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GAPDH glyceraldehyde-3-phosphate dehydrogenase [Homo sapiens (human)]

Gene Symbol GAPDH
Entrez Gene ID 2597
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, HEL-S-162eP
General protein information
Preferred Names
glyceraldehyde-3-phosphate dehydrogenase
Names
glyceraldehyde-3-phosphate dehydrogenase
aging-associated gene 9 protein
peptidyl-cysteine S-nitrosylase GAPDH
NP_001243728.1
NP_002037.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13

Summary This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

138400

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu20566 NM_002046 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu20465 NM_001256799 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu20566 NM_001289746 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu20566 NM_001289745 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu20566D
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from HY046784.1, BC009081.1 and BE893087.1. This sequence is a reference standard in the RefSeqGene project. On Jan 28, 2014 this sequence version replaced gi:378404906. Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 Protein has antimicrobial activity :: PMID: 22832495 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AF261085.1, BC013310.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_002037.2
CDS189..1196
Misc Feature(1)192..632(+)
Misc Feature(2)195..1193(+)
Misc Feature(3)198..644(+)
Misc Feature(4)201..203(+)
Misc Feature(5)213..215(+)
Misc Feature(6)312..314(+)
Misc Feature(7)312..314(+)
Misc Feature(8)369..371(+)
Misc Feature(9)378..380(+)
Misc Feature(10)384..386(+)
Misc Feature(11)396..398(+)
Misc Feature(12)411..413(+)
Misc Feature(13)435..437(+)
Misc Feature(14)435..437(+)
Misc Feature(15)552..554(+)
Misc Feature(16)630..632(+)
Misc Feature(17)633..635(+)
Misc Feature(18)639..647(+)
Misc Feature(19)639..641(+)
Misc Feature(20)642..656(+)
Misc Feature(21)642..644(+)
Misc Feature(22)642..644(+)
Misc Feature(23)642..644(+)
Misc Feature(24)642..644(+)
Misc Feature(25)651..653(+)
Misc Feature(26)657..1130(+)
Misc Feature(27)723..725(+)
Misc Feature(28)738..740(+)
Misc Feature(29)768..770(+)
Misc Feature(30)768..770(+)
Misc Feature(31)768..770(+)
Misc Feature(32)819..824(+)
Misc Feature(33)819..821(+)
Misc Feature(34)831..833(+)
Misc Feature(35)831..833(+)
Misc Feature(36)843..845(+)
Misc Feature(37)861..863(+)
Misc Feature(38)867..869(+)
Misc Feature(39)867..869(+)
Misc Feature(40)873..875(+)
Misc Feature(41)897..899(+)
Misc Feature(42)948..950(+)
Misc Feature(43)966..968(+)
Misc Feature(44)975..977(+)
Misc Feature(45)1122..1124(+)
Misc Feature(46)1134..1136(+)
Misc Feature(47)1188..1190(+)
Exon (1)1..165
Gene:GAPDH
Gene Synonym:
Exon (2)166..217
Gene:GAPDH
Gene Synonym:
Exon (3)218..317
Gene:GAPDH
Gene Synonym:
Exon (4)318..424
Gene:GAPDH
Gene Synonym:
Exon (5)425..515
Gene:GAPDH
Gene Synonym:
Exon (6)516..631
Gene:GAPDH
Gene Synonym:
Exon (7)632..713
Gene:GAPDH
Gene Synonym:
Exon (8)714..1126
Gene:GAPDH
Gene Synonym:
Exon (9)1127..1401
Gene:GAPDH
Gene Synonym:
Translation
Position Chain Variation Link
33 33 c, g dbSNP:7307229
40 40 c, g dbSNP:112427350
41 41 -, g dbSNP:71450157
46 46 c, g dbSNP:780164757
49 49 c, g, t dbSNP:111720901
69 69 c, t dbSNP:45561238
107 107 c, t dbSNP:45571036
109 109 c, t dbSNP:45508900
114 114 c, t dbSNP:564320185
116 116 c, t dbSNP:532940876
133 133 a, g dbSNP:45568532
136 136 a, g dbSNP:559414721
142 142 c, t dbSNP:528502920
143 143 c, t dbSNP:367946267
145 145 c, t dbSNP:527918526
148 148 c, t dbSNP:551180067
150 150 c, t dbSNP:3199979
151 151 c, t dbSNP:11549332
157 157 c, t dbSNP:537766086
166 166 c, t dbSNP:759849225
168 168 c, g dbSNP:772368359
170 170 a, g, t dbSNP:35318117
172 172 c, t dbSNP:372129246
175 175 a, g dbSNP:764146133
177 177 c, t dbSNP:753709203
181 181 c, g dbSNP:1136666
185 185 c, t dbSNP:764733553
190 190 c, t dbSNP:147280944
192 192 a, g dbSNP:758070479
198 198 a, g dbSNP:367765818
199 199 c, t dbSNP:369545182
203 203 c, g dbSNP:371408635
204 204 a, g dbSNP:778410218
208 208 c, g dbSNP:749762041
209 209 a, c dbSNP:771409071
215 215 c, t dbSNP:530766121
224 224 c, t dbSNP:750965191
234 234 c, t dbSNP:758911385
248 248 a, g dbSNP:190547766
253 253 c, g dbSNP:45541435
263 263 c, t dbSNP:747064609
274 274 a, g dbSNP:11549361
276 276 a, g dbSNP:755187648
285 285 a, g dbSNP:376494973
290 290 a, t dbSNP:747986504
293 293 c, t dbSNP:769573085
294 294 c, g dbSNP:11549369
296 296 c, t dbSNP:140793249
299 299 c, t dbSNP:534209743
303 303 g, t dbSNP:11549328
305 305 c, t dbSNP:770803265
310 310 a, g dbSNP:773762261
311 311 c, g, t dbSNP:142283743
318 318 a, g dbSNP:759892796
325 325 c, t dbSNP:767648675
332 332 a, g dbSNP:752723540
338 338 c, t dbSNP:377360455
340 340 a, c dbSNP:200102749
347 347 c, t dbSNP:139204528
350 350 c, t dbSNP:527405745
352 352 a, g dbSNP:757057882
362 362 c, t dbSNP:778786251
364 364 c, t dbSNP:745827298
365 365 c, t dbSNP:771849987
366 366 a, g, t dbSNP:11549351
371 371 a, g dbSNP:746479173
377 377 g, t dbSNP:11549346
380 380 c, t dbSNP:201939440
381 381 a, g dbSNP:776400902
383 383 g, t dbSNP:11549355
390 390 c, g dbSNP:749674237
395 395 c, t dbSNP:771331311
396 396 a, c dbSNP:774521691
397 397 a, g dbSNP:201368443
403 403 a, g dbSNP:767870427
406 406 a, c dbSNP:775584055
416 416 c, t dbSNP:760719504
426 426 c, t dbSNP:755829002
434 434 a, c, g dbSNP:777266705
436 436 c, g, t dbSNP:772417505
445 445 a, g dbSNP:552376556
447 447 g, t dbSNP:113341953
452 452 c, t dbSNP:11549353
461 461 c, t dbSNP:200281741
470 470 c, t dbSNP:11549324
471 471 a, g dbSNP:371192512
473 473 c, t dbSNP:534300550
474 474 a, g dbSNP:770105201
484 484 c, t dbSNP:11549329
488 488 c, t dbSNP:371116598
489 489 a, g dbSNP:149945423
491 491 c, t dbSNP:11549337
494 494 c, t dbSNP:11549363
495 495 a, g dbSNP:751350310
497 497 a, c, t dbSNP:11266742
500 500 c, t dbSNP:759577147
503 503 c, g dbSNP:375963780
