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GNAI2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GNAI2
Entrez Gene ID 2771
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Synonyms GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
General protein information
Preferred Names
guanine nucleotide-binding protein G(i) subunit alpha-2
Names
guanine nucleotide-binding protein G(i) subunit alpha-2
adenylate cyclase-inhibiting G alpha protein
GTP-binding regulatory protein Gi alpha-2 chain
guanine nucleotide-binding protein G(i), alpha-2 subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.31

Summary The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

139360

Disorder Html: Pituitary ACTH-secreting adenoma (3); Ventricular tachycardia,

mRNA and Protein(s)

mRNA Protein Name
NM_002070 NP_002061 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1
NM_001282617 NP_001269546 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3
NM_001282618 NP_001269547 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4
NM_001282619 NP_001269548 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5
NM_001282620 NP_001269549 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6
NM_001166425 NP_001159897 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2



Homo sapiens (human) GNAI2 NP_002061.1
Pan troglodytes (chimpanzee) GNAI2 XP_001167942.1
Macaca mulatta (Rhesus monkey) GNAI2 XP_001103122.1
Canis lupus familiaris (dog) GNAI2 NP_001003364.1
Bos taurus (cattle) GNAI2 NP_001094510.1
Mus musculus (house mouse) Gnai2 NP_032164.2
Rattus norvegicus (Norway rat) Gnai2 NP_112297.1
Gallus gallus (chicken) GNAI2 NP_990733.1
Danio rerio (zebrafish) gnai2b NP_956136.1
Xenopus (Silurana) tropicalis (western clawed frog) gnai2 NP_989250.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNAI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20814
NM_002070 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu14246
NM_001282617 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu14271
NM_001282618 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu14225
NM_001282619 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu14309
NM_001282620 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu13581
NM_001166425 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20814
Clone ID Related Accession (Same CDS sequence) NM_002070
Accession Version NM_002070.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037331.1, BC012138.1, AK055574.1 and U73166.1. This sequence is a reference standard in the RefSeqGene project. On Sep 14, 2013 this sequence version replaced gi:49574535. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012138.1, X04828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCGGCCG AGCGCTCTAA GATGATCGAC 
AAGAACCTGC GGGAGGACGG AGAGAAGGCG GCGCGGGAGG TGAAGTTGCT GCTGTTGGGT
GCTGGGGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA CGAGGATGGC
TACTCCGAGG AGGAATGCCG GCAGTACCGG GCGGTTGTCT ACAGCAACAC CATCCAGTCC
ATCATGGCCA TTGTCAAAGC CATGGGCAAC CTGCAGATCG ACTTTGCCGA CCCCTCCAGA
GCGGACGACG CCAGGCAGCT ATTTGCACTG TCCTGCACCG CCGAGGAGCA AGGCGTGCTC
CCTGATGACC TGTCCGGCGT CATCCGGAGG CTCTGGGCTG ACCATGGTGT GCAGGCCTGC
TTTGGCCGCT CAAGGGAATA CCAGCTCAAC GACTCAGCTG CCTACTACCT GAACGACCTG
GAGCGTATTG CACAGAGTGA CTACATCCCC ACACAGCAAG ATGTGCTACG GACCCGCGTA
AAGACCACGG GGATCGTGGA GACACACTTC ACCTTCAAGG ACCTACACTT CAAGATGTTT
GATGTGGGTG GTCAGCGGTC TGAGCGGAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACA
GCCATCATCT TCTGCGTAGC CTTGAGCGCC TATGACTTGG TGCTAGCTGA GGACGAGGAG
ATGAACCGCA TGCATGAGAG CATGAAGCTA TTCGATAGCA TCTGCAACAA CAAGTGGTTC
ACAGACACGT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAGGA GAAGATCACA
CACAGTCCCC TGACCATCTG CTTCCCTGAG TACACAGGGG CCAACAAATA TGATGAGGCA
GCCAGCTACA TCCAGAGTAA GTTTGAGGAC CTGAATAAGC GCAAAGACAC CAAGGAGATC
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTCGTGTT TGACGCCGTC
ACCGATGTCA TCATCAAGAA CAACCTGAAG GACTGCGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002061.1
CDS180..1247
Misc Feature(1)216..1238(+)
Misc Feature(2)279..1226(+)
Misc Feature(3)297..1160(+)
Misc Feature(4)297..320(+)
Misc Feature(5)300..827(+)
Misc Feature(6)384..386(+)
Misc Feature(7)708..734(+)
Misc Feature(8)723..725(+)
Misc Feature(9)726..830(+)
Misc Feature(10)732..830(+)
Misc Feature(11)780..791(+)
Misc Feature(12)783..833(+)
Misc Feature(13)987..998(+)
Misc Feature(14)1131..1181(+)
Misc Feature(15)1155..1163(+)
Exon (1)1..297
Gene:GNAI2
Gene Synonym:
Exon (2)298..340
Gene:GNAI2
Gene Synonym:
Exon (3)341..482
Gene:GNAI2
Gene Synonym:
Exon (4)483..643
Gene:GNAI2
Gene Synonym:
Exon (5)644..772
Gene:GNAI2
Gene Synonym:
Exon (6)773..902
Gene:GNAI2
Gene Synonym:
Exon (7)903..1056
Gene:GNAI2
Gene Synonym:
Exon (8)1057..1271
Gene:GNAI2
Gene Synonym:
Exon (9)1272..2258
Gene:GNAI2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002070
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002070

