGGCAGAA ATTGAGTCCA CCATGCCACT TTGTGAACGT
GTGAAAATGA ACAAACGCAA GCGTAGCACA GTTAATGAAA AGCCAAAATA TGCTGAAATC
AGTTCAGATG AAGATAATGA TAGTGATGAA GCTTTTGAAT CCTCTAGGAA ACGACATAAA
AAAGATGATG ATAAAGCTTG GGAATATGAA GAGCGTGACA GAAGAAGCTC TGGGGATCAT
AGGAGAAGTG GCCACTCTCA TGAAGGAAGA AGGAGTTCAG GTGGTGGTCG TTATCGAAAC
CGAAGTCCGT CAGATTCTGA CATGGAAGAT TATTCTCCTC CTCCCAGCCT TAGTGAGGTT
GCTAGGAAAA TGAAGAAAAA AGAAAAACAG AAGAAAAGGA AAGCATATGA ACCAAAACTA
ACACCTGAAG AAATGATGGA CTCTTCAACT TTTAAGAGAT TCACAGCCTC AATAGAGAAT
ATTTTGGATA ATTTGGAAGA TATGGATTTT ACTGCGTTTG GTGATGATGA TGAAATTCCT
CAGGAACTGC TCTTAGGAAA ACATCAGCTT AATGAACTTG GCAGTGAATC TGCTAAAATA
AAAGCAATGG GTATAATGGA TAAGCTTTCA ACTGACAAAA CTGTGAAAGT CTTAAATATC
TTGGAGAAGA ATATTCAGGA TGGGTCAAAG CTTTCCACTT TGTTAAATCA TAATAACGAT
ACTGAAGAAG AAGAAAGGTT ATGGAGAGAC CTTATTATGG AGAGAGTTAC AAAATCAGCG
GATGCTTGTC TTACAACTAT CAACATTATG ACATCCCCTA ACATGCCAAA AGCTGTGTAC
ATTGAGGATG TAATTGAAAG AGTTATACAG TACACTAAAT TTCATTTGCA GAATACACTT
TATCCTCAGT ATGATCCTGT TTACAGATTA GATCCTCATG GAGGAGGCTT ATTAAGTTCA
AAAGCAAAAC GGGCTAAATG TTCTACCCAT AAGCAGAGAG TAATAGTAAT GCTTTATAAC
AAAGTTTGTG ACATTGTTAG CAGCTTATCA GAATTGCTAG AGATACAACT TCTTACAGAC
ACAACAATTC TTCAGGTTTC ATCTATGGGA ATAACACCAT TTTTTGTGGA AAATGTCAGT
GAACTACAGT TGTGTGCCAT TAAGTTAGTC ACTGCAGTAT TCTCAAGATA TGAAAAACAT
AGGCAGTTAA TTTTGGAAGA AATTTTTACT TCACTTGCAA GATTACCAAC CAGCAAGAGG
AGTTTAAGGA ACTTCAGGTT AAACAGTAGT GATATGGATG GAGAACCTAT GTATATTCAG
ATGGTTACAG CACTGGTTTT ACAACTTATT CAGTGTGTGG TACACTTACC ATCATCAGAG
AAGGACTCTA ATGCAGAAGA AGATTCAAAT AAAAAAATTG ACCAGGATGT TGTCATTACT
AACTCTTATG AAACAGCTAT GCGAACAGCC CAAAACTTCC TCTCCATCTT CCTTAAAAAA
TGTGGTAGTA AGCAAGGTGA AGAAGATTAC AGACCACTGT TTGAAAATTT TGTTCAAGAC
CTTCTTTCAA CAGTCAATAA GCCTGAATGG CCAGCTGCTG AACTACTCCT TAGTTTGTTA
GGGAGACTGT TGGTTCATCA GTTCAGTAAC AAGTCAACAG AGATGGCTTT AAGAGTGGCA
TCTCTTGATT ACCTTGGAAC TGTTGCTGCA CGGCTAAGAA AAGATGCTGT TACAAGCAAA
ATGGATCAAG GATCTATAGA ACGCATTTTA AAACAGGTTT CAGGAGGGGA AGATGAAATC
CAACAATTAC AAAAAGCATT GCTTGATTAC TTGGATGAAA ACACTGAGAC TGATCCTTCA
CTAGTGTTTT CTCGTAAATT CTATATAGCC CAGTGGTTTC GAGACACAAC TCTGGAAACA
GAAAAAGCAA TGAAATCACA AAAAGATGAA GAATCATCTG AAGGAACACA TCATGCAAAG
GAAATTGAGA CAACTGGCCA AATTATGCAT CGAGCTGAAA ACCGAAAAAA GTTTCTTAGA
AGCATTATCA AAACCACACC TTCTCAGTTT AGCACATTAA AGATGAACTC TGATACTGTG
GACTATGATG ATGCTTGCTT GATTGTTCGA TACTTGGCCT CCATGAGGCC GTTTGCCCAG
AGCTTTGATA TTTATTTGAC ACAGATCCTA CGAGTTCTTG GTGAAAATGC AATTGCTGTT
CGAACAAAAG CCATGAAGTG TTTGTCTGAG GTTGTTGCTG TAGACCCCAG TATTCTAGCA
