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PLA2G2D cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLA2G2D
Entrez Gene ID 26279
Full Name phospholipase A2, group IID
Synonyms PLA2IID, SPLASH, sPLA2-IID, sPLA2S
General protein information
Preferred Names
group IID secretory phospholipase A2
Names
group IID secretory phospholipase A2
GIID sPLA2
secretory phospholipase A2s
phosphatidylcholine 2-acylhydrolase 2D
secretory-type PLA, stroma-associated homolog
NP_036532.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.12

Summary This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]. lac of sum
Disorder MIM:

605630


mRNA and Protein(s)

mRNA Protein Name
NM_012400 NP_036532 group IID secretory phospholipase A2 isoform 1 precursor
NM_001271814 NP_001258743 group IID secretory phospholipase A2 isoform 2 precursor



Homo sapiens (human) PLA2G2D NP_036532.1
Pan troglodytes (chimpanzee) PLA2G2D XP_524585.3
Macaca mulatta (Rhesus monkey) PLA2G2D XP_001095528.1
Canis lupus familiaris (dog) PLA2G2D XP_544524.3
Mus musculus (house mouse) Pla2g2d NP_035239.1
Rattus norvegicus (Norway rat) Pla2g2d NP_001013446.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLA2G2D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G2D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu10091
NM_012400 Homo sapiens phospholipase A2, group IID (PLA2G2D), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu09864
NM_001271814 Homo sapiens phospholipase A2, group IID (PLA2G2D), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu10091
Clone ID Related Accession (Same CDS sequence) NM_012400
Accession Version NM_012400.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product group IID secretory phospholipase A2 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, BC025706.1 and AK026347.1. On Nov 28, 2012 this sequence version replaced gi:21314652. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:10455175) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AF188625.1, AK290406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2155628 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036532.1
CDS69..506
Misc Feature(1)129..482(+)
Misc Feature(2)132..314(+)
Misc Feature(3)207..272(+)
Misc Feature(4)213..404(+)
Exon (1)1..108
Gene:PLA2G2D
Gene Synonym:
Exon (2)109..253
Gene:PLA2G2D
Gene Synonym:
Exon (3)254..360
Gene:PLA2G2D
Gene Synonym:
Exon (4)361..1970
Gene:PLA2G2D
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_012400
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase A2, group IID (PLA2G2D), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_012400

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09864
Clone ID Related Accession (Same CDS sequence) NM_001271814
Accession Version NM_001271814.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 189bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product group IID secretory phospholipase A2 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, AK310156.1, BC025706.1 and AK026347.1. Transcript Variant: This variant (2) lacks an internal exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:10455175) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK310156.1, DA982802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258743.1
CDS69..257
Misc Feature(1)129..>254(+)
Misc Feature(2)207..221(+)
Exon (1)1..108
Gene:PLA2G2D
Gene Synonym:
Exon (2)109..253
Gene:PLA2G2D
Gene Synonym:
Exon (3)254..1863
Gene:PLA2G2D
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001271814
Organism Homo sapiens (human)
Definition Homo sapiens phospholipase A2, group IID (PLA2G2D), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271814

ORF Insert Sequence:

1
61
121
181
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry
PLoS ONE 8 (3), E59267 (2013)
Hsu YH, Dumlao DS, Cao J and Dennis EA.


book

Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa
J. Androl. 33 (5), 975-983 (2012)
Li K, Jin JY, Chen WY, Shi QX, Ni Y and Roldan ER.


book

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities
Biochimie 94 (6), 1437-1440 (2012)
Fonseca-Maldonado R, Ferreira TL and Ward RJ.


book

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population
PLoS ONE 7 (12), E51954 (2012)
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA and Butte NF.


book

Gly80Ser polymorphism of phospholipase A2-IID is associated with cytokine inducibility in A549 cells
Respiration 78 (3), 312-321 (2009)
Igarashi A, Shibata Y, Yamauchi K, Osaka D, Takabatake N, Abe S, Inoue S, Kimura T, Yamaguchi Y, Ishizaki J, Hanasaki K and Kubota I.


book

Increased hepatic cholesterol accumulation in transgenic mice overexpressing human secretory phospholipase A2 group IIA
Inflammation 28 (2), 59-65 (2004)
Eckey R, Menschikowski M, Lattke P and Jaross W.


book

Regulation of arachidonic acid availability for eicosanoid production
Biochem. Cell Biol. 82 (1), 1-17 (2004)
Leslie CC.


book

Allelic association study between phospholipase A2 genes and bipolar affective disorder
Bipolar Disord 5 (4), 295-299 (2003)
Meira-Lima I, Jardim D, Junqueira R, Ikenaga E and Vallada H.


book

Increased gene expression of novel cytosolic and secretory phospholipase A(2) types in human airway epithelial cells induced by tumor necrosis factor-alpha and IFN-gamma
J. Interferon Cytokine Res. 22 (9), 947-955 (2002)
Lindbom J, Ljungman AG, Lindahl M and Tagesson C.


book

Cloning and characterization of novel mouse and human secretory phospholipase A(2)s
J. Biol. Chem. 274 (35), 24973-24979 (1999)
Ishizaki J, Suzuki N, Higashino K, Yokota Y, Ono T, Kawamoto K, Fujii N, Arita H and Hanasaki K.


 
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