506 506 a, g dbSNP:11549374
512 512 c, t dbSNP:752299792
518 518 c, t dbSNP:11549350
521 521 a, t dbSNP:11549322
527 527 a, g dbSNP:760591074
530 530 a, g dbSNP:763523311
532 532 c, g, t dbSNP:11549340
533 533 a, g dbSNP:753299793
536 536 a, c, t dbSNP:11549349
545 545 c, t dbSNP:778509730
551 551 c, t dbSNP:751972977
557 557 c, t dbSNP:758897659
560 560 c, g, t dbSNP:368740142
562 562 c, t dbSNP:11549338
565 565 c, t dbSNP:146516445
566 566 c, t dbSNP:370350073
569 569 c, t dbSNP:1065691
572 572 a, c dbSNP:572265558
575 575 c, t dbSNP:771327842
581 581 c, t dbSNP:774700281
582 582 a, g dbSNP:745721511
584 584 c, t dbSNP:11549336
588 588 a, g dbSNP:771766319
590 590 c, t dbSNP:144761391
593 593 a, g dbSNP:760555162
600 600 a, g dbSNP:764055385
609 609 a, g dbSNP:74407849
614 614 c, t dbSNP:776162625
617 617 c, t dbSNP:761293659
622 622 a, g dbSNP:764928240
623 623 g, t dbSNP:372910824
624 624 a, g, t dbSNP:138863602
625 625 a, t dbSNP:767788286
626 626 c, t dbSNP:753064371
627 627 a, c dbSNP:376053895
635 635 c, t dbSNP:746840589
646 646 c, t dbSNP:11549333
652 652 a, g dbSNP:768495247
656 656 c, t dbSNP:780898155
669 669 g, t dbSNP:77154254
676 676 c, t dbSNP:748092877
682 682 a, g dbSNP:769671313
686 686 c, g dbSNP:772747939
689 689 c, t dbSNP:11549330
690 690 c, t dbSNP:11549326
691 691 g, t dbSNP:762501270
698 698 c, t dbSNP:770546388
699 699 a, g dbSNP:774047959
701 701 a, g dbSNP:562063348
707 707 a, t dbSNP:201481859
708 708 a, c dbSNP:201208495
710 710 c, t dbSNP:11549343
714 714 a, g dbSNP:766493985
726 726 g, t dbSNP:756189036
737 737 c, t dbSNP:369619209
749 749 -, tg dbSNP:747164357
752 752 a, g dbSNP:541601360
761 761 c, t dbSNP:554400197
764 764 c, t dbSNP:373252845
765 765 a, g dbSNP:754017958
767 767 g, t dbSNP:543121368
771 771 c, t dbSNP:376796183
772 772 -, tg dbSNP:778058544
784 784 c, g dbSNP:11549348
788 788 c, t dbSNP:368124080
792 792 c, g dbSNP:11549352
792 792 a, g dbSNP:749257900
795 795 c, t dbSNP:757090796
797 797 a, c, g dbSNP:372768955
800 800 a, g dbSNP:745337926
803 803 c, t dbSNP:771902302
804 804 a, g dbSNP:775271002
815 815 c, t dbSNP:370741285
816 816 c, t dbSNP:11549345
817 817 c, g dbSNP:771140596
821 821 a, t dbSNP:770291627
824 824 c, t dbSNP:773392187
832 832 a, g dbSNP:763539313
842 842 c, t dbSNP:766757138
844 844 a, g dbSNP:774531550
858 858 a, c dbSNP:759596917
863 863 c, t dbSNP:148843886
864 864 a, g dbSNP:752919766
884 884 c, t dbSNP:11549347
884 884 c, g dbSNP:756270449
896 896 a, c dbSNP:11549373
898 898 c, t dbSNP:763967581
899 899 c, t dbSNP:753533688
901 901 c, t dbSNP:757255996
902 902 c, t dbSNP:778691759
905 905 c, t dbSNP:191738457
915 915 g, t dbSNP:757981202
916 916 c, t dbSNP:779648965
930 930 c, t dbSNP:546169843
931 931 a, g dbSNP:768146299
936 936 -, aa dbSNP:749660368
938 938 a, g dbSNP:773658609
941 941 a, c dbSNP:1062429
947 947 c, g dbSNP:749586575
950 950 a, g dbSNP:776921270
952 952 -, atg dbSNP:779337992
952 952 -, a dbSNP:771432837
953 953 c, t dbSNP:771525480
954 954 g, t dbSNP:774833554
960 960 a, g dbSNP:759968940
968 968 -, ggt dbSNP:746549349
971 971 a, g dbSNP:559678529
981 981 a, g dbSNP:377461105
982 982 c, t dbSNP:528329572
983 983 a, g dbSNP:764189570
985 985 c, t dbSNP:11549316
986 986 a, g dbSNP:200702058
991 991 c, g dbSNP:1065740
994 994 c, t dbSNP:143455013
995 995 c, t dbSNP:1803623
996 996 c, t dbSNP:1062436
1005 1005 a, g dbSNP:371787931
1008 1008 c, t dbSNP:3203507
1012 1012 a, g dbSNP:750393514
1016 1016 a, c dbSNP:1062442
1019 1019 c, t dbSNP:758357246
1025 1025 c, g dbSNP:779733019
1031 1031 g, t dbSNP:751074589
1037 1037 c, t dbSNP:754701124
1040 1040 c, t dbSNP:746010467
1041 1041 g, t dbSNP:1803619
1051 1051 c, g dbSNP:568135688
1052 1052 c, t dbSNP:147970912
1053 1053 a, g dbSNP:771202486
1058 1058 c, g dbSNP:779190571
1064 1064 c, t dbSNP:200251380
1069 1069 c, t dbSNP:1803625
1072 1072 -, cc dbSNP:768116523
1073 1073 -, ccaccttc dbSNP:776091029
1073 1073 c, t dbSNP:1803621
1076 1076 c, t dbSNP:11549325
1078 1078 c, g dbSNP:772523425
1088 1088 c, g, t dbSNP:147124315
1090 1090 c, t dbSNP:768977954
1092 1092 g, t dbSNP:776716436
1094 1094 c, t dbSNP:199746238
1095 1095 c, g dbSNP:762031906
1097 1097 c, t dbSNP:138528853
1099 1099 a, g dbSNP:374950549
1100 1100 c, g dbSNP:763015743
1101 1101 a, g dbSNP:766389233
1109 1109 c, t dbSNP:751166351
1115 1115 g, t dbSNP:11549334
1118 1118 c, t dbSNP:754440369
1124 1124 c, t dbSNP:780684996
1130 1130 c, t dbSNP:748231452
1136 1136 c, t dbSNP:1109
1143 1143 a, g dbSNP:773557563
1148 1148 c, t dbSNP:545801389
1153 1153 a, t dbSNP:11549321
1158 1158 g, t dbSNP:770860006
1170 1170 a, g dbSNP:774143996
1172 1172 g, t dbSNP:3211600
1178 1178 a, c dbSNP:759418707
1179 1179 a, g dbSNP:767458626
1184 1184 c, t dbSNP:11549371
1191 1191 a, g dbSNP:774911921
1198 1198 -, c dbSNP:767752710
1199 1199 -, c dbSNP:759513625
1200 1200 c, t dbSNP:543637229
1201 1201 c, t dbSNP:763801346
1202 1202 c, t dbSNP:3211592
1203 1203 c, t dbSNP:748828754
1206 1206 a, g dbSNP:371417284
1210 1210 c, g, t dbSNP:11549320
1211 1211 c, t dbSNP:755481983
1219 1219 a, g dbSNP:376024489
1221 1221 a, g dbSNP:770280504
1236 1236 -, ag dbSNP:752810735
1238 1238 a, c dbSNP:781711772
1241 1241 c, g dbSNP:748236618
1242 1242 a, c dbSNP:756260656
1245 1245 -, cc dbSNP:756357878
1245 1245 a, c dbSNP:559712947
1246 1246 c, t dbSNP:373079410
1247 1247 c, t dbSNP:111385796
1253 1253 a, g dbSNP:138263904
1254 1254 c, t dbSNP:192090590
1289 1289 a, c dbSNP:561929037
1290 1290 c, t dbSNP:11549319
1306 1306 c, g dbSNP:550296309
1308 1308 a, c dbSNP:141846451
1313 1313 g, t dbSNP:1803622
1325 1325 c, t dbSNP:759172931
1332 1332 a, g dbSNP:3211598
1356 1356 a, c dbSNP:3211599
1363 1363 c, t dbSNP:760001399
1376 1376 g, t dbSNP:552128740
1377 1377 a, g dbSNP:565707202
1384 1384 c, g dbSNP:771754711
1397 1397 a, g, t dbSNP:112433978

Target ORF information:

RefSeq Version NM_002046
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_002046