AGGCTTGATA TGCAACGAGG TGTTCATGGA CGATTGATGG ATAATTCGAC TAGTGTCCGA
GAAGCAGCAG TAGAATTACT AGGTCGATTT GTCCTTTGTC GACCTCAGCT TGCTGAACAG
TATTATGATA TGCTGATTGA AAGAATATTG GATACTGGTA TCAGTGTCAG GAAAAGAGTA
ATAAAGATTC TCAGAGACAT TTGTATTGAA CAACCAACAT TTCCAAAAAT CACAGAAATG
TGTGTAAAAA TGATTCGCAG AGTCAATGAT GAAGAGGGCA TTAAGAAATT AGTAAATGAA
ACATTCCAGA AACTCTGGTT TACTCCAACT CCACACAATG ACAAAGAAGC AATGACAAGG
AAAATTTTAA ACATTACCGA TGTGGTTGCA GCATGCAGAG ATACTGGATA TGACTGGTTT
GAGCAACTGC TTCAAAACTT GTTGAAGTCC GAAGAGGATT CCTCATATAA ACCTGTGAAG
AAAGCTTGTA CTCAACTTGT TGATAACCTA GTTGAGCACA TTCTTAAATA TGAGGAATCT
CTAGCTGACT CTGACAATAA AGGTGTGAAT TCTGGAAGAT TGGTAGCTTG CATAACCACT
TTGTTCTTAT TCAGCAAAAT AAGACCCCAG CTCATGGTTA AACATGCAAT GACTATGCAA
CCATACCTTA CCACTAAATG TAGTACGCAA AATGATTTCA TGGTTATCTG CAATGTTGCA
AAAATCCTAG AGCTAGTTGT ACCACTGATG GAGCATCCAA GTGAAACTTT TCTTGCCACT
ATTGAGGAAG ATCTAATGAA GCTCATCATC AAATATGGCA TGACTGTAGT GCAACATTGT
GTGAGCTGTC TTGGAGCTGT TGTAAATAAA GTGACACAAA ATTTTAAATT TGTGTGGGCT
TGTTTCAATA GATACTATGG TGCCATTTCA AAATTAAAAA GTCAACACCA AGAGGACCCA
AATAACACTT CACTTCTAAC AAACAAACCA GCACTTCTTA GATCCCTTTT CACCGTTGGA
GCACTATGTC GGCATTTTGA TTTTGATCTG GAAGATTTTA AAGGCAACAG CAAGGTTAAC
ATAAAAGATA AAGTACTTGA ACTATTGATG TATTTTACAA AACACTCAGA TGAAGAAGTA
CAAACAAAAG CTATCATTGG TCTAGGATTT GCCTTTATTC AGCATCCAAG TCTAATGTTC
GAGCAAGAAG TGAAGAATCT ATATAATAAT ATTTTATCTG ATAAGAACTC CTCAGTCAAT
TTAAAAATAC AAGTGTTAAA AAACCTCCAG ACCTACCTAC AAGAAGAAGA TACACGTATG
CAGCAGGCAG ATAGAGACTG GAAGAAAGTT GCAAAACAGG AAGACTTAAA AGAAATGGGT
GATGTTTCCT CAGGGATGAG TAGTTCCATC ATGCAGCTTT ATCTCAAACA GGTGCTTGAG
GCATTTTTTC ACACCCAGTC AAGTGTACGC CACTTTGCCC TAAATGTCAT TGCATTGACT
CTAAATCAAG GTCTTATTCA TCCAGTTCAG TGTGTGCCAT ATTTAATTGC TATGGGCACA
GACCCAGAAC CTGCTATGCG GAACAAGGCT GATCAGCAAC TTGTGGAAAT AGACAAAAAA
TATGCTGGAT TCATTCATAT GAAAGCAGTG GCTGGTATGA AGATGTCTTA CCAGGTACAA
CAGGCAATCA ACACATGCCT AAAAGATCCT GTAAGGGGTT TCAGACAAGA CGAGTCCTCT
AGCGCTTTGT GTTCACACCT TTACTCCATG ATCCGTGGAA ACCGCCAACA CAGACGAGCC
TTTCTTATTT CTTTACTCAA CCTCTTTGAT GACACAGCAA AAACAGACGT GACTATGCTC
TTGTATATAG CAGACAATCT AGCCTGTTTT CCATACCAGA CACAGGAAGA GCCGTTGTTT
ATAATGCATC ATATAGACAT TACACTCTCA GTTTCTGGTA GTAACCTACT GCAGTCATTC
AAGGAGTCTA TGGTAAAGGA CAAAAGGAAA GAGAGAAAAT CATCACCTAG TAAGGAAAAT
GAGTCAAGCG ACAGTGAAGA AGAAGTTTCC AGGCCTCGGA AGTCACGGAA ACGTGTAGAT
TCAGATTCAG ATTCAGATTC AGAAGACGAT ATAAATTCAG TGATGAAATG TTTGCCAGAA
AATTCAGCTC CTTTAATCGA ATTTGCAAAT GTGTCCCAGG GTATTTTATT ACTTCTCATG
TTAAAACAAC ATTTGAAGAA TCTTTGTGGA TTTTCTGATA GTAAAATTCA GAAGTACTCT
