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EIF3K cDNA ORF clone, Homo sapiens (human)

Gene Symbol EIF3K
Entrez Gene ID 27335
Full Name eukaryotic translation initiation factor 3, subunit K
Synonyms ARG134, EIF3-p28, EIF3S12, HSPC029, M9, MSTP001, PLAC-24, PLAC24, PRO1474, PTD001
General protein information
Preferred Names
eukaryotic translation initiation factor 3 subunit K
Names
eukaryotic translation initiation factor 3 subunit K
eIF-3 p25
eIF-3 p28
muscle specific
muscle-specific gene M9 protein
eukaryotic translation initiation factor 3 subunit 12
eukaryotic translation initiation factor 3, subunit 12
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.2

Summary The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008]. lac of sum
Disorder MIM:

609596


mRNA and Protein(s)

mRNA Protein Name
NM_013234 NP_037366 eukaryotic translation initiation factor 3 subunit K isoform 1
NM_001300992 NP_001287921 eukaryotic translation initiation factor 3 subunit K isoform 2
NM_001308393 NP_001295322 eukaryotic translation initiation factor 3 subunit K isoform 3



Homo sapiens (human) EIF3K NP_037366.1
Pan troglodytes (chimpanzee) EIF3K XP_512639.2
Macaca mulatta (Rhesus monkey) EIF3K XP_001082764.1
Canis lupus familiaris (dog) EIF3K XP_533679.1
Bos taurus (cattle) EIF3K NP_001029661.1
Mus musculus (house mouse) Eif3k NP_082935.1
Rattus norvegicus (Norway rat) Eif3k NP_001099712.1
Gallus gallus (chicken) LOC101750374 XP_004950279.1
Danio rerio (zebrafish) eif3k NP_001017583.1
Drosophila melanogaster (fruit fly) CG10306 NP_611604.1
Caenorhabditis elegans eif-3.K NP_506241.1
Arabidopsis thaliana (thale cress) EIF3K NP_195051.1
Xenopus (Silurana) tropicalis (western clawed frog) eif3k NP_001165760.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EIF3K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF3K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30125
NM_013234 Homo sapiens eukaryotic translation initiation factor 3, subunit K (EIF3K), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu52170 NM_001300992 Homo sapiens eukaryotic translation initiation factor 3, subunit K (EIF3K), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu67213 NM_001308393 Homo sapiens eukaryotic translation initiation factor 3, subunit K (EIF3K), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30125
Clone ID Related Accession (Same CDS sequence) NM_013234
Accession Version NM_013234.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product eukaryotic translation initiation factor 3 subunit K isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BG943109.1, BC001031.2, AI054400.1 and BF965565.1. On Jul 17, 2014 this sequence version replaced gi:83656777. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF965565.1, AF085358.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGATGT TTGAGCAGAT GAGAGCCAAC GTGGGCAAGT TGCTCAAGGG TATCGACAGG 
TACAATCCTG AGAACCTGGC CACCCTGGAG CGCTATGTAG AGACGCAGGC CAAGGAAAAT
GCCTATGATC TGGAAGCCAA CCTGGCTGTC CTGAAGCTGT ACCAGTTCAA CCCAGCCTTC
TTTCAGACCA CGGTCACCGC CCAGATCCTG CTGAAGGCCC TCACCAACTT GCCGCACACA
GACTTCACCC TGTGCAAGTG CATGATCGAC CAGGCACATC AAGAAGAACG GCCAATCCGA
CAGATTTTGT ACCTCGGGGA CCTGCTGGAG ACCTGCCATT TCCAGGCCTT CTGGCAAGCC
CTGGATGAAA ACATGGACCT CTTGGAAGGT ATAACTGGCT TTGAAGACTC TGTCCGAAAG
TTTATCTGCC ATGTTGTGGG TATCACTTAC CAGCACATTG ACCGCTGGCT GCTGGCCGAG
ATGCTCGGGG ATCTGTCGGA CAGCCAGCTA AAGGTGTGGA TGAGCAAATA CGGCTGGAGT
GCCGACGAGT CGGGGCAGAT CTTCATCTGT AGCCAAGAAG AGAGCATTAA ACCCAAGAAC
ATTGTGGAGA AGATTGACTT TGACAGTGTG TCCAGCATCA TGGCCTCCTC CCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_037366.1
CDS196..852
Misc Feature(1)199..201(+)
Misc Feature(2)256..684(+)
Misc Feature(3)373..795(+)
Misc Feature(4)841..843(+)
Misc Feature(5)844..846(+)
Misc Feature(6)844..846(+)
Exon (1)1..254
Gene:EIF3K
Gene Synonym:
Exon (2)255..353
Gene:EIF3K
Gene Synonym:
Exon (3)354..474
Gene:EIF3K
Gene Synonym:
Exon (4)475..549
Gene:EIF3K
Gene Synonym:
Exon (5)550..616
Gene:EIF3K
Gene Synonym:
Exon (6)617..694
Gene:EIF3K
Gene Synonym:
Exon (7)695..820
Gene:EIF3K
Gene Synonym:
Exon (8)821..891
Gene:EIF3K
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013234
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 3, subunit K (EIF3K), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013234

