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CHMP2B charged multivesicular body protein 2B [Homo sapiens (human)]

Gene Symbol CHMP2B
Entrez Gene ID 25978
Full Name charged multivesicular body protein 2B
Synonyms ALS17, CHMP2.5, DMT1, VPS2-2, VPS2B
General protein information
Preferred Names
charged multivesicular body protein 2b
Names
charged multivesicular body protein 2b
VPS2 homolog B
chromatin modifying protein 2B
vacuolar protein-sorting-associated protein 2-2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p11.2

Summary This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609512

Disorder Html: Dementia, familial, nonspecific, 600795 (3); Amyotrophic lateral





The following CHMP2B gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHMP2B gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu66679 XM_011533576 PREDICTED: Homo sapiens charged multivesicular body protein 2B (CHMP2B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu18244 NM_014043 Homo sapiens charged multivesicular body protein 2B (CHMP2B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OHu18651 NM_001244644 Homo sapiens charged multivesicular body protein 2B (CHMP2B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu66679
Accession Version XM_011533576.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product charged multivesicular body protein 2b isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGATCTCGG CTCAACGCAA CCATCACCTC CCAGATTCAA GCGATTCTTC TACCTCAGCC 
TACCGAGTAG CTCGGATTAC AGATGTAATA AAGGAACAGA ATCGAGAGTT ACGAGGTACA
CAGAGGGCTA TAATCAGAGA TCGAGCAGCT TTAGAGAAAC AAGAAAAACA GCTGGAATTA
GAAATTAAGA AAATGGCCAA GATTGGTAAT AAGGAAGCTT GCAAAGTTTT AGCCAAACAA
CTTGTGCATC TACGGAAACA GAAGACGAGA ACTTTTGCTG TAAGTTCAAA AGTTACTTCT
ATGTCTACAC AAACAAAAGT GATGAATTCC CAAATGAAGA TGGCTGGAGC AATGTCTACT
ACAGCAAAAA CAATGCAGGC AGTTAACAAG AAGATGGATC CACAAAAGAC ATTACAAACA
ATGCAGAATT TCCAGAAGGA AAACATGAAA ATGGAAATGA CTGAAGAAAT GATCAATGAT
ACACTTGATG ACATCTTTGA CGGTTCTGAT GACGAAGAAG AAAGCCAGGA TATTGTGAAT
CAAGTTCTTG ATGAAATTGG AATTGAAATT TCTGGAAAGA TGGCCAAAGC TCCATCAGCT
GCTCGAAGCT TACCATCTGC CTCTACTTCA AAGGCTACAA TCTCAGATGA AGAGATTGAA
CGGCAACTCA AGGCTTTAGG AGTAGATTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011531878.1
CDS40..729
Misc Feature(1)133..630(+)
Translation
Position Chain Variation Link
14 14 a, g dbSNP:143175764
82 82 c, g dbSNP:560652090
97 97 c, g dbSNP:570267159
103 103 c, t dbSNP:113876409
105 105 a, g dbSNP:537021057
113 113 a, g dbSNP:180834245
122 122 a, t dbSNP:757730142
123 123 c, t dbSNP:141196445
124 124 -, g dbSNP:781126895
125 125 c, t dbSNP:781703226
126 126 a, g dbSNP:746197863
129 129 -, aaag dbSNP:747894141
133 133 a, g dbSNP:557194644
142 142 c, g, t dbSNP:780237778
143 143 a, g, t dbSNP:200322526
151 151 c, t dbSNP:138886714
152 152 a, g dbSNP:770968398
158 158 c, t dbSNP:776778264
172 172 a, g dbSNP:63750818
175 175 a, g dbSNP:139894940
177 177 a, c dbSNP:775142652
179 179 a, c dbSNP:762547677
181 181 c, t dbSNP:763615051
182 182 a, g dbSNP:374428152
195 195 c, g dbSNP:756803337
198 198 a, g dbSNP:376846232
199 199 a, c dbSNP:142645831
200 200 -, aaga dbSNP:774383512
200 200 -, aag dbSNP:769631869
201 201 a, g dbSNP:750983989
202 202 a, g dbSNP:151007324
203 203 a, g dbSNP:756409160
205 205 -, aaac dbSNP:746162849
214 214 g, t dbSNP:11540915
220 220 a, g dbSNP:756644379
227 227 a, g dbSNP:538072644
231 231 a, g dbSNP:754127865
244 244 c, g dbSNP:755409238
245 245 g, t dbSNP:370382342
248 248 a, g dbSNP:778990578
250 250 a, c dbSNP:188471297