CCATCTGAAT CTGCAAAAGT ATATGATAAA GCGATAAACC GAAAAACAGG AGTTCATTTT
CATCCAAAAC AAACACTGGA CTTCCTGCGG AGTGACATGG CTAATTCCAA AATCACAGAA
GAGGTGAAAA GGAGTATAGT AAAACAGTAT CTAGATTTCA AACTTCTCAT GGAACATCTG
GACCCTGATG AAGAAGAAGA AGAAGGGGAG GTTTCAGCTA GCACAAATGC TCGGAACAAA
GCAATTACCT CACTGCTTGG AGGAGGCAGC CCTAAAAATA ATACAGCAGC AGAGACAGAA
GATGATGAAA GTGATGGGGA GGATAGAGGA GGAGGCACTT CAGGGTCATT GAGAAGGTCA
AAACGAAATT CAGACTCTAC GGAGTTGGCA GCACAGATGA ATGAAAGTGT TGACGTCATG
GATGTCATCG CTATTTGCTG TCCAAAGTAC AAAGATCGAC CACAAATTGC AAGAGTAGTG
CAGAAAACCA GCAGTGGCTT CAGTGTTCAG TGGATGGCAG GCTCCTACAG TGGCTCCTGG
ACTGAGGCTA AGCGCCGTGA TGGCCGCAAA CTGGTGCCTT GGGTAGACAC TATTAAAGAG
TCAGACATTA TTTACAAAAA AATTGCTCTA ACGAGTGCTA ATAAGCTGAC TAATAAAGTT
GTTCAGACTT TACGATCCCT GTATGCCGCC AAGGATGGGA CTTCCAGCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu66530
Clone ID Related Accession (Same CDS sequence) XM_011514015
Accession Version XM_011514015.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512317.1
CDS489..7754
Misc Feature(1)3747..3971(+)
Misc Feature(2)5868..5993(+)
Misc Feature(3)7308..>7745(+)
Translation

Target ORF information:

RefSeq Version XM_011514015
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514015

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26171
Clone ID Related Accession (Same CDS sequence) NM_133433
Accession Version NM_133433.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8415bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced gi:47578104. Transcript Variant: This variant (A) encodes the longer isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ627032.1, BK005151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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121
181
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301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_597677.2
CDS500..8914
Misc Feature(1)473..475(+)
Misc Feature(2)947..949(+)
Misc Feature(3)983..985(+)
Misc Feature(4)1265..1267(+)
Misc Feature(5)1412..1414(+)
Misc Feature(6)1415..1417(+)
Misc Feature(7)1451..1453(+)
Misc Feature(8)1451..1453(+)
Misc Feature(9)1547..1549(+)
Misc Feature(10)1547..1549(+)
Misc Feature(11)1631..1633(+)
Misc Feature(12)2435..2437(+)
Misc Feature(13)2636..2638(+)
Misc Feature(14)2636..2638(+)
Misc Feature(15)2735..2737(+)
Misc Feature(16)3044..3046(+)
Misc Feature(17)3047..3049(+)
Misc Feature(18)3119..3121(+)
Misc Feature(19)3128..3130(+)
Misc Feature(20)3233..3235(+)
Misc Feature(21)3485..3526(+)
Misc Feature(22)3752..3754(+)
Misc Feature(23)3758..3982(+)
