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GNAI2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GNAI2
Entrez Gene ID 2771
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Synonyms GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
General protein information
Preferred Names
guanine nucleotide-binding protein G(i) subunit alpha-2
Names
guanine nucleotide-binding protein G(i) subunit alpha-2
adenylate cyclase-inhibiting G alpha protein
GTP-binding regulatory protein Gi alpha-2 chain
guanine nucleotide-binding protein G(i), alpha-2 subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p21.31

Summary The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

139360

Disorder Html: Pituitary ACTH-secreting adenoma (3); Ventricular tachycardia,

mRNA and Protein(s)

mRNA Protein Name
NM_002070 NP_002061 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1
NM_001282617 NP_001269546 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3
NM_001282618 NP_001269547 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4
NM_001282619 NP_001269548 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5
NM_001282620 NP_001269549 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6
NM_001166425 NP_001159897 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2

KEGG
hsa04360 Axon guidance
hsa04530 Tight junction
hsa04670 Leukocyte transendothelial migration
hsa04540 Gap junction
hsa04730 Long-term depression
hsa04916 Melanogenesis
hsa05012 Parkinson's disease
hsa05200 Pathways in cancer
hsa04914 Progesterone-mediated oocyte maturation
hsa04062 Chemokine signaling pathway
hsa05142 Chagas disease (American trypanosomiasis)
hsa05145 Toxoplasmosis
hsa04971 Gastric acid secretion
hsa04725 Cholinergic synapse
hsa05133 Pertussis
hsa04724 Glutamatergic synapse
hsa04727 GABAergic synapse
hsa04728 Dopaminergic synapse
hsa04726 Serotonergic synapse
hsa04723 Retrograde endocannabinoid signaling
hsa05030 Cocaine addiction
hsa05032 Morphine addiction
hsa05034 Alcoholism
hsa04713 Circadian entrainment
hsa04915 Estrogen signaling pathway
hsa04261 Adrenergic signaling in cardiomyocytes
hsa04611 Platelet activation
hsa04921 Oxytocin signaling pathway
hsa04022 cGMP-PKG signaling pathway
hsa04024 cAMP signaling pathway
hsa04071 Sphingolipid signaling pathway
hsa04924 Renin secretion
hsa04923 Regulation of lipolysis in adipocytes
hsa04015 Rap1 signaling pathway
Reactome
R-HSA-112316 Neuronal System
R-HSA-112315 Transmission across Chemical Synapses
R-HSA-112314 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
R-HSA-977444 GABA B receptor activation
R-HSA-991365 Activation of GABAB receptors
R-HSA-977443 GABA receptor activation
R-HSA-997269 Inhibition of adenylate cyclase pathway
R-HSA-170670 Adenylate cyclase inhibitory pathway
R-HSA-109582 Hemostasis
R-HSA-162582 Signal Transduction
R-HSA-392518 Signal amplification
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-392170 ADP signalling through P2Y purinoceptor 12
R-HSA-388396 GPCR downstream signaling
R-HSA-418594 G alpha (i) signalling events
R-HSA-372790 Signaling by GPCR
R-HSA-418597 G alpha (z) signalling events
R-HSA-111885 Opioid Signalling
R-HSA-418555 G alpha (s) signalling events
R-HSA-112043 PLC beta mediated events
R-HSA-202040 G-protein activation
R-HSA-112040 G-protein mediated events
R-HSA-1430728 Metabolism
R-HSA-163685 Integration of energy metabolism
R-HSA-422356 Regulation of insulin secretion
R-HSA-400042 Adrenaline,noradrenaline inhibits insulin secretion
Pathway Interaction Database
s1p_meta_pathway Sphingosine 1-phosphate (S1P) pathway
lysophospholipid_pathway LPA receptor mediated events
s1p_s1p1_pathway S1P1 pathway
cxcr3pathway CXCR3-mediated signaling events
txa2pathway Thromboxane A2 receptor signaling
s1p_s1p5_pathway S1P5 pathway
s1p_s1p2_pathway S1P2 pathway
s1p_s1p3_pathway S1P3 pathway
s1p_s1p4_pathway S1P4 pathway
WikiPathways
WP26 Signal Transduction of S1P Receptor
WP35 G Protein Signaling Pathways
WP536 Calcium Regulation in the Cardiac Cell
WP2032 TSH signaling pathway
WP2355 Corticotropin-releasing hormone


Homo sapiens (human) GNAI2 NP_002061.1
Pan troglodytes (chimpanzee) GNAI2 XP_001167942.1
Macaca mulatta (Rhesus monkey) GNAI2 XP_001103122.1
Canis lupus familiaris (dog) GNAI2 NP_001003364.1
Bos taurus (cattle) GNAI2 NP_001094510.1
Mus musculus (house mouse) Gnai2 NP_032164.2
Rattus norvegicus (Norway rat) Gnai2 NP_112297.1
Gallus gallus (chicken) GNAI2 NP_990733.1
Danio rerio (zebrafish) gnai2b NP_956136.1
Xenopus (Silurana) tropicalis (western clawed frog) gnai2 NP_989250.1