262 262 a, g dbSNP:758486323
263 263 a, g dbSNP:143800051
274 274 a, c, g dbSNP:745754634
276 276 a, g dbSNP:575034954
279 279 a, g dbSNP:148750997
291 291 a, g dbSNP:768550735
292 292 c, t dbSNP:553846327
293 293 a, g dbSNP:200792883
300 300 a, c, g dbSNP:139126268
301 301 a, c dbSNP:772729869
302 302 a, g dbSNP:760226693
305 305 c, t dbSNP:192188850
306 306 a, g dbSNP:754433917
312 312 -, t dbSNP:762018031
314 314 g, t dbSNP:759763492
321 321 a, c, g dbSNP:765641845
335 335 c, t dbSNP:374277596
339 339 c, t dbSNP:144624883
344 344 c, g dbSNP:778194183
348 348 -, acaa dbSNP:765690978
354 354 a, g dbSNP:751663543
361 361 a, g dbSNP:757427735
379 379 a, g dbSNP:756918791
380 380 c, t dbSNP:779839985
386 386 c, g dbSNP:749259755
398 398 a, c dbSNP:281864934
399 399 c, t dbSNP:11540913
402 402 a, g dbSNP:778796624
404 404 c, g dbSNP:368704252
410 410 c, t dbSNP:753676349
420 420 a, g dbSNP:550496419
421 421 a, g dbSNP:778869364
426 426 c, g dbSNP:747904374
447 447 c, g dbSNP:758256432
448 448 a, g dbSNP:777390752
450 450 a, t dbSNP:746679698
451 451 c, t dbSNP:189313287
454 454 c, t dbSNP:776228632
459 459 a, c dbSNP:1044499
461 461 c, t dbSNP:770307338
482 482 a, g dbSNP:372014089
484 484 a, g dbSNP:763401641
495 495 a, g dbSNP:760063883
498 498 a, g dbSNP:764570832
499 499 a, c, t dbSNP:376941490
510 510 a, g dbSNP:202143621
513 513 c, g dbSNP:774798983
515 515 a, g dbSNP:63750944
529 529 g, t dbSNP:63750653
540 540 a, c, t dbSNP:767746876
541 541 c, g, t dbSNP:760794461
551 551 a, g dbSNP:375575660
552 552 c, t dbSNP:758354665
553 553 a, g dbSNP:764069353
565 565 a, c dbSNP:751339317
573 573 g, t dbSNP:757070412
580 580 c, t dbSNP:63750355
593 593 a, c dbSNP:780887155
609 609 c, t dbSNP:35414442
624 624 c, t dbSNP:753365110
626 626 a, c dbSNP:454175
630 630 c, t dbSNP:754600564
632 632 c, t dbSNP:369257752
635 635 c, t dbSNP:748616447
636 636 a, g dbSNP:143178463
643 643 c, t dbSNP:63751048
644 644 a, g dbSNP:747423794
646 646 a, c dbSNP:771113721
647 647 a, g dbSNP:78268395
648 648 c, t dbSNP:759602855
654 654 a, g dbSNP:187158507
668 668 c, t dbSNP:149380040
676 676 a, g dbSNP:761691146
679 679 a, c dbSNP:452942
700 700 c, t dbSNP:373536428
701 701 a, g dbSNP:750217435
705 705 a, c, t dbSNP:63751126
730 730 a, t dbSNP:570991078
736 736 g, t dbSNP:753600455
742 742 a, g dbSNP:754479715
747 747 a, g dbSNP:376791079
748 748 g, t dbSNP:764657705
755 755 c, t dbSNP:753199418
771 771 a, g dbSNP:758809463
779 779 a, c dbSNP:778385999
784 784 a, c dbSNP:407321
803 803 a, c dbSNP:202169483
807 807 a, g dbSNP:755253118
822 822 -, a dbSNP:368297778
881 881 a, g dbSNP:402375
922 922 c, t dbSNP:764379791
923 923 a, g dbSNP:541208359
940 940 a, g dbSNP:448316
960 960 c, t dbSNP:17189270
971 971 c, t dbSNP:550468260
995 995 a, g dbSNP:393439
997 997 a, g dbSNP:408793
1080 1080 a, c dbSNP:1044512
1109 1109 a, g dbSNP:371181321
1120 1120 a, g dbSNP:568774746
1146 1146 c, t dbSNP:535402661
1159 1159 a, g dbSNP:748575704
1172 1172 a, g dbSNP:114730892
1195 1195 a, t dbSNP:189471374
1218 1218 a, g dbSNP:390857
1272 1272 c, t dbSNP:1060230
1342 1342 a, c dbSNP:181525689
1343 1343 a, g dbSNP:384094
1386 1386 a, g dbSNP:139070624
1400 1400 a, c dbSNP:575796569
1413 1413 c, g dbSNP:762292080
1463 1463 g, t dbSNP:768051990
1473 1473 a, c dbSNP:185167991
1488 1488 c, t dbSNP:375678215
1500 1500 a, c dbSNP:199907485
1509 1509 a, g dbSNP:573607539
1523 1523 c, t dbSNP:373704153
1549 1549 a, g dbSNP:749981559
1552 1552 a, t dbSNP:541812197
1565 1565 c, t dbSNP:189927047
1567 1567 a, g dbSNP:527771370
