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GCK cDNA ORF clone, Homo sapiens (human)

Gene Symbol GCK
Entrez Gene ID 2645
Full Name glucokinase (hexokinase 4)
Synonyms FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2
General protein information
Preferred Names
glucokinase
Names
glucokinase
HK IV
hexokinase-4
hexokinase-D
hexokinase type IV
hexokinase D, pancreatic isozyme
ATP:D-hexose 6-phosphotransferase
NP_000153.1
NP_277042.1
NP_277043.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7p15.3-p15.1

Summary Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]. lac of sum
Disorder MIM:

138079

Disorder Html: MODY, type II, 125851 (3); Diabetes mellitus, noninsulin-dependent,

mRNA and Protein(s)

mRNA Protein Name
NM_000162 NP_000153 glucokinase isoform 1
NM_033507 NP_277042 glucokinase isoform 2
NM_033508 NP_277043 glucokinase isoform 3



Homo sapiens (human) GCK NP_000153.1
Pan troglodytes (chimpanzee) GCK XP_001143302.1
Macaca mulatta (Rhesus monkey) GCK XP_001093035.2
Bos taurus (cattle) GCK NP_001095772.1
Mus musculus (house mouse) Gck NP_034422.2
Rattus norvegicus (Norway rat) Gck NP_036697.1
Gallus gallus (chicken) GCK XP_427930.4
Danio rerio (zebrafish) gck NP_001038850.2
Xenopus (Silurana) tropicalis (western clawed frog) gck NP_001096321.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GCK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18369
NM_000162 Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu17640 NM_033507 Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $272.30
$389.00
OHu17606 NM_033508 Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $272.30
$389.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18369
Clone ID Related Accession (Same CDS sequence) NM_000162
Accession Version NM_000162.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucokinase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001890.1 and M90299.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2008 this sequence version replaced gi:15967157. Transcript Variant: This variant (1) encodes the isoform expressed specifically in pancreatic islet beta cells. Its first exon is specific to this variant, which has a unique 5' UTR. Isoform 1 has a distinct N-terminus; the remainder of the protein is identical to isoforms 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001890.1, M88011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000153.1
CDS471..1868
Misc Feature(1)375..377(+)
Misc Feature(2)492..1844(+)
Misc Feature(3)693..1304(+)
Misc Feature(4)702..1154(+)
Misc Feature(5)921..926(+)
Misc Feature(6)972..977(+)
Misc Feature(7)1080..1085(+)
Misc Feature(8)1125..1838(+)
Exon (1)1..515
Gene:GCK
Gene Synonym:
Exon (2)516..678
Gene:GCK
Gene Synonym:
Exon (3)679..833
Gene:GCK
Gene Synonym:
Exon (4)834..953
Gene:GCK
Gene Synonym:
Exon (5)954..1049
Gene:GCK
Gene Synonym:
Exon (6)1050..1149
Gene:GCK
Gene Synonym:
Exon (7)1150..1333
Gene:GCK
Gene Synonym:
Exon (8)1334..1489
Gene:GCK
Gene Synonym:
Exon (9)1490..1723
Gene:GCK
Gene Synonym:
Exon (10)1724..2733
Gene:GCK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000162
Organism Homo sapiens (human)
Definition Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000162