Misc Feature(24)3764..3766(+)
Misc Feature(25)3764..3766(+)
Misc Feature(26)3767..3769(+)
Misc Feature(27)3767..3769(+)
Misc Feature(28)3785..3787(+)
Misc Feature(29)3785..3787(+)
Misc Feature(30)3947..3949(+)
Misc Feature(31)3953..3955(+)
Misc Feature(32)3959..3961(+)
Misc Feature(33)3977..3979(+)
Misc Feature(34)5798..5914(+)
Misc Feature(35)5879..6004(+)
Misc Feature(36)6026..6142(+)
Misc Feature(37)6332..6451(+)
Misc Feature(38)7178..7300(+)
Misc Feature(39)7319..7870(+)
Misc Feature(40)7436..7552(+)
Misc Feature(41)8024..8026(+)
Misc Feature(42)8030..8032(+)
Misc Feature(43)8036..8038(+)
Misc Feature(44)8042..8044(+)
Misc Feature(45)8471..8473(+)
Misc Feature(46)8471..8473(+)
Misc Feature(47)8498..8500(+)
Misc Feature(48)8513..8515(+)
Exon (1)1..420
Gene:NIPBL
Gene Synonym:
Exon (2)421..563
Gene:NIPBL
Gene Synonym:
Exon (3)564..729
Gene:NIPBL
Gene Synonym:
Exon (4)730..857
Gene:NIPBL
Gene Synonym:
Exon (5)858..957
Gene:NIPBL
Gene Synonym:
Exon (6)958..1109
Gene:NIPBL
Gene Synonym:
Exon (7)1110..1270
Gene:NIPBL
Gene Synonym:
Exon (8)1271..1367
Gene:NIPBL
Gene Synonym:
Exon (9)1368..1994
Gene:NIPBL
Gene Synonym:
Exon (10)1995..3620
Gene:NIPBL
Gene Synonym:
Exon (11)3621..3803
Gene:NIPBL
Gene Synonym:
Exon (12)3804..4001
Gene:NIPBL
Gene Synonym:
Exon (13)4002..4073
Gene:NIPBL
Gene Synonym:
Exon (14)4074..4163
Gene:NIPBL
Gene Synonym:
Exon (15)4164..4267
Gene:NIPBL
Gene Synonym:
Exon (16)4268..4354
Gene:NIPBL
Gene Synonym:
Exon (17)4355..4586
Gene:NIPBL
Gene Synonym:
Exon (18)4587..4738
Gene:NIPBL
Gene Synonym:
Exon (19)4739..4819
Gene:NIPBL
Gene Synonym:
Exon (20)4820..4920
Gene:NIPBL
Gene Synonym:
Exon (21)4921..5059
Gene:NIPBL
Gene Synonym:
Exon (22)5060..5142
Gene:NIPBL
Gene Synonym:
Exon (23)5143..5275
Gene:NIPBL
Gene Synonym:
Exon (24)5276..5419
Gene:NIPBL
Gene Synonym:
Exon (25)5420..5509
Gene:NIPBL
Gene Synonym:
Exon (26)5510..5724
Gene:NIPBL
Gene Synonym:
Exon (27)5725..5827
Gene:NIPBL
Gene Synonym:
Exon (28)5828..5926
Gene:NIPBL
Gene Synonym:
Exon (29)5927..6073
Gene:NIPBL
Gene Synonym:
Exon (30)6074..6208
Gene:NIPBL
Gene Synonym:
Exon (31)6209..6307
Gene:NIPBL
Gene Synonym:
Exon (32)6308..6361
Gene:NIPBL
Gene Synonym:
Exon (33)6362..6470
Gene:NIPBL
Gene Synonym:
Exon (34)6471..6607
Gene:NIPBL
Gene Synonym:
Exon (35)6608..6748
Gene:NIPBL
Gene Synonym:
Exon (36)6749..6842
Gene:NIPBL
Gene Synonym:
Exon (37)6843..6997
Gene:NIPBL
Gene Synonym:
Exon (38)6998..7088
Gene:NIPBL
Gene Synonym:
Exon (39)7089..7262
Gene:NIPBL
Gene Synonym:
Exon (40)7263..7453
