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GEM cDNA ORF clone, Homo sapiens (human)

Gene Symbol GEM
Entrez Gene ID 2669
Full Name GTP binding protein overexpressed in skeletal muscle
Synonyms KIR
General protein information
Preferred Names
GTP-binding protein GEM
Names
GTP-binding protein GEM
RAS-like protein KIR
kinase-inducible Ras-like protein
GTP-binding mitogen-induced T-cell protein
GTP-binding protein overexpressed in skeletal muscle
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8q13-q21

Summary The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

600164


mRNA and Protein(s)

mRNA Protein Name
NM_005261 NP_005252 GTP-binding protein GEM
NM_181702 NP_859053 GTP-binding protein GEM


Homo sapiens (human) GEM NP_859053.1
Pan troglodytes (chimpanzee) GEM XP_001142383.1
Macaca mulatta (Rhesus monkey) GEM XP_001087974.1
Canis lupus familiaris (dog) GEM XP_854414.2
Bos taurus (cattle) GEM NP_001263234.1
Mus musculus (house mouse) Gem NP_034406.2
Rattus norvegicus (Norway rat) Gem NP_001100107.1
Gallus gallus (chicken) GEM NP_998744.1
Danio rerio (zebrafish) gem NP_001039314.1
Xenopus (Silurana) tropicalis (western clawed frog) gem NP_001090822.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GEM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu01836
NM_005261 Homo sapiens GTP binding protein overexpressed in skeletal muscle (GEM), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu01836
NM_181702 Homo sapiens GTP binding protein overexpressed in skeletal muscle (GEM), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu01836
Clone ID Related Accession (Same CDS sequence) NM_005261 , NM_181702
Accession Version NM_005261.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GTP-binding protein GEM
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB018783.1, BC022010.2 and AP003478.1. On Oct 6, 2009 this sequence version replaced gi:32483372. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10550.1, AL549292.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTCTGA ATAATGTCAC CATGCGCCAG GGCACTGTGG GCATGCAGCC ACAGCAGCAG 
CGCTGGAGCA TCCCAGCTGA TGGCAGGCAT CTGATGGTCC AGAAAGAGCC CCACCAGTAC
AGCCACCGCA ACCGCCATTC TGCTACCCCT GAGGACCACT GCCGCCGAAG CTGGTCCTCT
GACTCCACAG ACTCAGTCAT CTCCTCTGAG TCAGGGAACA CCTACTACCG AGTGGTGCTC
ATAGGGGAGC AGGGGGTGGG CAAGTCCACT CTGGCCAACA TCTTTGCAGG TGTGCATGAC
AGCATGGACA GCGACTGCGA GGTGCTGGGA GAAGATACAT ATGAACGAAC CCTGATGGTT
GATGGGGAAA GTGCAACGAT TATACTCCTG GATATGTGGG AAAATAAGGG GGAAAATGAA
TGGCTCCATG ACCACTGCAT GCAGGTCGGG GACGCATACC TGATTGTCTA CTCAATCACA
GACCGAGCGA GCTTCGAGAA GGCATCTGAG CTGCGAATCC AGCTCCGCAG GGCCCGGCAG
ACAGAGGACA TTCCCATAAT TTTGGTTGGC AACAAAAGTG ACTTAGTGCG GTGCCGAGAA
GTGTCTGTAT CAGAAGGGAG AGCCTGTGCA GTGGTGTTTG ACTGCAAGTT CATCGAGACC
TCTGCAGCTG TCCAGCACAA CGTGAAGGAG CTGTTTGAGG GCATTGTGCG ACAGGTGCGC
CTTCGGCGGG ACAGCAAGGA GAAGAATGAA CGGCGGCTGG CCTACCAGAA AAGGAAGGAG
AGCATGCCCA GGAAAGCCAG GCGCTTCTGG GGCAAGATCG TGGCCAAAAA CAACAAGAAT
ATGGCCTTCA AGCTCAAGTC CAAATCCTGC CATGACCTCT CTGTACTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005252.1
CDS240..1130
Misc Feature(1)117..119(+)
Misc Feature(2)465..1127(+)
Misc Feature(3)468..959(+)
Misc Feature(4)483..506(+)
Misc Feature(5)486..641(+)
Misc Feature(6)489..905(+)
Misc Feature(7)504..908(+)
Misc Feature(8)552..584(+)
Misc Feature(9)552..581(+)
Misc Feature(10)558..560(+)
Misc Feature(11)630..641(+)
Misc Feature(12)636..695(+)
Misc Feature(13)810..821(+)
Misc Feature(14)900..908(+)
Misc Feature(15)1020..1022(+)
Misc Feature(16)1035..1094(+)
Misc Feature(17)1104..1106(+)
Misc Feature(18)1107..1118(+)
Exon (1)1..230
Gene:GEM
Gene Synonym:
Exon (2)231..570
Gene:GEM
Gene Synonym:
Exon (3)571..647
Gene:GEM
Gene Synonym:
Exon (4)648..852
Gene:GEM
Gene Synonym:
Exon (5)853..2187
Gene:GEM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005261
Organism Homo sapiens (human)
Definition Homo sapiens GTP binding protein overexpressed in skeletal muscle (GEM), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005261