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCTGGACG ACAGAGCCAG GATGGAGGCC GCCAAGAAGG AGAAGGTAGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAGGACCTG AAGAAGGTGA TGAGACGGAT GCAGAAGGAG
ATGGACCGCG GCCTGAGGCT GGAGACCCAT GAAGAGGCCA GTGTGAAGAT GCTGCCCACC
TACGTGCGCT CCACCCCAGA AGGCTCAGAA GTCGGGGACT TCCTCTCCCT GGACCTGGGT
GGCACTAACT TCAGGGTGAT GCTGGTGAAG GTGGGAGAAG GTGAGGAGGG GCAGTGGAGC
GTGAAGACCA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACCGG CACTGCTGAG
ATGCTCTTCG ACTACATCTC TGAGTGCATC TCCGACTTCC TGGACAAGCA TCAGATGAAA
CACAAGAAGC TGCCCCTGGG CTTCACCTTC TCCTTTCCTG TGAGGCACGA AGACATCGAT
AAGGGCATCC TTCTCAACTG GACCAAGGGC TTCAAGGCCT CAGGAGCAGA AGGGAACAAT
GTCGTGGGGC TTCTGCGAGA CGCTATCAAA CGGAGAGGGG ACTTTGAAAT GGATGTGGTG
GCAATGGTGA ATGACACGGT GGCCACGATG ATCTCCTGCT ACTACGAAGA CCATCAGTGC
GAGGTCGGCA TGATCGTGGG CACGGGCTGC AATGCCTGCT ACATGGAGGA GATGCAGAAT
GTGGAGCTGG TGGAGGGGGA CGAGGGCCGC ATGTGCGTCA ATACCGAGTG GGGCGCCTTC
GGGGACTCCG GCGAGCTGGA CGAGTTCCTG CTGGAGTATG ACCGCCTGGT GGACGAGAGC
TCTGCAAACC CCGGTCAGCA GCTGTATGAG AAGCTCATAG GTGGCAAGTA CATGGGCGAG
CTGGTGCGGC TTGTGCTGCT CAGGCTCGTG GACGAAAACC TGCTCTTCCA CGGGGAGGCC
TCCGAGCAGC TGCGCACACG CGGAGCCTTC GAGACGCGCT TCGTGTCGCA GGTGGAGAGC
GACACGGGCG ACCGCAAGCA GATCTACAAC ATCCTGAGCA CGCTGGGGCT GCGACCCTCG
ACCACCGACT GCGACATCGT GCGCCGCGCC TGCGAGAGCG TGTCTACGCG CGCTGCGCAC
ATGTGCTCGG CGGGGCTGGC GGGCGTCATC AACCGCATGC GCGAGAGCCG CAGCGAGGAC
GTAATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC CAGCTTCAAG
GAGCGGTTCC ATGCCAGCGT GCGCAGGCTG ACGCCCAGCT GCGAGATCAC CTTCATCGAG
TCGGAGGAGG GCAGTGGCCG GGGCGCGGCC CTGGTCTCGG CGGTGGCCTG TAAGAAGGCC
TGTATGCTGG GCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17640
Clone ID Related Accession (Same CDS sequence) NM_033507
Accession Version NM_033507.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucokinase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4, AK122876.1, M69051.1, CD251038.1 and M90299.1. Transcript Variant: This variant (2) encodes the major isoform expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3, but it lacks a second liver-specific exon found in variant 3. Isoform 2 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_277042.1
CDS169..1569
Misc Feature(1)154..156(+)
Misc Feature(2)217..1545(+)
Misc Feature(3)394..1005(+)
Misc Feature(4)403..855(+)
Misc Feature(5)826..1539(+)
Exon (1)1..216
Gene:GCK
Gene Synonym:
Exon (2)217..379
Gene:GCK
Gene Synonym:
Exon (3)380..534
Gene:GCK
Gene Synonym:
Exon (4)535..654
Gene:GCK
Gene Synonym:
Exon (5)655..750
Gene:GCK
Gene Synonym:
Exon (6)751..850
Gene:GCK
Gene Synonym:
Exon (7)851..1034
Gene:GCK
Gene Synonym:
Exon (8)1035..1190
Gene:GCK
Gene Synonym:
Exon (9)1191..1424
Gene:GCK
Gene Synonym:
Exon (10)1425..2434
Gene:GCK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033507
Organism Homo sapiens (human)
Definition Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033507