Component

ID Name Evidence
GO:0005624 membrane fraction IEA
GO:0005634 nucleus IEA
GO:0005737 cytoplasm IDA
GO:0005813 centrosome IDA
GO:0005829 cytosol IEA
GO:0005834 heterotrimeric G-protein complex ISS
GO:0005886 plasma membrane EXP
GO:0005886 plasma membrane IDA
GO:0005886 plasma membrane TAS
GO:0030496 midbody IDA
GO:0031234 extrinsic to internal side of plasma membrane ISS
GO:0045121 membrane raft IEA

Function

ID Name Evidence
GO:0000166 nucleotide binding IEA
GO:0001664 G-protein-coupled receptor binding ISS
GO:0003924 GTPase activity ISS
GO:0003924 GTPase activity TAS
GO:0004871 signal transducer activity ISS
GO:0005515 protein binding IPI
GO:0005525 GTP binding EXP
GO:0019001 guanyl nucleotide binding IEA
GO:0031683 G-protein beta/gamma-subunit complex binding ISS

Process

ID Name Evidence
GO:0000186 activation of MAPKK activity IEA
GO:0001973 adenosine receptor signaling pathway ISS
GO:0006184 GTP catabolic process ISS
GO:0006184 GTP catabolic process TAS
GO:0007165 signal transduction TAS
GO:0007186 G-protein coupled receptor protein signaling pathway TAS
GO:0007193 inhibition of adenylate cyclase activity by G-protein signaling pathway EXP
GO:0007194 negative regulation of adenylate cyclase activity TAS
GO:0007213 muscarinic acetylcholine receptor signaling pathway IEA
GO:0007214 gamma-aminobutyric acid signaling pathway ISS
GO:0007243 intracellular protein kinase cascade IEA
GO:0007268 synaptic transmission TAS
GO:0007584 response to nutrient TAS
GO:0007596 blood coagulation TAS
GO:0008284 positive regulation of cell proliferation IEA
GO:0030168 platelet activation TAS
GO:0050805 negative regulation of synaptic transmission IEA
GO:0051301 cell division IMP
GO:0051927 negative regulation of calcium ion transport via voltage-gated calcium channel activity ISS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNAI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20814
NM_002070 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $189.50
$379.00
OHu14246
NM_001282617 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $99.50
$199.00
OHu14271
NM_001282618 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $99.50
$199.00
OHu14225
NM_001282619 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $159.50
$319.00
OHu14309
NM_001282620 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $159.50
$319.00
OHu13581
NM_001166425 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $154.50
$309.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu20814
Accession Version NM_002070.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037331.1, BC012138.1, AK055574.1 and U73166.1. This sequence is a reference standard in the RefSeqGene project. On Sep 14, 2013 this sequence version replaced gi:49574535. Summary: The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012138.1, X04828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCGGCCG AGCGCTCTAA GATGATCGAC 
AAGAACCTGC GGGAGGACGG AGAGAAGGCG GCGCGGGAGG TGAAGTTGCT GCTGTTGGGT
GCTGGGGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA CGAGGATGGC
TACTCCGAGG AGGAATGCCG GCAGTACCGG GCGGTTGTCT ACAGCAACAC CATCCAGTCC
ATCATGGCCA TTGTCAAAGC CATGGGCAAC CTGCAGATCG ACTTTGCCGA CCCCTCCAGA
GCGGACGACG CCAGGCAGCT ATTTGCACTG TCCTGCACCG CCGAGGAGCA AGGCGTGCTC
CCTGATGACC TGTCCGGCGT CATCCGGAGG CTCTGGGCTG ACCATGGTGT GCAGGCCTGC
TTTGGCCGCT CAAGGGAATA CCAGCTCAAC GACTCAGCTG CCTACTACCT GAACGACCTG
GAGCGTATTG CACAGAGTGA CTACATCCCC ACACAGCAAG ATGTGCTACG GACCCGCGTA
AAGACCACGG GGATCGTGGA GACACACTTC ACCTTCAAGG ACCTACACTT CAAGATGTTT
GATGTGGGTG GTCAGCGGTC TGAGCGGAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACA
GCCATCATCT TCTGCGTAGC CTTGAGCGCC TATGACTTGG TGCTAGCTGA GGACGAGGAG
ATGAACCGCA TGCATGAGAG CATGAAGCTA TTCGATAGCA TCTGCAACAA CAAGTGGTTC
ACAGACACGT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAGGA GAAGATCACA
CACAGTCCCC TGACCATCTG CTTCCCTGAG TACACAGGGG CCAACAAATA TGATGAGGCA
GCCAGCTACA TCCAGAGTAA GTTTGAGGAC CTGAATAAGC GCAAAGACAC CAAGGAGATC
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTCGTGTT TGACGCCGTC
ACCGATGTCA TCATCAAGAA CAACCTGAAG GACTGCGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002061.1
CDS180..1247
Misc Feature(1)216..1238(+)
Misc Feature(2)279..1226(+)
Misc Feature(3)297..1160(+)
Misc Feature(4)297..320(+)
Misc Feature(5)300..827(+)
Misc Feature(6)384..386(+)
Misc Feature(7)708..734(+)
Misc Feature(8)723..725(+)
Misc Feature(9)726..830(+)
Misc Feature(10)732..830(+)
Misc Feature(11)780..791(+)
Misc Feature(12)783..833(+)
Misc Feature(13)987..998(+)
Misc Feature(14)1131..1181(+)
Misc Feature(15)1155..1163(+)
Exon (1)1..297
Gene:GNAI2
Gene Synonym:
Exon (2)298..340
Gene:GNAI2
Gene Synonym:
Exon (3)341..482
Gene:GNAI2
Gene Synonym:
Exon (4)483..643
Gene:GNAI2
Gene Synonym:
Exon (5)644..772
Gene:GNAI2
Gene Synonym:
Exon (6)773..902
Gene:GNAI2
Gene Synonym:
Exon (7)903..1056
Gene:GNAI2
Gene Synonym:
Exon (8)1057..1271
Gene:GNAI2
Gene Synonym:
Exon (9)1272..2258
Gene:GNAI2
Gene Synonym:
Translation
Position Chain Variation Link
583 583 a, g dbSNP:770199226
653 653 c, t dbSNP:199645073
714 714 c, g, t dbSNP:137853226
715 715 a, g dbSNP:137853227
779 779 a, t dbSNP:137853228
894 894 a, g dbSNP:760792214
905 905 c, t dbSNP:267599879
1029 1029 c, t dbSNP:142945610
1442 1442 g, t dbSNP:781177917
1802 1802 a, g dbSNP:76340684
1940 1940 a, g dbSNP:745422685
2249 2249 c, g dbSNP:755622250