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCGGCCG AGCGCTCTAA GATGATCGAC 
AAGAACCTGC GGGAGGACGG AGAGAAGGCG GCGCGGGAGG TGAAGTTGCT GCTGTTGGGT
GCTGGGGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA CGAGGATGGC
TACTCCGAGG AGGAATGCCG GCAGTACCGG GCGGTTGTCT ACAGCAACAC CATCCAGTCC
ATCATGGCCA TTGTCAAAGC CATGGGCAAC CTGCAGATCG ACTTTGCCGA CCCCTCCAGA
GCGGACGACG CCAGGCAGCT ATTTGCACTG TCCTGCACCG CCGAGGAGCA AGGCGTGCTC
CCTGATGACC TGTCCGGCGT CATCCGGAGG CTCTGGGCTG ACCATGGTGT GCAGGCCTGC
TTTGGCCGCT CAAGGGAATA CCAGCTCAAC GACTCAGCTG CCTACTACCT GAACGACCTG
GAGCGTATTG CACAGAGTGA CTACATCCCC ACACAGCAAG ATGTGCTACG GACCCGCGTA
AAGACCACGG GGATCGTGGA GACACACTTC ACCTTCAAGG ACCTACACTT CAAGATGTTT
GATGTGGGTG GTCAGCGGTC TGAGCGGAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACA
GCCATCATCT TCTGCGTAGC CTTGAGCGCC TATGACTTGG TGCTAGCTGA GGACGAGGAG
ATGAACCGCA TGCATGAGAG CATGAAGCTA TTCGATAGCA TCTGCAACAA CAAGTGGTTC
ACAGACACGT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAGGA GAAGATCACA
CACAGTCCCC TGACCATCTG CTTCCCTGAG TACACAGGGG CCAACAAATA TGATGAGGCA
GCCAGCTACA TCCAGAGTAA GTTTGAGGAC CTGAATAAGC GCAAAGACAC CAAGGAGATC
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTCGTGTT TGACGCCGTC
ACCGATGTCA TCATCAAGAA CAACCTGAAG GACTGCGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14246
Clone ID Related Accession (Same CDS sequence) NM_001282617
Accession Version NM_001282617.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 912bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126708.1, AK096595.1 and AK055574.1. On Sep 14, 2013 this sequence version replaced gi:530372315. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGATCA TCCACGAGGA TGGCTACTCC GAGGAGGAAT GCCGGCAGTA CCGGGCGGTT 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATTGTCA AAGCCATGGG CAACCTGCAG
ATCGACTTTG CCGACCCCTC CAGAGCGGAC GACGCCAGGC AGCTATTTGC ACTGTCCTGC
ACCGCCGAGG AGCAAGGCGT GCTCCCTGAT GACCTGTCCG GCGTCATCCG GAGGCTCTGG
GCTGACCATG GTGTGCAGGC CTGCTTTGGC CGCTCAAGGG AATACCAGCT CAACGACTCA
GCTGCCTACT ACCTGAACGA CCTGGAGCGT ATTGCACAGA GTGACTACAT CCCCACACAG
CAAGATGTGC TACGGACCCG CGTAAAGACC ACGGGGATCG TGGAGACACA CTTCACCTTC
AAGGACCTAC ACTTCAAGAT GTTTGATGTG GGTGGTCAGC GGTCTGAGCG GAAGAAGTGG
ATCCACTGCT TTGAGGGCGT CACAGCCATC ATCTTCTGCG TAGCCTTGAG CGCCTATGAC
TTGGTGCTAG CTGAGGACGA GGAGATGAAC CGCATGCATG AGAGCATGAA GCTATTCGAT
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG
GACCTGTTTG AGGAGAAGAT CACACACAGT CCCCTGACCA TCTGCTTCCC TGAGTACACA
GGGGCCAACA AATATGATGA GGCAGCCAGC TACATCCAGA GTAAGTTTGA GGACCTGAAT
AAGCGCAAAG ACACCAAGGA GATCTACACG CACTTCACGT GCGCCACCGA CACCAAGAAC
GTGCAGTTCG TGTTTGACGC CGTCACCGAT GTCATCATCA AGAACAACCT GAAGGACTGC
GGCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269546.1
CDS262..1173
Misc Feature(1)202..204(+)
Misc Feature(2)262..1152(+)
Misc Feature(3)262..1137(+)
Misc Feature(4)316..753(+)
Misc Feature(5)634..660(+)
Misc Feature(6)649..1086(+)
Misc Feature(7)649..651(+)
Misc Feature(8)652..756(+)
Misc Feature(9)658..756(+)
Misc Feature(10)706..717(+)
Misc Feature(11)709..759(+)
Misc Feature(12)913..924(+)
Misc Feature(13)1057..1107(+)
Misc Feature(14)1081..1089(+)
Exon (1)1..223
Gene:GNAI2
Gene Synonym:
Exon (2)224..266
Gene:GNAI2
Gene Synonym:
Exon (3)267..408
Gene:GNAI2
Gene Synonym:
Exon (4)409..569
Gene:GNAI2
Gene Synonym:
Exon (5)570..698
Gene:GNAI2
Gene Synonym:
Exon (6)699..828
Gene:GNAI2
Gene Synonym:
Exon (7)829..982
Gene:GNAI2
Gene Synonym:
Exon (8)983..1197
Gene:GNAI2
Gene Synonym:
Exon (9)1198..2184
Gene:GNAI2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282617
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282617