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGATGG ATGTCACAAG GAGCCAGGCC CAGACAGCCT TGACTCTGGT AGAGCAGATC 
CTGGCAGAGT TCCAGCTGCA GGAGGAGGAC CTGAAGAAGG TGATGAGACG GATGCAGAAG
GAGATGGACC GCGGCCTGAG GCTGGAGACC CATGAAGAGG CCAGTGTGAA GATGCTGCCC
ACCTACGTGC GCTCCACCCC AGAAGGCTCA GAAGTCGGGG ACTTCCTCTC CCTGGACCTG
GGTGGCACTA ACTTCAGGGT GATGCTGGTG AAGGTGGGAG AAGGTGAGGA GGGGCAGTGG
AGCGTGAAGA CCAAACACCA GATGTACTCC ATCCCCGAGG ACGCCATGAC CGGCACTGCT
GAGATGCTCT TCGACTACAT CTCTGAGTGC ATCTCCGACT TCCTGGACAA GCATCAGATG
AAACACAAGA AGCTGCCCCT GGGCTTCACC TTCTCCTTTC CTGTGAGGCA CGAAGACATC
GATAAGGGCA TCCTTCTCAA CTGGACCAAG GGCTTCAAGG CCTCAGGAGC AGAAGGGAAC
AATGTCGTGG GGCTTCTGCG AGACGCTATC AAACGGAGAG GGGACTTTGA AATGGATGTG
GTGGCAATGG TGAATGACAC GGTGGCCACG ATGATCTCCT GCTACTACGA AGACCATCAG
TGCGAGGTCG GCATGATCGT GGGCACGGGC TGCAATGCCT GCTACATGGA GGAGATGCAG
AATGTGGAGC TGGTGGAGGG GGACGAGGGC CGCATGTGCG TCAATACCGA GTGGGGCGCC
TTCGGGGACT CCGGCGAGCT GGACGAGTTC CTGCTGGAGT ATGACCGCCT GGTGGACGAG
AGCTCTGCAA ACCCCGGTCA GCAGCTGTAT GAGAAGCTCA TAGGTGGCAA GTACATGGGC
GAGCTGGTGC GGCTTGTGCT GCTCAGGCTC GTGGACGAAA ACCTGCTCTT CCACGGGGAG
GCCTCCGAGC AGCTGCGCAC ACGCGGAGCC TTCGAGACGC GCTTCGTGTC GCAGGTGGAG
AGCGACACGG GCGACCGCAA GCAGATCTAC AACATCCTGA GCACGCTGGG GCTGCGACCC
TCGACCACCG ACTGCGACAT CGTGCGCCGC GCCTGCGAGA GCGTGTCTAC GCGCGCTGCG
CACATGTGCT CGGCGGGGCT GGCGGGCGTC ATCAACCGCA TGCGCGAGAG CCGCAGCGAG
GACGTAATGC GCATCACTGT GGGCGTGGAT GGCTCCGTGT ACAAGCTGCA CCCCAGCTTC
AAGGAGCGGT TCCATGCCAG CGTGCGCAGG CTGACGCCCA GCTGCGAGAT CACCTTCATC
GAGTCGGAGG AGGGCAGTGG CCGGGGCGCG GCCCTGGTCT CGGCGGTGGC CTGTAAGAAG
GCCTGTATGC TGGGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17606
Clone ID Related Accession (Same CDS sequence) NM_033508
Accession Version NM_033508.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucokinase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006454.4, M69051.1, DA640823.1, CD251038.1 and M90299.1. Transcript Variant: This variant (3) is the minor form expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3; its second exon is unique to this transcript. Isoform 3 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M69051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162895 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_277043.1
CDS299..1693
Misc Feature(1)230..232(+)
Misc Feature(2)335..1669(+)
Misc Feature(3)518..1129(+)
Misc Feature(4)527..979(+)
Misc Feature(5)950..1663(+)
Exon (1)1..216
Gene:GCK
Gene Synonym:
Exon (2)217..340
Gene:GCK
Gene Synonym:
Exon (3)341..503
Gene:GCK
Gene Synonym:
Exon (4)504..658
Gene:GCK
Gene Synonym:
Exon (5)659..778
Gene:GCK
Gene Synonym:
Exon (6)779..874
Gene:GCK
Gene Synonym:
Exon (7)875..974
Gene:GCK
Gene Synonym:
Exon (8)975..1158
Gene:GCK
Gene Synonym:
Exon (9)1159..1314
Gene:GCK
Gene Synonym:
Exon (10)1315..1548
Gene:GCK
Gene Synonym:
Exon (11)1549..2558
Gene:GCK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_033508
Organism Homo sapiens (human)
Definition Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033508