1589 1589 c, t dbSNP:545943799
1590 1590 a, g dbSNP:755585268
1593 1593 a, g dbSNP:181777395
1597 1597 a, t dbSNP:10781
1604 1604 a, c dbSNP:11540916
1628 1628 a, t dbSNP:186472338
1633 1633 a, g dbSNP:550409585
1635 1635 c, t dbSNP:533971749
1647 1647 a, c dbSNP:533192743
1659 1659 a, g dbSNP:373565536
1679 1679 -, tg dbSNP:539957544
1761 1761 c, t dbSNP:753514950
1775 1775 c, t dbSNP:1062829
1786 1786 a, g dbSNP:550163111
1800 1800 a, g dbSNP:544589377
1825 1825 a, c dbSNP:529664158
1877 1877 a, g dbSNP:547345289
1910 1910 a, t dbSNP:565571322
1917 1917 c, t dbSNP:539432478
1919 1919 g, t dbSNP:115892684
1925 1925 c, g dbSNP:778579614
1946 1946 a, t dbSNP:569413438
1949 1949 a, g dbSNP:536809388
1968 1968 a, t dbSNP:773268462
1984 1984 g, t dbSNP:144167614
2096 2096 -, tg dbSNP:541295956
2097 2097 c, t dbSNP:747973849
2105 2105 a, c, g dbSNP:191203869
2120 2120 a, g dbSNP:758327175
2137 2137 a, g dbSNP:183429826
2138 2138 g, t dbSNP:188771358
2147 2147 g, t dbSNP:201231896
2151 2151 a, g dbSNP:572323799
2161 2161 g, t dbSNP:749581096
2194 2194 c, t dbSNP:745985475
2226 2226 a, g dbSNP:144158499
2230 2230 g, t dbSNP:191118931
2232 2232 c, g dbSNP:1060238
2282 2282 c, t dbSNP:544029932
2287 2287 c, t dbSNP:775653041
2290 2290 a, g dbSNP:562298465
2292 2292 a, g dbSNP:183335605
2302 2302 c, t dbSNP:774500018
2313 2313 c, t dbSNP:186567031
2317 2317 c, t dbSNP:11426
2318 2318 a, g dbSNP:1060241
2331 2331 c, t dbSNP:560015387
2339 2339 c, t dbSNP:191312397
2376 2376 a, g dbSNP:534772903
2382 2382 a, g dbSNP:183760081
2403 2403 a, c dbSNP:569401355

Target ORF information:

RefSeq Version XM_011533576
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens charged multivesicular body protein 2B (CHMP2B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533576

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGATCTCGG CTCAACGCAA CCATCACCTC CCAGATTCAA GCGATTCTTC TACCTCAGCC 
TACCGAGTAG CTCGGATTAC AGATGTAATA AAGGAACAGA ATCGAGAGTT ACGAGGTACA
CAGAGGGCTA TAATCAGAGA TCGAGCAGCT TTAGAGAAAC AAGAAAAACA GCTGGAATTA
GAAATTAAGA AAATGGCCAA GATTGGTAAT AAGGAAGCTT GCAAAGTTTT AGCCAAACAA
CTTGTGCATC TACGGAAACA GAAGACGAGA ACTTTTGCTG TAAGTTCAAA AGTTACTTCT
ATGTCTACAC AAACAAAAGT GATGAATTCC CAAATGAAGA TGGCTGGAGC AATGTCTACT
ACAGCAAAAA CAATGCAGGC AGTTAACAAG AAGATGGATC CACAAAAGAC ATTACAAACA
ATGCAGAATT TCCAGAAGGA AAACATGAAA ATGGAAATGA CTGAAGAAAT GATCAATGAT
ACACTTGATG ACATCTTTGA CGGTTCTGAT GACGAAGAAG AAAGCCAGGA TATTGTGAAT
CAAGTTCTTG ATGAAATTGG AATTGAAATT TCTGGAAAGA TGGCCAAAGC TCCATCAGCT
GCTCGAAGCT TACCATCTGC CTCTACTTCA AAGGCTACAA TCTCAGATGA AGAGATTGAA
CGGCAACTCA AGGCTTTAGG AGTAGATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18244
Accession Version NM_014043.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 642bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product charged multivesicular body protein 2b isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM828410.1, BC001553.1, DA426159.1 and BM982148.1. This sequence is a reference standard in the RefSeqGene project. On Mar 21, 2008 this sequence version replaced gi:40254865. Summary: This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002165.1, BC001553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGTCCC TCTTCAAGAA GAAAACCGTG GATGATGTAA TAAAGGAACA GAATCGAGAG 
TTACGAGGTA CACAGAGGGC TATAATCAGA GATCGAGCAG CTTTAGAGAA ACAAGAAAAA
CAGCTGGAAT TAGAAATTAA GAAAATGGCC AAGATTGGTA ATAAGGAAGC TTGCAAAGTT
TTAGCCAAAC AACTTGTGCA TCTACGGAAA CAGAAGACGA GAACTTTTGC TGTAAGTTCA
AAAGTTACTT CTATGTCTAC ACAAACAAAA GTGATGAATT CCCAAATGAA GATGGCTGGA