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu20465D
Sequence Information ORF Nucleotide Sequence (Length: 882bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY000136.1, AF261085.1 and BE893087.1. On Jan 28, 2014 this sequence version replaced gi:378404907. Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 ##RefSeq-Attributes-END## ##Evidence-Data-START## CDS exon combination :: BC029340.1, BM478815.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTTACA TGTTCCAATA TGATTCCACC CATGGCAAAT TCCATGGCAC CGTCAAGGCT 
GAGAACGGGA AGCTTGTCAT CAATGGAAAT CCCATCACCA TCTTCCAGGA GCGAGATCCC
TCCAAAATCA AGTGGGGCGA TGCTGGCGCT GAGTACGTCG TGGAGTCCAC TGGCGTCTTC
ACCACCATGG AGAAGGCTGG GGCTCATTTG CAGGGGGGAG CCAAAAGGGT CATCATCTCT
GCCCCCTCTG CTGATGCCCC CATGTTCGTC ATGGGTGTGA ACCATGAGAA GTATGACAAC
AGCCTCAAGA TCATCAGCAA TGCCTCCTGC ACCACCAACT GCTTAGCACC CCTGGCCAAG
GTCATCCATG ACAACTTTGG TATCGTGGAA GGACTCATGA CCACAGTCCA TGCCATCACT
GCCACCCAGA AGACTGTGGA TGGCCCCTCC GGGAAACTGT GGCGTGATGG CCGCGGGGCT
CTCCAGAACA TCATCCCTGC CTCTACTGGC GCTGCCAAGG CTGTGGGCAA GGTCATCCCT
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTCC CCACTGCCAA CGTGTCAGTG
GTGGACCTGA CCTGCCGTCT AGAAAAACCT GCCAAATATG ATGACATCAA GAAGGTGGTG
AAGCAGGCGT CGGAGGGCCC CCTCAAGGGC ATCCTGGGCT ACACTGAGCA CCAGGTGGTC
TCCTCTGACT TCAACAGCGA CACCCACTCC TCCACCTTTG ACGCTGGGGC TGGCATTGCC
CTCAACGACC ACTTTGTCAA GCTCATTTCC TGGTATGACA ACGAATTTGG CTACAGCAAC
AGGGTGGTGG ACCTCATGGC CCACATGGCC TCCAAGGAGTT AA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001243728.1
CDS349..1230
Misc Feature(1)250..252(+)
Misc Feature(2)349..678(+)
Misc Feature(3)691..1164(+)
Exon (1)1..251
Gene:GAPDH
Gene Synonym:
Exon (2)252..351
Gene:GAPDH
Gene Synonym:
Exon (3)352..458
Gene:GAPDH
Gene Synonym:
Exon (4)459..549
Gene:GAPDH
Gene Synonym:
Exon (5)550..665
Gene:GAPDH
Gene Synonym:
Exon (6)666..747
Gene:GAPDH
Gene Synonym:
Exon (7)748..1160
Gene:GAPDH
Gene Synonym:
Exon (8)1161..1435
Gene:GAPDH
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:529459442
8 8 c, g dbSNP:549106552
15 15 c, t dbSNP:576918011
33 33 c, t dbSNP:761206408
50 50 c, g dbSNP:764891502
54 54 a, g dbSNP:373159375
60 60 c, t dbSNP:568847196
68 68 a, g dbSNP:750034352
82 82 g, t dbSNP:537878358
95 95 a, g dbSNP:766668880
129 129 a, c dbSNP:546089586
148 148 -, c dbSNP:138781672
187 187 a, g dbSNP:45534631
204 204 c, g dbSNP:45575233
210 210 c, t dbSNP:553603774
216 216 c, t dbSNP:573547578
217 217 a, t dbSNP:542448054
218 218 a, c dbSNP:770685632
237 237 c, t dbSNP:555864342
243 243 a, t dbSNP:758190990
258 258 c, t dbSNP:750965191
268 268 c, t dbSNP:758911385
282 282 a, g dbSNP:190547766
287 287 c, g dbSNP:45541435
297 297 c, t dbSNP:747064609
308 308 a, g dbSNP:11549361
310 310 a, g dbSNP:755187648
319 319 a, g dbSNP:376494973
324 324 a, t dbSNP:747986504
327 327 c, t dbSNP:769573085
328 328 c, g dbSNP:11549369
330 330 c, t dbSNP:140793249
333 333 c, t dbSNP:534209743
337 337 g, t dbSNP:11549328
339 339 c, t dbSNP:770803265
344 344 a, g dbSNP:773762261
345 345 c, g, t dbSNP:142283743
352 352 a, g dbSNP:759892796
359 359 c, t dbSNP:767648675
366 366 a, g dbSNP:752723540
372 372 c, t dbSNP:377360455
374 374 a, c dbSNP:200102749
381 381 c, t dbSNP:139204528
384 384 c, t dbSNP:527405745
386 386 a, g dbSNP:757057882
396 396 c, t dbSNP:778786251
398 398 c, t dbSNP:745827298
399 399 c, t dbSNP:771849987
400 400 a, g, t dbSNP:11549351
405 405 a, g dbSNP:746479173
411 411 g, t dbSNP:11549346
414 414 c, t dbSNP:201939440
415 415 a, g dbSNP:776400902
417 417 g, t dbSNP:11549355
424 424 c, g dbSNP:749674237
429 429 c, t dbSNP:771331311
430 430 a, c dbSNP:774521691
431 431 a, g dbSNP:201368443
437 437 a, g dbSNP:767870427
440 440 a, c dbSNP:775584055
450 450 c, t dbSNP:760719504
460 460 c, t dbSNP:755829002
468 468 a, c, g dbSNP:777266705
470 470 c, g, t dbSNP:772417505
479 479 a, g dbSNP:552376556
481 481 g, t dbSNP:113341953
486 486 c, t dbSNP:11549353
495 495 c, t dbSNP:200281741
504 504 c, t dbSNP:11549324
505 505 a, g dbSNP:371192512
507 507 c, t dbSNP:534300550
508 508 a, g dbSNP:770105201
518 518 c, t dbSNP:11549329
522 522 c, t dbSNP:371116598
523 523 a, g dbSNP:149945423
525 525 c, t dbSNP:11549337
528 528 c, t dbSNP:11549363
529 529 a, g dbSNP:751350310
531 531 a, c, t dbSNP:11266742
534 534 c, t dbSNP:759577147
537 537 c, g dbSNP:375963780
540 540 a, g dbSNP:11549374
546 546 c, t dbSNP:752299792
552 552 c, t dbSNP:11549350
555 555 a, t dbSNP:11549322
561 561 a, g dbSNP:760591074
564 564 a, g dbSNP:763523311
566 566 c, g, t dbSNP:11549340
567 567 a, g dbSNP:753299793
570 570 a, c, t dbSNP:11549349
579 579 c, t dbSNP:778509730
585 585 c, t dbSNP:751972977
591 591 c, t dbSNP:758897659
594 594 c, g, t dbSNP:368740142
596 596 c, t dbSNP:11549338
599 599 c, t dbSNP:146516445
600 600 c, t dbSNP:370350073
603 603 c, t dbSNP:1065691
606 606 a, c dbSNP:572265558
609 609 c, t dbSNP:771327842
615 615 c, t dbSNP:774700281
616 616 a, g dbSNP:745721511
618 618 c, t dbSNP:11549336
622 622 a, g dbSNP:771766319
624 624 c, t dbSNP:144761391
627 627 a, g dbSNP:760555162
634 634 a, g dbSNP:764055385
643 643 a, g dbSNP:74407849