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGATCA TCCACGAGGA TGGCTACTCC GAGGAGGAAT GCCGGCAGTA CCGGGCGGTT 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATTGTCA AAGCCATGGG CAACCTGCAG
ATCGACTTTG CCGACCCCTC CAGAGCGGAC GACGCCAGGC AGCTATTTGC ACTGTCCTGC
ACCGCCGAGG AGCAAGGCGT GCTCCCTGAT GACCTGTCCG GCGTCATCCG GAGGCTCTGG
GCTGACCATG GTGTGCAGGC CTGCTTTGGC CGCTCAAGGG AATACCAGCT CAACGACTCA
GCTGCCTACT ACCTGAACGA CCTGGAGCGT ATTGCACAGA GTGACTACAT CCCCACACAG
CAAGATGTGC TACGGACCCG CGTAAAGACC ACGGGGATCG TGGAGACACA CTTCACCTTC
AAGGACCTAC ACTTCAAGAT GTTTGATGTG GGTGGTCAGC GGTCTGAGCG GAAGAAGTGG
ATCCACTGCT TTGAGGGCGT CACAGCCATC ATCTTCTGCG TAGCCTTGAG CGCCTATGAC
TTGGTGCTAG CTGAGGACGA GGAGATGAAC CGCATGCATG AGAGCATGAA GCTATTCGAT
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG
GACCTGTTTG AGGAGAAGAT CACACACAGT CCCCTGACCA TCTGCTTCCC TGAGTACACA
GGGGCCAACA AATATGATGA GGCAGCCAGC TACATCCAGA GTAAGTTTGA GGACCTGAAT
AAGCGCAAAG ACACCAAGGA GATCTACACG CACTTCACGT GCGCCACCGA CACCAAGAAC
GTGCAGTTCG TGTTTGACGC CGTCACCGAT GTCATCATCA AGAACAACCT GAAGGACTGC
GGCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14271
Clone ID Related Accession (Same CDS sequence) NM_001282618
Accession Version NM_001282618.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037331.1, BC012138.1, AK055574.1 and AK096595.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCATTG TCAAAGCCAT GGGCAACCTG CAGATCGACT TTGCCGACCC CTCCAGAGCG 
GACGACGCCA GGCAGCTATT TGCACTGTCC TGCACCGCCG AGGAGCAAGG CGTGCTCCCT
GATGACCTGT CCGGCGTCAT CCGGAGGCTC TGGGCTGACC ATGGTGTGCA GGCCTGCTTT
GGCCGCTCAA GGGAATACCA GCTCAACGAC TCAGCTGCCT ACTACCTGAA CGACCTGGAG
CGTATTGCAC AGAGTGACTA CATCCCCACA CAGCAAGATG TGCTACGGAC CCGCGTAAAG
ACCACGGGGA TCGTGGAGAC ACACTTCACC TTCAAGGACC TACACTTCAA GATGTTTGAT
GTGGGTGGTC AGCGGTCTGA GCGGAAGAAG TGGATCCACT GCTTTGAGGG CGTCACAGCC
ATCATCTTCT GCGTAGCCTT GAGCGCCTAT GACTTGGTGC TAGCTGAGGA CGAGGAGATG
AACCGCATGC ATGAGAGCAT GAAGCTATTC GATAGCATCT GCAACAACAA GTGGTTCACA
GACACGTCCA TCATCCTCTT CCTCAACAAG AAGGACCTGT TTGAGGAGAA GATCACACAC
AGTCCCCTGA CCATCTGCTT CCCTGAGTAC ACAGGGGCCA ACAAATATGA TGAGGCAGCC
AGCTACATCC AGAGTAAGTT TGAGGACCTG AATAAGCGCA AAGACACCAA GGAGATCTAC
ACGCACTTCA CGTGCGCCAC CGACACCAAG AACGTGCAGT TCGTGTTTGA CGCCGTCACC
GATGTCATCA TCAAGAACAA CCTGAAGGAC TGCGGCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269547.1
CDS380..1204
Misc Feature(1)227..229(+)
Misc Feature(2)383..1195(+)
Misc Feature(3)383..1183(+)
Misc Feature(4)389..784(+)
Misc Feature(5)665..691(+)
Misc Feature(6)680..1117(+)
Misc Feature(7)680..682(+)
Misc Feature(8)683..787(+)
Misc Feature(9)689..787(+)
Misc Feature(10)737..748(+)
Misc Feature(11)740..790(+)
Misc Feature(12)944..955(+)
Misc Feature(13)1088..1138(+)
Misc Feature(14)1112..1120(+)
Exon (1)1..297
Gene:GNAI2
Gene Synonym:
Exon (2)298..439
Gene:GNAI2
Gene Synonym:
Exon (3)440..600
Gene:GNAI2
Gene Synonym:
Exon (4)601..729
Gene:GNAI2
Gene Synonym:
Exon (5)730..859
Gene:GNAI2
Gene Synonym:
Exon (6)860..1013
Gene:GNAI2
Gene Synonym:
Exon (7)1014..1228
Gene:GNAI2
Gene Synonym:
Exon (8)1229..2215
Gene:GNAI2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282618
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282618