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCCAGAC CAAGATCCCA ACTCCCACAA CCCAACTCCC AGGTAGAGCA GATCCTGGCA 
GAGTTCCAGC TGCAGGAGGA GGACCTGAAG AAGGTGATGA GACGGATGCA GAAGGAGATG
GACCGCGGCC TGAGGCTGGA GACCCATGAA GAGGCCAGTG TGAAGATGCT GCCCACCTAC
GTGCGCTCCA CCCCAGAAGG CTCAGAAGTC GGGGACTTCC TCTCCCTGGA CCTGGGTGGC
ACTAACTTCA GGGTGATGCT GGTGAAGGTG GGAGAAGGTG AGGAGGGGCA GTGGAGCGTG
AAGACCAAAC ACCAGATGTA CTCCATCCCC GAGGACGCCA TGACCGGCAC TGCTGAGATG
CTCTTCGACT ACATCTCTGA GTGCATCTCC GACTTCCTGG ACAAGCATCA GATGAAACAC
AAGAAGCTGC CCCTGGGCTT CACCTTCTCC TTTCCTGTGA GGCACGAAGA CATCGATAAG
GGCATCCTTC TCAACTGGAC CAAGGGCTTC AAGGCCTCAG GAGCAGAAGG GAACAATGTC
GTGGGGCTTC TGCGAGACGC TATCAAACGG AGAGGGGACT TTGAAATGGA TGTGGTGGCA
ATGGTGAATG ACACGGTGGC CACGATGATC TCCTGCTACT ACGAAGACCA TCAGTGCGAG
GTCGGCATGA TCGTGGGCAC GGGCTGCAAT GCCTGCTACA TGGAGGAGAT GCAGAATGTG
GAGCTGGTGG AGGGGGACGA GGGCCGCATG TGCGTCAATA CCGAGTGGGG CGCCTTCGGG
GACTCCGGCG AGCTGGACGA GTTCCTGCTG GAGTATGACC GCCTGGTGGA CGAGAGCTCT
GCAAACCCCG GTCAGCAGCT GTATGAGAAG CTCATAGGTG GCAAGTACAT GGGCGAGCTG
GTGCGGCTTG TGCTGCTCAG GCTCGTGGAC GAAAACCTGC TCTTCCACGG GGAGGCCTCC
GAGCAGCTGC GCACACGCGG AGCCTTCGAG ACGCGCTTCG TGTCGCAGGT GGAGAGCGAC
ACGGGCGACC GCAAGCAGAT CTACAACATC CTGAGCACGC TGGGGCTGCG ACCCTCGACC
ACCGACTGCG ACATCGTGCG CCGCGCCTGC GAGAGCGTGT CTACGCGCGC TGCGCACATG
TGCTCGGCGG GGCTGGCGGG CGTCATCAAC CGCATGCGCG AGAGCCGCAG CGAGGACGTA
ATGCGCATCA CTGTGGGCGT GGATGGCTCC GTGTACAAGC TGCACCCCAG CTTCAAGGAG
CGGTTCCATG CCAGCGTGCG CAGGCTGACG CCCAGCTGCG AGATCACCTT CATCGAGTCG
GAGGAGGGCA GTGGCCGGGG CGCGGCCCTG GTCTCGGCGG TGGCCTGTAA GAAGGCCTGT
ATGCTGGGCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Prevalence of vascular complications among patients with glucokinase mutations and prolonged, mild hyperglycemia
JAMA 311 (3), 279-286 (2014)
Steele AM, Shields BM, Wensley KJ, Colclough K, Ellard S and Hattersley AT.


book

Structural basis for regulation of human glucokinase by glucokinase regulatory protein
Biochemistry 52 (36), 6232-6239 (2013)
Beck T and Miller BG.


book

Maturity onset diabetes of the young: identification and diagnosis
Ann. Clin. Biochem. 50 (PT 5), 403-415 (2013)
McDonald TJ and Ellard S.


book

Large scale meta-analyses of fasting plasma glucose raising variants in GCK, GCKR, MTNR1B and G6PC2 and their impacts on type 2 diabetes mellitus risk
PLoS ONE 8 (6), E67665 (2013)
Wang H, Liu L, Zhao J, Cui G, Chen C, Ding H and Wang DW.


book

Permanent Neonatal Diabetes Mellitus
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
De Leon,D.D. and Stanley,C.A.


book

Familial Hyperinsulinism
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Glaser,B.


book

Nonsense mutation of glucokinase gene in late-onset non-insulin-dependent diabetes mellitus
Lancet 340 (8831), 1316-1317 (1992)
Katagiri H, Asano T, Ishihara H, Inukai K, Anai M, Miyazaki J, Tsukuda K, Kikuchi M, Yazaki Y and Oka Y.


book

Missense glucokinase mutation in maturity-onset diabetes of the young and mutation screening in late-onset diabetes
Nat. Genet. 2 (2), 153-156 (1992)
Stoffel M, Patel P, Lo YM, Hattersley AT, Lucassen AM, Page R, Bell JI, Bell GI, Turner RC and Wainscoat JS.


book

Human glucokinase gene: isolation, structural characterization, and identification of a microsatellite repeat polymorphism
Mol. Endocrinol. 6 (7), 1070-1081 (1992)
Tanizawa Y, Matsutani A, Chiu KC and Permutt MA.


book

Linkage of type 2 diabetes to the glucokinase gene
Lancet 339 (8805), 1307-1310 (1992)
Hattersley AT, Turner RC, Permutt MA, Patel P, Tanizawa Y, Chiu KC, O'Rahilly S, Watkins PJ and Wainscoat JS.


 
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