Gene:NIPBL
Gene Synonym:
Exon (41)7454..7561
Gene:NIPBL
Gene Synonym:
Exon (42)7562..7762
Gene:NIPBL
Gene Synonym:
Exon (43)7763..7909
Gene:NIPBL
Gene Synonym:
Exon (44)7910..8184
Gene:NIPBL
Gene Synonym:
Exon (45)8185..8359
Gene:NIPBL
Gene Synonym:
Exon (46)8360..8548
Gene:NIPBL
Gene Synonym:
Exon (47)8549..9841
Gene:NIPBL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_133433
Organism Homo sapiens (human)
Definition Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_133433

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26399
Clone ID Related Accession (Same CDS sequence) NM_015384
Accession Version NM_015384.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8094bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nipped-B-like protein isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1, AJ640137.1, AA508213.1, BQ723324.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced gi:47578106. Transcript Variant: This variant (B) is alternatively spliced at the 3' end compared to transcript variant A. It encodes an isoform (B) with a shorter, distinct C-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ640137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_056199.2
CDS500..8593
Misc Feature(1)473..475(+)
Misc Feature(2)947..949(+)
Misc Feature(3)983..985(+)
Misc Feature(4)1265..1267(+)
Misc Feature(5)1412..1414(+)
Misc Feature(6)1415..1417(+)
Misc Feature(7)1451..1453(+)
Misc Feature(8)1451..1453(+)
Misc Feature(9)1547..1549(+)
Misc Feature(10)1547..1549(+)
Misc Feature(11)1631..1633(+)
Misc Feature(12)2435..2437(+)
Misc Feature(13)2636..2638(+)
Misc Feature(14)2636..2638(+)
Misc Feature(15)2735..2737(+)
Misc Feature(16)3044..3046(+)
Misc Feature(17)3047..3049(+)
Misc Feature(18)3119..3121(+)
Misc Feature(19)3128..3130(+)
Misc Feature(20)3233..3235(+)
Misc Feature(21)3485..3526(+)
Misc Feature(22)3752..3754(+)
Misc Feature(23)3758..3982(+)
Misc Feature(24)3764..3766(+)
Misc Feature(25)3764..3766(+)
Misc Feature(26)3767..3769(+)
Misc Feature(27)3767..3769(+)
Misc Feature(28)3785..3787(+)
Misc Feature(29)3785..3787(+)
Misc Feature(30)3947..3949(+)
Misc Feature(31)3953..3955(+)
Misc Feature(32)3959..3961(+)
Misc Feature(33)3977..3979(+)
Misc Feature(34)5798..5914(+)
Misc Feature(35)5879..6004(+)
Misc Feature(36)6026..6142(+)
Misc Feature(37)6332..6451(+)
Misc Feature(38)7178..7300(+)
Misc Feature(39)7319..7870(+)
Misc Feature(40)7436..7552(+)
Misc Feature(41)8024..8026(+)
Misc Feature(42)8030..8032(+)
Misc Feature(43)8036..8038(+)