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCATTG TCAAAGCCAT GGGCAACCTG CAGATCGACT TTGCCGACCC CTCCAGAGCG 
GACGACGCCA GGCAGCTATT TGCACTGTCC TGCACCGCCG AGGAGCAAGG CGTGCTCCCT
GATGACCTGT CCGGCGTCAT CCGGAGGCTC TGGGCTGACC ATGGTGTGCA GGCCTGCTTT
GGCCGCTCAA GGGAATACCA GCTCAACGAC TCAGCTGCCT ACTACCTGAA CGACCTGGAG
CGTATTGCAC AGAGTGACTA CATCCCCACA CAGCAAGATG TGCTACGGAC CCGCGTAAAG
ACCACGGGGA TCGTGGAGAC ACACTTCACC TTCAAGGACC TACACTTCAA GATGTTTGAT
GTGGGTGGTC AGCGGTCTGA GCGGAAGAAG TGGATCCACT GCTTTGAGGG CGTCACAGCC
ATCATCTTCT GCGTAGCCTT GAGCGCCTAT GACTTGGTGC TAGCTGAGGA CGAGGAGATG
AACCGCATGC ATGAGAGCAT GAAGCTATTC GATAGCATCT GCAACAACAA GTGGTTCACA
GACACGTCCA TCATCCTCTT CCTCAACAAG AAGGACCTGT TTGAGGAGAA GATCACACAC
AGTCCCCTGA CCATCTGCTT CCCTGAGTAC ACAGGGGCCA ACAAATATGA TGAGGCAGCC
AGCTACATCC AGAGTAAGTT TGAGGACCTG AATAAGCGCA AAGACACCAA GGAGATCTAC
ACGCACTTCA CGTGCGCCAC CGACACCAAG AACGTGCAGT TCGTGTTTGA CGCCGTCACC
GATGTCATCA TCAAGAACAA CCTGAAGGAC TGCGGCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14225
Clone ID Related Accession (Same CDS sequence) NM_001282619
Accession Version NM_001282619.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA528337.1, BC016995.1 and AK055574.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016995.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGTATG CAGGGCATCT TCCTGCCAGC TCTGCCCAGG GCACCATATT GGCATGCACC 
AGCTGCACAG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269548.1
CDS561..1580
Misc Feature(1)519..521(+)
Misc Feature(2)630..1571(+)
Misc Feature(3)630..1559(+)
Misc Feature(4)630..1493(+)
Misc Feature(5)630..653(+)
Misc Feature(6)633..1160(+)
Misc Feature(7)1041..1067(+)
Misc Feature(8)1056..1058(+)
Misc Feature(9)1059..1163(+)
Misc Feature(10)1065..1163(+)
Misc Feature(11)1113..1124(+)
Misc Feature(12)1116..1166(+)
Misc Feature(13)1320..1331(+)
Misc Feature(14)1464..1514(+)
Misc Feature(15)1488..1496(+)
Exon (1)1..506
Gene:GNAI2
Gene Synonym:
Exon (2)507..630
Gene:GNAI2
Gene Synonym:
Exon (3)631..673
Gene:GNAI2
Gene Synonym:
Exon (4)674..815
Gene:GNAI2
Gene Synonym:
Exon (5)816..976
Gene:GNAI2
Gene Synonym:
Exon (6)977..1105
Gene:GNAI2
Gene Synonym:
Exon (7)1106..1235
Gene:GNAI2
Gene Synonym:
Exon (8)1236..1389
Gene:GNAI2
Gene Synonym:
Exon (9)1390..1604
Gene:GNAI2
Gene Synonym:
Exon (10)1605..2591
Gene:GNAI2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282619
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGTATG CAGGGCATCT TCCTGCCAGC TCTGCCCAGG GCACCATATT GGCATGCACC 
AGCTGCACAG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14309
Clone ID Related Accession (Same CDS sequence) NM_001282620
Accession Version NM_001282620.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA528337.1, AK096595.1 and AK055574.1. On Sep 14, 2013 this sequence version replaced gi:530372311. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (6) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096595.1, AK096299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACGGAGG GTGTGAAGAC GCTAGGCTGG ACGAAGCAGA AAGGCGGGTG TCACTGGGGA 
CGTTCTGAGG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269549.1
CDS437..1456
Misc Feature(1)404..406(+)
Misc Feature(2)506..1447(+)
Misc Feature(3)506..1435(+)
Misc Feature(4)506..1369(+)
Misc Feature(5)506..529(+)
Misc Feature(6)509..1036(+)
Misc Feature(7)917..943(+)
Misc Feature(8)932..934(+)
Misc Feature(9)935..1039(+)
Misc Feature(10)941..1039(+)
Misc Feature(11)989..1000(+)
Misc Feature(12)992..1042(+)
Misc Feature(13)1196..1207(+)
Misc Feature(14)1340..1390(+)
Misc Feature(15)1364..1372(+)
Exon (1)1..506
Gene:GNAI2
Gene Synonym:
Exon (2)507..549
Gene:GNAI2
Gene Synonym:
Exon (3)550..691
Gene:GNAI2
Gene Synonym:
Exon (4)692..852
Gene:GNAI2
Gene Synonym:
Exon (5)853..981
Gene:GNAI2
Gene Synonym:
Exon (6)982..1111
Gene:GNAI2
Gene Synonym:
Exon (7)1112..1265
Gene:GNAI2
Gene Synonym:
Exon (8)1266..1480
Gene:GNAI2
Gene Synonym:
Exon (9)1481..2467
Gene:GNAI2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282620
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282620