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGATGT TTGAGCAGAT GAGAGCCAAC GTGGGCAAGT TGCTCAAGGG TATCGACAGG 
TACAATCCTG AGAACCTGGC CACCCTGGAG CGCTATGTAG AGACGCAGGC CAAGGAAAAT
GCCTATGATC TGGAAGCCAA CCTGGCTGTC CTGAAGCTGT ACCAGTTCAA CCCAGCCTTC
TTTCAGACCA CGGTCACCGC CCAGATCCTG CTGAAGGCCC TCACCAACTT GCCGCACACA
GACTTCACCC TGTGCAAGTG CATGATCGAC CAGGCACATC AAGAAGAACG GCCAATCCGA
CAGATTTTGT ACCTCGGGGA CCTGCTGGAG ACCTGCCATT TCCAGGCCTT CTGGCAAGCC
CTGGATGAAA ACATGGACCT CTTGGAAGGT ATAACTGGCT TTGAAGACTC TGTCCGAAAG
TTTATCTGCC ATGTTGTGGG TATCACTTAC CAGCACATTG ACCGCTGGCT GCTGGCCGAG
ATGCTCGGGG ATCTGTCGGA CAGCCAGCTA AAGGTGTGGA TGAGCAAATA CGGCTGGAGT
GCCGACGAGT CGGGGCAGAT CTTCATCTGT AGCCAAGAAG AGAGCATTAA ACCCAAGAAC
ATTGTGGAGA AGATTGACTT TGACAGTGTG TCCAGCATCA TGGCCTCCTC CCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52170
Clone ID Related Accession (Same CDS sequence) NM_001300992
Accession Version NM_001300992.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 579bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product eukaryotic translation initiation factor 3 subunit K isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG943109.1, BG470776.1, JX870647.1, AI054400.1 and BF965565.1. On Jul 17, 2014 this sequence version replaced gi:578834290. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG470776.1, BE382785.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCGATGT TTGAGCAGAT GAGAGCCAAC GTGGGCAAGT TGCTCAAGGG TATCGACAGG 
TACAATCCTG AGAACCTGGC CACCCTGGAG CGCTATGTAG AGACGCAGGC CAAGGAAAAT
GCCTATGATC TGGAAGCCAA CCTGGCTGTC CTGAAGCTGT ACCAGTTCAA CCCAGCCTTC
TTTCAGACCA CGGTCACCGC CCAGATCCTG CTGAAGGCCC TCACCAACTT GCCGCACACA
GACTTCACCC TGTGCAAGTG CATGATCGAC CAGGCACATC AAGAAGAACG GCCAATCCGA
CAGATTTTGT ACCTCGGGGA CCTGCTGGAG ACCTGCCATT TCCAGGCCTT CTGGCAAGCC
CTGGATGAAA ACATGGACCT CTTGGAAGGT ATAACTGGCT TTGAAGACTC TGTCCGAAAG
TACAGCCAGC TAAAGGTGTG GATGAGCAAA TACGGCTGGA GTGCCGACGA GTCGGGGCAG
ATCTTCATCT GTAGCCAAGA AGAGAGCATT AAACCCAAGA ACATTGTGGA GAAGATTGAC
TTTGACAGTG TGTCCAGCAT CATGGCCTCC TCCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001287921.1
CDS196..774
Misc Feature(1)256..618(+)
Misc Feature(2)373..717(+)
Exon (1)1..254
Gene:EIF3K
Gene Synonym:
Exon (2)255..353
Gene:EIF3K
Gene Synonym:
Exon (3)354..474
Gene:EIF3K
Gene Synonym:
Exon (4)475..549
Gene:EIF3K
Gene Synonym:
Exon (5)550..616
Gene:EIF3K
Gene Synonym:
Exon (6)617..742
Gene:EIF3K
Gene Synonym:
Exon (7)743..813
Gene:EIF3K
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001300992
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 3, subunit K (EIF3K), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300992