Target ORF information:

RefSeq Version NM_002070
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002070

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCGGCCG AGCGCTCTAA GATGATCGAC 
AAGAACCTGC GGGAGGACGG AGAGAAGGCG GCGCGGGAGG TGAAGTTGCT GCTGTTGGGT
GCTGGGGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA CGAGGATGGC
TACTCCGAGG AGGAATGCCG GCAGTACCGG GCGGTTGTCT ACAGCAACAC CATCCAGTCC
ATCATGGCCA TTGTCAAAGC CATGGGCAAC CTGCAGATCG ACTTTGCCGA CCCCTCCAGA
GCGGACGACG CCAGGCAGCT ATTTGCACTG TCCTGCACCG CCGAGGAGCA AGGCGTGCTC
CCTGATGACC TGTCCGGCGT CATCCGGAGG CTCTGGGCTG ACCATGGTGT GCAGGCCTGC
TTTGGCCGCT CAAGGGAATA CCAGCTCAAC GACTCAGCTG CCTACTACCT GAACGACCTG
GAGCGTATTG CACAGAGTGA CTACATCCCC ACACAGCAAG ATGTGCTACG GACCCGCGTA
AAGACCACGG GGATCGTGGA GACACACTTC ACCTTCAAGG ACCTACACTT CAAGATGTTT
GATGTGGGTG GTCAGCGGTC TGAGCGGAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACA
GCCATCATCT TCTGCGTAGC CTTGAGCGCC TATGACTTGG TGCTAGCTGA GGACGAGGAG
ATGAACCGCA TGCATGAGAG CATGAAGCTA TTCGATAGCA TCTGCAACAA CAAGTGGTTC
ACAGACACGT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAGGA GAAGATCACA
CACAGTCCCC TGACCATCTG CTTCCCTGAG TACACAGGGG CCAACAAATA TGATGAGGCA
GCCAGCTACA TCCAGAGTAA GTTTGAGGAC CTGAATAAGC GCAAAGACAC CAAGGAGATC
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTCGTGTT TGACGCCGTC
ACCGATGTCA TCATCAAGAA CAACCTGAAG GACTGCGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14246
Accession Version NM_001282617.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 912bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126708.1, AK096595.1 and AK055574.1. On Sep 14, 2013 this sequence version replaced gi:530372315. Summary: The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGATCA TCCACGAGGA TGGCTACTCC GAGGAGGAAT GCCGGCAGTA CCGGGCGGTT 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATTGTCA AAGCCATGGG CAACCTGCAG
ATCGACTTTG CCGACCCCTC CAGAGCGGAC GACGCCAGGC AGCTATTTGC ACTGTCCTGC
ACCGCCGAGG AGCAAGGCGT GCTCCCTGAT GACCTGTCCG GCGTCATCCG GAGGCTCTGG
GCTGACCATG GTGTGCAGGC CTGCTTTGGC CGCTCAAGGG AATACCAGCT CAACGACTCA
GCTGCCTACT ACCTGAACGA CCTGGAGCGT ATTGCACAGA GTGACTACAT CCCCACACAG
CAAGATGTGC TACGGACCCG CGTAAAGACC ACGGGGATCG TGGAGACACA CTTCACCTTC
AAGGACCTAC ACTTCAAGAT GTTTGATGTG GGTGGTCAGC GGTCTGAGCG GAAGAAGTGG
ATCCACTGCT TTGAGGGCGT CACAGCCATC ATCTTCTGCG TAGCCTTGAG CGCCTATGAC
TTGGTGCTAG CTGAGGACGA GGAGATGAAC CGCATGCATG AGAGCATGAA GCTATTCGAT
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG
GACCTGTTTG AGGAGAAGAT CACACACAGT CCCCTGACCA TCTGCTTCCC TGAGTACACA
GGGGCCAACA AATATGATGA GGCAGCCAGC TACATCCAGA GTAAGTTTGA GGACCTGAAT
AAGCGCAAAG ACACCAAGGA GATCTACACG CACTTCACGT GCGCCACCGA CACCAAGAAC
GTGCAGTTCG TGTTTGACGC CGTCACCGAT GTCATCATCA AGAACAACCT GAAGGACTGC
GGCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001269546.1
CDS262..1173
Misc Feature(1)202..204(+)
Misc Feature(2)262..1152(+)
Misc Feature(3)262..1137(+)
Misc Feature(4)316..753(+)
Misc Feature(5)634..660(+)
Misc Feature(6)649..1086(+)
Misc Feature(7)649..651(+)
Misc Feature(8)652..756(+)
Misc Feature(9)658..756(+)
Misc Feature(10)706..717(+)
Misc Feature(11)709..759(+)
Misc Feature(12)913..924(+)
Misc Feature(13)1057..1107(+)
Misc Feature(14)1081..1089(+)
Exon (1)1..223
Gene:GNAI2
Gene Synonym:
Exon (2)224..266
Gene:GNAI2
Gene Synonym:
Exon (3)267..408
Gene:GNAI2
Gene Synonym:
Exon (4)409..569
Gene:GNAI2
Gene Synonym:
Exon (5)570..698
Gene:GNAI2
Gene Synonym:
Exon (6)699..828
Gene:GNAI2
Gene Synonym:
Exon (7)829..982
Gene:GNAI2
Gene Synonym:
Exon (8)983..1197
Gene:GNAI2
Gene Synonym:
Exon (9)1198..2184
Gene:GNAI2
Gene Synonym:
Translation
Position Chain Variation Link
509 509 a, g dbSNP:770199226
579 579 c, t dbSNP:199645073
640 640 c, g, t dbSNP:137853226
641 641 a, g dbSNP:137853227
705 705 a, t dbSNP:137853228
820 820 a, g dbSNP:760792214
831 831 c, t dbSNP:267599879
955 955 c, t dbSNP:142945610
1368 1368 g, t dbSNP:781177917
1728 1728 a, g dbSNP:76340684
1866 1866 a, g dbSNP:745422685
2175 2175 c, g dbSNP:755622250