GCAATGTCTA CTACAGCAAA AACAATGCAG GCAGTTAACA AGAAGATGGA TCCACAAAAG
ACATTACAAA CAATGCAGAA TTTCCAGAAG GAAAACATGA AAATGGAAAT GACTGAAGAA
ATGATCAATG ATACACTTGA TGACATCTTT GACGGTTCTG ATGACGAAGA AGAAAGCCAG
GATATTGTGA ATCAAGTTCT TGATGAAATT GGAATTGAAA TTTCTGGAAA GATGGCCAAA
GCTCCATCAG CTGCTCGAAG CTTACCATCT GCCTCTACTT CAAAGGCTAC AATCTCAGAT
GAAGAGATTG AACGGCAACT CAAGGCTTTA GGAGTAGATT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_054762.2
CDS261..902
Misc Feature(1)75..77(+)
Misc Feature(2)264..266(+)
Misc Feature(3)306..803(+)
Misc Feature(4)855..857(+)
Misc Feature(5)855..857(+)
Misc Feature(6)855..857(+)
Misc Feature(7)861..893(+)
Exon (1)1..294
Gene:CHMP2B
Gene Synonym:
Exon (2)295..386
Gene:CHMP2B
Gene Synonym:
Exon (3)387..581
Gene:CHMP2B
Gene Synonym:
Exon (4)582..684
Gene:CHMP2B
Gene Synonym:
Exon (5)685..791
Gene:CHMP2B
Gene Synonym:
Exon (6)792..2628
Gene:CHMP2B
Gene Synonym:
Translation
Position Chain Variation Link
2 2 c, t dbSNP:538600777
56 56 c, t dbSNP:556556377
102 102 a, c dbSNP:574954693
110 110 a, c dbSNP:77328592
124 124 a, g dbSNP:766591028
135 135 c, t dbSNP:751723331
159 159 c, t dbSNP:36098294
166 166 a, c dbSNP:11540917
167 167 c, t dbSNP:750699594
175 175 c, g dbSNP:767269731
186 186 a, t dbSNP:756651997
207 207 a, c dbSNP:573036388
215 215 g, t dbSNP:778570281
217 217 a, g dbSNP:748625123
218 218 a, c dbSNP:772653746
224 224 a, c dbSNP:368463124
225 225 a, g dbSNP:773633818
225 225 -, g dbSNP:768627605
226 226 c, g dbSNP:11540914
227 227 a, g dbSNP:199854996
229 229 a, c, t dbSNP:776777126
230 230 a, g dbSNP:376821391
240 240 a, g dbSNP:775571812
243 243 c, t dbSNP:762989447
248 248 a, g dbSNP:200140890
249 249 c, t dbSNP:767454705
250 250 a, c dbSNP:750390377
251 251 c, g dbSNP:367697846
255 255 g, t dbSNP:766100499
259 259 c, t dbSNP:753473616
265 265 c, t dbSNP:754708487
276 276 a, t dbSNP:778515285
287 287 c, t dbSNP:2279720
295 295 a, t dbSNP:757730142
296 296 c, t dbSNP:141196445
297 297 -, g dbSNP:781126895
298 298 c, t dbSNP:781703226
299 299 a, g dbSNP:746197863
302 302 -, aaag dbSNP:747894141
306 306 a, g dbSNP:557194644
315 315 c, g, t dbSNP:780237778
316 316 a, g, t dbSNP:200322526
324 324 c, t dbSNP:138886714
325 325 a, g dbSNP:770968398
331 331 c, t dbSNP:776778264
345 345 a, g dbSNP:63750818
348 348 a, g dbSNP:139894940
350 350 a, c dbSNP:775142652
352 352 a, c dbSNP:762547677
354 354 c, t dbSNP:763615051
355 355 a, g dbSNP:374428152
368 368 c, g dbSNP:756803337
371 371 a, g dbSNP:376846232
372 372 a, c dbSNP:142645831
373 373 -, aaga dbSNP:774383512
373 373 -, aag dbSNP:769631869
374 374 a, g dbSNP:750983989
375 375 a, g dbSNP:151007324
376 376 a, g dbSNP:756409160
378 378 -, aaac dbSNP:746162849
387 387 g, t dbSNP:11540915
393 393 a, g dbSNP:756644379
400 400 a, g dbSNP:538072644
404 404 a, g dbSNP:754127865
417 417 c, g dbSNP:755409238
418 418 g, t dbSNP:370382342
421 421 a, g dbSNP:778990578
423 423 a, c dbSNP:188471297
435 435 a, g dbSNP:758486323
436 436 a, g dbSNP:143800051
447 447 a, c, g dbSNP:745754634
449 449 a, g dbSNP:575034954
452 452 a, g dbSNP:148750997
464 464 a, g dbSNP:768550735
465 465 c, t dbSNP:553846327
466 466 a, g dbSNP:200792883
473 473 a, c, g dbSNP:139126268
474 474 a, c dbSNP:772729869
475 475 a, g dbSNP:760226693
478 478 c, t dbSNP:192188850
479 479 a, g dbSNP:754433917
485 485 -, t dbSNP:762018031
487 487 g, t dbSNP:759763492
494 494 a, c, g dbSNP:765641845
508 508 c, t dbSNP:374277596
512 512 c, t dbSNP:144624883
517 517 c, g dbSNP:778194183
521 521 -, acaa dbSNP:765690978
527 527 a, g dbSNP:751663543