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTCTGA ATAATGTCAC CATGCGCCAG GGCACTGTGG GCATGCAGCC ACAGCAGCAG 
CGCTGGAGCA TCCCAGCTGA TGGCAGGCAT CTGATGGTCC AGAAAGAGCC CCACCAGTAC
AGCCACCGCA ACCGCCATTC TGCTACCCCT GAGGACCACT GCCGCCGAAG CTGGTCCTCT
GACTCCACAG ACTCAGTCAT CTCCTCTGAG TCAGGGAACA CCTACTACCG AGTGGTGCTC
ATAGGGGAGC AGGGGGTGGG CAAGTCCACT CTGGCCAACA TCTTTGCAGG TGTGCATGAC
AGCATGGACA GCGACTGCGA GGTGCTGGGA GAAGATACAT ATGAACGAAC CCTGATGGTT
GATGGGGAAA GTGCAACGAT TATACTCCTG GATATGTGGG AAAATAAGGG GGAAAATGAA
TGGCTCCATG ACCACTGCAT GCAGGTCGGG GACGCATACC TGATTGTCTA CTCAATCACA
GACCGAGCGA GCTTCGAGAA GGCATCTGAG CTGCGAATCC AGCTCCGCAG GGCCCGGCAG
ACAGAGGACA TTCCCATAAT TTTGGTTGGC AACAAAAGTG ACTTAGTGCG GTGCCGAGAA
GTGTCTGTAT CAGAAGGGAG AGCCTGTGCA GTGGTGTTTG ACTGCAAGTT CATCGAGACC
TCTGCAGCTG TCCAGCACAA CGTGAAGGAG CTGTTTGAGG GCATTGTGCG ACAGGTGCGC
CTTCGGCGGG ACAGCAAGGA GAAGAATGAA CGGCGGCTGG CCTACCAGAA AAGGAAGGAG
AGCATGCCCA GGAAAGCCAG GCGCTTCTGG GGCAAGATCG TGGCCAAAAA CAACAAGAAT
ATGGCCTTCA AGCTCAAGTC CAAATCCTGC CATGACCTCT CTGTACTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01836
Clone ID Related Accession (Same CDS sequence) NM_005261 , NM_181702
Accession Version NM_181702.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GTP-binding protein GEM
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022010.2 and AP003478.1. On Oct 6, 2009 this sequence version replaced gi:32483370. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022010.2, AK314017.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTCTGA ATAATGTCAC CATGCGCCAG GGCACTGTGG GCATGCAGCC ACAGCAGCAG 
CGCTGGAGCA TCCCAGCTGA TGGCAGGCAT CTGATGGTCC AGAAAGAGCC CCACCAGTAC
AGCCACCGCA ACCGCCATTC TGCTACCCCT GAGGACCACT GCCGCCGAAG CTGGTCCTCT
GACTCCACAG ACTCAGTCAT CTCCTCTGAG TCAGGGAACA CCTACTACCG AGTGGTGCTC
ATAGGGGAGC AGGGGGTGGG CAAGTCCACT CTGGCCAACA TCTTTGCAGG TGTGCATGAC
AGCATGGACA GCGACTGCGA GGTGCTGGGA GAAGATACAT ATGAACGAAC CCTGATGGTT
GATGGGGAAA GTGCAACGAT TATACTCCTG GATATGTGGG AAAATAAGGG GGAAAATGAA
TGGCTCCATG ACCACTGCAT GCAGGTCGGG GACGCATACC TGATTGTCTA CTCAATCACA
GACCGAGCGA GCTTCGAGAA GGCATCTGAG CTGCGAATCC AGCTCCGCAG GGCCCGGCAG
ACAGAGGACA TTCCCATAAT TTTGGTTGGC AACAAAAGTG ACTTAGTGCG GTGCCGAGAA
GTGTCTGTAT CAGAAGGGAG AGCCTGTGCA GTGGTGTTTG ACTGCAAGTT CATCGAGACC
TCTGCAGCTG TCCAGCACAA CGTGAAGGAG CTGTTTGAGG GCATTGTGCG ACAGGTGCGC
CTTCGGCGGG ACAGCAAGGA GAAGAATGAA CGGCGGCTGG CCTACCAGAA AAGGAAGGAG
AGCATGCCCA GGAAAGCCAG GCGCTTCTGG GGCAAGATCG TGGCCAAAAA CAACAAGAAT
ATGGCCTTCA AGCTCAAGTC CAAATCCTGC CATGACCTCT CTGTACTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_859053.1
CDS125..1015
Misc Feature(1)350..1012(+)
Misc Feature(2)353..844(+)
Misc Feature(3)368..391(+)
Misc Feature(4)371..526(+)
Misc Feature(5)374..790(+)
Misc Feature(6)389..793(+)
Misc Feature(7)437..469(+)
Misc Feature(8)437..466(+)
Misc Feature(9)443..445(+)
Misc Feature(10)515..526(+)
Misc Feature(11)521..580(+)
Misc Feature(12)695..706(+)
Misc Feature(13)785..793(+)
Misc Feature(14)905..907(+)
Misc Feature(15)920..979(+)
Misc Feature(16)989..991(+)
Misc Feature(17)992..1003(+)
Exon (1)1..115
Gene:GEM
Gene Synonym:
Exon (2)116..455
Gene:GEM
Gene Synonym:
Exon (3)456..532
Gene:GEM
Gene Synonym:
Exon (4)533..737
Gene:GEM
Gene Synonym:
Exon (5)738..2072
Gene:GEM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181702
Organism Homo sapiens (human)
Definition Homo sapiens GTP binding protein overexpressed in skeletal muscle (GEM), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181702