Target ORF information:

RefSeq Version NM_001282617
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282617

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGATCA TCCACGAGGA TGGCTACTCC GAGGAGGAAT GCCGGCAGTA CCGGGCGGTT 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATTGTCA AAGCCATGGG CAACCTGCAG
ATCGACTTTG CCGACCCCTC CAGAGCGGAC GACGCCAGGC AGCTATTTGC ACTGTCCTGC
ACCGCCGAGG AGCAAGGCGT GCTCCCTGAT GACCTGTCCG GCGTCATCCG GAGGCTCTGG
GCTGACCATG GTGTGCAGGC CTGCTTTGGC CGCTCAAGGG AATACCAGCT CAACGACTCA
GCTGCCTACT ACCTGAACGA CCTGGAGCGT ATTGCACAGA GTGACTACAT CCCCACACAG
CAAGATGTGC TACGGACCCG CGTAAAGACC ACGGGGATCG TGGAGACACA CTTCACCTTC
AAGGACCTAC ACTTCAAGAT GTTTGATGTG GGTGGTCAGC GGTCTGAGCG GAAGAAGTGG
ATCCACTGCT TTGAGGGCGT CACAGCCATC ATCTTCTGCG TAGCCTTGAG CGCCTATGAC
TTGGTGCTAG CTGAGGACGA GGAGATGAAC CGCATGCATG AGAGCATGAA GCTATTCGAT
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG
GACCTGTTTG AGGAGAAGAT CACACACAGT CCCCTGACCA TCTGCTTCCC TGAGTACACA
GGGGCCAACA AATATGATGA GGCAGCCAGC TACATCCAGA GTAAGTTTGA GGACCTGAAT
AAGCGCAAAG ACACCAAGGA GATCTACACG CACTTCACGT GCGCCACCGA CACCAAGAAC
GTGCAGTTCG TGTTTGACGC CGTCACCGAT GTCATCATCA AGAACAACCT GAAGGACTGC
GGCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14271
Accession Version NM_001282618.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037331.1, BC012138.1, AK055574.1 and AK096595.1. Summary: The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCATTG TCAAAGCCAT GGGCAACCTG CAGATCGACT TTGCCGACCC CTCCAGAGCG 
GACGACGCCA GGCAGCTATT TGCACTGTCC TGCACCGCCG AGGAGCAAGG CGTGCTCCCT
GATGACCTGT CCGGCGTCAT CCGGAGGCTC TGGGCTGACC ATGGTGTGCA GGCCTGCTTT
GGCCGCTCAA GGGAATACCA GCTCAACGAC TCAGCTGCCT ACTACCTGAA CGACCTGGAG
CGTATTGCAC AGAGTGACTA CATCCCCACA CAGCAAGATG TGCTACGGAC CCGCGTAAAG
ACCACGGGGA TCGTGGAGAC ACACTTCACC TTCAAGGACC TACACTTCAA GATGTTTGAT
GTGGGTGGTC AGCGGTCTGA GCGGAAGAAG TGGATCCACT GCTTTGAGGG CGTCACAGCC
ATCATCTTCT GCGTAGCCTT GAGCGCCTAT GACTTGGTGC TAGCTGAGGA CGAGGAGATG
AACCGCATGC ATGAGAGCAT GAAGCTATTC GATAGCATCT GCAACAACAA GTGGTTCACA
GACACGTCCA TCATCCTCTT CCTCAACAAG AAGGACCTGT TTGAGGAGAA GATCACACAC
AGTCCCCTGA CCATCTGCTT CCCTGAGTAC ACAGGGGCCA ACAAATATGA TGAGGCAGCC
AGCTACATCC AGAGTAAGTT TGAGGACCTG AATAAGCGCA AAGACACCAA GGAGATCTAC
ACGCACTTCA CGTGCGCCAC CGACACCAAG AACGTGCAGT TCGTGTTTGA CGCCGTCACC
GATGTCATCA TCAAGAACAA CCTGAAGGAC TGCGGCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001269547.1
CDS380..1204
Misc Feature(1)227..229(+)
Misc Feature(2)383..1195(+)
Misc Feature(3)383..1183(+)
Misc Feature(4)389..784(+)
Misc Feature(5)665..691(+)
Misc Feature(6)680..1117(+)
Misc Feature(7)680..682(+)
Misc Feature(8)683..787(+)
Misc Feature(9)689..787(+)
Misc Feature(10)737..748(+)
Misc Feature(11)740..790(+)
Misc Feature(12)944..955(+)
Misc Feature(13)1088..1138(+)
Misc Feature(14)1112..1120(+)
Exon (1)1..297
Gene:GNAI2
Gene Synonym:
Exon (2)298..439
Gene:GNAI2
Gene Synonym:
Exon (3)440..600
Gene:GNAI2
Gene Synonym:
Exon (4)601..729
Gene:GNAI2
Gene Synonym:
Exon (5)730..859
Gene:GNAI2
Gene Synonym:
Exon (6)860..1013
Gene:GNAI2
Gene Synonym:
Exon (7)1014..1228
Gene:GNAI2
Gene Synonym:
Exon (8)1229..2215
Gene:GNAI2
Gene Synonym:
Translation
Position Chain Variation Link
540 540 a, g dbSNP:770199226
610 610 c, t dbSNP:199645073
671 671 c, g, t dbSNP:137853226
672 672 a, g dbSNP:137853227
736 736 a, t dbSNP:137853228
851 851 a, g dbSNP:760792214
862 862 c, t dbSNP:267599879
986 986 c, t dbSNP:142945610
1399 1399 g, t dbSNP:781177917
1759 1759 a, g dbSNP:76340684
1897 1897 a, g dbSNP:745422685
2206 2206 c, g dbSNP:755622250