534 534 a, g dbSNP:757427735
552 552 a, g dbSNP:756918791
553 553 c, t dbSNP:779839985
559 559 c, g dbSNP:749259755
571 571 a, c dbSNP:281864934
572 572 c, t dbSNP:11540913
575 575 a, g dbSNP:778796624
577 577 c, g dbSNP:368704252
583 583 c, t dbSNP:753676349
593 593 a, g dbSNP:550496419
594 594 a, g dbSNP:778869364
599 599 c, g dbSNP:747904374
620 620 c, g dbSNP:758256432
621 621 a, g dbSNP:777390752
623 623 a, t dbSNP:746679698
624 624 c, t dbSNP:189313287
627 627 c, t dbSNP:776228632
632 632 a, c dbSNP:1044499
634 634 c, t dbSNP:770307338
655 655 a, g dbSNP:372014089
657 657 a, g dbSNP:763401641
668 668 a, g dbSNP:760063883
671 671 a, g dbSNP:764570832
672 672 a, c, t dbSNP:376941490
683 683 a, g dbSNP:202143621
686 686 c, g dbSNP:774798983
688 688 a, g dbSNP:63750944
702 702 g, t dbSNP:63750653
713 713 a, c, t dbSNP:767746876
714 714 c, g, t dbSNP:760794461
724 724 a, g dbSNP:375575660
725 725 c, t dbSNP:758354665
726 726 a, g dbSNP:764069353
738 738 a, c dbSNP:751339317
746 746 g, t dbSNP:757070412
753 753 c, t dbSNP:63750355
766 766 a, c dbSNP:780887155
782 782 c, t dbSNP:35414442
797 797 c, t dbSNP:753365110
799 799 a, c dbSNP:454175
803 803 c, t dbSNP:754600564
805 805 c, t dbSNP:369257752
808 808 c, t dbSNP:748616447
809 809 a, g dbSNP:143178463
816 816 c, t dbSNP:63751048
817 817 a, g dbSNP:747423794
819 819 a, c dbSNP:771113721
820 820 a, g dbSNP:78268395
821 821 c, t dbSNP:759602855
827 827 a, g dbSNP:187158507
841 841 c, t dbSNP:149380040
849 849 a, g dbSNP:761691146
852 852 a, c dbSNP:452942
873 873 c, t dbSNP:373536428
874 874 a, g dbSNP:750217435
878 878 a, c, t dbSNP:63751126
903 903 a, t dbSNP:570991078
909 909 g, t dbSNP:753600455
915 915 a, g dbSNP:754479715
920 920 a, g dbSNP:376791079
921 921 g, t dbSNP:764657705
928 928 c, t dbSNP:753199418
944 944 a, g dbSNP:758809463
952 952 a, c dbSNP:778385999
957 957 a, c dbSNP:407321
976 976 a, c dbSNP:202169483
980 980 a, g dbSNP:755253118
995 995 -, a dbSNP:368297778
1054 1054 a, g dbSNP:402375
1095 1095 c, t dbSNP:764379791
1096 1096 a, g dbSNP:541208359
1113 1113 a, g dbSNP:448316
1133 1133 c, t dbSNP:17189270
1144 1144 c, t dbSNP:550468260
1168 1168 a, g dbSNP:393439
1170 1170 a, g dbSNP:408793
1253 1253 a, c dbSNP:1044512
1282 1282 a, g dbSNP:371181321
1293 1293 a, g dbSNP:568774746
1319 1319 c, t dbSNP:535402661
1332 1332 a, g dbSNP:748575704
1345 1345 a, g dbSNP:114730892
1368 1368 a, t dbSNP:189471374
1391 1391 a, g dbSNP:390857
1445 1445 c, t dbSNP:1060230
1515 1515 a, c dbSNP:181525689
1516 1516 a, g dbSNP:384094
1559 1559 a, g dbSNP:139070624
1573 1573 a, c dbSNP:575796569
1586 1586 c, g dbSNP:762292080
1636 1636 g, t dbSNP:768051990
1646 1646 a, c dbSNP:185167991
1661 1661 c, t dbSNP:375678215
1673 1673 a, c dbSNP:199907485
1682 1682 a, g dbSNP:573607539
1696 1696 c, t dbSNP:373704153
1722 1722 a, g dbSNP:749981559
1725 1725 a, t dbSNP:541812197
1738 1738 c, t dbSNP:189927047
1740 1740 a, g dbSNP:527771370
1762 1762 c, t dbSNP:545943799
1763 1763 a, g dbSNP:755585268
1766 1766 a, g dbSNP:181777395
1770 1770 a, t dbSNP:10781
1777 1777 a, c dbSNP:11540916
1801 1801 a, t dbSNP:186472338
1806 1806 a, g dbSNP:550409585
1808 1808 c, t dbSNP:533971749
1820 1820 a, c dbSNP:533192743
1832 1832 a, g dbSNP:373565536
1852 1852 -, tg dbSNP:539957544
1934 1934 c, t dbSNP:753514950
1948 1948 c, t dbSNP:1062829
1959 1959 a, g dbSNP:550163111
1973 1973 a, g dbSNP:544589377
1998 1998 a, c dbSNP:529664158
2050 2050 a, g dbSNP:547345289
2083 2083 a, t dbSNP:565571322
2090 2090 c, t dbSNP:539432478
2092 2092 g, t dbSNP:115892684