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACGGAGG GTGTGAAGAC GCTAGGCTGG ACGAAGCAGA AAGGCGGGTG TCACTGGGGA 
CGTTCTGAGG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13581
Clone ID Related Accession (Same CDS sequence) NM_001166425
Accession Version NM_001166425.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB044421.1, AK302330.1, AK126708.1 and AK055574.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGAGGTG CTGGGGAGTC AGGGAAGAGC ACCATCGTCA AGCAGATGAA GATCATCCAC 
GAGGATGGCT ACTCCGAGGA GGAATGCCGG CAGTACCGGG CGGTTGTCTA CAGCAACACC
ATCCAGTCCA TCATGGCCAT TGTCAAAGCC ATGGGCAACC TGCAGATCGA CTTTGCCGAC
CCCTCCAGAG CGGACGACGC CAGGCAGCTA TTTGCACTGT CCTGCACCGC CGAGGAGCAA
GGCGTGCTCC CTGATGACCT GTCCGGCGTC ATCCGGAGGC TCTGGGCTGA CCATGGTGTG
CAGGCCTGCT TTGGCCGCTC AAGGGAATAC CAGCTCAACG ACTCAGCTGC CTACTACCTG
AACGACCTGG AGCGTATTGC ACAGAGTGAC TACATCCCCA CACAGCAAGA TGTGCTACGG
ACCCGCGTAA AGACCACGGG GATCGTGGAG ACACACTTCA CCTTCAAGGA CCTACACTTC
AAGATGTTTG ATGTGGGTGG TCAGCGGTCT GAGCGGAAGA AGTGGATCCA CTGCTTTGAG
GGCGTCACAG CCATCATCTT CTGCGTAGCC TTGAGCGCCT ATGACTTGGT GCTAGCTGAG
GACGAGGAGA TGAACCGCAT GCATGAGAGC ATGAAGCTAT TCGATAGCAT CTGCAACAAC
AAGTGGTTCA CAGACACGTC CATCATCCTC TTCCTCAACA AGAAGGACCT GTTTGAGGAG
AAGATCACAC ACAGTCCCCT GACCATCTGC TTCCCTGAGT ACACAGGGGC CAACAAATAT
GATGAGGCAG CCAGCTACAT CCAGAGTAAG TTTGAGGACC TGAATAAGCG CAAAGACACC
AAGGAGATCT ACACGCACTT CACGTGCGCC ACCGACACCA AGAACGTGCA GTTCGTGTTT
GACGCCGTCA CCGATGTCAT CATCAAGAAC AACCTGAAGG ACTGCGGCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159897.1
CDS176..1132
Misc Feature(1)137..139(+)
Misc Feature(2)182..1123(+)
Misc Feature(3)182..1111(+)
Misc Feature(4)182..1045(+)
Misc Feature(5)182..205(+)
Misc Feature(6)185..712(+)
Misc Feature(7)593..619(+)
Misc Feature(8)608..610(+)
Misc Feature(9)611..715(+)
Misc Feature(10)617..715(+)
Misc Feature(11)665..676(+)
Misc Feature(12)668..718(+)
Misc Feature(13)872..883(+)
Misc Feature(14)1016..1066(+)
Misc Feature(15)1040..1048(+)
Exon (1)1..182
Gene:GNAI2
Gene Synonym:
Exon (2)183..225
Gene:GNAI2
Gene Synonym:
Exon (3)226..367
Gene:GNAI2
Gene Synonym:
Exon (4)368..528
Gene:GNAI2
Gene Synonym:
Exon (5)529..657
Gene:GNAI2
Gene Synonym:
Exon (6)658..787
Gene:GNAI2
Gene Synonym:
Exon (7)788..941
Gene:GNAI2
Gene Synonym:
Exon (8)942..1156
Gene:GNAI2
Gene Synonym:
Exon (9)1157..2143
Gene:GNAI2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166425
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166425