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCGATGT TTGAGCAGAT GAGAGCCAAC GTGGGCAAGT TGCTCAAGGG TATCGACAGG 
TACAATCCTG AGAACCTGGC CACCCTGGAG CGCTATGTAG AGACGCAGGC CAAGGAAAAT
GCCTATGATC TGGAAGCCAA CCTGGCTGTC CTGAAGCTGT ACCAGTTCAA CCCAGCCTTC
TTTCAGACCA CGGTCACCGC CCAGATCCTG CTGAAGGCCC TCACCAACTT GCCGCACACA
GACTTCACCC TGTGCAAGTG CATGATCGAC CAGGCACATC AAGAAGAACG GCCAATCCGA
CAGATTTTGT ACCTCGGGGA CCTGCTGGAG ACCTGCCATT TCCAGGCCTT CTGGCAAGCC
CTGGATGAAA ACATGGACCT CTTGGAAGGT ATAACTGGCT TTGAAGACTC TGTCCGAAAG
TACAGCCAGC TAAAGGTGTG GATGAGCAAA TACGGCTGGA GTGCCGACGA GTCGGGGCAG
ATCTTCATCT GTAGCCAAGA AGAGAGCATT AAACCCAAGA ACATTGTGGA GAAGATTGAC
TTTGACAGTG TGTCCAGCAT CATGGCCTCC TCCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu67213
Clone ID Related Accession (Same CDS sequence) NM_001308393
Accession Version NM_001308393.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 396bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product eukaryotic translation initiation factor 3 subunit K isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI116511.1, BG943109.1, BG488547.1, AI054400.1 and BF965565.1. On May 5, 2015 this sequence version replaced gi:768008075. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate splice site in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 3, which has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM925729.1, BG488547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGATCGACC AGGCACATCA AGAAGAACGG CCAATCCGAC AGATTTTGTA CCTCGGGGAC 
CTGCTGGAGA CCTGCCATTT CCAGGCCTTC TGGCAAGCCC TGGATGAAAA CATGGACCTC
TTGGAAGGTA TAACTGGCTT TGAAGACTCT GTCCGAAAGT TTATCTGCCA TGTTGTGGGT
ATCACTTACC AGCACATTGA CCGCTGGCTG CTGGCCGAGA TGCTCGGGGA TCTGTCGGAC
AGCCAGCTAA AGGTGTGGAT GAGCAAATAC GGCTGGAGTG CCGACGAGTC GGGGCAGATC
TTCATCTGTA GCCAAGAAGA GAGCATTAAA CCCAAGAACA TTGTGGAGAA GATTGACTTT
GACAGTGTGT CCAGCATCAT GGCCTCCTCC CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001295322.1
CDS449..844
Misc Feature(1)209..211(+)
Misc Feature(2)449..787(+)
Misc Feature(3)452..676(+)
Exon (1)1..246
Gene:EIF3K
Gene Synonym:
Exon (2)247..345
Gene:EIF3K
Gene Synonym:
Exon (3)346..466
Gene:EIF3K
Gene Synonym:
Exon (4)467..541
Gene:EIF3K
Gene Synonym:
Exon (5)542..608
Gene:EIF3K
Gene Synonym:
Exon (6)609..686
Gene:EIF3K
Gene Synonym:
Exon (7)687..812
Gene:EIF3K
Gene Synonym:
Exon (8)813..883
Gene:EIF3K
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001308393
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 3, subunit K (EIF3K), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001308393