2098 2098 c, g dbSNP:778579614
2119 2119 a, t dbSNP:569413438
2122 2122 a, g dbSNP:536809388
2141 2141 a, t dbSNP:773268462
2157 2157 g, t dbSNP:144167614
2269 2269 -, tg dbSNP:541295956
2270 2270 c, t dbSNP:747973849
2278 2278 a, c, g dbSNP:191203869
2293 2293 a, g dbSNP:758327175
2310 2310 a, g dbSNP:183429826
2311 2311 g, t dbSNP:188771358
2320 2320 g, t dbSNP:201231896
2324 2324 a, g dbSNP:572323799
2334 2334 g, t dbSNP:749581096
2367 2367 c, t dbSNP:745985475
2399 2399 a, g dbSNP:144158499
2403 2403 g, t dbSNP:191118931
2405 2405 c, g dbSNP:1060238
2455 2455 c, t dbSNP:544029932
2460 2460 c, t dbSNP:775653041
2463 2463 a, g dbSNP:562298465
2465 2465 a, g dbSNP:183335605
2475 2475 c, t dbSNP:774500018
2486 2486 c, t dbSNP:186567031
2490 2490 c, t dbSNP:11426
2491 2491 a, g dbSNP:1060241
2504 2504 c, t dbSNP:560015387
2512 2512 c, t dbSNP:191312397
2549 2549 a, g dbSNP:534772903
2555 2555 a, g dbSNP:183760081
2576 2576 a, c dbSNP:569401355

Target ORF information:

RefSeq Version NM_014043
Organism Homo sapiens (human)
Definition Homo sapiens charged multivesicular body protein 2B (CHMP2B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014043

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGTCCC TCTTCAAGAA GAAAACCGTG GATGATGTAA TAAAGGAACA GAATCGAGAG 
TTACGAGGTA CACAGAGGGC TATAATCAGA GATCGAGCAG CTTTAGAGAA ACAAGAAAAA
CAGCTGGAAT TAGAAATTAA GAAAATGGCC AAGATTGGTA ATAAGGAAGC TTGCAAAGTT
TTAGCCAAAC AACTTGTGCA TCTACGGAAA CAGAAGACGA GAACTTTTGC TGTAAGTTCA
AAAGTTACTT CTATGTCTAC ACAAACAAAA GTGATGAATT CCCAAATGAA GATGGCTGGA
GCAATGTCTA CTACAGCAAA AACAATGCAG GCAGTTAACA AGAAGATGGA TCCACAAAAG
ACATTACAAA CAATGCAGAA TTTCCAGAAG GAAAACATGA AAATGGAAAT GACTGAAGAA
ATGATCAATG ATACACTTGA TGACATCTTT GACGGTTCTG ATGACGAAGA AGAAAGCCAG
GATATTGTGA ATCAAGTTCT TGATGAAATT GGAATTGAAA TTTCTGGAAA GATGGCCAAA
GCTCCATCAG CTGCTCGAAG CTTACCATCT GCCTCTACTT CAAAGGCTAC AATCTCAGAT
GAAGAGATTG AACGGCAACT CAAGGCTTTA GGAGTAGATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18651
Accession Version NM_001244644.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product charged multivesicular body protein 2b isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM828410.1, AK296072.1, BI821848.1, AK002165.1 and BM982148.1. Summary: This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. The resulting predicted protein (isoform 2) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296072.1, BU857794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
ATGGAATTAG AAATTAAGAA AATGGCCAAG ATTGGTAATA AGGAAGCTTG CAAAGTTTTA 
GCCAAACAAC TTGTGCATCT ACGGAAACAG AAGACGAGAA CTTTTGCTGT AAGTTCAAAA
GTTACTTCTA TGTCTACACA AACAAAAGTG ATGAATTCCC AAATGAAGAT GGCTGGAGCA
ATGTCTACTA CAGCAAAAAC AATGCAGGCA GTTAACAAGA AGATGGATCC ACAAAAGACA
TTACAAACAA TGCAGAATTT CCAGAAGGAA AACATGAAAA TGGAAATGAC TGAAGAAATG
ATCAATGATA CACTTGATGA CATCTTTGAC GGTTCTGATG ACGAAGAAGA AAGCCAGGAT
ATTGTGAATC AAGTTCTTGA TGAAATTGGA ATTGAAATTT CTGGAAAGAT GGCCAAAGCT
CCATCAGCTG CTCGAAGCTT ACCATCTGCC TCTACTTCAA AGGCTACAAT CTCAGATGAA
GAGATTGAAC GGCAACTCAA GGCTTTAGGA GTAGATTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001231573.1
CDS292..810
Misc Feature(1)256..258(+)
Misc Feature(2)295..711(+)
Exon (1)1..294
Gene:CHMP2B
Gene Synonym:
Exon (2)295..489
Gene:CHMP2B
Gene Synonym:
Exon (3)490..592
Gene:CHMP2B
Gene Synonym:
Exon (4)593..699
Gene:CHMP2B
Gene Synonym:
Exon (5)700..2536
Gene:CHMP2B
Gene Synonym:
Translation
Position Chain Variation Link
2 2 c, t dbSNP:538600777
56 56 c, t dbSNP:556556377
102 102 a, c dbSNP:574954693
110 110 a, c dbSNP:77328592