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGAGGTG CTGGGGAGTC AGGGAAGAGC ACCATCGTCA AGCAGATGAA GATCATCCAC 
GAGGATGGCT ACTCCGAGGA GGAATGCCGG CAGTACCGGG CGGTTGTCTA CAGCAACACC
ATCCAGTCCA TCATGGCCAT TGTCAAAGCC ATGGGCAACC TGCAGATCGA CTTTGCCGAC
CCCTCCAGAG CGGACGACGC CAGGCAGCTA TTTGCACTGT CCTGCACCGC CGAGGAGCAA
GGCGTGCTCC CTGATGACCT GTCCGGCGTC ATCCGGAGGC TCTGGGCTGA CCATGGTGTG
CAGGCCTGCT TTGGCCGCTC AAGGGAATAC CAGCTCAACG ACTCAGCTGC CTACTACCTG
AACGACCTGG AGCGTATTGC ACAGAGTGAC TACATCCCCA CACAGCAAGA TGTGCTACGG
ACCCGCGTAA AGACCACGGG GATCGTGGAG ACACACTTCA CCTTCAAGGA CCTACACTTC
AAGATGTTTG ATGTGGGTGG TCAGCGGTCT GAGCGGAAGA AGTGGATCCA CTGCTTTGAG
GGCGTCACAG CCATCATCTT CTGCGTAGCC TTGAGCGCCT ATGACTTGGT GCTAGCTGAG
GACGAGGAGA TGAACCGCAT GCATGAGAGC ATGAAGCTAT TCGATAGCAT CTGCAACAAC
AAGTGGTTCA CAGACACGTC CATCATCCTC TTCCTCAACA AGAAGGACCT GTTTGAGGAG
AAGATCACAC ACAGTCCCCT GACCATCTGC TTCCCTGAGT ACACAGGGGC CAACAAATAT
GATGAGGCAG CCAGCTACAT CCAGAGTAAG TTTGAGGACC TGAATAAGCG CAAAGACACC
AAGGAGATCT ACACGCACTT CACGTGCGCC ACCGACACCA AGAACGTGCA GTTCGTGTTT
GACGCCGTCA CCGATGTCAT CATCAAGAAC AACCTGAAGG ACTGCGGCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Galphai2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation
Mol. Cell. Biol. 34 (4), 619-630 (2014)
Minetti GC, Feige JN, Bombard F, Heier A, Morvan F, Nurnberg B, Leiss V, Birnbaumer L, Glass DJ and Fornaro M.