648 648 c, t dbSNP:776162625
651 651 c, t dbSNP:761293659
656 656 a, g dbSNP:764928240
657 657 g, t dbSNP:372910824
658 658 a, g, t dbSNP:138863602
659 659 a, t dbSNP:767788286
660 660 c, t dbSNP:753064371
661 661 a, c dbSNP:376053895
669 669 c, t dbSNP:746840589
680 680 c, t dbSNP:11549333
686 686 a, g dbSNP:768495247
690 690 c, t dbSNP:780898155
703 703 g, t dbSNP:77154254
710 710 c, t dbSNP:748092877
716 716 a, g dbSNP:769671313
720 720 c, g dbSNP:772747939
723 723 c, t dbSNP:11549330
724 724 c, t dbSNP:11549326
725 725 g, t dbSNP:762501270
732 732 c, t dbSNP:770546388
733 733 a, g dbSNP:774047959
735 735 a, g dbSNP:562063348
741 741 a, t dbSNP:201481859
742 742 a, c dbSNP:201208495
744 744 c, t dbSNP:11549343
748 748 a, g dbSNP:766493985
760 760 g, t dbSNP:756189036
771 771 c, t dbSNP:369619209
783 783 -, tg dbSNP:747164357
786 786 a, g dbSNP:541601360
795 795 c, t dbSNP:554400197
798 798 c, t dbSNP:373252845
799 799 a, g dbSNP:754017958
801 801 g, t dbSNP:543121368
805 805 c, t dbSNP:376796183
806 806 -, tg dbSNP:778058544
818 818 c, g dbSNP:11549348
822 822 c, t dbSNP:368124080
826 826 c, g dbSNP:11549352
826 826 a, g dbSNP:749257900
829 829 c, t dbSNP:757090796
831 831 a, c, g dbSNP:372768955
834 834 a, g dbSNP:745337926
837 837 c, t dbSNP:771902302
838 838 a, g dbSNP:775271002
849 849 c, t dbSNP:370741285
850 850 c, t dbSNP:11549345
851 851 c, g dbSNP:771140596
855 855 a, t dbSNP:770291627
858 858 c, t dbSNP:773392187
866 866 a, g dbSNP:763539313
876 876 c, t dbSNP:766757138
878 878 a, g dbSNP:774531550
892 892 a, c dbSNP:759596917
897 897 c, t dbSNP:148843886
898 898 a, g dbSNP:752919766
918 918 c, t dbSNP:11549347
918 918 c, g dbSNP:756270449
930 930 a, c dbSNP:11549373
932 932 c, t dbSNP:763967581
933 933 c, t dbSNP:753533688
935 935 c, t dbSNP:757255996
936 936 c, t dbSNP:778691759
939 939 c, t dbSNP:191738457
949 949 g, t dbSNP:757981202
950 950 c, t dbSNP:779648965
964 964 c, t dbSNP:546169843
965 965 a, g dbSNP:768146299
970 970 -, aa dbSNP:749660368
972 972 a, g dbSNP:773658609
975 975 a, c dbSNP:1062429
981 981 c, g dbSNP:749586575
984 984 a, g dbSNP:776921270
986 986 -, atg dbSNP:779337992
986 986 -, a dbSNP:771432837
987 987 c, t dbSNP:771525480
988 988 g, t dbSNP:774833554
994 994 a, g dbSNP:759968940
1002 1002 -, ggt dbSNP:746549349
1005 1005 a, g dbSNP:559678529
1015 1015 a, g dbSNP:377461105
1016 1016 c, t dbSNP:528329572
1017 1017 a, g dbSNP:764189570
1019 1019 c, t dbSNP:11549316
1020 1020 a, g dbSNP:200702058
1025 1025 c, g dbSNP:1065740
1028 1028 c, t dbSNP:143455013
1029 1029 c, t dbSNP:1803623
1030 1030 c, t dbSNP:1062436
1039 1039 a, g dbSNP:371787931
1042 1042 c, t dbSNP:3203507
1046 1046 a, g dbSNP:750393514
1050 1050 a, c dbSNP:1062442
1053 1053 c, t dbSNP:758357246
1059 1059 c, g dbSNP:779733019
1065 1065 g, t dbSNP:751074589
1071 1071 c, t dbSNP:754701124
1074 1074 c, t dbSNP:746010467
1075 1075 g, t dbSNP:1803619
1085 1085 c, g dbSNP:568135688
1086 1086 c, t dbSNP:147970912
1087 1087 a, g dbSNP:771202486
1092 1092 c, g dbSNP:779190571
1098 1098 c, t dbSNP:200251380
1103 1103 c, t dbSNP:1803625
1106 1106 -, cc dbSNP:768116523
1107 1107 -, ccaccttc dbSNP:776091029
1107 1107 c, t dbSNP:1803621
1110 1110 c, t dbSNP:11549325
1112 1112 c, g dbSNP:772523425
1122 1122 c, g, t dbSNP:147124315
1124 1124 c, t dbSNP:768977954
1126 1126 g, t dbSNP:776716436
1128 1128 c, t dbSNP:199746238
1129 1129 c, g dbSNP:762031906
1131 1131 c, t dbSNP:138528853
1133 1133 a, g dbSNP:374950549
1134 1134 c, g dbSNP:763015743
1135 1135 a, g dbSNP:766389233
1143 1143 c, t dbSNP:751166351
1149 1149 g, t dbSNP:11549334
1152 1152 c, t dbSNP:754440369
1158 1158 c, t dbSNP:780684996
1164 1164 c, t dbSNP:748231452
1170 1170 c, t dbSNP:1109
1177 1177 a, g dbSNP:773557563
1182 1182 c, t dbSNP:545801389
1187 1187 a, t dbSNP:11549321
1192 1192 g, t dbSNP:770860006
1204 1204 a, g dbSNP:774143996
1206 1206 g, t dbSNP:3211600
1212 1212 a, c dbSNP:759418707
1213 1213 a, g dbSNP:767458626
1218 1218 c, t dbSNP:11549371
1225 1225 a, g dbSNP:774911921
1232 1232 -, c dbSNP:767752710
1233 1233 -, c dbSNP:759513625
1234 1234 c, t dbSNP:543637229
1235 1235 c, t dbSNP:763801346
1236 1236 c, t dbSNP:3211592
1237 1237 c, t dbSNP:748828754
1240 1240 a, g dbSNP:371417284
1244 1244 c, g, t dbSNP:11549320
1245 1245 c, t dbSNP:755481983
1253 1253 a, g dbSNP:376024489
1255 1255 a, g dbSNP:770280504
1270 1270 -, ag dbSNP:752810735
1272 1272 a, c dbSNP:781711772
1275 1275 c, g dbSNP:748236618
1276 1276 a, c dbSNP:756260656
1279 1279 -, cc dbSNP:756357878
1279 1279 a, c dbSNP:559712947
1280 1280 c, t dbSNP:373079410
1281 1281 c, t dbSNP:111385796
1287 1287 a, g dbSNP:138263904
1288 1288 c, t dbSNP:192090590
1323 1323 a, c dbSNP:561929037
1324 1324 c, t dbSNP:11549319
1340 1340 c, g dbSNP:550296309
1342 1342 a, c dbSNP:141846451
1347 1347 g, t dbSNP:1803622
1359 1359 c, t dbSNP:759172931
1366 1366 a, g dbSNP:3211598
1390 1390 a, c dbSNP:3211599
1397 1397 c, t dbSNP:760001399
1410 1410 g, t dbSNP:552128740
1411 1411 a, g dbSNP:565707202
1418 1418 c, g dbSNP:771754711
1431 1431 a, g, t dbSNP:112433978