124 124 a, g dbSNP:766591028
135 135 c, t dbSNP:751723331
159 159 c, t dbSNP:36098294
166 166 a, c dbSNP:11540917
167 167 c, t dbSNP:750699594
175 175 c, g dbSNP:767269731
186 186 a, t dbSNP:756651997
207 207 a, c dbSNP:573036388
215 215 g, t dbSNP:778570281
217 217 a, g dbSNP:748625123
218 218 a, c dbSNP:772653746
224 224 a, c dbSNP:368463124
225 225 a, g dbSNP:773633818
225 225 -, g dbSNP:768627605
226 226 c, g dbSNP:11540914
227 227 a, g dbSNP:199854996
229 229 a, c, t dbSNP:776777126
230 230 a, g dbSNP:376821391
240 240 a, g dbSNP:775571812
243 243 c, t dbSNP:762989447
248 248 a, g dbSNP:200140890
249 249 c, t dbSNP:767454705
250 250 a, c dbSNP:750390377
251 251 c, g dbSNP:367697846
255 255 g, t dbSNP:766100499
259 259 c, t dbSNP:753473616
265 265 c, t dbSNP:754708487
276 276 a, t dbSNP:778515285
287 287 c, t dbSNP:2279720
295 295 g, t dbSNP:11540915
301 301 a, g dbSNP:756644379
308 308 a, g dbSNP:538072644
312 312 a, g dbSNP:754127865
325 325 c, g dbSNP:755409238
326 326 g, t dbSNP:370382342
329 329 a, g dbSNP:778990578
331 331 a, c dbSNP:188471297
343 343 a, g dbSNP:758486323
344 344 a, g dbSNP:143800051
355 355 a, c, g dbSNP:745754634
357 357 a, g dbSNP:575034954
360 360 a, g dbSNP:148750997
372 372 a, g dbSNP:768550735
373 373 c, t dbSNP:553846327
374 374 a, g dbSNP:200792883
381 381 a, c, g dbSNP:139126268
382 382 a, c dbSNP:772729869
383 383 a, g dbSNP:760226693
386 386 c, t dbSNP:192188850
387 387 a, g dbSNP:754433917
393 393 -, t dbSNP:762018031
395 395 g, t dbSNP:759763492
402 402 a, c, g dbSNP:765641845
416 416 c, t dbSNP:374277596
420 420 c, t dbSNP:144624883
425 425 c, g dbSNP:778194183
429 429 -, acaa dbSNP:765690978
435 435 a, g dbSNP:751663543
442 442 a, g dbSNP:757427735
460 460 a, g dbSNP:756918791
461 461 c, t dbSNP:779839985
467 467 c, g dbSNP:749259755
479 479 a, c dbSNP:281864934
480 480 c, t dbSNP:11540913
483 483 a, g dbSNP:778796624
485 485 c, g dbSNP:368704252
491 491 c, t dbSNP:753676349
501 501 a, g dbSNP:550496419
502 502 a, g dbSNP:778869364
507 507 c, g dbSNP:747904374
528 528 c, g dbSNP:758256432
529 529 a, g dbSNP:777390752
531 531 a, t dbSNP:746679698
532 532 c, t dbSNP:189313287
535 535 c, t dbSNP:776228632
540 540 a, c dbSNP:1044499
542 542 c, t dbSNP:770307338
563 563 a, g dbSNP:372014089
565 565 a, g dbSNP:763401641
576 576 a, g dbSNP:760063883
579 579 a, g dbSNP:764570832
580 580 a, c, t dbSNP:376941490
591 591 a, g dbSNP:202143621
594 594 c, g dbSNP:774798983
596 596 a, g dbSNP:63750944
610 610 g, t dbSNP:63750653
621 621 a, c, t dbSNP:767746876
622 622 c, g, t dbSNP:760794461
632 632 a, g dbSNP:375575660
633 633 c, t dbSNP:758354665
634 634 a, g dbSNP:764069353
646 646 a, c dbSNP:751339317
654 654 g, t dbSNP:757070412
661 661 c, t dbSNP:63750355
674 674 a, c dbSNP:780887155
690 690 c, t dbSNP:35414442
705 705 c, t dbSNP:753365110
707 707 a, c dbSNP:454175
711 711 c, t dbSNP:754600564
713 713 c, t dbSNP:369257752
716 716 c, t dbSNP:748616447
717 717 a, g dbSNP:143178463
724 724 c, t dbSNP:63751048
725 725 a, g dbSNP:747423794
727 727 a, c dbSNP:771113721
728 728 a, g dbSNP:78268395
729 729 c, t dbSNP:759602855
735 735 a, g dbSNP:187158507
749 749 c, t dbSNP:149380040
757 757 a, g dbSNP:761691146
760 760 a, c dbSNP:452942
781 781 c, t dbSNP:373536428
782 782 a, g dbSNP:750217435
786 786 a, c, t dbSNP:63751126
811 811 a, t dbSNP:570991078
817 817 g, t dbSNP:753600455
823 823 a, g dbSNP:754479715
828 828 a, g dbSNP:376791079
829 829 g, t dbSNP:764657705
836 836 c, t dbSNP:753199418
852 852 a, g dbSNP:758809463
860 860 a, c dbSNP:778385999