Misc Feature(44)8042..8044(+)
Misc Feature(45)8471..8473(+)
Misc Feature(46)8471..8473(+)
Misc Feature(47)8498..8500(+)
Misc Feature(48)8513..8515(+)
Exon (1)1..420
Gene:NIPBL
Gene Synonym:
Exon (2)421..563
Gene:NIPBL
Gene Synonym:
Exon (3)564..729
Gene:NIPBL
Gene Synonym:
Exon (4)730..857
Gene:NIPBL
Gene Synonym:
Exon (5)858..957
Gene:NIPBL
Gene Synonym:
Exon (6)958..1109
Gene:NIPBL
Gene Synonym:
Exon (7)1110..1270
Gene:NIPBL
Gene Synonym:
Exon (8)1271..1367
Gene:NIPBL
Gene Synonym:
Exon (9)1368..1994
Gene:NIPBL
Gene Synonym:
Exon (10)1995..3620
Gene:NIPBL
Gene Synonym:
Exon (11)3621..3803
Gene:NIPBL
Gene Synonym:
Exon (12)3804..4001
Gene:NIPBL
Gene Synonym:
Exon (13)4002..4073
Gene:NIPBL
Gene Synonym:
Exon (14)4074..4163
Gene:NIPBL
Gene Synonym:
Exon (15)4164..4267
Gene:NIPBL
Gene Synonym:
Exon (16)4268..4354
Gene:NIPBL
Gene Synonym:
Exon (17)4355..4586
Gene:NIPBL
Gene Synonym:
Exon (18)4587..4738
Gene:NIPBL
Gene Synonym:
Exon (19)4739..4819
Gene:NIPBL
Gene Synonym:
Exon (20)4820..4920
Gene:NIPBL
Gene Synonym:
Exon (21)4921..5059
Gene:NIPBL
Gene Synonym:
Exon (22)5060..5142
Gene:NIPBL
Gene Synonym:
Exon (23)5143..5275
Gene:NIPBL
Gene Synonym:
Exon (24)5276..5419
Gene:NIPBL
Gene Synonym:
Exon (25)5420..5509
Gene:NIPBL
Gene Synonym:
Exon (26)5510..5724
Gene:NIPBL
Gene Synonym:
Exon (27)5725..5827
Gene:NIPBL
Gene Synonym:
Exon (28)5828..5926
Gene:NIPBL
Gene Synonym:
Exon (29)5927..6073
Gene:NIPBL
Gene Synonym:
Exon (30)6074..6208
Gene:NIPBL
Gene Synonym:
Exon (31)6209..6307
Gene:NIPBL
Gene Synonym:
Exon (32)6308..6361
Gene:NIPBL
Gene Synonym:
Exon (33)6362..6470
Gene:NIPBL
Gene Synonym:
Exon (34)6471..6607
Gene:NIPBL
Gene Synonym:
Exon (35)6608..6748
Gene:NIPBL
Gene Synonym:
Exon (36)6749..6842
Gene:NIPBL
Gene Synonym:
Exon (37)6843..6997
Gene:NIPBL
Gene Synonym:
Exon (38)6998..7088
Gene:NIPBL
Gene Synonym:
Exon (39)7089..7262
Gene:NIPBL
Gene Synonym:
Exon (40)7263..7453
Gene:NIPBL
Gene Synonym:
Exon (41)7454..7561
Gene:NIPBL
Gene Synonym:
Exon (42)7562..7762
Gene:NIPBL
Gene Synonym:
Exon (43)7763..7909
Gene:NIPBL
Gene Synonym:
Exon (44)7910..8184
Gene:NIPBL
Gene Synonym:
Exon (45)8185..8359
Gene:NIPBL
Gene Synonym:
Exon (46)8360..10389
Gene:NIPBL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_015384
Organism Homo sapiens (human)
Definition Homo sapiens Nipped-B homolog (Drosophila) (NIPBL), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015384

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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