Target ORF information:

RefSeq Version NM_001282618
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282618

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCATTG TCAAAGCCAT GGGCAACCTG CAGATCGACT TTGCCGACCC CTCCAGAGCG 
GACGACGCCA GGCAGCTATT TGCACTGTCC TGCACCGCCG AGGAGCAAGG CGTGCTCCCT
GATGACCTGT CCGGCGTCAT CCGGAGGCTC TGGGCTGACC ATGGTGTGCA GGCCTGCTTT
GGCCGCTCAA GGGAATACCA GCTCAACGAC TCAGCTGCCT ACTACCTGAA CGACCTGGAG
CGTATTGCAC AGAGTGACTA CATCCCCACA CAGCAAGATG TGCTACGGAC CCGCGTAAAG
ACCACGGGGA TCGTGGAGAC ACACTTCACC TTCAAGGACC TACACTTCAA GATGTTTGAT
GTGGGTGGTC AGCGGTCTGA GCGGAAGAAG TGGATCCACT GCTTTGAGGG CGTCACAGCC
ATCATCTTCT GCGTAGCCTT GAGCGCCTAT GACTTGGTGC TAGCTGAGGA CGAGGAGATG
AACCGCATGC ATGAGAGCAT GAAGCTATTC GATAGCATCT GCAACAACAA GTGGTTCACA
GACACGTCCA TCATCCTCTT CCTCAACAAG AAGGACCTGT TTGAGGAGAA GATCACACAC
AGTCCCCTGA CCATCTGCTT CCCTGAGTAC ACAGGGGCCA ACAAATATGA TGAGGCAGCC
AGCTACATCC AGAGTAAGTT TGAGGACCTG AATAAGCGCA AAGACACCAA GGAGATCTAC
ACGCACTTCA CGTGCGCCAC CGACACCAAG AACGTGCAGT TCGTGTTTGA CGCCGTCACC
GATGTCATCA TCAAGAACAA CCTGAAGGAC TGCGGCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14225
Accession Version NM_001282619.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA528337.1, BC016995.1 and AK055574.1. Summary: The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016995.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGTATG CAGGGCATCT TCCTGCCAGC TCTGCCCAGG GCACCATATT GGCATGCACC 
AGCTGCACAG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001269548.1
CDS561..1580
Misc Feature(1)519..521(+)
Misc Feature(2)630..1571(+)
Misc Feature(3)630..1559(+)
Misc Feature(4)630..1493(+)
Misc Feature(5)630..653(+)
Misc Feature(6)633..1160(+)
Misc Feature(7)1041..1067(+)
Misc Feature(8)1056..1058(+)
Misc Feature(9)1059..1163(+)
Misc Feature(10)1065..1163(+)
Misc Feature(11)1113..1124(+)
Misc Feature(12)1116..1166(+)
Misc Feature(13)1320..1331(+)
Misc Feature(14)1464..1514(+)
Misc Feature(15)1488..1496(+)
Exon (1)1..506
Gene:GNAI2
Gene Synonym:
Exon (2)507..630
Gene:GNAI2
Gene Synonym:
Exon (3)631..673
Gene:GNAI2
Gene Synonym:
Exon (4)674..815
Gene:GNAI2
Gene Synonym:
Exon (5)816..976
Gene:GNAI2
Gene Synonym:
Exon (6)977..1105
Gene:GNAI2
Gene Synonym:
Exon (7)1106..1235
Gene:GNAI2
Gene Synonym:
Exon (8)1236..1389
Gene:GNAI2
Gene Synonym:
Exon (9)1390..1604
Gene:GNAI2
Gene Synonym:
Exon (10)1605..2591
Gene:GNAI2
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, c dbSNP:759575969
40 40 a, c dbSNP:587711157
69 69 a, g dbSNP:587771159
73 73 c, t dbSNP:765523000
82 82 a, g dbSNP:587646002
109 109 a, g dbSNP:757413671
118 118 c, g dbSNP:587703054
199 199 g, t dbSNP:150038491
211 211 c, g dbSNP:587756537
211 211 c, g dbSNP:760953439
291 291 a, c dbSNP:587640496
322 322 c, t dbSNP:587714005
330 330 a, g dbSNP:587752982
331 331 a, g dbSNP:587650952
389 389 a, g dbSNP:147710722
419 419 -, aga dbSNP:587725421
419 419 -, aga dbSNP:766280212
422 422 -, g dbSNP:748371795
442 442 a, g dbSNP:758617290
455 455 a, g dbSNP:769726896
458 458 c, t dbSNP:765262171