Target ORF information:

RefSeq Version NM_001256799
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001256799

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTTACA TGTTCCAATA TGATTCCACC CATGGCAAAT TCCATGGCAC CGTCAAGGCT 
GAGAACGGGA AGCTTGTCAT CAATGGAAAT CCCATCACCA TCTTCCAGGA GCGAGATCCC
TCCAAAATCA AGTGGGGCGA TGCTGGCGCT GAGTACGTCG TGGAGTCCAC TGGCGTCTTC
ACCACCATGG AGAAGGCTGG GGCTCATTTG CAGGGGGGAG CCAAAAGGGT CATCATCTCT
GCCCCCTCTG CTGATGCCCC CATGTTCGTC ATGGGTGTGA ACCATGAGAA GTATGACAAC
AGCCTCAAGA TCATCAGCAA TGCCTCCTGC ACCACCAACT GCTTAGCACC CCTGGCCAAG
GTCATCCATG ACAACTTTGG TATCGTGGAA GGACTCATGA CCACAGTCCA TGCCATCACT
GCCACCCAGA AGACTGTGGA TGGCCCCTCC GGGAAACTGT GGCGTGATGG CCGCGGGGCT
CTCCAGAACA TCATCCCTGC CTCTACTGGC GCTGCCAAGG CTGTGGGCAA GGTCATCCCT
GAGCTGAACG GGAAGCTCAC TGGCATGGCC TTCCGTGTCC CCACTGCCAA CGTGTCAGTG
GTGGACCTGA CCTGCCGTCT AGAAAAACCT GCCAAATATG ATGACATCAA GAAGGTGGTG
AAGCAGGCGT CGGAGGGCCC CCTCAAGGGC ATCCTGGGCT ACACTGAGCA CCAGGTGGTC
TCCTCTGACT TCAACAGCGA CACCCACTCC TCCACCTTTG ACGCTGGGGC TGGCATTGCC
CTCAACGACC ACTTTGTCAA GCTCATTTCC TGGTATGACA ACGAATTTGG CTACAGCAAC
AGGGTGGTGG ACCTCATGGC CCACATGGCC TCCAAGGAGTT AA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu20566D
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY004110.1, HY022295.1, BC023632.2 and BE893087.1. Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023632.2, X01677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 Protein has antimicrobial activity :: PMID: 22832495 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001276675.1
CDS175..1182
Misc Feature(1)121..123(+)
Misc Feature(2)178..618(+)
Misc Feature(3)181..1179(+)
Misc Feature(4)184..630(+)
Misc Feature(5)187..189(+)
Misc Feature(6)199..201(+)
Misc Feature(7)298..300(+)
Misc Feature(8)355..357(+)
Misc Feature(9)364..366(+)
Misc Feature(10)370..372(+)
Misc Feature(11)382..384(+)
Misc Feature(12)397..399(+)
Misc Feature(13)421..423(+)
Misc Feature(14)538..540(+)
Misc Feature(15)616..618(+)
Misc Feature(16)619..621(+)
Misc Feature(17)625..633(+)
Misc Feature(18)625..627(+)
Misc Feature(19)628..630(+)
Misc Feature(20)628..630(+)
Misc Feature(21)628..630(+)
Misc Feature(22)637..639(+)
Misc Feature(23)643..1116(+)
Misc Feature(24)703..705(+)
Misc Feature(25)709..711(+)
Misc Feature(26)718..720(+)
Misc Feature(27)724..726(+)
Misc Feature(28)754..756(+)
Misc Feature(29)754..756(+)
Misc Feature(30)754..756(+)
Misc Feature(31)805..810(+)
Misc Feature(32)805..807(+)
Misc Feature(33)817..819(+)
Misc Feature(34)817..819(+)
Misc Feature(35)829..831(+)
Misc Feature(36)847..849(+)
Misc Feature(37)853..855(+)
Misc Feature(38)853..855(+)
Misc Feature(39)859..861(+)
Misc Feature(40)883..885(+)
Misc Feature(41)895..897(+)
Misc Feature(42)907..924(+)
Misc Feature(43)913..915(+)
Misc Feature(44)934..936(+)
Misc Feature(45)952..954(+)
Misc Feature(46)961..963(+)
Misc Feature(47)1108..1110(+)
Misc Feature(48)1120..1122(+)
Misc Feature(49)1174..1176(+)
Exon (1)1..203
Gene:GAPDH
Gene Synonym:
Exon (2)204..303
Gene:GAPDH
Gene Synonym:
Exon (3)304..410
Gene:GAPDH
Gene Synonym:
Exon (4)411..501
Gene:GAPDH
Gene Synonym:
Exon (5)502..617
Gene:GAPDH
Gene Synonym:
Exon (6)618..699
Gene:GAPDH
Gene Synonym:
Exon (7)700..1112
Gene:GAPDH
Gene Synonym:
Exon (8)1113..1387
Gene:GAPDH
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, g dbSNP:540034703
20 20 a, t dbSNP:371892266
27 27 g, t dbSNP:188796702
46 46 c, t dbSNP:553144566
56 56 c, t dbSNP:45497495
74 74 a, g dbSNP:370224073
75 75 c, t dbSNP:555362742
82 82 g, t dbSNP:575095159
90 90 g, t dbSNP:544502915
103 103 g, t dbSNP:3741915
124 124 c, g, t dbSNP:752478899
126 126 a, g dbSNP:577459586
127 127 a, c dbSNP:753432264
128 128 a, c, t dbSNP:368616050
131 131 c, t dbSNP:750074039
136 136 a, c, g, t dbSNP:370942649
137 137 c, g, t dbSNP:559451450
140 140 c, t dbSNP:200278792
142 142 -, ctccgcg dbSNP:780336407
144 144 c, t dbSNP:376312703
145 145 c, t dbSNP:749442640
147 147 c, t dbSNP:770909874
149 149 c, t dbSNP:774503837
152 152 c, t dbSNP:759849225
154 154 c, g dbSNP:772368359
156 156 a, g, t dbSNP:35318117
158 158 c, t dbSNP:372129246
161 161 a, g dbSNP:764146133
163 163 c, t dbSNP:753709203
167 167 c, g dbSNP:1136666
171 171 c, t dbSNP:764733553
176 176 c, t dbSNP:147280944
178 178 a, g dbSNP:758070479
184 184 a, g dbSNP:367765818
185 185 c, t dbSNP:369545182
189 189 c, g dbSNP:371408635
190 190 a, g dbSNP:778410218
194 194 c, g dbSNP:749762041
195 195 a, c dbSNP:771409071
201 201 c, t dbSNP:530766121
210 210 c, t dbSNP:750965191
220 220 c, t dbSNP:758911385
234 234 a, g dbSNP:190547766
239 239 c, g dbSNP:45541435
249 249 c, t dbSNP:747064609
260 260 a, g dbSNP:11549361
262 262 a, g dbSNP:755187648
271 271 a, g dbSNP:376494973
276 276 a, t dbSNP:747986504
279 279 c, t dbSNP:769573085
280 280 c, g dbSNP:11549369
282 282 c, t dbSNP:140793249
285 285 c, t dbSNP:534209743
289 289 g, t dbSNP:11549328
291 291 c, t dbSNP:770803265
296 296 a, g dbSNP:773762261
297 297 c, g, t dbSNP:142283743
304 304 a, g dbSNP:759892796
311 311 c, t dbSNP:767648675
318 318 a, g dbSNP:752723540
324 324 c, t dbSNP:377360455
326 326 a, c dbSNP:200102749
333 333 c, t dbSNP:139204528
336 336 c, t dbSNP:527405745
338 338 a, g dbSNP:757057882
348 348 c, t dbSNP:778786251
350 350 c, t dbSNP:745827298
351 351 c, t dbSNP:771849987
352 352 a, g, t dbSNP:11549351
357 357 a, g dbSNP:746479173
363 363 g, t dbSNP:11549346
366 366 c, t dbSNP:201939440
367 367 a, g dbSNP:776400902
369 369 g, t dbSNP:11549355
376 376 c, g dbSNP:749674237
381 381 c, t dbSNP:771331311
382 382 a, c dbSNP:774521691
383 383 a, g dbSNP:201368443
389 389 a, g dbSNP:767870427
392 392 a, c dbSNP:775584055
402 402 c, t dbSNP:760719504
412 412 c, t dbSNP:755829002
420 420 a, c, g dbSNP:777266705
422 422 c, g, t dbSNP:772417505
431 431 a, g dbSNP:552376556
433 433 g, t dbSNP:113341953
438 438 c, t dbSNP:11549353
447 447 c, t dbSNP:200281741
456 456 c, t dbSNP:11549324
457 457 a, g dbSNP:371192512
459 459 c, t dbSNP:534300550
460 460 a, g dbSNP:770105201
470 470 c, t dbSNP:11549329
474 474 c, t dbSNP:371116598