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGATCGACC AGGCACATCA AGAAGAACGG CCAATCCGAC AGATTTTGTA CCTCGGGGAC 
CTGCTGGAGA CCTGCCATTT CCAGGCCTTC TGGCAAGCCC TGGATGAAAA CATGGACCTC
TTGGAAGGTA TAACTGGCTT TGAAGACTCT GTCCGAAAGT TTATCTGCCA TGTTGTGGGT
ATCACTTACC AGCACATTGA CCGCTGGCTG CTGGCCGAGA TGCTCGGGGA TCTGTCGGAC
AGCCAGCTAA AGGTGTGGAT GAGCAAATAC GGCTGGAGTG CCGACGAGTC GGGGCAGATC
TTCATCTGTA GCCAAGAAGA GAGCATTAAA CCCAAGAACA TTGTGGAGAA GATTGACTTT
GACAGTGTGT CCAGCATCAT GGCCTCCTCC CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The translation initiation factor 3 subunit eIF3K interacts with PML and associates with PML nuclear bodies
Exp. Cell Res. 319 (17), 2554-2565 (2013)
Salsman J, Pinder J, Tse B, Corkery D and Dellaire G.


book

Defining the membrane proteome of NK cells
J Mass Spectrom 45 (1), 1-25 (2010)
Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.


book

Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3
Proc. Natl. Acad. Sci. U.S.A. 105 (47), 18139-18144 (2008)
Zhou M, Sandercock AM, Fraser CS, Ridlova G, Stephens E, Schenauer MR, Yokoi-Fong T, Barsky D, Leary JA, Hershey JW, Doudna JA and Robinson CV.


book

eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions
J. Cell. Sci. 121 (PT 14), 2382-2393 (2008)
Lin YM, Chen YR, Lin JR, Wang WJ, Inoko A, Inagaki M, Wu YC and Chen RH.


book

Reconstitution reveals the functional core of mammalian eIF3
EMBO J. 26 (14), 3373-3383 (2007)
Masutani M, Sonenberg N, Yokoyama S and Imataka H.


book

hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes
Genomics 89 (3), 307-315 (2007)
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE and Vidal M.


book

Identification of the p28 subunit of eukaryotic initiation factor 3(eIF3k) as a new interaction partner of cyclin D3
FEBS Lett. 573 (1-3), 139-146 (2004)
Shen X, Yang Y, Liu W, Sun M, Jiang J, Zong H and Gu J.


book

Crystal structure of human eIF3k, the first structure of eIF3 subunits
J. Biol. Chem. 279 (33), 34983-34990 (2004)
Wei Z, Zhang P, Zhou Z, Cheng Z, Wan M and Gong W.


book

Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor elF3
Eur. J. Biochem. 270 (20), 4133-4139 (2003)
Mayeur GL, Fraser CS, Peiretti F, Block KL and Hershey JW.


book

Transcript-selective translational silencing by gamma interferon is directed by a novel structural element in the ceruloplasmin mRNA 3' untranslated region
Mol. Cell. Biol. 23 (5), 1509-1519 (2003)
Sampath P, Mazumder B, Seshadri V and Fox PL.


 
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