489 489 a, c dbSNP:191549028
916 916 a, g dbSNP:770199226
986 986 c, t dbSNP:199645073
1047 1047 c, g, t dbSNP:137853226
1048 1048 a, g dbSNP:137853227
1112 1112 a, t dbSNP:137853228
1227 1227 a, g dbSNP:760792214
1238 1238 c, t dbSNP:267599879
1362 1362 c, t dbSNP:142945610
1775 1775 g, t dbSNP:781177917
2135 2135 a, g dbSNP:76340684
2273 2273 a, g dbSNP:745422685
2582 2582 c, g dbSNP:755622250

Target ORF information:

RefSeq Version NM_001282619
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGTATG CAGGGCATCT TCCTGCCAGC TCTGCCCAGG GCACCATATT GGCATGCACC 
AGCTGCACAG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14309
Accession Version NM_001282620.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA528337.1, AK096595.1 and AK055574.1. On Sep 14, 2013 this sequence version replaced gi:530372311. Summary: The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (6) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096595.1, AK096299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACGGAGG GTGTGAAGAC GCTAGGCTGG ACGAAGCAGA AAGGCGGGTG TCACTGGGGA 
CGTTCTGAGG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001269549.1
CDS437..1456
Misc Feature(1)404..406(+)
Misc Feature(2)506..1447(+)
Misc Feature(3)506..1435(+)
Misc Feature(4)506..1369(+)
Misc Feature(5)506..529(+)
Misc Feature(6)509..1036(+)
Misc Feature(7)917..943(+)
Misc Feature(8)932..934(+)
Misc Feature(9)935..1039(+)
Misc Feature(10)941..1039(+)
Misc Feature(11)989..1000(+)
Misc Feature(12)992..1042(+)
Misc Feature(13)1196..1207(+)
Misc Feature(14)1340..1390(+)
Misc Feature(15)1364..1372(+)
Exon (1)1..506
Gene:GNAI2
Gene Synonym:
Exon (2)507..549
Gene:GNAI2
Gene Synonym:
Exon (3)550..691
Gene:GNAI2
Gene Synonym:
Exon (4)692..852
Gene:GNAI2
Gene Synonym:
Exon (5)853..981
Gene:GNAI2
Gene Synonym:
Exon (6)982..1111
Gene:GNAI2
Gene Synonym:
Exon (7)1112..1265
Gene:GNAI2
Gene Synonym:
Exon (8)1266..1480
Gene:GNAI2
Gene Synonym:
Exon (9)1481..2467
Gene:GNAI2
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, c dbSNP:759575969
40 40 a, c dbSNP:587711157
69 69 a, g dbSNP:587771159
73 73 c, t dbSNP:765523000
82 82 a, g dbSNP:587646002
109 109 a, g dbSNP:757413671
118 118 c, g dbSNP:587703054
199 199 g, t dbSNP:150038491
211 211 c, g dbSNP:587756537
211 211 c, g dbSNP:760953439
291 291 a, c dbSNP:587640496
322 322 c, t dbSNP:587714005
330 330 a, g dbSNP:587752982
331 331 a, g dbSNP:587650952
389 389 a, g dbSNP:147710722
419 419 -, aga dbSNP:587725421
419 419 -, aga dbSNP:766280212
422 422 -, g dbSNP:748371795
442 442 a, g dbSNP:758617290
455 455 a, g dbSNP:769726896
458 458 c, t dbSNP:765262171
489 489 a, c dbSNP:191549028
792 792 a, g dbSNP:770199226
862 862 c, t dbSNP:199645073
923 923 c, g, t dbSNP:137853226
924 924 a, g dbSNP:137853227
988 988 a, t dbSNP:137853228
1103 1103 a, g dbSNP:760792214
1114 1114 c, t dbSNP:267599879
1238 1238 c, t dbSNP:142945610
1651 1651 g, t dbSNP:781177917
2011 2011 a, g dbSNP:76340684
2149 2149 a, g dbSNP:745422685
2458 2458 c, g dbSNP:755622250