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTCTGA ATAATGTCAC CATGCGCCAG GGCACTGTGG GCATGCAGCC ACAGCAGCAG 
CGCTGGAGCA TCCCAGCTGA TGGCAGGCAT CTGATGGTCC AGAAAGAGCC CCACCAGTAC
AGCCACCGCA ACCGCCATTC TGCTACCCCT GAGGACCACT GCCGCCGAAG CTGGTCCTCT
GACTCCACAG ACTCAGTCAT CTCCTCTGAG TCAGGGAACA CCTACTACCG AGTGGTGCTC
ATAGGGGAGC AGGGGGTGGG CAAGTCCACT CTGGCCAACA TCTTTGCAGG TGTGCATGAC
AGCATGGACA GCGACTGCGA GGTGCTGGGA GAAGATACAT ATGAACGAAC CCTGATGGTT
GATGGGGAAA GTGCAACGAT TATACTCCTG GATATGTGGG AAAATAAGGG GGAAAATGAA
TGGCTCCATG ACCACTGCAT GCAGGTCGGG GACGCATACC TGATTGTCTA CTCAATCACA
GACCGAGCGA GCTTCGAGAA GGCATCTGAG CTGCGAATCC AGCTCCGCAG GGCCCGGCAG
ACAGAGGACA TTCCCATAAT TTTGGTTGGC AACAAAAGTG ACTTAGTGCG GTGCCGAGAA
GTGTCTGTAT CAGAAGGGAG AGCCTGTGCA GTGGTGTTTG ACTGCAAGTT CATCGAGACC
TCTGCAGCTG TCCAGCACAA CGTGAAGGAG CTGTTTGAGG GCATTGTGCG ACAGGTGCGC
CTTCGGCGGG ACAGCAAGGA GAAGAATGAA CGGCGGCTGG CCTACCAGAA AAGGAAGGAG
AGCATGCCCA GGAAAGCCAG GCGCTTCTGG GGCAAGATCG TGGCCAAAAA CAACAAGAAT
ATGGCCTTCA AGCTCAAGTC CAAATCCTGC CATGACCTCT CTGTACTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The GTPase Gem and its partner Kif9 are required for chromosome alignment, spindle length control, and mitotic progression
FASEB J. 26 (12), 5025-5034 (2012)
Andrieu G, Quaranta M, Leprince C and Hatzoglou A.