475 475 a, g dbSNP:149945423
477 477 c, t dbSNP:11549337
480 480 c, t dbSNP:11549363
481 481 a, g dbSNP:751350310
483 483 a, c, t dbSNP:11266742
486 486 c, t dbSNP:759577147
489 489 c, g dbSNP:375963780
492 492 a, g dbSNP:11549374
498 498 c, t dbSNP:752299792
504 504 c, t dbSNP:11549350
507 507 a, t dbSNP:11549322
513 513 a, g dbSNP:760591074
516 516 a, g dbSNP:763523311
518 518 c, g, t dbSNP:11549340
519 519 a, g dbSNP:753299793
522 522 a, c, t dbSNP:11549349
531 531 c, t dbSNP:778509730
537 537 c, t dbSNP:751972977
543 543 c, t dbSNP:758897659
546 546 c, g, t dbSNP:368740142
548 548 c, t dbSNP:11549338
551 551 c, t dbSNP:146516445
552 552 c, t dbSNP:370350073
555 555 c, t dbSNP:1065691
558 558 a, c dbSNP:572265558
561 561 c, t dbSNP:771327842
567 567 c, t dbSNP:774700281
568 568 a, g dbSNP:745721511
570 570 c, t dbSNP:11549336
574 574 a, g dbSNP:771766319
576 576 c, t dbSNP:144761391
579 579 a, g dbSNP:760555162
586 586 a, g dbSNP:764055385
595 595 a, g dbSNP:74407849
600 600 c, t dbSNP:776162625
603 603 c, t dbSNP:761293659
608 608 a, g dbSNP:764928240
609 609 g, t dbSNP:372910824
610 610 a, g, t dbSNP:138863602
611 611 a, t dbSNP:767788286
612 612 c, t dbSNP:753064371
613 613 a, c dbSNP:376053895
621 621 c, t dbSNP:746840589
632 632 c, t dbSNP:11549333
638 638 a, g dbSNP:768495247
642 642 c, t dbSNP:780898155
655 655 g, t dbSNP:77154254
662 662 c, t dbSNP:748092877
668 668 a, g dbSNP:769671313
672 672 c, g dbSNP:772747939
675 675 c, t dbSNP:11549330
676 676 c, t dbSNP:11549326
677 677 g, t dbSNP:762501270
684 684 c, t dbSNP:770546388
685 685 a, g dbSNP:774047959
687 687 a, g dbSNP:562063348
693 693 a, t dbSNP:201481859
694 694 a, c dbSNP:201208495
696 696 c, t dbSNP:11549343
700 700 a, g dbSNP:766493985
712 712 g, t dbSNP:756189036
723 723 c, t dbSNP:369619209
735 735 -, tg dbSNP:747164357
738 738 a, g dbSNP:541601360
747 747 c, t dbSNP:554400197
750 750 c, t dbSNP:373252845
751 751 a, g dbSNP:754017958
753 753 g, t dbSNP:543121368
757 757 c, t dbSNP:376796183
758 758 -, tg dbSNP:778058544
770 770 c, g dbSNP:11549348
774 774 c, t dbSNP:368124080
778 778 c, g dbSNP:11549352
778 778 a, g dbSNP:749257900
781 781 c, t dbSNP:757090796
783 783 a, c, g dbSNP:372768955
786 786 a, g dbSNP:745337926
789 789 c, t dbSNP:771902302
790 790 a, g dbSNP:775271002
801 801 c, t dbSNP:370741285
802 802 c, t dbSNP:11549345
803 803 c, g dbSNP:771140596
807 807 a, t dbSNP:770291627
810 810 c, t dbSNP:773392187
818 818 a, g dbSNP:763539313
828 828 c, t dbSNP:766757138
830 830 a, g dbSNP:774531550
844 844 a, c dbSNP:759596917
849 849 c, t dbSNP:148843886
850 850 a, g dbSNP:752919766
870 870 c, t dbSNP:11549347
870 870 c, g dbSNP:756270449
882 882 a, c dbSNP:11549373
884 884 c, t dbSNP:763967581
885 885 c, t dbSNP:753533688
887 887 c, t dbSNP:757255996
888 888 c, t dbSNP:778691759
891 891 c, t dbSNP:191738457
901 901 g, t dbSNP:757981202
902 902 c, t dbSNP:779648965
916 916 c, t dbSNP:546169843
917 917 a, g dbSNP:768146299
922 922 -, aa dbSNP:749660368
924 924 a, g dbSNP:773658609
927 927 a, c dbSNP:1062429
933 933 c, g dbSNP:749586575
936 936 a, g dbSNP:776921270
938 938 -, atg dbSNP:779337992
938 938 -, a dbSNP:771432837
939 939 c, t dbSNP:771525480
940 940 g, t dbSNP:774833554
946 946 a, g dbSNP:759968940
954 954 -, ggt dbSNP:746549349
957 957 a, g dbSNP:559678529
967 967 a, g dbSNP:377461105
968 968 c, t dbSNP:528329572
969 969 a, g dbSNP:764189570
971 971 c, t dbSNP:11549316
972 972 a, g dbSNP:200702058
977 977 c, g dbSNP:1065740
980 980 c, t dbSNP:143455013
981 981 c, t dbSNP:1803623
982 982 c, t dbSNP:1062436
991 991 a, g dbSNP:371787931
994 994 c, t dbSNP:3203507
998 998 a, g dbSNP:750393514
1002 1002 a, c dbSNP:1062442
1005 1005 c, t dbSNP:758357246
1011 1011 c, g dbSNP:779733019
1017 1017 g, t dbSNP:751074589
1023 1023 c, t dbSNP:754701124
1026 1026 c, t dbSNP:746010467
1027 1027 g, t dbSNP:1803619
1037 1037 c, g dbSNP:568135688
1038 1038 c, t dbSNP:147970912
1039 1039 a, g dbSNP:771202486
1044 1044 c, g dbSNP:779190571
1050 1050 c, t dbSNP:200251380
1055 1055 c, t dbSNP:1803625
1058 1058 -, cc dbSNP:768116523
1059 1059 -, ccaccttc dbSNP:776091029
1059 1059 c, t dbSNP:1803621
1062 1062 c, t dbSNP:11549325
1064 1064 c, g dbSNP:772523425
1074 1074 c, g, t dbSNP:147124315
1076 1076 c, t dbSNP:768977954
1078 1078 g, t dbSNP:776716436
1080 1080 c, t dbSNP:199746238
1081 1081 c, g dbSNP:762031906
1083 1083 c, t dbSNP:138528853
1085 1085 a, g dbSNP:374950549
1086 1086 c, g dbSNP:763015743
1087 1087 a, g dbSNP:766389233
1095 1095 c, t dbSNP:751166351
1101 1101 g, t dbSNP:11549334
1104 1104 c, t dbSNP:754440369
1110 1110 c, t dbSNP:780684996
1116 1116 c, t dbSNP:748231452
1122 1122 c, t dbSNP:1109
1129 1129 a, g dbSNP:773557563
1134 1134 c, t dbSNP:545801389
1139 1139 a, t dbSNP:11549321
1144 1144 g, t dbSNP:770860006
1156 1156 a, g dbSNP:774143996
1158 1158 g, t dbSNP:3211600
1164 1164 a, c dbSNP:759418707
1165 1165 a, g dbSNP:767458626
1170 1170 c, t dbSNP:11549371
1177 1177 a, g dbSNP:774911921
1184 1184 -, c dbSNP:767752710
1185 1185 -, c dbSNP:759513625
1186 1186 c, t dbSNP:543637229
1187 1187 c, t dbSNP:763801346
1188 1188 c, t dbSNP:3211592
1189 1189 c, t dbSNP:748828754
1192 1192 a, g dbSNP:371417284
1196 1196 c, g, t dbSNP:11549320
1197 1197 c, t dbSNP:755481983
1205 1205 a, g dbSNP:376024489
1207 1207 a, g dbSNP:770280504
1222 1222 -, ag dbSNP:752810735
1224 1224 a, c dbSNP:781711772
1227 1227 c, g dbSNP:748236618
1228 1228 a, c dbSNP:756260656
1231 1231 -, cc dbSNP:756357878
1231 1231 a, c dbSNP:559712947
1232 1232 c, t dbSNP:373079410
1233 1233 c, t dbSNP:111385796
1239 1239 a, g dbSNP:138263904
1240 1240 c, t dbSNP:192090590
1275 1275 a, c dbSNP:561929037
1276 1276 c, t dbSNP:11549319
1292 1292 c, g dbSNP:550296309
1294 1294 a, c dbSNP:141846451
1299 1299 g, t dbSNP:1803622
1311 1311 c, t dbSNP:759172931
1318 1318 a, g dbSNP:3211598
1342 1342 a, c dbSNP:3211599
1349 1349 c, t dbSNP:760001399
1362 1362 g, t dbSNP:552128740
1363 1363 a, g dbSNP:565707202
1370 1370 c, g dbSNP:771754711
1383 1383 a, g, t dbSNP:112433978