865 865 a, c dbSNP:407321
884 884 a, c dbSNP:202169483
888 888 a, g dbSNP:755253118
903 903 -, a dbSNP:368297778
962 962 a, g dbSNP:402375
1003 1003 c, t dbSNP:764379791
1004 1004 a, g dbSNP:541208359
1021 1021 a, g dbSNP:448316
1041 1041 c, t dbSNP:17189270
1052 1052 c, t dbSNP:550468260
1076 1076 a, g dbSNP:393439
1078 1078 a, g dbSNP:408793
1161 1161 a, c dbSNP:1044512
1190 1190 a, g dbSNP:371181321
1201 1201 a, g dbSNP:568774746
1227 1227 c, t dbSNP:535402661
1240 1240 a, g dbSNP:748575704
1253 1253 a, g dbSNP:114730892
1276 1276 a, t dbSNP:189471374
1299 1299 a, g dbSNP:390857
1353 1353 c, t dbSNP:1060230
1423 1423 a, c dbSNP:181525689
1424 1424 a, g dbSNP:384094
1467 1467 a, g dbSNP:139070624
1481 1481 a, c dbSNP:575796569
1494 1494 c, g dbSNP:762292080
1544 1544 g, t dbSNP:768051990
1554 1554 a, c dbSNP:185167991
1569 1569 c, t dbSNP:375678215
1581 1581 a, c dbSNP:199907485
1590 1590 a, g dbSNP:573607539
1604 1604 c, t dbSNP:373704153
1630 1630 a, g dbSNP:749981559
1633 1633 a, t dbSNP:541812197
1646 1646 c, t dbSNP:189927047
1648 1648 a, g dbSNP:527771370
1670 1670 c, t dbSNP:545943799
1671 1671 a, g dbSNP:755585268
1674 1674 a, g dbSNP:181777395
1678 1678 a, t dbSNP:10781
1685 1685 a, c dbSNP:11540916
1709 1709 a, t dbSNP:186472338
1714 1714 a, g dbSNP:550409585
1716 1716 c, t dbSNP:533971749
1728 1728 a, c dbSNP:533192743
1740 1740 a, g dbSNP:373565536
1760 1760 -, tg dbSNP:539957544
1842 1842 c, t dbSNP:753514950
1856 1856 c, t dbSNP:1062829
1867 1867 a, g dbSNP:550163111
1881 1881 a, g dbSNP:544589377
1906 1906 a, c dbSNP:529664158
1958 1958 a, g dbSNP:547345289
1991 1991 a, t dbSNP:565571322
1998 1998 c, t dbSNP:539432478
2000 2000 g, t dbSNP:115892684
2006 2006 c, g dbSNP:778579614
2027 2027 a, t dbSNP:569413438
2030 2030 a, g dbSNP:536809388
2049 2049 a, t dbSNP:773268462
2065 2065 g, t dbSNP:144167614
2177 2177 -, tg dbSNP:541295956
2178 2178 c, t dbSNP:747973849
2186 2186 a, c, g dbSNP:191203869
2201 2201 a, g dbSNP:758327175
2218 2218 a, g dbSNP:183429826
2219 2219 g, t dbSNP:188771358
2228 2228 g, t dbSNP:201231896
2232 2232 a, g dbSNP:572323799
2242 2242 g, t dbSNP:749581096
2275 2275 c, t dbSNP:745985475
2307 2307 a, g dbSNP:144158499
2311 2311 g, t dbSNP:191118931
2313 2313 c, g dbSNP:1060238
2363 2363 c, t dbSNP:544029932
2368 2368 c, t dbSNP:775653041
2371 2371 a, g dbSNP:562298465
2373 2373 a, g dbSNP:183335605
2383 2383 c, t dbSNP:774500018
2394 2394 c, t dbSNP:186567031
2398 2398 c, t dbSNP:11426
2399 2399 a, g dbSNP:1060241
2412 2412 c, t dbSNP:560015387
2420 2420 c, t dbSNP:191312397
2457 2457 a, g dbSNP:534772903
2463 2463 a, g dbSNP:183760081
2484 2484 a, c dbSNP:569401355

Target ORF information:

RefSeq Version NM_001244644
Organism Homo sapiens (human)
Definition Homo sapiens charged multivesicular body protein 2B (CHMP2B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001244644

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGAATTAG AAATTAAGAA AATGGCCAAG ATTGGTAATA AGGAAGCTTG CAAAGTTTTA 
GCCAAACAAC TTGTGCATCT ACGGAAACAG AAGACGAGAA CTTTTGCTGT AAGTTCAAAA
GTTACTTCTA TGTCTACACA AACAAAAGTG ATGAATTCCC AAATGAAGAT GGCTGGAGCA
ATGTCTACTA CAGCAAAAAC AATGCAGGCA GTTAACAAGA AGATGGATCC ACAAAAGACA
TTACAAACAA TGCAGAATTT CCAGAAGGAA AACATGAAAA TGGAAATGAC TGAAGAAATG
ATCAATGATA CACTTGATGA CATCTTTGAC GGTTCTGATG ACGAAGAAGA AAGCCAGGAT
ATTGTGAATC AAGTTCTTGA TGAAATTGGA ATTGAAATTT CTGGAAAGAT GGCCAAAGCT
CCATCAGCTG CTCGAAGCTT ACCATCTGCC TCTACTTCAA AGGCTACAAT CTCAGATGAA
GAGATTGAAC GGCAACTCAA GGCTTTAGGA GTAGATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.