Target ORF information:

RefSeq Version NM_001282620
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282620

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACGGAGG GTGTGAAGAC GCTAGGCTGG ACGAAGCAGA AAGGCGGGTG TCACTGGGGA 
CGTTCTGAGG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13581
Accession Version NM_001166425.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB044421.1, AK302330.1, AK126708.1 and AK055574.1. Summary: The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGAGGTG CTGGGGAGTC AGGGAAGAGC ACCATCGTCA AGCAGATGAA GATCATCCAC 
GAGGATGGCT ACTCCGAGGA GGAATGCCGG CAGTACCGGG CGGTTGTCTA CAGCAACACC
ATCCAGTCCA TCATGGCCAT TGTCAAAGCC ATGGGCAACC TGCAGATCGA CTTTGCCGAC
CCCTCCAGAG CGGACGACGC CAGGCAGCTA TTTGCACTGT CCTGCACCGC CGAGGAGCAA
GGCGTGCTCC CTGATGACCT GTCCGGCGTC ATCCGGAGGC TCTGGGCTGA CCATGGTGTG
CAGGCCTGCT TTGGCCGCTC AAGGGAATAC CAGCTCAACG ACTCAGCTGC CTACTACCTG
AACGACCTGG AGCGTATTGC ACAGAGTGAC TACATCCCCA CACAGCAAGA TGTGCTACGG
ACCCGCGTAA AGACCACGGG GATCGTGGAG ACACACTTCA CCTTCAAGGA CCTACACTTC
AAGATGTTTG ATGTGGGTGG TCAGCGGTCT GAGCGGAAGA AGTGGATCCA CTGCTTTGAG
GGCGTCACAG CCATCATCTT CTGCGTAGCC TTGAGCGCCT ATGACTTGGT GCTAGCTGAG
GACGAGGAGA TGAACCGCAT GCATGAGAGC ATGAAGCTAT TCGATAGCAT CTGCAACAAC
AAGTGGTTCA CAGACACGTC CATCATCCTC TTCCTCAACA AGAAGGACCT GTTTGAGGAG
AAGATCACAC ACAGTCCCCT GACCATCTGC TTCCCTGAGT ACACAGGGGC CAACAAATAT
GATGAGGCAG CCAGCTACAT CCAGAGTAAG TTTGAGGACC TGAATAAGCG CAAAGACACC
AAGGAGATCT ACACGCACTT CACGTGCGCC ACCGACACCA AGAACGTGCA GTTCGTGTTT
GACGCCGTCA CCGATGTCAT CATCAAGAAC AACCTGAAGG ACTGCGGCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001159897.1
CDS176..1132
Misc Feature(1)137..139(+)
Misc Feature(2)182..1123(+)
Misc Feature(3)182..1111(+)
Misc Feature(4)182..1045(+)
Misc Feature(5)182..205(+)
Misc Feature(6)185..712(+)
Misc Feature(7)593..619(+)
Misc Feature(8)608..610(+)
Misc Feature(9)611..715(+)
Misc Feature(10)617..715(+)
Misc Feature(11)665..676(+)
Misc Feature(12)668..718(+)
Misc Feature(13)872..883(+)
Misc Feature(14)1016..1066(+)
Misc Feature(15)1040..1048(+)
Exon (1)1..182
Gene:GNAI2
Gene Synonym:
Exon (2)183..225
Gene:GNAI2
Gene Synonym:
Exon (3)226..367
Gene:GNAI2
Gene Synonym:
Exon (4)368..528
Gene:GNAI2
Gene Synonym:
Exon (5)529..657
Gene:GNAI2
Gene Synonym:
Exon (6)658..787
Gene:GNAI2
Gene Synonym:
Exon (7)788..941
Gene:GNAI2
Gene Synonym:
Exon (8)942..1156
Gene:GNAI2
Gene Synonym:
Exon (9)1157..2143
Gene:GNAI2
Gene Synonym:
Translation
Position Chain Variation Link
138 138 a, g dbSNP:200422517
468 468 a, g dbSNP:770199226
538 538 c, t dbSNP:199645073
599 599 c, g, t dbSNP:137853226
600 600 a, g dbSNP:137853227
664 664 a, t dbSNP:137853228
779 779 a, g dbSNP:760792214
790 790 c, t dbSNP:267599879
914 914 c, t dbSNP:142945610
1327 1327 g, t dbSNP:781177917
1687 1687 a, g dbSNP:76340684
1825 1825 a, g dbSNP:745422685
2134 2134 c, g dbSNP:755622250