Target ORF information:

RefSeq Version NM_001289746
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001289746

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu20566D
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product glyceraldehyde-3-phosphate dehydrogenase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY046784.1, BM763361.1, M33197.1 and BE893087.1. Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC004109.2, BC023632.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 20727968 Protein has antimicrobial activity :: PMID: 22832495 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001276674.1
CDS281..1288
Misc Feature(1)197..199(+)
Misc Feature(2)284..724(+)
Misc Feature(3)287..1285(+)
Misc Feature(4)290..736(+)
Misc Feature(5)293..295(+)
Misc Feature(6)305..307(+)
Misc Feature(7)404..406(+)
Misc Feature(8)461..463(+)
Misc Feature(9)470..472(+)
Misc Feature(10)476..478(+)
Misc Feature(11)488..490(+)
Misc Feature(12)503..505(+)
Misc Feature(13)527..529(+)
Misc Feature(14)644..646(+)
Misc Feature(15)722..724(+)
Misc Feature(16)725..727(+)
Misc Feature(17)731..739(+)
Misc Feature(18)731..733(+)
Misc Feature(19)734..736(+)
Misc Feature(20)734..736(+)
Misc Feature(21)734..736(+)
Misc Feature(22)743..745(+)
Misc Feature(23)749..1222(+)
Misc Feature(24)809..811(+)
Misc Feature(25)815..817(+)
Misc Feature(26)824..826(+)
Misc Feature(27)830..832(+)
Misc Feature(28)860..862(+)
Misc Feature(29)860..862(+)
Misc Feature(30)860..862(+)
Misc Feature(31)911..916(+)
Misc Feature(32)911..913(+)
Misc Feature(33)923..925(+)
Misc Feature(34)923..925(+)
Misc Feature(35)935..937(+)
Misc Feature(36)953..955(+)
Misc Feature(37)959..961(+)
Misc Feature(38)959..961(+)
Misc Feature(39)965..967(+)
Misc Feature(40)989..991(+)
Misc Feature(41)1001..1003(+)
Misc Feature(42)1013..1030(+)
Misc Feature(43)1019..1021(+)
Misc Feature(44)1040..1042(+)
Misc Feature(45)1058..1060(+)
Misc Feature(46)1067..1069(+)
Misc Feature(47)1214..1216(+)
Misc Feature(48)1226..1228(+)
Misc Feature(49)1280..1282(+)
Exon (1)1..257
Gene:GAPDH
Gene Synonym:
Exon (2)258..309
Gene:GAPDH
Gene Synonym:
Exon (3)310..409
Gene:GAPDH
Gene Synonym:
Exon (4)410..516
Gene:GAPDH
Gene Synonym:
Exon (5)517..607
Gene:GAPDH
Gene Synonym:
Exon (6)608..723
Gene:GAPDH
Gene Synonym:
Exon (7)724..805
Gene:GAPDH
Gene Synonym:
Exon (8)806..1218
Gene:GAPDH
Gene Synonym:
Exon (9)1219..1493
Gene:GAPDH
Gene Synonym:
Translation
Position Chain Variation Link
33 33 c, g dbSNP:7307229
40 40 c, g dbSNP:112427350
41 41 -, g dbSNP:71450157
46 46 c, g dbSNP:780164757
49 49 c, g, t dbSNP:111720901
69 69 c, t dbSNP:45561238
107 107 c, t dbSNP:45571036
109 109 c, t dbSNP:45508900
114 114 c, t dbSNP:564320185
116 116 c, t dbSNP:532940876
133 133 a, g dbSNP:45568532
136 136 a, g dbSNP:559414721
142 142 c, t dbSNP:528502920
143 143 c, t dbSNP:367946267
145 145 c, t dbSNP:527918526
148 148 c, t dbSNP:551180067
150 150 c, t dbSNP:3199979
151 151 c, t dbSNP:11549332
157 157 c, t dbSNP:537766086
203 203 c, g dbSNP:184407116
215 215 a, g dbSNP:571028238
258 258 c, t dbSNP:759849225
260 260 c, g dbSNP:772368359
262 262 a, g, t dbSNP:35318117
264 264 c, t dbSNP:372129246
267 267 a, g dbSNP:764146133
269 269 c, t dbSNP:753709203
273 273 c, g dbSNP:1136666
277 277 c, t dbSNP:764733553
282 282 c, t dbSNP:147280944
284 284 a, g dbSNP:758070479
290 290 a, g dbSNP:367765818
291 291 c, t dbSNP:369545182
295 295 c, g dbSNP:371408635
296 296 a, g dbSNP:778410218
300 300 c, g dbSNP:749762041
301 301 a, c dbSNP:771409071
307 307 c, t dbSNP:530766121
316 316 c, t dbSNP:750965191
326 326 c, t dbSNP:758911385
340 340 a, g dbSNP:190547766
345 345 c, g dbSNP:45541435
355 355 c, t dbSNP:747064609
366 366 a, g dbSNP:11549361
368 368 a, g dbSNP:755187648
377 377 a, g dbSNP:376494973
382 382 a, t dbSNP:747986504
385 385 c, t dbSNP:769573085
386 386 c, g dbSNP:11549369
388 388 c, t dbSNP:140793249
391 391 c, t dbSNP:534209743
395 395 g, t dbSNP:11549328
397 397 c, t dbSNP:770803265
402 402 a, g dbSNP:773762261
403 403 c, g, t dbSNP:142283743
410 410 a, g dbSNP:759892796
417 417 c, t dbSNP:767648675
424 424 a, g dbSNP:752723540
430 430 c, t dbSNP:377360455
432 432 a, c dbSNP:200102749
439 439 c, t dbSNP:139204528
442 442 c, t dbSNP:527405745
444 444 a, g dbSNP:757057882
454 454 c, t dbSNP:778786251
456 456 c, t dbSNP:745827298
457 457 c, t dbSNP:771849987
458 458 a, g, t dbSNP:11549351
463 463 a, g dbSNP:746479173
469 469 g, t dbSNP:11549346
472 472 c, t dbSNP:201939440
473 473 a, g dbSNP:776400902
475 475 g, t dbSNP:11549355
482 482 c, g dbSNP:749674237
487 487 c, t dbSNP:771331311
488 488 a, c dbSNP:774521691
489 489 a, g dbSNP:201368443
495 495 a, g dbSNP:767870427
498 498 a, c dbSNP:775584055
508 508 c, t dbSNP:760719504
518 518 c, t dbSNP:755829002
526 526 a, c, g dbSNP:777266705
528 528 c, g, t dbSNP:772417505
537 537 a, g dbSNP:552376556
539 539 g, t dbSNP:113341953
544 544 c, t dbSNP:11549353
553 553 c, t dbSNP:200281741
562 562 c, t dbSNP:11549324
563 563 a, g dbSNP:371192512
565 565 c, t dbSNP:534300550
566 566 a, g dbSNP:770105201
576 576 c, t dbSNP:11549329
580 580 c, t dbSNP:371116598
581 581 a, g dbSNP:149945423
583 583 c, t dbSNP:11549337
586 586 c, t dbSNP:11549363
587 587 a, g dbSNP:751350310
589 589 a, c, t dbSNP:11266742
592 592 c, t dbSNP:759577147
595 595 c, g dbSNP:375963780
598 598 a, g dbSNP:11549374
604 604 c, t dbSNP:752299792
610 610 c, t dbSNP:11549350
613 613 a, t dbSNP:11549322
619 619 a, g dbSNP:760591074
622 622 a, g dbSNP:763523311
624 624 c, g, t dbSNP:11549340
625 625 a, g dbSNP:753299793
628 628 a, c, t dbSNP:11549349
637 637 c, t dbSNP:778509730
643 643 c, t dbSNP:751972977
649 649 c, t dbSNP:758897659
652 652 c, g, t dbSNP:368740142
654 654 c, t dbSNP:11549338
657 657 c, t dbSNP:146516445
658 658 c, t dbSNP:370350073
661 661 c, t dbSNP:1065691