book

Molecular determinants of Gem protein inhibition of P/Q-type Ca2+ channels
J. Biol. Chem. 287 (27), 22749-22758 (2012)
Fan M, Zhang WK, Buraei Z and Yang J.


book

A systemic network for Chlamydia pneumoniae entry into human cells
J. Bacteriol. 192 (11), 2809-2815 (2010)
Wang A, Johnston SC, Chou J and Dean D.


book

An empirical framework for binary interactome mapping
Nat. Methods 6 (1), 83-90 (2009)
Venkatesan K, Rual JF, Vazquez A, Stelzl U, Lemmens I, Hirozane-Kishikawa T, Hao T, Zenkner M, Xin X, Goh KI, Yildirim MA, Simonis N, Heinzmann K, Gebreab F, Sahalie JM, Cevik S, Simon C, de Smet AS, Dann E, Smolyar A, Vinayagam A, Yu H, Szeto D, Borick H, Dricot A, Klitgord N, Murray RR, Lin C, Lalowski M, Timm J, Rau K, Boone C, Braun P, Cusick ME, Roth FP, Hill DE, Tavernier J, Wanker EE, Barabasi AL and Vidal M.


book

Biochemical and structural characterization of the gem GTPase
J. Biol. Chem. 282 (3), 1905-1915 (2007)
Splingard A, Menetrey J, Perderiset M, Cicolari J, Regazzoni K, Hamoudi F, Cabanie L, El Marjou A, Wells A, Houdusse A and de Gunzburg J.


book

Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
Nature 411 (6838), 701-706 (2001)
Beguin P, Nagashima K, Gonoi T, Shibasaki T, Takahashi K, Kashima Y, Ozaki N, Geering K, Iwanaga T and Seino S.


book

Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II
J. Biol. Chem. 272 (18), 11832-11839 (1997)
Moyers JS, Bilan PJ, Zhu J and Kahn CR.


book

Chromosomal organization and transcriptional regulation of human GEM and localization of the human and mouse GEM loci encoding an inducible Ras-like protein
Genomics 30 (3), 558-564 (1995)
Santoro T, Maguire J, McBride OW, Avraham KB, Copeland NG, Jenkins NA and Kelly K.


book

Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases
Proc. Natl. Acad. Sci. U.S.A. 91 (26), 12448-12452 (1994)
Cohen L, Mohr R, Chen YY, Huang M, Kato R, Dorin D, Tamanoi F, Goga A, Afar D and Rosenberg N.


book

Gem: an induced, immediate early protein belonging to the Ras family
Science 265 (5169), 241-244 (1994)
Maguire J, Santoro T, Jensen P, Siebenlist U, Yewdell J and Kelly K.


 
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