Target ORF information:

RefSeq Version NM_001166425
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166425

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGAGGTG CTGGGGAGTC AGGGAAGAGC ACCATCGTCA AGCAGATGAA GATCATCCAC 
GAGGATGGCT ACTCCGAGGA GGAATGCCGG CAGTACCGGG CGGTTGTCTA CAGCAACACC
ATCCAGTCCA TCATGGCCAT TGTCAAAGCC ATGGGCAACC TGCAGATCGA CTTTGCCGAC
CCCTCCAGAG CGGACGACGC CAGGCAGCTA TTTGCACTGT CCTGCACCGC CGAGGAGCAA
GGCGTGCTCC CTGATGACCT GTCCGGCGTC ATCCGGAGGC TCTGGGCTGA CCATGGTGTG
CAGGCCTGCT TTGGCCGCTC AAGGGAATAC CAGCTCAACG ACTCAGCTGC CTACTACCTG
AACGACCTGG AGCGTATTGC ACAGAGTGAC TACATCCCCA CACAGCAAGA TGTGCTACGG
ACCCGCGTAA AGACCACGGG GATCGTGGAG ACACACTTCA CCTTCAAGGA CCTACACTTC
AAGATGTTTG ATGTGGGTGG TCAGCGGTCT GAGCGGAAGA AGTGGATCCA CTGCTTTGAG
GGCGTCACAG CCATCATCTT CTGCGTAGCC TTGAGCGCCT ATGACTTGGT GCTAGCTGAG
GACGAGGAGA TGAACCGCAT GCATGAGAGC ATGAAGCTAT TCGATAGCAT CTGCAACAAC
AAGTGGTTCA CAGACACGTC CATCATCCTC TTCCTCAACA AGAAGGACCT GTTTGAGGAG
AAGATCACAC ACAGTCCCCT GACCATCTGC TTCCCTGAGT ACACAGGGGC CAACAAATAT
GATGAGGCAG CCAGCTACAT CCAGAGTAAG TTTGAGGACC TGAATAAGCG CAAAGACACC
AAGGAGATCT ACACGCACTT CACGTGCGCC ACCGACACCA AGAACGTGCA GTTCGTGTTT
GACGCCGTCA CCGATGTCAT CATCAAGAAC AACCTGAAGG ACTGCGGCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Galphai2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation
Mol. Cell. Biol. 34 (4), 619-630 (2014)
Minetti GC, Feige JN, Bombard F, Heier A, Morvan F, Nurnberg B, Leiss V, Birnbaumer L, Glass DJ and Fornaro M.


book

Ubiquitination of the heterotrimeric G protein alpha subunits Galphai2 and Galphaq is prevented by the guanine nucleotide exchange factor Ric-8A
Biochem. Biophys. Res. Commun. 435 (3), 414-419 (2013)
Chishiki K, Kamakura S, Yuzawa S, Hayase J and Sumimoto H.


book

Increased Gsalpha within blood cell membrane lipid microdomains in some depressive disorders: an exploratory study
J Psychiatr Res 47 (6), 706-711 (2013)
Mooney JJ, Samson JA, McHale NL, Pappalarado KM, Alpert JE and Schildkraut JJ.


book

The familial hemiplegic migraine type 1 mutation K1336E affects direct G protein-mediated regulation of neuronal P/Q-type Ca2+ channels
Cephalalgia 33 (6), 398-407 (2013)
Garza-Lopez E, Gonzalez-Ramirez R, Gandini MA, Sandoval A and Felix R.


book

HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Galpha(i2) for degradation through ubiquitination
J. Biol. Chem. 287 (49), 41481-41498 (2012)
Chandrasekaran P, Buckley M, Moore V, Wang LQ, Kehrl JH and Venkatesan S.


book

Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12p12-p13
Genomics 12 (1), 125-129 (1992)
Magovcevic I, Ang SL, Seidman JG, Tolman CJ, Neer EJ and Morton CC.


book

Identification of G protein alpha-subunits in RINm5F cells and their selective interaction with galanin receptor
Diabetes 40 (9), 1170-1176 (1991)
Cormont M, Le Marchand-Brustel Y, Van Obberghen E, Spiegel AM and Sharp GW.


book

Evidence for opioid receptor-mediated activation of the G-proteins, Go and Gi2, in membranes of neuroblastoma x glioma (NG108-15) hybrid cells
J. Biol. Chem. 266 (6), 3365-3368 (1991)
Offermanns S, Schultz G and Rosenthal W.


book

Two G protein oncogenes in human endocrine tumors
Science 249 (4969), 655-659 (1990)
Lyons J, Landis CA, Harsh G, Vallar L, Grunewald K, Feichtinger H, Duh QY, Clark OH, Kawasaki E and Bourne HR.


book

Hormonal regulation of Gi2 alpha-subunit phosphorylation in intact hepatocytes
Biochem. J. 268 (2), 449-457 (1990)
Bushfield M, Murphy GJ, Lavan BE, Parker PJ, Hruby VJ, Milligan G and Houslay MD.


 
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