book

Ubiquitination of the heterotrimeric G protein alpha subunits Galphai2 and Galphaq is prevented by the guanine nucleotide exchange factor Ric-8A
Biochem. Biophys. Res. Commun. 435 (3), 414-419 (2013)
Chishiki K, Kamakura S, Yuzawa S, Hayase J and Sumimoto H.


book

Increased Gsalpha within blood cell membrane lipid microdomains in some depressive disorders: an exploratory study
J Psychiatr Res 47 (6), 706-711 (2013)
Mooney JJ, Samson JA, McHale NL, Pappalarado KM, Alpert JE and Schildkraut JJ.


book

The familial hemiplegic migraine type 1 mutation K1336E affects direct G protein-mediated regulation of neuronal P/Q-type Ca2+ channels
Cephalalgia 33 (6), 398-407 (2013)
Garza-Lopez E, Gonzalez-Ramirez R, Gandini MA, Sandoval A and Felix R.


book

HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Galpha(i2) for degradation through ubiquitination
J. Biol. Chem. 287 (49), 41481-41498 (2012)
Chandrasekaran P, Buckley M, Moore V, Wang LQ, Kehrl JH and Venkatesan S.


book

Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12p12-p13
Genomics 12 (1), 125-129 (1992)
Magovcevic I, Ang SL, Seidman JG, Tolman CJ, Neer EJ and Morton CC.


book

Identification of G protein alpha-subunits in RINm5F cells and their selective interaction with galanin receptor
Diabetes 40 (9), 1170-1176 (1991)
Cormont M, Le Marchand-Brustel Y, Van Obberghen E, Spiegel AM and Sharp GW.


book

Evidence for opioid receptor-mediated activation of the G-proteins, Go and Gi2, in membranes of neuroblastoma x glioma (NG108-15) hybrid cells
J. Biol. Chem. 266 (6), 3365-3368 (1991)
Offermanns S, Schultz G and Rosenthal W.


book

Two G protein oncogenes in human endocrine tumors
Science 249 (4969), 655-659 (1990)
Lyons J, Landis CA, Harsh G, Vallar L, Grunewald K, Feichtinger H, Duh QY, Clark OH, Kawasaki E and Bourne HR.


book

Hormonal regulation of Gi2 alpha-subunit phosphorylation in intact hepatocytes
Biochem. J. 268 (2), 449-457 (1990)
Bushfield M, Murphy GJ, Lavan BE, Parker PJ, Hruby VJ, Milligan G and Houslay MD.


 
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