664 664 a, c dbSNP:572265558
667 667 c, t dbSNP:771327842
673 673 c, t dbSNP:774700281
674 674 a, g dbSNP:745721511
676 676 c, t dbSNP:11549336
680 680 a, g dbSNP:771766319
682 682 c, t dbSNP:144761391
685 685 a, g dbSNP:760555162
692 692 a, g dbSNP:764055385
701 701 a, g dbSNP:74407849
706 706 c, t dbSNP:776162625
709 709 c, t dbSNP:761293659
714 714 a, g dbSNP:764928240
715 715 g, t dbSNP:372910824
716 716 a, g, t dbSNP:138863602
717 717 a, t dbSNP:767788286
718 718 c, t dbSNP:753064371
719 719 a, c dbSNP:376053895
727 727 c, t dbSNP:746840589
738 738 c, t dbSNP:11549333
744 744 a, g dbSNP:768495247
748 748 c, t dbSNP:780898155
761 761 g, t dbSNP:77154254
768 768 c, t dbSNP:748092877
774 774 a, g dbSNP:769671313
778 778 c, g dbSNP:772747939
781 781 c, t dbSNP:11549330
782 782 c, t dbSNP:11549326
783 783 g, t dbSNP:762501270
790 790 c, t dbSNP:770546388
791 791 a, g dbSNP:774047959
793 793 a, g dbSNP:562063348
799 799 a, t dbSNP:201481859
800 800 a, c dbSNP:201208495
802 802 c, t dbSNP:11549343
806 806 a, g dbSNP:766493985
818 818 g, t dbSNP:756189036
829 829 c, t dbSNP:369619209
841 841 -, tg dbSNP:747164357
844 844 a, g dbSNP:541601360
853 853 c, t dbSNP:554400197
856 856 c, t dbSNP:373252845
857 857 a, g dbSNP:754017958
859 859 g, t dbSNP:543121368
863 863 c, t dbSNP:376796183
864 864 -, tg dbSNP:778058544
876 876 c, g dbSNP:11549348
880 880 c, t dbSNP:368124080
884 884 c, g dbSNP:11549352
884 884 a, g dbSNP:749257900
887 887 c, t dbSNP:757090796
889 889 a, c, g dbSNP:372768955
892 892 a, g dbSNP:745337926
895 895 c, t dbSNP:771902302
896 896 a, g dbSNP:775271002
907 907 c, t dbSNP:370741285
908 908 c, t dbSNP:11549345
909 909 c, g dbSNP:771140596
913 913 a, t dbSNP:770291627
916 916 c, t dbSNP:773392187
924 924 a, g dbSNP:763539313
934 934 c, t dbSNP:766757138
936 936 a, g dbSNP:774531550
950 950 a, c dbSNP:759596917
955 955 c, t dbSNP:148843886
956 956 a, g dbSNP:752919766
976 976 c, t dbSNP:11549347
976 976 c, g dbSNP:756270449
988 988 a, c dbSNP:11549373
990 990 c, t dbSNP:763967581
991 991 c, t dbSNP:753533688
993 993 c, t dbSNP:757255996
994 994 c, t dbSNP:778691759
997 997 c, t dbSNP:191738457
1007 1007 g, t dbSNP:757981202
1008 1008 c, t dbSNP:779648965
1022 1022 c, t dbSNP:546169843
1023 1023 a, g dbSNP:768146299
1028 1028 -, aa dbSNP:749660368
1030 1030 a, g dbSNP:773658609
1033 1033 a, c dbSNP:1062429
1039 1039 c, g dbSNP:749586575
1042 1042 a, g dbSNP:776921270
1044 1044 -, atg dbSNP:779337992
1044 1044 -, a dbSNP:771432837
1045 1045 c, t dbSNP:771525480
1046 1046 g, t dbSNP:774833554
1052 1052 a, g dbSNP:759968940
1060 1060 -, ggt dbSNP:746549349
1063 1063 a, g dbSNP:559678529
1073 1073 a, g dbSNP:377461105
1074 1074 c, t dbSNP:528329572
1075 1075 a, g dbSNP:764189570
1077 1077 c, t dbSNP:11549316
1078 1078 a, g dbSNP:200702058
1083 1083 c, g dbSNP:1065740
1086 1086 c, t dbSNP:143455013
1087 1087 c, t dbSNP:1803623
1088 1088 c, t dbSNP:1062436
1097 1097 a, g dbSNP:371787931
1100 1100 c, t dbSNP:3203507
1104 1104 a, g dbSNP:750393514
1108 1108 a, c dbSNP:1062442
1111 1111 c, t dbSNP:758357246
1117 1117 c, g dbSNP:779733019
1123 1123 g, t dbSNP:751074589
1129 1129 c, t dbSNP:754701124
1132 1132 c, t dbSNP:746010467
1133 1133 g, t dbSNP:1803619
1143 1143 c, g dbSNP:568135688
1144 1144 c, t dbSNP:147970912
1145 1145 a, g dbSNP:771202486
1150 1150 c, g dbSNP:779190571
1156 1156 c, t dbSNP:200251380
1161 1161 c, t dbSNP:1803625
1164 1164 -, cc dbSNP:768116523
1165 1165 -, ccaccttc dbSNP:776091029
1165 1165 c, t dbSNP:1803621
1168 1168 c, t dbSNP:11549325
1170 1170 c, g dbSNP:772523425
1180 1180 c, g, t dbSNP:147124315
1182 1182 c, t dbSNP:768977954
1184 1184 g, t dbSNP:776716436
1186 1186 c, t dbSNP:199746238
1187 1187 c, g dbSNP:762031906
1189 1189 c, t dbSNP:138528853
1191 1191 a, g dbSNP:374950549
1192 1192 c, g dbSNP:763015743
1193 1193 a, g dbSNP:766389233
1201 1201 c, t dbSNP:751166351
1207 1207 g, t dbSNP:11549334
1210 1210 c, t dbSNP:754440369
1216 1216 c, t dbSNP:780684996
1222 1222 c, t dbSNP:748231452
1228 1228 c, t dbSNP:1109
1235 1235 a, g dbSNP:773557563
1240 1240 c, t dbSNP:545801389
1245 1245 a, t dbSNP:11549321
1250 1250 g, t dbSNP:770860006
1262 1262 a, g dbSNP:774143996
1264 1264 g, t dbSNP:3211600
1270 1270 a, c dbSNP:759418707
1271 1271 a, g dbSNP:767458626
1276 1276 c, t dbSNP:11549371
1283 1283 a, g dbSNP:774911921
1290 1290 -, c dbSNP:767752710
1291 1291 -, c dbSNP:759513625
1292 1292 c, t dbSNP:543637229
1293 1293 c, t dbSNP:763801346
1294 1294 c, t dbSNP:3211592
1295 1295 c, t dbSNP:748828754
1298 1298 a, g dbSNP:371417284
1302 1302 c, g, t dbSNP:11549320
1303 1303 c, t dbSNP:755481983
1311 1311 a, g dbSNP:376024489
1313 1313 a, g dbSNP:770280504
1328 1328 -, ag dbSNP:752810735
1330 1330 a, c dbSNP:781711772
1333 1333 c, g dbSNP:748236618
1334 1334 a, c dbSNP:756260656
1337 1337 -, cc dbSNP:756357878
1337 1337 a, c dbSNP:559712947
1338 1338 c, t dbSNP:373079410
1339 1339 c, t dbSNP:111385796
1345 1345 a, g dbSNP:138263904
1346 1346 c, t dbSNP:192090590
1381 1381 a, c dbSNP:561929037
1382 1382 c, t dbSNP:11549319
1398 1398 c, g dbSNP:550296309
1400 1400 a, c dbSNP:141846451
1405 1405 g, t dbSNP:1803622
1417 1417 c, t dbSNP:759172931
1424 1424 a, g dbSNP:3211598
1448 1448 a, c dbSNP:3211599
1455 1455 c, t dbSNP:760001399
1468 1468 g, t dbSNP:552128740
1469 1469 a, g dbSNP:565707202
1476 1476 c, g dbSNP:771754711
1489 1489 a, g, t dbSNP:112433978

Target ORF information:

RefSeq Version NM_001289745
Organism Homo sapiens (human)
Definition Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001289745

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG 
GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
ATCTCTGCCC CCTCTGCTGA TGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTTGACGC TGGGGCTGGC
ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.